恒磁场对SD大鼠深创面愈合的影响
摘要
目的
探讨恒磁场对SD大鼠深创面愈合的影响;探讨恒磁场对创面愈合过程中VEGF表达的影响;探讨不同强度恒磁场治疗SD大鼠深创面的区别。
方法
将48只清洁级SD大鼠随机分成0.16T磁疗组、0.32T磁疗组和对照组。分别在术后第3、6、9、12天每组处死4只大鼠,大体观察创面情况;测定创面愈合指数;对肉芽组织进行病理组织学观察;免疫组化检测肉芽组织VEGF表达。使用统计软件SPSS 12.0作统计学处理,计量数据处理以X±s表示,样本均数之间的比较用t检验。等级资料用数值变量(N)表示,两个样本之间的比较采用Wilcoxon秩和检验。
结果
大体观察:在整个愈合过程中,0.16T磁疗组、0.32T磁疗组创面渗出多,肉芽生长快。上皮出现早,愈合时间短。0.32T磁疗组与0.16T磁疗组相比,创面渗出物量更多,肉芽生长更加旺盛,上皮出现及愈合时间也略早些。对照组创面渗出少,肉芽生长量少且缓慢,上皮出现较迟,创面愈合明显比磁疗组慢。愈合指数:磁疗组在第3、6、9、12天时的愈合指数明显高于对照组,差异有统计学意义。0.32T磁疗组高于0.16T磁疗组,差异有统计学意义。组织学观察早期磁疗组可见毛细血管数量较多,管腔较粗,成纤维细胞数量多,胞体大,肉芽组织层较厚,以0.32T磁疗组最明显。对照组毛细血管较少,且管腔狭窄,成纤维细胞数量也较少。中期磁疗组创面肉芽组织毛细血管数量更多,管腔扩大,毛细血管内皮细胞体积较大,有较多成纤维细胞变为纤维细胞,创缘上皮组织明显增生。对照组毛细血管、成纤维细胞及胶原纤维量少,毛细血管管腔较狭窄,且胶原纤维粗细不均、排列紊乱,创缘上皮组织细胞少。晚期磁疗组毛细血管较9天时明显减少,成纤维细胞也减少,胶原纤维进一步增多。上皮爬行明显,接近闭合。对照组毛细血管也有所减少,成纤维细胞较多。胶原纤维也进一步增多,但仍少于磁疗组,上皮爬行及分化也弱于磁疗组。在创面愈合过程中,毛细血管数及成纤维细胞数磁疗组与对照组相比差异有统计学意义,0.32T磁疗组与0.16T磁疗组相比差异有统计学意义。
创面修复不同时期VEGF表达观察结果:在术后第3、6天磁疗组VEGF表达强于对照组,差异有统计学意义。第9、12天磁疗组与对照组相比,差异无统计学意义(P＞0.05)。0.32T磁疗组VEGF表达阳性率在第3、6天明显高于0.16T磁疗组,差异有统计学意义。两个磁疗组在第6天时VEGF阳性率达到整个愈合过程中的峰值,而对照组在第9天时才达到高峰,且VEGF表达强度低于两个磁疗组。
结论
1、0.16T恒磁片及0.32T恒磁片均能促进SD大鼠深创面的愈合;
2、恒磁场促进深创面愈合的机制可能和在创面愈合早、中期增强VEGF的表达有关;
3、应用0.32T恒磁片能使SD大鼠深创面在愈合过程早、中期VEGF表达高于应用0.16T恒磁片的创面,可能和磁场强度越高,促进成纤维细胞和血管内皮细胞产生定向移动的速度越快有关。
Objective:To investigate the effect of static magnetic field on deep wound healing of SD rats;to investigate the effect of static magnetic field on VEGF during the course of wound healing;to investigate the effect of different strength static magnetic field on deep wound healing of SD rats.
Methods:Dividing forty-eight SD rats into three groups:group with 0.16T magnetic disk treated(0.16Tgroup);group with 0.32T magnetic disk treated(0.32Tgroup);control group.General wounds healing situation was observated on the third sixth ninth twelfth day ;The erea of every wound was calculated;Every granulation tissue sample was observated by tissue-pathology.The tissue of granulation was dyeing by immune tissue chemical decoration method,in which VEGF protein content with its range in tissue was measured.
Results:General observation showed:There were more oozed thing and granulation in the wounds of 0.16T group and 0.32T group than in that of control group during the course of wound healing.Epithelium cell emerged more early in magnetic group than in control group. The healing time of magnetic group was shorter than of control group.Contrast 0.32T group to 0.16T group,there were more oozed thing and granulation in the wounds.The healing index of magnetic group wounds were larger than that of control group on 3rd,6th,9th and 12th day. The healing index of 0.32T group wounds were larger than that of 0.16T group wounds.Observation of histology indicated:There were more micrangium and fibroblast in magnetic group wounds than that in control group wounds during early time.In the 0.32T group were there the most micrangium and fibroblast.It was more obviously during the middle time.
There were lesser micrangium and fibroblast in magnetic group on 12th day than that on 9th day.And more collagen fibers emerged. Epithelium tissue crawled obviously.It was almost closed.The amount of micrangium and fibroblast were more in magnetic group wounds than that in control group wounds.The difference has statistical significance.It was also between 0.32T group and 0.16T group.Observation of VEGF at the course of wound healing:The expressing of VEGF in magnetic group wounds on 3rd and 6th was stronger than in control group wounds.The difference between them has statistical significance.While there were no obvious difference between them on 9th and 12th day(P>0.05).The expressing of VEGF in 0.32T group on 3rd and 6th day was stronger than in 0.16T group. The difference between them has statistical significance.The expressing strength of VEGF in magnetic group reached the peak amplitude on the 6th day.That in control group reached peak amplitude on 9th day.And the peak amplitude of magnetic group was stronger than that of control group.
Conclusion:
1、Static magnetic disc of 0.16T and 0.32T can promote deep woundof SD rats heal.
2、The mechanism of static magnetic field promoting wound heal maybe relative to the expressing highly of VEGF during early and middle time.
3、The expressing of VEGF in 0.32T group wounds during early andmiddle time was stronger than that in 0.16T group wounds.lt may be because that the strong magnetic field promote directional migration of fibroblast and vascular endothelial cell quickly.
探讨恒磁场对SD大鼠深创面愈合的影响;探讨恒磁场对创面愈合过程中VEGF表达的影响;探讨不同强度恒磁场治疗SD大鼠深创面的区别。
方法
将48只清洁级SD大鼠随机分成0.16T磁疗组、0.32T磁疗组和对照组。分别在术后第3、6、9、12天每组处死4只大鼠,大体观察创面情况;测定创面愈合指数;对肉芽组织进行病理组织学观察;免疫组化检测肉芽组织VEGF表达。使用统计软件SPSS 12.0作统计学处理,计量数据处理以X±s表示,样本均数之间的比较用t检验。等级资料用数值变量(N)表示,两个样本之间的比较采用Wilcoxon秩和检验。
结果
大体观察:在整个愈合过程中,0.16T磁疗组、0.32T磁疗组创面渗出多,肉芽生长快。上皮出现早,愈合时间短。0.32T磁疗组与0.16T磁疗组相比,创面渗出物量更多,肉芽生长更加旺盛,上皮出现及愈合时间也略早些。对照组创面渗出少,肉芽生长量少且缓慢,上皮出现较迟,创面愈合明显比磁疗组慢。愈合指数:磁疗组在第3、6、9、12天时的愈合指数明显高于对照组,差异有统计学意义。0.32T磁疗组高于0.16T磁疗组,差异有统计学意义。组织学观察早期磁疗组可见毛细血管数量较多,管腔较粗,成纤维细胞数量多,胞体大,肉芽组织层较厚,以0.32T磁疗组最明显。对照组毛细血管较少,且管腔狭窄,成纤维细胞数量也较少。中期磁疗组创面肉芽组织毛细血管数量更多,管腔扩大,毛细血管内皮细胞体积较大,有较多成纤维细胞变为纤维细胞,创缘上皮组织明显增生。对照组毛细血管、成纤维细胞及胶原纤维量少,毛细血管管腔较狭窄,且胶原纤维粗细不均、排列紊乱,创缘上皮组织细胞少。晚期磁疗组毛细血管较9天时明显减少,成纤维细胞也减少,胶原纤维进一步增多。上皮爬行明显,接近闭合。对照组毛细血管也有所减少,成纤维细胞较多。胶原纤维也进一步增多,但仍少于磁疗组,上皮爬行及分化也弱于磁疗组。在创面愈合过程中,毛细血管数及成纤维细胞数磁疗组与对照组相比差异有统计学意义,0.32T磁疗组与0.16T磁疗组相比差异有统计学意义。
创面修复不同时期VEGF表达观察结果:在术后第3、6天磁疗组VEGF表达强于对照组,差异有统计学意义。第9、12天磁疗组与对照组相比,差异无统计学意义(P＞0.05)。0.32T磁疗组VEGF表达阳性率在第3、6天明显高于0.16T磁疗组,差异有统计学意义。两个磁疗组在第6天时VEGF阳性率达到整个愈合过程中的峰值,而对照组在第9天时才达到高峰,且VEGF表达强度低于两个磁疗组。
结论
1、0.16T恒磁片及0.32T恒磁片均能促进SD大鼠深创面的愈合;
2、恒磁场促进深创面愈合的机制可能和在创面愈合早、中期增强VEGF的表达有关;
3、应用0.32T恒磁片能使SD大鼠深创面在愈合过程早、中期VEGF表达高于应用0.16T恒磁片的创面,可能和磁场强度越高,促进成纤维细胞和血管内皮细胞产生定向移动的速度越快有关。
Objective:To investigate the effect of static magnetic field on deep wound healing of SD rats;to investigate the effect of static magnetic field on VEGF during the course of wound healing;to investigate the effect of different strength static magnetic field on deep wound healing of SD rats.
Methods:Dividing forty-eight SD rats into three groups:group with 0.16T magnetic disk treated(0.16Tgroup);group with 0.32T magnetic disk treated(0.32Tgroup);control group.General wounds healing situation was observated on the third sixth ninth twelfth day ;The erea of every wound was calculated;Every granulation tissue sample was observated by tissue-pathology.The tissue of granulation was dyeing by immune tissue chemical decoration method,in which VEGF protein content with its range in tissue was measured.
Results:General observation showed:There were more oozed thing and granulation in the wounds of 0.16T group and 0.32T group than in that of control group during the course of wound healing.Epithelium cell emerged more early in magnetic group than in control group. The healing time of magnetic group was shorter than of control group.Contrast 0.32T group to 0.16T group,there were more oozed thing and granulation in the wounds.The healing index of magnetic group wounds were larger than that of control group on 3rd,6th,9th and 12th day. The healing index of 0.32T group wounds were larger than that of 0.16T group wounds.Observation of histology indicated:There were more micrangium and fibroblast in magnetic group wounds than that in control group wounds during early time.In the 0.32T group were there the most micrangium and fibroblast.It was more obviously during the middle time.
There were lesser micrangium and fibroblast in magnetic group on 12th day than that on 9th day.And more collagen fibers emerged. Epithelium tissue crawled obviously.It was almost closed.The amount of micrangium and fibroblast were more in magnetic group wounds than that in control group wounds.The difference has statistical significance.It was also between 0.32T group and 0.16T group.Observation of VEGF at the course of wound healing:The expressing of VEGF in magnetic group wounds on 3rd and 6th was stronger than in control group wounds.The difference between them has statistical significance.While there were no obvious difference between them on 9th and 12th day(P>0.05).The expressing of VEGF in 0.32T group on 3rd and 6th day was stronger than in 0.16T group. The difference between them has statistical significance.The expressing strength of VEGF in magnetic group reached the peak amplitude on the 6th day.That in control group reached peak amplitude on 9th day.And the peak amplitude of magnetic group was stronger than that of control group.
Conclusion:
1、Static magnetic disc of 0.16T and 0.32T can promote deep woundof SD rats heal.
2、The mechanism of static magnetic field promoting wound heal maybe relative to the expressing highly of VEGF during early and middle time.
3、The expressing of VEGF in 0.32T group wounds during early andmiddle time was stronger than that in 0.16T group wounds.lt may be because that the strong magnetic field promote directional migration of fibroblast and vascular endothelial cell quickly.
引文
[1]熊伟,肖萍.稀土钴永磁贴敷对伤口愈合的实验报告[J].中华生物磁学.1989,3(1-2):104.
[2]罗二平,申广浩,周龙甫,等.稳恒0.2T磁场对兔创伤修复的影响[J].第四军医大学学报.2004,25(13):1191-1193.
[3]王军学,罗二平,申广浩,等.恒磁场对临床术后伤口愈合的影响[J].第四军医大学学报.2006,27(8):754-756.
[4]Cribbs RK,Luguetts MH,Besner GE.Acceleration of pardal thickness bum wound healing with topical application of heparin-binding EGF-like growth factor(HB-EGF).J Bwn Care Rehatic.1998;19:95.
[5]李爱冰,袁先厚,陈新军.VEGF bFGF在血管网织细胞瘤中的表达.中国临床神经外科杂志.2000,5(4):229-231.
[6]Leung DW,Cachines G,Kuang WJ,et al.Vascular endothelial growth factor issecreted angiogenic mitogen[J].Science,1989,246:1306.
[7]Terman BI,DougherV,Ermazen M,et al.Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor[J].Biochem Biophys Res Commun,1992,187:1579.
[8]Okano h,Onmori R,Tomita N,et al.Effects of a moderate- intensity static magnetic field on VEGF-A stimulsted endothelial capillary tuble formation in vitro.Bioelectromagnetics,2006 Dec;27(8):6284FI0.
[9]Dvorak HK.Angiogenesis:update 2005[J].J Thmmb Haemost,2005,3(8):1835-1842.
[10]彭湃,郭树忠,韩岩,等.VEGF对创伤组织中KDR,bFGF和PDGF mRNA 表达的影响.中国美容医学,2004,13(3):266-268.
[11]许伟榕,王莉,赵涵芳,等.人血管内皮生长因子基因工程生物膜愈合大鼠皮肤创伤的初步研究.外科理论与实践,2006,11(3):244-247.
[12]赵雷,王莉,姜叙诚,等.hVEGF基因工程生物膜对全层皮肤缺损创面愈合的影响上海交通大学学报(医学版),2006,26(9):1015-1018.
[13]彭瑞云,王德文,高亚兵,等.大鼠皮肤伤口内VEGF基因表达的定量病理研究.中国体视学与图像分析.2001,6(2):95-98.
[14]彭瑞云,高亚兵,熊程奇,等.正常和放射伤口血管再生中VEGF基因的表达及其意义.细胞与分子免疫学杂志.2001,17(5):410-412.
[15]Dvorak HF,Brown LF,Detmar M,et al.Vascular permeability factOr/vascular endothelial growth factor.Microvascular hyperpermeability,and angiogenesis.Am J Pathol.1995;146(5):1029.
[16]杨述华,周迎春,杨操,等.血管内皮生长因子基因转染促进伤口 愈合的实验研究.中华骨科杂志,2001,21(5):301-304.
[17]谢卫国,Werner Lindenmaier,Stefan Gryzybowsk,等.血管内皮生长因子基因修饰对裸鼠移植皮肤替代物的影响.中华烧伤杂志,2005,21(3):203-206.
[18]刘亮,刘旭胜.巨噬细胞在创伤愈合血管生成中作用的研究进展.中华烧伤杂志.2004,20(4):249-251.
[19]陈欣,副岛一孝,野崎斡弘,等.成纤维细胞移植促进人工真皮内血管新生的研究.中国修复重建外科杂志.2004,18(3):205-208.
[1]Bassett CA.Tibial fracture healing due to constant electrieal field.J.Bone Joint Sury,1977-53(A):1655.
[2]Dehass WG.The effects of low frequency magnetic fields on the healing of the rabbit.Clin Orthop.1979.145:245.
[3]Banal.The classic fudumenlal aspects of fracture.Clin Orthop.1977,124-125.
[4]刘海起,朱显,赵士林,等.磁与自然铜促进骨折愈合的初步实验研究.中华外科杂志,1983,21(1):1-4.
[5]May HU,Nippel FJ,Hansjurgens A,et al.Acceleration of ossification by means of interferential current.Prog Clin Biol Res.1985;187:469-478.
[6]Brighton CT,Hozack WJ,Brager MD,et al.Fracture healing in the rabbit fibula when subjected to various capacitively coupled electrical fields.J Orthop Res.1985;3(3):331-340.
[7]李仁杰,柳用墨,王崇武.旋磁场促进骨折愈合的初步实验研究[J].中华骨科杂志.1988,8(6):445.
[8]崔保义,钟锐球.磁场促进“骨不连”和“延迟愈合”性骨折愈合研究[J].中国磁学磁性材料杂志,1990,11(18):153.
[9]崔保义,钟锐球.磁场促进家兔骨折愈合部分病理学研究[J].中国磁性材料杂志,1993,12(27):747.
[10]续力民,康树林,李海亭,等.体内磁场促进骨折愈合的实验研究[J].北京军区医药,1994(5):329-331.
[11]Deibert MC,Mcleod BR,Smith SD,et al.Ion responance electromagnetic field stimulation of fracture healing in rabbits with a fibular ostecomy.J Orthop Res.1994Nov;12(6):878-885.
[12]Zhuang H,Wang W,Seldes RM,et al.Electrical stimulation induces the level of TGF-β1 mRNA in osteoblaetic cells by a mechanism involving calcium/calmodulin paathway.Biochem Biophys ResCommun.1997 Aug 18;237(2):225-229.
[13]夏群,冯远明,金鸿宾,等.脉冲电磁场对骨折愈合及钙磷代谢的影响.中国骨伤杂志,1999,12(4):3-4.
[14]阮默,李主一,邹培,等.脉冲电磁场对山羊骨延长区骨矿化的 影响[J].中华物理医学与康复杂志.2000;22(6):363-365.
[15]Hartiq M,Joos U,Wiesmann HP.Capacitively coupled electric fields accelerate proliferation of osteblast-like primary cells and increase bone extracellular matrix formation in vitro.Eur Biophys J,2000;29(7):499-506.
[16]Inoue N,Ohnishi I,Chen D,et al.Effect of pulsed electromagnetic fields(PEMF)on late-phase osteomy gap healing in a canine tibial model.J Orthop Res.2002,Sep;20(5):1106-1114.
[17]Chang WH,Chen LT,Sun JS,et al.Effect of pulseburst electromagnetic field stimulation on osteoblast cell activities.Bioelectromagnetics,2004 Sep;25(6):457-465.
[18]许兆南,靳振环.应用永久磁场治疗3例骨折延迟愈合的初步观察[J].徐州医药卫生,1976,(1):24.
[19]Bassett CA,Mitchell SN,Gaston SR.Pusling electromagnetic field treatment in ununited fractures and failed arthrodeses.JAMA,1982 Feb 5;247(5):623-628.
[20]Gerland DE,Moses B,Salver W.long-term follow-up of fracture nonunious treated with PEMFs.Contemp Orthop,1991Mar;22(3):295-302.
[21]吴立东,范顺武,潘志军,等.脉冲电磁场治疗骨折53例[J].浙江医科大学学报,1994,23(5):216-217.
[22]陈惟,左德秀,丁建群,等.运用磁贴与关节松动术治疗趾骨骨折26例[J].中国康复,1997,12(2):20.
[23]Satter Sved A,Islam MS,Rabbani KS,et al.Pulsed electromagnetic fields for the treatment of bone fractures.Bangladesh Med Res Counc Bull,1999 Apr;25(1):6-10.
[24]葛京化,侯宝兴,郑培永.静磁场促进Colles骨折愈合的临床研究--附75例病例分析.上海中医药杂志.2002,9:33-34.
[25]叶日乔,黄有荣,王大伟等.脉冲电磁刺激临床应用研究.中医正骨.2004,16(11):11-12.
[26]阳波,杨静.脉冲磁场对中老年女性股骨颈骨折髋关节功能恢复及早期血清炎性因子的影响.创伤外科杂志.2006,8(5):461.
[27]Basett CA.The development and application of pulsed electromagnetic fields for ununited fractures and arthrodeses[J]Clin Hast Surg.1985,12(2):259.
[28]Grace KL,Revell WJ,Brookes M.The effects of pulsed electromagnetism on fresh fracture healing:osteochondral repair in the rat femoral groove[J].Orthopedics.1998,21(3):293.
[29]Landry PS,Sadasivan KK,Marlno AA,et al.Electromagnetic fields can affect osteogenesis by the rate of differentiation[J].Clin Orthop.1997,338:362.
[30]Torrlcelli P,FiniM,Gia varei G,et al.In vitro osteoinduction of demineralized bone[J].Artif Cells Blood Immbil Biotechnol,1998,26(3):39.
[31]Bersani F,Ryaby iT,Magee FP,et al.Intramembrane protein distribution in cell culture is affected by 50HZ pulsed magnetic fields[J].Bioelectromagnetics,1997,18(7):463.
[32]Heermeier K,Spanner M,Trager J,et al,Effects of extremely low frequency electromagnetic field(EMF)oncollagen type Ⅰ mRNA expression and extracelluar matrix synthesis of human osteoblsatic cells[J].Bioelectromagnetic,1998,19(4):222.
[33]Bodamyli T,Bhatt B,Hughes FJ,et al.Pulsed electromagnetic fields simultaneously induce osteogenesis and upregulat trascriptioan of bone morphogenetic protein 2 and 4 in rat osteoblast in vitro[J].Biochem Biophys Res Commun.1998,250(2):458.
[34]王万春,黄兆民.磁性接骨板对骨折愈合的实验研究.中华理疗杂志,1995,18(2):71-73.
[35]朱天岳.微量直流电对家兔实验性骨折愈合的影响.中华骨科杂志,1984,4(6):363-366.
[36]周万松磁场对微循环影响的国内研究概况中华理疗杂志,1994,17(3):167-169.
[37]王学理,马定祥.脉动磁场促进下颌骨新鲜骨折愈合的实验研究.中华生物磁学.1990,4(3 4):83-87.
[38]Wilson DH,Jaqudesh P.Exiperimental regeneration in peripheral nerves and the spinal cord in laboratory animals exposed to a pulsed electromagnetic field.Paorapleqia.1976,14(1):12-20.
[39]Meissl G.Nerve regeneration by means of the effect of an intermitt electromagnetic field:Experimental study on a rabbit.Handchirurqie.1979,11(1):31-35.
[40]Raji AR,Bowwden RE.Effects of high-peak pulsed electromagnetic field on the degeneration and regeneration of the common peroneal nerve in rats.J Bone Joint Surg Br.1983,65(4):478-492.
[41]Orgel MG,O'Brien WJ,Murray HM.Pulsing electromagnetic field therapy in nerve regeneration:an experimental study in the cat.Plast Reconstr Surg.1984,73(2):173-183.
[42]Zienowicz RJ,Thomas BA,Kurtz WH,et al.Amultivariate approach to the treatment of peripheral nerve transection injury:the role of electromagnetic field therapy.Plast reconstr Surg.1991,87(1)122-129.
[43]郭瑞华,黄赛清,贾鸿妹,等.脉冲电磁场促进周围神经再生的实验研究[J].中国康复医学杂志.1999,14(3):1Ⅰ2-114.
[44]周田华,李主一,周中英,等.电磁场促进周围神经再生作用机制探讨[J].中华理疗杂志,2001.24(4):209-211.
[45]郭风劲,李新志,许涛,等.磁刺激对脊髓神经组织损伤的早期保护作用.中国康复.2001,16(1):4-6.
[46]胡永善,白玉龙,林伟平,等.经颅磁电刺激促进周围神经再生的电生理学研究.中国康复医学杂志.2001,16(4):216-218.
[47]白玉龙,胡永善,林伟平,等.经颅磁电刺激和局部电刺激促进周围神经再生的比较研究.中国康复医学杂志,2003,18(2):88-90.
[48]Kim SS,Shin HJ,Eom DW,et al.Enhanced expression of neuronal nitric oxide synthase and phospholipase C-gamma 1 in regenerating murine neuronal cells by electromagnetic field.Exp Mol Med.2002,34(1):53-59.
[49]Poirrier AL,Nyssen Y,Scholtes F,et al.Repetitive transcranial magnetic stimulation improves open field locomotor recovery after low but not high thoracic spinal cord compression-injury in adult rats.J Neurosci Res.2004,75(27):253-261.
[50]许涛,郭风劲,郭铁成,等.磁刺激对大鼠离体神经干细胞生长和分化的影响.中华物理医学与康复杂志.2005,27(3):129-132.
[51]许涛,饶耀剑,郭风劲,等.磁刺激对大鼠神经干细胞CDK5基因表达的影响.中国康复.2006,21(5):294-296.
[52]Bervar M.Effect of weak,interrupted sinusoidal low frequency magnetic field on neural regeneration in rats:functional evaluation.Bioelectromagnetics.2005,26(5):351-356.
[53]Mert T,Gunay I,Gocmen C,et al.Regenerative effects of pulsed magnetic field on injured peripheral nerves.Altern Ther Health Med.2006,12(5):42-49.
[54]Guven M,Gunay I,Ozqunen K,et al.Effect of pulsed magnetic field on regenerating rat sciatic nerve:an in-vitro electrophysiologic study.Znt J Neurosci.2005,115(6):881- 892.
[55]Kelleher MO,Al-Abri RK,Lenihan DV,et al.Use of a ststic magnetic field to promote recovery after peripheralnerve injury.J Neurosurg.2006,105(4):610-615.
[56]康庆林.田万中,潘希贵,等.低频电磁场促进手部Ⅱ区屈指肌腱愈合[J].中华理疗杂志,1998,21(6):355-356.
[57]Strauch B,Patel MK,Rosen DJ,et al.Pusled magnetic field therapy increases tensile strength in a rat:A chilles'tendon repair model.J Hand Surg[Am].2006,31(7):1131-1135.
[58]Sta Iqlesia DD,Cragoe EJ Jr,Vanable JW Jr.Electric field strength and epithelization in the newt(Notophthalmus viridescens).J Exp Zool.1996,274(1):56-62.
[59]Zhao M,Aqins-Fernandez A,Forrester JV,et al.Directed migration of corneal epithelial sheets in physiological electric field Invest Ophthalmol Vis Sci.1996,37(13)2548-2558.
[60]Fang KS,Farboud B,Nuccitelli R,et al.Migration of human keratinocytes in electric fields requires growth factors and extracellular calcium.J Invest Dermatol.1998,111(5):751-756.
[61]Pullar CE,Isseroff RR,Nuccitelli R.Cylic AMP-dependent protein kinase A plays a role in the directed migration of human keratinocytes in a De dectric field,Cell Motil Cytoskeleton 2001,50(4):207-217.
[62]Li X,Kolega J.Effects of direct current electric fields on cell migration and actin filament distribution in bovine vascular endothelial cells.J Vase Res.2002,39(5):391-404.
[63]Zhao M,Song B,Pu J,et al.Electrical signals control wound heling through phosphatidylinositol-3-OH kinase-gamma and PTEN.Nature.2006,442(7101):457-460.
[64]肖忠文,邓秀明,郑高辉,等.磁场对小白鼠实验性创伤愈合作用的观察.中华物理医学杂志,1984,6(4):231.
[65]常汉英,于国,李晖,等.磁场对瘢痕组织的影响[J].中华理疗杂志,1997.20(1):19-22.
[66]亢国英,陈金荣,刘乃国,等.磁疗对烫伤大鼠皮肤痛阈和创面愈合的影响[J].宾州医学院学报,1998,21(5):430-431.
[67]王明华,孔祥鸣,张小云,等.强磁场对小鼠创伤修复和瘢痕粘连的影响[J].中华物理医学与康复杂志,2000,22(6):359-362.
[68]郝希平,宋晓伟,雷万军,等.旋磁对小鼠创伤修复和自由基的影响[J].河南科技大学学报(医学版).2004,22(1):55-56.
[69]金容成,来华,李维礼.磁场对动物创面影响的实验观察.中华物理医学杂志.1982,4(4):20.
[70]熊伟,肖萍.稀土钴永磁贴敷对伤口愈合的实验报告[J].中华生物磁学.1989,3(1-2):104.
[71]杨翔云,于恩华,车世铭.新型永磁体对术后创口愈合的作用.中华生物磁学.1989,3(1-2):116.
[72]粱应权,袁云华,张文玉,等.静磁场对慢性小腿溃疡的初步疗效及组织病理学观察.中华理疗杂志,1994,17(1):24.
[73]罗二平,申广浩,周龙甫,等.稳恒0.2T磁场对兔创伤修复的影响[J].第四军医大学学报.2004,25(13):1191-1193.
[74]王军学,罗二平,申广浩,等.恒磁场对临床术后伤口愈合的影响[J].第四军医大学学报.2006,27(8):754-756.
[75]Bertolino G,de Freitas Braga A,de Oliveira Lima do Couto Rosa K,et al.Macroscopic and histological effects of magnetic field exposition in the process of tissue reparation in Wistar rats.Arch Dermatol Res.2006,298(3):121-126.
[2]罗二平,申广浩,周龙甫,等.稳恒0.2T磁场对兔创伤修复的影响[J].第四军医大学学报.2004,25(13):1191-1193.
[3]王军学,罗二平,申广浩,等.恒磁场对临床术后伤口愈合的影响[J].第四军医大学学报.2006,27(8):754-756.
[4]Cribbs RK,Luguetts MH,Besner GE.Acceleration of pardal thickness bum wound healing with topical application of heparin-binding EGF-like growth factor(HB-EGF).J Bwn Care Rehatic.1998;19:95.
[5]李爱冰,袁先厚,陈新军.VEGF bFGF在血管网织细胞瘤中的表达.中国临床神经外科杂志.2000,5(4):229-231.
[6]Leung DW,Cachines G,Kuang WJ,et al.Vascular endothelial growth factor issecreted angiogenic mitogen[J].Science,1989,246:1306.
[7]Terman BI,DougherV,Ermazen M,et al.Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor[J].Biochem Biophys Res Commun,1992,187:1579.
[8]Okano h,Onmori R,Tomita N,et al.Effects of a moderate- intensity static magnetic field on VEGF-A stimulsted endothelial capillary tuble formation in vitro.Bioelectromagnetics,2006 Dec;27(8):6284FI0.
[9]Dvorak HK.Angiogenesis:update 2005[J].J Thmmb Haemost,2005,3(8):1835-1842.
[10]彭湃,郭树忠,韩岩,等.VEGF对创伤组织中KDR,bFGF和PDGF mRNA 表达的影响.中国美容医学,2004,13(3):266-268.
[11]许伟榕,王莉,赵涵芳,等.人血管内皮生长因子基因工程生物膜愈合大鼠皮肤创伤的初步研究.外科理论与实践,2006,11(3):244-247.
[12]赵雷,王莉,姜叙诚,等.hVEGF基因工程生物膜对全层皮肤缺损创面愈合的影响上海交通大学学报(医学版),2006,26(9):1015-1018.
[13]彭瑞云,王德文,高亚兵,等.大鼠皮肤伤口内VEGF基因表达的定量病理研究.中国体视学与图像分析.2001,6(2):95-98.
[14]彭瑞云,高亚兵,熊程奇,等.正常和放射伤口血管再生中VEGF基因的表达及其意义.细胞与分子免疫学杂志.2001,17(5):410-412.
[15]Dvorak HF,Brown LF,Detmar M,et al.Vascular permeability factOr/vascular endothelial growth factor.Microvascular hyperpermeability,and angiogenesis.Am J Pathol.1995;146(5):1029.
[16]杨述华,周迎春,杨操,等.血管内皮生长因子基因转染促进伤口 愈合的实验研究.中华骨科杂志,2001,21(5):301-304.
[17]谢卫国,Werner Lindenmaier,Stefan Gryzybowsk,等.血管内皮生长因子基因修饰对裸鼠移植皮肤替代物的影响.中华烧伤杂志,2005,21(3):203-206.
[18]刘亮,刘旭胜.巨噬细胞在创伤愈合血管生成中作用的研究进展.中华烧伤杂志.2004,20(4):249-251.
[19]陈欣,副岛一孝,野崎斡弘,等.成纤维细胞移植促进人工真皮内血管新生的研究.中国修复重建外科杂志.2004,18(3):205-208.
[1]Bassett CA.Tibial fracture healing due to constant electrieal field.J.Bone Joint Sury,1977-53(A):1655.
[2]Dehass WG.The effects of low frequency magnetic fields on the healing of the rabbit.Clin Orthop.1979.145:245.
[3]Banal.The classic fudumenlal aspects of fracture.Clin Orthop.1977,124-125.
[4]刘海起,朱显,赵士林,等.磁与自然铜促进骨折愈合的初步实验研究.中华外科杂志,1983,21(1):1-4.
[5]May HU,Nippel FJ,Hansjurgens A,et al.Acceleration of ossification by means of interferential current.Prog Clin Biol Res.1985;187:469-478.
[6]Brighton CT,Hozack WJ,Brager MD,et al.Fracture healing in the rabbit fibula when subjected to various capacitively coupled electrical fields.J Orthop Res.1985;3(3):331-340.
[7]李仁杰,柳用墨,王崇武.旋磁场促进骨折愈合的初步实验研究[J].中华骨科杂志.1988,8(6):445.
[8]崔保义,钟锐球.磁场促进“骨不连”和“延迟愈合”性骨折愈合研究[J].中国磁学磁性材料杂志,1990,11(18):153.
[9]崔保义,钟锐球.磁场促进家兔骨折愈合部分病理学研究[J].中国磁性材料杂志,1993,12(27):747.
[10]续力民,康树林,李海亭,等.体内磁场促进骨折愈合的实验研究[J].北京军区医药,1994(5):329-331.
[11]Deibert MC,Mcleod BR,Smith SD,et al.Ion responance electromagnetic field stimulation of fracture healing in rabbits with a fibular ostecomy.J Orthop Res.1994Nov;12(6):878-885.
[12]Zhuang H,Wang W,Seldes RM,et al.Electrical stimulation induces the level of TGF-β1 mRNA in osteoblaetic cells by a mechanism involving calcium/calmodulin paathway.Biochem Biophys ResCommun.1997 Aug 18;237(2):225-229.
[13]夏群,冯远明,金鸿宾,等.脉冲电磁场对骨折愈合及钙磷代谢的影响.中国骨伤杂志,1999,12(4):3-4.
[14]阮默,李主一,邹培,等.脉冲电磁场对山羊骨延长区骨矿化的 影响[J].中华物理医学与康复杂志.2000;22(6):363-365.
[15]Hartiq M,Joos U,Wiesmann HP.Capacitively coupled electric fields accelerate proliferation of osteblast-like primary cells and increase bone extracellular matrix formation in vitro.Eur Biophys J,2000;29(7):499-506.
[16]Inoue N,Ohnishi I,Chen D,et al.Effect of pulsed electromagnetic fields(PEMF)on late-phase osteomy gap healing in a canine tibial model.J Orthop Res.2002,Sep;20(5):1106-1114.
[17]Chang WH,Chen LT,Sun JS,et al.Effect of pulseburst electromagnetic field stimulation on osteoblast cell activities.Bioelectromagnetics,2004 Sep;25(6):457-465.
[18]许兆南,靳振环.应用永久磁场治疗3例骨折延迟愈合的初步观察[J].徐州医药卫生,1976,(1):24.
[19]Bassett CA,Mitchell SN,Gaston SR.Pusling electromagnetic field treatment in ununited fractures and failed arthrodeses.JAMA,1982 Feb 5;247(5):623-628.
[20]Gerland DE,Moses B,Salver W.long-term follow-up of fracture nonunious treated with PEMFs.Contemp Orthop,1991Mar;22(3):295-302.
[21]吴立东,范顺武,潘志军,等.脉冲电磁场治疗骨折53例[J].浙江医科大学学报,1994,23(5):216-217.
[22]陈惟,左德秀,丁建群,等.运用磁贴与关节松动术治疗趾骨骨折26例[J].中国康复,1997,12(2):20.
[23]Satter Sved A,Islam MS,Rabbani KS,et al.Pulsed electromagnetic fields for the treatment of bone fractures.Bangladesh Med Res Counc Bull,1999 Apr;25(1):6-10.
[24]葛京化,侯宝兴,郑培永.静磁场促进Colles骨折愈合的临床研究--附75例病例分析.上海中医药杂志.2002,9:33-34.
[25]叶日乔,黄有荣,王大伟等.脉冲电磁刺激临床应用研究.中医正骨.2004,16(11):11-12.
[26]阳波,杨静.脉冲磁场对中老年女性股骨颈骨折髋关节功能恢复及早期血清炎性因子的影响.创伤外科杂志.2006,8(5):461.
[27]Basett CA.The development and application of pulsed electromagnetic fields for ununited fractures and arthrodeses[J]Clin Hast Surg.1985,12(2):259.
[28]Grace KL,Revell WJ,Brookes M.The effects of pulsed electromagnetism on fresh fracture healing:osteochondral repair in the rat femoral groove[J].Orthopedics.1998,21(3):293.
[29]Landry PS,Sadasivan KK,Marlno AA,et al.Electromagnetic fields can affect osteogenesis by the rate of differentiation[J].Clin Orthop.1997,338:362.
[30]Torrlcelli P,FiniM,Gia varei G,et al.In vitro osteoinduction of demineralized bone[J].Artif Cells Blood Immbil Biotechnol,1998,26(3):39.
[31]Bersani F,Ryaby iT,Magee FP,et al.Intramembrane protein distribution in cell culture is affected by 50HZ pulsed magnetic fields[J].Bioelectromagnetics,1997,18(7):463.
[32]Heermeier K,Spanner M,Trager J,et al,Effects of extremely low frequency electromagnetic field(EMF)oncollagen type Ⅰ mRNA expression and extracelluar matrix synthesis of human osteoblsatic cells[J].Bioelectromagnetic,1998,19(4):222.
[33]Bodamyli T,Bhatt B,Hughes FJ,et al.Pulsed electromagnetic fields simultaneously induce osteogenesis and upregulat trascriptioan of bone morphogenetic protein 2 and 4 in rat osteoblast in vitro[J].Biochem Biophys Res Commun.1998,250(2):458.
[34]王万春,黄兆民.磁性接骨板对骨折愈合的实验研究.中华理疗杂志,1995,18(2):71-73.
[35]朱天岳.微量直流电对家兔实验性骨折愈合的影响.中华骨科杂志,1984,4(6):363-366.
[36]周万松磁场对微循环影响的国内研究概况中华理疗杂志,1994,17(3):167-169.
[37]王学理,马定祥.脉动磁场促进下颌骨新鲜骨折愈合的实验研究.中华生物磁学.1990,4(3 4):83-87.
[38]Wilson DH,Jaqudesh P.Exiperimental regeneration in peripheral nerves and the spinal cord in laboratory animals exposed to a pulsed electromagnetic field.Paorapleqia.1976,14(1):12-20.
[39]Meissl G.Nerve regeneration by means of the effect of an intermitt electromagnetic field:Experimental study on a rabbit.Handchirurqie.1979,11(1):31-35.
[40]Raji AR,Bowwden RE.Effects of high-peak pulsed electromagnetic field on the degeneration and regeneration of the common peroneal nerve in rats.J Bone Joint Surg Br.1983,65(4):478-492.
[41]Orgel MG,O'Brien WJ,Murray HM.Pulsing electromagnetic field therapy in nerve regeneration:an experimental study in the cat.Plast Reconstr Surg.1984,73(2):173-183.
[42]Zienowicz RJ,Thomas BA,Kurtz WH,et al.Amultivariate approach to the treatment of peripheral nerve transection injury:the role of electromagnetic field therapy.Plast reconstr Surg.1991,87(1)122-129.
[43]郭瑞华,黄赛清,贾鸿妹,等.脉冲电磁场促进周围神经再生的实验研究[J].中国康复医学杂志.1999,14(3):1Ⅰ2-114.
[44]周田华,李主一,周中英,等.电磁场促进周围神经再生作用机制探讨[J].中华理疗杂志,2001.24(4):209-211.
[45]郭风劲,李新志,许涛,等.磁刺激对脊髓神经组织损伤的早期保护作用.中国康复.2001,16(1):4-6.
[46]胡永善,白玉龙,林伟平,等.经颅磁电刺激促进周围神经再生的电生理学研究.中国康复医学杂志.2001,16(4):216-218.
[47]白玉龙,胡永善,林伟平,等.经颅磁电刺激和局部电刺激促进周围神经再生的比较研究.中国康复医学杂志,2003,18(2):88-90.
[48]Kim SS,Shin HJ,Eom DW,et al.Enhanced expression of neuronal nitric oxide synthase and phospholipase C-gamma 1 in regenerating murine neuronal cells by electromagnetic field.Exp Mol Med.2002,34(1):53-59.
[49]Poirrier AL,Nyssen Y,Scholtes F,et al.Repetitive transcranial magnetic stimulation improves open field locomotor recovery after low but not high thoracic spinal cord compression-injury in adult rats.J Neurosci Res.2004,75(27):253-261.
[50]许涛,郭风劲,郭铁成,等.磁刺激对大鼠离体神经干细胞生长和分化的影响.中华物理医学与康复杂志.2005,27(3):129-132.
[51]许涛,饶耀剑,郭风劲,等.磁刺激对大鼠神经干细胞CDK5基因表达的影响.中国康复.2006,21(5):294-296.
[52]Bervar M.Effect of weak,interrupted sinusoidal low frequency magnetic field on neural regeneration in rats:functional evaluation.Bioelectromagnetics.2005,26(5):351-356.
[53]Mert T,Gunay I,Gocmen C,et al.Regenerative effects of pulsed magnetic field on injured peripheral nerves.Altern Ther Health Med.2006,12(5):42-49.
[54]Guven M,Gunay I,Ozqunen K,et al.Effect of pulsed magnetic field on regenerating rat sciatic nerve:an in-vitro electrophysiologic study.Znt J Neurosci.2005,115(6):881- 892.
[55]Kelleher MO,Al-Abri RK,Lenihan DV,et al.Use of a ststic magnetic field to promote recovery after peripheralnerve injury.J Neurosurg.2006,105(4):610-615.
[56]康庆林.田万中,潘希贵,等.低频电磁场促进手部Ⅱ区屈指肌腱愈合[J].中华理疗杂志,1998,21(6):355-356.
[57]Strauch B,Patel MK,Rosen DJ,et al.Pusled magnetic field therapy increases tensile strength in a rat:A chilles'tendon repair model.J Hand Surg[Am].2006,31(7):1131-1135.
[58]Sta Iqlesia DD,Cragoe EJ Jr,Vanable JW Jr.Electric field strength and epithelization in the newt(Notophthalmus viridescens).J Exp Zool.1996,274(1):56-62.
[59]Zhao M,Aqins-Fernandez A,Forrester JV,et al.Directed migration of corneal epithelial sheets in physiological electric field Invest Ophthalmol Vis Sci.1996,37(13)2548-2558.
[60]Fang KS,Farboud B,Nuccitelli R,et al.Migration of human keratinocytes in electric fields requires growth factors and extracellular calcium.J Invest Dermatol.1998,111(5):751-756.
[61]Pullar CE,Isseroff RR,Nuccitelli R.Cylic AMP-dependent protein kinase A plays a role in the directed migration of human keratinocytes in a De dectric field,Cell Motil Cytoskeleton 2001,50(4):207-217.
[62]Li X,Kolega J.Effects of direct current electric fields on cell migration and actin filament distribution in bovine vascular endothelial cells.J Vase Res.2002,39(5):391-404.
[63]Zhao M,Song B,Pu J,et al.Electrical signals control wound heling through phosphatidylinositol-3-OH kinase-gamma and PTEN.Nature.2006,442(7101):457-460.
[64]肖忠文,邓秀明,郑高辉,等.磁场对小白鼠实验性创伤愈合作用的观察.中华物理医学杂志,1984,6(4):231.
[65]常汉英,于国,李晖,等.磁场对瘢痕组织的影响[J].中华理疗杂志,1997.20(1):19-22.
[66]亢国英,陈金荣,刘乃国,等.磁疗对烫伤大鼠皮肤痛阈和创面愈合的影响[J].宾州医学院学报,1998,21(5):430-431.
[67]王明华,孔祥鸣,张小云,等.强磁场对小鼠创伤修复和瘢痕粘连的影响[J].中华物理医学与康复杂志,2000,22(6):359-362.
[68]郝希平,宋晓伟,雷万军,等.旋磁对小鼠创伤修复和自由基的影响[J].河南科技大学学报(医学版).2004,22(1):55-56.
[69]金容成,来华,李维礼.磁场对动物创面影响的实验观察.中华物理医学杂志.1982,4(4):20.
[70]熊伟,肖萍.稀土钴永磁贴敷对伤口愈合的实验报告[J].中华生物磁学.1989,3(1-2):104.
[71]杨翔云,于恩华,车世铭.新型永磁体对术后创口愈合的作用.中华生物磁学.1989,3(1-2):116.
[72]粱应权,袁云华,张文玉,等.静磁场对慢性小腿溃疡的初步疗效及组织病理学观察.中华理疗杂志,1994,17(1):24.
[73]罗二平,申广浩,周龙甫,等.稳恒0.2T磁场对兔创伤修复的影响[J].第四军医大学学报.2004,25(13):1191-1193.
[74]王军学,罗二平,申广浩,等.恒磁场对临床术后伤口愈合的影响[J].第四军医大学学报.2006,27(8):754-756.
[75]Bertolino G,de Freitas Braga A,de Oliveira Lima do Couto Rosa K,et al.Macroscopic and histological effects of magnetic field exposition in the process of tissue reparation in Wistar rats.Arch Dermatol Res.2006,298(3):121-126.