幼龄茶树在干旱胁迫下基因表达差异分析
|
摘要
茶树(Camellia sinensis(L.) O. Kuntze)是我国重要的经济作物之一,不仅栽培历史悠久,而且分布广泛。已有研究表明,我国西南部是茶树的起源中心,孕育了丰富的茶树种质资源,为优良品种的选育奠定了优良的基础。干旱是限制世界作物生产的主要环境因子之一,全球干旱、半干旱地区约占土地总面积的36%,占耕地面积的43%。我国干旱和半干旱地区面积约占全国土地面积的1/2,同时,随着全球温室效应的增强,对抗旱品种的选育将是未来品种的选育的一个重要方面。
传统的茶树品种选育主要是通过系统选育和单株选育,但是有遗传背景复杂、遗传不稳定、选育时间较长、选育盲目性较大等缺陷,同时抗旱指标的选择多是以环境性状为主。分子育种直接在分子水平选育,通过对遗传物质DNA的筛选进行品种选育,能有效避免上述缺点。DD-PCR(差异显示PCR技术,或DDRT-PCR)有操作简便、灵敏性良好和效率较高的优点,本研究首次将技术成熟的差异显示PCR技术应用于茶树干旱环境下的差异表达基因的筛选。本实验意图将在干旱环境下有表达变化的基因片段作为以后抗旱品种研究和选育的一个筛选指标,对干旱品种的选育做出一定的贡献。通过实验得到了以下实验结果：
1、幼龄茶苗利用试剂盒提取RNA的最佳部位是嫩叶和幼根,因为积累的次生产物少,对提取到的RNA污染少,但根部RNA保存较困难,降解较快,说明应用该试剂盒提取的RNA中有其他杂质,影响根部RNA的保存。
2、PCR反应时用Mixmixture的扩增效果要好于传统的Taq酶体系；电泳条件是120mv,电泳1h左右；电泳所用凝胶浓度约为1.5%分离效果较好；取材部位为嫩叶时,退火温度为45℃时,扩增条带的假阳性较低,条带的重复性较好,取材部位为幼根时,根据处理时间,应适当降低退火温度；PCR反应参数以普通PCR反应参数为对照即可。
3、经过PCR反应后,发现条带有上调表达、下调表达、常量表达三种表达量的变化；发现部分条带对锚定引物有一定的兼并性：通过引物筛选,确定了进行不同研究所需要的引物组合,以幼根为材料进行试验,需要重新确定胁迫剂浓度和胁迫处理时间。
4、根据测序结果可以看出,茶苗在干旱胁迫处理下,有很多基因条带表达产生变化,部分条带的变化趋势和有研究者报道的酶活变化趋势相近。
5、确定应用DDRT-PCR技术在茶树抗旱分子机制的探讨中是可行的,在所选择的茶树品种福鼎大白茶幼苗上取得的较好的实验效果,可以应用该方法对其它茶树品种及不同发育期茶树品种进行相关研究。
Tea plant (Camellia sinensis (L.) O. Kuntze) is one of the important economic plants in China, which had long planting history and distributes widely. It has been proved that Southwest of China is the origin of tea plant, having rich of tea resources and good foundation for breeding. Drought is the main environmental limit factors in the world's crop production, one of the world's arid and semi-arid regions account for about 36% of the total land area, accounting for 43% of arable land. China's arid and semi-arid land area accounting for about 1/2, while, with the enhancement of Greenhouse effect, the of drought-resistant varieties breeding will be an important aspect in the future. The traditiong way of tea breeding is maine system breeding and plant breeding,but there are some drawback like genetic backgroud complex,breeding instability,needing long time and other defects,while the drought indicators are mostly based on environmental characteristics. Molecular breeding can effectively avoid these problems for it directly screening genetic material DNA. DD-PCR (differential display PCR, or DDRT-PCR) have advantages of simple,high sensitivity and efficiency.It is first time that study gene differentially expressed under arid environment.This experiment try to screen different exprement gene to drought-resistant index,intended to make some contribution to drought variety, and got results as followed:
1. The best part extracting RNA from young tea using kit is leaves and new roots,because its have less secondary products to pollution of the extracted RNA.But RNA from roots difficult to preservation as the same environment,rapid degradation,illustrating the exracting RNA with kit had other impurities affect the preservation of root RNA.
2.Amplification reaction with Mixmixture better than conventional Taq enzyme system in PCR; gel electrophoresis is 120mv;electrophoresis time about 1h;separation gel concentra-tion about1.5%;experimentparts part is leaves;the annealing temperature 45℃,when meeting these codition getting better result. When experiments parts is roots,we need put down.Annealing temperature.PCR reaction parameters is like ordinary PCR reaction.
3.There are three changes afer PCR:up expression,down expression and constant expression. Some bands have mergers on anchor primers.Identified anchor primers and random premers combinations after screening.Need to determine the stress time when experiment part is young roots.
4.There are many gene bands expression changed after drought stress from sequencing results,and some bands changed trend similar other researchers reported.
5.Making sure this method and technique is feasible in studying anti-drought molecular nechanism on the tea,for example,we achieved good experiments results in FuDing seeding,so the method can be applied to research other tea varieties and different breeding statement of tea.
传统的茶树品种选育主要是通过系统选育和单株选育,但是有遗传背景复杂、遗传不稳定、选育时间较长、选育盲目性较大等缺陷,同时抗旱指标的选择多是以环境性状为主。分子育种直接在分子水平选育,通过对遗传物质DNA的筛选进行品种选育,能有效避免上述缺点。DD-PCR(差异显示PCR技术,或DDRT-PCR)有操作简便、灵敏性良好和效率较高的优点,本研究首次将技术成熟的差异显示PCR技术应用于茶树干旱环境下的差异表达基因的筛选。本实验意图将在干旱环境下有表达变化的基因片段作为以后抗旱品种研究和选育的一个筛选指标,对干旱品种的选育做出一定的贡献。通过实验得到了以下实验结果：
1、幼龄茶苗利用试剂盒提取RNA的最佳部位是嫩叶和幼根,因为积累的次生产物少,对提取到的RNA污染少,但根部RNA保存较困难,降解较快,说明应用该试剂盒提取的RNA中有其他杂质,影响根部RNA的保存。
2、PCR反应时用Mixmixture的扩增效果要好于传统的Taq酶体系；电泳条件是120mv,电泳1h左右；电泳所用凝胶浓度约为1.5%分离效果较好；取材部位为嫩叶时,退火温度为45℃时,扩增条带的假阳性较低,条带的重复性较好,取材部位为幼根时,根据处理时间,应适当降低退火温度；PCR反应参数以普通PCR反应参数为对照即可。
3、经过PCR反应后,发现条带有上调表达、下调表达、常量表达三种表达量的变化；发现部分条带对锚定引物有一定的兼并性：通过引物筛选,确定了进行不同研究所需要的引物组合,以幼根为材料进行试验,需要重新确定胁迫剂浓度和胁迫处理时间。
4、根据测序结果可以看出,茶苗在干旱胁迫处理下,有很多基因条带表达产生变化,部分条带的变化趋势和有研究者报道的酶活变化趋势相近。
5、确定应用DDRT-PCR技术在茶树抗旱分子机制的探讨中是可行的,在所选择的茶树品种福鼎大白茶幼苗上取得的较好的实验效果,可以应用该方法对其它茶树品种及不同发育期茶树品种进行相关研究。
Tea plant (Camellia sinensis (L.) O. Kuntze) is one of the important economic plants in China, which had long planting history and distributes widely. It has been proved that Southwest of China is the origin of tea plant, having rich of tea resources and good foundation for breeding. Drought is the main environmental limit factors in the world's crop production, one of the world's arid and semi-arid regions account for about 36% of the total land area, accounting for 43% of arable land. China's arid and semi-arid land area accounting for about 1/2, while, with the enhancement of Greenhouse effect, the of drought-resistant varieties breeding will be an important aspect in the future. The traditiong way of tea breeding is maine system breeding and plant breeding,but there are some drawback like genetic backgroud complex,breeding instability,needing long time and other defects,while the drought indicators are mostly based on environmental characteristics. Molecular breeding can effectively avoid these problems for it directly screening genetic material DNA. DD-PCR (differential display PCR, or DDRT-PCR) have advantages of simple,high sensitivity and efficiency.It is first time that study gene differentially expressed under arid environment.This experiment try to screen different exprement gene to drought-resistant index,intended to make some contribution to drought variety, and got results as followed:
1. The best part extracting RNA from young tea using kit is leaves and new roots,because its have less secondary products to pollution of the extracted RNA.But RNA from roots difficult to preservation as the same environment,rapid degradation,illustrating the exracting RNA with kit had other impurities affect the preservation of root RNA.
2.Amplification reaction with Mixmixture better than conventional Taq enzyme system in PCR; gel electrophoresis is 120mv;electrophoresis time about 1h;separation gel concentra-tion about1.5%;experimentparts part is leaves;the annealing temperature 45℃,when meeting these codition getting better result. When experiments parts is roots,we need put down.Annealing temperature.PCR reaction parameters is like ordinary PCR reaction.
3.There are three changes afer PCR:up expression,down expression and constant expression. Some bands have mergers on anchor primers.Identified anchor primers and random premers combinations after screening.Need to determine the stress time when experiment part is young roots.
4.There are many gene bands expression changed after drought stress from sequencing results,and some bands changed trend similar other researchers reported.
5.Making sure this method and technique is feasible in studying anti-drought molecular nechanism on the tea,for example,we achieved good experiments results in FuDing seeding,so the method can be applied to research other tea varieties and different breeding statement of tea.
引文
[1]陈善福,舒庆尧.植物耐干旱胁迫的生物学机理及其基因工程研究进展[J].植物学通报.1999,16(5)：555-560
[2]石凤翎,郭晓霞,李红.扁蓿豆抗旱形态解剖结构观察与分析[J].干旱地区农业研究,2005,23(2)：115-118
[3]路贵和,黄淮海地区不同抗旱类型大豆种质资源根系特征及抗旱性研究[J].山西农业科学,2000,28(2)：37.-40
[4]曹雄,张小虎,任小俊.灰色理论在绿豆抗旱育种中的应用[J].国外农学-杂粮作物,1999,9(3)：19-21
[5]Carlos German Munoz-Perea, Henry Terdn, Richard G. Allen, James L. Wright,et.al. Selection for Drought Resistance in Dry Bean Landraces and Cultivars[J].Crop Science,2006,46:2111-2120
[6]Shree P. Singh. Drought Resistance in the Race Durango Dry Bean Landraces and Cultivars[J]. Agronomy Journal,2007,99:1219-1225
[7]毛淑蕊,李墨霞,万勇善,等,渗透胁迫对花生不定根再生及生长特性的影响[J].山东农业大学学报(自然科学版),2009,40(3)：329-33
[8]王育红,姚宇卿,吕军杰,等.花生抗旱指标研究初报[J].干旱地区农业研究,2002,20(3)：89-92
[9]P. Songsri, S. Jogloy, T. Kesmala,et.al. Heritability of Drought Resistance Traits and Correlation of Drought Resistance and Agronomic Traits in Peanut[J].Crop Science,2008,48:2245-2253
[10]Kpoghomou B K. Screening for drought tolerance soybean germination and its relationship to seedling response[J] Journal Agronomy & Crop Science,1990,164:153-159
[11]安永平,强爱玲,张媛媛,等.渗透胁迫下水稻种子萌发特性及抗旱性鉴定指标研究[J].植物遗传资源学报,2006,7(4)：421-426
[12]Jerome Bernier,Gary N Atlin,Rachid Serraj,et.al. Review:Breeding upland rice for drought resistance[J]. Journal of the Science of Food and Agriculture.2008,88:927-939
[13]Bing Yue, Lizhong Xiong, Weiya Xue,et.al. Genetic analysis for drought resistance of rice at re-productive stage in field with different types of soil[J]. Theoretical and Applied Genetics,2005,111 (6): 1127-1136
[14]陈凤梅,程建峰,潘晓云,等.杂交稻抗旱性状的筛选研究[J].杂交水稻2001,16(4)：51-54
[15]Z. Peleg, T. Fahima, S. Abbo,et.al.Genetic diversity for drought resistance in wild emmer wheat wheat and its ecogeographical associations[J]. Plant, Cell and Environment.2005,28:176-191
[16]R.M. Trethowan, M. Reymolds. Drought ResistanceE:Genetic Approaches for Improveing Productivity Under Stress[J]. Wheat Production in Stressed Environmen-ts.2007:289-299
[17]刘桂茹,葛淑俊,王静华,等.PEG对小麦花药出愈率的影响[J].河北农业大学学报,2001,24(4)：25-27
[18]M.J. Foulkes, R. Sylvester-Bradley, R. Weightman,et.al. Identifying physiological traits associat-ed with improved drought resistance in winter wheat[J].Field Crops Research,2007,103:11-24
[19]王瑾,刘桂茹,杨学举.胁迫下小麦再生植株根系特性与抗旱性的关系[J].麦类作物学报,2006,26(3)：117-119
[20]张振平,孙世贤,张悦,等.玉米叶部形态指标与抗旱性的关系研究[J].玉米科学,2009,17(3)：68-70
[21]树宾,王有德,等.西北旱农区玉米抗旱性的相关研究[J].新疆农业科,2001,38(2)：58-59
[22]赵美玲.玉米各生育时期抗旱性鉴定指标的研究[J].中国农学通报2009,25(12)：66-68
[23]张雪妍,刘传亮,王俊娟.PEG胁迫方法评价棉花幼苗耐旱性研究[J].棉花学报,2007,19(3)：205-209
[24]Herv6 Cochard, S.Tete Barigah, Marc Kleinhentz,et.al. Is xylem cavitation resistance a relevant criterion for screening drought resistance among Prunus species?[J]. Journal of Plant Physiology,2008, 165:976-982
[25]胡向敏,云锦凤.针茅族3种多年生牧草苗期抗旱性的比较研究[J].牧草与饲料科学,2009,3(2)：18-21
[26]赵相勇,陈培燕,袁庆华,等.高羊茅苗期抗旱性的生理研究[J].西南农业报,2009,22(3)：621-624
[27]焦树英,李永强,沙依拉.沙尔合提.等.干旱胁迫对3种狼尾草种子萌发和幼苗生长的影响[J].西北植物学报,2009,29(2)：0308-0313
[28]李晚忱,付凤玲,袁佐清.玉米苗期耐旱性鉴定方法研究[J].西南农业学报,2001,14(3)：29-32
[29]孙祖东,陈怀珠,杨守臻.苗期干早胁迫对大豆生长发育的影响与耐早材料的筛选[J].厂西农业科学,2002,1：11-13
[30]董立峰,尹秀玲,等.小麦离体叶片室内自然干化失水率与品种抗旱性关系的研究[J].河北农业技术师范学院学报,1997,11(1)：50-54
[31]张春霞,谢惠民,王婧,等.小麦品种叶片水势与抗旱节水性的关系[J].麦类作物报,2009,29(3)：453-459
[32]杨琳,景继海,赵佰图.旱地小麦品种和自育高代品系资源抗旱性鉴定研[J].陕西农业科 学,2009,4：42-46
[33]郭数进,李贵全.大豆生理指标与抗旱性关系的研究[J].河南农业科学,2009,6：38-41
[34]郭数进,李贵全.干旱胁迫对不同大豆品系酶类变化的影响[J].大豆科学,2009,28(3)：445-449
[35]郭数进,李贵全.干旱胁迫下不同大豆品系生理指标变化与抗旱性关系的研究[J].中国种业,2009，6：38-40
[36]赵玲,马向丽,邓祥升,等.干旱胁迫对草地早熟禾抗旱生理的影响[J].草业与畜牧,2009,6：37-41
[37]刘云开,方宝华,庞冰,等.水稻品种节水抗旱适应性筛选研究[J].湖南农业科学,2009,(5)：24-26
[38]罗俊,林彦铨,张木清.甘蔗活性氧代谢对水分胁迫的响应[J].福建农业大学学报,2009,29(4)：405-410
[39]罗俊,林彦铨,吕建林,等.水分胁迫对甘蔗叶片光合性能的影响[J].中国农业科学,2000,33(4)：100-102
[40]薛立,薛晔,任向荣,等.PEG模拟干旱条件下尾叶桉和枫香苗木的生理响应[J].生态环境学报,2009,18(2)：614-620
[41]陈由强,朱锦懋,叶冰莹.水分胁迫对芒果(Mangifera indica L.)幼叶细胞活性氧伤害的影响[J].生命科学研究,2000,4(1)：60-64
[42]叶冰莹,陈由强,朱锦懋,等.水分胁迫对三种木麻黄小枝活性氧伤害的研究[J].福建师范大学学报(自然科学版),2000,16(1)：76-79
[43]陈静,邢菊香,李春红.绣球绣线菊和土庄绣线菊强的抗旱生理特性比较[J].内蒙古农业大学学报,2009,30(1)：295-298
[44]李磊,贾志清,宁虎森,等.水分胁迫下2种沙枣的抗旱性比较[J].林业科学研究,2009,22(3)：335-342
[45]荣红颖,张立全,杨凤萍,等.DREBIB基因在转基因小麦后代的稳定表达[J].分子植物育种,2009,7(3)：437-443
[46]王婧,谢惠民,张春霞,等.冬小麦幼苗期水分胁迫应答蛋白遗传分析及基因初步定位[J].麦类作物学报2009,29(3)：396-400
[47]杜丽璞,徐惠君,叶兴国,等.小麦转TPS基因植株的获得及其初步功能鉴定[J].麦类作物学报2007,27(3)：369-373
[48]徐重益.小麦抗旱相关基因TaPP2Ac/a的分离、定位与功能验证[D].中国农业科学院作物科学研究所,2007,7
[49]王静英,李永春,王潇,等.小麦TaLEA4基因的克隆和表达[J].麦类作物学报,2008,28(2)：183- 186
[50]Hong hong Hu,Mingqiu Dai,Jialing Yao.et.al. Overexpressing a NAM, ATAF, and CUC(NAC) Transcription factor enhances drought resistance and salt tolerance in rice[J]. PANS,2006,103(35): 1297-1292
[51]Yuemin Huang,Benze Xiao,Lizhong Xiong. Characterization of a stress responsive proteinase inhibitor gene with positive effect in improving drought resistance in rice[J]. Planta,2007,226:73-85
[52]Ben-Ze Xiao, Xi Chen, Cheng-Bin Xiang,et.al. Evaluation of Seven Function-Known Candidate Genes for their Effects on Improving Drought Resistance of Transgenic Rice under Field Conditions[J]. Molecular Plant 2009,2(1):73-83
[53]Bing Yue, Weiya Xue, Lizhong Xiong,et.al. Genetic Basis of Drought Resistance at Reproductive Stage in Rice:Separation of Drought Tolerance From Drought Avoidance[J]. Genetics,2006,172:1213-1228
[54]Benze Xiao, Yuemin Huang, Ning Tang,et.al. Over-expression of a LEA gene in rice improves drought resistance under the field conditions[J]. Theoretical and Applie Genetics,2007,115:36-46
[55]Bingliang Wan, Yongjun Lin, Tongmin Mou. Expression of rice Ca2+-dependent protein kinases (CDPKs) genes under different environmental stresses[J]. FEBS Letters.2007,581:1179-1189
[56]张妍,王瑛,梁玉玲,等.转LEA3基因水稻的抗性分析[J].河北农业大学学报,2005,28(5)：33-36
[57]Wen-Xue Lia, Youko Oono, Jianhua Zhuc,et.al. The Arabidopsis NFYA5 transcrip-tion Factor Is Regulated Transcriptionally and Posttranscriptionally to Promote Drought resistance[J]. The Plant Cell, 2008,20:2238-2251
[58]Xiang-Huan Cui, Fu-Shun Hao, Hui Chen,et.al.Expression of the Vicia faba VfPIPl gene in Arabidopsis thaliana plants improves their drought resistance [J].Journal of Plant Research,2008,121: 207-214
[59]Mie Kasuga, Qiang Liul, Setsuko Miural,et.al.Improving plant drought, salt, andfreezing tolerance by gene transfer of a single stress-inducible transcription factor[J].Nature biotechnology,1999,17(3): 287-291
[60]Eleonora Cominelli, Massimo Galbiati, Alain Vavasseur,et.al.A Guard-Cell-Specific MYB Transcription Factor Regulates Stomatal Movements and Plant Drought Tolerance[J]. Current Biology, 2005,15:1196-1200
[61]郝岗平,吴忠义,陈茂盛,等.ATHK1基因调节拟南芥渗透胁迫信号转导过程[J].植物生理与分子生物学学报,2004,30(5)：553-560
[62]郝岗平,吴忠义,陈茂盛,等.拟南芥CBF4基因位点的单核苷酸多态性(SNP)变化与抗旱表型的相应性[J].农业生物技术学报,2004,12(2)：122-131
[63]戚元成,张小强,刘卫群,等；过量表达谷胱甘肽转移酶基因对转基因拟南芥抗旱能力的影响[J].植物生理学通讯,2008,44(2)：268-270
[64]张微微,车代弟,张兴,等.CBF1转录因子基因的克隆及两种启动子调控下的植物表达载体的构建[J].分子植物育种,2005,3(4)：493-497
[65]姜丽丽,邸宏,林忠平,等.蓝铜蛋白类似基因BcBCP1转化马铃薯及其抗旱性的改良[J].中国蔬菜,2009(2)：7-11
[66]黄福平,陈荣冰.茶树抗旱指标研究指标[J].福建茶叶,2000,3：25
[67]许允文等,Water requirement characteristic of tea plant and its effect on tea yield and quality[M].proceedings of internationalTea-Quality-Human HealthSymposium.1987.Hang zhou
[68]潘根生.茶园喷灌的小气候效应及其对茶叶产量品质的影响[J].浙江农业大学学报,1981,(1)：49-61
[69]李金昌.早热季节不同喷水量对秋茶产量和品质的影响[J].中国茶叶,1987(5)：24
[70]罗凡,王云,李春华,等.四川茶树品种研究现状与发展趋势[J].贵州科学.2008,26(2)：52-57
[71]杨维时,程徽儿,胡绍德.利用合肥种茶逆境选育“多抗香”茶树品种[J].蚕桑茶叶通讯,2000,4：33-34
[72]杨亚军.中国茶树栽培学[M].上海科学技术出版社,2005.21-31
[73]曾勤,王立.基因工程在茶树育种领域的应用前景[J].中国茶叶,1994,2：20-21
[74]束际林.茶树抗寒性叶结构鉴定和筛选[J].中国茶叶,1992(3)：19-21
[75]B C Barbora,D.N.Barua, B.Brea. Tea Breeding at Tocklai[J]. Two and a Bud,1996,43(1):3-9
[76]潘根生,吴伯千,沈生荣,等.水分胁迫过程中茶树新梢内源激素水平的消长及其与耐旱性的关系[J].中国农业科学,1996,29(5)：9-15
[77]陆德彪,童启庆,骆耀平,等.茶树种质资源抗旱性的综合评价[J].浙江农业大学学报,1995,21(5)：447-450
[78]Rajan K Mishra,Swati SEN-Mandi.Genome analysis and isozyme studies for developing molecular markers associated with drought tolerance in tea plant[A]. Production Proceedings of 2001 ICOS (Session Ⅱ) [C] Shizuoka,Japan,2001:66-69
[79]刘玉英.茶树抗旱生理生化机制的研究[D].西南大学,2006,5,15
[80]魏鹏.茶树抗旱性部分生理生化指标的研究[D].西南农业大学,2003,5,1
[81]郭春芳,孙云,赖呈纯,等.聚乙二醇胁迫下茶树叶片的蛋白质组分析[J].茶叶科学,2009,29(2)：79-88
[82]梅菊芬,王新超,杨亚军,等.茶树冷驯化过程中基因表达差异的初步分析[J].茶叶科学,2007,27(4)：286-292
[83]陈暄,房婉萍,雏中伟,等.茶树冷胁迫诱导抗寒基因CBF的克隆与表达分析[J].茶叶科学,2009,29(1)：53-59
[84]邹中伟,房婉萍,张定,等.低温胁迫下茶树基因表达的差异分析[J].茶叶科学2008,28(4)：249-254
[85]项鹏,李树浓.基因表达差异显示方法研究进展[J].国外医学临床生物化学与检验学分册,2001,22(4)：971-973
[86]叶纨芝,曹家树,曾广文.：mRNA差别显示技术及其应用[J].生物技术,1999,9(4)：26-30
[87]P.Liang.A.D pardee. Differential display of eukaryotic messenger RNA by means of the poly merase chain reaction[J].Science,1992,257(5027):967-970
[88]支立峰,余涛,彭茂民,等.利用改进的DDRT-PCR分离并鉴定水稻细胞缺铁胁迫相关cDNA克隆[J].武汉植物学研究,2005,23(1)：7-10
[89]陆海,张建秋,张玉玲,等.一种优化的mRNA差异显示技术分析白刺盐碱胁迫表达相关基因研究[J].成都大学学报(自然科学版),2004,23(2)：1-6
[90]柳俊,谢从华.马铃薯块茎发育机理及其基因表达[J].植物学通报,2001,18(5)：531-539
[91]林金科,郑金贵.外源诱导提高茶树新梢EGCG含量过程的相关基因分离[J].茶叶科学,2004,24(4)：260-265
[92]王新超,赵丽萍,姚明哲,等.安吉白茶正常与白化叶片基因表达差异的初步研究[J].茶叶科学,2008,28(1)：50-55
[93]韦朝领,高香凤,叶爱华,等.基于DDRT-PCR研究茶树对茶尺蠖取食诱导的基因表达谱差异[J].茶叶科学,2007,27(2)：133-140
[94]杨鑫光.霸王对干旱胁迫的响应及根系提水研究[D].兰州大学,2006,6,1
[95]沙莉娜,付凤玲,李晚忱.玉米耐旱自交系在干旱条件下的mRNA差异表达[J].核农学报,2006,20(5)：365-370
[96]Ashok K. Jain, Sheikh Mehboob Basha, C. Corley Holbrook. Identification of drought responsive transcripts in peanut(Arachis hypogaea L.) [J]. Electronic Journal of Biotechnology,2001,4(2):59-67
[97]张立早,吴平,金明,等.利用DD-PCR技术分析水稻铝诱导基因的表达差异[J].中国农业科学1997,30(5)：71-74
[98]郭宾会,高双成,景蕊莲,等.小麦苗期水分胁迫诱导差异表达cDNA的研究[J].生物技术通报,2003，2：40-45
[99]李大力.一种从富含次生物质的植物中提取RNA的方法[J].南京理工大学学报,2001,25(5)：547-549
[100]李娟,刘硕谦,刘仲华,等.一种改良的茶树高质量RNA快速提取方法[J].福建茶叶,2007,4：34-35
[101]江昌俊,王朝霞,李叶云.茶叶中提取总RNA的研究[J].茶叶科学,2009,20(1)：27-29
[102]史成颖,宛晓春,江昌俊,等.提取高质量茶树总RNA的方法研究[J].安徽农业大学学报,2007,34(3)：360-363
[103]鲁黎明,王林忠,杨铁钊.烟草总RNA高效提取方法研究[J].分子植物育种,2006,4(6)：157-160
[104]王昆,王颖,鲍永利,等.提取高质量人参RNA方法研究[J].生物化学与生物物理进展,2006,33(1)：95-99
[105]杨新艳,刘培欣,单文鲁,等.mRNA差异显示技术及其改进[J].动物医学进展,2004,25(6)：19-21
[106]Zhao S.,O oi S L.,Pardee A.B.New primer strategy improves precision of differential display[J]. Biotechnique,1995,18(5):842-846,848,850
[107]Bauer D.,Muller H.,Reich J,et.al.Identification of differentially expressed mRNA species by an improved diplay technique(DDRT-PCR), [J].Nucleic Acids Researche,1993,21(18):4272-4280
[108]梁德勇,王晓民,崔振中,等.银染mRNA差异显示方法的条件优化[J].中国神经科学杂志,1999,15(2)：151-155
[109]闫红飞.小麦叶锈病与Lr38互作诱导抗病基因表达DDRT-PCR分析[D].河北农业大学,2003,6,1
[110]陈立松,刘星辉.水分胁迫对荔枝叶片氮和核酸代谢的影响及其与抗旱性的关系[J].植物生理学报,1999,25(1)：49-56
[111]刘娟,冯群芳,张杰.二氢黄酮醇4-还原酶基因(DFR)与花色的修饰[J].植物生理学通讯,2005,41(6)：715-719
[112]ANNAMARYJU D S.Antioxidant ability of anthocyanins against ascorbic acid oxidation[J]. Phytochemistroy,1997,45(4):671-674.
[113]赵云龙,王世雷.植物花青素研究进展[J].安徽农业科学,2008,36(8)：3095-3097
[114]许志茹,李春雷,崔国新,等.植物花青素合成中的MYB蛋白[J].植物生理学通讯,2008,44(3)：597-604
[115]刘蕾,杜海,唐晓凤,等.MYB转录因子在植物抗逆胁迫中的作用及其分子机理[J].遗传,2008,30(10)：1265-1271
[116]Hoeren FU, Dolferus R, Wu Y, Peacock WJ, Dennis ES. Evidence for a role for AtMYB2 in the induction of theArabidopsis alcohol dehydrogenase gene (ADH1) by low oxygen[J].Genetics,1998,149 (2):479-490.
[117]Cominelli E, Galbiati M, Vavasseur A,et.al. Aguard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol,2005,15(13):1196-2000.
[118]Vannini C, Locatelli F, Bracale M,et.al. Overexpression of the rice Osmyb4 gene increases chilling andfreezing tolerance of Arabidopsis thaliana plants[J].Plant Journal,2004,37(1):115-127.
[119]许丽颖,赫玉苹,王刚,等.水分胁迫对紫色李叶片色素含量与PAL活性的影响[J].吉林农业大学学报,2007,29(2)：168-172
[120]Kashmir Singh,Arti Rani,Asosii Paul, et.al. Differential display mediated cloning of anthocyanidin reductase gene from tea(camellia sinensis) and its relationship with the concerntration of epicatechics[J]. oxford Journals tree physiology 2009,26(6):837-846
[2]石凤翎,郭晓霞,李红.扁蓿豆抗旱形态解剖结构观察与分析[J].干旱地区农业研究,2005,23(2)：115-118
[3]路贵和,黄淮海地区不同抗旱类型大豆种质资源根系特征及抗旱性研究[J].山西农业科学,2000,28(2)：37.-40
[4]曹雄,张小虎,任小俊.灰色理论在绿豆抗旱育种中的应用[J].国外农学-杂粮作物,1999,9(3)：19-21
[5]Carlos German Munoz-Perea, Henry Terdn, Richard G. Allen, James L. Wright,et.al. Selection for Drought Resistance in Dry Bean Landraces and Cultivars[J].Crop Science,2006,46:2111-2120
[6]Shree P. Singh. Drought Resistance in the Race Durango Dry Bean Landraces and Cultivars[J]. Agronomy Journal,2007,99:1219-1225
[7]毛淑蕊,李墨霞,万勇善,等,渗透胁迫对花生不定根再生及生长特性的影响[J].山东农业大学学报(自然科学版),2009,40(3)：329-33
[8]王育红,姚宇卿,吕军杰,等.花生抗旱指标研究初报[J].干旱地区农业研究,2002,20(3)：89-92
[9]P. Songsri, S. Jogloy, T. Kesmala,et.al. Heritability of Drought Resistance Traits and Correlation of Drought Resistance and Agronomic Traits in Peanut[J].Crop Science,2008,48:2245-2253
[10]Kpoghomou B K. Screening for drought tolerance soybean germination and its relationship to seedling response[J] Journal Agronomy & Crop Science,1990,164:153-159
[11]安永平,强爱玲,张媛媛,等.渗透胁迫下水稻种子萌发特性及抗旱性鉴定指标研究[J].植物遗传资源学报,2006,7(4)：421-426
[12]Jerome Bernier,Gary N Atlin,Rachid Serraj,et.al. Review:Breeding upland rice for drought resistance[J]. Journal of the Science of Food and Agriculture.2008,88:927-939
[13]Bing Yue, Lizhong Xiong, Weiya Xue,et.al. Genetic analysis for drought resistance of rice at re-productive stage in field with different types of soil[J]. Theoretical and Applied Genetics,2005,111 (6): 1127-1136
[14]陈凤梅,程建峰,潘晓云,等.杂交稻抗旱性状的筛选研究[J].杂交水稻2001,16(4)：51-54
[15]Z. Peleg, T. Fahima, S. Abbo,et.al.Genetic diversity for drought resistance in wild emmer wheat wheat and its ecogeographical associations[J]. Plant, Cell and Environment.2005,28:176-191
[16]R.M. Trethowan, M. Reymolds. Drought ResistanceE:Genetic Approaches for Improveing Productivity Under Stress[J]. Wheat Production in Stressed Environmen-ts.2007:289-299
[17]刘桂茹,葛淑俊,王静华,等.PEG对小麦花药出愈率的影响[J].河北农业大学学报,2001,24(4)：25-27
[18]M.J. Foulkes, R. Sylvester-Bradley, R. Weightman,et.al. Identifying physiological traits associat-ed with improved drought resistance in winter wheat[J].Field Crops Research,2007,103:11-24
[19]王瑾,刘桂茹,杨学举.胁迫下小麦再生植株根系特性与抗旱性的关系[J].麦类作物学报,2006,26(3)：117-119
[20]张振平,孙世贤,张悦,等.玉米叶部形态指标与抗旱性的关系研究[J].玉米科学,2009,17(3)：68-70
[21]树宾,王有德,等.西北旱农区玉米抗旱性的相关研究[J].新疆农业科,2001,38(2)：58-59
[22]赵美玲.玉米各生育时期抗旱性鉴定指标的研究[J].中国农学通报2009,25(12)：66-68
[23]张雪妍,刘传亮,王俊娟.PEG胁迫方法评价棉花幼苗耐旱性研究[J].棉花学报,2007,19(3)：205-209
[24]Herv6 Cochard, S.Tete Barigah, Marc Kleinhentz,et.al. Is xylem cavitation resistance a relevant criterion for screening drought resistance among Prunus species?[J]. Journal of Plant Physiology,2008, 165:976-982
[25]胡向敏,云锦凤.针茅族3种多年生牧草苗期抗旱性的比较研究[J].牧草与饲料科学,2009,3(2)：18-21
[26]赵相勇,陈培燕,袁庆华,等.高羊茅苗期抗旱性的生理研究[J].西南农业报,2009,22(3)：621-624
[27]焦树英,李永强,沙依拉.沙尔合提.等.干旱胁迫对3种狼尾草种子萌发和幼苗生长的影响[J].西北植物学报,2009,29(2)：0308-0313
[28]李晚忱,付凤玲,袁佐清.玉米苗期耐旱性鉴定方法研究[J].西南农业学报,2001,14(3)：29-32
[29]孙祖东,陈怀珠,杨守臻.苗期干早胁迫对大豆生长发育的影响与耐早材料的筛选[J].厂西农业科学,2002,1：11-13
[30]董立峰,尹秀玲,等.小麦离体叶片室内自然干化失水率与品种抗旱性关系的研究[J].河北农业技术师范学院学报,1997,11(1)：50-54
[31]张春霞,谢惠民,王婧,等.小麦品种叶片水势与抗旱节水性的关系[J].麦类作物报,2009,29(3)：453-459
[32]杨琳,景继海,赵佰图.旱地小麦品种和自育高代品系资源抗旱性鉴定研[J].陕西农业科 学,2009,4：42-46
[33]郭数进,李贵全.大豆生理指标与抗旱性关系的研究[J].河南农业科学,2009,6：38-41
[34]郭数进,李贵全.干旱胁迫对不同大豆品系酶类变化的影响[J].大豆科学,2009,28(3)：445-449
[35]郭数进,李贵全.干旱胁迫下不同大豆品系生理指标变化与抗旱性关系的研究[J].中国种业,2009，6：38-40
[36]赵玲,马向丽,邓祥升,等.干旱胁迫对草地早熟禾抗旱生理的影响[J].草业与畜牧,2009,6：37-41
[37]刘云开,方宝华,庞冰,等.水稻品种节水抗旱适应性筛选研究[J].湖南农业科学,2009,(5)：24-26
[38]罗俊,林彦铨,张木清.甘蔗活性氧代谢对水分胁迫的响应[J].福建农业大学学报,2009,29(4)：405-410
[39]罗俊,林彦铨,吕建林,等.水分胁迫对甘蔗叶片光合性能的影响[J].中国农业科学,2000,33(4)：100-102
[40]薛立,薛晔,任向荣,等.PEG模拟干旱条件下尾叶桉和枫香苗木的生理响应[J].生态环境学报,2009,18(2)：614-620
[41]陈由强,朱锦懋,叶冰莹.水分胁迫对芒果(Mangifera indica L.)幼叶细胞活性氧伤害的影响[J].生命科学研究,2000,4(1)：60-64
[42]叶冰莹,陈由强,朱锦懋,等.水分胁迫对三种木麻黄小枝活性氧伤害的研究[J].福建师范大学学报(自然科学版),2000,16(1)：76-79
[43]陈静,邢菊香,李春红.绣球绣线菊和土庄绣线菊强的抗旱生理特性比较[J].内蒙古农业大学学报,2009,30(1)：295-298
[44]李磊,贾志清,宁虎森,等.水分胁迫下2种沙枣的抗旱性比较[J].林业科学研究,2009,22(3)：335-342
[45]荣红颖,张立全,杨凤萍,等.DREBIB基因在转基因小麦后代的稳定表达[J].分子植物育种,2009,7(3)：437-443
[46]王婧,谢惠民,张春霞,等.冬小麦幼苗期水分胁迫应答蛋白遗传分析及基因初步定位[J].麦类作物学报2009,29(3)：396-400
[47]杜丽璞,徐惠君,叶兴国,等.小麦转TPS基因植株的获得及其初步功能鉴定[J].麦类作物学报2007,27(3)：369-373
[48]徐重益.小麦抗旱相关基因TaPP2Ac/a的分离、定位与功能验证[D].中国农业科学院作物科学研究所,2007,7
[49]王静英,李永春,王潇,等.小麦TaLEA4基因的克隆和表达[J].麦类作物学报,2008,28(2)：183- 186
[50]Hong hong Hu,Mingqiu Dai,Jialing Yao.et.al. Overexpressing a NAM, ATAF, and CUC(NAC) Transcription factor enhances drought resistance and salt tolerance in rice[J]. PANS,2006,103(35): 1297-1292
[51]Yuemin Huang,Benze Xiao,Lizhong Xiong. Characterization of a stress responsive proteinase inhibitor gene with positive effect in improving drought resistance in rice[J]. Planta,2007,226:73-85
[52]Ben-Ze Xiao, Xi Chen, Cheng-Bin Xiang,et.al. Evaluation of Seven Function-Known Candidate Genes for their Effects on Improving Drought Resistance of Transgenic Rice under Field Conditions[J]. Molecular Plant 2009,2(1):73-83
[53]Bing Yue, Weiya Xue, Lizhong Xiong,et.al. Genetic Basis of Drought Resistance at Reproductive Stage in Rice:Separation of Drought Tolerance From Drought Avoidance[J]. Genetics,2006,172:1213-1228
[54]Benze Xiao, Yuemin Huang, Ning Tang,et.al. Over-expression of a LEA gene in rice improves drought resistance under the field conditions[J]. Theoretical and Applie Genetics,2007,115:36-46
[55]Bingliang Wan, Yongjun Lin, Tongmin Mou. Expression of rice Ca2+-dependent protein kinases (CDPKs) genes under different environmental stresses[J]. FEBS Letters.2007,581:1179-1189
[56]张妍,王瑛,梁玉玲,等.转LEA3基因水稻的抗性分析[J].河北农业大学学报,2005,28(5)：33-36
[57]Wen-Xue Lia, Youko Oono, Jianhua Zhuc,et.al. The Arabidopsis NFYA5 transcrip-tion Factor Is Regulated Transcriptionally and Posttranscriptionally to Promote Drought resistance[J]. The Plant Cell, 2008,20:2238-2251
[58]Xiang-Huan Cui, Fu-Shun Hao, Hui Chen,et.al.Expression of the Vicia faba VfPIPl gene in Arabidopsis thaliana plants improves their drought resistance [J].Journal of Plant Research,2008,121: 207-214
[59]Mie Kasuga, Qiang Liul, Setsuko Miural,et.al.Improving plant drought, salt, andfreezing tolerance by gene transfer of a single stress-inducible transcription factor[J].Nature biotechnology,1999,17(3): 287-291
[60]Eleonora Cominelli, Massimo Galbiati, Alain Vavasseur,et.al.A Guard-Cell-Specific MYB Transcription Factor Regulates Stomatal Movements and Plant Drought Tolerance[J]. Current Biology, 2005,15:1196-1200
[61]郝岗平,吴忠义,陈茂盛,等.ATHK1基因调节拟南芥渗透胁迫信号转导过程[J].植物生理与分子生物学学报,2004,30(5)：553-560
[62]郝岗平,吴忠义,陈茂盛,等.拟南芥CBF4基因位点的单核苷酸多态性(SNP)变化与抗旱表型的相应性[J].农业生物技术学报,2004,12(2)：122-131
[63]戚元成,张小强,刘卫群,等；过量表达谷胱甘肽转移酶基因对转基因拟南芥抗旱能力的影响[J].植物生理学通讯,2008,44(2)：268-270
[64]张微微,车代弟,张兴,等.CBF1转录因子基因的克隆及两种启动子调控下的植物表达载体的构建[J].分子植物育种,2005,3(4)：493-497
[65]姜丽丽,邸宏,林忠平,等.蓝铜蛋白类似基因BcBCP1转化马铃薯及其抗旱性的改良[J].中国蔬菜,2009(2)：7-11
[66]黄福平,陈荣冰.茶树抗旱指标研究指标[J].福建茶叶,2000,3：25
[67]许允文等,Water requirement characteristic of tea plant and its effect on tea yield and quality[M].proceedings of internationalTea-Quality-Human HealthSymposium.1987.Hang zhou
[68]潘根生.茶园喷灌的小气候效应及其对茶叶产量品质的影响[J].浙江农业大学学报,1981,(1)：49-61
[69]李金昌.早热季节不同喷水量对秋茶产量和品质的影响[J].中国茶叶,1987(5)：24
[70]罗凡,王云,李春华,等.四川茶树品种研究现状与发展趋势[J].贵州科学.2008,26(2)：52-57
[71]杨维时,程徽儿,胡绍德.利用合肥种茶逆境选育“多抗香”茶树品种[J].蚕桑茶叶通讯,2000,4：33-34
[72]杨亚军.中国茶树栽培学[M].上海科学技术出版社,2005.21-31
[73]曾勤,王立.基因工程在茶树育种领域的应用前景[J].中国茶叶,1994,2：20-21
[74]束际林.茶树抗寒性叶结构鉴定和筛选[J].中国茶叶,1992(3)：19-21
[75]B C Barbora,D.N.Barua, B.Brea. Tea Breeding at Tocklai[J]. Two and a Bud,1996,43(1):3-9
[76]潘根生,吴伯千,沈生荣,等.水分胁迫过程中茶树新梢内源激素水平的消长及其与耐旱性的关系[J].中国农业科学,1996,29(5)：9-15
[77]陆德彪,童启庆,骆耀平,等.茶树种质资源抗旱性的综合评价[J].浙江农业大学学报,1995,21(5)：447-450
[78]Rajan K Mishra,Swati SEN-Mandi.Genome analysis and isozyme studies for developing molecular markers associated with drought tolerance in tea plant[A]. Production Proceedings of 2001 ICOS (Session Ⅱ) [C] Shizuoka,Japan,2001:66-69
[79]刘玉英.茶树抗旱生理生化机制的研究[D].西南大学,2006,5,15
[80]魏鹏.茶树抗旱性部分生理生化指标的研究[D].西南农业大学,2003,5,1
[81]郭春芳,孙云,赖呈纯,等.聚乙二醇胁迫下茶树叶片的蛋白质组分析[J].茶叶科学,2009,29(2)：79-88
[82]梅菊芬,王新超,杨亚军,等.茶树冷驯化过程中基因表达差异的初步分析[J].茶叶科学,2007,27(4)：286-292
[83]陈暄,房婉萍,雏中伟,等.茶树冷胁迫诱导抗寒基因CBF的克隆与表达分析[J].茶叶科学,2009,29(1)：53-59
[84]邹中伟,房婉萍,张定,等.低温胁迫下茶树基因表达的差异分析[J].茶叶科学2008,28(4)：249-254
[85]项鹏,李树浓.基因表达差异显示方法研究进展[J].国外医学临床生物化学与检验学分册,2001,22(4)：971-973
[86]叶纨芝,曹家树,曾广文.：mRNA差别显示技术及其应用[J].生物技术,1999,9(4)：26-30
[87]P.Liang.A.D pardee. Differential display of eukaryotic messenger RNA by means of the poly merase chain reaction[J].Science,1992,257(5027):967-970
[88]支立峰,余涛,彭茂民,等.利用改进的DDRT-PCR分离并鉴定水稻细胞缺铁胁迫相关cDNA克隆[J].武汉植物学研究,2005,23(1)：7-10
[89]陆海,张建秋,张玉玲,等.一种优化的mRNA差异显示技术分析白刺盐碱胁迫表达相关基因研究[J].成都大学学报(自然科学版),2004,23(2)：1-6
[90]柳俊,谢从华.马铃薯块茎发育机理及其基因表达[J].植物学通报,2001,18(5)：531-539
[91]林金科,郑金贵.外源诱导提高茶树新梢EGCG含量过程的相关基因分离[J].茶叶科学,2004,24(4)：260-265
[92]王新超,赵丽萍,姚明哲,等.安吉白茶正常与白化叶片基因表达差异的初步研究[J].茶叶科学,2008,28(1)：50-55
[93]韦朝领,高香凤,叶爱华,等.基于DDRT-PCR研究茶树对茶尺蠖取食诱导的基因表达谱差异[J].茶叶科学,2007,27(2)：133-140
[94]杨鑫光.霸王对干旱胁迫的响应及根系提水研究[D].兰州大学,2006,6,1
[95]沙莉娜,付凤玲,李晚忱.玉米耐旱自交系在干旱条件下的mRNA差异表达[J].核农学报,2006,20(5)：365-370
[96]Ashok K. Jain, Sheikh Mehboob Basha, C. Corley Holbrook. Identification of drought responsive transcripts in peanut(Arachis hypogaea L.) [J]. Electronic Journal of Biotechnology,2001,4(2):59-67
[97]张立早,吴平,金明,等.利用DD-PCR技术分析水稻铝诱导基因的表达差异[J].中国农业科学1997,30(5)：71-74
[98]郭宾会,高双成,景蕊莲,等.小麦苗期水分胁迫诱导差异表达cDNA的研究[J].生物技术通报,2003，2：40-45
[99]李大力.一种从富含次生物质的植物中提取RNA的方法[J].南京理工大学学报,2001,25(5)：547-549
[100]李娟,刘硕谦,刘仲华,等.一种改良的茶树高质量RNA快速提取方法[J].福建茶叶,2007,4：34-35
[101]江昌俊,王朝霞,李叶云.茶叶中提取总RNA的研究[J].茶叶科学,2009,20(1)：27-29
[102]史成颖,宛晓春,江昌俊,等.提取高质量茶树总RNA的方法研究[J].安徽农业大学学报,2007,34(3)：360-363
[103]鲁黎明,王林忠,杨铁钊.烟草总RNA高效提取方法研究[J].分子植物育种,2006,4(6)：157-160
[104]王昆,王颖,鲍永利,等.提取高质量人参RNA方法研究[J].生物化学与生物物理进展,2006,33(1)：95-99
[105]杨新艳,刘培欣,单文鲁,等.mRNA差异显示技术及其改进[J].动物医学进展,2004,25(6)：19-21
[106]Zhao S.,O oi S L.,Pardee A.B.New primer strategy improves precision of differential display[J]. Biotechnique,1995,18(5):842-846,848,850
[107]Bauer D.,Muller H.,Reich J,et.al.Identification of differentially expressed mRNA species by an improved diplay technique(DDRT-PCR), [J].Nucleic Acids Researche,1993,21(18):4272-4280
[108]梁德勇,王晓民,崔振中,等.银染mRNA差异显示方法的条件优化[J].中国神经科学杂志,1999,15(2)：151-155
[109]闫红飞.小麦叶锈病与Lr38互作诱导抗病基因表达DDRT-PCR分析[D].河北农业大学,2003,6,1
[110]陈立松,刘星辉.水分胁迫对荔枝叶片氮和核酸代谢的影响及其与抗旱性的关系[J].植物生理学报,1999,25(1)：49-56
[111]刘娟,冯群芳,张杰.二氢黄酮醇4-还原酶基因(DFR)与花色的修饰[J].植物生理学通讯,2005,41(6)：715-719
[112]ANNAMARYJU D S.Antioxidant ability of anthocyanins against ascorbic acid oxidation[J]. Phytochemistroy,1997,45(4):671-674.
[113]赵云龙,王世雷.植物花青素研究进展[J].安徽农业科学,2008,36(8)：3095-3097
[114]许志茹,李春雷,崔国新,等.植物花青素合成中的MYB蛋白[J].植物生理学通讯,2008,44(3)：597-604
[115]刘蕾,杜海,唐晓凤,等.MYB转录因子在植物抗逆胁迫中的作用及其分子机理[J].遗传,2008,30(10)：1265-1271
[116]Hoeren FU, Dolferus R, Wu Y, Peacock WJ, Dennis ES. Evidence for a role for AtMYB2 in the induction of theArabidopsis alcohol dehydrogenase gene (ADH1) by low oxygen[J].Genetics,1998,149 (2):479-490.
[117]Cominelli E, Galbiati M, Vavasseur A,et.al. Aguard-cell-specific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol,2005,15(13):1196-2000.
[118]Vannini C, Locatelli F, Bracale M,et.al. Overexpression of the rice Osmyb4 gene increases chilling andfreezing tolerance of Arabidopsis thaliana plants[J].Plant Journal,2004,37(1):115-127.
[119]许丽颖,赫玉苹,王刚,等.水分胁迫对紫色李叶片色素含量与PAL活性的影响[J].吉林农业大学学报,2007,29(2)：168-172
[120]Kashmir Singh,Arti Rani,Asosii Paul, et.al. Differential display mediated cloning of anthocyanidin reductase gene from tea(camellia sinensis) and its relationship with the concerntration of epicatechics[J]. oxford Journals tree physiology 2009,26(6):837-846