光质对三种芽苗菜生理特性及品质的影响
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摘要
本试验采用新型半导体光源发光二极管(LED)精量调制光质(红光,640±5 nm;蓝光,460±5 nm;红蓝组合光,红光:蓝光=3:1,灯的数量比),以普通日光灯(白光)为对照,就不同光质对三种芽苗菜(萝卜、豌豆、香椿)生理特性和品质的影响进行了研究。研究结果表明:
     1.不同光质处理对芽苗菜生长有显著影响。红光处理下芽苗菜植株最高、鲜重最大,说明照射红光芽苗菜营养生长旺盛,能提高芽苗菜的产量。红蓝混合光处理与对照处理对芽苗菜生长的影响差异不明显。蓝光处理下芽苗菜植株最矮、鲜重最小,说明照射蓝光抑制了芽苗菜的生长,使芽苗菜的产量下降。
     2.不同光质处理对芽苗菜生理特性有较大影响。随着芽苗菜的生长,叶绿素含量总体呈先增加后减少的趋势。各LED有色光质处理下芽苗菜叶绿素含量都明显高于对照,说明红光和蓝光都有利于叶绿素的合成。萝卜苗与豌豆苗在生长期可溶性糖含量逐渐下降,而香椿苗可溶性糖含量先上升后下降。在芽苗菜整个生长过程中,可溶性蛋白含量先下降后上升。红光处理下芽苗菜可溶性糖含量明显高于对照和其他处理,但可溶性蛋白含量却明显低于对照和其他处理。蓝光处理对芽苗菜可溶性糖、可溶性蛋白含量的影响与红光处理正好相反。红蓝混合光处理对芽苗菜可溶性糖、可溶性蛋白含量总体高于对照处理。总体上芽苗菜可溶性糖含量大小顺序为红光>红蓝混合光>对照>蓝光,芽苗菜可溶性蛋白含量大小顺序为蓝光>红蓝混合光>对照>红光。这说明红光促进芽苗菜可溶性糖含量的增加,而不利于可溶性蛋白的积累;蓝光处理促进芽苗菜可溶性蛋白含量的增加,而不利于可溶性糖的积累。
     3.不同光质处理对芽苗菜商品、营养品质影响不同。豌豆苗与香椿苗维生素C(Vc)含量先上升后下降,而萝卜苗Vc含量逐渐下降。红光和蓝光处理下Vc含量低于红蓝混合光处理下,这说明与混合光相比,单色光不利于Vc的积累。随着芽苗菜的生长,粗纤维素含量逐渐增加,由于粗纤维素影响口感,应该适时采收。粗纤维素含量以红光下最高,红蓝混合光次之,蓝光下最低。在芽苗菜整个生长过程中,游离氨基酸含量先上升后下降。从总体趋势来看,蓝光处理下的芽苗菜游离氨基酸含量明显高于其他光质处理,而红光处理下最低。豌豆苗与香椿苗在生长期类黄酮含量先上升后下降,而萝卜苗类黄酮含量逐渐下降。对照与各LED有色光质处理相比明显提高了芽苗菜类黄酮含量,说明白光中有其他光质促进了芽苗菜类黄酮的合成,芽苗菜类黄酮含量大小顺序为对照>蓝光>红蓝混合光>红光。随着芽苗菜的生长,硝酸盐含量逐渐提高,硝酸盐是对人体有害物质,应该适时采收芽苗菜。蓝光与红蓝混合光处理与对照相比都能提高芽苗菜硝酸盐含量,而红光处理降低了芽苗菜硝酸盐含量。
Using the red (590-670 nm; peak at 640 nm), blue (420-510 nm; peak at 460 nm), red-blue (red: blue =3:1) light-emitting diode and white fluorescent as light sources, the effects of different light quality on the physiological characteristic and quality in three kinds of sprouts vegetables(radish, pea, toona sinensis) were studied. The main results were as following:
     1.Light quality had significant effects on growth of sprouts vegetables. The plant height and fresh weight were the highest under red light.It was concluded that the red light can enhanced the growth and yield of the sprouts vegetables. The effects on the sprouts vegetables under red-blue light as much as under white light.. The plant height and fresh weight were the lowest under blue light. It was concluded that the blue light can inhabited the growth and yield of the sprouts vegetables.
     2.Light quality had great effects on Physiological characteristics of sprouts vegetables.As the sprouts vegetables were growing, the chlorophyll content always firstly increased and then fell. The contents of the chlorophyll under red light, blue light and red-blue light were higher than that of white light treatment.. It showed that red light and blue light were all benefit to the biosynthesis of the chlorophyll. The soluble sugar content of radish and pea sprouts gradually fell , and the toona sinensis’firstly increased and then fell in their growth time. The soluble protein content of sprouts vegetables firstly fell and then increased in their growth time. As a whole, the sequence of soluble sugar content in sprouts vegetables ranged that: RL>RBL>WL>BL,and and that of s soluble protein content was BL>RBL>WL>RL. It was concluded that the red light increase the content of soluble sugar,but t the red light was not propitious to the accumulation of soluble protein. It suggested that the blue light increase the content of soluble protein,but t the red light was not propitious to the accumulation of sugar soluble sugar.
     3.There were more differences of sprouts vegetables commodity and nutrition quality under different light quality. As the pea and toona sinensis sprouts were growing, the vitamin C content always firstly increased and then fell. The vitamin C content of radish gradually fell in in their growth time. The contents of vitamin C under red light and blue light were lower than those of red-blue light. It showed that the single light was not propitious to the accumulation of vitamin C compared with mixture light..As the sprouts vegetables were growing, The crude fibre content gradually increased,and the crude fibre affectd the taste,we should pat harvest. The content of crude fibre acid was highest under red light and lowest under blue light..Free amino acid content of sprouts vegetables under different light quality firstly increased and then fell in their growth time. As a whole, The free amino acid content of sprouts vegetables under blue light were significant higher than those under the other light. Those under red light were lowest.The flavonoids content of pea and toona sinensis sprouts firstly increased and then fell ,and the radish’gradually fell in their growth time. The content of flavonoids was highest under the white light treatment, It suggested that there were possiblely another light quality could flavonoids biosynthesis was accelerated in white light.The sequence of flavonoids content in sprouts vegetables ranged that: WL>BL>RBL>RL. .As the sprouts vegetables were growing, The nitrate content gradually increased,and the crude fibre affectd the health,we should pat harvest. The nitrate content of sprouts vegetables under blue and red-blue light were higher than those under white light.But the nitrate biosynthesis was inhabited under red light,
引文
安华明,陈力耕,樊卫国,等.刺梨果实中维生素C积累与相关酶活性的关系[J].植物生理与分子生物学学报,2005,31(4):431~436.
    曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007,34~95.
    陈强,刘世琦,张自坤,等.不同LED光源对番茄果实转色期品质的影响[J].农业工程学报,2009,25 (5):156~161.
    陈大清.不同光质和激动素对拟南芥(ArabidoPsisthallanaL)幼苗光形态建成影响的研究[D].华南师范大学博士论文,2002,14.
    储钟稀,童哲.不同光质对黄瓜叶片光合特性的影响.植物学报,1999,41(8):867~870.
    戴绍军,王洋,阎秀峰,等.滤光膜对喜树幼苗叶片生长和喜树碱含量的影响[J].生态学报,2004,24(5):869~875.
    邓江明,宾金华,潘瑞炽.光质对水稻幼苗初级氮同化的影响[J].植物学报,2000,42(3):234~238.
    杜健芳,叶步青.光质对油菜幼苗生长及抗氧化酶活性的影响.植物学通报2002,19(6).743~745.
    郭双生,艾为党,赵成坚等.受控生态生保系统中植物生长光源的选择.航天医学与医学工程,2003,16:490~493.
    洪宇,童哲.光敏色素在植物个体发育中的作用[J].植物生理学通讯,1998,34(6):417~422.
    黄辰,桂美祥.光、Ca+2和CaM对菜豆叶枕外植体脱落的影响[J].植物生理学通讯,1991,27(5):347~350.
    黄少白,戴秋杰,刘晓忠.紫外光B辐射增强对水稻叶片内IAA和ABA含量的影响[J].植物学通报,1998,15:87~90.
    李春琳,张甜.芽苗类蔬菜的发展动态及其展望[J].长江蔬菜,2002,12:27~29.
    李德全,赵会杰,高辉远.植物生理学[M].中国农业科技出版社,1999.12:168~169.
    李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
    李韶山,潘瑞炽.蓝光对水稻幼苗生长效应的研究[J].中国水稻科学,1994,8(2):115~118.
    李韶山,潘瑞炽.蓝光对水稻幼苗碳水化合物和蛋白质代谢的调节[J].植物生理学报,1995,21(1):22~28.
    李韶山,潘瑞炽.植物的蓝光效应[J].植物生理学通讯,1993,29(4):248~252.
    刘成梅,游海.天然产物有效成分离与应用[M].北京:化学工业出版社,2003.181,201,202.
    刘明,赵琦,王小菁,等.植物的光受体及其调控机制的研究[J].生物学通报,2005,40(5):10~12.
    刘永丽.人工光源在闭锁式植物工厂中的应用研究[D].中国农业科学院,2007.
    陆新华.光照和温度对萝卜芽质量与产量的影响[J].广西热带农业,2002,4:5~6.
    马光恕,廉华,闰明伟.不同覆盖材料对大棚内番茄生长发育的影响[J].吉林农业科学,2002,27(4):41~43.
    倪文.光对稻苗根系生长及其生理活性的影响[J].作物学报,1983,(3):199~204.
    蒲高斌,刘世琦,刘磊,等.不同光质对番茄幼苗生长和生理特性的影响[J].园艺学报,2005,32(3):420~425.
    齐连东,刘世琦,许莉,等.光质对菠菜草酸、单宁及硝酸盐积累效应的影响[J].农业工程学报,2007,23 (4):201~205.
    时向东,蔡恒,焦枫,等.光质对作物生长发育影响研究进展[J].中国农学通报,2008,24(6):226~230.
    史宏志,韩锦峰,张国显等.单色蓝光和红光对烟苗叶片生长和碳氮代谢的影响.河南农业大学学报,1998(3):258~262.
    史宏志,韩锦峰,管春云,等.红光和蓝光对烟叶生长碳氮代谢和品质的影响[J].作物学报,1999,25(2):215~220.
    孙非,曹悦群,刘立侠,等.不同光质下栽培人参硝酸还原酶(NR)活性和蛋白质含量的变化[J].生物物理学报,1993,9(1):153~157.
    唐仕荣,高兆建. LED对纤维素酶产生菌生长的影响[J].徐州工程学院学报,2005,20(5):22~24.
    童哲,赵玉锦,王台等.植物的光受体和光控发育研究[J].植物学报, 2000,42(2):111~115.
    童哲.光质纯度对幼苗光形态建成的影响[J].植物生理学通讯,1989,(2):28~31.
    王德槟,张德纯.芽苗菜及栽培技术[M].北京:中国农业大学出版, 1998.10.
    王国荣.光质对黄瓜种子萌发过程中过氧化物酶活性及蛋白含量的影响.上海农业学报,1991,7(4):17~20.
    王海鸥,李广安,认识照明LED.中国照明电器,2004(2):1~3.
    王曼,王小菁.蓝光、紫外光的受体及其对CHS表达诱导的研究[J].植物学通报,2002,19(3):265—271.
    王玉英.激光植物工厂的现状与未来展望[J].光机电信息,2005,1:8~13.
    魏灵玲,杨其长,刘永丽.LED在植物工厂中的研究现状与应用前景[J].中国农学通报,2007,23(11):408~411.
    魏胜林,王家保,李春保.蓝光和红光对菊花生长和开花的影响[J].园艺学报,1998,25(2):203~204.
    吴光林,张光伦,黄寿波,等.果树生态学[M].北京:中国农业出版社,1992:21~27.
    吴惧,徐锦堂.光质对灵芝纤维素酶多酚氧化酶及其灵芝产量的影响中草药[J].中草药,1990,25(2):76~78.
    吴毅明,徐师华.温室塑料棚环境管理[M].北京:北京农业出版社,1990:4~6,20~23.
    徐凯,郭延平,张上隆,等.不同光质膜对草莓果实品质的影响[J].园艺学报,2007,34(3):585~590.
    徐茂军,朱睦元,顾青.发芽大豆中异黄酮积累的光诱导作用研究[J].中国粮油学报,2003,18:74~77.
    徐茂军,朱睦元.光诱导对发芽大豆中半乳糖酸内酯脱氢酶活性和维生素C合成的影响[J].营养学报,2002,24 (2):212~214.
    许莉,刘世琦,齐连东,等.不同光质对叶用莴苣光合作用及叶绿素荧光的影响[J].中国农学通报,2007,23(1):96~100.
    余让才,潘瑞炽.蓝光对水稻幼苗生长及内源激素水平的影响[J].植物生理学报,1997,23(2):175~180.
    张建伟.芽苗菜及其在我省的生产现状和发展前景[J].河南农业科学,2003,5:44~45.
    张丕方,董崇褶,倪德祥,等.光质对五种不同生活型植物的器官发生和生长的影响[J].武汉植物学研究,1989,7(4):339~344.
    张瑞华.光质与生姜生长发育及光能利用特性的关系.山东农业大学博士毕业论文,2008.
    张泽岑,王能彬.光质对茶树花青素含量的影响[J].四川农业大学学报,2002,20(4):337~339.
    赵德修,李茂寅,邢建民等.光质、光强和光期对水母雪莲愈伤组织生长和黄酮生物合成的影响[J].植物生理学报,1999,25:127~132.
    赵世杰,史国安,董新纯.植物生理学实验指导[M].北京:中国农业科学技术出版社,2002.
    郑洁,胡美君,郭延平.光质对植物光合作用的调控及其机理[J].应用生态学报, 2008,19(7): 1619~1624.
    Anna B, AlicjaK. Effect of light quality on somatic em-bryogenes is in Hacinthus or ientalis L.‘Delft’s blue’.Biological Bulletin of Poznan, 2001,38: 103~107. Barta,D.J.,Tibbitts,T.W.,Bula,R.J. and Morrow,T.W.Evaluation of lighting-emitting diodes characteristics for a space-based plant ir-radiation source.Advances Space Res.1992,12:141~149.
    Behringer J F,Davies P J,Indole-3-acetic acid levels after phy-tochrome-mediated changes in the stem elongation rate of dark-and light-grown Pisum seedlings[J].Planta,1992,188:85~92. Briggs W R,Beckb C F,Cashmorec J M,et al.The phototropin family of photoreceptors.Plant Cell,2001,13:993~997.
    Buchanun.B.B(美).植物生物化学与分子生物学[M].瞿永嘉译.北京:科学出版社,2004.2:812~815.
    Butler W L, Norris KH, SiegelmanHW, Hendricks S B.Detection, assay, and preliminary purification of the pig-ment controlling photoresponsive development of plants,Proc NatlAcad Sci USA,1959,45:1703~1708.
    Campbell W H.Nitrate reductase biochemistry comes of age[J].Plant Physiol,1996,111:355~361.
    ChristieJM,JenkinsGI.DistinctuV-BanduV-A/blue lightsignal transduction pathways induce chalcone synthasegeneexpressioninArabidopsiscells[-J].Plant Cell,1996,8:l555~l567.
    Chu Z-X. Effect of different light quality on photosynthetic characteristics of cucumber leaves.Acta Botanica Sinica, 1999,41(8): 867~870.
    Cosgrove D J. Rapid suppresion of growth by blue light: Occurrence time coures and ral characteristics. Plant Physiol, 1981, 67:584.
    Fang,W.,R.C.Jao and D.H.Lee.Artificial lighting apparatus for young plants using light emitting diodes as light source.US patent no.:US 6474838 B2.2002.
    Frechilla S, TalbottLD, BogomolniRA,etal. Reversal of blue light-stimulated stomatal opening by green light.Plant& CellPhysiology, 2000,41: 171~176.
    Fukai,S.,Fujiwara,K.,Okzmoto,K.,Hasegawa,A.,Goi,M.Effects of red and blue lights on germination and protocorm growth of Calanthe satsuma. Lindleyana,1997,12:169~171.
    Gallagher S,Short TW,Ray PM,Pratt LH,BriggsWR(1988).Light-mediated changes in two Proteins found assoeiated with Plasma membrane fraetions from Pea stem sections.Proc Natl Aead Sci USA,85:8003~8007.
    GilibertoL,PerrottaG,PallaraP,WellerJL,FraserPD,BramleyPM,FioreA,TavazzaM,GiulianoG.ManiPulation of the blue light PhotorecePtor cry Ptoehrom 2 intomato affeets vegetative develoPment,floweringtime,and fruiranti oxidanteontent.Plant Physiol,2005,137:199~208.
    Huala E,Oeller P W,Liscum E,et al.Arabidopsis NPH1:A protein kinase with a putative redox-sensing domain.Science,1997,278:2120~2123.
    Izumi H,Ito T,Yoshida Y.Sugar and ascorbic acid contents of Satsum mandarin fruits harvested from exterior and interior canopyof trees during fruit development[J].J Japan Soc Hort Sci,1990,58(4):877~883.
    Kasperbauerm J.Strawberry yield over red versus black plastic much[J].Crop Sci,2000,40:171~174.
    Kim HH, GoinsGD, WheelerRM,etal. Stomatal con-ductance of lettuce grown under or exposed to different light qualities.Annals ofBotany, 2004,94: 691~697.
    Kowallik W. Blue light effects on respiration[J]. Annu Rev Plant Physiol, 1982, 33: 51~72
    Lam H M, Coschigano KT, Oliveira IC, et al. The molecular genetics of nitrogen assimilation into amino acids in higher pants[J]. Annu Rev Plant Physiol Plant Mol B iol, 1996, 47: 569~593.
    LeongTY, Goodchild DJ, Anderson JM. Effect of lightquality on the composition, function, and structure ofphotosynthetic thylakoid membranes of A splenium aus-tralasicum( Sm. ) Hook.Plant Biology, 1985,78:561~567.
    Lin C(2000).Plantblue-lightreeePtors.Trends Plant Sci,5:337~342.
    Meijer G. Some aspects of plant irradiation. Zcta Hortic. Intern. Soc. Hortic Sci, 1971, 22:103~108.
    Nick P, Furuay M. Phytochrome dependent decrease of gib-berellin-sensitinity[J].Plant Grow Regulation,1993,12:95~206.
    Ninnemann H.Some aspects of blue light research during last decade[J].Photochem Photobiol, 1995,61:22~31.
    Oba K,Ishikawa S,Nishikawa M,eta1.Purification and properties of L galactono-1,4-lactone dehydrogenase,a key enzyme for ascorbic acidbiosynthesis,from sweetpotato[J] .J Biochem,1995,117:120~124.
    Quail P H, Boylan MT, Parks B M, Short T W, Xu Y,Wagner D. Phytochromes: Photosensory perception and sig-nal transduction.Science,1995,268:675~680.
    Ramalho JC, Marques NC, Semedo JN,et al. Photo-synthetic performance and pigment composition of leavesfrom two tropical species is determined by light quality.Plant Biology, 2002,4: 112~120.
    Reed J W,Nagpal P,Poole D S.Mutations in the gene for the red/far-red light receptor Phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development.Plant Cell,1993,5:147~157.
    Rivkin RB. Influence of irradiance and spectral qualityon the carbon metabolism of phytoplankton. I. Photo-synthesis, chemical composition and growth.Marine Ecology Progress Series, 1989,55: 291~304.
    Saebo A, Krekling T, Appelgren M. Light quality af-fects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell, Tissue and Organ Culture, 1995,41: 177~185.
    Seihert M,Welherbee P J,Job D D.Plant Physiol[M],1975,56:130~l39.
    ShalitinD,YangH,Moekler TC,MaymonM,Guo H,Whitelam GC,LinC(2002).Regulation of Arabidops cryPtochrome2 by blue-light-dePendent phosphorylation..Nature,417:763~767.
    Shropshive W Jr, Mohr H. Photomorphogenesis: Encyclo-pedia of Plant Physiology, New Series, Vol. 16A, B.Berlin: Springer Verlag, 1983.
    Smith K C.光生物学.北京.科学出版社, 1984.
    Sun Q,Yoda K,Suzuki H.Internal axial light conduction in the stems and roots of herbaceous plants[J].J Exp Bot,2005,56:191~203.
    Swart T E,Corchnoy S B,Christie J M,et al.The photocycle of a falvin-binding domain of the blue light photoreceptor phototropin.Journal Biology Chemistry 2001,276.
    TalbottLD, ZeigerE. Sugar and organic acid accumula-tion in guard cells ofVicia fabain response to red and blue light.PlantPhysiology, 1993,102: 1163~1169.
    Toledo MEA,Ueda Y,Imahori Y,et al.L-ascorbic acid metabolism in spinach(Spinacia Oleracea L.)during postharvest storage in light and dark[J].Postharvest Biology and Technology,2003,28(1):47~57.
    Tong Z,Kasemir H,Mohr H.Coaetian of light and cytokinin in photo morphogenesis[J].Plnama,1983,159:136~142.
    Tullio MCD,Ciraci S,Liso R,et al.Asorbic acid oxidase is dynamically regulated by light and oxygen.A tool for oxygen management in plant [J].Plant Physiology,In press.corrected proof Availiable online 15 December 2005:42~54.
    Vlasova M P,Drozdova S,Voskresenskaya N P.Modification of chloroplast ultrastructure in pea plants greening under blue and red light.Fiziol.Rast.(USSR),1971,18:5~11.
    Voskresenskaya NP, Drozdova IS, Krendeleva TE. Effect of light quality on the organization of photosynthetic electron transport chain of pea seedlings[J]. Plant Physiol , 1977, 59:151.
    Yamaguchi S, Smith MW, Brown R G, Kamiya Y, Sun TP. Phytochrome regulation and differential expression of gib-berellin 3β-hydroxylase genes in germinatingArabidopsisseeds.Plant Cell,1998,10:2115~2126.
    Yanagi T., K. Okamoto and S. Takita. Super-bright light emitting diodes as an artificial light source for plant growth[J]. Abstract of 3rd International Symposium on Artificial Lighting in Horticulture, 1994, 19.
    Yu R C, Pan R C. Effect of blue light on the respiration of rice (Oryza sativa) seedlings.Chin J Rice Sci,1996,10:159~162.
    Zeiger E. The biology of stomatal guard cells.Annual Review ofPlantPhysiology, 1983,34: 441~475.

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