紫锥菊提取物对鲤生产性能和免疫功能的影响
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摘要
为探明紫锥菊提取物对鲤鱼抗病促生长作用,以240尾鲤鱼作为实验动物,在其日粮中按0mg/kg、1000mg/kg、2000mg/kg和4000mg/kg的不同比例添加紫锥菊提取物,经过30d的饲养,测定鲤鱼的相对生长率、增重、饵料系数、摄食量和成活率,在生长实验完成后进行攻毒实验,测定其保护率,确定紫锥菊提取物对鲤鱼生长性能和抗病力的影响。
在饲料中分别添加0mg/kg、1000mg/kg、2000mg/kg和4000mg/kg的紫锥菊提取物投喂480尾鲤鱼35d,分别在第7d、第14d、第21d、第28d和35d测定鲤鱼血清溶菌酶,血清总蛋白,血清白蛋白,白细胞吞噬率和血清中的免疫球蛋白(IgM、IgG和IgA);35d后分别测定紫锥菊提取物对鲤鱼血清、肾脏、肝脏和肌肉中的AKP、ACP、SOD和MDA的影响。生长实验结果显示:各试验处理组除对鲤鱼成活率和摄食量的影响不大外,对增重、饵料系数和相对生长率都有不同程度的作用。免疫结果表明:血清总蛋白、白蛋白、溶菌酶、白细胞吞噬率和吞噬指数均是在21d或28d后与对照组相比才出现明显的变化,其中试验1组(1000mg/kg)和试验2组(2000mg/kg)的血清总蛋白含量在第21d较对照组有所升高;28d后吞噬率较对照组显著提高(P＜0.01),而吞噬指数在不同时间与对照组相比呈现出不同的显著水平;就血清中免疫球蛋白IgA而言,在整个试验周期内各个试验组与对照组之间均未出现明显差异,而且在35d后,试验3组(4000mg/kg组)的含量还低于对照组(P＞0.05)。而IgM的含量仅在28d和35天后试验1组(1000mg/kg)和2组(2000mg/kg)极显著高于对照组(P＜0.01)。试验2组的IgG水平(2000mg/kg)分别在试验21d和35d开始有明显变化,且分别达到显著(P＜0.05)和极显著水平(P＜0.01)。在未添加紫锥菊提取物的对照组,血清、肌肉、肝脏和肾脏中的AKP、ACP、SOD的活力各不相同;添加紫锥菊提取物后,对组织中各项酶活力的影响各异,各个试验组对肌肉和肾脏中AKP酶活力均较对照组极显著升高(P＜0.01),2000mg/kg组肝脏中AKP酶活力较对照组极显著升高(P＜0.01),血清中只有2000mg/kg组的AKP酶活力显著高于对照组(P＜0.01);肾脏和血清中仅2000mg/kg组的ACP的酶活力较对照组显著升高(P＜0.01),其它两个剂量组与对照组相比差异没有显著变化(P＞0.05),而肌肉中各个处理组的ACP酶活力与对照组相比显著增加(P＜0.01);血清和肌肉中仅见4000mg/kg组的SOD活力的升高水平与对照组达到显著差异(P＜0.01),肝脏中的各个实验组的SOD活力均高于对照组(P＜0.01),肾脏中的2000mg/kg和4000mg/kg两个剂量组的SOD活力与对照组相比显著提高(P＜0.01);在血清中2000mg/kg和4000mg/kg剂量组MDA的含量与对照组相比显著下降(P＜0.01),肝脏中仅见2000mg/kg组MDA的含量较对照组显著下降(P＜0.01),肾脏中2000mg/kg和4000mg/kg两个剂量组的MDA含量显著下降(P＜0.01),而肌肉中的各个实验组的MDA的含量均低于对照组(P＜0.01)。攻毒实验表明,紫锥菊提取物能有效增强鲤鱼对嗜水气单胞菌的抵抗能力,各个实验组相比对照组显示出良好的免疫保护作用。
通过本次试验结果可以证实,紫锥菊提取物能有效的提高鲤鱼生长性能、免疫功能和对嗜水气单胞菌的抵抗能力。因此,紫锥菊提取物可作为一种优良的中草药免疫增强剂应用于养殖中,建议添加剂量(有效成分5%)为2000mg/kg左右。
Cyprinus carpio L(carp) were used for examining the echinacea purpurea extract as a feed additive on their performances effect.Two hundred and fourty carps were randomly individed into 4 groups.In this 4 groups,the one is control,the group 1、group 2 and group 3 are supplied with 0 mg/kg(control) Echinacea purpurea extract、1000mg/kg Echinacea purpurea extract,2000mg/kg Echinaceapurpurea extract and 4000mg/kg Echinacea purpurea extract.At the end of 30-d feeding trial,determine the relative growth rate(RGR)、adding-weight、feed coefficient rate(FCR)、ingestion apprtite and survival rate.Aider the performance trial examined carp resistance against the infection from Aeromonas hydrophila.
To determine the effects of echinacea purpurea extract on the innate immune response of Cyprinus carpio L(carp),fish were fed diets containing 0(control)、1000mg/kg,2000mg/kg and 4000mg/kg Echinaceapurpurea extract,for 7,14,21,28 and 35days.Lysozyme,blood leucocyte phagocytica ctivity,serum albumin,serum total protein and serum immunoglobulin (including IgM,IgG and IgA) were studied at each of the assay times.After 40 days,the AKP、ACP、SOD and MDA in tissues including serum、liver、kinder and muscel were detected.The results showed that the survival rate and FCR had no enhancement of every group in performance trial,meanwhile the Lysozyme,blood leucocyte phagocytica ctivity,serum albumin,serum total protein were variation after 21 or 28 days;immunoglobulin IgA had no change in whole trial cycle,the contents of IgM had significant difference(P＜0.01) after 28 and 35 days and IgG of 2000mg/kg group had significant difference in 21 and 35 days.Compared with the control group,the echinacea purpurea extract had a good effect for the AKP、ACP、SOD activitys and MDA contents.The infection trial result showed the echinacea purpurea extract had a good effect on the carp resistance against the infection from Aeromonas hydrophila.
The trial results indicated echinacea purpurea extract could enhanced the growth performance and anti-diseases effect.It is a superordinary additive on aquatic.For the immunomodulatory efect of echinaceapurpurea extract and take the economy and efficiency into account,it is suggested that the feasible dosage was about 2000mg/kg.
在饲料中分别添加0mg/kg、1000mg/kg、2000mg/kg和4000mg/kg的紫锥菊提取物投喂480尾鲤鱼35d,分别在第7d、第14d、第21d、第28d和35d测定鲤鱼血清溶菌酶,血清总蛋白,血清白蛋白,白细胞吞噬率和血清中的免疫球蛋白(IgM、IgG和IgA);35d后分别测定紫锥菊提取物对鲤鱼血清、肾脏、肝脏和肌肉中的AKP、ACP、SOD和MDA的影响。生长实验结果显示:各试验处理组除对鲤鱼成活率和摄食量的影响不大外,对增重、饵料系数和相对生长率都有不同程度的作用。免疫结果表明:血清总蛋白、白蛋白、溶菌酶、白细胞吞噬率和吞噬指数均是在21d或28d后与对照组相比才出现明显的变化,其中试验1组(1000mg/kg)和试验2组(2000mg/kg)的血清总蛋白含量在第21d较对照组有所升高;28d后吞噬率较对照组显著提高(P＜0.01),而吞噬指数在不同时间与对照组相比呈现出不同的显著水平;就血清中免疫球蛋白IgA而言,在整个试验周期内各个试验组与对照组之间均未出现明显差异,而且在35d后,试验3组(4000mg/kg组)的含量还低于对照组(P＞0.05)。而IgM的含量仅在28d和35天后试验1组(1000mg/kg)和2组(2000mg/kg)极显著高于对照组(P＜0.01)。试验2组的IgG水平(2000mg/kg)分别在试验21d和35d开始有明显变化,且分别达到显著(P＜0.05)和极显著水平(P＜0.01)。在未添加紫锥菊提取物的对照组,血清、肌肉、肝脏和肾脏中的AKP、ACP、SOD的活力各不相同;添加紫锥菊提取物后,对组织中各项酶活力的影响各异,各个试验组对肌肉和肾脏中AKP酶活力均较对照组极显著升高(P＜0.01),2000mg/kg组肝脏中AKP酶活力较对照组极显著升高(P＜0.01),血清中只有2000mg/kg组的AKP酶活力显著高于对照组(P＜0.01);肾脏和血清中仅2000mg/kg组的ACP的酶活力较对照组显著升高(P＜0.01),其它两个剂量组与对照组相比差异没有显著变化(P＞0.05),而肌肉中各个处理组的ACP酶活力与对照组相比显著增加(P＜0.01);血清和肌肉中仅见4000mg/kg组的SOD活力的升高水平与对照组达到显著差异(P＜0.01),肝脏中的各个实验组的SOD活力均高于对照组(P＜0.01),肾脏中的2000mg/kg和4000mg/kg两个剂量组的SOD活力与对照组相比显著提高(P＜0.01);在血清中2000mg/kg和4000mg/kg剂量组MDA的含量与对照组相比显著下降(P＜0.01),肝脏中仅见2000mg/kg组MDA的含量较对照组显著下降(P＜0.01),肾脏中2000mg/kg和4000mg/kg两个剂量组的MDA含量显著下降(P＜0.01),而肌肉中的各个实验组的MDA的含量均低于对照组(P＜0.01)。攻毒实验表明,紫锥菊提取物能有效增强鲤鱼对嗜水气单胞菌的抵抗能力,各个实验组相比对照组显示出良好的免疫保护作用。
通过本次试验结果可以证实,紫锥菊提取物能有效的提高鲤鱼生长性能、免疫功能和对嗜水气单胞菌的抵抗能力。因此,紫锥菊提取物可作为一种优良的中草药免疫增强剂应用于养殖中,建议添加剂量(有效成分5%)为2000mg/kg左右。
Cyprinus carpio L(carp) were used for examining the echinacea purpurea extract as a feed additive on their performances effect.Two hundred and fourty carps were randomly individed into 4 groups.In this 4 groups,the one is control,the group 1、group 2 and group 3 are supplied with 0 mg/kg(control) Echinacea purpurea extract、1000mg/kg Echinacea purpurea extract,2000mg/kg Echinaceapurpurea extract and 4000mg/kg Echinacea purpurea extract.At the end of 30-d feeding trial,determine the relative growth rate(RGR)、adding-weight、feed coefficient rate(FCR)、ingestion apprtite and survival rate.Aider the performance trial examined carp resistance against the infection from Aeromonas hydrophila.
To determine the effects of echinacea purpurea extract on the innate immune response of Cyprinus carpio L(carp),fish were fed diets containing 0(control)、1000mg/kg,2000mg/kg and 4000mg/kg Echinaceapurpurea extract,for 7,14,21,28 and 35days.Lysozyme,blood leucocyte phagocytica ctivity,serum albumin,serum total protein and serum immunoglobulin (including IgM,IgG and IgA) were studied at each of the assay times.After 40 days,the AKP、ACP、SOD and MDA in tissues including serum、liver、kinder and muscel were detected.The results showed that the survival rate and FCR had no enhancement of every group in performance trial,meanwhile the Lysozyme,blood leucocyte phagocytica ctivity,serum albumin,serum total protein were variation after 21 or 28 days;immunoglobulin IgA had no change in whole trial cycle,the contents of IgM had significant difference(P＜0.01) after 28 and 35 days and IgG of 2000mg/kg group had significant difference in 21 and 35 days.Compared with the control group,the echinacea purpurea extract had a good effect for the AKP、ACP、SOD activitys and MDA contents.The infection trial result showed the echinacea purpurea extract had a good effect on the carp resistance against the infection from Aeromonas hydrophila.
The trial results indicated echinacea purpurea extract could enhanced the growth performance and anti-diseases effect.It is a superordinary additive on aquatic.For the immunomodulatory efect of echinaceapurpurea extract and take the economy and efficiency into account,it is suggested that the feasible dosage was about 2000mg/kg.
引文
[1].刘一兵.(2001).紫锥菊属植物制剂的化学、免疫作用与临床[J].国外医药·植物药分册.16(2):47-54;
[2].尹进,彭芝配,马建中等.(2006).松黄颗粒对小鼠单核-巨噬细胞碳廓清及其抗体生成细胞的影响[J].湖南中医学院学报.26(2):10-11;
[3].林清华,(1999).免疫学实验[M].武汉大学出版社.64-67;54-56;
[4].陈昌福,罗宇良,蔡冰.(1996).实验室水温对草鱼溶菌酶活性的影响[J].中国水产科学,3(3):24-30;
[5].王军.(2001).免疫添加物对大黄鱼血液白细胞数量及吞噬功能的影响[J].海洋科学,25(9):17-19;
[6].秦启伟.(2000).饵料维生素C对青石斑鱼的非特异性免疫调节作用[J].热带海洋,19(1):58-63;
[7].孙德文,张艳秋,黄磊.(2004).鱼类免疫增强剂的研究进展[J].江西饲料,(2):19-23;
[8].江晓路,刘树青,张朝辉等.(1999).多糖对中国对虾免疫功能的影响[J].中国水产科学,6(1):66-68;
[9].秦大莲.(2006).药理学实验指导[M].414-415;
[10].王康才,唐晓清,朱光明,(2000).紫锥菊属植物的化学成分、药理及开发利用研究进展[J].中药材23(12):780-782;
[11].李玮,郭腾,陈洪亮等.(2006).紫锥菊研究进展及在动物保健上的应用展望[J].兽药与饲料添加剂,11(3):28-30;
[12].吴江涛,刘珂.(2001).紫锥菊提取物对小鼠单核巨噬细胞系统功能的影响[J].烟台大学学报(自然科学与工程版)14(2):131;
[13].张英涛,刘文芝.(2002).松果菊属3种药用植物的DNA分子鉴别研究[J].中国中医药信息杂志9(5):11-12;
[14].刘树青,江晓路,牟海津等.(1999).免疫多糖对中国对虾血清溶菌酶、磷酸酶和过氧化物酶的作用[J]_海洋与湖沼,30(3):278-283;
[15].江晓路,刘树青,张朝晖等.(1999).多糖对中国对虾免疫功能的影响[J].中国水产科学,6(1):66-68;
[16].邵健忠,项黎新.(2001).草鱼干扰素的免疫调节功能[J].动物学报,47(4):404-411;
[17].崔青曼,张耀红.(2001).中草药、多糖复合添加剂提高河蟹机体免疫力的研究[J].水利渔业,21(4):40-41;
[18].陈昌福,曾妍雄,楠田理一.(2000).接种不同种类病原菌脂多糖对翘嘴鳜细菌性败血症的免疫保护力比较[J].华中农业大学学报[J].19(4):377-379;
[19].赵红霞,詹勇,许梓荣.(2001).水产动物免疫增强剂的研究[J],饲料研究,8,1-4;
[20].江晓路,刘树青.(1999).真菌多糖对中国对虾血清及淋巴细胞免疫活性的影响[J],动物学研究,20(1):41-43;
[21].王吉桥,徐锟,(2002).对虾对营养物质的需要量[J],大连水产学院学报,17(3):196-206;
[22].王新霞,谭北平,麦康森等,(2004).β-葡聚糖在中国对虾(Penaeus chinensis)育苗中的应用[J],高技术通讯,4,71-75;
[23].谭北平,周歧存,郑石轩等,(2004).β(1,3)/(1,6)-葡聚糖葡聚糖制剂对凡纳对虾生长及免疫力的影响[J],高技术通讯,.5,73-77;
[24].汪小锋樊廷俊丛日山等,(2005).几种免疫促进剂对中国对虾血细胞数量、形态结构以及酚氧化酶产量和活性的影响[J].水产学报 29(1):66-73;
[25].陈超然,陈昌福,(2001).鱼用疫苗的研究现状[J].水利渔业,21(5):44-45;
[26].刘云,孙峰,王丹.(2004),免疫增强剂对鲫鱼非特异性免疫功能的影响[J],海洋科学,28(9):42-45;
[27].池雪林,吴德峰,(2004).浅谈中草药免疫增强剂研究进展[J].福建畜牧兽医,26(4):12-13;
[28].孙志良,陈立祥,章怀云等.(1999)α-干扰素对中国对虾皮下及造血组织坏死杆状病毒的作用[J].湖南农业大学学报,25(2):131-132;
[29].冯蓓莉,(2002),陆森泉.乳铁蛋白的抗病毒作用[J].国外医学:微生物学分册,25(1):5-6,10;
[30].孙德文,张艳秋,黄磊.(2004).鱼类免疫增强剂的研究进展[J],江西饲料,(2):19-23;
[31].赵红霞,杨建军.(2003)免疫增强剂在水产养殖的研究进展[J],江西饲料,(4):18-21;
[32].张国辉,何瑞国,张世萍等.(2007)维生素E对黄鳝繁殖性能的影响[J].水生生物学报.25(2):177-18l;
[33].张耀武,屈文俊,李文辉.(2006)β1,3-葡聚糖对锦鲤非特异性免疫功能的影响[J].淡水渔业.第36卷第4期 53-55:
[34].王宏田,徐永立,张培军.(2000).假雄牙鲆不同组织中溶菌酶比活性的研究[J].海洋科学.24(10):7-8;
[35].王远吉.(2004).枸杞多糖对鲫鱼血清溶菌酶活性的影响[J].中国饲料.24.28-31;
[36].丁磊,黄鹤忠,吴康等.(2004).镉对鲫非特异性免疫力的影响[J].农业环境科学学报.23(1):64-66;
[37].何四旺,许国焕,吴月嫦等.(2003).低聚异麦芽糖和低聚果糖对罗非鱼生长和非特异性免疫的影响[J].中国饲料.(23):14-15;
[38].刘华忠,刘定忠,赵学明.(2004).复方中草药对彭泽螂非特异性免疫功能的影响[J].淡水渔业.34(3):31-32;
[39].刘勇,贾永红.(2006).免疫增强剂对河鲫鱼白细胞吞噬和溶菌酶活性的影响[J].安徽农业科学_35(1):111-113:
[40].罗庆华.(2002).杜仲叶粉对鲤鱼免疫力的影响[J].湖南农业大学学报.28(1):51-53;
[41].王鹏,江晓路,江艳华等(2006).褐藻低聚糖对提高大菱鲆免疫机能的作用[J].海洋科学30(8):6-9;
[42].张梁,沈建忠,敖茂权等.(2006)蛭弧菌对草鱼免疫相关酶活性的影响[J].江西农业大学学报.28(6):295-299;
[43].张梁,张伟.任小丽(2007).光合细菌对草鱼生长及免疫相关酶活性的影响[J].粮食与饲料工业 (2):37-38;
[44].沈文英,阳会军,柯慧芬.(2007).β-葡聚糖对凡纳滨对虾免疫相关酶活性的影响[J].水产科学.26(7):381-383:
[45].谢玉霖,王寿昆,辛志明等,(2007).中草药在水产动物饲料添加剂上的应用[J].福建农业科学,(3):63-65;
[46].管华诗.(1999).海水养殖动物的免疫、细胞培养和病害研究[M].济南:山东科学技术出版社,23-24;
[47].姜蕾,郑曙明(2006),五倍子对鲤鱼细菌性败血症的药效实验初报[J].水产渔业科学.22(5):460-464;
[48].叶榕,张琪瑛,杨奇伟等.(1995).低浓度混苯对作业者血浆SOD的影响[J].中国工业医学杂志,8(4):206-207;
[49].宋善俊.(1991).临床医师手册[M].上海:上海科学技术出版社,185-200;
[50].林林等,(1998)有机污染提高对虾对病原菌敏感性实验[J].海洋学报.20(1):90-93;
[51].陈昌福,罗宇良,蔡冰.(1996).实验水温对草鱼溶菌酶活性的影响[J].中国水产科学,3(3):24-30;
[52].周歧存,丁炳,郑石轩等.(2004).维生素C对凡纳滨对虾生长及抗病力的影响[J].水生生物学报,6:592-598;
[53].李明,董晓慧.(2007)中草药在水产养殖中的研究进展[J].(2):饲料工业.56-58;
[54].杨严鸥,姚峰,吴继鹏,刘涛.(2005).温度对黄颡鱼血细胞数量、大小及血清生化成分的影响[J].长江大学学报.2(11):58-63;
[55].王文辉,王吉桥,程鑫等.(2006).不同剂型维生素C对生长和几种免疫指标的影响[J].中国水产科学.13(6):951-957;
[56].朱忠勇.(1997).实用医学检验学[M].北京:人民军医出版社.368-378;
[57].陈瑛,邱子健,隋淑光等.(2003).棘尾虫酸性磷酸酶的定位及诱导表达[J].动物学报,49(2):218-223;
[58].魏炜,张洪渊,石安静.(2001).育珠蚌酸性磷酸酶活力与免疫反应关系的研究[J].水生生物学报,25(4):413-415;
[59].陈国福,宋晓玲,黄健等.(2007).A3_((α))-肽聚糖对凡纳滨对虾磷酸酶及细胞内酚氧化酶活性的影响[J].海洋水产研究.28(1):50-64;
[60].樊甄姣,刘志鸿,杨爱国等.(2006).Vc对栉孔扇贝体内水解酶和抗氧化酶活性的影响[J].海洋水产研究.27(4):12-16;
[61].王学高,白冰,李西汉等.(2006)灰树花多糖对鲫鱼免疫功能的影响[J].水产科学.25(1):9-12;
[62].王秀华.(2003).肽聚糖对对虾免疫因子的作用及其在对虾养殖中的应用[D].中国海洋大学硕士学位论文18-47;
[63].王宏田,张培军.(2000).重组酵母菌对牙鲆非特异性免疫能力的影响[J].海洋与湖沼.31(6):631-633;
[64].郝林华,孙玉喜,石红旗等.(2007).牛蒂寡糖对大菱鲆生长和免疫机能的影响[J].海洋科学进展.25(2):208-2 14;
[65].马贵华,刘六英,钟青等.(2006).中华绒鳌蟹超氧化物歧化酶的初步研究[J].水利渔业.24(4):24-25;
[66].孙焕顷.(2005)鲤鱼磷酸碱性酶(AKP)的分离纯化[J].衡水学院学报,1(3):32-35;
[67].陈清西,陈素丽,石艳等.(1996).长毛对虾碱性磷酸酶的性质[J].厦门大学学报(自然科学版),35(2):257-261;
[68].陈志伟,贾秀英.(2002).氟对家蚕血液及中肠磷酸酶活性的影响[J].农业环境保护,21(2):130-133;
[69].张红梅.(2006).甘露寡糖对鲤鱼血液生化指标的影响[J].饲料研究.(1):39-41;
[70].刘红柏,卢彤岩,张春燕等.(2006).黄芪对史氏鲟抗氧化能力及免疫力的影响[J].大连水产学院学报 2(3):231-235;
[71].罗璋,姚鹃,陈昌福等.(2007).酵母免疫多糖对受免斑点叉尾鲴免疫应答的增强作用[J].淡水渔业.37(3).2-25;
[72].李友光.(1995).中国对虾疾病与免疫机制[J].海洋科学.(4):1-3;
[73].陈缓,周玫.(1991).自由基医学[M].北京:人民军医出版社,:30-31;
[74].王丽辉.(2007).中草药与益生素对断奶仔猪生长和免疫作用的影响[J].福建畜牧兽医.29(3):21-23;
[75].张琼.(2004).维生素E对幼建鲤生产性能和免疫功能的影响[D].四川农业大学硕士论文;
[76].刘清海,冯静,李健.(2000).微生物多肽对黑鲷免疫和生长的促进作用[J].海洋科学.24(5):11-13;
[77].赵远,和占龙,陈丽雄等.(2007).日粮中添加苜草素对幼猴免疫球蛋白IgG、IgA、IgM的影响[J].实验动物科学.24(3):33-35;
[78].杨严鸥,姚峰,吴继鹏等.(2005)温度对黄颡鱼血细胞数量、大小及血清生化成分的影响[J].长江大学学报.2(11):58-59;
[79].周进.(2004).免疫增强剂-A3a肽聚糖对牙鲆免疫功能及抗病力的影响[D].上海水产大学硕士论文:
[80].刘勇,杨季芳,陈昌福.(2007).免疫增强剂对河鲫鱼白细胞吞噬和溶菌酶活性的影响[J].(2):11-15;
[81].姚红梅.(2005).杜仲提取对彭泽鲫抗病和促生长研究[D].湖南农业大学硕士论文;
[82].朱晓,徐祝封,陈振武.(1997).中药理化鉴定[M].北京:中国中医药出版社;
[83].A.Cuesta,J.Meseguer and M.A.Esteban,(2004),Total serum immunoglobulin M levels are affected by immunomodulators in seabream(Sparus aurata L.)[J],Veterinary Immunology and Immunopathology101:203-210;
[84].Anderson,D.P,Siwicki,A.K.(1995).Injection or immersion delivery of selected immunostimulants to trout demonstrate enhancement of nonspecific defense mechanisms and protective immunity[J].Diseases in Asian Aquaculture,11:413-426;
[85].Anderson,D.P,(1996).Environmental factors in fish health:Immunological aspects.In the fish immune system.Organism,pathogen,and environment(Glwama&T.Nakanishi.eds)289-310.London:Academic press;
[86].Alberto Cuesta,Alejandro Rodriguez,Irene Salinas,et al.(2007).Early local and systemic innate immune responses in the teleost gilthead seabream after intraperitoneal injection of whole yeast cells[J].Fish & Shellfish Immunology.22,(3):242-251;
[87].Bany J,Siwicki AK.(2003).Echinacea purpurea stimulates cellular immunity and anti-bacterial defence independently of the strain of mice[J].Pol J Vet Sci,3(6):3-5;
[88].Berczi I.,Nagy E.(1987).The effect of prolactin and growth hormone on hemolymphopoietic tissue and immune function[M].Hormone and lmmunity,MTP Press,Lancaster,.145-147;
[89].Binns S E,Livesey J F,Arnason J T.(2002).Phytochemical variation in Echinacea from roots and flowerheads of wild and cultivated populations[J],J.Agric.Food Chem,50(13):3673-3687;
[90].Bodinet C,Mentel R.(2002).Effect of oral application of an immunomodulating plant extract on influenza virus type A infection in mice[J].Planta Med,68(10):896-900;
[91].Currier N L,Miller S C.(2000).Natural killer cells from aging mice treated with extracts from Echinacea purpurea are quantitatively and functionally rejuvenated[J].Exp Gerontol,.35(5):627-639;
[92].Chen Changfu,Li Jing,Riichi Kusuda.(1996).A preliminary study on the adjuvanticity of henbane in oral vaccination of grass carp(Ctenopharyngodon idellus)[J].华中农业大学学报,15(2):157-162;
[93].Cuesta,A.,Ortuno,J.,Podriguze,M.A.,et al,(2002).Changes in some defences par am ete rso fs eabream(Sparesa yrataL.)induced by retinol acetate.Fish&Shellfish Immunology.13,279-291;
[94].Cook,M.T,Haybill,P.J.,Hunchinson,et al,(2003).Administration of a commercial immunostimulant perparation,EcoActiva as a feed supplement enhances macrophage respiratory burst and the growth rate of snapper(Pagrus aratus,Sparidae(Bloch and Schneider))in winter.Fish&Shellfish Immunology.14,333-345;
[95].Chuntao Yuan,Dongmei Li,Wei Chen et al.(2007).Administration of a herbal immunoregulation mixture enhances some immune parameters in carp(Cyprinus carpio )[J].Fish physiology and biochemistry33.93-101;
[96].Chen S-C,Yoshida T,Adams A,et al.(1998) Non-specific immune response of Nile tilapia,Oreochronis nilotica,to the extracellular productsof Mycobacterium spp and to various adjuvants.Journal of Fish Diseases,21:39-46;D.Marionnet,P.Deschaux and S.Reynaud.(2006).Possible implication of macrophages in the regulation of cytochrome P450 activities in carp(Cyprinus carpio)[J].Fish & Shellfish Immunology,21(1):80-91;
[97].Esteban M A,Mulem V,Cuesta A et al.(2000).Effect of injecting chitin particles on the innate immune response of gilthead seabream(Spares aurata L)[J].Fish and Shellfish Immunology,10:543-554;
[98].Eduardo M.Leano,Jiin-Ju Guo et al.(2003).Levamisole Enhances Non-Specific Immune Response of Cobia,Rachycentron canadum,Fingerlings[J].J.Fish.Soc.Taiwan.30(4):321-330;
[99].Fsteban M A,Cuesta A,Ortuno J et al.(2001).Immunomodulatory effects of dietary intake of Chitin on gilthead seabream(Spares aurata L.)innate immune system[J].Fish and Shellfish Immunology,11:303-315;
[100].Freier DO,Wright K.(2003).Enhancement of the humoral immune response by Echinacea purpurea in female Swiss mice[J].lmmunopharmacol lmmunotoxicol,25(4):551-560;
[101].Guanghong Wu,ChuntaoYuan,Meifang Shen.et al.(2007).Immunological and biochemical parameters in carp(Cyprinus carpio) after Qompsell feed ingredients for long-term administration[J].38(3),246-255;
[102].Galindo-Villegas Jorge et al,(2006).Effect of Continuous and Interval Administration of Peptidoglycan on Innate Immune Response and Disease Resistance in Japanese Flounder Paralichthys olivaceus[J].Suisan Zoshoku.54;(2):163-170;
[103].Galindo-Villegas Jorge Fukada Haruhisa.Masumoto Toshiro et al.(2006 ).Effect of Dietary Immunostimulants on Some Innate Immune Responses and Disease Resistance against Edwardsiella tarda Infection in Japanese Flounder(Paralichthys olivaceus)[J].54(2):153-162;
[104].Hironobu Watanuki,Kazuki Ota,Asmi Citra Malina A.R.et al.(2006).Immunostimulant effects of dietary Spirulinaplatensis on carp,Cyprinus carpio[J].Aquaculture 258:157-163;
[105].Hou Ya Yi.(2001).Effects of estradiol-17β on immunocompetence in rainbow trout(on corhynchus mykiss)[J].Aeta Zoologica sinica 47(3):258-291;
[106].Itami T.,Kondo M.,Uozu M..et ai.(1996).Ehancement of disease resistance against Enterococcus seriolicida infection in yellowtail Seriola quinqueradiata Temminck and Schlegel by oral administration of Peptidoglycan derived from Bifidobacterim thermophilum[J].J.Fish Dis.,19:185-187;
[107].Itami Toshiaki,Masaya Asano.(1998).Enhancement of disease resistance of kuruma shrim o,penaeus japonicus after oral administration of peptidoglican derived from Bifidobacterium thermopilum[J].Aquaculture,164:277-288;
[108].J.Skjermo,Q.Bergh.(2004).High-Malginateimmunostimulation of Atlantic halibut(Hippoglossus hippoglossus L.) larvae using Artemia for delivery,increases resistance against vibriosis[J].Aquaculture,238:107-113;
[109].Jaya Kumari and P.K.Sahoo,(2006).Non-specific immune response of healthy and immunocompromised Asian catfish(Clarias batrachus) to several immunostimulants[J],Aquaculture255:133-141;
[110].John Paul.(2007).The influence of microcystin-LR on fish phagocytic cells[J].Human & Experimental Toxicology,26(7):603-607;
[111].Kajita,Y,Sakas,M,Atsuta,S.(1992).Immunopotentiantion Activety of Freund's complete adjuvant in rainbow trout[J],Oncorhynchus mykiss.Fish Shellfish lmmunol,2:155-157;
[112].Kajita Y.(1996).Erthancement of non-specific cytotoxic activity of leucocytes in rainbow trout Oncorhynchus mykiss injected with exogenous growth hormone[J].Fish&Shellfish Immunology,(6):615-617;
[123].Kakuta,I.,Kurokura,H.(1996).Defensive effect of orally administered bovine lactoferrin against Cryptoicaryon irritants infection of red seabream[J].Fish Pothlogy,30:289-290;
[114].Kawakami H.,Dosako S.(1998).The non-specific immunostimulation and adjuvant effects of Vibrio anguillarum bacterin,M-glucan,chitin or Freund's complete adjuvant in yellow seripla quinqueradiata to Pasteurella piscida infection[J].Fish Pathology,33:287-292;
[115].Kodama,H,Hirota,Y.Baba,T,.(1993).Activation of rainbow trout phagocytes by muramyl dipeptide[J].Dev.Comp.lmmunol,17:129-140;
[116].Laura Canesi,Caterina Ciacci,Lucia Cecilia Lorusso,et al.(2006).Immunomodulation by 17β-estradiol in bivalve hemocytes[J],inflammation and cytokines 291:664-673;
[117].M.Bagni,N.Romano,M.G.Finoia,et al.(2005).Short-and long-term effects of a dietary yeast β-glucan(Macrogard) and alginic acid(Ergosan) preparation on immune response in sea bass (Dicentrarchus labrax)[J].Fish & Shellfish Immunology 18:311-325;
[118].McKenzie,H.A.,W hite,F.H.(1991).Lsozyme and α-lactoferrin:Structure,function and interrelationships[J]..A dvanced Protein Chemistry 41,174-315;
[119].MarjaM,Antti.S.(1992),Changes in plasma lysozeme and blood leucocyte levels of hatchery-reared Atlantic Salomon and sea trout during parrsmolt transformation[J].aquaculture,106:75-87;
[122].Norma Couso,Rosario Castro,Beatriz Magarinos,et al.(2003).Effect of oral administration of glucans on the resistance of gilthead seabream to pasteurellosis[J].Aquaculture,219:99-109;
[120].Ortuno,J.,Cuesta,A.,Esteban,M.A.,et al,(2001).Efect of oral administration of high Vitamin C and E dosages on the giltheads eabream(Sparesa urataL.)innate immune system. Vet.lramunology and Immunopathology.79,167-180;
[121].Oumouna M,Jaso-Friedmann L,Evans D L.(2002).Activation of nonspecific cytotoxic cells(NCC)with synthetic oligodeoxynucleotides and bacterialgenomic DNA Binding,specificity and identification of unique immunostimulatory motifs[J].Dev Comp lmmunol,26:257-269;
[122].Peng Li,Xiaoxue Wang and Delbert M.,et al.(2006).Evaluation of levamisole as a feed additive for growth and health management of hybrid striped bass(Morone chrysops x Morone saxatilis)[J].Aquaculture 251:201-209;
[123].Poksch A,Wanger H.(1987).Structral analysis of 4-o-methyl-glu-curon-rarabinoxylan with immunostimulating activity from Echinaeea pulpurea[J].Phytochemisty,26:1989-1993;
[124].Robinson W E J.(1998).L-chicoric acid,a dicaffeoyltaric acid inhibitor of HIV integrase,improve on the in vitro anti-HIV effect of zidovudine and a protease inhibitor(AG1350)[J]Antivitral Res,.39:101-111;
[125].Rijkers,G T.(1982).Kinetics of Immoral and cellular immune reactions in fish.immunology and Immunization offish.Developmental and ComparativeImmunolgy Suppl[J].(2):93-100;
[126].Sakai.M,K ajita,Y.,Kobayashi,M.et al.(1996).Increase in heamolytic activity of serum from rainbowtrout Oncorhynchus mykiss injected with exogenous growth hormone[J].Fish shellfish Immunol,6:615-617;
[127].Sakai,M,(1999).Currentre search status offish immunostimulants[J]..Aquaculture.172,63-92
[128].Stuppner H.(1981).An immunostimulating active principle from Echinacea purpurea Moenek[J].Z Angew P hytother,2(5):166-174;
[129].Sharif,M.,Jayawardena,P.A.H.L.,et al.(2001).Immunological parameters of Javanese carp Pundus gonionotus(Bleeker) exposed to copper and challenged with Aeromonas Hydrophila[J].Fi sh&ShellfishI mmunology.11,281-291;
[130].Sahoo,P.K.& Mukherjee,S.C.(2002).The effect of dietary immunomodulation upon Edwardsiella tarda vaccination in healthy and immunocompromised Indian major carp(Labeo rohita) Fish &Shellfish Immunology.12(1)1-16;
[131].Suheyla Karata,Dtigenci,Nazli Arda,et al.(2003).Some medicinal plants as immunostimulant for fish[J].Journal of Ethnopharmacology 88:99-106;
[132].Tassakka A R,Sakai M.(2002).CpG oligodeoxynucleotidea enhance the non-specific immune responseson carp,Cyprinus carpio[J].Aquac,209:1-10;
[133].Wendy M.Sealey and Delbert M.Gatlin III.(2002).Dietary Vitamin C and Vitamin E Interact to Influence Growth and Tissue Composition of Juvenile Hybrid Striped Bass(Morone chrysopsxM.saxatilis ) but Have Limited Effects on Immune Responses[J].The Journal of Nutrition.132:748-755;
[134].Zhang Y,Liu K,Wu L J.(200 l).Advance in studies on Echinacea Moench[J].Chin Tradit Herb Drugs,32(9),852-855.
[2].尹进,彭芝配,马建中等.(2006).松黄颗粒对小鼠单核-巨噬细胞碳廓清及其抗体生成细胞的影响[J].湖南中医学院学报.26(2):10-11;
[3].林清华,(1999).免疫学实验[M].武汉大学出版社.64-67;54-56;
[4].陈昌福,罗宇良,蔡冰.(1996).实验室水温对草鱼溶菌酶活性的影响[J].中国水产科学,3(3):24-30;
[5].王军.(2001).免疫添加物对大黄鱼血液白细胞数量及吞噬功能的影响[J].海洋科学,25(9):17-19;
[6].秦启伟.(2000).饵料维生素C对青石斑鱼的非特异性免疫调节作用[J].热带海洋,19(1):58-63;
[7].孙德文,张艳秋,黄磊.(2004).鱼类免疫增强剂的研究进展[J].江西饲料,(2):19-23;
[8].江晓路,刘树青,张朝辉等.(1999).多糖对中国对虾免疫功能的影响[J].中国水产科学,6(1):66-68;
[9].秦大莲.(2006).药理学实验指导[M].414-415;
[10].王康才,唐晓清,朱光明,(2000).紫锥菊属植物的化学成分、药理及开发利用研究进展[J].中药材23(12):780-782;
[11].李玮,郭腾,陈洪亮等.(2006).紫锥菊研究进展及在动物保健上的应用展望[J].兽药与饲料添加剂,11(3):28-30;
[12].吴江涛,刘珂.(2001).紫锥菊提取物对小鼠单核巨噬细胞系统功能的影响[J].烟台大学学报(自然科学与工程版)14(2):131;
[13].张英涛,刘文芝.(2002).松果菊属3种药用植物的DNA分子鉴别研究[J].中国中医药信息杂志9(5):11-12;
[14].刘树青,江晓路,牟海津等.(1999).免疫多糖对中国对虾血清溶菌酶、磷酸酶和过氧化物酶的作用[J]_海洋与湖沼,30(3):278-283;
[15].江晓路,刘树青,张朝晖等.(1999).多糖对中国对虾免疫功能的影响[J].中国水产科学,6(1):66-68;
[16].邵健忠,项黎新.(2001).草鱼干扰素的免疫调节功能[J].动物学报,47(4):404-411;
[17].崔青曼,张耀红.(2001).中草药、多糖复合添加剂提高河蟹机体免疫力的研究[J].水利渔业,21(4):40-41;
[18].陈昌福,曾妍雄,楠田理一.(2000).接种不同种类病原菌脂多糖对翘嘴鳜细菌性败血症的免疫保护力比较[J].华中农业大学学报[J].19(4):377-379;
[19].赵红霞,詹勇,许梓荣.(2001).水产动物免疫增强剂的研究[J],饲料研究,8,1-4;
[20].江晓路,刘树青.(1999).真菌多糖对中国对虾血清及淋巴细胞免疫活性的影响[J],动物学研究,20(1):41-43;
[21].王吉桥,徐锟,(2002).对虾对营养物质的需要量[J],大连水产学院学报,17(3):196-206;
[22].王新霞,谭北平,麦康森等,(2004).β-葡聚糖在中国对虾(Penaeus chinensis)育苗中的应用[J],高技术通讯,4,71-75;
[23].谭北平,周歧存,郑石轩等,(2004).β(1,3)/(1,6)-葡聚糖葡聚糖制剂对凡纳对虾生长及免疫力的影响[J],高技术通讯,.5,73-77;
[24].汪小锋樊廷俊丛日山等,(2005).几种免疫促进剂对中国对虾血细胞数量、形态结构以及酚氧化酶产量和活性的影响[J].水产学报 29(1):66-73;
[25].陈超然,陈昌福,(2001).鱼用疫苗的研究现状[J].水利渔业,21(5):44-45;
[26].刘云,孙峰,王丹.(2004),免疫增强剂对鲫鱼非特异性免疫功能的影响[J],海洋科学,28(9):42-45;
[27].池雪林,吴德峰,(2004).浅谈中草药免疫增强剂研究进展[J].福建畜牧兽医,26(4):12-13;
[28].孙志良,陈立祥,章怀云等.(1999)α-干扰素对中国对虾皮下及造血组织坏死杆状病毒的作用[J].湖南农业大学学报,25(2):131-132;
[29].冯蓓莉,(2002),陆森泉.乳铁蛋白的抗病毒作用[J].国外医学:微生物学分册,25(1):5-6,10;
[30].孙德文,张艳秋,黄磊.(2004).鱼类免疫增强剂的研究进展[J],江西饲料,(2):19-23;
[31].赵红霞,杨建军.(2003)免疫增强剂在水产养殖的研究进展[J],江西饲料,(4):18-21;
[32].张国辉,何瑞国,张世萍等.(2007)维生素E对黄鳝繁殖性能的影响[J].水生生物学报.25(2):177-18l;
[33].张耀武,屈文俊,李文辉.(2006)β1,3-葡聚糖对锦鲤非特异性免疫功能的影响[J].淡水渔业.第36卷第4期 53-55:
[34].王宏田,徐永立,张培军.(2000).假雄牙鲆不同组织中溶菌酶比活性的研究[J].海洋科学.24(10):7-8;
[35].王远吉.(2004).枸杞多糖对鲫鱼血清溶菌酶活性的影响[J].中国饲料.24.28-31;
[36].丁磊,黄鹤忠,吴康等.(2004).镉对鲫非特异性免疫力的影响[J].农业环境科学学报.23(1):64-66;
[37].何四旺,许国焕,吴月嫦等.(2003).低聚异麦芽糖和低聚果糖对罗非鱼生长和非特异性免疫的影响[J].中国饲料.(23):14-15;
[38].刘华忠,刘定忠,赵学明.(2004).复方中草药对彭泽螂非特异性免疫功能的影响[J].淡水渔业.34(3):31-32;
[39].刘勇,贾永红.(2006).免疫增强剂对河鲫鱼白细胞吞噬和溶菌酶活性的影响[J].安徽农业科学_35(1):111-113:
[40].罗庆华.(2002).杜仲叶粉对鲤鱼免疫力的影响[J].湖南农业大学学报.28(1):51-53;
[41].王鹏,江晓路,江艳华等(2006).褐藻低聚糖对提高大菱鲆免疫机能的作用[J].海洋科学30(8):6-9;
[42].张梁,沈建忠,敖茂权等.(2006)蛭弧菌对草鱼免疫相关酶活性的影响[J].江西农业大学学报.28(6):295-299;
[43].张梁,张伟.任小丽(2007).光合细菌对草鱼生长及免疫相关酶活性的影响[J].粮食与饲料工业 (2):37-38;
[44].沈文英,阳会军,柯慧芬.(2007).β-葡聚糖对凡纳滨对虾免疫相关酶活性的影响[J].水产科学.26(7):381-383:
[45].谢玉霖,王寿昆,辛志明等,(2007).中草药在水产动物饲料添加剂上的应用[J].福建农业科学,(3):63-65;
[46].管华诗.(1999).海水养殖动物的免疫、细胞培养和病害研究[M].济南:山东科学技术出版社,23-24;
[47].姜蕾,郑曙明(2006),五倍子对鲤鱼细菌性败血症的药效实验初报[J].水产渔业科学.22(5):460-464;
[48].叶榕,张琪瑛,杨奇伟等.(1995).低浓度混苯对作业者血浆SOD的影响[J].中国工业医学杂志,8(4):206-207;
[49].宋善俊.(1991).临床医师手册[M].上海:上海科学技术出版社,185-200;
[50].林林等,(1998)有机污染提高对虾对病原菌敏感性实验[J].海洋学报.20(1):90-93;
[51].陈昌福,罗宇良,蔡冰.(1996).实验水温对草鱼溶菌酶活性的影响[J].中国水产科学,3(3):24-30;
[52].周歧存,丁炳,郑石轩等.(2004).维生素C对凡纳滨对虾生长及抗病力的影响[J].水生生物学报,6:592-598;
[53].李明,董晓慧.(2007)中草药在水产养殖中的研究进展[J].(2):饲料工业.56-58;
[54].杨严鸥,姚峰,吴继鹏,刘涛.(2005).温度对黄颡鱼血细胞数量、大小及血清生化成分的影响[J].长江大学学报.2(11):58-63;
[55].王文辉,王吉桥,程鑫等.(2006).不同剂型维生素C对生长和几种免疫指标的影响[J].中国水产科学.13(6):951-957;
[56].朱忠勇.(1997).实用医学检验学[M].北京:人民军医出版社.368-378;
[57].陈瑛,邱子健,隋淑光等.(2003).棘尾虫酸性磷酸酶的定位及诱导表达[J].动物学报,49(2):218-223;
[58].魏炜,张洪渊,石安静.(2001).育珠蚌酸性磷酸酶活力与免疫反应关系的研究[J].水生生物学报,25(4):413-415;
[59].陈国福,宋晓玲,黄健等.(2007).A3_((α))-肽聚糖对凡纳滨对虾磷酸酶及细胞内酚氧化酶活性的影响[J].海洋水产研究.28(1):50-64;
[60].樊甄姣,刘志鸿,杨爱国等.(2006).Vc对栉孔扇贝体内水解酶和抗氧化酶活性的影响[J].海洋水产研究.27(4):12-16;
[61].王学高,白冰,李西汉等.(2006)灰树花多糖对鲫鱼免疫功能的影响[J].水产科学.25(1):9-12;
[62].王秀华.(2003).肽聚糖对对虾免疫因子的作用及其在对虾养殖中的应用[D].中国海洋大学硕士学位论文18-47;
[63].王宏田,张培军.(2000).重组酵母菌对牙鲆非特异性免疫能力的影响[J].海洋与湖沼.31(6):631-633;
[64].郝林华,孙玉喜,石红旗等.(2007).牛蒂寡糖对大菱鲆生长和免疫机能的影响[J].海洋科学进展.25(2):208-2 14;
[65].马贵华,刘六英,钟青等.(2006).中华绒鳌蟹超氧化物歧化酶的初步研究[J].水利渔业.24(4):24-25;
[66].孙焕顷.(2005)鲤鱼磷酸碱性酶(AKP)的分离纯化[J].衡水学院学报,1(3):32-35;
[67].陈清西,陈素丽,石艳等.(1996).长毛对虾碱性磷酸酶的性质[J].厦门大学学报(自然科学版),35(2):257-261;
[68].陈志伟,贾秀英.(2002).氟对家蚕血液及中肠磷酸酶活性的影响[J].农业环境保护,21(2):130-133;
[69].张红梅.(2006).甘露寡糖对鲤鱼血液生化指标的影响[J].饲料研究.(1):39-41;
[70].刘红柏,卢彤岩,张春燕等.(2006).黄芪对史氏鲟抗氧化能力及免疫力的影响[J].大连水产学院学报 2(3):231-235;
[71].罗璋,姚鹃,陈昌福等.(2007).酵母免疫多糖对受免斑点叉尾鲴免疫应答的增强作用[J].淡水渔业.37(3).2-25;
[72].李友光.(1995).中国对虾疾病与免疫机制[J].海洋科学.(4):1-3;
[73].陈缓,周玫.(1991).自由基医学[M].北京:人民军医出版社,:30-31;
[74].王丽辉.(2007).中草药与益生素对断奶仔猪生长和免疫作用的影响[J].福建畜牧兽医.29(3):21-23;
[75].张琼.(2004).维生素E对幼建鲤生产性能和免疫功能的影响[D].四川农业大学硕士论文;
[76].刘清海,冯静,李健.(2000).微生物多肽对黑鲷免疫和生长的促进作用[J].海洋科学.24(5):11-13;
[77].赵远,和占龙,陈丽雄等.(2007).日粮中添加苜草素对幼猴免疫球蛋白IgG、IgA、IgM的影响[J].实验动物科学.24(3):33-35;
[78].杨严鸥,姚峰,吴继鹏等.(2005)温度对黄颡鱼血细胞数量、大小及血清生化成分的影响[J].长江大学学报.2(11):58-59;
[79].周进.(2004).免疫增强剂-A3a肽聚糖对牙鲆免疫功能及抗病力的影响[D].上海水产大学硕士论文:
[80].刘勇,杨季芳,陈昌福.(2007).免疫增强剂对河鲫鱼白细胞吞噬和溶菌酶活性的影响[J].(2):11-15;
[81].姚红梅.(2005).杜仲提取对彭泽鲫抗病和促生长研究[D].湖南农业大学硕士论文;
[82].朱晓,徐祝封,陈振武.(1997).中药理化鉴定[M].北京:中国中医药出版社;
[83].A.Cuesta,J.Meseguer and M.A.Esteban,(2004),Total serum immunoglobulin M levels are affected by immunomodulators in seabream(Sparus aurata L.)[J],Veterinary Immunology and Immunopathology101:203-210;
[84].Anderson,D.P,Siwicki,A.K.(1995).Injection or immersion delivery of selected immunostimulants to trout demonstrate enhancement of nonspecific defense mechanisms and protective immunity[J].Diseases in Asian Aquaculture,11:413-426;
[85].Anderson,D.P,(1996).Environmental factors in fish health:Immunological aspects.In the fish immune system.Organism,pathogen,and environment(Glwama&T.Nakanishi.eds)289-310.London:Academic press;
[86].Alberto Cuesta,Alejandro Rodriguez,Irene Salinas,et al.(2007).Early local and systemic innate immune responses in the teleost gilthead seabream after intraperitoneal injection of whole yeast cells[J].Fish & Shellfish Immunology.22,(3):242-251;
[87].Bany J,Siwicki AK.(2003).Echinacea purpurea stimulates cellular immunity and anti-bacterial defence independently of the strain of mice[J].Pol J Vet Sci,3(6):3-5;
[88].Berczi I.,Nagy E.(1987).The effect of prolactin and growth hormone on hemolymphopoietic tissue and immune function[M].Hormone and lmmunity,MTP Press,Lancaster,.145-147;
[89].Binns S E,Livesey J F,Arnason J T.(2002).Phytochemical variation in Echinacea from roots and flowerheads of wild and cultivated populations[J],J.Agric.Food Chem,50(13):3673-3687;
[90].Bodinet C,Mentel R.(2002).Effect of oral application of an immunomodulating plant extract on influenza virus type A infection in mice[J].Planta Med,68(10):896-900;
[91].Currier N L,Miller S C.(2000).Natural killer cells from aging mice treated with extracts from Echinacea purpurea are quantitatively and functionally rejuvenated[J].Exp Gerontol,.35(5):627-639;
[92].Chen Changfu,Li Jing,Riichi Kusuda.(1996).A preliminary study on the adjuvanticity of henbane in oral vaccination of grass carp(Ctenopharyngodon idellus)[J].华中农业大学学报,15(2):157-162;
[93].Cuesta,A.,Ortuno,J.,Podriguze,M.A.,et al,(2002).Changes in some defences par am ete rso fs eabream(Sparesa yrataL.)induced by retinol acetate.Fish&Shellfish Immunology.13,279-291;
[94].Cook,M.T,Haybill,P.J.,Hunchinson,et al,(2003).Administration of a commercial immunostimulant perparation,EcoActiva as a feed supplement enhances macrophage respiratory burst and the growth rate of snapper(Pagrus aratus,Sparidae(Bloch and Schneider))in winter.Fish&Shellfish Immunology.14,333-345;
[95].Chuntao Yuan,Dongmei Li,Wei Chen et al.(2007).Administration of a herbal immunoregulation mixture enhances some immune parameters in carp(Cyprinus carpio )[J].Fish physiology and biochemistry33.93-101;
[96].Chen S-C,Yoshida T,Adams A,et al.(1998) Non-specific immune response of Nile tilapia,Oreochronis nilotica,to the extracellular productsof Mycobacterium spp and to various adjuvants.Journal of Fish Diseases,21:39-46;D.Marionnet,P.Deschaux and S.Reynaud.(2006).Possible implication of macrophages in the regulation of cytochrome P450 activities in carp(Cyprinus carpio)[J].Fish & Shellfish Immunology,21(1):80-91;
[97].Esteban M A,Mulem V,Cuesta A et al.(2000).Effect of injecting chitin particles on the innate immune response of gilthead seabream(Spares aurata L)[J].Fish and Shellfish Immunology,10:543-554;
[98].Eduardo M.Leano,Jiin-Ju Guo et al.(2003).Levamisole Enhances Non-Specific Immune Response of Cobia,Rachycentron canadum,Fingerlings[J].J.Fish.Soc.Taiwan.30(4):321-330;
[99].Fsteban M A,Cuesta A,Ortuno J et al.(2001).Immunomodulatory effects of dietary intake of Chitin on gilthead seabream(Spares aurata L.)innate immune system[J].Fish and Shellfish Immunology,11:303-315;
[100].Freier DO,Wright K.(2003).Enhancement of the humoral immune response by Echinacea purpurea in female Swiss mice[J].lmmunopharmacol lmmunotoxicol,25(4):551-560;
[101].Guanghong Wu,ChuntaoYuan,Meifang Shen.et al.(2007).Immunological and biochemical parameters in carp(Cyprinus carpio) after Qompsell feed ingredients for long-term administration[J].38(3),246-255;
[102].Galindo-Villegas Jorge et al,(2006).Effect of Continuous and Interval Administration of Peptidoglycan on Innate Immune Response and Disease Resistance in Japanese Flounder Paralichthys olivaceus[J].Suisan Zoshoku.54;(2):163-170;
[103].Galindo-Villegas Jorge Fukada Haruhisa.Masumoto Toshiro et al.(2006 ).Effect of Dietary Immunostimulants on Some Innate Immune Responses and Disease Resistance against Edwardsiella tarda Infection in Japanese Flounder(Paralichthys olivaceus)[J].54(2):153-162;
[104].Hironobu Watanuki,Kazuki Ota,Asmi Citra Malina A.R.et al.(2006).Immunostimulant effects of dietary Spirulinaplatensis on carp,Cyprinus carpio[J].Aquaculture 258:157-163;
[105].Hou Ya Yi.(2001).Effects of estradiol-17β on immunocompetence in rainbow trout(on corhynchus mykiss)[J].Aeta Zoologica sinica 47(3):258-291;
[106].Itami T.,Kondo M.,Uozu M..et ai.(1996).Ehancement of disease resistance against Enterococcus seriolicida infection in yellowtail Seriola quinqueradiata Temminck and Schlegel by oral administration of Peptidoglycan derived from Bifidobacterim thermophilum[J].J.Fish Dis.,19:185-187;
[107].Itami Toshiaki,Masaya Asano.(1998).Enhancement of disease resistance of kuruma shrim o,penaeus japonicus after oral administration of peptidoglican derived from Bifidobacterium thermopilum[J].Aquaculture,164:277-288;
[108].J.Skjermo,Q.Bergh.(2004).High-Malginateimmunostimulation of Atlantic halibut(Hippoglossus hippoglossus L.) larvae using Artemia for delivery,increases resistance against vibriosis[J].Aquaculture,238:107-113;
[109].Jaya Kumari and P.K.Sahoo,(2006).Non-specific immune response of healthy and immunocompromised Asian catfish(Clarias batrachus) to several immunostimulants[J],Aquaculture255:133-141;
[110].John Paul.(2007).The influence of microcystin-LR on fish phagocytic cells[J].Human & Experimental Toxicology,26(7):603-607;
[111].Kajita,Y,Sakas,M,Atsuta,S.(1992).Immunopotentiantion Activety of Freund's complete adjuvant in rainbow trout[J],Oncorhynchus mykiss.Fish Shellfish lmmunol,2:155-157;
[112].Kajita Y.(1996).Erthancement of non-specific cytotoxic activity of leucocytes in rainbow trout Oncorhynchus mykiss injected with exogenous growth hormone[J].Fish&Shellfish Immunology,(6):615-617;
[123].Kakuta,I.,Kurokura,H.(1996).Defensive effect of orally administered bovine lactoferrin against Cryptoicaryon irritants infection of red seabream[J].Fish Pothlogy,30:289-290;
[114].Kawakami H.,Dosako S.(1998).The non-specific immunostimulation and adjuvant effects of Vibrio anguillarum bacterin,M-glucan,chitin or Freund's complete adjuvant in yellow seripla quinqueradiata to Pasteurella piscida infection[J].Fish Pathology,33:287-292;
[115].Kodama,H,Hirota,Y.Baba,T,.(1993).Activation of rainbow trout phagocytes by muramyl dipeptide[J].Dev.Comp.lmmunol,17:129-140;
[116].Laura Canesi,Caterina Ciacci,Lucia Cecilia Lorusso,et al.(2006).Immunomodulation by 17β-estradiol in bivalve hemocytes[J],inflammation and cytokines 291:664-673;
[117].M.Bagni,N.Romano,M.G.Finoia,et al.(2005).Short-and long-term effects of a dietary yeast β-glucan(Macrogard) and alginic acid(Ergosan) preparation on immune response in sea bass (Dicentrarchus labrax)[J].Fish & Shellfish Immunology 18:311-325;
[118].McKenzie,H.A.,W hite,F.H.(1991).Lsozyme and α-lactoferrin:Structure,function and interrelationships[J]..A dvanced Protein Chemistry 41,174-315;
[119].MarjaM,Antti.S.(1992),Changes in plasma lysozeme and blood leucocyte levels of hatchery-reared Atlantic Salomon and sea trout during parrsmolt transformation[J].aquaculture,106:75-87;
[122].Norma Couso,Rosario Castro,Beatriz Magarinos,et al.(2003).Effect of oral administration of glucans on the resistance of gilthead seabream to pasteurellosis[J].Aquaculture,219:99-109;
[120].Ortuno,J.,Cuesta,A.,Esteban,M.A.,et al,(2001).Efect of oral administration of high Vitamin C and E dosages on the giltheads eabream(Sparesa urataL.)innate immune system. Vet.lramunology and Immunopathology.79,167-180;
[121].Oumouna M,Jaso-Friedmann L,Evans D L.(2002).Activation of nonspecific cytotoxic cells(NCC)with synthetic oligodeoxynucleotides and bacterialgenomic DNA Binding,specificity and identification of unique immunostimulatory motifs[J].Dev Comp lmmunol,26:257-269;
[122].Peng Li,Xiaoxue Wang and Delbert M.,et al.(2006).Evaluation of levamisole as a feed additive for growth and health management of hybrid striped bass(Morone chrysops x Morone saxatilis)[J].Aquaculture 251:201-209;
[123].Poksch A,Wanger H.(1987).Structral analysis of 4-o-methyl-glu-curon-rarabinoxylan with immunostimulating activity from Echinaeea pulpurea[J].Phytochemisty,26:1989-1993;
[124].Robinson W E J.(1998).L-chicoric acid,a dicaffeoyltaric acid inhibitor of HIV integrase,improve on the in vitro anti-HIV effect of zidovudine and a protease inhibitor(AG1350)[J]Antivitral Res,.39:101-111;
[125].Rijkers,G T.(1982).Kinetics of Immoral and cellular immune reactions in fish.immunology and Immunization offish.Developmental and ComparativeImmunolgy Suppl[J].(2):93-100;
[126].Sakai.M,K ajita,Y.,Kobayashi,M.et al.(1996).Increase in heamolytic activity of serum from rainbowtrout Oncorhynchus mykiss injected with exogenous growth hormone[J].Fish shellfish Immunol,6:615-617;
[127].Sakai,M,(1999).Currentre search status offish immunostimulants[J]..Aquaculture.172,63-92
[128].Stuppner H.(1981).An immunostimulating active principle from Echinacea purpurea Moenek[J].Z Angew P hytother,2(5):166-174;
[129].Sharif,M.,Jayawardena,P.A.H.L.,et al.(2001).Immunological parameters of Javanese carp Pundus gonionotus(Bleeker) exposed to copper and challenged with Aeromonas Hydrophila[J].Fi sh&ShellfishI mmunology.11,281-291;
[130].Sahoo,P.K.& Mukherjee,S.C.(2002).The effect of dietary immunomodulation upon Edwardsiella tarda vaccination in healthy and immunocompromised Indian major carp(Labeo rohita) Fish &Shellfish Immunology.12(1)1-16;
[131].Suheyla Karata,Dtigenci,Nazli Arda,et al.(2003).Some medicinal plants as immunostimulant for fish[J].Journal of Ethnopharmacology 88:99-106;
[132].Tassakka A R,Sakai M.(2002).CpG oligodeoxynucleotidea enhance the non-specific immune responseson carp,Cyprinus carpio[J].Aquac,209:1-10;
[133].Wendy M.Sealey and Delbert M.Gatlin III.(2002).Dietary Vitamin C and Vitamin E Interact to Influence Growth and Tissue Composition of Juvenile Hybrid Striped Bass(Morone chrysopsxM.saxatilis ) but Have Limited Effects on Immune Responses[J].The Journal of Nutrition.132:748-755;
[134].Zhang Y,Liu K,Wu L J.(200 l).Advance in studies on Echinacea Moench[J].Chin Tradit Herb Drugs,32(9),852-855.