小麦黄矮病防治关键技术及其新型病毒抑制剂的研究
|
摘要
小麦黄矮病(Wheat yellow dwarf)是我国重要的植物病毒病之一,由麦蚜传播大麦黄矮病毒(Barley yellow dwarf virus,BYDV)引起,导致小麦严重减产。为防治小麦黄矮病,选育抗BYDV品种是最经济有效的方法,但栽培品种中抗BYDV基因贫乏,常规育种周期长、定向性差、易带入不良性状;化学防治也是控制小麦黄矮病的主要途径之一,但主要以杀虫剂控制蚜虫为主,而且麦蚜难以准确预报。
RNA干扰(RNA interference, RNAi)是将与内源性mRNA编码区序列同源的双链RNA导入细胞后,该mRNA发生特异性降解,从而导致该基因表达沉默的现象。小干扰RNA(small interfering RNA, siRNA)作为RNAi途径的重要中介,能够高效抑制病毒复制;阻断相应的病毒粘附受体的表达,切断病毒侵染途径;而且小干扰RNA介导的抗病毒效应具有高度特异性,对非同源基因的表达没有影响,已被广泛应用于哺乳动物和人细胞体系中基因功能和抗病毒研究。
为防治小麦黄矮病及制备新型病毒抑制剂,本试验进行了如下研究:
1.采用病毒抑制剂和杀虫剂相结合的方式进行了小麦黄矮病的田间药效试验。在小麦起身期,将大麦黄矮病毒GAV株系接种于陕恳9740上,喷施杀虫剂(2000倍液2.5%吡虫啉可湿性粉剂)与病毒抑制剂(500倍液20%病毒A可湿性粉剂、500倍液3.95%病毒必克可湿性粉剂、500倍液5%菌毒清水剂和300倍液2%菌克毒克水剂)组合后,于小麦开花期,统计发病率、病情指数和小麦叶片黄化率,结果表明,各组合均能早期预防和防治小麦黄矮病发生和发展,且预防效果优于防治效果,其中病毒必克与吡虫啉组合的平均预防和防治效果较显著,分别为58.08%和53.03%;
2.初步研究了病毒必克对BYDV的作用机制。利用500倍液3.95%病毒必克可湿性粉剂,在室内进行的防治性试验,选用分析纯丙酮和分析纯乙醇混合液(2:1)浸泡提取叶绿素,测量叶绿素后发现,病毒必克可有效抑制GAV对叶绿素的破坏,可减轻大麦黄矮病毒对小麦的为害。
3.初步制备了靶向BYDV-GAV株系依赖RNA的RNA聚合酶(RNA-dependent RNA polymerase, RdRp)和外壳蛋白(Coat protein, CP)的生物制剂。依据BYDV-GAV基因组全序列,设计并合成了siRNA的寡核苷酸序列,与含有转移DNA(Transferred-DNA, T-DNA)整合必需序列、卡那霉素抗性选择标记基因(nptⅡ)和35S启动子的双元表达载体pBI121连接,构建了重组表达载体pBI/siRNA,转至大肠杆菌鉴定繁殖后,再转至根癌农杆菌中,经电泳鉴定,质粒pBI/siRNA已转到根癌农杆菌中,表明得到了可能对BYDV-GAV有干扰作用的菌株。再将该菌株28℃在LB培养基上(含50μg/ml卡那霉素)过夜培养后,所得菌丝配置后,制成了可能对BYDV-GAV有干扰作用的生物制剂。
采用病毒抑制剂和杀虫剂相结合的施药方式,有望广泛应用于由昆虫介体传播的植物病毒病的防治中;利用DNA重组技术,能获得对病毒有抑制作用的改良微生物,通过液体或固体发酵,可进行规模化生产,有助于植物病毒病的防治。
Wheat yellow dwarf is induced by barley yellow dwarf viruses (BYDV) that are vectored by aphids, is one of the most serious viral diseases. Control methods against wheat yellow dwarf are mainly based on resistant or tolerant cultivars and vector control (insecticide treatments) in China, however, the former is more economical and environmentally desirable, but the resistant gene to BYDV is scarce in wheat, and conventional breeding has the limitation of long cycle, undesirable directional selection, and is easy to bring undesirable agronomic traitsa. the latter option is more effective method,but it is difficult to forecast accurately the occurrence of aphid..
RNA interference (RNAi) is a phenomenon in which double-strand RNA (dsRNA) specifically suppresses the expression of target protein by degrading the target mRNA. Small interference RNA (siRNA), an intermediated of the RNA-interference pathway, is effective in suppression of gene expression and virus infection in animal systems and human cells. Because siRNA can inhibit virus from replication efficiently, block to express of corresponding viral adhesion receptor so as to cut transmission route of virus, and siRNA-mediate resistance to virus exerted high specificity, and can’t influence on expression of non-homologous gene.
In order to attempt to control wheat yellow dwarf with agrichemicals, and develop a new plant virus inhibitors, following trails were conducted in this paper.
1. the efficacy trial was conducted in the field in the manner of combination between pesticide and plant virus inhibitors, including sowing wheat varieties Shanken 9740 on the field plot last year, inoculating strain GAV of barley yellow dwarf virus(BYDV) with Schizaphis graminum at wheat setting stage, praying agrochemicals combination between pesticide 2.5% imidacloprid wettable power at a dilution of 2000 and plant virus inhibitors (20% Virus A wettable power at a dilution of 500, 3.95% Bilken virucide wettable power at a dilution of 500, 5% Junduqing aqueous solution at a dilution of 500 and 2% Ningnanmycin aqueous solution at a dilution of 300) three times, investigating incidence and disease index of wheat yellow dwarf as well as the number of yellowing leaf at flowering stage, the results showed that all combinations have preventive and control effect on accidence and development of wheat yellow dwarf, and the preventive effect is superior to the control effect. Among the above combinations, the combination of Bilken viruaside and imidacloprid was most significant, and the average preventive and control effect were 58.08% and 53.03%,respectively.
2. The influence of Bilken viruaside on inhibition of BYDV from the harm of chlorophyll was researched under Indoor condition. Chlorophyll in wheat leaves,extracted by mixed soak with analytically pure acetone and analytically pure ethanol(2:1),can be measured in test which wheat yellow dwarf was controlled by 3.95% Bilken virucide wettable power at a dilution of 500 in laboratory, the result indicated that Bilken viruaside can inhibit BYDV from the harm of chlorophyll so as to relieve the damage.
3. A new plant virus inhibitors contained small interfering RNA aimed to RNA-dependent RNA polymerase and coat protein of BYDV-GAV was prepared on laboratory platform. In order to prove interference of the homologous siRNA with BYDV-GAV, oligonucleotides expressed siRNA were designed and synthesized according to the target protein gene of RNA-dependent RNA polymerase and coat protein of BYDV-GAV and sub cloned to the binary vector pBI121 which T-DNA contained the Gus reporter gene controlled by 35S promoter and nptII selectable marker gene controlled by the nopaline synthase (NOS) promoter, Then, these construction of expression vector pBI121/siRNA were introduced into A.tumefaciens strain EHA105 by freeze-thaw method. After electrophoretic analysis, vector pBI121/siRNA was confirmed to introduce into A.tumefaciens, Then,A.tumefaciens strain introduced expression vector pBI121/siRNA was incubated into LB medium contained 50μg/ml kanamycin, after overnight culture at 28℃,mycelium were developed into inhibitor by adding adjuvant.
In the future, the control method in the manner of combination between pesticide and plant virus inhibitors will be applied extensively to plant virus disease transmitted by vector. Antagonistic microorganisms can be gained by genetic engineering, and suit to the large scale production by liquid fermentation so as to contribute to control of the plant virus disease.
RNA干扰(RNA interference, RNAi)是将与内源性mRNA编码区序列同源的双链RNA导入细胞后,该mRNA发生特异性降解,从而导致该基因表达沉默的现象。小干扰RNA(small interfering RNA, siRNA)作为RNAi途径的重要中介,能够高效抑制病毒复制;阻断相应的病毒粘附受体的表达,切断病毒侵染途径;而且小干扰RNA介导的抗病毒效应具有高度特异性,对非同源基因的表达没有影响,已被广泛应用于哺乳动物和人细胞体系中基因功能和抗病毒研究。
为防治小麦黄矮病及制备新型病毒抑制剂,本试验进行了如下研究:
1.采用病毒抑制剂和杀虫剂相结合的方式进行了小麦黄矮病的田间药效试验。在小麦起身期,将大麦黄矮病毒GAV株系接种于陕恳9740上,喷施杀虫剂(2000倍液2.5%吡虫啉可湿性粉剂)与病毒抑制剂(500倍液20%病毒A可湿性粉剂、500倍液3.95%病毒必克可湿性粉剂、500倍液5%菌毒清水剂和300倍液2%菌克毒克水剂)组合后,于小麦开花期,统计发病率、病情指数和小麦叶片黄化率,结果表明,各组合均能早期预防和防治小麦黄矮病发生和发展,且预防效果优于防治效果,其中病毒必克与吡虫啉组合的平均预防和防治效果较显著,分别为58.08%和53.03%;
2.初步研究了病毒必克对BYDV的作用机制。利用500倍液3.95%病毒必克可湿性粉剂,在室内进行的防治性试验,选用分析纯丙酮和分析纯乙醇混合液(2:1)浸泡提取叶绿素,测量叶绿素后发现,病毒必克可有效抑制GAV对叶绿素的破坏,可减轻大麦黄矮病毒对小麦的为害。
3.初步制备了靶向BYDV-GAV株系依赖RNA的RNA聚合酶(RNA-dependent RNA polymerase, RdRp)和外壳蛋白(Coat protein, CP)的生物制剂。依据BYDV-GAV基因组全序列,设计并合成了siRNA的寡核苷酸序列,与含有转移DNA(Transferred-DNA, T-DNA)整合必需序列、卡那霉素抗性选择标记基因(nptⅡ)和35S启动子的双元表达载体pBI121连接,构建了重组表达载体pBI/siRNA,转至大肠杆菌鉴定繁殖后,再转至根癌农杆菌中,经电泳鉴定,质粒pBI/siRNA已转到根癌农杆菌中,表明得到了可能对BYDV-GAV有干扰作用的菌株。再将该菌株28℃在LB培养基上(含50μg/ml卡那霉素)过夜培养后,所得菌丝配置后,制成了可能对BYDV-GAV有干扰作用的生物制剂。
采用病毒抑制剂和杀虫剂相结合的施药方式,有望广泛应用于由昆虫介体传播的植物病毒病的防治中;利用DNA重组技术,能获得对病毒有抑制作用的改良微生物,通过液体或固体发酵,可进行规模化生产,有助于植物病毒病的防治。
Wheat yellow dwarf is induced by barley yellow dwarf viruses (BYDV) that are vectored by aphids, is one of the most serious viral diseases. Control methods against wheat yellow dwarf are mainly based on resistant or tolerant cultivars and vector control (insecticide treatments) in China, however, the former is more economical and environmentally desirable, but the resistant gene to BYDV is scarce in wheat, and conventional breeding has the limitation of long cycle, undesirable directional selection, and is easy to bring undesirable agronomic traitsa. the latter option is more effective method,but it is difficult to forecast accurately the occurrence of aphid..
RNA interference (RNAi) is a phenomenon in which double-strand RNA (dsRNA) specifically suppresses the expression of target protein by degrading the target mRNA. Small interference RNA (siRNA), an intermediated of the RNA-interference pathway, is effective in suppression of gene expression and virus infection in animal systems and human cells. Because siRNA can inhibit virus from replication efficiently, block to express of corresponding viral adhesion receptor so as to cut transmission route of virus, and siRNA-mediate resistance to virus exerted high specificity, and can’t influence on expression of non-homologous gene.
In order to attempt to control wheat yellow dwarf with agrichemicals, and develop a new plant virus inhibitors, following trails were conducted in this paper.
1. the efficacy trial was conducted in the field in the manner of combination between pesticide and plant virus inhibitors, including sowing wheat varieties Shanken 9740 on the field plot last year, inoculating strain GAV of barley yellow dwarf virus(BYDV) with Schizaphis graminum at wheat setting stage, praying agrochemicals combination between pesticide 2.5% imidacloprid wettable power at a dilution of 2000 and plant virus inhibitors (20% Virus A wettable power at a dilution of 500, 3.95% Bilken virucide wettable power at a dilution of 500, 5% Junduqing aqueous solution at a dilution of 500 and 2% Ningnanmycin aqueous solution at a dilution of 300) three times, investigating incidence and disease index of wheat yellow dwarf as well as the number of yellowing leaf at flowering stage, the results showed that all combinations have preventive and control effect on accidence and development of wheat yellow dwarf, and the preventive effect is superior to the control effect. Among the above combinations, the combination of Bilken viruaside and imidacloprid was most significant, and the average preventive and control effect were 58.08% and 53.03%,respectively.
2. The influence of Bilken viruaside on inhibition of BYDV from the harm of chlorophyll was researched under Indoor condition. Chlorophyll in wheat leaves,extracted by mixed soak with analytically pure acetone and analytically pure ethanol(2:1),can be measured in test which wheat yellow dwarf was controlled by 3.95% Bilken virucide wettable power at a dilution of 500 in laboratory, the result indicated that Bilken viruaside can inhibit BYDV from the harm of chlorophyll so as to relieve the damage.
3. A new plant virus inhibitors contained small interfering RNA aimed to RNA-dependent RNA polymerase and coat protein of BYDV-GAV was prepared on laboratory platform. In order to prove interference of the homologous siRNA with BYDV-GAV, oligonucleotides expressed siRNA were designed and synthesized according to the target protein gene of RNA-dependent RNA polymerase and coat protein of BYDV-GAV and sub cloned to the binary vector pBI121 which T-DNA contained the Gus reporter gene controlled by 35S promoter and nptII selectable marker gene controlled by the nopaline synthase (NOS) promoter, Then, these construction of expression vector pBI121/siRNA were introduced into A.tumefaciens strain EHA105 by freeze-thaw method. After electrophoretic analysis, vector pBI121/siRNA was confirmed to introduce into A.tumefaciens, Then,A.tumefaciens strain introduced expression vector pBI121/siRNA was incubated into LB medium contained 50μg/ml kanamycin, after overnight culture at 28℃,mycelium were developed into inhibitor by adding adjuvant.
In the future, the control method in the manner of combination between pesticide and plant virus inhibitors will be applied extensively to plant virus disease transmitted by vector. Antagonistic microorganisms can be gained by genetic engineering, and suit to the large scale production by liquid fermentation so as to contribute to control of the plant virus disease.
引文
[1] Oswald JW, Houston BR. A new virus disease of cereals, transmissible by aphids [J]. Plant Disease Reports, 1951, 35: 471-475.
[2] Watson MA, Mulligan TE. Cereal yellow dwarf virus in Great Britain [J]. Plant Pathol, 1957, 6: 12-14.
[3] Pocsai E, Murányi I, Papp M, et al. Incidence of barley yellow dwarf viruses in symptom-exhibiting cereal species [J]. Proceedings of an International Symposium, 2002, El Batán, Texcoco, Mexico.
[4] Smith HC. Notes on new diseases in New Zealand [J]. Commonw. Phytopathol. News, 1955, 1: 10-11.
[5] Smith HC. Cereal yellow dwarf virus seen in Australia [J]. Commonw. Phytopathol. News, 1957, 3: 10-11.
[6] Miller WA, Rasochova L. Barley yellow dwarf viruses [J]. Annu. Rev. Phytopathol, 1997, 35: 167-190.
[7] Borgemeister C, Doehling HM. Reasons for and consequences of the serious BYDV infestation in Northern Germany [J]. Bulletin in SROP (1991), 1989, 14(4): 60-66.
[8] Gill CC. Assessment of losses on spring wheat naturally infected with barley yellow dwarf virus [J]. Plant Disease, 1980, 64: 197-203.
[9] Pocsai E, Kobza S. Epidemiological occurrence of barley yellow dwarf virus in Hungary. Proceedings of the International Conference of Integrated Plant Protection, 1983.
[10]刘艳. BYDV-GPV与GAV混合侵染蚜传专化性研究[D].北京:中国农业科学院, 2004.
[11]范绍强,谢咸升,李峰等.冬小麦黄矮病预测模型研究[J].中国生态农业学报, 2006, 14(2): 147-149.
[12]赵多长.天水市1999年小麦黄矮病流行原因分析与综防对策[J].甘肃农业科技, 2000 (4): 38-39.
[13]周广和,张淑香,罗乔WF.一种由麦二叉蚜和麦长管蚜传播的小麦黄矮病毒株系的鉴定[J].植物保护学报, 1986, 16(1): 17-21.
[14]范绍强,郑王义,谢咸生等.山西小麦黄矮病流行趋势预测模型探讨[J].农业网络信息, 2006(05) :176-177
[15]周广和,张淑香,钱幼亭.小麦黄矮病毒4种株系鉴定与应用[J].中国农业科学,1987,20(4):7-12.
[16]燕飞. hpRNA和pac1基因介导抗大麦黄矮病毒转基因小麦研究[D].北京:中国农业科学院,2006.
[17]赵彦宏,李润植,刘征.大麦BYDV抗性的印迹杂交分析[J].作物学报, 2003, 29(2):315-320.
[18]钱幼亭,周广和.小麦黄矮病寄主范围的测定[J].植物保护, 1987(1): 20-21.
[19]吴云峰,李毅,魏宁生等.大麦黄矮病毒(BYDV-GPV )的循回传播机理[J].西北农业大学学报, 1997, 25(6): 11-14.
[20] Gray SM, Rochon DA. Vector transmission of plant viruses [M]. In: A.Granoff and R.G Webster (eds) Encyclopedia of Virology, 1999, 2nd edn, pp.1899-1910. Academic Press, San Diego.
[21] Gilow FE. Luterovirus transmission and mechanisms regulating vector specificity. In:. Smith HG, Baker H (eds) . The Luteroviridas, 1999, pp. 88-112. CAB International, Wallingford, UK.
[22]李经略,赵玉霞,赵君东.小麦黄矮病(BYDV)寄主范围的研究[J].中国农业科学, 1985, 18 (06): 7-13.
[23] Hsu HT, Aebig J, Rochow WF. Difference among monoclonal antibody to barley yellow dwarf viruses [J]. Physiopathology, 1984, 74: 600-605.
[24]张秦风,朱象三,关蕴瑞等.小麦黄矮病毒株系鉴定结果简报[J].陕西农业科学, 1981 (06): 13-14.
[25]李长全,刘振汉.小麦黄矮病综合防治措施[J].青海农技推广, 2005 (3): 56-58.
[26]张文斌,安德荣,任向辉.中国小麦黄矮病的发生与综合防控研究进展[J].麦类作物学报, 2009, 29(2): 361-364.
[27]王晓萍.抗黄矮病小麦易位系基因组TAC和cDNA文库的构建及筛选的研究[D].北京:中国农业科学院, 2002.
[28]谢皓,陈孝.我国抗黄矮病小麦研究进展[J].北京农学院学, 1999, 14(2): 55-57.
[29] Fabre F, Kervarrec C, Mieuzet L, et al. Improvement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real time RT-PCR [J]. Journal of Virological Methods, 2003, 110: 51-60.
[30] Chain F, Riault G, Trottet M, et al. Analysis of accumulation patterns of barley yellow dwarf virus-AV (BYDV-PAV) in two resistant wheat lines [J]. European Journal of Plant Pathology, 2005, 113: 343–355.
[31]王孑民,张建民,赵子俊等.香茅草二叉蚜不是小麦黄矮病的传毒介体[J].山西农业科学, 1984(9): 15-16.
[32]刘艳,王锡锋,周广和等.大麦黄矮病毒的特异性引物、探针制备及快速检测方法[P].中国, 200510011977.4. 2005-12-21.
[33]相建业,冯崇川,马曙.麦蚜发生规律及预报研究[J].西北农业学报, 1994, 3(1): 90-94.
[34]冯崇川.冬小麦黄矮病春季流行指标与预测预报的研究[J].植物保护学报, 1983, (2): 118-121.
[35]相建业,冯崇川.小麦黄矮病预测预报[J].植物保护学报, 1994, 21(1): 73-77.
[36]钱幼亭,周广和.麦类种质资源抗耐小麦黄矮病毒的田间鉴定技术研究[J].植物保护学报, 1986(03): 159-164.
[37]苗洪芹,陈巽桢,吴和平.承德春小麦黄矮病株系鉴定[J].河北农业大学学报, 1996, (19)3: 29-33.
[38]宋庆杰.黑龙江省小麦抗黄矮病研究初报[J].麦类作物, 1999, 19(3): 32-34.
[39]刘艳,钱幼亭,赵茂林等.多枝赖草及其转育后代对大麦黄矮病毒PAV和GAV株系的抗性研究[J].植物病理学报, 2002, 32(3): 247-251.
[40]刘小东,张增艳,刘艳等.抗病基因Bdv2抑制大麦黄矮病毒复制和运动的分子证据[J].遗传学报, 2005, 32(9): 942-947.
[41]张秦风,任芝英,金欣藻.关于小麦品种抗黄矮病毒的鉴定问题[J].植物保护, 1984, (6): 13-14.
[42]范绍强,谢咸升,郑王义等.小麦黄矮病遗传育种研究进展[J].中国生态农业学报, 2008, 16(1): 241-244.
[43]张增艳,辛志勇,陈孝等.源于L1的小麦抗黄矮病基因的特异PCR标记及辅助育种的研究[J].作物学报, 2002, 28(4): 486-491.
[44]马有志,徐琼芳,辛志勇等.抗黄矮病小麦种质的分子标记[J].作物学报, 1999, 25(4):433-436.
[45]武东亮.偃麦草(Agropyron pulcherrimum )黄矮病抗性向小麦的导入与鉴定[D].北京:中国农科院研究生院, 1997.
[46]李永丽,史洪中,陈利军等.转基因小麦抗大麦黄矮病毒研究进展[J].安徽农业科学, 2006, 34(21): 5451-5453.
[47]张增艳,辛志勇.抗黄矮病小麦生物技术育种研究进展[J].作物杂志,2005(5):4-7.
[48]王顺建,刘光荣,王向阳等.麦蚜天敌利用技术的研究[J].植保技术与利用,2002,22(10): 10-12.
[49]王随保.麦类病毒病及其防治[J].山西农业科学, 1984(9): 44-47.
[50]曹庆.河西种冬麦不是黄矮病大发生的唯一原因[J].甘肃农业科技, 1982(01):6-8.
[51]李晋梅. 1999年晋南小麦黄矮病流行原因及防治对策[J].农业科技通讯, 1999(10): 31.
[52]许仁林. PR-蛋白与高等植物的诱导抗性[J].病毒学杂志, 1989, (2): 121-123.
[53]江山,韩熹莱.植物病毒病化学防治的研究进展[J].中国病毒学, 1995, 10(l): 1-7.
[54]李在国,黄润秋,杨华铮等.化学合成植物病毒抑制剂研究进展[J].农药, 1998, 37(5): 3-6.
[55]安德荣.植物病毒化学防治的研究现状和所面临的问题[J].生命科学, 1994, 6(2): 15-18.
[56] Hohne C, Schuster G, Frommhold I. Growth Regulatory Effects of 5-Azadihydrouracil and Ribavirin [J]. Biochemistry Physiology Pflanzen, 1987, 182: 375-384.
[57]许良忠,郭玉晶,张书圣.植物病毒病化学防治的研究进展[J].青岛化工学院学报, 2000, 21(4): 293-297.
[58]许良忠,郭玉晶,张书圣.植物病毒防治剂盐酸吗啉呱的合成研究[J].沈阳化工学院学报, 2001, 15(2): 89-91.
[59]张华,宋宝安.杂环类植物抗病毒剂的研究进展[J].农药, 2002, 41(2): 6-9.
[60]陈齐斌,沈嘉样.抗植物病毒剂研究进展和面临的挑战与机遇[J].云南农业大学学报, 2005, 20(4): 505-512.
[61]赵玖华,尚佑芬,王升吉等.植物源抗病毒活性物质研究进展[J].石河子大学学报(自然科学版), 2004, 22(增刊).
[62]朱水方,裘维蕃.几种中草药抽提物对黄瓜花叶病毒引起的辣椒花叶病治疗作用初步研究[J].植物病理学报, 1989, 19(2): 123-128.
[63] Duggar BM, Armstrong JK. The effect of treating the virus of tobacco mosaic with the juice of various plants [J]. Annals of the Missouri Botanical Garden, 1925, (12): 359-366.
[64] Chen ZC, Antoniw JF, Lin Q, et al. A possible mechanism for the antiviral activity of pokeweed antiviral protein [J]. Physiol Mol Plant Pathol. 1993, 42(4): 249.
[65] Wyatt SD, Shepherd RJ. Isolation and characterization of a virus inhibitor from Phytolacca Americana [J]. Phytopathology, 1969, 59: 1987.
[66] Kuntz JE, Walker SC. Virus Inhibitor by Extracts of Spinach [J]. Phytopathology, 1947, 37: 561-579.
[67] Gianinazzi S, Kassanis B. Virus resistance induced in plants by polyacrylic acid [J]. J Gen Virology, 1974, 23: 1-9.
[68]林存銮,裘维蕃.一些植物抽提液对番茄花叶病毒病(TMV-T)的治疗作用[J].植物保护学报, 1987, 14(4): 217-219.
[69] Tsutomu I, Youichi T, Seiko I, et al. Formation of anti-plant viral protein by Mirabilis jalapa L. cells in suspension culture [J]. Plant Cell Reports, 1987, 6(3): 216-218.
[70] Susumu K, Tsutomu I, Seiko I, et al. A potent Plant virus inhibitor found in Mirabilis jalapa L. [J]. Annal Phytopathology Society of Japan, 1990, 56: 481-487.
[71] Verma HN, Awasthi LP. Isolation of the virus inhibitor from the root extract of Boerhaavia diffusa inducing systemic resistance in plants [J]. Canada Journal Botany, 1979, 57: 1214-1217.
[72]刘道贵,程伟星,汤牛根等.植物抽提物防治植物病毒病的研究[J].安徽农业科学, 2000, 28(6): 764-766.
[73]原征彦,冈田文雄等. [P].日本特开平6-199619, 1994.
[74] Chen ZC, White JF. Effect of pokeweed antiviral protein (PAP) on the infection of plant viruses [J]. Plant Pathology, 1991, 40: 612-620.
[75]吴云峰,曹让.几种药剂对病毒侵染和植物抗病性的影响[J].西北农业大学学报, 1999, 27(2): 1-5.
[76]许良忠,郭玉晶.植物病毒病化学防治研究进展[J].青岛化工学院学报(自然科学版), 2000, 21(4): 293-297.
[77]史清亮.施用PSB菌剂对番茄生长发育的影响及其防病(CMV)效果[J].山西农业科学, 1994, 22(增刊): 24-26.
[78]前田浩明.关于植物病毒抑制剂Retemin[J].今月农业, 1982, 26(6): 42-47.
[79]王贵,崔卫东. 5种抗病毒剂对烟草花叶病防效的研究[J].烟草科技, 1997, (5): 45.
[80]向固西.一种新的农用抗生素—宁南霉素[J].微生物学报, 1995, 35(5): 368-374.
[81]吴云峰.生物病毒农药筛选及应用[J].世界农业, 1995, 5: 35-36.
[82]李重九,侯玉霞.植物病毒病选择性治疗药剂的筛选研究[J].植物病理学报, 1997,27(4): 343-348.
[83]江山,郭雪柳,韩熹莱.一些抗植物病毒剂对烟草花叶病毒衣完蛋白体外聚合过程的影响[J].中国病毒学, 1996, 11(1): 77-79.
[84]杜春梅,吴元华,赵秀香等.天然抗植物病毒物质的研究进展[J].中国烟草学报, 2004, 10(1): 34-39.
[85] Schuster G, Horigklee W, Winter H, et al. Dioxoherxa-hydrotriazine, eneneuevoll synthetische antiphtoviral verbndung [J]. Zentralbl Bakteriol Mikrobiol Hyg, 1979, 1334: 64-69.
[86]黄荣茂.植物速丰剂对烟草普通花叶病(TMV)的防治研究[A].中国植物病理学会第三届代表大会暨学术年会论文集[C].北京: 1997.
[87] Goswam BB, Borke E, Sharma OK, et al. The broad spectrum antiviral agent ribabivirin inhibits capping of mRNA [J]. Biochemistry and Biophysics Research Community, 1979(89): 830-835.
[88]李重九,费著.丙酰紫草素对病毒的钝化作用[J].中国农业大学学报, 1997, 2(5): 47-50.
[89] French CJ, Elder M, Leggett F, et al. Flavonoids inhibit infectivity of tobacco mosaic virus [J]. Canadian Journal of Plant Pathology, 1991, 13: 1-8.
[90]张建新,吴云锋,樊兵.多羟基双萘醛提取物WCT抗病毒的生理病理学研究[J].植物病理学报, 2005, 35(6): 514-519.
[91]杨合同,王加宁.植物抗病反应中的蛋白磷酸化[J].山东科学, 2001, 14(2): 28-33.
[92]陈启建,刘国坤,吴祖建等.大蒜挥发油抗烟草花叶病毒机理[J].福建农业学报, 2006, 21(1): 24-27.
[93]沈建国.两种药用植物对植物病毒及三种介体昆虫的生物活性[D].福州:福建农林大学博士论文, 2005.
[94]侯玉霞,李重九,马立新.中草药中抗植物病毒TMV活性物质PZ作用机理研究[J].中国农业大学学报, 2000, 5(1): 21-24.
[95]龚国淑,张庆.梨树感染茎沟病毒后生长速度与内源激素的变化[J].西南农业学报, 2002, 15(2): 62-64.
[96]吴尔福.植物病毒及其防治[M].北京:中国科学技术出版社, 1996.
[97]费著,蔡祝南,刘仪等.抗病毒剂对烟草花叶病毒与烟草叶绿体互作的影响[J].植物保护, 1998 (2): 10-13.
[98]李在国,黄润秋.植物病毒防治剂的作用机制[J].植物保护, 1999, 25(3): 37-38.
[99]雷新云,李怀芳,裘维蕃.植物诱导抗性对病毒侵染的作用及诱导物质NS-83机制探讨[J].中国农业科学, 1987, 20(4): 1-51.
[100]雷新云,李怀芳,裘维蕃. 83增抗剂防治烟草病毒病研究进展[J].北京农业大学学报, 1990, 16(3): 241-248.
[101]刘学端,肖启明.植物源农药防治烟草花叶病机理初探[J].中国生物防治, 1997,13(3): 128-131.
[102]孙凤成,雷新云.耐病毒诱导剂88-D诱导珊西烟产生PR蛋白及对TMV侵染的抗性[J].植物病理学报, 1995, 25(4): 345-349.
[103]李兴红,贾月梅,商振清等. VA系统诱导烟草对TMV抗性与细胞内防御酶系统的关系[J].河北农业大学学报, 2003, 26(4): 21-24.
[104]刘联仁.烟草病虫防治[M].北京:科学出版社, 1998.
[105] Fodor J, Gullner G, Adam AL, et al. Local and systemic responses of antioxidants to tobacco mosaic virus infection and to Salicylicacid in tobacco (role in systemic acquired resistance) [J]. Plant Physiology, 1997(114): 1443-1451.
[106]殷培军,马志卿,冯俊涛等. VFB诱导烟草叶片生理生化变化与TMV抗性的关系[J].西北农业学报, 2005, 14(2): 91-95.
[107]吴尔福,张举仁,孙光荣. TA(TS剂)制剂防治玉米、粟红叶病的生理效应[J].上海农业学报, 1989, 5(1): 67-72.
[108]朱学文,李凤梅. RNA干扰及其在植物中的应用进展[J].长江大学学报(自科版)农学卷,2007, 4(3): 96-99.
[109]吴大利,侯岁稳.干扰RNA( RNAi)作用机理及其在植物细胞工程中的应用进展[J].中国生物工程杂志, 2006, 26(9) :84-90.
[110]赵明敏,赵健,郭永清等. RNA干扰及其在植物研究中的应用[J].农业生物技术科学,2006, 22 (4): 36-39.
[111]毛颖波,薛学义,陈晓亚.植物小RNA与RNA干扰:生物学功能与应用前景[J].2009,39(1): 31-43.
[112]燕飞,成卓敏. RNA干扰技术在几项研究领域的应用[J].中国生物工程杂志, 2004, 24 (8):14-19.
[113]王志坤,李文滨,刘珊珊. RNA干扰技术及在植物抗病研究中的应用[J].大豆科学, 2008, 27 (3):521-526.
[114] Abel PP , Nelson RS, De B, et al. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene [J]. Science, 1986, 232(4751): 738-742.
[115] Ma ZL, Yang HY, Wang R, et al. Construct hairpin RNA to fight against rice dwarf virus [J]. Acta Botanica Sinica, 2004, 46(3): 332-336.
[116] Wang MB, Abbott DC, Waterhouse PM. A single copy of a virus-derived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus [J].Mol. Plant Pathol, 2000, 1(6): 347-356.
[117]燕飞,张文蔚,肖红等.转病毒来源发夹RNA小麦表现对大麦黄矮病毒的抗性[J].遗传,2007,29(1): 97-102.
[118] Tenllado F, Llave C, Diaz-Ruiz JR. RNA interference as a new biotechnological tool for the control of virus diseases in plants [J]. Virus Research, 2004, 102: 85-96.
[119] Kalantidis K, Psaradakis S, Tabler M, et al. The occurrence of CMV-specific short RNAs in transgenic tobacco expressing virus-derived double-stranded RNA is indicative of resistance to the virus [J]. MPM I, 2002, 15(8): 826-833.
[120] Takahashi S, Komatsu K, Kagiwada S, et al.The efficiency of interference of Potato virus X infection depends on the target gene [J]. Virus Research, 2006( 116): 214-217.
[121] Ramachandran V, Padmanabhan C, Claude MF. Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells [J]. Proc Natl Acad Sci USA. 2002, 100(16), 9632–9636.
[122]牛颜冰,于翠,张凯等.瞬时表达黄瓜花叶病毒部分复制酶基因和番茄花叶病毒移动蛋白基因的dsRNA能阻止相关病毒的侵染[J].农业生物技术学报, 2004, 12(4):484-485.
[123]赵明敏,安德荣,黄广华等.瞬时表达靶向TMV外壳蛋白的siRNA能干扰病毒侵染[J].植物病理学报,2006, 36(1):35-40.
[124] Zhao MM, Yang XD, Lu HX, et al. siRNA-directed Interference of TMV RNA Accumulation in Tobacco Protoplast [J]. Acta Phytopathologica Sinica, 2006, 37(3): 284-288.
[125] Zhao MM, An DR, Huang GH, et al. A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection. Acta Biochim Biophys Sin (Shanghai). 2005, 37(4): 248-253.
[126]彭运生.关于提取叶绿素方法的比较研究[J].北京农业大学学报, 1992, 18 (3): 247- 250
[127] R.赫尔(编著),范在丰,李怀方等(译).马修斯植物病毒学[M].北京:科学出版社, 2007, 763-764.
[128]景永学.植病灵防治小麦黄矮病效果初报[J].甘肃农业科技, 1995, 5:39.
[129]杨洪元,廖旭辉.植物病毒病防治策略的研究进展[J].安徽农学通报, 2006, 12 (3): 74-76.
[130]赵明敏. siRNA干扰TMV侵染及其分子机理研究[D].杨凌:西北农林科技大学, 2005.
[2] Watson MA, Mulligan TE. Cereal yellow dwarf virus in Great Britain [J]. Plant Pathol, 1957, 6: 12-14.
[3] Pocsai E, Murányi I, Papp M, et al. Incidence of barley yellow dwarf viruses in symptom-exhibiting cereal species [J]. Proceedings of an International Symposium, 2002, El Batán, Texcoco, Mexico.
[4] Smith HC. Notes on new diseases in New Zealand [J]. Commonw. Phytopathol. News, 1955, 1: 10-11.
[5] Smith HC. Cereal yellow dwarf virus seen in Australia [J]. Commonw. Phytopathol. News, 1957, 3: 10-11.
[6] Miller WA, Rasochova L. Barley yellow dwarf viruses [J]. Annu. Rev. Phytopathol, 1997, 35: 167-190.
[7] Borgemeister C, Doehling HM. Reasons for and consequences of the serious BYDV infestation in Northern Germany [J]. Bulletin in SROP (1991), 1989, 14(4): 60-66.
[8] Gill CC. Assessment of losses on spring wheat naturally infected with barley yellow dwarf virus [J]. Plant Disease, 1980, 64: 197-203.
[9] Pocsai E, Kobza S. Epidemiological occurrence of barley yellow dwarf virus in Hungary. Proceedings of the International Conference of Integrated Plant Protection, 1983.
[10]刘艳. BYDV-GPV与GAV混合侵染蚜传专化性研究[D].北京:中国农业科学院, 2004.
[11]范绍强,谢咸升,李峰等.冬小麦黄矮病预测模型研究[J].中国生态农业学报, 2006, 14(2): 147-149.
[12]赵多长.天水市1999年小麦黄矮病流行原因分析与综防对策[J].甘肃农业科技, 2000 (4): 38-39.
[13]周广和,张淑香,罗乔WF.一种由麦二叉蚜和麦长管蚜传播的小麦黄矮病毒株系的鉴定[J].植物保护学报, 1986, 16(1): 17-21.
[14]范绍强,郑王义,谢咸生等.山西小麦黄矮病流行趋势预测模型探讨[J].农业网络信息, 2006(05) :176-177
[15]周广和,张淑香,钱幼亭.小麦黄矮病毒4种株系鉴定与应用[J].中国农业科学,1987,20(4):7-12.
[16]燕飞. hpRNA和pac1基因介导抗大麦黄矮病毒转基因小麦研究[D].北京:中国农业科学院,2006.
[17]赵彦宏,李润植,刘征.大麦BYDV抗性的印迹杂交分析[J].作物学报, 2003, 29(2):315-320.
[18]钱幼亭,周广和.小麦黄矮病寄主范围的测定[J].植物保护, 1987(1): 20-21.
[19]吴云峰,李毅,魏宁生等.大麦黄矮病毒(BYDV-GPV )的循回传播机理[J].西北农业大学学报, 1997, 25(6): 11-14.
[20] Gray SM, Rochon DA. Vector transmission of plant viruses [M]. In: A.Granoff and R.G Webster (eds) Encyclopedia of Virology, 1999, 2nd edn, pp.1899-1910. Academic Press, San Diego.
[21] Gilow FE. Luterovirus transmission and mechanisms regulating vector specificity. In:. Smith HG, Baker H (eds) . The Luteroviridas, 1999, pp. 88-112. CAB International, Wallingford, UK.
[22]李经略,赵玉霞,赵君东.小麦黄矮病(BYDV)寄主范围的研究[J].中国农业科学, 1985, 18 (06): 7-13.
[23] Hsu HT, Aebig J, Rochow WF. Difference among monoclonal antibody to barley yellow dwarf viruses [J]. Physiopathology, 1984, 74: 600-605.
[24]张秦风,朱象三,关蕴瑞等.小麦黄矮病毒株系鉴定结果简报[J].陕西农业科学, 1981 (06): 13-14.
[25]李长全,刘振汉.小麦黄矮病综合防治措施[J].青海农技推广, 2005 (3): 56-58.
[26]张文斌,安德荣,任向辉.中国小麦黄矮病的发生与综合防控研究进展[J].麦类作物学报, 2009, 29(2): 361-364.
[27]王晓萍.抗黄矮病小麦易位系基因组TAC和cDNA文库的构建及筛选的研究[D].北京:中国农业科学院, 2002.
[28]谢皓,陈孝.我国抗黄矮病小麦研究进展[J].北京农学院学, 1999, 14(2): 55-57.
[29] Fabre F, Kervarrec C, Mieuzet L, et al. Improvement of Barley yellow dwarf virus-PAV detection in single aphids using a fluorescent real time RT-PCR [J]. Journal of Virological Methods, 2003, 110: 51-60.
[30] Chain F, Riault G, Trottet M, et al. Analysis of accumulation patterns of barley yellow dwarf virus-AV (BYDV-PAV) in two resistant wheat lines [J]. European Journal of Plant Pathology, 2005, 113: 343–355.
[31]王孑民,张建民,赵子俊等.香茅草二叉蚜不是小麦黄矮病的传毒介体[J].山西农业科学, 1984(9): 15-16.
[32]刘艳,王锡锋,周广和等.大麦黄矮病毒的特异性引物、探针制备及快速检测方法[P].中国, 200510011977.4. 2005-12-21.
[33]相建业,冯崇川,马曙.麦蚜发生规律及预报研究[J].西北农业学报, 1994, 3(1): 90-94.
[34]冯崇川.冬小麦黄矮病春季流行指标与预测预报的研究[J].植物保护学报, 1983, (2): 118-121.
[35]相建业,冯崇川.小麦黄矮病预测预报[J].植物保护学报, 1994, 21(1): 73-77.
[36]钱幼亭,周广和.麦类种质资源抗耐小麦黄矮病毒的田间鉴定技术研究[J].植物保护学报, 1986(03): 159-164.
[37]苗洪芹,陈巽桢,吴和平.承德春小麦黄矮病株系鉴定[J].河北农业大学学报, 1996, (19)3: 29-33.
[38]宋庆杰.黑龙江省小麦抗黄矮病研究初报[J].麦类作物, 1999, 19(3): 32-34.
[39]刘艳,钱幼亭,赵茂林等.多枝赖草及其转育后代对大麦黄矮病毒PAV和GAV株系的抗性研究[J].植物病理学报, 2002, 32(3): 247-251.
[40]刘小东,张增艳,刘艳等.抗病基因Bdv2抑制大麦黄矮病毒复制和运动的分子证据[J].遗传学报, 2005, 32(9): 942-947.
[41]张秦风,任芝英,金欣藻.关于小麦品种抗黄矮病毒的鉴定问题[J].植物保护, 1984, (6): 13-14.
[42]范绍强,谢咸升,郑王义等.小麦黄矮病遗传育种研究进展[J].中国生态农业学报, 2008, 16(1): 241-244.
[43]张增艳,辛志勇,陈孝等.源于L1的小麦抗黄矮病基因的特异PCR标记及辅助育种的研究[J].作物学报, 2002, 28(4): 486-491.
[44]马有志,徐琼芳,辛志勇等.抗黄矮病小麦种质的分子标记[J].作物学报, 1999, 25(4):433-436.
[45]武东亮.偃麦草(Agropyron pulcherrimum )黄矮病抗性向小麦的导入与鉴定[D].北京:中国农科院研究生院, 1997.
[46]李永丽,史洪中,陈利军等.转基因小麦抗大麦黄矮病毒研究进展[J].安徽农业科学, 2006, 34(21): 5451-5453.
[47]张增艳,辛志勇.抗黄矮病小麦生物技术育种研究进展[J].作物杂志,2005(5):4-7.
[48]王顺建,刘光荣,王向阳等.麦蚜天敌利用技术的研究[J].植保技术与利用,2002,22(10): 10-12.
[49]王随保.麦类病毒病及其防治[J].山西农业科学, 1984(9): 44-47.
[50]曹庆.河西种冬麦不是黄矮病大发生的唯一原因[J].甘肃农业科技, 1982(01):6-8.
[51]李晋梅. 1999年晋南小麦黄矮病流行原因及防治对策[J].农业科技通讯, 1999(10): 31.
[52]许仁林. PR-蛋白与高等植物的诱导抗性[J].病毒学杂志, 1989, (2): 121-123.
[53]江山,韩熹莱.植物病毒病化学防治的研究进展[J].中国病毒学, 1995, 10(l): 1-7.
[54]李在国,黄润秋,杨华铮等.化学合成植物病毒抑制剂研究进展[J].农药, 1998, 37(5): 3-6.
[55]安德荣.植物病毒化学防治的研究现状和所面临的问题[J].生命科学, 1994, 6(2): 15-18.
[56] Hohne C, Schuster G, Frommhold I. Growth Regulatory Effects of 5-Azadihydrouracil and Ribavirin [J]. Biochemistry Physiology Pflanzen, 1987, 182: 375-384.
[57]许良忠,郭玉晶,张书圣.植物病毒病化学防治的研究进展[J].青岛化工学院学报, 2000, 21(4): 293-297.
[58]许良忠,郭玉晶,张书圣.植物病毒防治剂盐酸吗啉呱的合成研究[J].沈阳化工学院学报, 2001, 15(2): 89-91.
[59]张华,宋宝安.杂环类植物抗病毒剂的研究进展[J].农药, 2002, 41(2): 6-9.
[60]陈齐斌,沈嘉样.抗植物病毒剂研究进展和面临的挑战与机遇[J].云南农业大学学报, 2005, 20(4): 505-512.
[61]赵玖华,尚佑芬,王升吉等.植物源抗病毒活性物质研究进展[J].石河子大学学报(自然科学版), 2004, 22(增刊).
[62]朱水方,裘维蕃.几种中草药抽提物对黄瓜花叶病毒引起的辣椒花叶病治疗作用初步研究[J].植物病理学报, 1989, 19(2): 123-128.
[63] Duggar BM, Armstrong JK. The effect of treating the virus of tobacco mosaic with the juice of various plants [J]. Annals of the Missouri Botanical Garden, 1925, (12): 359-366.
[64] Chen ZC, Antoniw JF, Lin Q, et al. A possible mechanism for the antiviral activity of pokeweed antiviral protein [J]. Physiol Mol Plant Pathol. 1993, 42(4): 249.
[65] Wyatt SD, Shepherd RJ. Isolation and characterization of a virus inhibitor from Phytolacca Americana [J]. Phytopathology, 1969, 59: 1987.
[66] Kuntz JE, Walker SC. Virus Inhibitor by Extracts of Spinach [J]. Phytopathology, 1947, 37: 561-579.
[67] Gianinazzi S, Kassanis B. Virus resistance induced in plants by polyacrylic acid [J]. J Gen Virology, 1974, 23: 1-9.
[68]林存銮,裘维蕃.一些植物抽提液对番茄花叶病毒病(TMV-T)的治疗作用[J].植物保护学报, 1987, 14(4): 217-219.
[69] Tsutomu I, Youichi T, Seiko I, et al. Formation of anti-plant viral protein by Mirabilis jalapa L. cells in suspension culture [J]. Plant Cell Reports, 1987, 6(3): 216-218.
[70] Susumu K, Tsutomu I, Seiko I, et al. A potent Plant virus inhibitor found in Mirabilis jalapa L. [J]. Annal Phytopathology Society of Japan, 1990, 56: 481-487.
[71] Verma HN, Awasthi LP. Isolation of the virus inhibitor from the root extract of Boerhaavia diffusa inducing systemic resistance in plants [J]. Canada Journal Botany, 1979, 57: 1214-1217.
[72]刘道贵,程伟星,汤牛根等.植物抽提物防治植物病毒病的研究[J].安徽农业科学, 2000, 28(6): 764-766.
[73]原征彦,冈田文雄等. [P].日本特开平6-199619, 1994.
[74] Chen ZC, White JF. Effect of pokeweed antiviral protein (PAP) on the infection of plant viruses [J]. Plant Pathology, 1991, 40: 612-620.
[75]吴云峰,曹让.几种药剂对病毒侵染和植物抗病性的影响[J].西北农业大学学报, 1999, 27(2): 1-5.
[76]许良忠,郭玉晶.植物病毒病化学防治研究进展[J].青岛化工学院学报(自然科学版), 2000, 21(4): 293-297.
[77]史清亮.施用PSB菌剂对番茄生长发育的影响及其防病(CMV)效果[J].山西农业科学, 1994, 22(增刊): 24-26.
[78]前田浩明.关于植物病毒抑制剂Retemin[J].今月农业, 1982, 26(6): 42-47.
[79]王贵,崔卫东. 5种抗病毒剂对烟草花叶病防效的研究[J].烟草科技, 1997, (5): 45.
[80]向固西.一种新的农用抗生素—宁南霉素[J].微生物学报, 1995, 35(5): 368-374.
[81]吴云峰.生物病毒农药筛选及应用[J].世界农业, 1995, 5: 35-36.
[82]李重九,侯玉霞.植物病毒病选择性治疗药剂的筛选研究[J].植物病理学报, 1997,27(4): 343-348.
[83]江山,郭雪柳,韩熹莱.一些抗植物病毒剂对烟草花叶病毒衣完蛋白体外聚合过程的影响[J].中国病毒学, 1996, 11(1): 77-79.
[84]杜春梅,吴元华,赵秀香等.天然抗植物病毒物质的研究进展[J].中国烟草学报, 2004, 10(1): 34-39.
[85] Schuster G, Horigklee W, Winter H, et al. Dioxoherxa-hydrotriazine, eneneuevoll synthetische antiphtoviral verbndung [J]. Zentralbl Bakteriol Mikrobiol Hyg, 1979, 1334: 64-69.
[86]黄荣茂.植物速丰剂对烟草普通花叶病(TMV)的防治研究[A].中国植物病理学会第三届代表大会暨学术年会论文集[C].北京: 1997.
[87] Goswam BB, Borke E, Sharma OK, et al. The broad spectrum antiviral agent ribabivirin inhibits capping of mRNA [J]. Biochemistry and Biophysics Research Community, 1979(89): 830-835.
[88]李重九,费著.丙酰紫草素对病毒的钝化作用[J].中国农业大学学报, 1997, 2(5): 47-50.
[89] French CJ, Elder M, Leggett F, et al. Flavonoids inhibit infectivity of tobacco mosaic virus [J]. Canadian Journal of Plant Pathology, 1991, 13: 1-8.
[90]张建新,吴云锋,樊兵.多羟基双萘醛提取物WCT抗病毒的生理病理学研究[J].植物病理学报, 2005, 35(6): 514-519.
[91]杨合同,王加宁.植物抗病反应中的蛋白磷酸化[J].山东科学, 2001, 14(2): 28-33.
[92]陈启建,刘国坤,吴祖建等.大蒜挥发油抗烟草花叶病毒机理[J].福建农业学报, 2006, 21(1): 24-27.
[93]沈建国.两种药用植物对植物病毒及三种介体昆虫的生物活性[D].福州:福建农林大学博士论文, 2005.
[94]侯玉霞,李重九,马立新.中草药中抗植物病毒TMV活性物质PZ作用机理研究[J].中国农业大学学报, 2000, 5(1): 21-24.
[95]龚国淑,张庆.梨树感染茎沟病毒后生长速度与内源激素的变化[J].西南农业学报, 2002, 15(2): 62-64.
[96]吴尔福.植物病毒及其防治[M].北京:中国科学技术出版社, 1996.
[97]费著,蔡祝南,刘仪等.抗病毒剂对烟草花叶病毒与烟草叶绿体互作的影响[J].植物保护, 1998 (2): 10-13.
[98]李在国,黄润秋.植物病毒防治剂的作用机制[J].植物保护, 1999, 25(3): 37-38.
[99]雷新云,李怀芳,裘维蕃.植物诱导抗性对病毒侵染的作用及诱导物质NS-83机制探讨[J].中国农业科学, 1987, 20(4): 1-51.
[100]雷新云,李怀芳,裘维蕃. 83增抗剂防治烟草病毒病研究进展[J].北京农业大学学报, 1990, 16(3): 241-248.
[101]刘学端,肖启明.植物源农药防治烟草花叶病机理初探[J].中国生物防治, 1997,13(3): 128-131.
[102]孙凤成,雷新云.耐病毒诱导剂88-D诱导珊西烟产生PR蛋白及对TMV侵染的抗性[J].植物病理学报, 1995, 25(4): 345-349.
[103]李兴红,贾月梅,商振清等. VA系统诱导烟草对TMV抗性与细胞内防御酶系统的关系[J].河北农业大学学报, 2003, 26(4): 21-24.
[104]刘联仁.烟草病虫防治[M].北京:科学出版社, 1998.
[105] Fodor J, Gullner G, Adam AL, et al. Local and systemic responses of antioxidants to tobacco mosaic virus infection and to Salicylicacid in tobacco (role in systemic acquired resistance) [J]. Plant Physiology, 1997(114): 1443-1451.
[106]殷培军,马志卿,冯俊涛等. VFB诱导烟草叶片生理生化变化与TMV抗性的关系[J].西北农业学报, 2005, 14(2): 91-95.
[107]吴尔福,张举仁,孙光荣. TA(TS剂)制剂防治玉米、粟红叶病的生理效应[J].上海农业学报, 1989, 5(1): 67-72.
[108]朱学文,李凤梅. RNA干扰及其在植物中的应用进展[J].长江大学学报(自科版)农学卷,2007, 4(3): 96-99.
[109]吴大利,侯岁稳.干扰RNA( RNAi)作用机理及其在植物细胞工程中的应用进展[J].中国生物工程杂志, 2006, 26(9) :84-90.
[110]赵明敏,赵健,郭永清等. RNA干扰及其在植物研究中的应用[J].农业生物技术科学,2006, 22 (4): 36-39.
[111]毛颖波,薛学义,陈晓亚.植物小RNA与RNA干扰:生物学功能与应用前景[J].2009,39(1): 31-43.
[112]燕飞,成卓敏. RNA干扰技术在几项研究领域的应用[J].中国生物工程杂志, 2004, 24 (8):14-19.
[113]王志坤,李文滨,刘珊珊. RNA干扰技术及在植物抗病研究中的应用[J].大豆科学, 2008, 27 (3):521-526.
[114] Abel PP , Nelson RS, De B, et al. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene [J]. Science, 1986, 232(4751): 738-742.
[115] Ma ZL, Yang HY, Wang R, et al. Construct hairpin RNA to fight against rice dwarf virus [J]. Acta Botanica Sinica, 2004, 46(3): 332-336.
[116] Wang MB, Abbott DC, Waterhouse PM. A single copy of a virus-derived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus [J].Mol. Plant Pathol, 2000, 1(6): 347-356.
[117]燕飞,张文蔚,肖红等.转病毒来源发夹RNA小麦表现对大麦黄矮病毒的抗性[J].遗传,2007,29(1): 97-102.
[118] Tenllado F, Llave C, Diaz-Ruiz JR. RNA interference as a new biotechnological tool for the control of virus diseases in plants [J]. Virus Research, 2004, 102: 85-96.
[119] Kalantidis K, Psaradakis S, Tabler M, et al. The occurrence of CMV-specific short RNAs in transgenic tobacco expressing virus-derived double-stranded RNA is indicative of resistance to the virus [J]. MPM I, 2002, 15(8): 826-833.
[120] Takahashi S, Komatsu K, Kagiwada S, et al.The efficiency of interference of Potato virus X infection depends on the target gene [J]. Virus Research, 2006( 116): 214-217.
[121] Ramachandran V, Padmanabhan C, Claude MF. Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells [J]. Proc Natl Acad Sci USA. 2002, 100(16), 9632–9636.
[122]牛颜冰,于翠,张凯等.瞬时表达黄瓜花叶病毒部分复制酶基因和番茄花叶病毒移动蛋白基因的dsRNA能阻止相关病毒的侵染[J].农业生物技术学报, 2004, 12(4):484-485.
[123]赵明敏,安德荣,黄广华等.瞬时表达靶向TMV外壳蛋白的siRNA能干扰病毒侵染[J].植物病理学报,2006, 36(1):35-40.
[124] Zhao MM, Yang XD, Lu HX, et al. siRNA-directed Interference of TMV RNA Accumulation in Tobacco Protoplast [J]. Acta Phytopathologica Sinica, 2006, 37(3): 284-288.
[125] Zhao MM, An DR, Huang GH, et al. A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection. Acta Biochim Biophys Sin (Shanghai). 2005, 37(4): 248-253.
[126]彭运生.关于提取叶绿素方法的比较研究[J].北京农业大学学报, 1992, 18 (3): 247- 250
[127] R.赫尔(编著),范在丰,李怀方等(译).马修斯植物病毒学[M].北京:科学出版社, 2007, 763-764.
[128]景永学.植病灵防治小麦黄矮病效果初报[J].甘肃农业科技, 1995, 5:39.
[129]杨洪元,廖旭辉.植物病毒病防治策略的研究进展[J].安徽农学通报, 2006, 12 (3): 74-76.
[130]赵明敏. siRNA干扰TMV侵染及其分子机理研究[D].杨凌:西北农林科技大学, 2005.