荒漠地区主要灌木类植物酚类物质含量动态规律及生物学评价
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摘要
本研究比较灌木类植物和紫花苜蓿的酚类物质含量和营养成分含量及动态变化规律、体外产气量、干物质消失率及瘤胃发酵参数。初步探讨了灌木类植物的酚类物质与营养成分、体外产气量、干物质消失率及瘤胃发酵参数之间的相关关系,为开发利用灌木类植物资源提供基础数据。研究结果表明:
荒漠地区几种主要灌木类植物中总多酚、简单酚、单宁和缩合单宁的平均含量分别为75.76g/kgDM～101.78g/kgDM、12.98g/kgDM～19.88g/kgDM、61.07g/kgDM～86.09g/kgDM和25.66g/kgDM～46.66g/kgDM,其除了简单酚以外,上述物质在羊柴中含量最高,木地肤中含量最低。在生长期间灌木类植物总多酚、单宁和缩合单宁含量有“V”字型变化或随生长期逐渐上升,而且生长期间差异极显著,几种灌木酚类物质含量均高于紫花苜蓿。总多酚含量与简单酚、单宁和缩合单宁之间显著正相关(r=0.687、r=0.953、r=0.908,p〈0.01)。简单酚与单宁显著正相关(r=0.610)。单宁与缩合单宁显著正相关(r=0.922,p〈0.01)。
荒漠地区主要灌木类植物不同生长期粗蛋白、NDF、ADF、ADL、粗脂肪、粗灰分、钙和磷平均含量分别为7.80%～18.45%、38.10%～49.91%、29.34%～37.69%、3.67%～10.10%、1.77%～6.59%、5.40%～17.48%、0.55%～2.47%和0.14%～0.30%,随着生长期的延长,各灌木类植物中的粗蛋白含量逐渐下降,平均含量最高的是中间锦鸡儿,NDF和ADF含量动态变化与粗蛋白相反,平均含量最高的是大白柠条。
体外试验结果表明,荒漠地区灌木类植物中总多酚、单宁和缩合单宁含量与各时间点发酵产气量之间有显著的相关关系(p〈0.05),与总产气量、干物质消失率相关性较弱。四种酚类物质与氨氮呈显著负相关(p〈0.01);总多酚和缩合单宁与菌体蛋白呈显著负相关关系(p〈0.05),与其他发酵参数相关性较弱。
In this study,the content of polyphenol and nutrient content and its the dynamic law, gas production in vitro, dry matter disappearance and ruminal ferment parameters of the main desert shrubs and alfalfa in different growthing stages were compared. The relationship of nutritional components, gas production in vitro, dry matter disappearance and rumen fermentation parameters compared with the polyphenol of shrubs were researched respectively. It was provided the basic data for developing and utilizating desert shrubs resources.The results showed that:
The average content of total polyphenol, simple phenols, tannin and condensed tannin of several main desert shrubs were 75.76 g/kgDM～101.78g/kgDM, 12.98g/kgDM～19.88g/kgDM, 61.07g/kgDM～86.09g/kgDM and 25.66g/kgDM～46.66g/kgDM respectively, above polyphenol Contents in Hedy sarum leave mongolicum was the highest and Kochia prostrate(L.)Schrad was the lowest except simple phenols. The content of polyphenols, tannins and condensed tannin had the "V" shape change or increased and highly signicant.on the During growthing stages, those were higher than alfalfa.There was a highly significantly positive correlation between the total polyphenols and simple phenols, tannins, condensed tannin(r=0.687, r=0.953, r=0.908) .The simple phenols and tannins was a significant positive correlatetion(r=0.610). tannins and condensed tannin was a highly significant positive correlation(r = 0.922).
The average content of CP, NDF, ADF, ADL, EE, Ach, Ca and P of several main desert shrubs were 7.80%～18.45%, 33.44%～49.91%, 23.00%～37.69%, 3.67%～10.10%, 1.77%～6.59%, 5.40%～17.48%, 0.55%～2.47% and 0.14%～0.30% respectively,The content of Crude protein ( CP )in shrubs was decreased during the growing stages. Caragana Intermedia had the highest CP concentration, Neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents were increased and Caragana korshinskii Kom had the lowest.
The Test in vitro showed that The total polyphenols, tannins, condensed tannins in desert shrubs compared with gas production at all time points had a significant correlation respectively(p〈0.05). The four kinds of polyphenols and NH3-N were highly significant negative correlation(p〈0.01). The total phenol,condensed tannins compared with BCP had significantly negative correlation respectively(p〈0.05),And a less correlation in other fermentation parameters.
荒漠地区几种主要灌木类植物中总多酚、简单酚、单宁和缩合单宁的平均含量分别为75.76g/kgDM～101.78g/kgDM、12.98g/kgDM～19.88g/kgDM、61.07g/kgDM～86.09g/kgDM和25.66g/kgDM～46.66g/kgDM,其除了简单酚以外,上述物质在羊柴中含量最高,木地肤中含量最低。在生长期间灌木类植物总多酚、单宁和缩合单宁含量有“V”字型变化或随生长期逐渐上升,而且生长期间差异极显著,几种灌木酚类物质含量均高于紫花苜蓿。总多酚含量与简单酚、单宁和缩合单宁之间显著正相关(r=0.687、r=0.953、r=0.908,p〈0.01)。简单酚与单宁显著正相关(r=0.610)。单宁与缩合单宁显著正相关(r=0.922,p〈0.01)。
荒漠地区主要灌木类植物不同生长期粗蛋白、NDF、ADF、ADL、粗脂肪、粗灰分、钙和磷平均含量分别为7.80%～18.45%、38.10%～49.91%、29.34%～37.69%、3.67%～10.10%、1.77%～6.59%、5.40%～17.48%、0.55%～2.47%和0.14%～0.30%,随着生长期的延长,各灌木类植物中的粗蛋白含量逐渐下降,平均含量最高的是中间锦鸡儿,NDF和ADF含量动态变化与粗蛋白相反,平均含量最高的是大白柠条。
体外试验结果表明,荒漠地区灌木类植物中总多酚、单宁和缩合单宁含量与各时间点发酵产气量之间有显著的相关关系(p〈0.05),与总产气量、干物质消失率相关性较弱。四种酚类物质与氨氮呈显著负相关(p〈0.01);总多酚和缩合单宁与菌体蛋白呈显著负相关关系(p〈0.05),与其他发酵参数相关性较弱。
In this study,the content of polyphenol and nutrient content and its the dynamic law, gas production in vitro, dry matter disappearance and ruminal ferment parameters of the main desert shrubs and alfalfa in different growthing stages were compared. The relationship of nutritional components, gas production in vitro, dry matter disappearance and rumen fermentation parameters compared with the polyphenol of shrubs were researched respectively. It was provided the basic data for developing and utilizating desert shrubs resources.The results showed that:
The average content of total polyphenol, simple phenols, tannin and condensed tannin of several main desert shrubs were 75.76 g/kgDM～101.78g/kgDM, 12.98g/kgDM～19.88g/kgDM, 61.07g/kgDM～86.09g/kgDM and 25.66g/kgDM～46.66g/kgDM respectively, above polyphenol Contents in Hedy sarum leave mongolicum was the highest and Kochia prostrate(L.)Schrad was the lowest except simple phenols. The content of polyphenols, tannins and condensed tannin had the "V" shape change or increased and highly signicant.on the During growthing stages, those were higher than alfalfa.There was a highly significantly positive correlation between the total polyphenols and simple phenols, tannins, condensed tannin(r=0.687, r=0.953, r=0.908) .The simple phenols and tannins was a significant positive correlatetion(r=0.610). tannins and condensed tannin was a highly significant positive correlation(r = 0.922).
The average content of CP, NDF, ADF, ADL, EE, Ach, Ca and P of several main desert shrubs were 7.80%～18.45%, 33.44%～49.91%, 23.00%～37.69%, 3.67%～10.10%, 1.77%～6.59%, 5.40%～17.48%, 0.55%～2.47% and 0.14%～0.30% respectively,The content of Crude protein ( CP )in shrubs was decreased during the growing stages. Caragana Intermedia had the highest CP concentration, Neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents were increased and Caragana korshinskii Kom had the lowest.
The Test in vitro showed that The total polyphenols, tannins, condensed tannins in desert shrubs compared with gas production at all time points had a significant correlation respectively(p〈0.05). The four kinds of polyphenols and NH3-N were highly significant negative correlation(p〈0.01). The total phenol,condensed tannins compared with BCP had significantly negative correlation respectively(p〈0.05),And a less correlation in other fermentation parameters.
引文
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