柳蒿芽醇提物抗氧化作用研究
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摘要
柳蒿芽是一种药食两用野生植物资源,自古就有其对止血消炎、镇咳化痰、开胃健脾、散寒除湿等药用的记载。柳蒿芽富含的黄酮类化合物具有生物抗氧化性、抗衰老、治疗心脑血管疾病、抗菌消炎等多种保健功能的主要生理活性物质。本文以柳蒿芽为原料,主要研究了柳蒿芽醇提物的抗氧化作用。主要试验结果如下：
1.分别对柳蒿芽醇提物的总抗氧化性、抗脂质过氧化能力、OH·及02-·的能力进行研究,结果表明,柳蒿芽醇提物具有良好的体外抗氧化活性。
2.柳蒿芽醇提物干预组小鼠比衰老模型组小鼠血清、肝脏、脑中SOD、GSH-Px含量明显提高,MDA含量明显降低,与衰老模型组比较,差异有显著性(P<0.05,P<0.01),柳蒿芽醇提物可以较好提高衰老小鼠体内抗氧化能力。
3.柳蒿芽醇提物可明显减弱小鼠脾脏细胞荧光强度,抑制细胞内活性氧的产生,与阳性对照组比较,差异具有显著性(P<0.05,P<0.01)。
4.加入柳蒿芽醇提物组的小鼠脾脏细胞比对照组小鼠脾脏细胞DNA氧化损伤率明显降低,损伤率从83.5%分别降为62.5%、53.0%、44.5%,差异有显著性(P<0.05,P<0.01),柳蒿芽醇提物干预组DNA拖尾长度比对照组显著降低(p<0.05),柳蒿芽醇提物对小鼠脾脏细胞DNA氧化损伤有防护作用。。
Artemisin selengensis is a plant with high pharmaceutical and edible value. Since ancient times its functions of Hemostasis, anti-inflammatory, antitussive phlegm, appetizers strengthening spleen, cold-dispelling the desiccant have been recorded. Flavonoids compounds in Artemisin selengensis is one of the most important physiological and active matters which has a better capacity of anti-oxidation and anti-caducity,curing blood vessel diseases, antisepsis and anti-inflammation and so on. The anti-oxidative capacity of ethanol extract froom Artemisin selengensis was mainly studied in this paper and the results were as follows:
1. The anti-oxidative character, Anti-lipid peroxidation, removal capacity of·OH and O2-·of ethanol extract froom Artemisin selengensis were studied,the result showed that flavonoids compounds in Artemisin selengensis have good oxidation resistance.
2. The activities of SOD and GSH-Px in serum,liver and brain in aged mice of the group which treated with ethanol extract froom Artemisin selengensis were significantly higher and the content of MDA was significantly lower than the aged model group(P<0.05, P＜0.01). Ethanol extract froom Artemisin selengensis had good antioxidant effect in aged mice.
3. The ethanol extract froom Artemisin selengensis could significantlly increase cell activity and attenuate active oxygen production of splenic cells of mice(P<0.05, P＜0.01).
4. The ethanol extract froom Artemisin selengensis could significantlly increase survival rate of splenic cells of mice and the quantity of comet splenic cells of the group which treated with ethanol extract froom Artemisin selengensis was significantly lower than the positive control group(P<0.05, P＜0.01),and was reduced from 83.5% to 62.5%,53.0%,44.5%. The comet length of the group which treated with ethanol extract froom Artemisin selengensis was significantly lower than the positive control group(P<0.05). The ethanol extract froom Artemisin selengensis could effectively decrease the oxidative DNA damage induced by H2O2.
1.分别对柳蒿芽醇提物的总抗氧化性、抗脂质过氧化能力、OH·及02-·的能力进行研究,结果表明,柳蒿芽醇提物具有良好的体外抗氧化活性。
2.柳蒿芽醇提物干预组小鼠比衰老模型组小鼠血清、肝脏、脑中SOD、GSH-Px含量明显提高,MDA含量明显降低,与衰老模型组比较,差异有显著性(P<0.05,P<0.01),柳蒿芽醇提物可以较好提高衰老小鼠体内抗氧化能力。
3.柳蒿芽醇提物可明显减弱小鼠脾脏细胞荧光强度,抑制细胞内活性氧的产生,与阳性对照组比较,差异具有显著性(P<0.05,P<0.01)。
4.加入柳蒿芽醇提物组的小鼠脾脏细胞比对照组小鼠脾脏细胞DNA氧化损伤率明显降低,损伤率从83.5%分别降为62.5%、53.0%、44.5%,差异有显著性(P<0.05,P<0.01),柳蒿芽醇提物干预组DNA拖尾长度比对照组显著降低(p<0.05),柳蒿芽醇提物对小鼠脾脏细胞DNA氧化损伤有防护作用。。
Artemisin selengensis is a plant with high pharmaceutical and edible value. Since ancient times its functions of Hemostasis, anti-inflammatory, antitussive phlegm, appetizers strengthening spleen, cold-dispelling the desiccant have been recorded. Flavonoids compounds in Artemisin selengensis is one of the most important physiological and active matters which has a better capacity of anti-oxidation and anti-caducity,curing blood vessel diseases, antisepsis and anti-inflammation and so on. The anti-oxidative capacity of ethanol extract froom Artemisin selengensis was mainly studied in this paper and the results were as follows:
1. The anti-oxidative character, Anti-lipid peroxidation, removal capacity of·OH and O2-·of ethanol extract froom Artemisin selengensis were studied,the result showed that flavonoids compounds in Artemisin selengensis have good oxidation resistance.
2. The activities of SOD and GSH-Px in serum,liver and brain in aged mice of the group which treated with ethanol extract froom Artemisin selengensis were significantly higher and the content of MDA was significantly lower than the aged model group(P<0.05, P＜0.01). Ethanol extract froom Artemisin selengensis had good antioxidant effect in aged mice.
3. The ethanol extract froom Artemisin selengensis could significantlly increase cell activity and attenuate active oxygen production of splenic cells of mice(P<0.05, P＜0.01).
4. The ethanol extract froom Artemisin selengensis could significantlly increase survival rate of splenic cells of mice and the quantity of comet splenic cells of the group which treated with ethanol extract froom Artemisin selengensis was significantly lower than the positive control group(P<0.05, P＜0.01),and was reduced from 83.5% to 62.5%,53.0%,44.5%. The comet length of the group which treated with ethanol extract froom Artemisin selengensis was significantly lower than the positive control group(P<0.05). The ethanol extract froom Artemisin selengensis could effectively decrease the oxidative DNA damage induced by H2O2.
引文
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