双价转基因抗虫棉的获得及其抗虫性与生理生化特征
|
摘要
2001年我国抗虫棉种植面积已达160万公顷，但几乎全部是单价Bt基因抗虫棉。研究表明，单价Bt基因抗虫棉在大面积推广应用后，棉铃虫可能会对其产生抗性。为此，将双价基因(GFMcry IA+CpTI)导入优良棉花品种，获得新类型抗虫种质，加速双价转基因抗虫棉新品种培育，并对其遗传规律和抗虫性表达等进行全面研究具有重要意义。
本研究通过农杆菌介导法和花粉管通道法转化获得双价转基因抗虫棉；通过卡那霉素筛选、ELISA检测、生物鉴定以及PCR扩增、Southern杂交等方法进行筛选鉴定、时空表达研究、逆境的影响以及分子验证等；根据其后代分离情况，研究其遗传行为；根据相应酶活性、激素、叶绿素等的测定研究其生化特征。
在前期工作的基础上，完善了农杆菌介导法和花粉管通道法遗传转化技术，优化了主要技术参数，拓宽了棉花基因型范围，实现了部分优良受体材料高效转化，初步建立了规模化转基因技术体系，并获得了大量双价转基因抗虫棉新材料。研究了部分双价转基因抗虫棉抗性分离情况，对双价转基因抗虫棉不同发育时期的抗虫性、相同时期不同器官的抗虫性、不同龄期棉铃虫幼虫对其敏感性以及不同世代棉铃虫的敏感性等进行了分析。仿照我国主要棉区的某些特定或易变气候特点、环境条件，研究了温度变化、干旱处理、盐碱条件等对双价转基因抗虫棉抗性的影响。在不同生育期取样，研究了双价转基因抗虫棉超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)等抗氧化系统酶活性，研究了丙二醛(MDA)、脯氨酸(pro)等生化物质、吲哚乙酸(IAA)与脱落酸(ABA)等内源激素以及叶绿素等的含量变化规律。
通过本项研究发现，采用嫁接技术，可使转基因再生植株成活率达到90%以上，可有效提高转化效率。分子检测结果显示，双价基因已整合到棉花染色体基因组中。遗传分析数据表明，花粉管通道法转化所获得的转基因抗虫材料多拷贝比例可能偏高，多数后代材料不能按照孟德尔遗传规律分离。生物鉴定结果说明，双价转基因抗虫棉同样具有时空表达性，在3代棉铃虫发生期间其抗虫性也有所
下降，在4代棉铃虫发生期间又有所回升，形成一个波谷，但双价基因抗虫棉的
抗虫性下降幅度小于单价Bt基因抗虫棉，其抗虫稳定性优于单价Bt基因抗虫棉；
在棉花生长中后期，双价基因抗虫棉生殖器官如花、棉蕾等组织仍具有较强抗虫
性。试验证明，在实际生产中，短时间环境条件的突然变化如温度等不影响双价
转基因抗虫棉的抗虫性表达。双价转基因抗虫棉叶片ABA含量低于常规对照品
种，IAA含量高于常规品种，表明双价转基因抗虫棉叶片中期生长旺盛，有利于
提高光合强度，增加光合产物，提高生物产量。
研究结果对双价转基因抗虫棉在生产中的推广应用及其综合防治等具有一定
的指导意义。
In 2001, insect-resistant transgenic cotton were grew in 1.6 million ha, and it was about 40% of the planting area of Chinese cotton, but they were almost Bt transgenic cotton. Studies showed bollworm possibly produced some resistance to Bt transgenic cotton after massive planting. Therefore, it was very important of the introduction the synthesized double-gene, GFM CryIA gene and modified CpTI gene, to the elite cotton (G. hirsutum L.) and the production of new style resources of insect-resistance were obtained, cultivation of new style insect-resistant transgenic cotton and rules of inheritance and their insect-resistance and main characters related to physiology and biochemistry were studied.
Insect-resistant transgenic cotton plants harbouring double-gene were obtained by the pollen-tube pathway transformation system and the agrobacterium tumefaciens-mediated transformation system. Their selection and identification, studies on space-time expression of the target-gene, influences from adversities, molecular analysis, etc. were proceeded by kanamycin selection, ELISA detection, bio-identification, PCR amplification, Southern Blotting; their inheritance rules was resulted from the separation results of their offspring, their physiological and biochemical characters was studied by activities of enzymes, contents of endogenous hormones, the trends of contents variations of MDA, proline, chlorophyll ,etc.
Based on my previous works, the techniques of the pollen tube pathway transformation system and agrobacterium tumefaciens-mediated transformation system were improved, main parameters of technical were optimized, the genotypes of the cotton acceptors were widen, a high
efficiency of transformation was achieved, a technical system of transformation on a large-scale was established and a lot of transgenic cotton plants harboring double-gene were acquired. In my experiment, I studied the separation results of the insect-resistance to bollworm of part of the transgenic cotton plants, analyzed and compared their insect-resistance at different growth stages and their insect-resistance of different organs at same growth stages, analyzed and compared the sensitivities of bollworm of different instar of larvae, sensitivities of different generations
bollworms to the transgenic cotton. Leaves. Imitated some special and variable characters of climate and condition of environment, I studied the influences of variations of temperature and the treatments by drought and NaCl, etc to the transgenic cottons. I Studied the activities of SOD, CAT, POD and the trends of contents variations of MDA, proline and chlorophyll and endogenous ABA,IAA of the transgenic cottons.
The results showed the surviving rates of the regeneration transgenic plants of different varieties exceed 90% by grafting. PCR and Southern blotting detection results showed the existence of the target gene and indicated the Bt and CpTI genes were inserted into cotton genome. The results of inheritance indicated a lot of transgenic cottons harboring double-gene by the pollen tube pathway transformation did not fit to Mendel Rules that resulted from many copies of insert-gene in the transgenic cotton plants. Same as Bt transgenic cotton, the space-time expression of insect-resistance of the transgenic cotton harboring double-gene to bollworm was different in different times and organs, their insect-resistance had a descending trend during 3rd generation, and afterward, it began to rise during 4rd generation, but the descending extent of the transgenic cottons was less than that of Bt transgenic cotton, the stability of their insect-resistance to bollworm was more than that of Bt transgenic cotton. In the middle-last days of growth, the insect-resistances of the flowers and buds of the transgenic cottons were more than that of its leaves. The research results showed the insect-resistance of the transgenic cottons did not affected by the changes of temperature in the process of growth, and sudden-variations of environment did not influenced the expression
本研究通过农杆菌介导法和花粉管通道法转化获得双价转基因抗虫棉；通过卡那霉素筛选、ELISA检测、生物鉴定以及PCR扩增、Southern杂交等方法进行筛选鉴定、时空表达研究、逆境的影响以及分子验证等；根据其后代分离情况，研究其遗传行为；根据相应酶活性、激素、叶绿素等的测定研究其生化特征。
在前期工作的基础上，完善了农杆菌介导法和花粉管通道法遗传转化技术，优化了主要技术参数，拓宽了棉花基因型范围，实现了部分优良受体材料高效转化，初步建立了规模化转基因技术体系，并获得了大量双价转基因抗虫棉新材料。研究了部分双价转基因抗虫棉抗性分离情况，对双价转基因抗虫棉不同发育时期的抗虫性、相同时期不同器官的抗虫性、不同龄期棉铃虫幼虫对其敏感性以及不同世代棉铃虫的敏感性等进行了分析。仿照我国主要棉区的某些特定或易变气候特点、环境条件，研究了温度变化、干旱处理、盐碱条件等对双价转基因抗虫棉抗性的影响。在不同生育期取样，研究了双价转基因抗虫棉超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)等抗氧化系统酶活性，研究了丙二醛(MDA)、脯氨酸(pro)等生化物质、吲哚乙酸(IAA)与脱落酸(ABA)等内源激素以及叶绿素等的含量变化规律。
通过本项研究发现，采用嫁接技术，可使转基因再生植株成活率达到90%以上，可有效提高转化效率。分子检测结果显示，双价基因已整合到棉花染色体基因组中。遗传分析数据表明，花粉管通道法转化所获得的转基因抗虫材料多拷贝比例可能偏高，多数后代材料不能按照孟德尔遗传规律分离。生物鉴定结果说明，双价转基因抗虫棉同样具有时空表达性，在3代棉铃虫发生期间其抗虫性也有所
下降，在4代棉铃虫发生期间又有所回升，形成一个波谷，但双价基因抗虫棉的
抗虫性下降幅度小于单价Bt基因抗虫棉，其抗虫稳定性优于单价Bt基因抗虫棉；
在棉花生长中后期，双价基因抗虫棉生殖器官如花、棉蕾等组织仍具有较强抗虫
性。试验证明，在实际生产中，短时间环境条件的突然变化如温度等不影响双价
转基因抗虫棉的抗虫性表达。双价转基因抗虫棉叶片ABA含量低于常规对照品
种，IAA含量高于常规品种，表明双价转基因抗虫棉叶片中期生长旺盛，有利于
提高光合强度，增加光合产物，提高生物产量。
研究结果对双价转基因抗虫棉在生产中的推广应用及其综合防治等具有一定
的指导意义。
In 2001, insect-resistant transgenic cotton were grew in 1.6 million ha, and it was about 40% of the planting area of Chinese cotton, but they were almost Bt transgenic cotton. Studies showed bollworm possibly produced some resistance to Bt transgenic cotton after massive planting. Therefore, it was very important of the introduction the synthesized double-gene, GFM CryIA gene and modified CpTI gene, to the elite cotton (G. hirsutum L.) and the production of new style resources of insect-resistance were obtained, cultivation of new style insect-resistant transgenic cotton and rules of inheritance and their insect-resistance and main characters related to physiology and biochemistry were studied.
Insect-resistant transgenic cotton plants harbouring double-gene were obtained by the pollen-tube pathway transformation system and the agrobacterium tumefaciens-mediated transformation system. Their selection and identification, studies on space-time expression of the target-gene, influences from adversities, molecular analysis, etc. were proceeded by kanamycin selection, ELISA detection, bio-identification, PCR amplification, Southern Blotting; their inheritance rules was resulted from the separation results of their offspring, their physiological and biochemical characters was studied by activities of enzymes, contents of endogenous hormones, the trends of contents variations of MDA, proline, chlorophyll ,etc.
Based on my previous works, the techniques of the pollen tube pathway transformation system and agrobacterium tumefaciens-mediated transformation system were improved, main parameters of technical were optimized, the genotypes of the cotton acceptors were widen, a high
efficiency of transformation was achieved, a technical system of transformation on a large-scale was established and a lot of transgenic cotton plants harboring double-gene were acquired. In my experiment, I studied the separation results of the insect-resistance to bollworm of part of the transgenic cotton plants, analyzed and compared their insect-resistance at different growth stages and their insect-resistance of different organs at same growth stages, analyzed and compared the sensitivities of bollworm of different instar of larvae, sensitivities of different generations
bollworms to the transgenic cotton. Leaves. Imitated some special and variable characters of climate and condition of environment, I studied the influences of variations of temperature and the treatments by drought and NaCl, etc to the transgenic cottons. I Studied the activities of SOD, CAT, POD and the trends of contents variations of MDA, proline and chlorophyll and endogenous ABA,IAA of the transgenic cottons.
The results showed the surviving rates of the regeneration transgenic plants of different varieties exceed 90% by grafting. PCR and Southern blotting detection results showed the existence of the target gene and indicated the Bt and CpTI genes were inserted into cotton genome. The results of inheritance indicated a lot of transgenic cottons harboring double-gene by the pollen tube pathway transformation did not fit to Mendel Rules that resulted from many copies of insert-gene in the transgenic cotton plants. Same as Bt transgenic cotton, the space-time expression of insect-resistance of the transgenic cotton harboring double-gene to bollworm was different in different times and organs, their insect-resistance had a descending trend during 3rd generation, and afterward, it began to rise during 4rd generation, but the descending extent of the transgenic cottons was less than that of Bt transgenic cotton, the stability of their insect-resistance to bollworm was more than that of Bt transgenic cotton. In the middle-last days of growth, the insect-resistances of the flowers and buds of the transgenic cottons were more than that of its leaves. The research results showed the insect-resistance of the transgenic cottons did not affected by the changes of temperature in the process of growth, and sudden-variations of environment did not influenced the expression
引文
1．曹孟良 农杆菌介导的水稻遗传转化及自我调控延缓叶片衰老系统在水稻中的表现：[博士论文]．武汉：华中农业大学，1998
2．陈奇思，田明军，吴云康棉花生育规律与优质高产高效栽培．中国农业出版社，1997
3．陈思学 农杆菌与植物细胞的相互作用，生物学通报，1993，28(3)：13
4．陈思学，黄祥辉 农杆菌与植物细胞识别研究的进展，生物学杂志，1995，5：12～14
5．陈志贤，N.L.Trolinder, J.R.Goodin棉花细胞悬浮培养胚胎发生和植株再生某些特性的研究，中国农业科学．1987，20(5)：6～11
6．陈志贤，Danny J．Llewellyn，范云六，李淑君，郭三堆，焦改丽，赵俊侠 利用农杆菌介导法转移tfdA基因获得可遗传的抗2，4—D棉株。中国农业科学．1994，27(2)：31～37
7．崔洪志，郭三堆，Bt毒蛋白抗虫植物基因工程研究，农业生物技术学报，1998，6(2)：166～172
8．丁群星，谢友菊，戴景瑞，米景九，李太元，田颖川，乔利亚，莽克强，刘宝兰，王音，冯平章 用子房注射法将Bt毒蛋白基因导入玉米的研究．中国科学(B辑)．1993，23(7)：707～713
9．董合忠，焦改丽2，4—D、KT对棉花愈伤组织的诱导和体细胞胚胎发生的影响，华北农学报，1993，8(3)：87～91
10．段俊，梁承邺，张明永玉米细胞质雄性不育与膜脂过氧化的关系．植物生理学通讯．1996，32(5)：331-334
11．段留生，张一，何钟佩，翟志席 生长素和细胞分裂素对棉花幼苗根系顶端优势的调控作用，棉花学报，2001，13(6)：330-333
12．范月仙，张述义，李生泉 棉花苗期抗冷性与可溶性蛋白含量增加的研究，山西农业大学学报，1995，15(1)：56～58
13．范月仙，张述义 棉苗抗冷性与POD活性变化关系的研究，棉花学报，1995，7(3)：172～174
14．范云六，张春义，贾继增 我国农作物生物技术的成就与展望，世界科技研究与发展，1999，21(1)，11～15
15．傅荣昭，孙勇如，贾士荣 植物遗传转化技术手册．北京：中国科学技术出版社，1994．35～39
16．高丽锋，胡志昂，王红新，阳文龙 根癌菌结瘤因子的结构和功能，生命科学，2002，14(1)：17～19
17．何钟佩 主编．农作物化学控制实验指导．北京农业大学出版社，1990：60-68
18．何钟佩 主编．作物激素生理及化学控制．中国农业大学出版社．1997：226～230
19．侯彩霞，汤章城 细胞相容性物质的生理功能及其作用机制．植物生理学通讯．1999，35(1)
20．侯文胜，郭三堆，路明 利用转基因技术进行植物遗传改良．生物技术通报．2002，1：10～15
21．胡延章，徐云剑，江国英，朱玉贤 转Bank I T4基因烟草的抗虫性，植物学报，2000，42(7)：732～735
22．耿飒，麻密 ipt基因定位表达对转基因烟草育种的影响．植物学报．2000，42(2)：217～220
23．巩万奎，喻树迅，陈志贤，吴家和，余学科 葡萄糖氧化酶基因在棉花中的高效转化．棉花学报．1999，11(5)：241～246
24．郭三维 植物BT抗虫基因工程研究进展．中国农业科学．1995，28(5)：8～13
25．郭三堆，崔洪志，夏兰芹，武东亮，倪万潮，张震林，张保龙，徐英俊 双价抗虫转基因棉花研究，中国农业科学，1999，32(3)：1～7
26．郝联芳，刘养浩 陆地棉基因转移技术研究初报，陕西师范大学学报(自然科学版)，1997，21(1)：83～86
27．郝秀英，李仁敬 用基因枪转化新疆陆地棉新陆早4号，新疆农业科学，1999，6：255～256
28．黄骏麒，钱思颖，刘桂玲，翁坚，曾以申，周光宇 外源海岛棉DNA导致陆地棉性状的变异．遗传学报，1981，8(1)：56～62
29．贾士荣，郭三堆，安道昌 棉花转基因，北京，科学出版社，2001
30．贾士荣 T-DNA转移机理，植物生理学通讯，1994，30(4)：306～311
31．焦改丽等，郭三堆，李淑君，陈志贤，赵俊侠 棉花遗传转化和植株再生的研究，华北农学报，1997，12(2)：82～85
32．李宝平，赵俊侠等 关键因子对棉花利用农杆菌介导法导入外源基因的影响．作物学报．2001，27(1)：80～84
33．李付广，周勇，刘传亮，李凤莲，李秀兰 影响陆地棉遗传转化的外部因子研究，西北植物学报，1999，19(3)：387—392
34．李国胜，刘玉乐，朱峰等基因工程雄性不育烟草的获得．植物学报，1995，37(8)：659～660
35．李洪清，李美茹 影响农杆菌介导植物基因转化的因素问题，植物生理学通讯，1999，35(2)：145～151
36．李克勤，王喆之，张大力，郭德志，郝联芳，张苏峰，沈志刚 陆地棉组织细胞培养的研究(J)。西北植物学报，1991，(11)：144—153
37．李卫，郭光沁，郑国锠 根癌农杆菌介导遗传转化研究的若干新进展，科学通报，2000，
45(8)：798～807
38．李晓，王学德，朱玉贤，卢迎春，赵向前 农杆菌真空渗透法转化花粉获得棉花转acsB基因植株，作物学报，2002，28(5)：709～712
39．李燕娥，朱祯，陈志贤，吴霞，王伟，李淑君，朱玉，焦改丽，吴家和，徐鸿林，范小平，孟晋红，肖桂芳，李向辉 豇豆胰蛋白酶抑制转基因棉花的获得．棉花学报．1998，10(5)：237～243
40．李燕娥，焦改丽，吴家和，石跃进，吴霞，范小平，孟晋红，李淑君，陈志贤，郭三堆，朱祯，田颖川 棉花农杆菌介导高效转化体系．中国棉花．2000，27(5)：10～11
41．李艳红，肖兴国，赵广荣，聂秀玲，张爱民，阎隆飞 将新的人工雄性不育基因导入小麦栽培品种的研究初报．农业生物技术学报，1999，7(3)：255～258
42．李子银，胡会庆 农杆菌介导的植物遗传转化进展，生物工程进展，1998，18(1)：22～26，16
43．李宗霆，周燮 植物激素及其免疫检测技术．南京：江苏科学技术出版社，1996
44．立洪清，李美茹 影响农杆菌介导植物基因转化的因素问题．植物生理学通讯，1999，35(2)145-151
45．廖祥儒，贺普超，朱新 玉米素对盐渍政葡萄叶圆片H_2O_2清除系统的影响．植物学报．1997，39(7)：641～646
46．刘春明，姚敦义 陆地棉体细胞胚发生及其细胞组织学研究，植物学报，1991，336(5)：378—384
47．刘志，袁小玲，张天真 获得多价转基因作物的策略，遗传，2001，23(2)：132～136
48．刘志学，马向前，何艺园，徐亚男，叶明鸣，唐克轩 农杆菌介导遗传转化中辅助处理方法的改良，复旦学报(自然科学版)，1999，38(5)：601～604
49．刘博林，岳绍先，胡乃璧，李小兵，翟文学，李诺，朱荣焕，朱立煌，毛大璋，周佩珍 龙葵Atrazine抗性基因向大豆叶绿体的转移及在转基因植株中的表达．中国科学B辑．1989，7：699～705
50．刘道宏 植物叶片的衰老．植物生理学通讯．1983，(2)：14～19
51．马庆虎，张韧，Higgins Thomas J V 异戊烯基转移酶基因在转基因烟草中的特异性表达．植物学报．1994，36(5)：339～344
52．毛树春，韩迎春，宋美珍，张朝军，范正义 中棉所29栽培技术及其生理特性研究棉花学报，2001，3(2)：82～86
53．明小天，苑华毅，陈章良 通过基因枪提高根癌农杆菌转化水稻的效率．植物学报．2001，43(1)：72～76
54．萨姆布鲁克J，弗里奇EF,曼尼阿蒂斯T 分子克隆实验指南，第二版．北京科技出版社．金冬雁，犁孟枫译，1992．19～34
55．苏金，J．Targolli，吴乃虎，吴瑞 在转基因植物中实现外源基因最佳表达的途径，生物工程进展，1999，19(4)：3～6
56．沈成国，张福锁，毛达如 植物叶片衰老过程中基因的表达与调控．植物生理学通讯．1998，34(4)：304～311
57．汤学军，王康 激动素和抗坏血酸保护受冷害功细胞膜和促进SOD合成的效应．植物学报(增刊)．1993，45～49
58．万丙良，张献龙 基因枪介导转化水稻花粉获得转基因植株．农业生物技术学报．2000，8(4)：332
59．魏伟，钱迎倩，马克平 转基因作物与其野生亲缘种间的基因流．植物学报1999，41(4)：343-348
60．王从丽，陆柏方，张学成，李广旭，王劲波，李延军，彭友良，潘申权 农杆菌—植物间基因转移的分子基础，生命科学，2002，14(1)：1～5
61．王长海，蓝海燕 利用花粉管通道法将外源质粒DNA注入棉花的改良方法．棉花学报．1999，11(4)：220～221
62．王付欣，谢德意，王惠萍，冯复全，张明勤 干旱胁迫对短季棉生理生化特性的影响，棉花学报，2001，28(11)：16—17
63．王关林，方宏筠 植物基因工程原理与技术．大连：科学出版社，1998．1～20，195，614～624
64．王国英，张宏，丁群星，戴景瑞，谢友菊 几种玉米基因转移技术的研究及转基因植株的获得．生物工程学报．1996，12(1)：45～49
65．王劲 烟草脱外壁花粉实验系统的建立及其遗传转化的研究：[博士学位论文]．武汉：武汉大学，1998
66．王宁宁，王勇，王淑芳，朱亮基，张韧6-BA延缓大豆叶片衰老的作用与膜蛋白磷酸化状态的关系．植物生理学报．1998，305～308
67．王三根，何立人，李正玮，曾金国，柴友荣，侯磊 淹水对大麦与小麦若干生理生化特性影响的比较研究．作物学报．1996，22(2)：228～232
68．王善平，许智宏，卫专明 毛白杨叶外植体的遗传转化．植物学报．1990，32(3)：172～177
69．王玉琴，林振武，吴少伯，赵毓桔，汤玉玮 6-苄氨基嘌呤促进黄化小麦叶片叶绿体的发育．植物生理学报．1982，8：45～52
70．王爱国，罗广华，邵从本，吴淑君，郭俊彦 大豆种子超氧化物歧化酶的研究．植物生理学报，1983，9(1)：77
71．王冀川，李志军，徐雅丽，于军，赵素珍，段黄金，黄琪 低温胁迫对海岛棉幼苗生理生化特性的影响，棉花学报，2001，28(5)：13—14
72．王伟，朱祯，邓朝阳，高越峰，徐鸿林，肖桂芳，郭仲琛，李向辉 陆地棉栽培品种新陆早1号转基因抗虫植株的获得，高技术通讯，1998，5：1～5
73．王伟，朱祯，高越峰，石春林，陈宛新，郭仲琛，李向辉 双价抗虫基因陆地棉转化植株的获得，植物学报，1999，41(4)：384～388
74．王喆之，李克勤，张大力，郝联芳，郭德志，刘全宏，肖娅萍，张苏锋 陆地棉胚性愈伤的组织的变异及高频胚胎发生。植物学报，1994，36(5)：331—338
75．王志斌，郭三堆 表达cryIA/gna双价抗虫基因烟草兼抗棉铃虫和蚜虫．科学通报，1999，44(19)：2068～2074
76．吴敬音，朱卫民，余建明，蔡小宁，朱祯，李向辉 陆地棉(G.hirsutum L.)茎尖分生组织培养及其在基因导入上的应用．棉花学报．1994，6(2)：89～92
77．吴霞，焦改丽，吴家和，范小平，孟晋红，罗小丽，上官小霞，李燕娥 棉花农杆菌介导法转化机理及研究进展，山西农业科学，2001，29(1)：27～30
78．夏英武，吴殿星，舒庆尧 农杆菌T—DNA介导的植物转基因的分子机制，生物学杂志，1994，5：7～12
79．向文胜，肖振平，赵长山 抗除草剂转基因作物．东北农业大学学报，1998，29(2)：201～208
80．肖凯，张荣铣外界条件对作物叶片衰老的影响．国外农学—麦类作物．1994，(3)：41～44
81．谢道昕，范云六，倪万潮，黄骏麒 苏云金芽孢杆菌杀虫晶体蛋白基因导入棉花获得转基因植物．中国科学(B辑)．1991，21(4)：367～373
82．徐明良，杨金水，葛扣麟 植物转基因的整合机制．植物生理学通讯，1996，32(3)：697～704
83．徐秋华 用RAPD标记分析我国陆地棉品种资源的遗传多样性：[硕士论文]．武汉：华中农业大学，2000
84．许耀，施骏，李宝健单、双子叶植物的代谢物调节农杆菌Vir区基因表达的研究，遗传学报，1993，20(1)：59～67
85．阎隆飞，刘国琴，肖兴国 从花粉肌动蛋白到作物雄性不育．科学通报，1999，44(23)：2471～2475
86．于晓红 朱勇清 种子特异表达ipt转基因棉花根和纤维的改变．植物学报．2000，42(2)：59～63
87．于元杰，尹承佾，沈法福，杨掀同，程立 应用植物分子育种技术选育棉花种质系．见：麦棉分子育种研究，成都：四川科学技术出版社，1995．第6～13页
88．喻树迅，范术丽，原日红，余学科，巩万奎 清除活性氧酶类对棉花早熟不早衰特性的遗
传影响，棉花学报，1999，11(2)：100—105
89．袁朝兴，丁静 水分胁迫对棉花叶片中IAA含量、IAA氧化酶和过氧化物酶活性的影响．植物生理学报．1990，2：179～184
90．岳建雄，石跃进，张慧军，王福社棉花试管苗“出管”移栽经验点滴．植物生理学通讯．1999，35(6)：482
91．张国栋，赵长山大豆抗除草剂特性的遗传及其利用．大豆科学，1991，10(4)：310～315
92．张家明，孙雪飘，郑学勤，张献龙，赵燕，刘金兰，孙济中 陆地棉愈伤诱导及胚胎发生能力的遗传分析，中国农业科学，1997，30(3)：36～43
93．张家明，孙济中，刘金兰，张献龙 陆地棉体细胞植株再生及其移栽技术研究．作物学报．1994，20(2)：210～215
94．张赛群 异戊烯基转移酶基因转化番茄及转基因植株再生：[硕士论文]．武汉：华中农业大学，1998
95．张献龙，孙济中，刘金兰 陆地棉体细胞胚胎发生与植株再生．遗传学报．1991，18(5)：461～467
96．张献龙，林双龙，吕复兵，董新国 陆地棉茎尖培养影响因素的研究．华中农业大学学报，1996，15(3)：210～214
97．张献龙，张家明，姚明镜，孙济中，刘金兰 棉花体细胞培养及其应用基础研究．华中农业大学学报，1993，12(5)：421—426
98．张宪政，陈凤玉，王荣富 主编．植物生理学实验技术．辽宁科学技术出版社．1994，66～69，184～186
99．郑泗军，吴吉祥，洪彩霞 棉花组织培养中畸形胚产生原因的分析。浙江农业大学学报，1993，19(2)：193—197
100．赵俊侠，焦改丽，李淑君，陈志贤 不同农杆菌菌株和棉花品种在转基因棉花中的应用研究．棉花学报．1997，9(4)：213～216
101．赵俊侠，齐宏力，焦改丽，陈志贤，南殿杰，杨苏龙，史俊东 棉花外源基因导入及转基因再生植株条件的研究．棉花学报．1998，10(4)：189～192
102．周光宇，龚蓁蓁，王自芬1979．远缘杂交的分子基础——DNA片段杂交假设的一个论证．遗传学报，6(4)：405～412
103．周光宇 分子育种—授粉后外源DNA导入植物技术，中国农业科学，1988，21(3)：1～6
104．周光宇 植物分子育种的兴起与展望，农业分子育种研究进展，中国农业科技出版社，1993：11～12
105．周岩，田颖川，吴标，莽克强 转雪花莲外源凝集素基因烟草对桃蚜的抑制作用．生物工
程学报，1998，14(1)：13～19
106．朱广廉，钟诲文，张爱琴编 小麦叶片在哀老过程中氧化脂质含量的变化．植物生理学实验．北京大学出版社．1990，245～248
107．朱卫民，吴敬音，佘建明，蔡小宁，朱祯，李向辉 棉花茎尖分生组织在微粒轰击法基因转化中的应用，江苏农业学报，1998，14(2)：74～79
108. A. Peri H. Kless,A. Blumenthal, G. Galili, and Galun. Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization of culture conditions and DNA-microprojectile delivery procedures, Mol Gen Genet, 1992, 235:279～284
109. Adang M J, Application of a Bacillus thuringiensis crystal protein for insect control. Molecular Strategies for Crop Protection, 1987, 345～353
110. Akama K, Puchta H, Hohn B Efficient Agrobacterium-mediated transformation of Arabidopsis thaliana using bar gene as selectable marker. Plant Cell Report, 1995,14; 450～454
111. Alejandro P V Expression of the hygromjcin B phosphotrasferase gene confers tolerance to the herbicide glyphosate. Plant Cell Report, 1995, 14: 482～487
112. Alfrid Nobel Drive, Hercukes, California Biolistic PDS-1000/He Particle Delivery System Instruction Manual. Bio Rad Laboratories, 94547 U.S.A. 1995
113. Allen R D, Trolinder NL Expression of superoxide dismutase in transgenic plants leads to increased stress tolerance. In: 1995 Beltwide Cotton conferences. [s.l.]:[s.n.], 1995.1136-1137
114. Altabellat T, Chrispeels M J Tobacco plants transformation with the bean α-ai gene express an inhibitor of insect α-amylase in their seeds. Plant Physiol, 1990,93:805～810
115. Barbieri L, Battelli M G, Stirpe F Ribosome-inactivating proteins from plants. Biochim. Biophys. Acta. 1993, 11 54:273～282
116. Batchvarova, V. Nikolaeva, S. Slavov, S. Bossolova, V. Valkov, S. Atanassova, S. Guelemerov, A. Atanassov, H. Anzai Transgenic tobacco cultivars resistant to Pseudomonas syringae pv. tabaci. Theor. Appl. Genet, 1998, 97:986～989
117. Bate NJ, Rothstein S J, Thorngson JE Expression of nuclear and chloroplast photosynthesis-specific genes during leave senescence. J Exp Bot,1991,42:801～811
118. Bayley, C, N. Trolinder, C. Ray, M. Morgan, J. E. Quisenberry, and D. W. Ow Engineering 2,4-D resistance into cotton. Theor. Appl. Genet. 1992,83:645～649
119. Beasly, C A In vitro culture of fertilized cotton ovules. Bioscience, 1971,21:906-907
120. Benedict J H, Ring D R,Sachs E S Influence of transgenic Bt cottons on tobacco budworm behavior, surival and plant injure.proceedings of cotton Beltiwide conferences,1992,599～601
121. Benfey P N, Chua N H Regulated gene in transgenic plants. Science, 1989, 244:174～181
122. Bouamama Efficiency of coupling biolistic and Agrobacterium in genetic transformation of tunisian autochthonous grapes, Agr. Med. Vol.2000, 130:223～227
123. Bradley D. The insecticidal Cry IB crystal protein of Bacillus thuringiensis subs. Has dual specificity to coleopteran and lepidopteran larvae. In: Abstracts of the ⅩⅩⅤ Annual meeting of the Society for Invertabrate pathology. Heidellbery, 1992, 234
124. Broer, I Stress inactivation of foreign genes in transgenic plants. Field Crop Research, 1996, 45(3),19～25
125. Buchanan-Wollaston V, Ainsworth C Leaf senescence in Brassica napus: cloning of senescence-related genes by subtractive hybridation. Plant Mol Biol, 1997,33:821～834
126. Buchanan-Wollaston V The molecular of leaf senescence. J Exp Bot, 1997,48:181～199
127. C. Zapata. S. H. Park. K. M. El-Zik. R. H. Smith Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex. Theor Appl Genet. 1999,98:252～256
128. Cao J, Duan X L, EcElroy, Cao, I., X. Duan, D. McElroy and R. Wu Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Reports, 1992, 11:586～591
129. Caryl A. Chlan, Junmin Lin, Jeffery W. Cary and Thomas E. Cleveland. A procedure for biolistic transformation and regeneration of transgenic cotton from meristematic tissue, Plant Molecular Report, 1995, 13(1): 31～37
130. Christou P, Ford TL, Kofron M Production of transgenic rice (Oryza sativa L.) plants from agronomically important Indica and Japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/technology, 1991,9:957～962
131. Clouse SD Molecular genetic studies confirm the role of brassinosteroids in plant growth and development. Plant J, 1996,10:1～8
132. Cook D, Rasche M, Eisnger W Regulation of ethylene biosynthesis and action in cut
carnation flower senescence by cytokinins. J Amer Soc Hort Sci, 1985, 110:24～27
133. Cousins Transformation of an Ausralian Cotton Cultivar: Prospects for Cotton Improvement through Genetic Engineering, Aust. J. Plant Physiol. 1991,18,481～494
134. Curtis I, He S, Scott C P, J Brian Power, Michael R Devey. Genomic male sterility in lettuce, a baseline for the production of F1 hybrids. Plant Sci, 1996, 113:113～119
135. D.C. Agrawal, A. K. Banerjee, R. R. Kolala, A. B.Dhage, A. V. Kulkarni, S. M. Nalawade, S. Hazra, K. V. Krishnamurthy, In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.), Plant Cell Rep, 1997, 16: 647652
136. Davidonis, G H and Hamilton, R H. Plant regeneration from callus tissues of Gossypium hirsutum L. Plant Scei. Lett, 1983,32:89～93
137. Davies KM, Grierson D Identification of cDNA clones for tomato (Lycopersicon esculenturn Mill.) mRNAs that accumulate during fruit ripening and leaf senescence in response to ethylene. Planta, 1989,179:73～80
138. Davies W J, Jianhua Z Root signals and the regulation of growth and development of plants in drying soil. Ann Rev Plant Physiol plant Mol Biol, 1991,42:55
139. Delong A, Calderon-Urrea A, Dellaporta SL Sex determination gene TASSELSEED2 of maize encodes a short-chain alcohol dehydrogenade required for stage-specific floral ofgan abortion. Cell,1993,74:757～768
140. Denis M, Delourme R, Gourret J P, Mariani C, Renard M Expression of engineered nuclear male sterility in Brassica napus. Plant Physiol, 1993, 107:1295～1304
141. Dennis Bidney, Chris Scelonge, Joanie Martich, Monique Burrus, Lynn Sims and Gary Huffiman, Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens, Plant Molecular Biology, 1992,18:301～313
142. Down R E, Gatehouse A M R, Hamilton W D O, et al Snowdrop lectin inhibits development and decreases fecundity of glasshouse potato aphid (Aulacorthum solani) when administered in vitro and via transgenic plants both in laboratory and glasshouse trails. J. Insect Physiol, 1996, 42:1035～1045
143. Downes B P, Crowell D N Cytokinin regulates the expression of a soybean β-expansin gene by a post-transcriptional mechanism. Plant Mol Biol, 1998,37:437～444
144. Ebrahim Firoozabably David L, DeBoer, Donald J. Merlo, Edward L. Halk, Lorraine N. Amerson, Kay E. Rashka and Elizabeth E. Murray. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants, Plant Molecular Biology, 1987, 10: 105～116
145. Eliseu S, Figucrira F, Lucio F A Transformation of pototo (Solanum fuberosum) cv. Mantigueira using Agrobacterium tumefaciens and evaluation of herbicide resistance. Plant Cell Report, 1994, 13:666～670
146. Firoozabady, E., D.L. DeBoer, D.J. Merlo, E.L. Halk, L.N. Amerson, K.E. Rashka, and E.E. Murray Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants. Plant. Mol. Biol. 1987,10:105～116
147. Frederick J. Perlak, Randy W. Deaton, Toni A. Armstrong, Roy L. Fuchs, Steven R. Sims, John T. Greenplate and David A. Fischhoff. Insect resistant cotton plants, Bio/Technology, 1990,8:939-942.
148. Fullner K J, Lara J C, Nester E W Pilus assembly by Agrobacterium T-DNA transfer genes. Science, 1996,273:1107～1109
149. Gan S, Amasino RM Inhibition of leaf senescence by autoregulated production of cytokinin.Sci. 1995, 270:1986～1988
150. Gan S, Amasino RM Making senescence: molecular gentic regulation and manipulation of leaf senescince. Plant Physiol, 1997,113:313～319
151. Ganesan Sunilkumar and Keerti S. Rathore. Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration, Molecular Breeding, 2001,8:37～52
152. Gasser C S, Fraiey R T Genetically engineering plants for crop improvement. Science, 1989, 244:1293～1299
153. Genevieve Hansen Constitutive expression of the virulence gene improve the efficiency of plant transformation by Agrobacterium, Plant Biology, 1994, 91: 7603～7607
154. Genevieve Hansen and Mary-Dell Chilton. "Agrolistic" transformation of plant cells integration of T-strands generated in planta. Proc Natl Acad Sci USA, 1996,93:14978-14983
155. Georage C. Allen, Gerald Hall, Jr., Susan Michalowski, Winnell Newman, Steven Spiker, Arthur K. Weissinger, and William F. Thompson, High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco, Plant Cell, 1996, 8(5), 899～913
156. Ghosh B, Biswas G C, Lglesias V A, Datta S K, Potrykus L Transgenic indica rice plants obtained by direct gene transfer to protoplasts, J.BIOTECH, 1994, 32:1～10
157. Gosset DR et al Antioxidant status in salt stressed cotton. Beltwide Cotton Conferences of America 1992, 1036-1
158. Grabam IA, Leaver CJ, Smith SM. Induction of malate wynthas gene expression in senescent and detached organs of cucumber. Plant Cell, 1992, 4:349～357
159. Grbic V, Bleecker AB Ethylene regulates the timing of leaf senescence in Arabidopsis. Plant J, 1995, 8:595～692
160. Greg Keller, Lori Spatola, Demis Mccabe, Brian Martinell, William, Swain, Maliyakal E. John. Transgenic cotton resistant to herbicide bialaphos,Transgenic Research, 1997,6:385～392
161. Gupta S K, Alok K. Sdvastava, Pradhyumna K. Singh and Rakesh Tuli. In vitro proliferation of shoots and regeneration of cotton, Plant Cell Tiss Organ Cul, 1997, 51: 149～152
162. HaKe S Unraveling the knots in plant development. Trends in Genetics.1992,8:109～114
163. Hain R, Reif H J, Krause E, Langebartels R, Kindl H, Vornam B, Wiese W, Schmelzer E,Schreier P H, Stocker R H,Stenzel K. Disease resistance results from foreige phytoalexin expression in a novel plant. Nature, 1993, 361:153～156
164. Hamilton A J, Lycett GW, Grierson D.Antisense gene that inhibbits synthesis of the hormonce ethylene in transgenic plants. Nature, 1990,346:284～287
165. Hanfrey C, Fife M, Buchanan-Wollaston V Leaf senescence in Brassica napas: expression of genes encoding pathogenesis-related proteins. Plant Mol Biol. 1996,30: 597～609
166. Hensel LL, Grbic V, Baumgarten DA and Anthony B. Bleecker. Developmental and age-related process that influence the longevity and senescence of photosynthetic tissues in Arabidopsis Plant Cell, 1993,5:553～564
167. Hooykaas P J J, Schilperoort R A Agrobacterium and plant genetic engineering. Plant Mol Biol, 1992,19:15～38
168. Hooykaas P J J, Beijersbergen A G M The virulence system of Agrobacterium tumefaciens. Annu Rev Phytopathol, 1994, 32:157～179
169. Horsh R B, Fry J E, Hoffmann N L, Eichholtz. D, Rogers. S G, Fraleg R T. A simple and general method for transferring genes into plants. Scince, 1985, 227:1229～1231
170. Hradecka D, Staszkova L Influence of the application of cytokinin and nitrogen fertilizer on spring wheat. Rostlinna Vyroba, 1996,42(7): 301～306
171. Huang Y, Nordeen R O, Di M, L. D. Owens, J.H. Mcbeath. Expression of an engineered
Cecropin gene cassette in transgenic tobacco plants confers disease resistance to Pseudomonas syringae pv. tabaci. Phytopathology, 1997, 87:494～499
172. Isaac Jone, Rfarrell Drake, Aldo Farrel, Wendy Cooper, Pam Lee, Peter Horton and Don Grierson. Delayed leaf senescence in ethylene-deficient ACC oxidase antisense tomato plants: molecuar and physiolo-gical analysis. Plant J, 1995,7:483～490
173. Ivanova A P Stefanov K L, Yordanov I T Effect of cytokinin and cytokinin 4PU-30 on the lipik composition of water stressed bean plants. Biologia Plantarum.1998, 41(1): 155～156
174. J.K. Hemphill C. G. A. Maier K. D. Chapman. Rapid in vitro plant regeneration of cotton (Gossypium hirsutum L.), Plant Cell Rep, 1998, 17:273～278
175. J. Ke R. Kham T. Johnson D. A. Somers A. Das. High-efficiency gene transfer to recalcitrant plants by Agrobacterium tumfaciens, Plant Cell Reports,2001,20:150～156
176. Jach G, Gornhardt B, Mundy J, Guido Jach, Birgit Grnhard, John Mundy, Jürgen Logemann, Elke Pinsdorf, Robert Leah, Jeff Schell and Christoph Maas Enhanced quantitative resistance against fungal disease by combinatoril expression of defferent barley antifungal proteins in transgenic tobacco. Plant J, 1995, 8:97～109
177. Jefferey W. Pollard and John M. Walker Plant Cell and Tissue Culture: Meth-ods in Molecular Biology.6. Hummana Press. Clifton, New Jersey
178. Jean Gould, Sharon Banister, Osamu Hasegawa, Michal Fahima, Roberta H.Smith. Regeneration of Gossypium hirsutum and G. barbadense from shoot apex tissues for transformation, Plant Cell Rep, 1991, 10:12～16
179. Jiang CZ, Rodermel SR, Shibles RM Photosynthesis, rubisco activity and amount, and their regulation by transcription in sdndscing soybean leaves. Plant Physiol, 1993,101:105～112
180. John J. Finer, Transformation of cotton (Gossypium hirsutum L.) via particle bombardment, Plant Cell Reports, 1990, 8:586～589
181. Jongedijk E, Tigelaar H, van Roekel J S C, Sandra A Bres_Vloemans, llma Dekker, Peter J. M, Van den Elzen, Ben J. C. Comelissen, Leo S. Melchers. Synergistic activity of chitinases and β-1,3-glucanases enhances fungal resistance in transgenic tomato plants. Euphytica, 1995, 85:173～180
182. Kawami N and W Keller. Transgenic cotton resistant to herbicide bialaphos, Transgenic Research, 1997,6:385～392atanabe A Change in gene expression in radish cotyledons during dark-induced senescence. Plant Cell Physiol.1988a,29:33～42
183. Kim DJ, Smith SM Molecular cloning of cucumber PEP carboxykinase and developmental regulation of gene expression. Plant Mol Biol.1994a,26:423～434
184. Kim DJ, Smith SM Expression of a single gene encoding microbody NAD-malate Dehydrogenade during glyoxysome and peroxisome development in cucumber. Plant Mol Biol. 1994b, 26:1833～1841
185. K. Rajasekaran High-frequency stable transformation of cotton (Gossypium hirsutum L.) by particle bombardment of embryogenic cell suspension cultures, Plant Cell Reports, 2000, 19: 539～545
186. Klein T. M, E. D. Wolft, R. Wu and C. Sanford. High-velocity microprojectile for delivery of mucleic acids into living cells, Nature, 1987, 327:70～73
187. Koizumi M, Yaneguchi-shinozaki K, Tsuji H et al Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana. Gene, 1993,129:175～182
188. Kramer C, DiMaio J, Carswell G K Selection of transformed protoplast-derived Zea mays colonies with phosphinothdcin and a novel assay using the PH indicator cholorophenol red. Planta, 1993, 190:454～458
189. Kranz E, Lorz H Micromanipulation and in vitro fertilization with single pollen grains of maize Sex Plant Reprod, 1990,3:160～169
190. Lane B G, Dunwell J M, Ray J A, Mark R. Schmitt, Andrew C. Cuming. Germin. A protein marker of early plant development, is an oxalate oxidase, J. Biol. Chem. 1993, 268(17): 12239～12242
191. Lee J H, Montagu M V, Verbruggen N A highly conserved kinase is an essential component for stress tolerance in yeast and plant cells. Proc. Natl. Acad. Sci. USA, 1999, 96: 5873～5877
192. Lee L Y, Gelvin S B, Kado CI pSa causes oncogenic suppression of Agrobacterium by inhibiting VirE2 protein export. J Bacteriol, 1999, 181(1): 186～196
193. Lejeune P, Prinsen E. Onckelen H V, Bernier C, Van O H Hormonal control of ear abortion in a stress-sensitive maize inbred. Australian J Plant Physiol, 1998,25(4): 481～488
194. Li fuguang, Study on Insect-resistant Transgenic Cotton Harbouring Double-gene and Its resistance to Insect Pests, Chinese Agricultural Sciences, 2001, No.1, 70～76
195. Lin, W., Anuratha, C. S., Datta, K., Potrykus, I., Muthukrishnan, S. and Datta, S. K Genetic engineering of rice for resistance to sheath bilght. Bio/Technology,
1995,13:686～691
196. Linda L. Hensel, Vojislava Grbic, David A. Baumgarten, Anthony B. Bleeker, Developmental and age-related process that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell, 1993,5:553～564
197. Lohman KN, Gan S, John MC and Amasino. R. M. Molecular analysis of natural leaf senescene in Arabidopsis thaliana. Physiol Planta, 1994, 92: 322～328
198. Mariani C, Gossele V, Beuckeleer M D, et al A chimaeric ribonuclease inhibitor gene restores fertility to male sterile plants. Nature, 1992, 357:384～387
199. Matthews BF, Abdul-Baki AA, Saunders JA Expression of a foreign gene in electropotated pollen grains of tobacco. Sex Plant Reprod, 1990,3:147～151
200. Mayerhofer R, Konz-Kalman Z, Nawrath C, Bakkeren G, Crameri A, Angelis K, Redei GP, Schell J, Hohn B, and Koncz C T-DNA intrgration: amode of illegitimate recombination in plants. The EMBO J, 1991, 10(3): 697～704
201. Mazur B J, Carl F S The development of herbicide-resistant crops. Annu Plant Physiol & Plant Mol. Biol, 1989, 40:441～470
202. McCabe DE, Christou P Direct DNA transfer using electric discharge particle acceleration technology. Plant cell and Organ Culture, 1993,33:227～236
203. McCabe D. E. and Martinell B.J. Transformation of elite cotton cultivar via particle bombardment of meristems, Bio-technology, 1993, 11(5): 596～598
204. Michael J. Adang, Michael J. Staver, Thomas A. Rocheleau, Jacqui Leighton, Richard F. Barker and David V. Thompso, Staver M J et al Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subs. Kurstaki HD-73 and their toxicity to Manduca Sexta. Gene, 1985, 36 289～300
205. Morre J L, Hugo R. Permingeat, Maria V. Romagnoli, Cinitia M. Heisterborg and Ruben H. Vallejos Multiple shoot induction and plant regeneration from embryonic axes of cotton, Plant Cell Tiss Organ Cult, 1998, 54:131～136
206. Murray F R, Lewellyn D J, Peacock W J Isolation of the glucose oxidase gene form Talaromyces flavus and charactrisation of ite role in the biocontrol of Verticillium dahliae. Current Genetics, 1997, 32:367～375
207. Nakamura Y, Sawada H, obayashi S, Nakajima I, Yoshikawa M Expression of soybean β-1,3-endogiucanase cDNA and effect on disease tolerance in kiwifruit plants. Plant Cell Report, 1999,18:527～532
208. P. Venkatachalam N, Geetha, Abha Khandelwal, M. S. Shaila and G. Lakshmi Sita. Agtobacteium-mediated genetic transformation and regeneration of transgenic plants from cotyledon explants of groundnut (Arachis Hypogaeal.) via somatic embrygenesis, Current Science, 2000, 78 (9): 1130～1136
209. Paterson A H, Brubaker C L, Wendel J G Arapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis. Plant Mol Biol Rep, 1993,11:122～127
210. Paul Umbeck, Gail Johnson, Ken Barton, and Will Swain. Genetically transformed cotton (Gossypium hirsutum L.) plants, Bio-technology, 1987, 5:263～266
211. Paula Elomaa Yrjo Helariutta. Robert J. Griesbach Mika Kotilainen. Pauli Seppanen. Teemu H. Teeri. transgene inactivation in Petunia hybrida is influenced by the properties of the foreign gene. Molecular and General Genetics, 1995, 248, 649～656
212. Philip N. Benfey and Nam-Hai Chua, Regulated Genes in Transgenic Plant. Science, 1989, 224, 174～181
213. Powell-Abel, P.P, Nelson, R.S, De, B, Hoffmann, N, Rogers, S.G, Fraley, R.T, and Beachy, R.N Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science1986, 232:738-743
214. Price, H J and smith, R. H Somatic embryogenesis in suspension culture of Gossypium Klotzschiarum Anderss. Plant, 1979,305～307
215. Purnhauser L. Medgyesy P, Czako M Philio J. Dix and Laszlo Marton. Stimulation of shoot regeneration in Triticum aestivum and Nicotiana Plumbaguifolia Viiv. tissue culture uding the ethylene inhibitor AgNO3. Plant Cell Rep. 1987, 6:1～4
216. Simon C. Deroles and Richard C. Gardner Analysis of the T-DNA structure in a large number of transgenic petunias generated by agrobaccterium-mediated transformation. Plant Mol. Biol. 1988, 11,365～377
217. R.B. Horsch J. E. Fry N.L.Hoffmann D. Eichholtz S.G.Rogers R.T. Fraley. A simple and general method for transferring genes into plants, Science, 1985,217:1229～1231
218. Reynolds D B, Crawford S H, Rogers R L Weed control in transgenic Buctril herbicide-tolerant cotton. Louisiana Agri, 1994, 37(3): 13～14
219. Rupp H. M Cotykinin overproduction in transgenic Arabidopsis thaliana. The 8th Int'l. Cong. on Arabidopsis thaliana Rech. Wisconsin, July 1997
220. Ryu SB, Wang XM Expression of phospholipase-D during castor bean leaf senescene. Plant Physiol.1995,108:713～719
221. S.H. Park, Short T-DNA or additional virulence genes improve Agrobacterium--mediated transformation, Theor Appl Genet, 2000, 101:1015～1020
222. Saeed N A, Yusuf Zafar and Kauser A. Malik. A simple procedure of Gossipium meristem shoot tip culture, Plant Cell Tiss Organ Cul, 1997, 51:201～207
223. Sanford J.C Deliver of substance into cells and tissues using aparticle bombardment, Process J Part Sci Technol, 1987, 5:27～37
224. Schuler T H, Poppy G M Insect-resistant transgenic plants. Tibtech, 1998,16:168～175
225. Smart C. M, Hosken S. E, Thomas H. John A. Greavea, Bruce G, Blair and Wolfgang Schuch.The timing of maize leaf senescence and characterization of senescence-related cDNAs. Physiol Plant, 1995,93:673～682
226. Smigocki. A., Neal. J. W. Jr., McCanna, I. and Douglass, L Cytokinin-mediated insect resistance in Nicotiana plants transformed with the ipt gene. Plant Molecular Biology. 1993, 23:325-335.
227. Smith CR, Saunders JA Expression of GUS and CAT activities using electransformed pollen. Plant Sci 1994,104:49～58
228. Song W Y, Wang G L, Ronald P, Wen-Yuan Song, Guo-Liang Wang, Li-Li Chen, Han-Suk Kim, Li-Ya Pi, Tom Holsten, J. Gardner, Bei Wang, Wen-Xue Zhai, Li-Huang Zhu, Claude Fauquet, and Pamela Ronald Areceptor kinase-like protein encoded by rice disease resistance gene, Xa21. Science, 1995, 270:1804～1806
229. Songstad. D, Duncan. DR, Wildholm. J, M Effect of 1-aminocyclopropane 2-carboxylic cid. silver nitrate.and norbornadiene on plant regeneration from maize callus cultures. Plant Cell Rep. 1988,7:262～265
230. Stoger E, William S, Christou P Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid sitobion avenae. Molecular Breeding, 1999, 5(1):65～73
231. Strauss S H, Rottmann W H, Brunner A M, Lorraine A Genetic engineering of reproductive sterility in forest trees. Molecular Breeding, 1995, 1:5～26
232. Sundberg, C., L. Meek, K. Carroll, A. Das and W. Ream VirE1 protein mediates export of the single-stranded DNA-binding protein VirE2 from Agrobacterium tumefaciens into plant cells. J Bacteriol, 1996, 178(4): 1207～1212
233. Tabei Y, Kitade S, Nishizawa Y, Kikuchi N, Eayano T, Hibi T, Akutsu K Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea). Plant Cell Reports, 1998, 17:159～164
234. Takaichi M, Oeda K Transgenic carrots with enhanced resistance against two major pathogens, Etysiphe heraclei and Alternaria dauci. Plant Science, 2000, 153:135～144
235. Taylor CB, Bariola PA, Delcardare SB Ronald T. Raines, Pamela J. Green. RNS2: a senescence-associated Rnase of Arobidopsis that diverged from the S-Rnases before speciation. Proc Natl Acad Sci USA. 1993,90:5118～5122
236. Thea A. Wilkins Kanniah Rajasekaran and David M. Anderson. Cotton biotechnology, Critical Reviens in Plant Sciences, 2000, 19(6): 511～550
237. Thomas H. Sid a Mendelian locus controlling thylakoid membrane disassemhlyin senescencing leaves of Festuca pratensis. Theoretical and Applied Genetics. 1987,73:551～555
238. Thompson C, Dunwell J M, Johnstone C E, Lay V, Ray J, Schmitt M, Watson H, Nisbet G Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase. Euphytica, 1995, 85:169～172
239. Thomzik, JE, Stenzel, K., Stocker, R., Schreier, PH, Hain, R., and Stahl, DJ Synthesis of a grapevine phytoalexin in transgenic tomatoes (Lycopersicon esculentum) conditions resistance against phytophthora infestns. Physiol Nol. Plant Pathol, 1997,51:265～278
240. Touraew A, Stoger E, Voronin V, Hebede-Bors E Plant male germ line transformation. Plant J, 1997,12:949～956
241. Trick H N, Finer J J. SAAT: soniation-assisted Agrobaterium-mediated transformation. Transgenic Researh, 1997, 6:329～336
242. Trolinder, N L Somatic embryogenesis in Cell suspension cultures of G.hirsutum. procedlings Beltwide cotton production research conference. 1984,46
243. Trolinder N Land J. R. Goodin. Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.), Plant Cell Rep, 1988, 6:231～234
244. Twell D, Klein T, Fromm ME, McCormick S Transient expression of chimeric genes dilivered into pollen by microprojectile bombardment. Plant Physiol, 1989:1270～1274
245. Umbeck, P., G. Johnson, K. Barton, and W. Swain Genetically transformed cotton (Gossypium hirsutum L.) plants. Bio/Technology. 1987,5:263～266
246. Vermenlen A, Vaucheret H Agrobacterium mediated transfer of a mutant Arabidopsis acetolacte synthase gene confers resistance to chlorsulfuron in chicory. Plant Cell Report, 1992, 11:243～247
247. W. Cherdshewasart, G.B. Ghareti-chhetri, M. W. Saul, M. Jacobs and I. Negrutiu. Expression instability and genetic disorders in transgenic Nicotiana plumbaginifolia plants. Transgenic Research, 1993 2(6), 307～320
248. Watanabe H, Abe K, Emori Yhosoyama, and Soichi Ami. Molecular cloning and giberellion-induced expression of multople cysteine proteinases of rice seeds (oryzains). J Biol Chem. 1991,266:16897～16902
249. Willy Dillen, Janniek De Clercq, Jyoti Kapila, Mukund Zambre, Marc Van Montagu and Geert Angenon. The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer to plants, The Plant Journal, 1997,12(6):1459～1463
250. Wu G, Shortt B J, Lawrence E B, Levine B, Fitzsimmons K. C, and Shah D. M Disease resistance conferred by expression of a gene encoding H2O2-generating glucose potato plants. Plant Cell, 1995, 7:1357～1368
251. Xia G M, Li Z Y, He C X, Chen HM, Ricard Brettell. Transgenic plant regeneration from wheat (Triticum aestivum L.) mediated by Agrobacterium tumefaciens. 植物生理学报，1999, 25(1):22～28
252. Yi-chang wang Theodore M. Klein, Michael Fromm, Jun Cao, John C. Sanford and Ray Wu. Transient expression of foreign gene in rice, wheat and soybean cell following particle bombardment, Plant Molecular Biology, 1988, 11:433～439
253. Zambryske P Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regulation apacity. EMBO J., 1983, 2:2143～2150
254. Zambryski P C Chronicles from the Agrobacterium-plant cell DNA transfer story. Annu Rev Plant Physiol Plant Mol Biol, 1992, 43:465～490
255. Zhou H, Arrowsmith J.W, Fromm M.E, Hironaka C.M, Taylor M.L, Rodriguez D, Pajeay M.E, Brown S.M, Santino C.G, and Fry J.E Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Report, 1995, 15:159～163
256. Zou J T, Zhan X Y, Wu H M, et al Characterization of a rice pollen-specific gene and its expression. Am J Bot, 1994,81:552～561
257. Zupan J R, Zambryski P C Transfer of T-DNA from Agrobacterium to the plant cell. Plant Physiol, 1995,171:1041～1047
2．陈奇思，田明军，吴云康棉花生育规律与优质高产高效栽培．中国农业出版社，1997
3．陈思学 农杆菌与植物细胞的相互作用，生物学通报，1993，28(3)：13
4．陈思学，黄祥辉 农杆菌与植物细胞识别研究的进展，生物学杂志，1995，5：12～14
5．陈志贤，N.L.Trolinder, J.R.Goodin棉花细胞悬浮培养胚胎发生和植株再生某些特性的研究，中国农业科学．1987，20(5)：6～11
6．陈志贤，Danny J．Llewellyn，范云六，李淑君，郭三堆，焦改丽，赵俊侠 利用农杆菌介导法转移tfdA基因获得可遗传的抗2，4—D棉株。中国农业科学．1994，27(2)：31～37
7．崔洪志，郭三堆，Bt毒蛋白抗虫植物基因工程研究，农业生物技术学报，1998，6(2)：166～172
8．丁群星，谢友菊，戴景瑞，米景九，李太元，田颖川，乔利亚，莽克强，刘宝兰，王音，冯平章 用子房注射法将Bt毒蛋白基因导入玉米的研究．中国科学(B辑)．1993，23(7)：707～713
9．董合忠，焦改丽2，4—D、KT对棉花愈伤组织的诱导和体细胞胚胎发生的影响，华北农学报，1993，8(3)：87～91
10．段俊，梁承邺，张明永玉米细胞质雄性不育与膜脂过氧化的关系．植物生理学通讯．1996，32(5)：331-334
11．段留生，张一，何钟佩，翟志席 生长素和细胞分裂素对棉花幼苗根系顶端优势的调控作用，棉花学报，2001，13(6)：330-333
12．范月仙，张述义，李生泉 棉花苗期抗冷性与可溶性蛋白含量增加的研究，山西农业大学学报，1995，15(1)：56～58
13．范月仙，张述义 棉苗抗冷性与POD活性变化关系的研究，棉花学报，1995，7(3)：172～174
14．范云六，张春义，贾继增 我国农作物生物技术的成就与展望，世界科技研究与发展，1999，21(1)，11～15
15．傅荣昭，孙勇如，贾士荣 植物遗传转化技术手册．北京：中国科学技术出版社，1994．35～39
16．高丽锋，胡志昂，王红新，阳文龙 根癌菌结瘤因子的结构和功能，生命科学，2002，14(1)：17～19
17．何钟佩 主编．农作物化学控制实验指导．北京农业大学出版社，1990：60-68
18．何钟佩 主编．作物激素生理及化学控制．中国农业大学出版社．1997：226～230
19．侯彩霞，汤章城 细胞相容性物质的生理功能及其作用机制．植物生理学通讯．1999，35(1)
20．侯文胜，郭三堆，路明 利用转基因技术进行植物遗传改良．生物技术通报．2002，1：10～15
21．胡延章，徐云剑，江国英，朱玉贤 转Bank I T4基因烟草的抗虫性，植物学报，2000，42(7)：732～735
22．耿飒，麻密 ipt基因定位表达对转基因烟草育种的影响．植物学报．2000，42(2)：217～220
23．巩万奎，喻树迅，陈志贤，吴家和，余学科 葡萄糖氧化酶基因在棉花中的高效转化．棉花学报．1999，11(5)：241～246
24．郭三维 植物BT抗虫基因工程研究进展．中国农业科学．1995，28(5)：8～13
25．郭三堆，崔洪志，夏兰芹，武东亮，倪万潮，张震林，张保龙，徐英俊 双价抗虫转基因棉花研究，中国农业科学，1999，32(3)：1～7
26．郝联芳，刘养浩 陆地棉基因转移技术研究初报，陕西师范大学学报(自然科学版)，1997，21(1)：83～86
27．郝秀英，李仁敬 用基因枪转化新疆陆地棉新陆早4号，新疆农业科学，1999，6：255～256
28．黄骏麒，钱思颖，刘桂玲，翁坚，曾以申，周光宇 外源海岛棉DNA导致陆地棉性状的变异．遗传学报，1981，8(1)：56～62
29．贾士荣，郭三堆，安道昌 棉花转基因，北京，科学出版社，2001
30．贾士荣 T-DNA转移机理，植物生理学通讯，1994，30(4)：306～311
31．焦改丽等，郭三堆，李淑君，陈志贤，赵俊侠 棉花遗传转化和植株再生的研究，华北农学报，1997，12(2)：82～85
32．李宝平，赵俊侠等 关键因子对棉花利用农杆菌介导法导入外源基因的影响．作物学报．2001，27(1)：80～84
33．李付广，周勇，刘传亮，李凤莲，李秀兰 影响陆地棉遗传转化的外部因子研究，西北植物学报，1999，19(3)：387—392
34．李国胜，刘玉乐，朱峰等基因工程雄性不育烟草的获得．植物学报，1995，37(8)：659～660
35．李洪清，李美茹 影响农杆菌介导植物基因转化的因素问题，植物生理学通讯，1999，35(2)：145～151
36．李克勤，王喆之，张大力，郭德志，郝联芳，张苏峰，沈志刚 陆地棉组织细胞培养的研究(J)。西北植物学报，1991，(11)：144—153
37．李卫，郭光沁，郑国锠 根癌农杆菌介导遗传转化研究的若干新进展，科学通报，2000，
45(8)：798～807
38．李晓，王学德，朱玉贤，卢迎春，赵向前 农杆菌真空渗透法转化花粉获得棉花转acsB基因植株，作物学报，2002，28(5)：709～712
39．李燕娥，朱祯，陈志贤，吴霞，王伟，李淑君，朱玉，焦改丽，吴家和，徐鸿林，范小平，孟晋红，肖桂芳，李向辉 豇豆胰蛋白酶抑制转基因棉花的获得．棉花学报．1998，10(5)：237～243
40．李燕娥，焦改丽，吴家和，石跃进，吴霞，范小平，孟晋红，李淑君，陈志贤，郭三堆，朱祯，田颖川 棉花农杆菌介导高效转化体系．中国棉花．2000，27(5)：10～11
41．李艳红，肖兴国，赵广荣，聂秀玲，张爱民，阎隆飞 将新的人工雄性不育基因导入小麦栽培品种的研究初报．农业生物技术学报，1999，7(3)：255～258
42．李子银，胡会庆 农杆菌介导的植物遗传转化进展，生物工程进展，1998，18(1)：22～26，16
43．李宗霆，周燮 植物激素及其免疫检测技术．南京：江苏科学技术出版社，1996
44．立洪清，李美茹 影响农杆菌介导植物基因转化的因素问题．植物生理学通讯，1999，35(2)145-151
45．廖祥儒，贺普超，朱新 玉米素对盐渍政葡萄叶圆片H_2O_2清除系统的影响．植物学报．1997，39(7)：641～646
46．刘春明，姚敦义 陆地棉体细胞胚发生及其细胞组织学研究，植物学报，1991，336(5)：378—384
47．刘志，袁小玲，张天真 获得多价转基因作物的策略，遗传，2001，23(2)：132～136
48．刘志学，马向前，何艺园，徐亚男，叶明鸣，唐克轩 农杆菌介导遗传转化中辅助处理方法的改良，复旦学报(自然科学版)，1999，38(5)：601～604
49．刘博林，岳绍先，胡乃璧，李小兵，翟文学，李诺，朱荣焕，朱立煌，毛大璋，周佩珍 龙葵Atrazine抗性基因向大豆叶绿体的转移及在转基因植株中的表达．中国科学B辑．1989，7：699～705
50．刘道宏 植物叶片的衰老．植物生理学通讯．1983，(2)：14～19
51．马庆虎，张韧，Higgins Thomas J V 异戊烯基转移酶基因在转基因烟草中的特异性表达．植物学报．1994，36(5)：339～344
52．毛树春，韩迎春，宋美珍，张朝军，范正义 中棉所29栽培技术及其生理特性研究棉花学报，2001，3(2)：82～86
53．明小天，苑华毅，陈章良 通过基因枪提高根癌农杆菌转化水稻的效率．植物学报．2001，43(1)：72～76
54．萨姆布鲁克J，弗里奇EF,曼尼阿蒂斯T 分子克隆实验指南，第二版．北京科技出版社．金冬雁，犁孟枫译，1992．19～34
55．苏金，J．Targolli，吴乃虎，吴瑞 在转基因植物中实现外源基因最佳表达的途径，生物工程进展，1999，19(4)：3～6
56．沈成国，张福锁，毛达如 植物叶片衰老过程中基因的表达与调控．植物生理学通讯．1998，34(4)：304～311
57．汤学军，王康 激动素和抗坏血酸保护受冷害功细胞膜和促进SOD合成的效应．植物学报(增刊)．1993，45～49
58．万丙良，张献龙 基因枪介导转化水稻花粉获得转基因植株．农业生物技术学报．2000，8(4)：332
59．魏伟，钱迎倩，马克平 转基因作物与其野生亲缘种间的基因流．植物学报1999，41(4)：343-348
60．王从丽，陆柏方，张学成，李广旭，王劲波，李延军，彭友良，潘申权 农杆菌—植物间基因转移的分子基础，生命科学，2002，14(1)：1～5
61．王长海，蓝海燕 利用花粉管通道法将外源质粒DNA注入棉花的改良方法．棉花学报．1999，11(4)：220～221
62．王付欣，谢德意，王惠萍，冯复全，张明勤 干旱胁迫对短季棉生理生化特性的影响，棉花学报，2001，28(11)：16—17
63．王关林，方宏筠 植物基因工程原理与技术．大连：科学出版社，1998．1～20，195，614～624
64．王国英，张宏，丁群星，戴景瑞，谢友菊 几种玉米基因转移技术的研究及转基因植株的获得．生物工程学报．1996，12(1)：45～49
65．王劲 烟草脱外壁花粉实验系统的建立及其遗传转化的研究：[博士学位论文]．武汉：武汉大学，1998
66．王宁宁，王勇，王淑芳，朱亮基，张韧6-BA延缓大豆叶片衰老的作用与膜蛋白磷酸化状态的关系．植物生理学报．1998，305～308
67．王三根，何立人，李正玮，曾金国，柴友荣，侯磊 淹水对大麦与小麦若干生理生化特性影响的比较研究．作物学报．1996，22(2)：228～232
68．王善平，许智宏，卫专明 毛白杨叶外植体的遗传转化．植物学报．1990，32(3)：172～177
69．王玉琴，林振武，吴少伯，赵毓桔，汤玉玮 6-苄氨基嘌呤促进黄化小麦叶片叶绿体的发育．植物生理学报．1982，8：45～52
70．王爱国，罗广华，邵从本，吴淑君，郭俊彦 大豆种子超氧化物歧化酶的研究．植物生理学报，1983，9(1)：77
71．王冀川，李志军，徐雅丽，于军，赵素珍，段黄金，黄琪 低温胁迫对海岛棉幼苗生理生化特性的影响，棉花学报，2001，28(5)：13—14
72．王伟，朱祯，邓朝阳，高越峰，徐鸿林，肖桂芳，郭仲琛，李向辉 陆地棉栽培品种新陆早1号转基因抗虫植株的获得，高技术通讯，1998，5：1～5
73．王伟，朱祯，高越峰，石春林，陈宛新，郭仲琛，李向辉 双价抗虫基因陆地棉转化植株的获得，植物学报，1999，41(4)：384～388
74．王喆之，李克勤，张大力，郝联芳，郭德志，刘全宏，肖娅萍，张苏锋 陆地棉胚性愈伤的组织的变异及高频胚胎发生。植物学报，1994，36(5)：331—338
75．王志斌，郭三堆 表达cryIA/gna双价抗虫基因烟草兼抗棉铃虫和蚜虫．科学通报，1999，44(19)：2068～2074
76．吴敬音，朱卫民，余建明，蔡小宁，朱祯，李向辉 陆地棉(G.hirsutum L.)茎尖分生组织培养及其在基因导入上的应用．棉花学报．1994，6(2)：89～92
77．吴霞，焦改丽，吴家和，范小平，孟晋红，罗小丽，上官小霞，李燕娥 棉花农杆菌介导法转化机理及研究进展，山西农业科学，2001，29(1)：27～30
78．夏英武，吴殿星，舒庆尧 农杆菌T—DNA介导的植物转基因的分子机制，生物学杂志，1994，5：7～12
79．向文胜，肖振平，赵长山 抗除草剂转基因作物．东北农业大学学报，1998，29(2)：201～208
80．肖凯，张荣铣外界条件对作物叶片衰老的影响．国外农学—麦类作物．1994，(3)：41～44
81．谢道昕，范云六，倪万潮，黄骏麒 苏云金芽孢杆菌杀虫晶体蛋白基因导入棉花获得转基因植物．中国科学(B辑)．1991，21(4)：367～373
82．徐明良，杨金水，葛扣麟 植物转基因的整合机制．植物生理学通讯，1996，32(3)：697～704
83．徐秋华 用RAPD标记分析我国陆地棉品种资源的遗传多样性：[硕士论文]．武汉：华中农业大学，2000
84．许耀，施骏，李宝健单、双子叶植物的代谢物调节农杆菌Vir区基因表达的研究，遗传学报，1993，20(1)：59～67
85．阎隆飞，刘国琴，肖兴国 从花粉肌动蛋白到作物雄性不育．科学通报，1999，44(23)：2471～2475
86．于晓红 朱勇清 种子特异表达ipt转基因棉花根和纤维的改变．植物学报．2000，42(2)：59～63
87．于元杰，尹承佾，沈法福，杨掀同，程立 应用植物分子育种技术选育棉花种质系．见：麦棉分子育种研究，成都：四川科学技术出版社，1995．第6～13页
88．喻树迅，范术丽，原日红，余学科，巩万奎 清除活性氧酶类对棉花早熟不早衰特性的遗
传影响，棉花学报，1999，11(2)：100—105
89．袁朝兴，丁静 水分胁迫对棉花叶片中IAA含量、IAA氧化酶和过氧化物酶活性的影响．植物生理学报．1990，2：179～184
90．岳建雄，石跃进，张慧军，王福社棉花试管苗“出管”移栽经验点滴．植物生理学通讯．1999，35(6)：482
91．张国栋，赵长山大豆抗除草剂特性的遗传及其利用．大豆科学，1991，10(4)：310～315
92．张家明，孙雪飘，郑学勤，张献龙，赵燕，刘金兰，孙济中 陆地棉愈伤诱导及胚胎发生能力的遗传分析，中国农业科学，1997，30(3)：36～43
93．张家明，孙济中，刘金兰，张献龙 陆地棉体细胞植株再生及其移栽技术研究．作物学报．1994，20(2)：210～215
94．张赛群 异戊烯基转移酶基因转化番茄及转基因植株再生：[硕士论文]．武汉：华中农业大学，1998
95．张献龙，孙济中，刘金兰 陆地棉体细胞胚胎发生与植株再生．遗传学报．1991，18(5)：461～467
96．张献龙，林双龙，吕复兵，董新国 陆地棉茎尖培养影响因素的研究．华中农业大学学报，1996，15(3)：210～214
97．张献龙，张家明，姚明镜，孙济中，刘金兰 棉花体细胞培养及其应用基础研究．华中农业大学学报，1993，12(5)：421—426
98．张宪政，陈凤玉，王荣富 主编．植物生理学实验技术．辽宁科学技术出版社．1994，66～69，184～186
99．郑泗军，吴吉祥，洪彩霞 棉花组织培养中畸形胚产生原因的分析。浙江农业大学学报，1993，19(2)：193—197
100．赵俊侠，焦改丽，李淑君，陈志贤 不同农杆菌菌株和棉花品种在转基因棉花中的应用研究．棉花学报．1997，9(4)：213～216
101．赵俊侠，齐宏力，焦改丽，陈志贤，南殿杰，杨苏龙，史俊东 棉花外源基因导入及转基因再生植株条件的研究．棉花学报．1998，10(4)：189～192
102．周光宇，龚蓁蓁，王自芬1979．远缘杂交的分子基础——DNA片段杂交假设的一个论证．遗传学报，6(4)：405～412
103．周光宇 分子育种—授粉后外源DNA导入植物技术，中国农业科学，1988，21(3)：1～6
104．周光宇 植物分子育种的兴起与展望，农业分子育种研究进展，中国农业科技出版社，1993：11～12
105．周岩，田颖川，吴标，莽克强 转雪花莲外源凝集素基因烟草对桃蚜的抑制作用．生物工
程学报，1998，14(1)：13～19
106．朱广廉，钟诲文，张爱琴编 小麦叶片在哀老过程中氧化脂质含量的变化．植物生理学实验．北京大学出版社．1990，245～248
107．朱卫民，吴敬音，佘建明，蔡小宁，朱祯，李向辉 棉花茎尖分生组织在微粒轰击法基因转化中的应用，江苏农业学报，1998，14(2)：74～79
108. A. Peri H. Kless,A. Blumenthal, G. Galili, and Galun. Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization of culture conditions and DNA-microprojectile delivery procedures, Mol Gen Genet, 1992, 235:279～284
109. Adang M J, Application of a Bacillus thuringiensis crystal protein for insect control. Molecular Strategies for Crop Protection, 1987, 345～353
110. Akama K, Puchta H, Hohn B Efficient Agrobacterium-mediated transformation of Arabidopsis thaliana using bar gene as selectable marker. Plant Cell Report, 1995,14; 450～454
111. Alejandro P V Expression of the hygromjcin B phosphotrasferase gene confers tolerance to the herbicide glyphosate. Plant Cell Report, 1995, 14: 482～487
112. Alfrid Nobel Drive, Hercukes, California Biolistic PDS-1000/He Particle Delivery System Instruction Manual. Bio Rad Laboratories, 94547 U.S.A. 1995
113. Allen R D, Trolinder NL Expression of superoxide dismutase in transgenic plants leads to increased stress tolerance. In: 1995 Beltwide Cotton conferences. [s.l.]:[s.n.], 1995.1136-1137
114. Altabellat T, Chrispeels M J Tobacco plants transformation with the bean α-ai gene express an inhibitor of insect α-amylase in their seeds. Plant Physiol, 1990,93:805～810
115. Barbieri L, Battelli M G, Stirpe F Ribosome-inactivating proteins from plants. Biochim. Biophys. Acta. 1993, 11 54:273～282
116. Batchvarova, V. Nikolaeva, S. Slavov, S. Bossolova, V. Valkov, S. Atanassova, S. Guelemerov, A. Atanassov, H. Anzai Transgenic tobacco cultivars resistant to Pseudomonas syringae pv. tabaci. Theor. Appl. Genet, 1998, 97:986～989
117. Bate NJ, Rothstein S J, Thorngson JE Expression of nuclear and chloroplast photosynthesis-specific genes during leave senescence. J Exp Bot,1991,42:801～811
118. Bayley, C, N. Trolinder, C. Ray, M. Morgan, J. E. Quisenberry, and D. W. Ow Engineering 2,4-D resistance into cotton. Theor. Appl. Genet. 1992,83:645～649
119. Beasly, C A In vitro culture of fertilized cotton ovules. Bioscience, 1971,21:906-907
120. Benedict J H, Ring D R,Sachs E S Influence of transgenic Bt cottons on tobacco budworm behavior, surival and plant injure.proceedings of cotton Beltiwide conferences,1992,599～601
121. Benfey P N, Chua N H Regulated gene in transgenic plants. Science, 1989, 244:174～181
122. Bouamama Efficiency of coupling biolistic and Agrobacterium in genetic transformation of tunisian autochthonous grapes, Agr. Med. Vol.2000, 130:223～227
123. Bradley D. The insecticidal Cry IB crystal protein of Bacillus thuringiensis subs. Has dual specificity to coleopteran and lepidopteran larvae. In: Abstracts of the ⅩⅩⅤ Annual meeting of the Society for Invertabrate pathology. Heidellbery, 1992, 234
124. Broer, I Stress inactivation of foreign genes in transgenic plants. Field Crop Research, 1996, 45(3),19～25
125. Buchanan-Wollaston V, Ainsworth C Leaf senescence in Brassica napus: cloning of senescence-related genes by subtractive hybridation. Plant Mol Biol, 1997,33:821～834
126. Buchanan-Wollaston V The molecular of leaf senescence. J Exp Bot, 1997,48:181～199
127. C. Zapata. S. H. Park. K. M. El-Zik. R. H. Smith Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex. Theor Appl Genet. 1999,98:252～256
128. Cao J, Duan X L, EcElroy, Cao, I., X. Duan, D. McElroy and R. Wu Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Reports, 1992, 11:586～591
129. Caryl A. Chlan, Junmin Lin, Jeffery W. Cary and Thomas E. Cleveland. A procedure for biolistic transformation and regeneration of transgenic cotton from meristematic tissue, Plant Molecular Report, 1995, 13(1): 31～37
130. Christou P, Ford TL, Kofron M Production of transgenic rice (Oryza sativa L.) plants from agronomically important Indica and Japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/technology, 1991,9:957～962
131. Clouse SD Molecular genetic studies confirm the role of brassinosteroids in plant growth and development. Plant J, 1996,10:1～8
132. Cook D, Rasche M, Eisnger W Regulation of ethylene biosynthesis and action in cut
carnation flower senescence by cytokinins. J Amer Soc Hort Sci, 1985, 110:24～27
133. Cousins Transformation of an Ausralian Cotton Cultivar: Prospects for Cotton Improvement through Genetic Engineering, Aust. J. Plant Physiol. 1991,18,481～494
134. Curtis I, He S, Scott C P, J Brian Power, Michael R Devey. Genomic male sterility in lettuce, a baseline for the production of F1 hybrids. Plant Sci, 1996, 113:113～119
135. D.C. Agrawal, A. K. Banerjee, R. R. Kolala, A. B.Dhage, A. V. Kulkarni, S. M. Nalawade, S. Hazra, K. V. Krishnamurthy, In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.), Plant Cell Rep, 1997, 16: 647652
136. Davidonis, G H and Hamilton, R H. Plant regeneration from callus tissues of Gossypium hirsutum L. Plant Scei. Lett, 1983,32:89～93
137. Davies KM, Grierson D Identification of cDNA clones for tomato (Lycopersicon esculenturn Mill.) mRNAs that accumulate during fruit ripening and leaf senescence in response to ethylene. Planta, 1989,179:73～80
138. Davies W J, Jianhua Z Root signals and the regulation of growth and development of plants in drying soil. Ann Rev Plant Physiol plant Mol Biol, 1991,42:55
139. Delong A, Calderon-Urrea A, Dellaporta SL Sex determination gene TASSELSEED2 of maize encodes a short-chain alcohol dehydrogenade required for stage-specific floral ofgan abortion. Cell,1993,74:757～768
140. Denis M, Delourme R, Gourret J P, Mariani C, Renard M Expression of engineered nuclear male sterility in Brassica napus. Plant Physiol, 1993, 107:1295～1304
141. Dennis Bidney, Chris Scelonge, Joanie Martich, Monique Burrus, Lynn Sims and Gary Huffiman, Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens, Plant Molecular Biology, 1992,18:301～313
142. Down R E, Gatehouse A M R, Hamilton W D O, et al Snowdrop lectin inhibits development and decreases fecundity of glasshouse potato aphid (Aulacorthum solani) when administered in vitro and via transgenic plants both in laboratory and glasshouse trails. J. Insect Physiol, 1996, 42:1035～1045
143. Downes B P, Crowell D N Cytokinin regulates the expression of a soybean β-expansin gene by a post-transcriptional mechanism. Plant Mol Biol, 1998,37:437～444
144. Ebrahim Firoozabably David L, DeBoer, Donald J. Merlo, Edward L. Halk, Lorraine N. Amerson, Kay E. Rashka and Elizabeth E. Murray. Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants, Plant Molecular Biology, 1987, 10: 105～116
145. Eliseu S, Figucrira F, Lucio F A Transformation of pototo (Solanum fuberosum) cv. Mantigueira using Agrobacterium tumefaciens and evaluation of herbicide resistance. Plant Cell Report, 1994, 13:666～670
146. Firoozabady, E., D.L. DeBoer, D.J. Merlo, E.L. Halk, L.N. Amerson, K.E. Rashka, and E.E. Murray Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants. Plant. Mol. Biol. 1987,10:105～116
147. Frederick J. Perlak, Randy W. Deaton, Toni A. Armstrong, Roy L. Fuchs, Steven R. Sims, John T. Greenplate and David A. Fischhoff. Insect resistant cotton plants, Bio/Technology, 1990,8:939-942.
148. Fullner K J, Lara J C, Nester E W Pilus assembly by Agrobacterium T-DNA transfer genes. Science, 1996,273:1107～1109
149. Gan S, Amasino RM Inhibition of leaf senescence by autoregulated production of cytokinin.Sci. 1995, 270:1986～1988
150. Gan S, Amasino RM Making senescence: molecular gentic regulation and manipulation of leaf senescince. Plant Physiol, 1997,113:313～319
151. Ganesan Sunilkumar and Keerti S. Rathore. Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration, Molecular Breeding, 2001,8:37～52
152. Gasser C S, Fraiey R T Genetically engineering plants for crop improvement. Science, 1989, 244:1293～1299
153. Genevieve Hansen Constitutive expression of the virulence gene improve the efficiency of plant transformation by Agrobacterium, Plant Biology, 1994, 91: 7603～7607
154. Genevieve Hansen and Mary-Dell Chilton. "Agrolistic" transformation of plant cells integration of T-strands generated in planta. Proc Natl Acad Sci USA, 1996,93:14978-14983
155. Georage C. Allen, Gerald Hall, Jr., Susan Michalowski, Winnell Newman, Steven Spiker, Arthur K. Weissinger, and William F. Thompson, High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco, Plant Cell, 1996, 8(5), 899～913
156. Ghosh B, Biswas G C, Lglesias V A, Datta S K, Potrykus L Transgenic indica rice plants obtained by direct gene transfer to protoplasts, J.BIOTECH, 1994, 32:1～10
157. Gosset DR et al Antioxidant status in salt stressed cotton. Beltwide Cotton Conferences of America 1992, 1036-1
158. Grabam IA, Leaver CJ, Smith SM. Induction of malate wynthas gene expression in senescent and detached organs of cucumber. Plant Cell, 1992, 4:349～357
159. Grbic V, Bleecker AB Ethylene regulates the timing of leaf senescence in Arabidopsis. Plant J, 1995, 8:595～692
160. Greg Keller, Lori Spatola, Demis Mccabe, Brian Martinell, William, Swain, Maliyakal E. John. Transgenic cotton resistant to herbicide bialaphos,Transgenic Research, 1997,6:385～392
161. Gupta S K, Alok K. Sdvastava, Pradhyumna K. Singh and Rakesh Tuli. In vitro proliferation of shoots and regeneration of cotton, Plant Cell Tiss Organ Cul, 1997, 51: 149～152
162. HaKe S Unraveling the knots in plant development. Trends in Genetics.1992,8:109～114
163. Hain R, Reif H J, Krause E, Langebartels R, Kindl H, Vornam B, Wiese W, Schmelzer E,Schreier P H, Stocker R H,Stenzel K. Disease resistance results from foreige phytoalexin expression in a novel plant. Nature, 1993, 361:153～156
164. Hamilton A J, Lycett GW, Grierson D.Antisense gene that inhibbits synthesis of the hormonce ethylene in transgenic plants. Nature, 1990,346:284～287
165. Hanfrey C, Fife M, Buchanan-Wollaston V Leaf senescence in Brassica napas: expression of genes encoding pathogenesis-related proteins. Plant Mol Biol. 1996,30: 597～609
166. Hensel LL, Grbic V, Baumgarten DA and Anthony B. Bleecker. Developmental and age-related process that influence the longevity and senescence of photosynthetic tissues in Arabidopsis Plant Cell, 1993,5:553～564
167. Hooykaas P J J, Schilperoort R A Agrobacterium and plant genetic engineering. Plant Mol Biol, 1992,19:15～38
168. Hooykaas P J J, Beijersbergen A G M The virulence system of Agrobacterium tumefaciens. Annu Rev Phytopathol, 1994, 32:157～179
169. Horsh R B, Fry J E, Hoffmann N L, Eichholtz. D, Rogers. S G, Fraleg R T. A simple and general method for transferring genes into plants. Scince, 1985, 227:1229～1231
170. Hradecka D, Staszkova L Influence of the application of cytokinin and nitrogen fertilizer on spring wheat. Rostlinna Vyroba, 1996,42(7): 301～306
171. Huang Y, Nordeen R O, Di M, L. D. Owens, J.H. Mcbeath. Expression of an engineered
Cecropin gene cassette in transgenic tobacco plants confers disease resistance to Pseudomonas syringae pv. tabaci. Phytopathology, 1997, 87:494～499
172. Isaac Jone, Rfarrell Drake, Aldo Farrel, Wendy Cooper, Pam Lee, Peter Horton and Don Grierson. Delayed leaf senescence in ethylene-deficient ACC oxidase antisense tomato plants: molecuar and physiolo-gical analysis. Plant J, 1995,7:483～490
173. Ivanova A P Stefanov K L, Yordanov I T Effect of cytokinin and cytokinin 4PU-30 on the lipik composition of water stressed bean plants. Biologia Plantarum.1998, 41(1): 155～156
174. J.K. Hemphill C. G. A. Maier K. D. Chapman. Rapid in vitro plant regeneration of cotton (Gossypium hirsutum L.), Plant Cell Rep, 1998, 17:273～278
175. J. Ke R. Kham T. Johnson D. A. Somers A. Das. High-efficiency gene transfer to recalcitrant plants by Agrobacterium tumfaciens, Plant Cell Reports,2001,20:150～156
176. Jach G, Gornhardt B, Mundy J, Guido Jach, Birgit Grnhard, John Mundy, Jürgen Logemann, Elke Pinsdorf, Robert Leah, Jeff Schell and Christoph Maas Enhanced quantitative resistance against fungal disease by combinatoril expression of defferent barley antifungal proteins in transgenic tobacco. Plant J, 1995, 8:97～109
177. Jefferey W. Pollard and John M. Walker Plant Cell and Tissue Culture: Meth-ods in Molecular Biology.6. Hummana Press. Clifton, New Jersey
178. Jean Gould, Sharon Banister, Osamu Hasegawa, Michal Fahima, Roberta H.Smith. Regeneration of Gossypium hirsutum and G. barbadense from shoot apex tissues for transformation, Plant Cell Rep, 1991, 10:12～16
179. Jiang CZ, Rodermel SR, Shibles RM Photosynthesis, rubisco activity and amount, and their regulation by transcription in sdndscing soybean leaves. Plant Physiol, 1993,101:105～112
180. John J. Finer, Transformation of cotton (Gossypium hirsutum L.) via particle bombardment, Plant Cell Reports, 1990, 8:586～589
181. Jongedijk E, Tigelaar H, van Roekel J S C, Sandra A Bres_Vloemans, llma Dekker, Peter J. M, Van den Elzen, Ben J. C. Comelissen, Leo S. Melchers. Synergistic activity of chitinases and β-1,3-glucanases enhances fungal resistance in transgenic tomato plants. Euphytica, 1995, 85:173～180
182. Kawami N and W Keller. Transgenic cotton resistant to herbicide bialaphos, Transgenic Research, 1997,6:385～392atanabe A Change in gene expression in radish cotyledons during dark-induced senescence. Plant Cell Physiol.1988a,29:33～42
183. Kim DJ, Smith SM Molecular cloning of cucumber PEP carboxykinase and developmental regulation of gene expression. Plant Mol Biol.1994a,26:423～434
184. Kim DJ, Smith SM Expression of a single gene encoding microbody NAD-malate Dehydrogenade during glyoxysome and peroxisome development in cucumber. Plant Mol Biol. 1994b, 26:1833～1841
185. K. Rajasekaran High-frequency stable transformation of cotton (Gossypium hirsutum L.) by particle bombardment of embryogenic cell suspension cultures, Plant Cell Reports, 2000, 19: 539～545
186. Klein T. M, E. D. Wolft, R. Wu and C. Sanford. High-velocity microprojectile for delivery of mucleic acids into living cells, Nature, 1987, 327:70～73
187. Koizumi M, Yaneguchi-shinozaki K, Tsuji H et al Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana. Gene, 1993,129:175～182
188. Kramer C, DiMaio J, Carswell G K Selection of transformed protoplast-derived Zea mays colonies with phosphinothdcin and a novel assay using the PH indicator cholorophenol red. Planta, 1993, 190:454～458
189. Kranz E, Lorz H Micromanipulation and in vitro fertilization with single pollen grains of maize Sex Plant Reprod, 1990,3:160～169
190. Lane B G, Dunwell J M, Ray J A, Mark R. Schmitt, Andrew C. Cuming. Germin. A protein marker of early plant development, is an oxalate oxidase, J. Biol. Chem. 1993, 268(17): 12239～12242
191. Lee J H, Montagu M V, Verbruggen N A highly conserved kinase is an essential component for stress tolerance in yeast and plant cells. Proc. Natl. Acad. Sci. USA, 1999, 96: 5873～5877
192. Lee L Y, Gelvin S B, Kado CI pSa causes oncogenic suppression of Agrobacterium by inhibiting VirE2 protein export. J Bacteriol, 1999, 181(1): 186～196
193. Lejeune P, Prinsen E. Onckelen H V, Bernier C, Van O H Hormonal control of ear abortion in a stress-sensitive maize inbred. Australian J Plant Physiol, 1998,25(4): 481～488
194. Li fuguang, Study on Insect-resistant Transgenic Cotton Harbouring Double-gene and Its resistance to Insect Pests, Chinese Agricultural Sciences, 2001, No.1, 70～76
195. Lin, W., Anuratha, C. S., Datta, K., Potrykus, I., Muthukrishnan, S. and Datta, S. K Genetic engineering of rice for resistance to sheath bilght. Bio/Technology,
1995,13:686～691
196. Linda L. Hensel, Vojislava Grbic, David A. Baumgarten, Anthony B. Bleeker, Developmental and age-related process that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell, 1993,5:553～564
197. Lohman KN, Gan S, John MC and Amasino. R. M. Molecular analysis of natural leaf senescene in Arabidopsis thaliana. Physiol Planta, 1994, 92: 322～328
198. Mariani C, Gossele V, Beuckeleer M D, et al A chimaeric ribonuclease inhibitor gene restores fertility to male sterile plants. Nature, 1992, 357:384～387
199. Matthews BF, Abdul-Baki AA, Saunders JA Expression of a foreign gene in electropotated pollen grains of tobacco. Sex Plant Reprod, 1990,3:147～151
200. Mayerhofer R, Konz-Kalman Z, Nawrath C, Bakkeren G, Crameri A, Angelis K, Redei GP, Schell J, Hohn B, and Koncz C T-DNA intrgration: amode of illegitimate recombination in plants. The EMBO J, 1991, 10(3): 697～704
201. Mazur B J, Carl F S The development of herbicide-resistant crops. Annu Plant Physiol & Plant Mol. Biol, 1989, 40:441～470
202. McCabe DE, Christou P Direct DNA transfer using electric discharge particle acceleration technology. Plant cell and Organ Culture, 1993,33:227～236
203. McCabe D. E. and Martinell B.J. Transformation of elite cotton cultivar via particle bombardment of meristems, Bio-technology, 1993, 11(5): 596～598
204. Michael J. Adang, Michael J. Staver, Thomas A. Rocheleau, Jacqui Leighton, Richard F. Barker and David V. Thompso, Staver M J et al Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subs. Kurstaki HD-73 and their toxicity to Manduca Sexta. Gene, 1985, 36 289～300
205. Morre J L, Hugo R. Permingeat, Maria V. Romagnoli, Cinitia M. Heisterborg and Ruben H. Vallejos Multiple shoot induction and plant regeneration from embryonic axes of cotton, Plant Cell Tiss Organ Cult, 1998, 54:131～136
206. Murray F R, Lewellyn D J, Peacock W J Isolation of the glucose oxidase gene form Talaromyces flavus and charactrisation of ite role in the biocontrol of Verticillium dahliae. Current Genetics, 1997, 32:367～375
207. Nakamura Y, Sawada H, obayashi S, Nakajima I, Yoshikawa M Expression of soybean β-1,3-endogiucanase cDNA and effect on disease tolerance in kiwifruit plants. Plant Cell Report, 1999,18:527～532
208. P. Venkatachalam N, Geetha, Abha Khandelwal, M. S. Shaila and G. Lakshmi Sita. Agtobacteium-mediated genetic transformation and regeneration of transgenic plants from cotyledon explants of groundnut (Arachis Hypogaeal.) via somatic embrygenesis, Current Science, 2000, 78 (9): 1130～1136
209. Paterson A H, Brubaker C L, Wendel J G Arapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis. Plant Mol Biol Rep, 1993,11:122～127
210. Paul Umbeck, Gail Johnson, Ken Barton, and Will Swain. Genetically transformed cotton (Gossypium hirsutum L.) plants, Bio-technology, 1987, 5:263～266
211. Paula Elomaa Yrjo Helariutta. Robert J. Griesbach Mika Kotilainen. Pauli Seppanen. Teemu H. Teeri. transgene inactivation in Petunia hybrida is influenced by the properties of the foreign gene. Molecular and General Genetics, 1995, 248, 649～656
212. Philip N. Benfey and Nam-Hai Chua, Regulated Genes in Transgenic Plant. Science, 1989, 224, 174～181
213. Powell-Abel, P.P, Nelson, R.S, De, B, Hoffmann, N, Rogers, S.G, Fraley, R.T, and Beachy, R.N Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science1986, 232:738-743
214. Price, H J and smith, R. H Somatic embryogenesis in suspension culture of Gossypium Klotzschiarum Anderss. Plant, 1979,305～307
215. Purnhauser L. Medgyesy P, Czako M Philio J. Dix and Laszlo Marton. Stimulation of shoot regeneration in Triticum aestivum and Nicotiana Plumbaguifolia Viiv. tissue culture uding the ethylene inhibitor AgNO3. Plant Cell Rep. 1987, 6:1～4
216. Simon C. Deroles and Richard C. Gardner Analysis of the T-DNA structure in a large number of transgenic petunias generated by agrobaccterium-mediated transformation. Plant Mol. Biol. 1988, 11,365～377
217. R.B. Horsch J. E. Fry N.L.Hoffmann D. Eichholtz S.G.Rogers R.T. Fraley. A simple and general method for transferring genes into plants, Science, 1985,217:1229～1231
218. Reynolds D B, Crawford S H, Rogers R L Weed control in transgenic Buctril herbicide-tolerant cotton. Louisiana Agri, 1994, 37(3): 13～14
219. Rupp H. M Cotykinin overproduction in transgenic Arabidopsis thaliana. The 8th Int'l. Cong. on Arabidopsis thaliana Rech. Wisconsin, July 1997
220. Ryu SB, Wang XM Expression of phospholipase-D during castor bean leaf senescene. Plant Physiol.1995,108:713～719
221. S.H. Park, Short T-DNA or additional virulence genes improve Agrobacterium--mediated transformation, Theor Appl Genet, 2000, 101:1015～1020
222. Saeed N A, Yusuf Zafar and Kauser A. Malik. A simple procedure of Gossipium meristem shoot tip culture, Plant Cell Tiss Organ Cul, 1997, 51:201～207
223. Sanford J.C Deliver of substance into cells and tissues using aparticle bombardment, Process J Part Sci Technol, 1987, 5:27～37
224. Schuler T H, Poppy G M Insect-resistant transgenic plants. Tibtech, 1998,16:168～175
225. Smart C. M, Hosken S. E, Thomas H. John A. Greavea, Bruce G, Blair and Wolfgang Schuch.The timing of maize leaf senescence and characterization of senescence-related cDNAs. Physiol Plant, 1995,93:673～682
226. Smigocki. A., Neal. J. W. Jr., McCanna, I. and Douglass, L Cytokinin-mediated insect resistance in Nicotiana plants transformed with the ipt gene. Plant Molecular Biology. 1993, 23:325-335.
227. Smith CR, Saunders JA Expression of GUS and CAT activities using electransformed pollen. Plant Sci 1994,104:49～58
228. Song W Y, Wang G L, Ronald P, Wen-Yuan Song, Guo-Liang Wang, Li-Li Chen, Han-Suk Kim, Li-Ya Pi, Tom Holsten, J. Gardner, Bei Wang, Wen-Xue Zhai, Li-Huang Zhu, Claude Fauquet, and Pamela Ronald Areceptor kinase-like protein encoded by rice disease resistance gene, Xa21. Science, 1995, 270:1804～1806
229. Songstad. D, Duncan. DR, Wildholm. J, M Effect of 1-aminocyclopropane 2-carboxylic cid. silver nitrate.and norbornadiene on plant regeneration from maize callus cultures. Plant Cell Rep. 1988,7:262～265
230. Stoger E, William S, Christou P Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid sitobion avenae. Molecular Breeding, 1999, 5(1):65～73
231. Strauss S H, Rottmann W H, Brunner A M, Lorraine A Genetic engineering of reproductive sterility in forest trees. Molecular Breeding, 1995, 1:5～26
232. Sundberg, C., L. Meek, K. Carroll, A. Das and W. Ream VirE1 protein mediates export of the single-stranded DNA-binding protein VirE2 from Agrobacterium tumefaciens into plant cells. J Bacteriol, 1996, 178(4): 1207～1212
233. Tabei Y, Kitade S, Nishizawa Y, Kikuchi N, Eayano T, Hibi T, Akutsu K Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea). Plant Cell Reports, 1998, 17:159～164
234. Takaichi M, Oeda K Transgenic carrots with enhanced resistance against two major pathogens, Etysiphe heraclei and Alternaria dauci. Plant Science, 2000, 153:135～144
235. Taylor CB, Bariola PA, Delcardare SB Ronald T. Raines, Pamela J. Green. RNS2: a senescence-associated Rnase of Arobidopsis that diverged from the S-Rnases before speciation. Proc Natl Acad Sci USA. 1993,90:5118～5122
236. Thea A. Wilkins Kanniah Rajasekaran and David M. Anderson. Cotton biotechnology, Critical Reviens in Plant Sciences, 2000, 19(6): 511～550
237. Thomas H. Sid a Mendelian locus controlling thylakoid membrane disassemhlyin senescencing leaves of Festuca pratensis. Theoretical and Applied Genetics. 1987,73:551～555
238. Thompson C, Dunwell J M, Johnstone C E, Lay V, Ray J, Schmitt M, Watson H, Nisbet G Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase. Euphytica, 1995, 85:169～172
239. Thomzik, JE, Stenzel, K., Stocker, R., Schreier, PH, Hain, R., and Stahl, DJ Synthesis of a grapevine phytoalexin in transgenic tomatoes (Lycopersicon esculentum) conditions resistance against phytophthora infestns. Physiol Nol. Plant Pathol, 1997,51:265～278
240. Touraew A, Stoger E, Voronin V, Hebede-Bors E Plant male germ line transformation. Plant J, 1997,12:949～956
241. Trick H N, Finer J J. SAAT: soniation-assisted Agrobaterium-mediated transformation. Transgenic Researh, 1997, 6:329～336
242. Trolinder, N L Somatic embryogenesis in Cell suspension cultures of G.hirsutum. procedlings Beltwide cotton production research conference. 1984,46
243. Trolinder N Land J. R. Goodin. Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.), Plant Cell Rep, 1988, 6:231～234
244. Twell D, Klein T, Fromm ME, McCormick S Transient expression of chimeric genes dilivered into pollen by microprojectile bombardment. Plant Physiol, 1989:1270～1274
245. Umbeck, P., G. Johnson, K. Barton, and W. Swain Genetically transformed cotton (Gossypium hirsutum L.) plants. Bio/Technology. 1987,5:263～266
246. Vermenlen A, Vaucheret H Agrobacterium mediated transfer of a mutant Arabidopsis acetolacte synthase gene confers resistance to chlorsulfuron in chicory. Plant Cell Report, 1992, 11:243～247
247. W. Cherdshewasart, G.B. Ghareti-chhetri, M. W. Saul, M. Jacobs and I. Negrutiu. Expression instability and genetic disorders in transgenic Nicotiana plumbaginifolia plants. Transgenic Research, 1993 2(6), 307～320
248. Watanabe H, Abe K, Emori Yhosoyama, and Soichi Ami. Molecular cloning and giberellion-induced expression of multople cysteine proteinases of rice seeds (oryzains). J Biol Chem. 1991,266:16897～16902
249. Willy Dillen, Janniek De Clercq, Jyoti Kapila, Mukund Zambre, Marc Van Montagu and Geert Angenon. The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer to plants, The Plant Journal, 1997,12(6):1459～1463
250. Wu G, Shortt B J, Lawrence E B, Levine B, Fitzsimmons K. C, and Shah D. M Disease resistance conferred by expression of a gene encoding H2O2-generating glucose potato plants. Plant Cell, 1995, 7:1357～1368
251. Xia G M, Li Z Y, He C X, Chen HM, Ricard Brettell. Transgenic plant regeneration from wheat (Triticum aestivum L.) mediated by Agrobacterium tumefaciens. 植物生理学报，1999, 25(1):22～28
252. Yi-chang wang Theodore M. Klein, Michael Fromm, Jun Cao, John C. Sanford and Ray Wu. Transient expression of foreign gene in rice, wheat and soybean cell following particle bombardment, Plant Molecular Biology, 1988, 11:433～439
253. Zambryske P Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regulation apacity. EMBO J., 1983, 2:2143～2150
254. Zambryski P C Chronicles from the Agrobacterium-plant cell DNA transfer story. Annu Rev Plant Physiol Plant Mol Biol, 1992, 43:465～490
255. Zhou H, Arrowsmith J.W, Fromm M.E, Hironaka C.M, Taylor M.L, Rodriguez D, Pajeay M.E, Brown S.M, Santino C.G, and Fry J.E Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Report, 1995, 15:159～163
256. Zou J T, Zhan X Y, Wu H M, et al Characterization of a rice pollen-specific gene and its expression. Am J Bot, 1994,81:552～561
257. Zupan J R, Zambryski P C Transfer of T-DNA from Agrobacterium to the plant cell. Plant Physiol, 1995,171:1041～1047