玫参胶囊治疗围绝经期综合征的实验研究
摘要
目的:观察玫参胶囊对去卵巢大鼠血清雌激素、下丘脑单胺类神经递质水平及血清脂质及抗氧化指标的影响。
方法:以去卵巢大鼠为动物模型,更年欣胶囊为阳性对照药物,观察玫参胶囊治疗4周后大鼠血清性激素、下丘脑单胺类神经递质水平变化及玫参胶囊用药12周对大鼠体重变化、血脂、载脂蛋白及脂质过氧化物含量的影响。
结果:与模型对照组相比,玫参胶囊能明显升高去卵巢大鼠血清雌二醇(E2)水平,降低血清卵泡刺激素(FSH)、黄体生成激素(LH)水平;有效降低去卵巢大鼠5-羟色胺(5-HT)和5-羟吲哚乙酸(5-HIAA)水平,并升高NE和DA的含量。玫参胶囊各剂量组大鼠体重均明显降低,以高、中剂量组为著;玫参胶囊高、中剂量组大鼠总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-c)水平均明显降低,低剂量组TC亦降低,高剂量组高密度脂蛋白胆固醇(HDL-c)升高;玫参胶囊高、中剂量组大鼠超氧化物歧化酶(SOD)活性明显升高,丙二醛(MDA)含量明显下降。差异均有显著性意义(p<0.01或p<0.05)。
结论:玫参胶囊通过对去卵巢大鼠内分泌系统与神经系统的调节,以达到稳定机体内环境,缓解围绝经期症状的作用;玫参胶囊亦能有效调节去卵巢大鼠紊乱的血脂,提示其具有防治绝经期综合征患者动脉粥样硬化或冠心病的作用。
Objective To explore the effects of Meishen capsule on serous sexual hormone, monoamine neurotransmitter content of hypothalamus and blood-lipid,anti-oxygen index in ovariectomized rats.
Methods Using ovariectomized rats as model and Gengnianxin capsule as positive control drug,The change of serous sexual hormone,monoamine neurotransmitter content of hypothalamus at 4w after intervening with Meishen capsule,the change of bodyweight and the contents of blood-lipid(BL),anti-oxygen index after 12w were obversed in ovariectomized rats.
Results Compared with model group,Meishen capsule could obviously increase the content of Estradiol(E2) and decrease that of Follicl-stimula ting Hormone(FSH), Luteinizing Hormone(LH).In addition,the prescription could also effectively decrease the index of 5-hydroxytrypta mine(5-HT),5-hydroxyindole acetic acid(5-HIAA) and increase that of norepinephrine(NE) and dopamine(DA).Every dosage group of Meishen capsule could decrease the weight of rats,especially the high and middle group.The values of total cholesterol(TC),low density lipoprotein cholesterol(LDL-C) in both high and middle dosage group could decrease,the content of high density lipoprotein cholesterol(HDL-C) in the former group also could increase,the values of TC in the low dosage group could decrease.The activity of superoxide dismutase(SOD) both in high and middle dosage group could increase,in which the contents of Maleic Dialdehyde (MDA)could decrease.Compared with the three groups,there was a significant difference.
Conclusion Meishen capsule could stabilize organism internal environment and relieve the perimenopausal symptoms by accommodating cryptorrheic and nervous system of ovariectomized rats.It also can accommodate confused blood-lipid of ovariectomized rats,which indicate that it could prevent the patients from getting atherosclerosis or coronary artery disease(CHD) and treat the syndrome.
方法:以去卵巢大鼠为动物模型,更年欣胶囊为阳性对照药物,观察玫参胶囊治疗4周后大鼠血清性激素、下丘脑单胺类神经递质水平变化及玫参胶囊用药12周对大鼠体重变化、血脂、载脂蛋白及脂质过氧化物含量的影响。
结果:与模型对照组相比,玫参胶囊能明显升高去卵巢大鼠血清雌二醇(E2)水平,降低血清卵泡刺激素(FSH)、黄体生成激素(LH)水平;有效降低去卵巢大鼠5-羟色胺(5-HT)和5-羟吲哚乙酸(5-HIAA)水平,并升高NE和DA的含量。玫参胶囊各剂量组大鼠体重均明显降低,以高、中剂量组为著;玫参胶囊高、中剂量组大鼠总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-c)水平均明显降低,低剂量组TC亦降低,高剂量组高密度脂蛋白胆固醇(HDL-c)升高;玫参胶囊高、中剂量组大鼠超氧化物歧化酶(SOD)活性明显升高,丙二醛(MDA)含量明显下降。差异均有显著性意义(p<0.01或p<0.05)。
结论:玫参胶囊通过对去卵巢大鼠内分泌系统与神经系统的调节,以达到稳定机体内环境,缓解围绝经期症状的作用;玫参胶囊亦能有效调节去卵巢大鼠紊乱的血脂,提示其具有防治绝经期综合征患者动脉粥样硬化或冠心病的作用。
Objective To explore the effects of Meishen capsule on serous sexual hormone, monoamine neurotransmitter content of hypothalamus and blood-lipid,anti-oxygen index in ovariectomized rats.
Methods Using ovariectomized rats as model and Gengnianxin capsule as positive control drug,The change of serous sexual hormone,monoamine neurotransmitter content of hypothalamus at 4w after intervening with Meishen capsule,the change of bodyweight and the contents of blood-lipid(BL),anti-oxygen index after 12w were obversed in ovariectomized rats.
Results Compared with model group,Meishen capsule could obviously increase the content of Estradiol(E2) and decrease that of Follicl-stimula ting Hormone(FSH), Luteinizing Hormone(LH).In addition,the prescription could also effectively decrease the index of 5-hydroxytrypta mine(5-HT),5-hydroxyindole acetic acid(5-HIAA) and increase that of norepinephrine(NE) and dopamine(DA).Every dosage group of Meishen capsule could decrease the weight of rats,especially the high and middle group.The values of total cholesterol(TC),low density lipoprotein cholesterol(LDL-C) in both high and middle dosage group could decrease,the content of high density lipoprotein cholesterol(HDL-C) in the former group also could increase,the values of TC in the low dosage group could decrease.The activity of superoxide dismutase(SOD) both in high and middle dosage group could increase,in which the contents of Maleic Dialdehyde (MDA)could decrease.Compared with the three groups,there was a significant difference.
Conclusion Meishen capsule could stabilize organism internal environment and relieve the perimenopausal symptoms by accommodating cryptorrheic and nervous system of ovariectomized rats.It also can accommodate confused blood-lipid of ovariectomized rats,which indicate that it could prevent the patients from getting atherosclerosis or coronary artery disease(CHD) and treat the syndrome.
引文
[1]卫生部继续医学教育委员会组编女性生殖内分泌性激素补充疗法[M].北京医科大学中国协和医科大学联合出版社,1999年(第一版),34.
[2]张涛,叶少剑等.药食两用中药复方对去卵巢大鼠血脂的调节作用[J].时珍国医国药,2005,16(9):826-827.
[3]宋.陈自明原著明.薛己校注《校注妇人良方》注释[M].第一版南昌:江西人民出版社,1983,3.
[4]明.张景岳著景岳全书[M].第一版,北京:人民卫生出版社,1991,828.
[5]王小云,沈碧琼.更年期综合征临床辨治思路[J].黑龙江中医药,2001,(2):8-9.
[6]薛静燕.健脾升清饮治疗绝经前后诸症62例[J].南京中医药大学学报,2002,18(3):186-197.
[7]余庆.脾胃阴火与更年期综合征[J].福建中医学院学报,1999,9(3):34-36.
[8]谈勇,许小凤,卢苏.绝经前后诸症心肾病机探析[J].南京中医药大学学报(自然科学版),2001,17(6):340-342.
[9]陆启滨.更年期综合征病因病机探源[J].中医药学刊,2001,19(2):139-140.
[10]李庆生.治疗妇女更年期综合征应重视柔肝疏肝.气阴双调[J].云南中医中药杂志,1997,18(3):4.
[11]叶燕萍.女性更年期综合征病机及辩证分型分型的研究[J].江苏中医,2000,211(8):18-19.
[12]夏桂成主编.围绝经期综合征.中医妇科理论与实践[M].北京:人民卫生出版社,2003,280-287.
[13]刘晓伟,邓虹蛛.更年期综合征中医辨治研究进展[J].现代中西医结合杂志,2001,10(8):792.
[14]湖波.肾虚血瘀是围绝经期综合征的重要机制[J].中医药学刊,2005,9(23):1671.
[15]王光辉,薛居宏.更年期综合征治疗八法[J].四川中医,2000,18(7):10-11.
[16]陈晓明.分型辨治更年期综合征126例[J].甘肃中医学院学报,1998,15(3):30.
[17]夏融.更年期综合征侧重治心的理论与实践[J].江苏中医,1996,17(2):41-43.
[18]陈彩云,范洪涛.导师熊铺倡治疗妇女更年期综合征经验总结[J].云南中医药杂志,2002,23(1):5
[19]王春生.更年期综合症侧重从肺论治[J].辽宁中医杂志,1989,(3):23-24.
[20]张颖,褚贵保.倪宗咖治疗更年期综合征经验[J].实用中医药杂志,2001,17(10):34.
[21]叶丽霞,严以炳,潘红玫等.中医治疗更年期综合征86例疗效观察[J].长春中医学院学报,2001,17(3):35.
[22]张华,张淑芳,李国鹏.中西医结合治疗妇女更年期综合征206例[J].陕西中医,2001,22(11):659.
[23]张立.妇女更年期综合征的辨证分型与治疗[J].中医药学刊,2002,20(4):534.
[24]周焕凤,白文成.从肝肾论治妇女更年期综合征36例[J].陕西中医,2001,22(11):658.
[25]李得萍.更年期综合征辨治体会[J].实用中医药杂志,2002,9(18-9):34-35.
[26]李莉,孙竹春.左归丸治疗妇女更年期综合征72例[J].上海中医药杂志,2001,35(3):26-27.
[27]舒晓春等.龙风宝胶囊治疗更年期综合征50例[J].陕西中医,2003,23(8):715.
[28]李琼,舒仪琼,赖慧红等.镇肝熄风汤治疗围绝经期综合征的临床疗效观察[J].中国中西医结合杂志(英文版),2003,9(1):63-66.
[29]田晓娥.二仙汤加味治疗围绝经期综合征[J].Journal of Medical Forum,2004,25(2):57.
[30]王书杰,王丽萍,李丽荣.佳蓉片治疗更年期综合征疗效观察[J].时珍国医国药,2003,14(9):549.
[31]龚瑾,黄家茹.血府逐瘀胶囊治疗更年期综合征106例临床观察[J].Beijing JournalofTCM,2004,23(2):128.
[32]蔡雪芬等.更年宁心胶囊治疗更年期综合征65例[J].中国中西医结合杂志,2003,23(11):875.
[33]李国安,俞吟.针刺治疗女性更年期综合征的临床研究[J].上海针灸杂志,2002,21(3):6-8.
[34]孙敬青.走罐疗法治疗更年期综合征50例临床观察[J].针灸临床杂志,1999,15(7):31-32.
[35]孙冬梅,吴富东,单秋华,王金玲.耳穴贴压治疗女性更年期综合征的临床观察[J].针灸临床杂志,2003,19(12):32-35.
[36]潘红玲,许天兵.穴位注射治疗更年期综合征临床观察[J].针灸临床观察,1998,14(11):14.
[37]黎烈荣.更年期综合征患者情绪异常如何治疗[J].中医杂志,1998,39(1):54.
[1]徐苓等.围绝经期及绝经过渡期的定义及激素补充治疗[J].实用妇产科杂志,1999,15(4):177-178.
[2]卫生部继续教育委员会编.女性生殖内分泌性激素补充疗法[M].北京:中国协和医科大学出版社,1999.
[3]顾美皎.现代妇产科学[M].人民军医出版社,2002,1222.
[4]刘敏如,吴克明.世界传统医妇科学[M].科学技术出版社,1999,235.
[5]郎景和,向阳主编.高级医师案头丛书妇产科学[M].北京:中国协和医科大学出版社,2001:340.
[6]邓小虹,张松文.北京地区更年期妇女健康状况的流行病学调查[J].北京医学.2002,24(4):235-238:13.
[7]Fujimoto VY,Bremenr WJ,Battaglia DE,et al.The anterior pituitary response toagonadotropin-releasing hormone challenge test in normal older reproductive-age women[J].Fertil Steri,1996,6:539.
[8]步世忠,等.更年健上调老年雌性大鼠下丘脑雌激素受体mRNA表达对P物质和β-内啡肽的影响[J].中国中西医结合杂志,1998,18(1):28-30.
[9]范红霞,万力生,黎烈荣.更年安怡片对去卵巢大鼠脑组织中单胺类神经递质含量的影响[J].中医研究,1999,12(6):11-13.
[10]胡随瑜,尤劲松,向群辉等.地贞颗粒对“更年期”拟阴虚内热证模型大鼠的影响[J].中药新药与临床药理,2000,11(3):143-145.
[11]Brown ER Harlan RE,Krause LE.Gomadal steroi dregula 2tiono facidsin theratan terior pituitary and hypothalamus[J].Endocrinology,1990,126:330-340.
[12]吕肖锋,叶雪清.绝经后妇女血β-内啡肽及其抗体改变[J].中国实验临床免疫学杂志,1993,5(4):37.
[13]王跃清等.β-内啡肽对更年期妇女潮热的影响[J].实用妇产科杂志,1999,15(6):321.
[14]江仙远,陈友香,侯安继.更年康片治疗围绝经期综合征的机理研究[J].中药药理与临床,2001,17(1):36-38.
[15]王大增,朱燕青,李燕萍等.更年舒治疗妇女更年期综合征的临床及实验研究[J].中国中西医结合杂志,1994,14(7):396-399.
[16]HirikawaK,UtsuyamaM,KasiM,etal.Aging and immunocity[J].Acta Pathopn,2002,42(8):537-540.
[17]张雅萍,王秀霞.坤宁安丸对更年期综合征患者生殖内分泌-免疫功能的影响[J].中医药信息,2001,18(3):52.
[18]王文君等.雌性大鼠老化过程中脾细胞雌激素受体含量及白细胞介素-5、肿瘤坏死因子活性的变化[J].中华内分泌代谢杂志,1999,15(4):225.
[19]StefanoGB,RimuraT,Stefano JM,etal.Auto-immunomodu-21ation,age-related opioid difference dinverte bratean dinverte-brate immune systems[J].Ann.N.Y.Acad.Science,2002,663:396.
[20]Fujimoto VY,Bremenr WJ,BattagliaDE,etal.Theanterituitary response to ago nadotropin-releasing hormone hallenge testing normal order reproductive agewomen[J].Fertil Steril,1996:65:539-544.
[21]许艳丽.血清过氧化脂质水平与更年期和性别的关系,老年医学杂志,1992,12(4):15.
[22]Osen-sand,A.et al.Inhibition of Axona 1 Growth by SNAP-25 Antisense Oligonucleotides in Vitro and in Vivo[J].Nature,2003,364(29):225-448.
[23]Nagao M,Kato S.Expression of phosphotyrosine and SNAP-25immunore activity in grumose(foamy) spheroid bodies suggests axona 1 Regeneration[J].Acta Neuropathol(Beri),2004,96(4):388-394.
[24]Huang et al.Recruitment of long-lasting and protein kinase A-dependent Longterm potentiation in the CA1 region of hippocapus requires repeated tetanization Learning Memory[J].2004,35(1):74-81.
[25]Marti E,Blasi,Selective earlyinduction of synaptosoma 1 associa ted protein (molecular weight 25.000) followingsystemic administration of kainate at convulsa -nt doses in the rat.[J].Neuroscience,2001,90(4):1421-32.
[26]Mackness MI,et al.All oenzymes on paraoxonase and effectiveness of high density lipoproteins in protecting low density lipoproteinagainst lipid peroxidation Lancet.[J].2000,349(9055):851.
[27]许小凤.更年期综合征病理生理研究进展[J].医学研究通讯,2000,29(8):34-35.
[28]谈勇,许小凤,卢苏.围绝经期综合征患者ET,NO的水平与中药替代治疗的研究[J].中国医刊,2001,36(12):45.
[29]陆洪波,杨晓钊,黄永兴.南宁市妇女围绝经期综合征流行病学调查研究[J].广西医科大学学报,2001,18(5):761-763.
[30]申伟君,谭绍珍,陈柳芬.城区更年期妇女健康状况及影响因素的调查分析[J].医学文选,2001,20(5):693-695.
[31]赵更力,王临虹,渠川淡.妇女个性特征与更年期综合征初探[J].中国心理卫生杂志,1998,12(3):136-137.
[32]Netto CA,Izq uierdo I.On how passive is inhibitory avid ence[J].Neura 1 biol.2005,43(7):327-330.
[33]段涛,丰有吉主译.绝经.NOVAK妇科学[M].北京:人民卫生出版社,2005,761-782.
[34]Lien EJ.Design and discovery of new drugs by stepping-up and stepping-down Approaches.[J].Progr Drud Res,2003,40:163-189.
[35]黄薇.卵巢功能衰竭及有关疾病[M].见115-147.:王世间主编卵巢疾病.北京:人民卫生出版社,2004,115,147.
[36]陈秉枫主编.中老年妇女疾病与激素替代治疗[J].北京医科大学出版社,2002,105-106.
[37]Gusberg SB,Chen SY,Cohen CJ.Endometria 1 cancer factors influencing the choice of treatment[J].Gynecol Oncol,2004,35(2):308-313.
[38]Colditz GA,Hankinson SE,Hunter DJ.Stampfer.The use of estrogens and progestinsa nd the risk of breast cancer in postmenopa usa 1 women[J].N Engl J Med 2005,332:1589-1593.
[39]周美清,李亚里主编.现代老年妇科学[M].人民军医出版社,1999,54-64.
[1]周忠明,齐宝芳.六味地黄汤治疗更年期综合征的实验研究[J].中医杂志,2006,47(1):65.
[2]高学敏.中药学[M].北京:人民卫生出版社,2000,1315-1316.
[3]李明,李艳芳等.中药玫瑰花的研究进展[J].实验与实习卫生职业教育,2007,8(25):146-148.
[4]王蕾,王英平,等.西洋参化学成分及药理活性研究进展[J].特产研究,2007,(3):73-77.
[5]黄先菊,罗顺德.莲子心有效成分及其药理作用研究进展[J].湖北省卫生职工医学院学报,2002,15(2):481.
[6]傅晶,等.远志属植物的皂苷类成分及其药理作用研究进展[J].中草药,2006,1(37):144.
[7]杜光.枸杞子免疫药理研究及临床应用[J].湖北中医杂志,2005,27(2):54.
[8]张振明等.女贞子多糖的抗氧化活性研究[J].中国药师2005,8(6):491.
[9]袁磊.八味解郁丸治疗中风后抑郁症40例临床观察[J].吉林中医药,2008,28(2):119.
[10]毋英杰,刘建璇,宋丽晶.更年欣胶囊治疗更年期综合症的实验研究[J].中成药,1999,21(2):82-84.
[11]张红,安凤君,曹秋莲.更年欣胶囊治疗心肾不交型绝经前后诸症70例[J].长春中医学院学报,2001,17(4):32-33.
[12]杨艳,吴克明.更年归肾丸对雌性去势大鼠性激素与下丘脑神经递质调节作用的实验研究[J].世界中西医结合杂志,2007,2(8):447-450.
[13]G.J.Miller and N.E.Miler.Plasma high-density lipoprotein concentration and developement of ischaemic heart-disease[J].Lancet.1975,(1):16-18.
[10]毋英杰,刘建璇,宋丽晶.更年欣胶囊治疗更年期综合症的实验研究[J].中成药,1999,21(2):82-84.
[11]张红,安凤君,曹秋莲.更年欣胶囊治疗心肾不交型绝经前后诸症70例[J].长春中医学院学报,2001,17(4):32-33.
[12]杨艳,吴克明.更年归肾丸对雌性去势大鼠性激素与下丘脑神经递质调节作用的实验研究[J].世界中西医结合杂志,2007,2(8):447-450.
[13]Potter SM,BaumJM,Teng H,et al.Soy protein and isoflavone:their effects on blood lipids and bone density in postmenoposal women[J].Am J Clin Nutr,1998,68:1375-1379.
[14]G.J.Miller and N.E.Miler.Plasma high-density lipoprotein concentration and developement of ischaemic heart-disease[J].Lancet.1975,(1):16-18.
[15]陈友琴,胡新珉,刘鸿莲,等.云南花粉田七口服液对全血中超氧化物歧化酶活性的影响的实验研究[J].中国医学物理学杂志,1999,16(2):108-109.
[2]张涛,叶少剑等.药食两用中药复方对去卵巢大鼠血脂的调节作用[J].时珍国医国药,2005,16(9):826-827.
[3]宋.陈自明原著明.薛己校注《校注妇人良方》注释[M].第一版南昌:江西人民出版社,1983,3.
[4]明.张景岳著景岳全书[M].第一版,北京:人民卫生出版社,1991,828.
[5]王小云,沈碧琼.更年期综合征临床辨治思路[J].黑龙江中医药,2001,(2):8-9.
[6]薛静燕.健脾升清饮治疗绝经前后诸症62例[J].南京中医药大学学报,2002,18(3):186-197.
[7]余庆.脾胃阴火与更年期综合征[J].福建中医学院学报,1999,9(3):34-36.
[8]谈勇,许小凤,卢苏.绝经前后诸症心肾病机探析[J].南京中医药大学学报(自然科学版),2001,17(6):340-342.
[9]陆启滨.更年期综合征病因病机探源[J].中医药学刊,2001,19(2):139-140.
[10]李庆生.治疗妇女更年期综合征应重视柔肝疏肝.气阴双调[J].云南中医中药杂志,1997,18(3):4.
[11]叶燕萍.女性更年期综合征病机及辩证分型分型的研究[J].江苏中医,2000,211(8):18-19.
[12]夏桂成主编.围绝经期综合征.中医妇科理论与实践[M].北京:人民卫生出版社,2003,280-287.
[13]刘晓伟,邓虹蛛.更年期综合征中医辨治研究进展[J].现代中西医结合杂志,2001,10(8):792.
[14]湖波.肾虚血瘀是围绝经期综合征的重要机制[J].中医药学刊,2005,9(23):1671.
[15]王光辉,薛居宏.更年期综合征治疗八法[J].四川中医,2000,18(7):10-11.
[16]陈晓明.分型辨治更年期综合征126例[J].甘肃中医学院学报,1998,15(3):30.
[17]夏融.更年期综合征侧重治心的理论与实践[J].江苏中医,1996,17(2):41-43.
[18]陈彩云,范洪涛.导师熊铺倡治疗妇女更年期综合征经验总结[J].云南中医药杂志,2002,23(1):5
[19]王春生.更年期综合症侧重从肺论治[J].辽宁中医杂志,1989,(3):23-24.
[20]张颖,褚贵保.倪宗咖治疗更年期综合征经验[J].实用中医药杂志,2001,17(10):34.
[21]叶丽霞,严以炳,潘红玫等.中医治疗更年期综合征86例疗效观察[J].长春中医学院学报,2001,17(3):35.
[22]张华,张淑芳,李国鹏.中西医结合治疗妇女更年期综合征206例[J].陕西中医,2001,22(11):659.
[23]张立.妇女更年期综合征的辨证分型与治疗[J].中医药学刊,2002,20(4):534.
[24]周焕凤,白文成.从肝肾论治妇女更年期综合征36例[J].陕西中医,2001,22(11):658.
[25]李得萍.更年期综合征辨治体会[J].实用中医药杂志,2002,9(18-9):34-35.
[26]李莉,孙竹春.左归丸治疗妇女更年期综合征72例[J].上海中医药杂志,2001,35(3):26-27.
[27]舒晓春等.龙风宝胶囊治疗更年期综合征50例[J].陕西中医,2003,23(8):715.
[28]李琼,舒仪琼,赖慧红等.镇肝熄风汤治疗围绝经期综合征的临床疗效观察[J].中国中西医结合杂志(英文版),2003,9(1):63-66.
[29]田晓娥.二仙汤加味治疗围绝经期综合征[J].Journal of Medical Forum,2004,25(2):57.
[30]王书杰,王丽萍,李丽荣.佳蓉片治疗更年期综合征疗效观察[J].时珍国医国药,2003,14(9):549.
[31]龚瑾,黄家茹.血府逐瘀胶囊治疗更年期综合征106例临床观察[J].Beijing JournalofTCM,2004,23(2):128.
[32]蔡雪芬等.更年宁心胶囊治疗更年期综合征65例[J].中国中西医结合杂志,2003,23(11):875.
[33]李国安,俞吟.针刺治疗女性更年期综合征的临床研究[J].上海针灸杂志,2002,21(3):6-8.
[34]孙敬青.走罐疗法治疗更年期综合征50例临床观察[J].针灸临床杂志,1999,15(7):31-32.
[35]孙冬梅,吴富东,单秋华,王金玲.耳穴贴压治疗女性更年期综合征的临床观察[J].针灸临床杂志,2003,19(12):32-35.
[36]潘红玲,许天兵.穴位注射治疗更年期综合征临床观察[J].针灸临床观察,1998,14(11):14.
[37]黎烈荣.更年期综合征患者情绪异常如何治疗[J].中医杂志,1998,39(1):54.
[1]徐苓等.围绝经期及绝经过渡期的定义及激素补充治疗[J].实用妇产科杂志,1999,15(4):177-178.
[2]卫生部继续教育委员会编.女性生殖内分泌性激素补充疗法[M].北京:中国协和医科大学出版社,1999.
[3]顾美皎.现代妇产科学[M].人民军医出版社,2002,1222.
[4]刘敏如,吴克明.世界传统医妇科学[M].科学技术出版社,1999,235.
[5]郎景和,向阳主编.高级医师案头丛书妇产科学[M].北京:中国协和医科大学出版社,2001:340.
[6]邓小虹,张松文.北京地区更年期妇女健康状况的流行病学调查[J].北京医学.2002,24(4):235-238:13.
[7]Fujimoto VY,Bremenr WJ,Battaglia DE,et al.The anterior pituitary response toagonadotropin-releasing hormone challenge test in normal older reproductive-age women[J].Fertil Steri,1996,6:539.
[8]步世忠,等.更年健上调老年雌性大鼠下丘脑雌激素受体mRNA表达对P物质和β-内啡肽的影响[J].中国中西医结合杂志,1998,18(1):28-30.
[9]范红霞,万力生,黎烈荣.更年安怡片对去卵巢大鼠脑组织中单胺类神经递质含量的影响[J].中医研究,1999,12(6):11-13.
[10]胡随瑜,尤劲松,向群辉等.地贞颗粒对“更年期”拟阴虚内热证模型大鼠的影响[J].中药新药与临床药理,2000,11(3):143-145.
[11]Brown ER Harlan RE,Krause LE.Gomadal steroi dregula 2tiono facidsin theratan terior pituitary and hypothalamus[J].Endocrinology,1990,126:330-340.
[12]吕肖锋,叶雪清.绝经后妇女血β-内啡肽及其抗体改变[J].中国实验临床免疫学杂志,1993,5(4):37.
[13]王跃清等.β-内啡肽对更年期妇女潮热的影响[J].实用妇产科杂志,1999,15(6):321.
[14]江仙远,陈友香,侯安继.更年康片治疗围绝经期综合征的机理研究[J].中药药理与临床,2001,17(1):36-38.
[15]王大增,朱燕青,李燕萍等.更年舒治疗妇女更年期综合征的临床及实验研究[J].中国中西医结合杂志,1994,14(7):396-399.
[16]HirikawaK,UtsuyamaM,KasiM,etal.Aging and immunocity[J].Acta Pathopn,2002,42(8):537-540.
[17]张雅萍,王秀霞.坤宁安丸对更年期综合征患者生殖内分泌-免疫功能的影响[J].中医药信息,2001,18(3):52.
[18]王文君等.雌性大鼠老化过程中脾细胞雌激素受体含量及白细胞介素-5、肿瘤坏死因子活性的变化[J].中华内分泌代谢杂志,1999,15(4):225.
[19]StefanoGB,RimuraT,Stefano JM,etal.Auto-immunomodu-21ation,age-related opioid difference dinverte bratean dinverte-brate immune systems[J].Ann.N.Y.Acad.Science,2002,663:396.
[20]Fujimoto VY,Bremenr WJ,BattagliaDE,etal.Theanterituitary response to ago nadotropin-releasing hormone hallenge testing normal order reproductive agewomen[J].Fertil Steril,1996:65:539-544.
[21]许艳丽.血清过氧化脂质水平与更年期和性别的关系,老年医学杂志,1992,12(4):15.
[22]Osen-sand,A.et al.Inhibition of Axona 1 Growth by SNAP-25 Antisense Oligonucleotides in Vitro and in Vivo[J].Nature,2003,364(29):225-448.
[23]Nagao M,Kato S.Expression of phosphotyrosine and SNAP-25immunore activity in grumose(foamy) spheroid bodies suggests axona 1 Regeneration[J].Acta Neuropathol(Beri),2004,96(4):388-394.
[24]Huang et al.Recruitment of long-lasting and protein kinase A-dependent Longterm potentiation in the CA1 region of hippocapus requires repeated tetanization Learning Memory[J].2004,35(1):74-81.
[25]Marti E,Blasi,Selective earlyinduction of synaptosoma 1 associa ted protein (molecular weight 25.000) followingsystemic administration of kainate at convulsa -nt doses in the rat.[J].Neuroscience,2001,90(4):1421-32.
[26]Mackness MI,et al.All oenzymes on paraoxonase and effectiveness of high density lipoproteins in protecting low density lipoproteinagainst lipid peroxidation Lancet.[J].2000,349(9055):851.
[27]许小凤.更年期综合征病理生理研究进展[J].医学研究通讯,2000,29(8):34-35.
[28]谈勇,许小凤,卢苏.围绝经期综合征患者ET,NO的水平与中药替代治疗的研究[J].中国医刊,2001,36(12):45.
[29]陆洪波,杨晓钊,黄永兴.南宁市妇女围绝经期综合征流行病学调查研究[J].广西医科大学学报,2001,18(5):761-763.
[30]申伟君,谭绍珍,陈柳芬.城区更年期妇女健康状况及影响因素的调查分析[J].医学文选,2001,20(5):693-695.
[31]赵更力,王临虹,渠川淡.妇女个性特征与更年期综合征初探[J].中国心理卫生杂志,1998,12(3):136-137.
[32]Netto CA,Izq uierdo I.On how passive is inhibitory avid ence[J].Neura 1 biol.2005,43(7):327-330.
[33]段涛,丰有吉主译.绝经.NOVAK妇科学[M].北京:人民卫生出版社,2005,761-782.
[34]Lien EJ.Design and discovery of new drugs by stepping-up and stepping-down Approaches.[J].Progr Drud Res,2003,40:163-189.
[35]黄薇.卵巢功能衰竭及有关疾病[M].见115-147.:王世间主编卵巢疾病.北京:人民卫生出版社,2004,115,147.
[36]陈秉枫主编.中老年妇女疾病与激素替代治疗[J].北京医科大学出版社,2002,105-106.
[37]Gusberg SB,Chen SY,Cohen CJ.Endometria 1 cancer factors influencing the choice of treatment[J].Gynecol Oncol,2004,35(2):308-313.
[38]Colditz GA,Hankinson SE,Hunter DJ.Stampfer.The use of estrogens and progestinsa nd the risk of breast cancer in postmenopa usa 1 women[J].N Engl J Med 2005,332:1589-1593.
[39]周美清,李亚里主编.现代老年妇科学[M].人民军医出版社,1999,54-64.
[1]周忠明,齐宝芳.六味地黄汤治疗更年期综合征的实验研究[J].中医杂志,2006,47(1):65.
[2]高学敏.中药学[M].北京:人民卫生出版社,2000,1315-1316.
[3]李明,李艳芳等.中药玫瑰花的研究进展[J].实验与实习卫生职业教育,2007,8(25):146-148.
[4]王蕾,王英平,等.西洋参化学成分及药理活性研究进展[J].特产研究,2007,(3):73-77.
[5]黄先菊,罗顺德.莲子心有效成分及其药理作用研究进展[J].湖北省卫生职工医学院学报,2002,15(2):481.
[6]傅晶,等.远志属植物的皂苷类成分及其药理作用研究进展[J].中草药,2006,1(37):144.
[7]杜光.枸杞子免疫药理研究及临床应用[J].湖北中医杂志,2005,27(2):54.
[8]张振明等.女贞子多糖的抗氧化活性研究[J].中国药师2005,8(6):491.
[9]袁磊.八味解郁丸治疗中风后抑郁症40例临床观察[J].吉林中医药,2008,28(2):119.
[10]毋英杰,刘建璇,宋丽晶.更年欣胶囊治疗更年期综合症的实验研究[J].中成药,1999,21(2):82-84.
[11]张红,安凤君,曹秋莲.更年欣胶囊治疗心肾不交型绝经前后诸症70例[J].长春中医学院学报,2001,17(4):32-33.
[12]杨艳,吴克明.更年归肾丸对雌性去势大鼠性激素与下丘脑神经递质调节作用的实验研究[J].世界中西医结合杂志,2007,2(8):447-450.
[13]G.J.Miller and N.E.Miler.Plasma high-density lipoprotein concentration and developement of ischaemic heart-disease[J].Lancet.1975,(1):16-18.
[10]毋英杰,刘建璇,宋丽晶.更年欣胶囊治疗更年期综合症的实验研究[J].中成药,1999,21(2):82-84.
[11]张红,安凤君,曹秋莲.更年欣胶囊治疗心肾不交型绝经前后诸症70例[J].长春中医学院学报,2001,17(4):32-33.
[12]杨艳,吴克明.更年归肾丸对雌性去势大鼠性激素与下丘脑神经递质调节作用的实验研究[J].世界中西医结合杂志,2007,2(8):447-450.
[13]Potter SM,BaumJM,Teng H,et al.Soy protein and isoflavone:their effects on blood lipids and bone density in postmenoposal women[J].Am J Clin Nutr,1998,68:1375-1379.
[14]G.J.Miller and N.E.Miler.Plasma high-density lipoprotein concentration and developement of ischaemic heart-disease[J].Lancet.1975,(1):16-18.
[15]陈友琴,胡新珉,刘鸿莲,等.云南花粉田七口服液对全血中超氧化物歧化酶活性的影响的实验研究[J].中国医学物理学杂志,1999,16(2):108-109.