Wnt-1与分泌性卷曲相关蛋白-1（Sfrp-1）在病理性瘢痕中的表达及意义
摘要
目的:研究病理性瘢痕组织中Wnt-1与Sfrp-1的表达及其临床意义
方法:采用链霉素亲生物素-过氧化酶免疫组织化学方法(SP法)检测2006年3月至2007年3月中南大学湘雅二医院烧伤整形科的瘢痕标本48例(其中增生性瘢痕28例、瘢痕疙瘩8例、外科瘢痕12例)和正常皮肤标本8例(患者和供皮者均知情同意)中Wnt-1与Sfrp-1的表达,采用着色强度和阳性细胞率二者评分相结合的方法观察分析:1 Wnt-1和Sfrp-1在增生性瘢痕,瘢痕疙瘩,外科瘢痕,正常皮肤组织中的表达差异;2 Wnt-1和Sfrp-1表达的相关性。
结果:
1增生性瘢痕、瘢痕疙瘩中Wnt-1的表达评分值和阳性率高于外科瘢痕、正常皮肤组织,P＜0.05,两者差异有统计学意义;增生性瘢痕与瘢痕疙瘩,外科瘢痕与正常皮肤组织比较,P＞0.05,两者差异无显著意义。
2外科瘢痕、正常皮肤组织中Sfrp-1的表达评分值和阳性率高于增生性瘢痕、瘢痕疙瘩,P＜0.05,两者差异有统计学意义;增生性瘢痕与瘢痕疙瘩,外科瘢痕与正常皮肤组织比较,P＞0.05,两者差异无显著意义。
3 Wnt-1、Sfrp-1在增生性瘢痕、瘢痕疙瘩、外科瘢痕、正常皮肤中的表达呈负相关(相关系数r=-0.373)。
结论:
1 Wnt-1在增生性瘢痕、瘢痕疙瘩中的高表达提示Wnt-1可能在病理性瘢痕的形成中起促进作用。
2 Sfrp-1在外科瘢痕和正常皮肤中高表达,在增生性瘢痕、瘢痕疙瘩中的低表达提示Sfrp-1可能在病理性瘢痕中起抑制作用。
3干预Wnt-1和Sfrp-1的表达可能为防治病理性瘢痕提供有效的手段。
Objective:To study the expressions of Wnt-1 and Sfrp-1 in hypertrophic scar,keloid,surgical scar and normal skin tissues,and investigate their roles and significances in pathological scar.
Methods:All these 48 patient scars(28 hypertrophic scars,8 keloids and 12 surgical scars)and 8 normal skin tissues were all originated from Department of Bums and Plastic Surgery,Xiangya Second Hospital,Central South University from March 2006 to March 2007(The recipients and donors all signed the informed consent.).They were stained by streptoavidin-biotion method of immunohistochemistry. Expressions of polyclonal antibody against Wnt-1 and Sfrp-1 and their correlation were determined.The scores of evaluation depended mainly on the straining degree and the positive cell counts of the sections.
RESULTS:①The scores of Wnt-1 in the hypertrophic scar and the keloid were higher than those in the surgical scar and the normal skin (p＜0.05).No significant difference was found between hypertrophic scar and the keloid,between surgical scar and the normal skin(p＞0.05). ②The scores of Sfrp-1 in the surgical scar and the normal skin were higher than those in the hypertrophic scar and the keloid(p＜0.05).No significant difference was found between hypertrophic scar and the keloid,between surgical scar and the normal skin(p＞0.05).③The expression of Sfrp-1 was negatively correlated with wnt-1 in hypertrophic scar,keloid,surgical scar and normal skin.(r=-0.373,p＜0.05).
CONCLUSION:
1 The overexpression of Wnt-1 in hypertrophic scar and keloid means the Wnt-1 may stimulate the formation of pathological scar.
2 The overexpressions of Sfrp-1 in surgical scar and normal skin, which expression is low in hypertrophic scar and keloid,means the Sfrp-1 may inhibit the formation of pathological scar.
3 Intervening the expression of Wnt-1 and Sfrp-1 may provide an effective method for clinical prevention and treatment of pathological scar.
方法:采用链霉素亲生物素-过氧化酶免疫组织化学方法(SP法)检测2006年3月至2007年3月中南大学湘雅二医院烧伤整形科的瘢痕标本48例(其中增生性瘢痕28例、瘢痕疙瘩8例、外科瘢痕12例)和正常皮肤标本8例(患者和供皮者均知情同意)中Wnt-1与Sfrp-1的表达,采用着色强度和阳性细胞率二者评分相结合的方法观察分析:1 Wnt-1和Sfrp-1在增生性瘢痕,瘢痕疙瘩,外科瘢痕,正常皮肤组织中的表达差异;2 Wnt-1和Sfrp-1表达的相关性。
结果:
1增生性瘢痕、瘢痕疙瘩中Wnt-1的表达评分值和阳性率高于外科瘢痕、正常皮肤组织,P＜0.05,两者差异有统计学意义;增生性瘢痕与瘢痕疙瘩,外科瘢痕与正常皮肤组织比较,P＞0.05,两者差异无显著意义。
2外科瘢痕、正常皮肤组织中Sfrp-1的表达评分值和阳性率高于增生性瘢痕、瘢痕疙瘩,P＜0.05,两者差异有统计学意义;增生性瘢痕与瘢痕疙瘩,外科瘢痕与正常皮肤组织比较,P＞0.05,两者差异无显著意义。
3 Wnt-1、Sfrp-1在增生性瘢痕、瘢痕疙瘩、外科瘢痕、正常皮肤中的表达呈负相关(相关系数r=-0.373)。
结论:
1 Wnt-1在增生性瘢痕、瘢痕疙瘩中的高表达提示Wnt-1可能在病理性瘢痕的形成中起促进作用。
2 Sfrp-1在外科瘢痕和正常皮肤中高表达,在增生性瘢痕、瘢痕疙瘩中的低表达提示Sfrp-1可能在病理性瘢痕中起抑制作用。
3干预Wnt-1和Sfrp-1的表达可能为防治病理性瘢痕提供有效的手段。
Objective:To study the expressions of Wnt-1 and Sfrp-1 in hypertrophic scar,keloid,surgical scar and normal skin tissues,and investigate their roles and significances in pathological scar.
Methods:All these 48 patient scars(28 hypertrophic scars,8 keloids and 12 surgical scars)and 8 normal skin tissues were all originated from Department of Bums and Plastic Surgery,Xiangya Second Hospital,Central South University from March 2006 to March 2007(The recipients and donors all signed the informed consent.).They were stained by streptoavidin-biotion method of immunohistochemistry. Expressions of polyclonal antibody against Wnt-1 and Sfrp-1 and their correlation were determined.The scores of evaluation depended mainly on the straining degree and the positive cell counts of the sections.
RESULTS:①The scores of Wnt-1 in the hypertrophic scar and the keloid were higher than those in the surgical scar and the normal skin (p＜0.05).No significant difference was found between hypertrophic scar and the keloid,between surgical scar and the normal skin(p＞0.05). ②The scores of Sfrp-1 in the surgical scar and the normal skin were higher than those in the hypertrophic scar and the keloid(p＜0.05).No significant difference was found between hypertrophic scar and the keloid,between surgical scar and the normal skin(p＞0.05).③The expression of Sfrp-1 was negatively correlated with wnt-1 in hypertrophic scar,keloid,surgical scar and normal skin.(r=-0.373,p＜0.05).
CONCLUSION:
1 The overexpression of Wnt-1 in hypertrophic scar and keloid means the Wnt-1 may stimulate the formation of pathological scar.
2 The overexpressions of Sfrp-1 in surgical scar and normal skin, which expression is low in hypertrophic scar and keloid,means the Sfrp-1 may inhibit the formation of pathological scar.
3 Intervening the expression of Wnt-1 and Sfrp-1 may provide an effective method for clinical prevention and treatment of pathological scar.
引文
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