共同性外斜视内直肌Myf-5表达研究
摘要
目的观察共同性外斜视患者内直肌中Myf-5的表达变化,探讨其在共同性外斜视发病机制中的作用。
方法收集青岛大学医学院附属医院2009年12月-2010年11月住院手术治疗的共同性外斜视病人获得的内直肌段为斜视组,共16例。斜视组又根据年龄分为儿童斜视组(8例)和成人斜视组(8例)。另选同期行眼球摘除或角膜移植供体取得的内直肌为成人对照组(共6例)。所有的内直肌段固定切片后行Myf-5免疫组织化学染色,测Myf-5在眼外肌中表达的平均光密度值。对各组间平均光密度值进行比较,并对斜视患者眼外肌中Myf-5平均光密度值与斜视度及年龄进行Pearson相关性分析。
结果①Mv.f-5的平均光密度值在斜视组0.024±0.041,成人对照组0.233±0.024,两者差异具有统计学意义(P<0.01)：在儿童斜视组0.156±0.011,成人斜视组0.095±0.038,两者差异具有统计学意义(P<0.01);成人对照组0.233±0.024,成人斜视组0.095±0.038,两者差异具有统计学意义(P<0.01)。②斜视患者Myf-5表达与患者的斜视度和年龄呈负相关(r=-0.728,P=0.001,r=-0.643,P=0.004)。
结论眼外肌中Myf-5表达的降低可能与斜视的发病有关,眼外肌中.Myf-5的表达变化可能与斜视的发展有关。
Objective To observe the expression of Myf-5 in extra ocular muscle (EOMs) of patients with concomitant extropia. To investigate the role of Myf-5 in the etiology of concomitant extropia.
Methods We collected 16 cases patients with concomitant extropia from December 2009 to November 2010 in Affiliated Hospital of Medical College of Qingdao University,and then divided them into two groups:the group of children with concomitant extropia and the group of adults with concomitant extropia. Medial rectus muscles were got with agreement of patients accepted surgical resection. Control muscles were obtained from patients with eyeballs removed with injury or corneal transplant donors. Cross sections were stained immunochemically and measured the mean optical density according to the expression of Myf-5. To compare the difference in different groups and analyze the relationship among the expression of Myf-5,the age and the strabismus degree.
Results①There were significant differences(P< 0.01)between the control group (0.233±0.024)and the strabismus group(0.024±0.041),the group of children with concomitant extropia(0.156±0.011) and the group of adults with concomitant extropia(0.095±0.038),the control group (0.233±0.024)and the group of adults with concomitant extropia(0.095±0.038). The formers had higher levels than the latters.±There were negative relationships between Myf-5 expression and patients' age and their strabismus degrees.
Conclusion The decreased level of Myf-5expression may participate in the incidence of concomitant extropia. The changement of the expression of Myf-5 in extraocular muscles may result in deteriorating of concomitant extropia.
方法收集青岛大学医学院附属医院2009年12月-2010年11月住院手术治疗的共同性外斜视病人获得的内直肌段为斜视组,共16例。斜视组又根据年龄分为儿童斜视组(8例)和成人斜视组(8例)。另选同期行眼球摘除或角膜移植供体取得的内直肌为成人对照组(共6例)。所有的内直肌段固定切片后行Myf-5免疫组织化学染色,测Myf-5在眼外肌中表达的平均光密度值。对各组间平均光密度值进行比较,并对斜视患者眼外肌中Myf-5平均光密度值与斜视度及年龄进行Pearson相关性分析。
结果①Mv.f-5的平均光密度值在斜视组0.024±0.041,成人对照组0.233±0.024,两者差异具有统计学意义(P<0.01)：在儿童斜视组0.156±0.011,成人斜视组0.095±0.038,两者差异具有统计学意义(P<0.01);成人对照组0.233±0.024,成人斜视组0.095±0.038,两者差异具有统计学意义(P<0.01)。②斜视患者Myf-5表达与患者的斜视度和年龄呈负相关(r=-0.728,P=0.001,r=-0.643,P=0.004)。
结论眼外肌中Myf-5表达的降低可能与斜视的发病有关,眼外肌中.Myf-5的表达变化可能与斜视的发展有关。
Objective To observe the expression of Myf-5 in extra ocular muscle (EOMs) of patients with concomitant extropia. To investigate the role of Myf-5 in the etiology of concomitant extropia.
Methods We collected 16 cases patients with concomitant extropia from December 2009 to November 2010 in Affiliated Hospital of Medical College of Qingdao University,and then divided them into two groups:the group of children with concomitant extropia and the group of adults with concomitant extropia. Medial rectus muscles were got with agreement of patients accepted surgical resection. Control muscles were obtained from patients with eyeballs removed with injury or corneal transplant donors. Cross sections were stained immunochemically and measured the mean optical density according to the expression of Myf-5. To compare the difference in different groups and analyze the relationship among the expression of Myf-5,the age and the strabismus degree.
Results①There were significant differences(P< 0.01)between the control group (0.233±0.024)and the strabismus group(0.024±0.041),the group of children with concomitant extropia(0.156±0.011) and the group of adults with concomitant extropia(0.095±0.038),the control group (0.233±0.024)and the group of adults with concomitant extropia(0.095±0.038). The formers had higher levels than the latters.±There were negative relationships between Myf-5 expression and patients' age and their strabismus degrees.
Conclusion The decreased level of Myf-5expression may participate in the incidence of concomitant extropia. The changement of the expression of Myf-5 in extraocular muscles may result in deteriorating of concomitant extropia.
引文
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