欧洲葡萄ERFs基因启动子序列分析比较
摘要
欧洲葡萄(Vitis vinifera L.)是葡萄亚属中三大种群之一,也是世界各国主要的栽培品种,因此关于欧洲葡萄的研究报道较之其它种属葡萄更多更广,同时也说明了欧洲葡萄在葡萄属植物中的重要地位。可是目前对欧洲葡萄ERF基因的研究还不是很热门,对其启动子的研究报道更是少之又少,而植物启动子的研究对于基因的转录调控、植物的品种改良、作物的抗逆能力的增强等方面尤为重要,所以笔者认为,针对欧洲葡萄ERF基因启动子进行研究是必不可少的。
本文从欧洲葡萄的ERF基因着手,首先利用本实验室保存的8个欧洲葡萄ERF基因的ORF序列,通过氨基酸的分析比较,得出了一个相同的三级结构立体图形,说明了它们确实隶属于同一基因家族,而后用MEGA 3.1作出关于它们相似度的进化树,再运用生物信息学的方法预测出了此8个同一家族基因的启动子序列,并重点对欧洲葡萄的8个ERF基因启动子的顺式作用元件进行了分析,可以看出在同一基因家族中基因的启动子之间的相似之处与不同之处,阐明了这些启动子较为关键的顺式作用元件在基因转录过程中的作用。
另外,根据欧洲葡萄8个ERF基因的氨基酸序列,Blastp后得到了同缘性较高8个拟南芥(Arabidopsis)AtERF基因,并将它们一一对应,从相同顺式元件的数量、位置、功能等方面进行比较分析,可以看出相似基因的启动子区域在不同物种之间的差异,从研究较为清楚的模式生物——拟南芥中预测出了欧洲葡萄ERF基因启动子的更多功能。
Vitis vinifera L. is one of the three grape subgenus and it is a primary cultivar to every country in the world,therefore the research about Vitis vinifera L. is more than the studies about the other categories. Simultaneity,it is illuminated that Vitis vinifera L is one of the most important species in grape subgenus. At present,ERF gene of Vitis vinifera L’s research isn’t a pop one,studies for it’s promoters are very few. Plant promoter is very important in gene transcription,breed improving and enhancing ability to resist adversity. So penman consider that it is absolutely necessarily to the study in ERF gene of Vitis vinifera L.
This text commence on ERF gene of Vitis vinifera L. In the first place,using ORF sequences of eight ERF gene of Vitis vinifera L,compare their amino acid,we will get a same protein tertiary structure,it is said that they belong to the same gene family, then work out their phylogenetic analysis with the software MEGA 3.1,and forecast the sequences of eight ERF gene’s promoters with bioinformatics,later,analyze the cis-acting element of eight ERF gene promoters. We will know the similitude and difference in their promoter sequences which are in the same gene family. Finally,we illuminate the function of some important cis-acting element in gene transcription.
Otherwise,according to the amino acid sequences of Vitis vinifera L. ERF gene,we can acquire eight AtERF gene in Arabidopsis with the method“Blastp”,and make them homologous one by one,analyze number,position,function of their cis-acting element,we may find out the difference in this two species which provide the region of ERF gene promoter. Then we can forecast more function in Vitis vinifera L. ERF gene promoters from mode plant—Arabidopsis.
本文从欧洲葡萄的ERF基因着手,首先利用本实验室保存的8个欧洲葡萄ERF基因的ORF序列,通过氨基酸的分析比较,得出了一个相同的三级结构立体图形,说明了它们确实隶属于同一基因家族,而后用MEGA 3.1作出关于它们相似度的进化树,再运用生物信息学的方法预测出了此8个同一家族基因的启动子序列,并重点对欧洲葡萄的8个ERF基因启动子的顺式作用元件进行了分析,可以看出在同一基因家族中基因的启动子之间的相似之处与不同之处,阐明了这些启动子较为关键的顺式作用元件在基因转录过程中的作用。
另外,根据欧洲葡萄8个ERF基因的氨基酸序列,Blastp后得到了同缘性较高8个拟南芥(Arabidopsis)AtERF基因,并将它们一一对应,从相同顺式元件的数量、位置、功能等方面进行比较分析,可以看出相似基因的启动子区域在不同物种之间的差异,从研究较为清楚的模式生物——拟南芥中预测出了欧洲葡萄ERF基因启动子的更多功能。
Vitis vinifera L. is one of the three grape subgenus and it is a primary cultivar to every country in the world,therefore the research about Vitis vinifera L. is more than the studies about the other categories. Simultaneity,it is illuminated that Vitis vinifera L is one of the most important species in grape subgenus. At present,ERF gene of Vitis vinifera L’s research isn’t a pop one,studies for it’s promoters are very few. Plant promoter is very important in gene transcription,breed improving and enhancing ability to resist adversity. So penman consider that it is absolutely necessarily to the study in ERF gene of Vitis vinifera L.
This text commence on ERF gene of Vitis vinifera L. In the first place,using ORF sequences of eight ERF gene of Vitis vinifera L,compare their amino acid,we will get a same protein tertiary structure,it is said that they belong to the same gene family, then work out their phylogenetic analysis with the software MEGA 3.1,and forecast the sequences of eight ERF gene’s promoters with bioinformatics,later,analyze the cis-acting element of eight ERF gene promoters. We will know the similitude and difference in their promoter sequences which are in the same gene family. Finally,we illuminate the function of some important cis-acting element in gene transcription.
Otherwise,according to the amino acid sequences of Vitis vinifera L. ERF gene,we can acquire eight AtERF gene in Arabidopsis with the method“Blastp”,and make them homologous one by one,analyze number,position,function of their cis-acting element,we may find out the difference in this two species which provide the region of ERF gene promoter. Then we can forecast more function in Vitis vinifera L. ERF gene promoters from mode plant—Arabidopsis.
引文
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[9]刘武,戴良英.植物抗逆相关ERF转录因子研究综述.农业生物技术科学.2007(4):33-37.
[10]Jose Luis Riechmann,Elliot M Meyerowitz.The ERF family of plant transcription factors.BiolChem.(1998),379:633-646.
[11]Han Zhiping,An Lijia,Hou Hesheng.The Structure and Function of AP2/EREBP Transcription Factors.Chinajournal,(2006),22(2): 33-38.
[12]HaoD,Ohme TakagiM,SaraiA. Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant. JBiolChem, (1998),273:26857-26861.
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