细胞粘附、影响因素及粘附分子基因表达的调控
摘要
白细胞与血小板、内皮细胞及平滑肌细胞的粘附是多种急、慢性心血管疾病发生、发展的重要环节。这些心血管疾病包括动脉粥样硬化、血栓形成、缺血再灌损伤、经皮腔内血管成形术后血管再狭窄等。抑制病理状态下细胞与细胞间的相互粘附能有效地阻止或降低急、慢性炎症引起的组织损伤，预防上述疾病的发生与发展。已知许多因素影响细胞间的粘附，对这些影响因素，特别是药物的研究，不论在基础理论还是在临床实践方面都有十分重要的意义。
正常人或动物的白细胞与血小板的粘附率很低，但急性心肌梗塞病人和动物病理模型的白细胞与血小板的粘附率则显著增加。研究结果表明：白细胞与血小板的激活是导致这两种细胞相互粘附的原因。凝血酶激活的血小板与白细胞的粘附率也显著增加。白细胞与血小板的粘附是钙依赖性的，肝素、硫酸右旋糖苷可抑制心肌梗塞大鼠白细胞与血小板的粘附，而不含硫酸根的右旋糖苷则无抑制作用。提示GMP—140至少部分介导白细胞与血小板的粘附。急性心肌梗塞病人的白细胞CD11a、CD11b的表达明显高于正常人，表明粘附分子CD11a／CD18及CD11b／CD18可能参与白细胞与血小板的粘附。槲皮素在心肌梗塞形成前静脉给药，或在血小板被凝血酶激活之前体外给药均可抑制白细胞与血小板的粘附。阿司匹林也有类似的抑制作用。
本文结果显示，未激活的白细胞可抑制血小板聚集，阿司匹林及槲皮素均能加强白细胞对血小板激活的抑制作用。但当白细胞被PMA激活后，其抑制血小板聚集的作用则消失，如在PMA激活白细胞前加槲皮素与血小板孵育，白细胞对血小板聚集的抑制作用又可恢复，提示槲皮素可抑制PMA对白细胞的激活。
TNF_α激活的内皮细胞与白细胞粘附呈量效和时效关系。与内皮细胞粘附的单核细胞较中性粒细胞略多，且随刺激时间的延长作用更为明显。抗ICAM-1，E-selectin及VCAM-1单克隆抗体抑制白细胞与内皮细胞粘附，提示这些粘附分子介导上述细胞粘附。ELISA法及流式细胞术显示，静息状态的内皮细胞表面有少量的ICAM-1及VCAM-1表达，无E-selectin表达，但RT-PCR法却显示内皮细胞内含有这三种粘附分子的mRNA。TNF_α诱导／增加ICAM-1，E-selectin及VCAM-1的表达也呈量效和时效关系。然而，它们的时效曲线却各具特点。E-selectin起效最快，达峰时间最短，为4～6小时，12小时已基本消退。ICAM-1表达较E-selectin慢，2小时略有增加，12小时达高峰，24小时
Adhesion of leukocyte to platelet, endothelial cell (EC) and vascular smooth muscle cell (SMC), is an essential component of various acute and chronic cardiovascular diseases including atherosclerosis, thrombosis, reperfusion injury and restenosis after percutaneous transluminal coronary angioplasty etc. Inhibition of cells adhesion of pathological status is effective in reducing the tissue injury induced by acute or chronic inflammations and in preventing the genesis and progress of the diseases mentioned above. It is known that several factors influence the cells adhesion. To study these regulating factors, especially drugs, may be of considerable importance for basic theory and clinical application.
The adhesion of leukocyte/platelet was very low in healthy volunteers and normal animals. It was increased dramatically in patients with acute myocardial ischaemia (AMI) or experimental pathological animal models. The results indicated that the activation of both leukocyte and platelet played an important role in increasing both cells adhesion in AMI. The leukocyte/platelet adhesion rate was also increased when platelets were stimulated by thrombin in vitro. The adhesion of leukocyte/platelet was Ca~(2+) dependent, heparin or dextran sulfate inhibited both cells binding but not dextran. This indicates that adhesion molecule—GMP-140 might be involved in the mechanism of leukocyte/platelet adhesion. In addition, the expression of CDlla and CD 11b on the surface of leukocyte in AMI patients was significantly increased than normal subjects. It suggests that the adhesion molecules— CD11a/CD18 and CD11b/CD18 may mediate leukocyte/platelet adhesioa
Quercetin inhibited the leukocyte/platelet adhesion in AMI animals when administered intravenously before the formation of AMI model or before the platelet stimulated by thrombin. Aspirin had similar effects as quercetin.
The results showed that unactivated leukocyte inhibited platelets aggregation. Both quercetin and aspirin enhanced the inhibitory effects of leukocyte on platelet activation. However, the inhibition of platelet aggregation was disappeared when leukocyte was activated by PMA. It is interested to find out that if incubation of quercetin with platelets before activation of leukocyte by PMA, the inhibitory effect of leukocyte on platelet aggregation was restored. It indicates that quercetin has the ability to prevent the activation of leukocyte by PMA.
TNF_α increased the adhesion of EC to leukocyte both time- and dose-dependently. More monocytes bound to EC than neutrophils, and it became more significant with the prolongation of stimulation time. Anti-ICAM-1, anti-E-selectin and anti-VCAM-1 monoclonal antibodies blocked the binding of TNF_α stimulated EC to leukocyte, suggesting that the adhesion molecules, ICAM-1, E-selectin and VCAM-1, mediated both cells adhesion.
ELISA and flow cytometric analysis revealed that the expression of ICAM-1 and
正常人或动物的白细胞与血小板的粘附率很低，但急性心肌梗塞病人和动物病理模型的白细胞与血小板的粘附率则显著增加。研究结果表明：白细胞与血小板的激活是导致这两种细胞相互粘附的原因。凝血酶激活的血小板与白细胞的粘附率也显著增加。白细胞与血小板的粘附是钙依赖性的，肝素、硫酸右旋糖苷可抑制心肌梗塞大鼠白细胞与血小板的粘附，而不含硫酸根的右旋糖苷则无抑制作用。提示GMP—140至少部分介导白细胞与血小板的粘附。急性心肌梗塞病人的白细胞CD11a、CD11b的表达明显高于正常人，表明粘附分子CD11a／CD18及CD11b／CD18可能参与白细胞与血小板的粘附。槲皮素在心肌梗塞形成前静脉给药，或在血小板被凝血酶激活之前体外给药均可抑制白细胞与血小板的粘附。阿司匹林也有类似的抑制作用。
本文结果显示，未激活的白细胞可抑制血小板聚集，阿司匹林及槲皮素均能加强白细胞对血小板激活的抑制作用。但当白细胞被PMA激活后，其抑制血小板聚集的作用则消失，如在PMA激活白细胞前加槲皮素与血小板孵育，白细胞对血小板聚集的抑制作用又可恢复，提示槲皮素可抑制PMA对白细胞的激活。
TNF_α激活的内皮细胞与白细胞粘附呈量效和时效关系。与内皮细胞粘附的单核细胞较中性粒细胞略多，且随刺激时间的延长作用更为明显。抗ICAM-1，E-selectin及VCAM-1单克隆抗体抑制白细胞与内皮细胞粘附，提示这些粘附分子介导上述细胞粘附。ELISA法及流式细胞术显示，静息状态的内皮细胞表面有少量的ICAM-1及VCAM-1表达，无E-selectin表达，但RT-PCR法却显示内皮细胞内含有这三种粘附分子的mRNA。TNF_α诱导／增加ICAM-1，E-selectin及VCAM-1的表达也呈量效和时效关系。然而，它们的时效曲线却各具特点。E-selectin起效最快，达峰时间最短，为4～6小时，12小时已基本消退。ICAM-1表达较E-selectin慢，2小时略有增加，12小时达高峰，24小时
Adhesion of leukocyte to platelet, endothelial cell (EC) and vascular smooth muscle cell (SMC), is an essential component of various acute and chronic cardiovascular diseases including atherosclerosis, thrombosis, reperfusion injury and restenosis after percutaneous transluminal coronary angioplasty etc. Inhibition of cells adhesion of pathological status is effective in reducing the tissue injury induced by acute or chronic inflammations and in preventing the genesis and progress of the diseases mentioned above. It is known that several factors influence the cells adhesion. To study these regulating factors, especially drugs, may be of considerable importance for basic theory and clinical application.
The adhesion of leukocyte/platelet was very low in healthy volunteers and normal animals. It was increased dramatically in patients with acute myocardial ischaemia (AMI) or experimental pathological animal models. The results indicated that the activation of both leukocyte and platelet played an important role in increasing both cells adhesion in AMI. The leukocyte/platelet adhesion rate was also increased when platelets were stimulated by thrombin in vitro. The adhesion of leukocyte/platelet was Ca~(2+) dependent, heparin or dextran sulfate inhibited both cells binding but not dextran. This indicates that adhesion molecule—GMP-140 might be involved in the mechanism of leukocyte/platelet adhesion. In addition, the expression of CDlla and CD 11b on the surface of leukocyte in AMI patients was significantly increased than normal subjects. It suggests that the adhesion molecules— CD11a/CD18 and CD11b/CD18 may mediate leukocyte/platelet adhesioa
Quercetin inhibited the leukocyte/platelet adhesion in AMI animals when administered intravenously before the formation of AMI model or before the platelet stimulated by thrombin. Aspirin had similar effects as quercetin.
The results showed that unactivated leukocyte inhibited platelets aggregation. Both quercetin and aspirin enhanced the inhibitory effects of leukocyte on platelet activation. However, the inhibition of platelet aggregation was disappeared when leukocyte was activated by PMA. It is interested to find out that if incubation of quercetin with platelets before activation of leukocyte by PMA, the inhibitory effect of leukocyte on platelet aggregation was restored. It indicates that quercetin has the ability to prevent the activation of leukocyte by PMA.
TNF_α increased the adhesion of EC to leukocyte both time- and dose-dependently. More monocytes bound to EC than neutrophils, and it became more significant with the prolongation of stimulation time. Anti-ICAM-1, anti-E-selectin and anti-VCAM-1 monoclonal antibodies blocked the binding of TNF_α stimulated EC to leukocyte, suggesting that the adhesion molecules, ICAM-1, E-selectin and VCAM-1, mediated both cells adhesion.
ELISA and flow cytometric analysis revealed that the expression of ICAM-1 and
引文
1. Marcus AJ, Safier LB. Thromboregulation: Multicellular modulation of platelet reactivity in hemostasis and thrombosis. FASEB J. 1993, 7(6); 516-522
2．李福刚，汪钟 白细胞与内皮细胞、血小板粘附的分子机理及意义。中国病理生理杂志；10：333-336，1994
3. Salvemini D, Necci G, Gryglewski RJ, Vane JR. Human neutrophils and mononuclear cells inhibit platelet aggregation by releasing a nitric oxide-like factor. Proc Natl Acad Sci. 1989, 86(16); 6328-6332
4. Maschio AD, Evangelista V, Bajtar G, Chen ZM, Cerletti C, Gaetano G. Platelet activation by polymorphonuclear leukocytes exposed to chemotactic agent. Am J Physiol. 1990, 258(3); H870-H879
5. Lopez-Farre A, Caramelo C, Esteban A, alberola ML, Millas, I, Monton M, et al. Effects of aspirin on platelet-neutrophil interactions. Circulation 1995, 91(7); 2080-2088
6. Li FG, Wang ZY, Ruan CG, Gu QL, Zhang J. The effects of quercetin on leukocyte function and metabolism. Chinese Pharmacology Bulletin 1991, 7(6); 466-4697. Song ZJ, Liu W, Liang NC. Quercetin inhibits actin polymerization of pig platelets induced by thrombin. Chinese Biochemical Journal 1997, 13(4); 490-492
8. Chignier E, Sparagano MH, McGregor L, Thillier A, Pellecchia D, McGregor JL. Two sites (23-30, 76-90) on rat P-selectin mediate thrombin activated platelet-neutrophil interactions. Comp Biochem Physiol. 1994, 109A(4); 881-886
9. Skinner MP, et al. GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem 1991,266(9):5371-5374
10. Hamburger SA, McEver RP. GMP-140 mediates adhesion of stimulated platelets to neutrophils . Blood 1990,75:550-554
11．陆继福，等，血小板颗粒膜蛋白在急性心梗治疗中的动态变化．血栓与止血杂志 1994，1：25-27
12. Ono M, et al. Detection of CD62P(GMP-140) on peripheral blood platelet membrane in various heart diseases. Jpn J Clin Pathol,1995,43:438-441
13. Lalor P, Nash GB. Adhesion of flowing leukocytes to immobilized platelets. Br J Haematol 1995,89:725-732
14. Li F, et al. Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies. J Biol Chem 1996,271:6342-6349
15. Li F, et al. Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin. J Biol Chem 1996, 271(6):3255-3264
16. Lopez-Farre A, riesco A, Digiuni E, Mosquera JR., Caramelo C, Miguel LS, et al. Aspirin-stimulated nitric oxide production by neutrophils after acute myocardial ischemia in rabbits. Circulation 1996, 94(1); 83-87
17. Colli S, Eligini S, Lalli M, Tremoli E. Platelet-neutrophil interaction and superoxide anion generation: Involvement of purine nucleotides. Free Radical Biology & Medicine 1996, 20(3); 271-27818. Rand ML, Pern' DW, Packham MA, Gemmell CH, Yeo EL, Kinlough- Rathbone RL. Conditions influencing release of granule contents from human platelets in citrated plasma induced by ADP or the thrombin receptor activating petide SFLLRN: Direct measurement of percent release of β-thromboglobulin and assessment by flow cytometry of P-selectin expression. Am. J. Hematol. 1996, 52(2); 288-294
1. Rothlein R, Czajkowski M, Kishimoto TK, Intercellular Adhesion Molecule-1 in the Inflammatory Response. Chem Immunol.50, 135-142, 1991
2. Wayne Smith C, Leukocyte-Endothelial Cell Interactions. Seminars in Hematology. 30(4), Suppl 4,45-55 1993:
3. Albelda ST, Wayne Smith C, Ward PA, Adhesion Molecules and Inflammatory Injury. FASEBJ. 8: 504-512; 1994
4. Wallis WJ, Beatty PG, Ochs HD, Human Monocyte Adherence to Cultured Vascular Endothelium: Monoclonal Antibody-Defined Mechanisms. J Immunology 135(4), 2323-2330, 1985,
5. Pober JS, Bevilacqua MP, Menerick DL, Two Distinct Monokines, Interleukin 1 and Tumor Necrosis Factor, Each Independently Induce Biosynthesis and Transient Expression of the Same Surface of Cultured Human Vascular Endothelial Cells. J Immunol, 136(5), 1680-1687,1986
6. Van de Stolpe A, Van der Saag PT, Intercellular Adhesion Molecule-1. J.Mol.Med 74: 13-33,1996
7. Pietersma A, Koster JF, Sluiter W, Effect of Hypoxia on Adherence of Granulocytes to Endothelial Cells in vitro. Heart Circ Physiol 36: H874-H879, 1994
8. Gimbrone MA, Cotran RS, Folkman J, Human Vascular Endothelial Cells in Culture Growth and DNA Synthesis. J Cell Biol. 60, 673-684,1974
9. Maciag T, Hoover GA, Stemerman MB, Serial Propagation of Human Endothelial Cells In Vitro. J Cell Biology. 91, 420-426 ,1981
10. Lockyer JM,Colladay JS,Alperin-Lea WL, Inhibition of Nuclear Factor-kB-Mediated Adhesion Molecule Expression in Human Endothelial Cells. Circ Res 82: 314-320, 199811. Grimm S. Baeuerle PA, The inducible transcription factor NF-kB: structure-function relationship of its protein subunits. Biochem J 290, 297-308,1993
12. Bradley PP, Priebat DA, Christensen RD, Measurement of Cutaneous Inflammation : Estimation of Neutrophil Content with an Enzyme Marker. The Journal of Investigative Dermatology, 78: 206-209, 1982
13. Yeao PS, Later AM, Antibody to CD-18 Exerts Endothelial and Cardiac Protective Effects in Myocardial ischemia and Reperfusioa J.Clin. Invest. 88,1237-1243, 1991
14. Arnould BT, Michiels C, Remade J, Hypoxic Human Umbilical Vein Endothelial Cells Induce Activation of Adherent Polymorphonuclear Leukocytes. Blood, 83, 3705-3716, 1994
15. Klein CL, Bittinger F, Kirkpatrick CJ, Comparative Studies on Vascular Endothelium in Vitro. Pathobiology 63: 1-8,1995;
16. Jang Y, Lincoff AM, Plow EF, Cell Adhesion Molecules in Coronary Artery Disease. J Am Coll Cardiol. 24,1591-1601,1994
17. Miles DW, Happerfield LC, Bobrow LG, Adhesion Molecule Expresion and Leucocyte Trafficking Following Immunotherapy With Recombinant Interleukin-2. Histopathology, 28, 301-308,1996
18. Foster CA, VCAM-1/α4-integrin adhesion pathway: Therapeutic target for allegic inflammatory disorders. J Allergy Clin Immunol; 98: S270-S277, 1996
19. Gille J, Paxtron LLL, Lawley TJ, Retinoic Acid Inhibits the Regulated Expression of Vascular Cell Adhesion Molecule-1 by Cultured Dermal Microvascular Endothelial Cells. J. Clin Invest,99(3), 492-500,1997
20. Huang ZH, Bates EJ, Ferrante JV, Inhibition of Stimulus-Induced Endothelial Cell Intercellular Adhesion Molecule-1, E-selectin ,and Vascular Cellular Adhesion Molecule-1 Expression by Arachidonic Acid and Its Hydroxy And Hydroperoxy Derivatived. Circ Res, 80: 149-158,1997
21. O'Brien KD, McDonald TO, Chait A, Neovascular Expression of E-selectin, Intercellular Adhesion Molecule-1, and Vascular Cell Adhesion Molecule-1 in Human Atherosclerosis and Their Relation to Intimal Leukocyte Content. Circulation. 93: 672-682, 1996
22. Shrikant P, Joong Lee S, Kalvakolanu I, Stimulus-Specific Inhibition of Intercellular Adhesion Molecule-1 Gene Expression by TGF-β1. J Immunology, 157: 892-900,1996
23. Kacimi R, Karliner JS, Koudssi F, Expression and Regulation of Adhesion Molecules in Cardiac Cells by Cytokines. Circ Res. 82: 576-586,1998
24. Nelson RM, Dolich S,Aruffo A, Higher-Affinity Oligosaccharide Ligands for E-selectin. J.Clialnvest. 91, 1157-1166,199325. Pazton LLL, Li LJ, Secor V, Flanking Sequences for the Human Intercellular Adhesion Molecule-1 NF-kB Response Element Are Necessary for Tumor Necrosis Factor α-Induced Gene Expressioa. J Biological Chemistry. 272(25), 15928-15935, 1997
26. Anrather J, Csizmadia V, Brostjan C, Inhibition of Bovine Endothelial Cell Activation In Vitro by Regulated Expression of a Transdominant Inhibitor of NF-kB. J.Clin.Invest, 99(4), 763-772,1997
27. Weber C, Erl W, Pietsch A, Aspirin Inhibits Nuclear Factor-kB Mobilization and Monocyte Adhesion in Stimulated Human Endothelial Cells. Circulation. 91: 1914-1917,19951. Gown AM, Tsukada T, Ross R, Human atherosclerosis: Ⅱ Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions Am J Pathol 125,191-207, 1986
2. Holvoet P, Collen D, Thrombosis and atherosclerosis. Curr Opin Lipidol, 8(5): 320-328, 1997
3. Nilsson J. Cytokines and smooth muscle cells in atherosclerosis, Cardiovasc Res.27,1184-90,1993
4. Totsuka T, Tanimoto H, Yamaguchi T, Invasive human T lymphoma cells produce a novel factor that upregulates expression of adhesion molecules on endothelial cells. Experimental Hematology 21: 1544-1549,1993
5. Duplaa C, Couffinhal T, Dufourcq P, The Integrin Very Late Antigen-4 Is Expressed in Human Smooth Muscle Cell ,Involvement of α4 and Vascular Cell Adhesion Molecule-1 During Smooth Muscle Cell Differentiation. Circ Res 80: 159-169, 1997
6. Clinton SK, Libby P Cytokines and growth factors in atherogenesis. Arch Pathol Lab Med,116,1292-1300, 1992
7. Wang XK, Feuerstein GZ, Gu JL Interleukin-1β induces expression of adhesion molecules in human vascular smooth muscle cells and enhances adhesion of leukocytes to smooth muscle cells. Atherosclerosis 115:89-98,1995
8. Platts KE, Benson MT, Rennie IG, Cytokine Modulation of Adhesion Molecule Expression on Human Retinal Pigment Epithelial Cells. Invest Ophthalmol Vis Sci. 36: 2262-2269,1995
9. Wang XK, Feuerstein GZ, Clark RK, Enhanced leucocyte adhesion to interleukin-1β stimulated vascular smooth muscle cells is mainly through intercellular adhesion molecule-1. Cardiovascular Research 28: 1808-1814, 1994
10. Stavri GT, Zachary IC, Baskerville PA, Basic Fibroblast Growth Factor Upregulates the Expression of Vascular Endothelial Growth Factor in Vascular Smooth Muscle Cells, Synergistic interaction with hyopxia. Circulation. 92: 11 -14,1995
11. Poston RN, Haskard DO, Coucher JR, et al. Expression of intercellular adhesion molecule-1 in atherosclerotic plaques. Am J Pathol, 140. 665-673,1992
12. Printaeva OY, Peclo MM, Gown AM Various cell type in human atherosclerotic lesions xpress ICAM-1: futher immunocytochemical and immunochemical studies employing monclonal antibody 10F3, Am J Pathol 140,889-896, 1992
13. Couffinhal T, Duplaa C, Labat L, et al Tumor necrosis factor-α stimulates ICAM-1 expression in human vascular smooth mucscle cells, Arteriosclerosis Thromb 13; 407-414,199314. Li H， Cybulsky MI, Gimbrone MA, et al Inducible expression of vascular cell adhesion molecule-1 by vascular smooth muscle cells in vitro and within rabbit atheroma. Am J Pathol, 143;1551-1559, 1993
15. Couffinhal T. Duplaa C, Moreau C, Regulation of Vascular Cell Adhesion Molecule-1 and Intercellular Adhesion Molecule-1 in Human Vascular Smooth Muscle Cells. Circ Res 74: 225-234.1994
16. Davies MJ, Gordon JL, Gearing AJH, et al. The expression of the adhesion molecules ICAM-1,VCAM-1, PECAM, and E-selectin in human atherosclerosis J Pathol 171: 223-9,1993
17. Kita Y, Takashi T. Iigo Y, et al, Sequence and expression of rat ICAM-1, Biochim Biophys Acta, 1131, 108-110,1992
18. Wan XK, Siren AL, Yue TL et al. Upregulation of intercellular adhesion molecule-1 (ICAM-1) on brain microvascular endothelial cells in rat ischemic cortex. Mol Brain Res 26, 61-68, 1994
19. Hession C, Moy P, Tizard R, et al, Cloning of murine and rat vascular cell adhesion molecule 1, Biochem Biophys Res Commun, 183,163-169,1992
20. Van de Stolpe A, Van der Saag PT, Intercellular Adhesion Molecule-1. J.Mol.Med 74: 13-33,1996
21. Kacimi R, Karliner JS, Koudssi F, Expression and Regulation of Adhesion Molecules in Cardiac Cells by Cytokines. Circ Res. 82: 576-586,1998
22. Miles DW, Happerfield LC, Bobrow LG, Adhesion Molecule Expresion and Leucocyte Trafficking Following Immunotherapy With Recombinant Interleukin-2. Histopathology, 28, 301-308,1996
23. Anrather J, Csizmadia V, Brostjan C, Inhibition of Bovine Endothelial Cell Activation In Vitro by Regulated Expression of a Transdominant Inhibitor of NF-kB. J.Clin.Invest, 99(4), 763-772,1997
24. Weber C, Erl W, Pietsch A, Aspirin Inhibits Nuclear Factor-kB Mobilization and Monocyte Adhesion in Stimulated Human Endothelial Cells. Circulatioa 91: 1914-1917,1995
25. Grimm S, Baeuerle PA, The inducible transcription factor NF-kB: structure-function relationship of its protein subunits. Biochem J 290, 297-308,19931, Alderson LM, Endemann G, Lindsey S. LDL enhances monocyte adhesion to endothelial cells in vitro. Am J Pathol. 123: 334-341, 1986
2, Kume N, Cybulsky MI, Gimbrone MJ. Lysophosphatidylcholine, a component of atherogenic lipoprotein, induces mononuclear leukocyte adhesion molecules in cultured human and rabbit arterial endothelial cells. J Clin Invest. 90: 1138-1144, 1992
3, Jeng JR, Chang CH, Shieh SM. Oxidized low-density lipoprotein enhances monocyte-endothelial cell binging against shear-stress-induced detachment. Biochim Biophys Acta 1178:221-227, 1993
4, Frostegard J, Wu R, Haegerstrand A. Mononuclear leukocytes exposed to oxidized low density lipoprotein secrete a factor that stimulates endothelial cells to express adhesion molecules. Atherosclerosis. 103:213-219, 1993
5, Kim JA, Territo MC, Wayner E. Partial Characterization of leukocyte Binding Molecules on Endothelial Cells Induced by Minimally Oxidized LDL. Arterioscler Thromb. 14: 427-433, 1994
6, Haller H, Schaper D, Ziegler W. Low-Density Lipoprotein Induces Vascular Adhesion Molecule Expression on Human Endothelial Cells, Hypertension.25: 511-516, 1995
7, Khan BV, Parthasarathy SS, Alexander RW. Modified low density??lipoprotein augment cytokine-activated vascular cell adhesion molecule-1 gene expression in human vascular endothelial cells. J Clin Invest. 95:1262-1270, 1995
8, Smalley DM, Lin JHC, Curtis ML. Native LDL Increases Endothelial Cell Adhesiveness by Inducing Intercellular Adhesion Molecule-1. Arterioscler Thromb Vasc Biol. 16: 585-590, 1996
9、翁进，朱雪静，林勇等．甘糖酯对血小板与内皮细胞功能的影响．中国医学科学院学报，19：414-418，1997
10、唐蔚青，王抒，黎健 野生型P53基因导入诱导平滑肌细胞凋亡。中国动脉硬化杂志，5：194-198，1997
11, Bennett MR, Evan GI, Schwartz SM. Apoptosis of Rat Vascular Smooth Muscle Cells Is Regulated by p53-Denendent and-Independent Pathways. Circ Res. 77: 266-273, 1995
12, 12Berliner JA, Territo MC, Sevanian A. Ramin S. Minimally modified LDL stimulates monocyte endothelial interactions. J Clin Invest. 85: 1260-1266, 1990
13, 13Frostegard J, Haegerstrand A, Gidlund M. Biologically modified LDL increases the adhesive properties of endothelial cells. Atherosclerosis. 90: 119-126, 1991
14, Cominacini L, Garbin U, Pasini AF. Antioxidants Inhibit the Expression of Intercellular Cell Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Induced by Oxidized LDL on Human Umbilical Vein Endothelial Cells. Free Radical Biology & Medicine. 22:117-127, 1997
15, Faggiotto A, Ross R, Harker L. Studies of hypercholesterolemia in the non-human primate, I: changes that lead to fatty streak formation. Arteriosclerosis. 4: 323-340, 1986
16, Witztum JL, Steinberg D. Role of oxidized low density liprotein??in atherogenesis. J Clin Invest. S8: 1785-1792. 1991
17, Steinberg D, Parthasarathy S, Carew TE. Beyond cholesterol: modifications of low-density lipoprotein that increase its atherogeneity. N Engl J Med. 320: 915-919, 1989
18, Maier JAM, Atatuto M, Pagnotti G. Senescence Stimulates U937- Endothelial Cell Interactions. Experimental Cell Research. 208:270-274, 1993
19, Minter AJ, Keoshkerian E, Chesterman CN. Fibroblast Growth Factor and Heparin Protect Endothelial Cells From the Effects of Interleukin 1. Journal of Cellular Physiology. 167: 229-237, 1996
20, Watanabe Y, Dvorak HF. Vascular permeability factor/vacular endothelial growth factor inhibits,anchorage-disruption-induced apoptosis in microvessel endothelial cells by inducing scaffold formation. Exp Cell Res, 233: 340-349, 1997
21, Ginis I, Faller DV. Protection from apoptosis in human neutrophils is determined by the surface of adhesion. Am J Physiol, 272: C295-C309, 1997
22, Chen CS, MrksichM, Huang S. Geometric control of cell life and death. Science, 276(5317): 1425-1428, 1997
23, Hoyt DG, Mannix RJ, Watkins SC. Integrins inhibit LPS-induced DNA strand breakage in cultured lung endothelial cells. Am J Physiol, 270: L689-94, 1996
24, Miao JY, Araki S, Kaji K. Integrin beta4 is involved in apoptotic signal transduction in endothelial cells. Biochem Biophys Res Commun, 233: 182-186, 1997
25, Lockyer JM, Colladay JS, Alperin-Lea WL. Inhibition of nuclear factor-KB-mediated adhesion molecule expression in human endothelial cells. Circ Res. 82: 314-320, 1998
1. Muller U, Wang D, Denda S, et al. Integrin α8β1 is critically important for epithelial-messenchymal interactions during kidney morphogenesis. Cell 1997:88:603-613
2. Duplaa C, Couffinhal T, Dufourcq P, et al. The integrin very late antigen-4 is expressed in human smooth muscle cell—Involvement of α4 and vascular cell adhesion molecule-1 during smooth muscle cell differentiation. Circ Res. 1997:80:159-169
3. Collins T, Williams A, Johnston GI, et al. Structure and chromosomal location of the gene for endothelial-leukocyte adhesion molecule 1. J Biol Chem 1991;266:2468-2473
4. Li F, Wilkins PP, Crawley S, et al. Post-translatonal modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P-and E-selectin. J Biol Chem. 1996;271;3255-3264
5. Chen XL, Tummala PE, Olliff L, et al. E-selectin gene expression in vascular smooth muscle cells. Circ Res. 1997:80:305-311
6. Benjamin C, Dougas I, Chi-Rosso G, et al. A blocking monoclonal antibody to endothelial-leukocyte adhesion molecule-1(ELAM1). Biochem Biophy Res Commun 1990, 171, 348-3537. Bajt ML. Goodman T and McGuire SL. β2(CD18) mutations abolish ligand recognition by I domain integrins LFA-l(αLβ2, CD11a/CD18) and Mac-1 (αLβ2, CDllb, CD18). J Biol Chem. 1995, 270, 94-98.
8. Skinner MP, Lucas CM, Burns GF, et al. GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem. 1991,266, 5371-5374
9. Wilson RW, Ballantyne CM, Smith CW, et al. Gene targeting yields a CD18-mutant mouse for study of inflammation. J Immunol 1993; 151; 1571-1578.
10. Zimmerman GA, McIntyre TM, Mehra Meena et al. Endothelial cell -associated platelet-activation factor: a novel mechanism for signaling intercellular adhesion. J Cell Biology 1990,110,529-540
11.Spertini O, Kansas GS, Munro JM, et al. Regulation of leukocyte migration by activation of the leukocyte adhesion molecule-1(LAM-1) selection. Nature. 1991, 349, 691-694.
12. Finger EB, Puri KD, Alon R, et al. Adhesion through L-selectin requires a threshold hydrodynamic shear. Nature, 1996, 379, 266-269
13. Lehr HA, Nolte D, Hubner C, et al. Impact of lipid mediator on leukocyte/endothelium interaction. Prog Appl Microcirc 1991;18;82-90.
14. Arndt H, Palitzsch K-D, Anderson DC, et al. Leucocyte-endothelial cell adhesion in a model of intestinal inflammation. Gut 1995;37;374-379.
15. Kunkel EJ and Ley K. Distinct phenotype of E-selectin-deficient mice, E-selectin is required for slow leukocyte rolling in vivo. Circ Res. 1996; 79:1196-1204
16.Weller A, Isenmann S and Vestweber D. Cloning of the mouse endothelial selectins, expression of both E- and P-selectin is inducible by tumor necrosis factor. J Biol Chem 1992, 267, 15176-15183
17. Maier JAM, Statuto M and Ragnotti G, Senescence stimulates U937-Endothelial cell interactions. Exp. Cell Res 1993;208, 270-274.
18. Weber C, Erl W, Pietsch A, et al, Aspirin inhibits nuclear factor-kB mobilization and monocyte adhesion in stimulated human endothelial cells. Circulation 1995;91;1914-1917,.
19. Huang ZH, Bates EJ, Ferrante JV et al. Inhibition of stimulus-induced endothelial cell intercellular adhesion molecule-1, E-selectin, and vascular cellular adhesion molecule-1 expression by arachidonic acid and its hydroxy and hydroperoxy derivatives. Circ Res. 1997; 80: 149-158
20. Minter AJ, Keoshkerian E, Chesterman CN et al. Fibroblast growth factor and heparin protect endothelial cells from the effects of interleukin 1. J Cell Physiol. 1996:167:229-237
2．李福刚，汪钟 白细胞与内皮细胞、血小板粘附的分子机理及意义。中国病理生理杂志；10：333-336，1994
3. Salvemini D, Necci G, Gryglewski RJ, Vane JR. Human neutrophils and mononuclear cells inhibit platelet aggregation by releasing a nitric oxide-like factor. Proc Natl Acad Sci. 1989, 86(16); 6328-6332
4. Maschio AD, Evangelista V, Bajtar G, Chen ZM, Cerletti C, Gaetano G. Platelet activation by polymorphonuclear leukocytes exposed to chemotactic agent. Am J Physiol. 1990, 258(3); H870-H879
5. Lopez-Farre A, Caramelo C, Esteban A, alberola ML, Millas, I, Monton M, et al. Effects of aspirin on platelet-neutrophil interactions. Circulation 1995, 91(7); 2080-2088
6. Li FG, Wang ZY, Ruan CG, Gu QL, Zhang J. The effects of quercetin on leukocyte function and metabolism. Chinese Pharmacology Bulletin 1991, 7(6); 466-4697. Song ZJ, Liu W, Liang NC. Quercetin inhibits actin polymerization of pig platelets induced by thrombin. Chinese Biochemical Journal 1997, 13(4); 490-492
8. Chignier E, Sparagano MH, McGregor L, Thillier A, Pellecchia D, McGregor JL. Two sites (23-30, 76-90) on rat P-selectin mediate thrombin activated platelet-neutrophil interactions. Comp Biochem Physiol. 1994, 109A(4); 881-886
9. Skinner MP, et al. GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem 1991,266(9):5371-5374
10. Hamburger SA, McEver RP. GMP-140 mediates adhesion of stimulated platelets to neutrophils . Blood 1990,75:550-554
11．陆继福，等，血小板颗粒膜蛋白在急性心梗治疗中的动态变化．血栓与止血杂志 1994，1：25-27
12. Ono M, et al. Detection of CD62P(GMP-140) on peripheral blood platelet membrane in various heart diseases. Jpn J Clin Pathol,1995,43:438-441
13. Lalor P, Nash GB. Adhesion of flowing leukocytes to immobilized platelets. Br J Haematol 1995,89:725-732
14. Li F, et al. Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies. J Biol Chem 1996,271:6342-6349
15. Li F, et al. Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin. J Biol Chem 1996, 271(6):3255-3264
16. Lopez-Farre A, riesco A, Digiuni E, Mosquera JR., Caramelo C, Miguel LS, et al. Aspirin-stimulated nitric oxide production by neutrophils after acute myocardial ischemia in rabbits. Circulation 1996, 94(1); 83-87
17. Colli S, Eligini S, Lalli M, Tremoli E. Platelet-neutrophil interaction and superoxide anion generation: Involvement of purine nucleotides. Free Radical Biology & Medicine 1996, 20(3); 271-27818. Rand ML, Pern' DW, Packham MA, Gemmell CH, Yeo EL, Kinlough- Rathbone RL. Conditions influencing release of granule contents from human platelets in citrated plasma induced by ADP or the thrombin receptor activating petide SFLLRN: Direct measurement of percent release of β-thromboglobulin and assessment by flow cytometry of P-selectin expression. Am. J. Hematol. 1996, 52(2); 288-294
1. Rothlein R, Czajkowski M, Kishimoto TK, Intercellular Adhesion Molecule-1 in the Inflammatory Response. Chem Immunol.50, 135-142, 1991
2. Wayne Smith C, Leukocyte-Endothelial Cell Interactions. Seminars in Hematology. 30(4), Suppl 4,45-55 1993:
3. Albelda ST, Wayne Smith C, Ward PA, Adhesion Molecules and Inflammatory Injury. FASEBJ. 8: 504-512; 1994
4. Wallis WJ, Beatty PG, Ochs HD, Human Monocyte Adherence to Cultured Vascular Endothelium: Monoclonal Antibody-Defined Mechanisms. J Immunology 135(4), 2323-2330, 1985,
5. Pober JS, Bevilacqua MP, Menerick DL, Two Distinct Monokines, Interleukin 1 and Tumor Necrosis Factor, Each Independently Induce Biosynthesis and Transient Expression of the Same Surface of Cultured Human Vascular Endothelial Cells. J Immunol, 136(5), 1680-1687,1986
6. Van de Stolpe A, Van der Saag PT, Intercellular Adhesion Molecule-1. J.Mol.Med 74: 13-33,1996
7. Pietersma A, Koster JF, Sluiter W, Effect of Hypoxia on Adherence of Granulocytes to Endothelial Cells in vitro. Heart Circ Physiol 36: H874-H879, 1994
8. Gimbrone MA, Cotran RS, Folkman J, Human Vascular Endothelial Cells in Culture Growth and DNA Synthesis. J Cell Biol. 60, 673-684,1974
9. Maciag T, Hoover GA, Stemerman MB, Serial Propagation of Human Endothelial Cells In Vitro. J Cell Biology. 91, 420-426 ,1981
10. Lockyer JM,Colladay JS,Alperin-Lea WL, Inhibition of Nuclear Factor-kB-Mediated Adhesion Molecule Expression in Human Endothelial Cells. Circ Res 82: 314-320, 199811. Grimm S. Baeuerle PA, The inducible transcription factor NF-kB: structure-function relationship of its protein subunits. Biochem J 290, 297-308,1993
12. Bradley PP, Priebat DA, Christensen RD, Measurement of Cutaneous Inflammation : Estimation of Neutrophil Content with an Enzyme Marker. The Journal of Investigative Dermatology, 78: 206-209, 1982
13. Yeao PS, Later AM, Antibody to CD-18 Exerts Endothelial and Cardiac Protective Effects in Myocardial ischemia and Reperfusioa J.Clin. Invest. 88,1237-1243, 1991
14. Arnould BT, Michiels C, Remade J, Hypoxic Human Umbilical Vein Endothelial Cells Induce Activation of Adherent Polymorphonuclear Leukocytes. Blood, 83, 3705-3716, 1994
15. Klein CL, Bittinger F, Kirkpatrick CJ, Comparative Studies on Vascular Endothelium in Vitro. Pathobiology 63: 1-8,1995;
16. Jang Y, Lincoff AM, Plow EF, Cell Adhesion Molecules in Coronary Artery Disease. J Am Coll Cardiol. 24,1591-1601,1994
17. Miles DW, Happerfield LC, Bobrow LG, Adhesion Molecule Expresion and Leucocyte Trafficking Following Immunotherapy With Recombinant Interleukin-2. Histopathology, 28, 301-308,1996
18. Foster CA, VCAM-1/α4-integrin adhesion pathway: Therapeutic target for allegic inflammatory disorders. J Allergy Clin Immunol; 98: S270-S277, 1996
19. Gille J, Paxtron LLL, Lawley TJ, Retinoic Acid Inhibits the Regulated Expression of Vascular Cell Adhesion Molecule-1 by Cultured Dermal Microvascular Endothelial Cells. J. Clin Invest,99(3), 492-500,1997
20. Huang ZH, Bates EJ, Ferrante JV, Inhibition of Stimulus-Induced Endothelial Cell Intercellular Adhesion Molecule-1, E-selectin ,and Vascular Cellular Adhesion Molecule-1 Expression by Arachidonic Acid and Its Hydroxy And Hydroperoxy Derivatived. Circ Res, 80: 149-158,1997
21. O'Brien KD, McDonald TO, Chait A, Neovascular Expression of E-selectin, Intercellular Adhesion Molecule-1, and Vascular Cell Adhesion Molecule-1 in Human Atherosclerosis and Their Relation to Intimal Leukocyte Content. Circulation. 93: 672-682, 1996
22. Shrikant P, Joong Lee S, Kalvakolanu I, Stimulus-Specific Inhibition of Intercellular Adhesion Molecule-1 Gene Expression by TGF-β1. J Immunology, 157: 892-900,1996
23. Kacimi R, Karliner JS, Koudssi F, Expression and Regulation of Adhesion Molecules in Cardiac Cells by Cytokines. Circ Res. 82: 576-586,1998
24. Nelson RM, Dolich S,Aruffo A, Higher-Affinity Oligosaccharide Ligands for E-selectin. J.Clialnvest. 91, 1157-1166,199325. Pazton LLL, Li LJ, Secor V, Flanking Sequences for the Human Intercellular Adhesion Molecule-1 NF-kB Response Element Are Necessary for Tumor Necrosis Factor α-Induced Gene Expressioa. J Biological Chemistry. 272(25), 15928-15935, 1997
26. Anrather J, Csizmadia V, Brostjan C, Inhibition of Bovine Endothelial Cell Activation In Vitro by Regulated Expression of a Transdominant Inhibitor of NF-kB. J.Clin.Invest, 99(4), 763-772,1997
27. Weber C, Erl W, Pietsch A, Aspirin Inhibits Nuclear Factor-kB Mobilization and Monocyte Adhesion in Stimulated Human Endothelial Cells. Circulation. 91: 1914-1917,19951. Gown AM, Tsukada T, Ross R, Human atherosclerosis: Ⅱ Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions Am J Pathol 125,191-207, 1986
2. Holvoet P, Collen D, Thrombosis and atherosclerosis. Curr Opin Lipidol, 8(5): 320-328, 1997
3. Nilsson J. Cytokines and smooth muscle cells in atherosclerosis, Cardiovasc Res.27,1184-90,1993
4. Totsuka T, Tanimoto H, Yamaguchi T, Invasive human T lymphoma cells produce a novel factor that upregulates expression of adhesion molecules on endothelial cells. Experimental Hematology 21: 1544-1549,1993
5. Duplaa C, Couffinhal T, Dufourcq P, The Integrin Very Late Antigen-4 Is Expressed in Human Smooth Muscle Cell ,Involvement of α4 and Vascular Cell Adhesion Molecule-1 During Smooth Muscle Cell Differentiation. Circ Res 80: 159-169, 1997
6. Clinton SK, Libby P Cytokines and growth factors in atherogenesis. Arch Pathol Lab Med,116,1292-1300, 1992
7. Wang XK, Feuerstein GZ, Gu JL Interleukin-1β induces expression of adhesion molecules in human vascular smooth muscle cells and enhances adhesion of leukocytes to smooth muscle cells. Atherosclerosis 115:89-98,1995
8. Platts KE, Benson MT, Rennie IG, Cytokine Modulation of Adhesion Molecule Expression on Human Retinal Pigment Epithelial Cells. Invest Ophthalmol Vis Sci. 36: 2262-2269,1995
9. Wang XK, Feuerstein GZ, Clark RK, Enhanced leucocyte adhesion to interleukin-1β stimulated vascular smooth muscle cells is mainly through intercellular adhesion molecule-1. Cardiovascular Research 28: 1808-1814, 1994
10. Stavri GT, Zachary IC, Baskerville PA, Basic Fibroblast Growth Factor Upregulates the Expression of Vascular Endothelial Growth Factor in Vascular Smooth Muscle Cells, Synergistic interaction with hyopxia. Circulation. 92: 11 -14,1995
11. Poston RN, Haskard DO, Coucher JR, et al. Expression of intercellular adhesion molecule-1 in atherosclerotic plaques. Am J Pathol, 140. 665-673,1992
12. Printaeva OY, Peclo MM, Gown AM Various cell type in human atherosclerotic lesions xpress ICAM-1: futher immunocytochemical and immunochemical studies employing monclonal antibody 10F3, Am J Pathol 140,889-896, 1992
13. Couffinhal T, Duplaa C, Labat L, et al Tumor necrosis factor-α stimulates ICAM-1 expression in human vascular smooth mucscle cells, Arteriosclerosis Thromb 13; 407-414,199314. Li H， Cybulsky MI, Gimbrone MA, et al Inducible expression of vascular cell adhesion molecule-1 by vascular smooth muscle cells in vitro and within rabbit atheroma. Am J Pathol, 143;1551-1559, 1993
15. Couffinhal T. Duplaa C, Moreau C, Regulation of Vascular Cell Adhesion Molecule-1 and Intercellular Adhesion Molecule-1 in Human Vascular Smooth Muscle Cells. Circ Res 74: 225-234.1994
16. Davies MJ, Gordon JL, Gearing AJH, et al. The expression of the adhesion molecules ICAM-1,VCAM-1, PECAM, and E-selectin in human atherosclerosis J Pathol 171: 223-9,1993
17. Kita Y, Takashi T. Iigo Y, et al, Sequence and expression of rat ICAM-1, Biochim Biophys Acta, 1131, 108-110,1992
18. Wan XK, Siren AL, Yue TL et al. Upregulation of intercellular adhesion molecule-1 (ICAM-1) on brain microvascular endothelial cells in rat ischemic cortex. Mol Brain Res 26, 61-68, 1994
19. Hession C, Moy P, Tizard R, et al, Cloning of murine and rat vascular cell adhesion molecule 1, Biochem Biophys Res Commun, 183,163-169,1992
20. Van de Stolpe A, Van der Saag PT, Intercellular Adhesion Molecule-1. J.Mol.Med 74: 13-33,1996
21. Kacimi R, Karliner JS, Koudssi F, Expression and Regulation of Adhesion Molecules in Cardiac Cells by Cytokines. Circ Res. 82: 576-586,1998
22. Miles DW, Happerfield LC, Bobrow LG, Adhesion Molecule Expresion and Leucocyte Trafficking Following Immunotherapy With Recombinant Interleukin-2. Histopathology, 28, 301-308,1996
23. Anrather J, Csizmadia V, Brostjan C, Inhibition of Bovine Endothelial Cell Activation In Vitro by Regulated Expression of a Transdominant Inhibitor of NF-kB. J.Clin.Invest, 99(4), 763-772,1997
24. Weber C, Erl W, Pietsch A, Aspirin Inhibits Nuclear Factor-kB Mobilization and Monocyte Adhesion in Stimulated Human Endothelial Cells. Circulatioa 91: 1914-1917,1995
25. Grimm S, Baeuerle PA, The inducible transcription factor NF-kB: structure-function relationship of its protein subunits. Biochem J 290, 297-308,19931, Alderson LM, Endemann G, Lindsey S. LDL enhances monocyte adhesion to endothelial cells in vitro. Am J Pathol. 123: 334-341, 1986
2, Kume N, Cybulsky MI, Gimbrone MJ. Lysophosphatidylcholine, a component of atherogenic lipoprotein, induces mononuclear leukocyte adhesion molecules in cultured human and rabbit arterial endothelial cells. J Clin Invest. 90: 1138-1144, 1992
3, Jeng JR, Chang CH, Shieh SM. Oxidized low-density lipoprotein enhances monocyte-endothelial cell binging against shear-stress-induced detachment. Biochim Biophys Acta 1178:221-227, 1993
4, Frostegard J, Wu R, Haegerstrand A. Mononuclear leukocytes exposed to oxidized low density lipoprotein secrete a factor that stimulates endothelial cells to express adhesion molecules. Atherosclerosis. 103:213-219, 1993
5, Kim JA, Territo MC, Wayner E. Partial Characterization of leukocyte Binding Molecules on Endothelial Cells Induced by Minimally Oxidized LDL. Arterioscler Thromb. 14: 427-433, 1994
6, Haller H, Schaper D, Ziegler W. Low-Density Lipoprotein Induces Vascular Adhesion Molecule Expression on Human Endothelial Cells, Hypertension.25: 511-516, 1995
7, Khan BV, Parthasarathy SS, Alexander RW. Modified low density??lipoprotein augment cytokine-activated vascular cell adhesion molecule-1 gene expression in human vascular endothelial cells. J Clin Invest. 95:1262-1270, 1995
8, Smalley DM, Lin JHC, Curtis ML. Native LDL Increases Endothelial Cell Adhesiveness by Inducing Intercellular Adhesion Molecule-1. Arterioscler Thromb Vasc Biol. 16: 585-590, 1996
9、翁进，朱雪静，林勇等．甘糖酯对血小板与内皮细胞功能的影响．中国医学科学院学报，19：414-418，1997
10、唐蔚青，王抒，黎健 野生型P53基因导入诱导平滑肌细胞凋亡。中国动脉硬化杂志，5：194-198，1997
11, Bennett MR, Evan GI, Schwartz SM. Apoptosis of Rat Vascular Smooth Muscle Cells Is Regulated by p53-Denendent and-Independent Pathways. Circ Res. 77: 266-273, 1995
12, 12Berliner JA, Territo MC, Sevanian A. Ramin S. Minimally modified LDL stimulates monocyte endothelial interactions. J Clin Invest. 85: 1260-1266, 1990
13, 13Frostegard J, Haegerstrand A, Gidlund M. Biologically modified LDL increases the adhesive properties of endothelial cells. Atherosclerosis. 90: 119-126, 1991
14, Cominacini L, Garbin U, Pasini AF. Antioxidants Inhibit the Expression of Intercellular Cell Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Induced by Oxidized LDL on Human Umbilical Vein Endothelial Cells. Free Radical Biology & Medicine. 22:117-127, 1997
15, Faggiotto A, Ross R, Harker L. Studies of hypercholesterolemia in the non-human primate, I: changes that lead to fatty streak formation. Arteriosclerosis. 4: 323-340, 1986
16, Witztum JL, Steinberg D. Role of oxidized low density liprotein??in atherogenesis. J Clin Invest. S8: 1785-1792. 1991
17, Steinberg D, Parthasarathy S, Carew TE. Beyond cholesterol: modifications of low-density lipoprotein that increase its atherogeneity. N Engl J Med. 320: 915-919, 1989
18, Maier JAM, Atatuto M, Pagnotti G. Senescence Stimulates U937- Endothelial Cell Interactions. Experimental Cell Research. 208:270-274, 1993
19, Minter AJ, Keoshkerian E, Chesterman CN. Fibroblast Growth Factor and Heparin Protect Endothelial Cells From the Effects of Interleukin 1. Journal of Cellular Physiology. 167: 229-237, 1996
20, Watanabe Y, Dvorak HF. Vascular permeability factor/vacular endothelial growth factor inhibits,anchorage-disruption-induced apoptosis in microvessel endothelial cells by inducing scaffold formation. Exp Cell Res, 233: 340-349, 1997
21, Ginis I, Faller DV. Protection from apoptosis in human neutrophils is determined by the surface of adhesion. Am J Physiol, 272: C295-C309, 1997
22, Chen CS, MrksichM, Huang S. Geometric control of cell life and death. Science, 276(5317): 1425-1428, 1997
23, Hoyt DG, Mannix RJ, Watkins SC. Integrins inhibit LPS-induced DNA strand breakage in cultured lung endothelial cells. Am J Physiol, 270: L689-94, 1996
24, Miao JY, Araki S, Kaji K. Integrin beta4 is involved in apoptotic signal transduction in endothelial cells. Biochem Biophys Res Commun, 233: 182-186, 1997
25, Lockyer JM, Colladay JS, Alperin-Lea WL. Inhibition of nuclear factor-KB-mediated adhesion molecule expression in human endothelial cells. Circ Res. 82: 314-320, 1998
1. Muller U, Wang D, Denda S, et al. Integrin α8β1 is critically important for epithelial-messenchymal interactions during kidney morphogenesis. Cell 1997:88:603-613
2. Duplaa C, Couffinhal T, Dufourcq P, et al. The integrin very late antigen-4 is expressed in human smooth muscle cell—Involvement of α4 and vascular cell adhesion molecule-1 during smooth muscle cell differentiation. Circ Res. 1997:80:159-169
3. Collins T, Williams A, Johnston GI, et al. Structure and chromosomal location of the gene for endothelial-leukocyte adhesion molecule 1. J Biol Chem 1991;266:2468-2473
4. Li F, Wilkins PP, Crawley S, et al. Post-translatonal modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P-and E-selectin. J Biol Chem. 1996;271;3255-3264
5. Chen XL, Tummala PE, Olliff L, et al. E-selectin gene expression in vascular smooth muscle cells. Circ Res. 1997:80:305-311
6. Benjamin C, Dougas I, Chi-Rosso G, et al. A blocking monoclonal antibody to endothelial-leukocyte adhesion molecule-1(ELAM1). Biochem Biophy Res Commun 1990, 171, 348-3537. Bajt ML. Goodman T and McGuire SL. β2(CD18) mutations abolish ligand recognition by I domain integrins LFA-l(αLβ2, CD11a/CD18) and Mac-1 (αLβ2, CDllb, CD18). J Biol Chem. 1995, 270, 94-98.
8. Skinner MP, Lucas CM, Burns GF, et al. GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem. 1991,266, 5371-5374
9. Wilson RW, Ballantyne CM, Smith CW, et al. Gene targeting yields a CD18-mutant mouse for study of inflammation. J Immunol 1993; 151; 1571-1578.
10. Zimmerman GA, McIntyre TM, Mehra Meena et al. Endothelial cell -associated platelet-activation factor: a novel mechanism for signaling intercellular adhesion. J Cell Biology 1990,110,529-540
11.Spertini O, Kansas GS, Munro JM, et al. Regulation of leukocyte migration by activation of the leukocyte adhesion molecule-1(LAM-1) selection. Nature. 1991, 349, 691-694.
12. Finger EB, Puri KD, Alon R, et al. Adhesion through L-selectin requires a threshold hydrodynamic shear. Nature, 1996, 379, 266-269
13. Lehr HA, Nolte D, Hubner C, et al. Impact of lipid mediator on leukocyte/endothelium interaction. Prog Appl Microcirc 1991;18;82-90.
14. Arndt H, Palitzsch K-D, Anderson DC, et al. Leucocyte-endothelial cell adhesion in a model of intestinal inflammation. Gut 1995;37;374-379.
15. Kunkel EJ and Ley K. Distinct phenotype of E-selectin-deficient mice, E-selectin is required for slow leukocyte rolling in vivo. Circ Res. 1996; 79:1196-1204
16.Weller A, Isenmann S and Vestweber D. Cloning of the mouse endothelial selectins, expression of both E- and P-selectin is inducible by tumor necrosis factor. J Biol Chem 1992, 267, 15176-15183
17. Maier JAM, Statuto M and Ragnotti G, Senescence stimulates U937-Endothelial cell interactions. Exp. Cell Res 1993;208, 270-274.
18. Weber C, Erl W, Pietsch A, et al, Aspirin inhibits nuclear factor-kB mobilization and monocyte adhesion in stimulated human endothelial cells. Circulation 1995;91;1914-1917,.
19. Huang ZH, Bates EJ, Ferrante JV et al. Inhibition of stimulus-induced endothelial cell intercellular adhesion molecule-1, E-selectin, and vascular cellular adhesion molecule-1 expression by arachidonic acid and its hydroxy and hydroperoxy derivatives. Circ Res. 1997; 80: 149-158
20. Minter AJ, Keoshkerian E, Chesterman CN et al. Fibroblast growth factor and heparin protect endothelial cells from the effects of interleukin 1. J Cell Physiol. 1996:167:229-237