籽鹅血清中FSH、LH和PRL与卵巢相应受体mRNA表达的关系
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摘要
为探讨籽鹅在发育和产蛋期间血清中FSH、LH和PRL浓度与卵巢中相应受体基因mRNA表达的相关性,揭示垂体组织中FSH、LH和PRL基因及卵巢中相应受体基因mRNA表达规律,进而了解卵巢受体对FSH、LH和PRL的反应能力。本试验选取1日龄,1、2、3、4、5、6、7和8月龄籽鹅各6只,分别采取血液、垂体和卵巢,利用电化学发光法测定各月龄籽鹅血清中FSH、LH和PRL浓度,用real-time PCR技术对垂体中FSH、LH和PRL基因和卵巢中的相应受体基因mRNA表达进行定量研究。
结果表明：1日龄籽鹅血清中就可检测到FSH、LH和PRL,随着月龄的增加,FSH和LH都呈现出波动性增高,但各月龄之间差异不显著(P>0.05)；垂体中FSH和LH基因及卵巢中受体基因在籽鹅出雏当日有表达,并随着月龄的变化而呈现波动性增加,在性成熟后都表现出上调的趋势,上调趋势一直延续到产蛋期,产蛋期FSH和LH基因及受体基因mRNA的表达量与性成熟前各月龄差异显著(P<0.05)；垂体中PRL基因及卵巢PRL受体基因mRNA在1日龄时表达量较高,从1月龄开始PRL基因的表达量持续下调,下调趋势持续到4月龄,到性成熟后开始上调延续到产蛋期,1-7月龄PRL基因的表达量均低于1日龄,1日龄与8月龄之间差异不显著(P>0.05),但与其它各月龄差异极显著(P<0.01)。通过对血清中三种激素的浓度分别与垂体中相关基因及卵巢中受体基因的表达量进行相关性分析,得出激素与垂体相关基因、激素与卵巢受体基因均呈现出强相关(r2>0.9)。
结果显示：卵巢在发育和排卵过程中对FSH和LH的反应能力较强,FSH和LH对卵巢的发育起到积极的调控作用；而PRL基因及其受体基因的表达变化表明,PRL在卵巢发育过程中尚不能证明具有促进作用。
The purpose of this research is to elucidate the developmental regulation of the concentrations of FSH, LH and PRL in serum of Zi geese during the developmental and egg-laying stages and the expression of follicle stimulating hormone (FSH), luteinizing hormone (LH) and prolactin (PRL) mRNA in pituitaries and their corresponding receptors mRNA in ovaries.It revealed the corresponding rules of FSH, LH and PRL gene and ovarian receptor gene mRNA expression for Further understanding of ovarian receptors in FSH, LH and PRL responsiveness. The expression of FSH, LH and PRL mRNA in pituitaries and their receptors mRNA in ovaries were accessed by quantitative real-time (qRT-PCR) at 1 day and 1,2,3,4,5,6,7 and 8 months of age (n= 6, respectively). The concentrations of FSH, LH and PRL in serum were also examined by using electrochemiluminescence.
The results showed that the concentrations of FSH and LH increased fluctuatly from the newborn to 8 months old (P> 0.05). The concentration of PRL was only examined in one-day-old Zi geese, which was 0.037 mIU/mL. The level of expression of FSH and LH mRNA in pituitaries and their receptors mRNA in ovaries increased fluctuantly until egg-laying stage. The level of expression of FSH, follicle stimulating hormone receptor (FSHR), LH and luteinizing hormone (LHR) mRNA increased factuantly as months of age passed. It showed an upward trend after sexual maturation, continuing into the egg-laying period. The level of expression of FSH, FSHR, LH and LHR mRNA during egg-laying period differed significantly from that before sexual maturation of each month-old goose (P< 0.05). The level of expression of PRL and prolactin (PRLR) mRNA decreased fluctuantly from 1 to 4-month-old,and increased after sexual maturation. The amount of expression of PRL and PRLR mRNA from 1 to 7-month-old geese were all less than that of 1-day-old geese (P> 0.05). The level of expression of PRL and PRLR mRNA at 8-month-old was higher than that of 1-day-old (P> 0.05), and it was extremely higher than that of 1 to 7-month-old (P< 0.01). The correlation analysis was respectively conducted between concentrations of three hormones in serum and related genes in pituitary and receptor gene expression in ovarian to show that the pituitary hormone gene, and ovarian hormone receptor gene were strong correlation(r2>0.9).
The results showed that the response ability of the ovary to FSH and LH in the process of development and ovulation was significant, and FSH and LH play positive role to regulate ovarian development.The PRL gene and the expression of its receptor gene shows that PRL in the ovary during development is not yet proven to be promoted.
结果表明：1日龄籽鹅血清中就可检测到FSH、LH和PRL,随着月龄的增加,FSH和LH都呈现出波动性增高,但各月龄之间差异不显著(P>0.05)；垂体中FSH和LH基因及卵巢中受体基因在籽鹅出雏当日有表达,并随着月龄的变化而呈现波动性增加,在性成熟后都表现出上调的趋势,上调趋势一直延续到产蛋期,产蛋期FSH和LH基因及受体基因mRNA的表达量与性成熟前各月龄差异显著(P<0.05)；垂体中PRL基因及卵巢PRL受体基因mRNA在1日龄时表达量较高,从1月龄开始PRL基因的表达量持续下调,下调趋势持续到4月龄,到性成熟后开始上调延续到产蛋期,1-7月龄PRL基因的表达量均低于1日龄,1日龄与8月龄之间差异不显著(P>0.05),但与其它各月龄差异极显著(P<0.01)。通过对血清中三种激素的浓度分别与垂体中相关基因及卵巢中受体基因的表达量进行相关性分析,得出激素与垂体相关基因、激素与卵巢受体基因均呈现出强相关(r2>0.9)。
结果显示：卵巢在发育和排卵过程中对FSH和LH的反应能力较强,FSH和LH对卵巢的发育起到积极的调控作用；而PRL基因及其受体基因的表达变化表明,PRL在卵巢发育过程中尚不能证明具有促进作用。
The purpose of this research is to elucidate the developmental regulation of the concentrations of FSH, LH and PRL in serum of Zi geese during the developmental and egg-laying stages and the expression of follicle stimulating hormone (FSH), luteinizing hormone (LH) and prolactin (PRL) mRNA in pituitaries and their corresponding receptors mRNA in ovaries.It revealed the corresponding rules of FSH, LH and PRL gene and ovarian receptor gene mRNA expression for Further understanding of ovarian receptors in FSH, LH and PRL responsiveness. The expression of FSH, LH and PRL mRNA in pituitaries and their receptors mRNA in ovaries were accessed by quantitative real-time (qRT-PCR) at 1 day and 1,2,3,4,5,6,7 and 8 months of age (n= 6, respectively). The concentrations of FSH, LH and PRL in serum were also examined by using electrochemiluminescence.
The results showed that the concentrations of FSH and LH increased fluctuatly from the newborn to 8 months old (P> 0.05). The concentration of PRL was only examined in one-day-old Zi geese, which was 0.037 mIU/mL. The level of expression of FSH and LH mRNA in pituitaries and their receptors mRNA in ovaries increased fluctuantly until egg-laying stage. The level of expression of FSH, follicle stimulating hormone receptor (FSHR), LH and luteinizing hormone (LHR) mRNA increased factuantly as months of age passed. It showed an upward trend after sexual maturation, continuing into the egg-laying period. The level of expression of FSH, FSHR, LH and LHR mRNA during egg-laying period differed significantly from that before sexual maturation of each month-old goose (P< 0.05). The level of expression of PRL and prolactin (PRLR) mRNA decreased fluctuantly from 1 to 4-month-old,and increased after sexual maturation. The amount of expression of PRL and PRLR mRNA from 1 to 7-month-old geese were all less than that of 1-day-old geese (P> 0.05). The level of expression of PRL and PRLR mRNA at 8-month-old was higher than that of 1-day-old (P> 0.05), and it was extremely higher than that of 1 to 7-month-old (P< 0.01). The correlation analysis was respectively conducted between concentrations of three hormones in serum and related genes in pituitary and receptor gene expression in ovarian to show that the pituitary hormone gene, and ovarian hormone receptor gene were strong correlation(r2>0.9).
The results showed that the response ability of the ovary to FSH and LH in the process of development and ovulation was significant, and FSH and LH play positive role to regulate ovarian development.The PRL gene and the expression of its receptor gene shows that PRL in the ovary during development is not yet proven to be promoted.
引文
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