P27与NF-κB在子宫内膜异位症中的表达及其意义
摘要
目的:检测NF-κB和P27在子宫内膜异位症(Endometriosis,EMs)中的表达及其相关性,探讨NF-κB和P27在子宫内膜异位症发病中的作用。
方法:采用免疫组织化学法(IHC)检测EMs组(异位内膜组30例和在位内膜组25例),及正常对照组(30例)中NF-κB和P27的表达情况。
结果:NF-κB在EMs组(异位内膜组和在位内膜组)中的表达以强阳性和阳性为主,而在正常对照组中的表达以阴性和弱阳性为主。EMs组(异位内膜组和在位内膜组)NF-κB的相对表达量明显高于正常对照组,差异有统计学意义(P＜0.05);异位内膜组NF-κB的相对表达量高于在位内膜组,差异有统计学意义(P＜0.05);在位内膜组及正常对照组分泌期内膜组织中NF-κB的表达量均高于增生期,差异有统计学意义(P＜0.05)。
P27在EMs组(异位内膜组和在位内膜组)中的表达以阴性和弱阳性为主,而在正常对照组子宫内膜中的表达以阳性为主。EMs组(异位内膜组和在位内膜组)P27的相对表达量明显低于正常对照组,差异有统计学意义(P＜0.001);异位内膜组P27的相对表达量明显低于在位内膜组,差异有统计学意义(P＜0.001);正常对照组分泌期内膜腺上皮细胞P27的表达量高于增生期,差异有统计学意义(P＜0.05);在位内膜组分泌期与增生期内膜组织中P27的表达量无明显差异,差异无统计学意义(P＞0.05)。
NF-κB与P27在EMs组(异位内膜组和在位内膜组)及正常对照组子宫内膜的表达量呈负相关,相关系数为-0.927,相关分析具有统计学意义(P＜0.05)。
结论:
1.NF-κB的表达上调和P27的表达下调可能与EMs的发病有关。
2.NF-κB与P27可能协同参与了EMs的发病。
Objective:To investigate the expression and relevance of NF-κB and P27 in endometriosis for exploring the role of NF-κB and P27 in the pathogenesis of endometriosis.
Methods:Immunohistochemistry was used to detect the expression of NF-κB and P27 in the endometriosis group,including the ectopic endometrium from 30 cases with endometriosis(ectopic endometrium group)and the eutopic endometrium from 25 cases with endometriosis(eutopic endometrium group),and them in the normal endometrium of 30 cases without endometriosis(control group).
Results:The expression of NF-κB in the endometriosis group(ectopic endometrium group and eutopic endometrium group) was highly positive,and it was puny in control group.The increased expression level of NF-?B was seen in the endometriosis group(ectopic endometrium group and eutopic endometrium group)compared with that of in control group.Increased expression of NF-?B was detected in the ectopic endometrium group compared with that of the eutopic endometrium group.The expression of NF-?B in secretory phase was significantly higher than that in proliferative phase in the eutopic group and the control group.
The expression of P27 in the endometriosis group(ectopic endometrium group and eutopic endometrium group)was puny,and it was highly positive in control group.We revealed decreased expression of P27 in the endometriosis group(ectopic endometrium group and eutopic endometrium group)compared with that in control group.Decreased expression of P27 was detected in the ectopic endometrium group compared with that in the eutopic endometrium group.The expression of P27 in secretory phase was slightly higher than that in proliferative phase in the control group.There was no difference between the different menstrual phase of the eutopic group.
The expression of P27 and NF-?B in the endometriosis group(ectopic endometrium group and eutopic endometrium group)and the control group has negative correlation,the correlation coefficient is -0.927.
Conclusions:
1 The up regulation of NF-?B and the down regulation of P27 in the endometriosis suggestes that NF-?B and P27 may play a role in the pathogenesis of endometriosis.
2 The NF-?B and P27 may cause the endometriosis together.
方法:采用免疫组织化学法(IHC)检测EMs组(异位内膜组30例和在位内膜组25例),及正常对照组(30例)中NF-κB和P27的表达情况。
结果:NF-κB在EMs组(异位内膜组和在位内膜组)中的表达以强阳性和阳性为主,而在正常对照组中的表达以阴性和弱阳性为主。EMs组(异位内膜组和在位内膜组)NF-κB的相对表达量明显高于正常对照组,差异有统计学意义(P＜0.05);异位内膜组NF-κB的相对表达量高于在位内膜组,差异有统计学意义(P＜0.05);在位内膜组及正常对照组分泌期内膜组织中NF-κB的表达量均高于增生期,差异有统计学意义(P＜0.05)。
P27在EMs组(异位内膜组和在位内膜组)中的表达以阴性和弱阳性为主,而在正常对照组子宫内膜中的表达以阳性为主。EMs组(异位内膜组和在位内膜组)P27的相对表达量明显低于正常对照组,差异有统计学意义(P＜0.001);异位内膜组P27的相对表达量明显低于在位内膜组,差异有统计学意义(P＜0.001);正常对照组分泌期内膜腺上皮细胞P27的表达量高于增生期,差异有统计学意义(P＜0.05);在位内膜组分泌期与增生期内膜组织中P27的表达量无明显差异,差异无统计学意义(P＞0.05)。
NF-κB与P27在EMs组(异位内膜组和在位内膜组)及正常对照组子宫内膜的表达量呈负相关,相关系数为-0.927,相关分析具有统计学意义(P＜0.05)。
结论:
1.NF-κB的表达上调和P27的表达下调可能与EMs的发病有关。
2.NF-κB与P27可能协同参与了EMs的发病。
Objective:To investigate the expression and relevance of NF-κB and P27 in endometriosis for exploring the role of NF-κB and P27 in the pathogenesis of endometriosis.
Methods:Immunohistochemistry was used to detect the expression of NF-κB and P27 in the endometriosis group,including the ectopic endometrium from 30 cases with endometriosis(ectopic endometrium group)and the eutopic endometrium from 25 cases with endometriosis(eutopic endometrium group),and them in the normal endometrium of 30 cases without endometriosis(control group).
Results:The expression of NF-κB in the endometriosis group(ectopic endometrium group and eutopic endometrium group) was highly positive,and it was puny in control group.The increased expression level of NF-?B was seen in the endometriosis group(ectopic endometrium group and eutopic endometrium group)compared with that of in control group.Increased expression of NF-?B was detected in the ectopic endometrium group compared with that of the eutopic endometrium group.The expression of NF-?B in secretory phase was significantly higher than that in proliferative phase in the eutopic group and the control group.
The expression of P27 in the endometriosis group(ectopic endometrium group and eutopic endometrium group)was puny,and it was highly positive in control group.We revealed decreased expression of P27 in the endometriosis group(ectopic endometrium group and eutopic endometrium group)compared with that in control group.Decreased expression of P27 was detected in the ectopic endometrium group compared with that in the eutopic endometrium group.The expression of P27 in secretory phase was slightly higher than that in proliferative phase in the control group.There was no difference between the different menstrual phase of the eutopic group.
The expression of P27 and NF-?B in the endometriosis group(ectopic endometrium group and eutopic endometrium group)and the control group has negative correlation,the correlation coefficient is -0.927.
Conclusions:
1 The up regulation of NF-?B and the down regulation of P27 in the endometriosis suggestes that NF-?B and P27 may play a role in the pathogenesis of endometriosis.
2 The NF-?B and P27 may cause the endometriosis together.
引文
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[1]乐杰 主编.妇产科学,人民卫生出版社,北京,2005年6月第6版
[2]Pietenpol JA,Bohlander SK,Sato Y,et al.Assignment of the human p27Kip1gene to 12p13 and its analysis in leukemias.Cancer Res,1995,55(6):1206-1210
[3]李朝争,张祥宏.P27、Skp2与肿瘤.社区医学杂志,2007,11(5):64-67
[4]Kawamata N,Morosetti R,Miller CW,et al.Molecular analysis of the cyclin-dependent kinase inhibitor gene p27/kip1 in human malignancies.Cancer Res,1995,55(11):2266-2269
[5]Cho S,Kim JH,Back SH,et al.Polypyrimidine tract-binding protein enhances the internal ribosomal entry site-dependent translation of p27Kip1 mRNA and modulates transition from G1 to S phase.Mol Cell Biol,2005,25(4):1283-1297
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