不同产地蜂胶的质量评价研究
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摘要
蜂胶为蜜蜂科昆虫意大利蜂Apis mellifera L.的干燥分泌物[l]。现代药理学研究表明蜂胶具有抗菌消炎、调节免疫、抗氧化、加速组织愈合等作用。临床上用于高脂血症和糖尿病的辅助治疗,目前蜂胶已广泛地应用于医药、保健品、化妆品等领域。
本文首先对不同产地蜂胶的质量的进行了初步评价。根据《中国药典》2005版收载的蜂胶项下的规定,对13个不同产地的蜂胶分别进行了薄层鉴别以及干燥失重、总灰分、酸不溶性灰分、氧化时间、醇浸出物检查。采用HPLC法测定了13个产地蜂胶中的白杨素及高良姜素的含量,同时采用紫外分光光度法测定了蜂胶总黄酮的含量。结果表明,吉林省蛟河及河南省长葛地区的蜂胶品质较优,同时提示,不同产地的蜂胶质量存在较大差异。
本文采用HPLC-DAD法对蜂胶进行指纹图谱研究,采用Agilent Zorbax SB-C18 (250mm×4.6mm, 5μm)色谱柱,以甲醇-0.1%甲酸为流动相梯度洗脱,检测波长为290nm;流速为0.8ml/min,得到分离度较好的蜂胶指纹图谱,以期为蜂胶全面质量控制提供参考。
采用液质联用技术对蜂胶指纹图谱进行了定性分析,推测了其中18个峰的可能结构,其中9个黄酮类化合物(2个为黄酮类化合物和7个为二氢黄酮类化合物),9个芳香族酸及芳香族酸的酯类化合物(3个为芳香族酸类化合物和6个为芳香族酸的酯类化合物),为蜂胶的药效物质成分提供更多的化学信息,以期为今后能够更加全面、合理的评价蜂胶的质量提供更多的科学依据。
Propolis is dry secretions from the bee Apis mellifera L.of Italian insects.Modern pharmacological studies have shown that propolis has anti-bacterial anti-inflammatory, regulating immunity, anti-oxidation, accelerate tissue healing and so on. Propolis is used adjuvant therapy for hyperlipidemia and diabetes in the clinic. The present propolis has been widely used in medicine, health products, cosmetics and other fields.
In this paper, I have conducted the preliminary evaluation based on the quality of different sources of propolis. According to the 2005 edition of China pharmacopoeia under the provisions of propolis against 13 different origin of propolis were carried out by TLC, as well as loss on drying, total ash, acid insoluble ash, oxidation time, alcohol extract inspection. It were determined using HPLC method in 13 propolis origin chrysin and galangin content, At the same time were determined by ultraviolet spectrophotometry of flavonoids in propolis. The results show that, the propolis in Jilin Province Jiaohe and Henan Province Changge areas are superior to others areas.
In this paper, using HPLC-DAD method on fingerprint of propolis research, using Agilent Zorbax SB-C18 (250mm×4.6mm, 5μm) column with methanol -0.1% formic acid as mobile phase gradient elution; detection wavelength 290nm; velocity To 0.8ml/min,we obtain a better separation of propolis fingerprints with a view to provide reference for total quality control of propolis.
Using LC-MS techniques for propolis fingerprints to qualitativly analyze, speculating the possible structure of 18 peaks, of which 9 flavonoid compounds (2 for the flavonoids and 7 for the dihydro-flavonoids), 9 aromatic acid and aromatic acid esters (3 for the aromatic acids and 6 for the aromatic acid esters), providing more chemical information for the efficacy material composition of propolis, with a view to the future will be more comprehensive and rational evaluation of the quality of propolis to provide more scientific basis.
本文首先对不同产地蜂胶的质量的进行了初步评价。根据《中国药典》2005版收载的蜂胶项下的规定,对13个不同产地的蜂胶分别进行了薄层鉴别以及干燥失重、总灰分、酸不溶性灰分、氧化时间、醇浸出物检查。采用HPLC法测定了13个产地蜂胶中的白杨素及高良姜素的含量,同时采用紫外分光光度法测定了蜂胶总黄酮的含量。结果表明,吉林省蛟河及河南省长葛地区的蜂胶品质较优,同时提示,不同产地的蜂胶质量存在较大差异。
本文采用HPLC-DAD法对蜂胶进行指纹图谱研究,采用Agilent Zorbax SB-C18 (250mm×4.6mm, 5μm)色谱柱,以甲醇-0.1%甲酸为流动相梯度洗脱,检测波长为290nm;流速为0.8ml/min,得到分离度较好的蜂胶指纹图谱,以期为蜂胶全面质量控制提供参考。
采用液质联用技术对蜂胶指纹图谱进行了定性分析,推测了其中18个峰的可能结构,其中9个黄酮类化合物(2个为黄酮类化合物和7个为二氢黄酮类化合物),9个芳香族酸及芳香族酸的酯类化合物(3个为芳香族酸类化合物和6个为芳香族酸的酯类化合物),为蜂胶的药效物质成分提供更多的化学信息,以期为今后能够更加全面、合理的评价蜂胶的质量提供更多的科学依据。
Propolis is dry secretions from the bee Apis mellifera L.of Italian insects.Modern pharmacological studies have shown that propolis has anti-bacterial anti-inflammatory, regulating immunity, anti-oxidation, accelerate tissue healing and so on. Propolis is used adjuvant therapy for hyperlipidemia and diabetes in the clinic. The present propolis has been widely used in medicine, health products, cosmetics and other fields.
In this paper, I have conducted the preliminary evaluation based on the quality of different sources of propolis. According to the 2005 edition of China pharmacopoeia under the provisions of propolis against 13 different origin of propolis were carried out by TLC, as well as loss on drying, total ash, acid insoluble ash, oxidation time, alcohol extract inspection. It were determined using HPLC method in 13 propolis origin chrysin and galangin content, At the same time were determined by ultraviolet spectrophotometry of flavonoids in propolis. The results show that, the propolis in Jilin Province Jiaohe and Henan Province Changge areas are superior to others areas.
In this paper, using HPLC-DAD method on fingerprint of propolis research, using Agilent Zorbax SB-C18 (250mm×4.6mm, 5μm) column with methanol -0.1% formic acid as mobile phase gradient elution; detection wavelength 290nm; velocity To 0.8ml/min,we obtain a better separation of propolis fingerprints with a view to provide reference for total quality control of propolis.
Using LC-MS techniques for propolis fingerprints to qualitativly analyze, speculating the possible structure of 18 peaks, of which 9 flavonoid compounds (2 for the flavonoids and 7 for the dihydro-flavonoids), 9 aromatic acid and aromatic acid esters (3 for the aromatic acids and 6 for the aromatic acid esters), providing more chemical information for the efficacy material composition of propolis, with a view to the future will be more comprehensive and rational evaluation of the quality of propolis to provide more scientific basis.
引文
[1]中华人民共和国国家药典委员会.中华人民共和国药典:一部[Z].北京:化学工业出版社,2005:249.
[2]叶振生.蜂产品深加工技术[M].北京:中国轻工业出版社,2003:274.
[3]蒋三俊.蜂产品对治疗糖尿病的作用[J].特种经济动植物,2003,(8):10.
[4]张敬哲.蜂胶的研究利用概况[J].特种经济动植物,2006,(2):13.
[5]伊和姿,余虹雯,陈瑞华,等.蜂胶在皮肤科的应用[J].上海中医药杂志,1990,(17):28-29.
[6]杨明,隋殿军,孙红,等.蜂胶总黄酮抗心肌缺血作用[J].中草药,2006,37(2):253-255.
[7]于英君,李淑华,郭丽新,等.蜂胶生物学活性与临床应用[J].中医药信息,2002,19(1):27-28.
[8]刘明,倪辉,吴永沛.蜂胶在肝病治疗中的应用[J].中国蜂业,2006,57 (6):27-28.
[9]成玉峰.蜂胶治疗消化性溃疡的临床观察[J].中国医药理论与实践,2005,1(15):75-76.
[10]吴淑兰,李晓光,李明荣,等.蜂胶治疗慢性乙型肝炎的临床应用研究[J].中华临床医药,2002,3 (16):20-21.
[11]王东风,张波,张健,等.蜂胶总黄酮溶液用于烧伤抗渗出作用的实验和临床研究[J].中国临床医药研究杂志,2005,139:15055-15056.
[12]马斌,江珉.蜂胶在空腔内科中临床应用的研究进展[J].西南军医,2006,8(3):72-73.
[13]黄坚.蜂胶治病[J].蜜蜂杂志,2009,(6):28-29.
[14]徐万林著.《中国蜜粉源植物》[M].黑龙江科学技术出版社,1993:35.
[15]王亚群,任永新.蜂胶的化学成分及其保健作用[J].食品与药品,2006,8(12):75-76.
[16] E.L.Ghisalberti.Propolis:a review[J].BeeWorld,1979,60(2):59-84.
[17]郭伽,周立冬.蜂胶的化学成分研究进展[J].中国养蜂,2000,51(2):17-23.
[18] A.L.Tikhonov et al.Composition of a lyophobic phenolic preparation from propolis[J].CA,1978,88(22) :15833n.
[19] M.C.Marcucci.Propolis : chemical composition , biological properties therapeutic activity[J] .Apidolodie,1995,26:83-99.
[20] V.Suchy et al.Study of constituents of propolis of Czecho.slovakian origin I.flavanonand aromatic aeids[J].CA,1982,96(11),82896v.
[21] E.wollenweber et al.Anove1caffeic acid derivative and other constituents of popu1us bud excretion and propolis(bee-glue) .Z.Naturforsch,1987,42c.1030-1034.
[22] E.Nagy et al.Investigation of the chemical constituents,particularly the flavonoids components of propolis and Populi gemma by the GC/MS method[J]. Flavonoids and Bioflavonoids,Elsevier,Amsterdam,The Netherlands,1985,223-240.
[23] W.Greenaway et al.The analysis of bud exudates of Populus x euramericana,and of propolis,by gas chromatography-mass spectrometry[J].Proe.R.Soe.Lond, 1987,B232:249-272.
[24] V.S.Bankova et al.A study of flavonoids of Propolis[J].Nat.Products,1983,46(4):471-474.
[25] S.Mochida et al.Chemical constituents and anti-microbial activity of propolis[J]. Beekeeping Congr,1985,30:455-456.
[26]黄文诚.蜂胶研究进展:化学成分及植物源[J] .中国养蜂,2002,52(6):39-53.
[27] Y,Maruyama,Mishima S,Polyphenols from propolis extracts asantihypertensive agents,Jpn.Kokai Tokkyo Koho 2004,19.
[28] E.L.Ghisalberti et al.Constituents of propolis[J] . Experientia,1978,34(2):157-158.
[29] S.Mochida et al.Chemical constituents and antimicrobial activity of Japanese propolis[J]Beekeeping Congr,1985,455-456.
[30] W.Greenaway et al.Identification by gas chromatography-mass spectrometry of 150 compounds in propolis[J].Z.Naturforsch,1991,46c:111-121.
[31] Mohammadzadeh Shiva,Shariatpanahi Mohammad,Hamedi Manoocherhr.Chemical composition.oral toxicity and antimicrobial activitity of Iranian propolis[J].Food Chemistry,2007,103(4):1097-1103.
[32] C.Garcia-Viguera et al.Study of Canadian propolis by GC/MS and HPLC. [J].Naturforsch,1993,48c:731-735.
[33] Gardana,Claudio;Scaglianti,Martina;Pietta,Piergiorgio et al . Analysis of thepolyphenolic fraction of propolis from different sources by liquid chromatography tandem massspectromtry[J].Journal of Pharmaceutical and Bilmedical Analysis,2007,45(3):390-399.
[34] V.S.Bankovaetal.Isopentenyl cinnamates from poplar buds and propolis[J]. Phytochemistry,1989,28(3):871-873.
[35] M.Vanhaelen et al.PropoliseⅡ.Identification by high-performance chromatography of the rconstituents,bioautograph of the chromatograms of the antibacterial constituents[J]. CA,1980,92(18),153219c.
[36] Velikova M,Bankova V,Marcucci MC.Chemical composition and biological activity of propolis from Brazilian meliponinae.Zeitschrift fur Naturforschung[J].C, Journal of Biosciences,2000,55(9-10):785-789.
[37] Serra Bonvehi Josep,Ventura Coll Francesc,Escola Jorda Rossend.The composition,active components and bacteriostatic activity of propolis in dietetics[J].Journal of the American Oil Chemisis’Society.1994,71(5):529-532.
[38] W.Maciejewiczetal.Gas chromatography-mass spectrometry stuty of propolis:analysis of B-steroids[J].CA,1983,99(10):76939c.
[39] Christov Roumen,Trusheva Boryana,Popova Milena.Chemical composition of propolis from Canada,its antiradical activity and plant origin.Natural Product Research,Part A:structure and Synthesis.2006,20(6):531-536.
[40] Bankova,V,Christov,R,Kujumgiev,A.Chemical composition and antibacterial activity of Brazilian propolis[J].Zeitschrift fuer Naturforschung,C:Biosciences 1995,50(3):167-172.
[41] W.Greenawayetal.Headspace volatiles from propolis[J].Flavour.and Fragr,1989,4:173-175.
[42] S.A.Popravko et al.Derivatives of unsaturated aromatic alcohols in propolis and gum benzoin.CA,1984,101(11),87463d
[43] V.S.Bankova et al.Propolis produced in Bularia and Mongolia:phenoic compounds and plant origin[J].Apidologie,1992,23:79-85.
[44] G.Clair et al.The study of propolis essential oil[J].CA,1981,95(6):49186.
[45]王秉等.北京蜂胶的化学成分研究[J].中药通报,1988,13(10):37-38.
[46] A.L.Tikhonov et al.Components of a water-soluble polyphenol preparation of propolis[J] .CA,1977,87(5):143-146.
[47]尚大民等.蜂胶化学成分的研究[J].中国养蜂,1984,(4) :20-22.
[48] S.A.Popravko et al.Derivatives of unsaturated aromatic alcohols in propolis and gum benzoin.CA,1984,101(11),87463d.
[49]迟家平.辽西蜂胶黄酮类化学成分的研究[J].中国药学杂志,1996,31(5) :246-266.
[50]迟家平.辽西蜂胶中新的桂皮酸酯成分的分离与鉴定[J].药学学报,1996,(7) :558.
[51] V.Suchy et al.Study of the substances containe in propolis of Czechoslovakian origin flavone derivatives.CA,1986,104(11),85461g.
[52] D.Grunberger et al.Preferential cytotoxity on tumor cells by caffeic acid phenethyl ester isoland from propolis[J].Experientia.1988,44:230-232.
[53] S.A.Popravko et al.New natural phenolic triglycerides[J].CA,1982,97(17):141692s.
[54]尉辉杰,田玉慧,李志春.蜂胶黄酮类提取物对去卵巢大鼠骨代谢的影响[J].新乡医学院学报,2005,22(3):218
[55]张波,王东风,王爽.蜂胶总黄酮镇痛作用及其机制研究[J].中国药房,2005, 19(16):1458-1459.
[56]王南舟.蜂胶制剂镇痛抗炎作用初探[J].蜜蜂杂志,2004(5):3-5.
[57]任竣蛾,宋爱青,蜂胶对实验肿瘤细胞培养作用的观察[J].蜜蜂杂志,1992(9):3-5.
[58]郭秀婵,叶梁,张永利.国产蜂胶对肝癌细胞体外杀伤作用的研究[J].中国肿瘤,2002, 7(11):431-432.
[59]李淑华,于晓红,于英君,等.蜂胶对免疫功能低下模型鼠细胞免疫功能的影响[J].中国药理学报,2001,29(3):38-39.
[60]张宝康,王德云,孔祥峰,等.蜂胶黄酮增强免疫作用的研究[J].扬州大学学报,2006,27(3):20-22.
[61] Kujumgiev A,Tsvetkova I,Serkedjieva V,et a1.Antibacterial,antifungal and antiviral activity of propolis of different geographic origin[J].J.Ethmopharmacol,1999.64(3):235-236.
[62]张天宝,黄晓波,岳木生.蜂胶复方消毒剂杀菌效果与毒性的试验观察[J].中国消毒学,2000,17(4):203-206.
[63]刘富海,许正鼎.神奇的蜂胶[M].呼和浩特:内蒙古教育出版社,1999:62-69.
[64]钟立人,韩文辉,张燕萍.蜂胶抗氧化性能的研究[J].中草药,2002,9(33):803-804.
[65]祁雪,李震,罗桂贤,等.蜂胶的化学成分及药理作用研究[J].长春中医学院学报,2001,17(4):60.
[66]李光勇,郭艳萍,张涛,等.蜂胶对二甲基亚硝胺诱导的大鼠肝纤维化的作用[J].现代生物医学进展,2009,11(9):70
[67]潘建国.试论蜂胶调节血脂的机理与新进展[J].蜜蜂杂志,2004(8):25.
[68]吴粹文,强复兴,方兵兵,等.真理蜂胶营养液调节血液作用的研究[J].中国养蜂,1998 (49 ):241.
[69] Hu FL ,Xuan H Z,Chen M L,et a1.Effects of propolis on diabetes millitusin SD rats[J].J Zhejiang Univ:Agric Life Sci(浙江大学学报:农业与生命科学版),2004,30(2):205-209.
[70]郭芳彬.蜂胶对糖尿病的疗效与作用机理探析[J].养蜂科技,2004,(5):325
[71]沙娜,黄慧莲,李萍,等.蜂胶的HPLC指纹图谱研究[J].中国药科大学学报,2009,14(2):144-146.
[72]白雪,于丽云.色谱法在中药指纹图谱中的应用[J].中国老年保健医学,2009,2(7):37-38.
[73]蔡宝昌,刘训红.常用中药材HPLC指纹图谱测定技术[M].北京:化学工业出版社, 2005.
[74] Alexandra C.H.F.Sawaya,Daniela M.Tomazela,Marcos N.Eberlin et a1. Electrospray ionization mass spectrometry fingerprinting of propolis[J].Analyst , 2004 , 129, 739-744.
[2]叶振生.蜂产品深加工技术[M].北京:中国轻工业出版社,2003:274.
[3]蒋三俊.蜂产品对治疗糖尿病的作用[J].特种经济动植物,2003,(8):10.
[4]张敬哲.蜂胶的研究利用概况[J].特种经济动植物,2006,(2):13.
[5]伊和姿,余虹雯,陈瑞华,等.蜂胶在皮肤科的应用[J].上海中医药杂志,1990,(17):28-29.
[6]杨明,隋殿军,孙红,等.蜂胶总黄酮抗心肌缺血作用[J].中草药,2006,37(2):253-255.
[7]于英君,李淑华,郭丽新,等.蜂胶生物学活性与临床应用[J].中医药信息,2002,19(1):27-28.
[8]刘明,倪辉,吴永沛.蜂胶在肝病治疗中的应用[J].中国蜂业,2006,57 (6):27-28.
[9]成玉峰.蜂胶治疗消化性溃疡的临床观察[J].中国医药理论与实践,2005,1(15):75-76.
[10]吴淑兰,李晓光,李明荣,等.蜂胶治疗慢性乙型肝炎的临床应用研究[J].中华临床医药,2002,3 (16):20-21.
[11]王东风,张波,张健,等.蜂胶总黄酮溶液用于烧伤抗渗出作用的实验和临床研究[J].中国临床医药研究杂志,2005,139:15055-15056.
[12]马斌,江珉.蜂胶在空腔内科中临床应用的研究进展[J].西南军医,2006,8(3):72-73.
[13]黄坚.蜂胶治病[J].蜜蜂杂志,2009,(6):28-29.
[14]徐万林著.《中国蜜粉源植物》[M].黑龙江科学技术出版社,1993:35.
[15]王亚群,任永新.蜂胶的化学成分及其保健作用[J].食品与药品,2006,8(12):75-76.
[16] E.L.Ghisalberti.Propolis:a review[J].BeeWorld,1979,60(2):59-84.
[17]郭伽,周立冬.蜂胶的化学成分研究进展[J].中国养蜂,2000,51(2):17-23.
[18] A.L.Tikhonov et al.Composition of a lyophobic phenolic preparation from propolis[J].CA,1978,88(22) :15833n.
[19] M.C.Marcucci.Propolis : chemical composition , biological properties therapeutic activity[J] .Apidolodie,1995,26:83-99.
[20] V.Suchy et al.Study of constituents of propolis of Czecho.slovakian origin I.flavanonand aromatic aeids[J].CA,1982,96(11),82896v.
[21] E.wollenweber et al.Anove1caffeic acid derivative and other constituents of popu1us bud excretion and propolis(bee-glue) .Z.Naturforsch,1987,42c.1030-1034.
[22] E.Nagy et al.Investigation of the chemical constituents,particularly the flavonoids components of propolis and Populi gemma by the GC/MS method[J]. Flavonoids and Bioflavonoids,Elsevier,Amsterdam,The Netherlands,1985,223-240.
[23] W.Greenaway et al.The analysis of bud exudates of Populus x euramericana,and of propolis,by gas chromatography-mass spectrometry[J].Proe.R.Soe.Lond, 1987,B232:249-272.
[24] V.S.Bankova et al.A study of flavonoids of Propolis[J].Nat.Products,1983,46(4):471-474.
[25] S.Mochida et al.Chemical constituents and anti-microbial activity of propolis[J]. Beekeeping Congr,1985,30:455-456.
[26]黄文诚.蜂胶研究进展:化学成分及植物源[J] .中国养蜂,2002,52(6):39-53.
[27] Y,Maruyama,Mishima S,Polyphenols from propolis extracts asantihypertensive agents,Jpn.Kokai Tokkyo Koho 2004,19.
[28] E.L.Ghisalberti et al.Constituents of propolis[J] . Experientia,1978,34(2):157-158.
[29] S.Mochida et al.Chemical constituents and antimicrobial activity of Japanese propolis[J]Beekeeping Congr,1985,455-456.
[30] W.Greenaway et al.Identification by gas chromatography-mass spectrometry of 150 compounds in propolis[J].Z.Naturforsch,1991,46c:111-121.
[31] Mohammadzadeh Shiva,Shariatpanahi Mohammad,Hamedi Manoocherhr.Chemical composition.oral toxicity and antimicrobial activitity of Iranian propolis[J].Food Chemistry,2007,103(4):1097-1103.
[32] C.Garcia-Viguera et al.Study of Canadian propolis by GC/MS and HPLC. [J].Naturforsch,1993,48c:731-735.
[33] Gardana,Claudio;Scaglianti,Martina;Pietta,Piergiorgio et al . Analysis of thepolyphenolic fraction of propolis from different sources by liquid chromatography tandem massspectromtry[J].Journal of Pharmaceutical and Bilmedical Analysis,2007,45(3):390-399.
[34] V.S.Bankovaetal.Isopentenyl cinnamates from poplar buds and propolis[J]. Phytochemistry,1989,28(3):871-873.
[35] M.Vanhaelen et al.PropoliseⅡ.Identification by high-performance chromatography of the rconstituents,bioautograph of the chromatograms of the antibacterial constituents[J]. CA,1980,92(18),153219c.
[36] Velikova M,Bankova V,Marcucci MC.Chemical composition and biological activity of propolis from Brazilian meliponinae.Zeitschrift fur Naturforschung[J].C, Journal of Biosciences,2000,55(9-10):785-789.
[37] Serra Bonvehi Josep,Ventura Coll Francesc,Escola Jorda Rossend.The composition,active components and bacteriostatic activity of propolis in dietetics[J].Journal of the American Oil Chemisis’Society.1994,71(5):529-532.
[38] W.Maciejewiczetal.Gas chromatography-mass spectrometry stuty of propolis:analysis of B-steroids[J].CA,1983,99(10):76939c.
[39] Christov Roumen,Trusheva Boryana,Popova Milena.Chemical composition of propolis from Canada,its antiradical activity and plant origin.Natural Product Research,Part A:structure and Synthesis.2006,20(6):531-536.
[40] Bankova,V,Christov,R,Kujumgiev,A.Chemical composition and antibacterial activity of Brazilian propolis[J].Zeitschrift fuer Naturforschung,C:Biosciences 1995,50(3):167-172.
[41] W.Greenawayetal.Headspace volatiles from propolis[J].Flavour.and Fragr,1989,4:173-175.
[42] S.A.Popravko et al.Derivatives of unsaturated aromatic alcohols in propolis and gum benzoin.CA,1984,101(11),87463d
[43] V.S.Bankova et al.Propolis produced in Bularia and Mongolia:phenoic compounds and plant origin[J].Apidologie,1992,23:79-85.
[44] G.Clair et al.The study of propolis essential oil[J].CA,1981,95(6):49186.
[45]王秉等.北京蜂胶的化学成分研究[J].中药通报,1988,13(10):37-38.
[46] A.L.Tikhonov et al.Components of a water-soluble polyphenol preparation of propolis[J] .CA,1977,87(5):143-146.
[47]尚大民等.蜂胶化学成分的研究[J].中国养蜂,1984,(4) :20-22.
[48] S.A.Popravko et al.Derivatives of unsaturated aromatic alcohols in propolis and gum benzoin.CA,1984,101(11),87463d.
[49]迟家平.辽西蜂胶黄酮类化学成分的研究[J].中国药学杂志,1996,31(5) :246-266.
[50]迟家平.辽西蜂胶中新的桂皮酸酯成分的分离与鉴定[J].药学学报,1996,(7) :558.
[51] V.Suchy et al.Study of the substances containe in propolis of Czechoslovakian origin flavone derivatives.CA,1986,104(11),85461g.
[52] D.Grunberger et al.Preferential cytotoxity on tumor cells by caffeic acid phenethyl ester isoland from propolis[J].Experientia.1988,44:230-232.
[53] S.A.Popravko et al.New natural phenolic triglycerides[J].CA,1982,97(17):141692s.
[54]尉辉杰,田玉慧,李志春.蜂胶黄酮类提取物对去卵巢大鼠骨代谢的影响[J].新乡医学院学报,2005,22(3):218
[55]张波,王东风,王爽.蜂胶总黄酮镇痛作用及其机制研究[J].中国药房,2005, 19(16):1458-1459.
[56]王南舟.蜂胶制剂镇痛抗炎作用初探[J].蜜蜂杂志,2004(5):3-5.
[57]任竣蛾,宋爱青,蜂胶对实验肿瘤细胞培养作用的观察[J].蜜蜂杂志,1992(9):3-5.
[58]郭秀婵,叶梁,张永利.国产蜂胶对肝癌细胞体外杀伤作用的研究[J].中国肿瘤,2002, 7(11):431-432.
[59]李淑华,于晓红,于英君,等.蜂胶对免疫功能低下模型鼠细胞免疫功能的影响[J].中国药理学报,2001,29(3):38-39.
[60]张宝康,王德云,孔祥峰,等.蜂胶黄酮增强免疫作用的研究[J].扬州大学学报,2006,27(3):20-22.
[61] Kujumgiev A,Tsvetkova I,Serkedjieva V,et a1.Antibacterial,antifungal and antiviral activity of propolis of different geographic origin[J].J.Ethmopharmacol,1999.64(3):235-236.
[62]张天宝,黄晓波,岳木生.蜂胶复方消毒剂杀菌效果与毒性的试验观察[J].中国消毒学,2000,17(4):203-206.
[63]刘富海,许正鼎.神奇的蜂胶[M].呼和浩特:内蒙古教育出版社,1999:62-69.
[64]钟立人,韩文辉,张燕萍.蜂胶抗氧化性能的研究[J].中草药,2002,9(33):803-804.
[65]祁雪,李震,罗桂贤,等.蜂胶的化学成分及药理作用研究[J].长春中医学院学报,2001,17(4):60.
[66]李光勇,郭艳萍,张涛,等.蜂胶对二甲基亚硝胺诱导的大鼠肝纤维化的作用[J].现代生物医学进展,2009,11(9):70
[67]潘建国.试论蜂胶调节血脂的机理与新进展[J].蜜蜂杂志,2004(8):25.
[68]吴粹文,强复兴,方兵兵,等.真理蜂胶营养液调节血液作用的研究[J].中国养蜂,1998 (49 ):241.
[69] Hu FL ,Xuan H Z,Chen M L,et a1.Effects of propolis on diabetes millitusin SD rats[J].J Zhejiang Univ:Agric Life Sci(浙江大学学报:农业与生命科学版),2004,30(2):205-209.
[70]郭芳彬.蜂胶对糖尿病的疗效与作用机理探析[J].养蜂科技,2004,(5):325
[71]沙娜,黄慧莲,李萍,等.蜂胶的HPLC指纹图谱研究[J].中国药科大学学报,2009,14(2):144-146.
[72]白雪,于丽云.色谱法在中药指纹图谱中的应用[J].中国老年保健医学,2009,2(7):37-38.
[73]蔡宝昌,刘训红.常用中药材HPLC指纹图谱测定技术[M].北京:化学工业出版社, 2005.
[74] Alexandra C.H.F.Sawaya,Daniela M.Tomazela,Marcos N.Eberlin et a1. Electrospray ionization mass spectrometry fingerprinting of propolis[J].Analyst , 2004 , 129, 739-744.