虾青素对凡纳滨对虾的免疫防护及对虾养殖的水质监测和WSSV检测
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摘要
将虾青素添加到凡纳滨对虾(Litopenaeus vannamei)配合饲料中,从抗氧化角度入手,在个体、细胞和分子水平研究虾青素对凡纳滨对虾免疫防护和抗氧化酶基因的影响,以期全面评价虾青素在改善凡纳滨对虾的生理功能和提高凡纳滨对虾抗病力等方面的作用,进而推测虾青素的作用机理。
虾青素对感染WSSV的凡纳滨对虾的免疫防护作用。评价指标有：血液免疫指标(血细胞计数、血细胞吞噬百分比、血细胞吞噬指数、血清抗菌活力、血清溶菌活力、血清酚氧化酶活力、血清抗超氧阴离子自由基活力),WSSV感染后的累积死亡率及相对免疫保护率。实验证明：饲料中添加80 mg/kg虾青素可显著提高实验组对虾的血液学指标(p<0.05)；实验组中感染WSSV的凡纳滨对虾累计死亡率为76.3%,免疫保护率为23.7%：
虾青素对凡纳滨对虾抗氧化酶基因转录的影响。采用β-actin作为内参,用半定量PCR法研究添加虾青素作为抗氧化剂对凡纳滨对虾肝胰腺MnSOD、CAT和GPX 3种抗氧化酶基因转录水平的影响。结果表明,饲料中添加80mg/kg虾青素能明显提高抗氧化物酶系统包括SOD、CAT、GPX的mRNA水平(p<0.05)。
针对养殖对虾病害频发的现状,本实验还初步研究了中国明对虾(Fenaeus chinensis)养殖水体理化因子变化与对虾白斑综合症病毒(WSSV)感染率的相关性。通过对养成池的水质监测及对虾WSSV检测,发现在WSSV感染率较高的的8月份,水体溶解氧迅速下降,温度处于WSSV易感的温度范围,pH值变化剧烈,,铵盐和硝酸盐含量骤然升高。这说明恶化的水体环境能增加对虾WSSV的易感性,或使潜伏感染WSSV的对虾产生应激反应引起急性感染死亡
In this paper, the astaxanthin was added to the feedstuff of Litopenaeus vannamei. The effect of astaxanthin upon L. vannamei was studied in terms of anti-oxidation at individual cell and molecular levels so as to give an overall appraisal of astaxanthin used to improve L. vannamei physiological function and resistance to disease. Furthermore the mechanism of astaxanthin action could be predicted. The effect of astaxanthin to L. vannamei is discussed as below:The effect of astaxanthin to L. vannamei after challenged with WSSV; the effect of astaxanthin on transcription activity of anti-oxidase gene in L. vannamei liver. Evaluation factors were selected according to various experiment aims:immunological index in hemolymph(total haemocyte counts, phagocytic percentage of haemocyte, phagocytic index, serum anti-bacterial activity, serum lysozyme activity, serum phonoloxidase activity, serum anti-superoxide radical activity); resistance to disease (the mortality of L. vannamei after challenged with WSSV); index in molecular level(SOD CAT and GPX mRNA leve 1).
The results showed that astaxanthin could increase immunological index in hemol-ymph(p<0.05), the mortality of L. vannamei after challenged with WSSV was 76.3% in the supplementation level of 80 mg/kg group, the relative percent suvrival(RPS)valu e of the test group reached 23.7%. The effect of astaxanhin on gene expression of an ti-oxidase was determined using semiquantitative reverse transcription polymerase chaim reaction(RT-PCR) analysis, the results suggest that astaxanhin can impove the transcri ption acivities of anti-oxidase gene in L. Vannamei liver(p<0.05).
In view of frequent occurrence of epidemic disease in shrimp culture, this paper studies on the relationship between WSSV and environmental factors in shrimp culture ponds of Fenaeus chinensis. By water quality monitoring and WSSV detection, it was shown from the analysis that when WSSV of cultured shrimp outbreak in august, dissolved oxygen in the culture ponds decreased rapidly, the variable ranges of temperature was from 24.3℃to 28.4℃in which F. chinensis can easily suffer from WSSV, pH changed acutely, ammonium and nitrate contents in the water increased suddenly. Apparently, aggravation of water quality can decrease the resistance of F. Chinensis and suffer from WSSV easily, while penaeid acute viremia survivors may die of hyperirritability.
虾青素对感染WSSV的凡纳滨对虾的免疫防护作用。评价指标有：血液免疫指标(血细胞计数、血细胞吞噬百分比、血细胞吞噬指数、血清抗菌活力、血清溶菌活力、血清酚氧化酶活力、血清抗超氧阴离子自由基活力),WSSV感染后的累积死亡率及相对免疫保护率。实验证明：饲料中添加80 mg/kg虾青素可显著提高实验组对虾的血液学指标(p<0.05)；实验组中感染WSSV的凡纳滨对虾累计死亡率为76.3%,免疫保护率为23.7%：
虾青素对凡纳滨对虾抗氧化酶基因转录的影响。采用β-actin作为内参,用半定量PCR法研究添加虾青素作为抗氧化剂对凡纳滨对虾肝胰腺MnSOD、CAT和GPX 3种抗氧化酶基因转录水平的影响。结果表明,饲料中添加80mg/kg虾青素能明显提高抗氧化物酶系统包括SOD、CAT、GPX的mRNA水平(p<0.05)。
针对养殖对虾病害频发的现状,本实验还初步研究了中国明对虾(Fenaeus chinensis)养殖水体理化因子变化与对虾白斑综合症病毒(WSSV)感染率的相关性。通过对养成池的水质监测及对虾WSSV检测,发现在WSSV感染率较高的的8月份,水体溶解氧迅速下降,温度处于WSSV易感的温度范围,pH值变化剧烈,,铵盐和硝酸盐含量骤然升高。这说明恶化的水体环境能增加对虾WSSV的易感性,或使潜伏感染WSSV的对虾产生应激反应引起急性感染死亡
In this paper, the astaxanthin was added to the feedstuff of Litopenaeus vannamei. The effect of astaxanthin upon L. vannamei was studied in terms of anti-oxidation at individual cell and molecular levels so as to give an overall appraisal of astaxanthin used to improve L. vannamei physiological function and resistance to disease. Furthermore the mechanism of astaxanthin action could be predicted. The effect of astaxanthin to L. vannamei is discussed as below:The effect of astaxanthin to L. vannamei after challenged with WSSV; the effect of astaxanthin on transcription activity of anti-oxidase gene in L. vannamei liver. Evaluation factors were selected according to various experiment aims:immunological index in hemolymph(total haemocyte counts, phagocytic percentage of haemocyte, phagocytic index, serum anti-bacterial activity, serum lysozyme activity, serum phonoloxidase activity, serum anti-superoxide radical activity); resistance to disease (the mortality of L. vannamei after challenged with WSSV); index in molecular level(SOD CAT and GPX mRNA leve 1).
The results showed that astaxanthin could increase immunological index in hemol-ymph(p<0.05), the mortality of L. vannamei after challenged with WSSV was 76.3% in the supplementation level of 80 mg/kg group, the relative percent suvrival(RPS)valu e of the test group reached 23.7%. The effect of astaxanhin on gene expression of an ti-oxidase was determined using semiquantitative reverse transcription polymerase chaim reaction(RT-PCR) analysis, the results suggest that astaxanhin can impove the transcri ption acivities of anti-oxidase gene in L. Vannamei liver(p<0.05).
In view of frequent occurrence of epidemic disease in shrimp culture, this paper studies on the relationship between WSSV and environmental factors in shrimp culture ponds of Fenaeus chinensis. By water quality monitoring and WSSV detection, it was shown from the analysis that when WSSV of cultured shrimp outbreak in august, dissolved oxygen in the culture ponds decreased rapidly, the variable ranges of temperature was from 24.3℃to 28.4℃in which F. chinensis can easily suffer from WSSV, pH changed acutely, ammonium and nitrate contents in the water increased suddenly. Apparently, aggravation of water quality can decrease the resistance of F. Chinensis and suffer from WSSV easily, while penaeid acute viremia survivors may die of hyperirritability.
引文
[1]Sakai M. Current reseach status of fish immunostimulants. Aquaeulture,1999,172(1-2):63-92.
[2]李红霞.CpG寡脱氧核苷酸对中华绒鳌蟹免疫功能调节作用的研究.苏州大学硕士学位论文,2007.11.
[3]叶燕玲,陈宽智.中国对虾血细胞超微结构、分类及计数.青岛海洋大学学报,1993,23(2)：35-42.
[4]Soderhall K, Smith V. J, Johansson M. W. Exocytosis and uptake of bacteria by isolated haemocyte populations of two crustaceans:evidence for cellular co-operation in the defence reactions of arthropods. Cell and Tissue Research,1986,245:43-49.
[5]Jenny R. Gilles L.M. State of art of immunological tools and health control of penaeid shrimp. Aquaculture,2000:109-119.
[6]Bayne C.J. Phagocytosis and non-self recognition in invertebrates. Phagocyteosis appears to be an ancient line of defence. BioScience,1990,40:723-731.
[7]徐海圣,徐步进.甲壳动物细胞及体液免疫机理的研究进展.大连水产学报,2001,16(1)：41-44.
[8]刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究.海洋与湖沼,1998,29(2)：113-117.
[9]刘树青,江晓路,牟海津.免疫多糖对日本对虾血清酶活性的研究.中国水产科学,1999,6(3)：11 1-113.
[10]王雷,李光友,毛远兴.中国对虾血淋巴中抗菌、溶菌活力与酚氧化酶活力的测定及其特性研究.海洋与湖沼,1995,26(2)：179-18.
[11]Alexandra A. Response of penaeid shrimp to exposure to Vibrio species. Fish & Shellfish Immunology, 1991, 1(1):59-70.
[12]Destoumieux D, Bulet P, Loew D, et.al. A new family of antimicrobial peptides isolated from the Penaeus vannamei(Decapode). The Journal of Biology Chemistry,1997,272:28398-28406.
[13]庄承纪.壳多糖对罗氏沼虾、斑节对虾苗生长和抗菌防病作用研究.湛江海洋大学学报,1998,1 8(3)：29～34.
[14]曹丹,周洪琪.壳聚糖对异育银鲫的生长、蛋白质合成及肌肉营养成分的影响.淡水渔业,2004,34(1)：6-9.
[15]刘兴国.低分子壳聚糖对罗非鱼肝组织抗氧化能力和肝脂含量影响研究.海洋渔业,2004,26(4)：291-294.
[16]胡品虎.稀土甲壳素在河蟹养殖中的应用.水产养殖,1999,(5)：13～14.
[17]江晓路,刘树青,牟海律等.真菌多糖对中国明对虾血清及淋巴细胞免疫影响.动物学研究,1999,20(1)：41～45.
[18]许国焕.两种多聚糖对彭泽鲫生长影响及免疫促进作用的初步研究.水利渔业,2002,22(4)：49～51.
[19]牟海津,江晓路,刘树青,等.免疫多糖对栉孔扇贝酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶活性的影响.青岛海洋大学学报,1999,25(3)：463～468.
[20]刘树青,江晓路,牟海津,等.免疫多糖对日本对虾血清酶活性的影响.中国水产科学,1999,6(3)：107-109.
[21]刘岩,江晓路,吕青,等.聚甘露糖醛酸对虾免疫相关酶活胜和溶菌溶血活性的影响.水产学报,2000,24(6)：549～553.
[22]刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究.海洋与湖沼,1998,29(2)：113～118.
[23]Chang C.F, Su M.S, Chen H.Y. Vibriosis resistance and wound healing enhancement of Penaeus monodon by beta-1,3-glucan from Schizophyllum commune and polyphosphorylated 1-ascorbic acid. Journal of Taiwan fisheries Research.1996 4:43～54.
[24]谭北平,周歧存,郑石轩等.β-1,3／1,6-葡聚糖制剂对凡纳对虾生长及免疫力的影响.高技术通讯2004.5：73-78.
[25]Itami T, Asano M, Tokushige K, et.al. Enhancement of disease resistance of kuruma shrimp, Peneus japonicus, after oral adminitraion of peptidoglycan derived from Bifibacterium thermophilum. Aquaculture,1998,164:277-288.
[26]孟凡伦,张玉臻.甲壳动物中的酚氧化酶原激活系统研究评价.海洋与湖沼,1999,30(1)：110-115.
[27]王秀华,宋晓玲.肽聚糖制剂对南美白对虾体液免疫因子的影响.中国水产科学,2004,11(1)：26-30.
[28]黎中宝编.饲料添加剂.厦门：厦门大学出版社.2004.
[29]崔青曼,张耀红.中草药、多糖复方添加剂提高河蟹机体免疫力的研究.水利渔业,2001,21(4)：40～41.
[30]罗日祥.中药制剂对中国对虾免疫活性物质的诱导作用.海洋与湖沼,1997,28(6)：573～576.
[31]杜爱芳.复方大蒜油添加剂对中国对虾免疫机能的增强作用.浙江大学学报,1997,23(3)：317～320.
[32]Sirirat R, Sombat R, Somkiat P, et al. Immunity enhancement in black tiger shrimp by a probiont bacterium(Bacillus Sll). Aquaculture,2000,191(4,5):271-288.
[33]蔡刚,王杉霖,李文权等.天然虾青素在水产养殖业中的应用研究进展.台湾海峡,2003.22(3)：377-385.
[34]杨艳.虾青素抗氧化活性机制研究进展.国外医学卫生学分册.2008,35(4)：23 1-233.
[35]张明祥,赵建国.国内外虾青素的研究进展.粮食与饲料工业,2002(1)：26-28.
[36]Lee S H, David B.M. Effects, quenching mechanisms and kinetics of carotenoids in chloroph-yll sensitized photooxidafion of soybean oil. Journal of Agricultural and Food Chemistry,1990, 38:1630-1634.
[37]Chien Y.H., Pan C.H., Hunter B. The resistance to physical stresses by Penaeus monodon juveniles fed diets supplemented with astaxanthin. Aquaculture,2003,216(1-4):177-191.
[38]Pan C.H., Chien Y.H., Hunter B.The resistance to ammonia stress of Penaeus monodon Fabricius juvenile fed diets supplemented with astaxanthin. Journal of Experimental Marine Biology and Ecology,2003,297:107-118.
[39]Wang Y J, Chien Y H, Pan C H. Effects of dietary supplementation of carotenoids on survival, growth, pigmentation, and antioxidant capacity of characins, Hyphessobrycon callistus. Aquaculture,2006, 261:641-648.
[40]Yamada S. Pigmentation of prawn (Penaeus japonicus) with carotenoids:effect of dietary astaxanthin,-carotene and canthaxanthin on pigmentation. Aquaculture,1990,87(3-4):323-330.
[41]Menasveta P, Worawattanamateekul W, Latscha Y, et al. Correction of tiger prawn(Penaeus monodon Fabricus) coloration by astaxanthin. Aquacultural Engineering,1993,12:(4) 203-213.
[42]Merchie, Lavens P. Effect of vitamin C and astaxanthin on stress and disease resistance of postlarval tiger shrimp penaeus monodon (Fabricius). Aquaculture Research,1998,29:579-585.
[43]Chien YH. Shiau W. C. The effects of dietary supplementation of algae and synthetic astaxan thin on body astaxanthin,survival,growth, and low dissolved oxygen stress resistance of kuruma prawn, Marsupenaeus japonicus Bate. Journal of Experimental Marine Biology and Ecology, 2005,318:201-211.
[44]Pan,C.H,Chien YH, Cheng J.H. Effect of light regime, algae in the water, and dietary astaxanthin on pigmentation, growth, and survival of black tiger prawn Penaeus monodon post-larvae. Zoological Studies,2001,40(4):371-382.
[45]潘志弘.影响草虾类胡萝卜素分布因素及虾红素在草虾生物功能之研究.国立台湾海洋大学,2001.
[46]Lutsenko E.A, Carcamo J.M, Golde D.M. Vitamin C prevents DNA mutation induced by oxidative stress.The Journal of Biological Chemistry.2002,277(19):16895-16899.
[47]卫涛涛.生物大分子的氧化损伤与疾病.怀化医专学报,2006,5(1)：81-84.
[48]Schneider M, Diemer K, Engelhart K,et al. Protective effects of vitamins C and E on the number of micronuclei in lymphocytes in smokers and their role in ascorbate free radical formation in plasma. Free Radical Research,2001,34 (3):209-219.
[49]Cetinkaya O, Silig Y, Cetinkaya S,et al. The effects of Rumex patientia extract on rat liver and erythrocyte antioxidant enzyme system. Pharmazie,2002,57(7):487-488.
[50]查锡良.生物化学.北京：人民卫生出版社,2001,168-172.
[51]Fridovich I. Superoxide dismutases:studies of structure and mechanism. Advance Experiment Medicine Biology.1976,74:530-539.
[52]Geret F, Serafim A, Barreira L, et al. Response of antioxidant systems to copper in the gills of the clam Ruditapes decussatus. Marine Environment Research,2002,54(3-5):413-417.
[53]Khachik F, Carvalho L, Bernstein P.S, et al. Chemistry, distribution, and metabolism of tomato carotenoids and their impact on human health. Experimental Biology and Medicine,2002,227 (10): 845-851.
[54]Cheng W, Tung Y H, Chiou T T,et al. Cloning and characterisation of mitochondrial manganese superoxide dismutase (mtMnSOD) from the giant freshwater prawn Macrobrachium rosenbergii.Fish & Shellfish Immunology,2006,21(4):453-466.
[55]Cheng W, Tung Y H, Liu C H, et al. Molecular cloning and characterisation of copper/zinc superoxide dismutase (Cu/ZnSOD) from the giant freshwater prawn Macrobrachium rosenbergii. Fish & Shellfish Immunol,2006,21(1):102-112.
[56]Gomez A.G, Barillas C.V, Peregrino A.B, et al. The cytosolic manganese superoxide dismutase from the shrimp Litopenaeus vannamei:Molecular cloning and expression. Developmental & Comparative Immunology,2006,30 (10):893-900.
[57]Ken C.F, Weng D.F, Duan K.J, et al. Characterization of copper/zinc-superoxide dismutase from Pagrus major cDNA and enzyme stability. Journal of Agricultural Food Chemistry,2002,50(4): 784-789.
[58]Pannunzio T.M, Storey K.B. Antioxidant defenses and lipid peroxidation during anoxia stress and aerobic recovery in the marine gastropod Littorina littorea. Journal of Experimental Marine Biology and Ecology,1998,221(2):277-292.
[59]Willmore W.G, Storey K.B. Antioxidant systems and anoxia tolerance in a freshwater turtle Trachemys scripta elegans. Molecular and Cellular Biochemistry,170(1-2):177-185.
[60]Chetty A.N, Indira K. Protection against free radical toxicity in mantle and foot of a fresh water bivalve exposed to Ambient ammonia. Proceeding of the National Academy of Sciences of India,1994, 64(1):35-40.
[61]白洁,唐学玺.久效磷对中国对虾体内SOD活力影响的初步研究.海洋通报,1998,17(41-45).
[62]Zikic V, Ognjanovic B.I,Pavlovic S.Z, et al. Activities of superoxide dismutase and catalse in erythrocytes and transminasea in the plasma of carps(Cyprinus carpio L.)exposed to cadium Physiology Research,1997,46 (5):391-396.
[63]Doyotte A, Cossu C, Jacquin M C, et al. Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve Unio tumidus. Aquatic Toxicology,1997,39(2):93-110.
[64]Brouwer M B. Biochemical defense mechanisms against copper include oxidative damage in the blue crab,Callinects sapidus. Archives of Biochemistry Biophysics 1998(2):257-264.
[65]Parihar M.S, Javeri T,Hemnani T, et al. Responses of superoxide dimutase,glutathione peroxidase and reduced glutathione antioxidant defense in gills of the fresh water catfish (Heteropneusres fossilis) to short term elevated temperature. Journal of Thermal Biology,1997,22 (2):151-156.
[66]Wilhelm F.D. Fish antioxidant defenses comparative approach. Brazilian Journal of Medical Biological Research,1996,29(12):1735-1742.
[67]牟海津,江晓路,刘树清.免疫多糖对栉孔扇贝酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶活性的影响.青岛海洋大学学报,1999,29(3)：462-468.
[68]刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究.海洋与湖沼,1998,29(2)：113-118.
[69]孟繁伦,马桂容,孔建.养殖中国对虾益生素制品的研究.山东大学学报,1999,33(1)：101-105.
[70]Boutet I, Tanguy A, Moraga D. Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions.Gene,2004,329:147-157.
[71]Labreuche Y, Lambert C, Soudant P, et al. Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32. Microbes and Infection,2006,8(12-13):2715-2724.
[72]Goodall C.P, Bender R.C, Broderick E.J, et al. Constitutive differences in Cu/Zn superoxide d-ismutase mRNA levels and activity in hemocytes of Biomphalaria glabrata(Mollusca)that are either susceptible or resistant to Schistosoma mansoni(Trematoda).Molecular and Biochemical P-arasitology,2004,137(2):321-328.
[73]Zelck U.E, Janje B, Schneider O. Superoxide dismutase expression and H2O2 production by hemocytes of the trematode intermediate host Lymnaea stagnalis(Gastropoda).Developmental & Comparative Immunology,2005,29(4):305-314.
[74]Yuan H.T, Bingle C.D, Kelly F.J. Differential patterns of antioxidant enzyme mRNA expression in guinea pig lung and liver during development. Biochimica BiophysicaActa,1996,1305 (3):163～171.
[75]Gomez-Anduro GA, Barillas-Mury C.V, Peregrino-Urate A.B, et al. The cytosolic manganese superoxide dismutase from the shrimpLitopenaeus vannamei:Molecular cloning and expression. Developmental & Comparative Immunology,2006,30(10):893～900.
[76]Tavares-Sanchez O.L,Gomez-Anduro GA, Felipe-Ortega X,et al.The Catalase from the white shrimp Penaeus(Litopenaeus vannamei):molecular cloning and protein detection. Comparative Biochemistry and Physiology,2004,138(4):331～337.
[77]瞿建宏,胡庚东,吴伟.2006.苯酚胁迫下罗非鱼组织中过氧化氢酶与谷胱甘肽-S-转移酶的动态变化生态环境(4)：687-690.
[78]黄长江,董巧.2006.苯并芘对罗非鱼肝脏抗氧化防御系统的影响.汕头大学学报(自然科学版)(4)：5 1-56.
[79]Dandapat J, Chainy G B, Janardhana Rao K. Dietary vitamin E modulates antioxidant defence system in giant freshwater prawn, Macrobrachium rosenbergii.Comparative Biochemistry and Physiology,2000,127 (1):101-115.
[80]Cheung C.C, Zheng GJ, Li A.M, et al. Relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquatic Toxicology,2001,52(3-4):189-203.
[81]Krishnakumar P.K, Casillas E, Snider R.G, et al.Environmental contaminants and the prevalence of hemic neoplasia(leukemia) in the common mussel(Mytilus edulis complex)from Puget Sound, Washington, U.S.A. Journal of Invertebrate Pathology,1999,73(2):135-46.
[82]Gerhard GS, Kauffman E.J, Grundy M.A. Molecular cloning and sequence analysis of the Danio rerio catalase gene.Comparative Biochemistry and Physiology,2000,127(4):447～457.
[83]孙翰昌,丁诗华.Cu2-中华倒刺鲤抗氧化功能的毒理效应.农业环境科学学报,2006,25(1)：69-72.
[84]Santovito G, Piccinni E, Cassini A, et al. Antioxidant responses of the mediterranean mussel, Mytilus galloprovincialis,to environmental variability of dissolved oxygen. Comparative Biochemistry and Physiology,2005,140(3-4):321-329.
[85]Company R, Serafim A, Bebianno M.J, et al. Effect of cadmium,copper and mercury on antioxidant enzyme activities and lipid peroxidation in the gills of the hydrothermal vent mussel Bathymodiolus azoricus. Marine Environmental Research,2004,58(2-5):377-381.
[86]Fukuhara R, Kageyama T. Tissue distribution, molecular cloning, and gene expression of cytosolic glutathione peroxidase in Japanese monkey. Zoological Science,2003,20(7):861-868.
[87]Singh A.K, Shichi H. A novel glutathione peroxidase in bovine eye Sequence analysis, mRNA level, and translation. The Journal of Biochemistry,1998,273(40):26171-26178.
[88]Knopp E.A, Arndt T.L, Eng K, et al. Murine phospholipid hydroperoxide glutathione peroxidase:cDNA sequence,tissue expression,and mapping. Mammal Genome,1999,10(6):601-605.
[89]倪多娇.栉孔扇贝参与氧化反应基因的克隆与表达分析.中国科学院海洋研究所博士学位论文,2007：87.
[90]许雅香,许梓荣,朱玉芳.对虾白斑综合征病毒分子生物学研究进展Marine Sciences,2004,28 (12):63-65.
[91]吴信忠.中国海洋病害主流研究的进展.太平洋学报,2005,10：49-59.
[92]孙成波,何建国,黎子兰等.凡纳滨对虾和斑节对虾对WSSV敏感性的比较.湛江海洋大学学报,2006,26(3)：17-20.
[93]张立人,张建红,陈棣华等.东方对虾杆状病毒在宿主细胞内装配.电子显微镜学报,1994,13(5)354.
[94]Van H.M,Wittteveldt J, Peters S, et al. The white spot syndrome virus DNA genome sequence. Virology,2001,286(1):7-22.
[95]Lo C.F, Ho C.H, Peng S.E, et al. White spot syndrome baculovirus(WSBV)detected in cultured and captured shrimp, crabs and other arthropods. Diseases of Aquatic Organism,1996,27:215-225.
[96]何建国,周化民,姚伯等.白斑病杆状病毒的感染途径和宿主种类.中山大学学报,1999,14(4)：358-361.
[97]van D.B. Botterblom M.H.A, Huisman E.A. Preliminary study on haemoeyte response to White Spot Syndrome virus infection in black tiger shrim Penaeus monodom. Diseases of Aquatic Organism,2002, 51:149-155.
[98]朱建中,夏晓勤,陆承平等.斑点杂交检测对虾白斑综合征病毒青岛株在鳌虾体内的动态分布.中国病毒学,2001,16(1)：92-95.
[99]张吕平,胡超群,任春华.对虾白斑杆状病毒(WSBV)对斑节对虾的实验感染与病理学研究.热带海洋,2000,19(2)：69-73.
[100]Yoganandhan K, Narayanan R.B, Sahul H.A. Larvae and early post-larvae of Penaeus monodon (Fabrieius) experimentally infected with white spot syndrome virus(WSSV) show no signifieant mortality. Journal of fish diseases,2003,26(7):385 — 391.
[101]刘广斌.白斑综合症病毒(wssv)在养殖水体中传播与预防的研究.中国海洋大学硕士学位论文,2004：6.
[102]Soto M.A, Lotz J.M, Epidemiological parameters of white spot syndrome virus infecion in Litopenaeus vannamei and L.setiferus. Journal of Invertebrate Pathology,2001,78(1):9-15.
[103]Maeda M, Kasornchandra J, Itami T, et al. Effect of various treatments on white spot syndr-ome virus(WSSV)from Penaeus japonicus(Japan)and Pmonodon(Thailand).Fish pathology,1998,33(4)：381-387.
[104]战文斌,邢靖,王远红,铃木信一,福田颖穗.聚合酶链反应应(PCR)检测养殖对虾的白斑症病毒(WSSV)感染.中国水产科学,2000,7(1)：51-54.
[105]宋晓玲,王崇明.皮下及造血组织坏死杆状病毒对中国对虾亲虾的人工感染.水产学报,1996,20(4)：374—378.
[106]雷质文,黄捷,史成银.白斑综合症病毒(WSSV)的宿主调查.海洋与湖沼,2002,33(3)：250-258.
[107]江世贵,翁少萍,吕玲等.对感染白斑综合症病毒的亲虾子代的跟踪检测.中山大学学报(自然科学版),2003a,42(1)：66-69.
[108]崔俊,董蜻,燕惠卿.放流增殖对虾病毒检测报告.水产科学,2002,21(4)；40-41.
[109]何建国,莫福.对虾白斑综合症病毒暴发流行与传播途径、气候和水体理化因子的关系及其控制措施.中国水产,1999,7：34-41.
[110]刘萍,孔杰,李健等.暴发性流行病病原对中国对虾仔虾的人工感染实验研究.海洋科学,1998,(1)：1-3.
[111]Vidal O.M,Granja C.B,Aranguren F, et al. A profound effect of hyperthermia on survival of Litopenaeus vannamei juveniles infected with white spot syndrome virus. Journal of world Aquaculture society,2001(32):364-372.
[112]Liu B, Yu Z M, Song X X, et al. The effect of acute salinity change on white spot syndrome (WSSV) outbreaks in Fenneropenaeus chinensis. Aquaculture,2006,253(1-4):163-170.
[113]李才文,管越强,俞志明.盐度变化对日本对虾暴发白斑综合症病毒病的影响.海洋环境科学2002,21(4)：6-9.
[114]王克行,马甡,李晓甫.试论对虾白斑病暴发的环境因子及防病措施.中国水产,1998,12：34-35.
[115]马建新,刘爱英,宋爱芹.对虾病毒病与化学需氧量的相关关系研究.海洋科学,2002,26(3)：68-71.
[116]李志华,王军霞,谢松.环境因子在虾类养殖中的作用分析.水利渔业,2004,24(5)：1-4.
[117]潘鲁青,姜令绪.盐度、pH突变对2种养殖对虾免疫力的影响.青岛海洋大学学报,2002,32(6)：903-910.
[118]姜令绪,潘鲁青,肖国强.氨氮对凡纳对虾免疫指示的影响.中国水产科学,2004,11(6)：537-541.
[119]吕玉华,金征宇,徐学明.饲料中添加富含虾青素的法夫酵母对罗氏沼虾的体色及生长状况的影响.水产养殖1999,(4)：107-112.
[120]裴素蕊.饲料中添加虾青素对凡纳滨对虾的影响和作用机理.河北大学硕士学位论文,2009：19.
[121]杨景山.医学细胞化学与细胞生物技术.北京医科大学中国协和医科大学联合出版社,1992,6：21-31.
[122]Wang W.N, Wang A.L, Zhang Y.J. Effect of dietary higher level of selenium and nitrite con-centration on the cellular defense response of Penaeus vannamei. Aquaculture,2006,256:55 8-563.
[123]欧阳琴,陈兴才,黄亚治.雨生红球藻虾青素提取工艺条件研究.福建轻纺2003,175(12)：9-14.
[124]范秀容,沈萍.微生物学实验.高等教育出版社,1980,112-113.
[125]王雷,李光友,毛远新等.郝莎等.口服免疫型药物对养殖中国对虾病害防治作用的研究.海洋与湖沼,1994,25(5)：486-491.
[126]刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究.海洋与湖沼,1998,29(2)：113-117.
[127]吕玲,何建国,谢数涛等.白斑综合杆状病毒(WSBV)PCR方法的改进及应用.热带海洋,2000,19(2)： 91-98.
[128]江晓路,刘树青.真菌多糖对中国对虾血清及淋巴细胞免疫和免疫活性的影响.动物学研究,1999,20(1)：41-45.
[129]HuangZeng-Tai.The effeets of different immunostimulants on the non-speeific inunune response and physiological factors of white shrimp(Litopenaeus vannamei)台湾海洋大学硕士学位论文,2005：8.
[130]Cheng W, Liu C.H, Yeh S.T, et al.The inimune stimulatory effeet of sodium alginate on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus.Fish and Shellfish Immunology,2004,17:41-51.
[131]汪小锋.中国对虾(Penaeus chinensis)酚氧化酶的生物化学性质及几种免疫促进剂对中国对虾酚氧化酶和血细胞的影响.中国海洋大学硕士学位论文.2002,5.
[132]Sakai M, Taniguehi K, Mamoto K, et, al. Immunostimulant effeets of nueleotide isolated from yeast RNA on carp, Cyprinus carpio. Journal of fish diseases,2001,24(8):433-438.
[133]Amar E.C, Kiron V, Satoh S, et al. Influence of various dietary synthetic carotenoids on bio-defence mechanisms in rainbowtrout,Oncorhynchusmykiss(Walbaum). Aquaculture Research,20 01(2):62-173.
[134]Chang C.F, Su M.S, Chen H.Y, et al.Effect of dietary beta-1,3-glucan on resistance to white spot syndrome virus(WSSV) in postlarval and juvenile Penaeus monodon. Disease of Aquatic Organisme,1999,36:163-168.
[135]谭北平,周歧存,郑石轩等.β-1,3／1,6-葡聚糖汁及对凡纳对虾生长及免疫力的影响.高技术通 讯,2004,14(5)：73-77.
[136]Sitdhi B, Mali B. Effects of peptidoglycan(PG) on growth,survival, Immune response and tolerance to stress in black tiger shrimp,Penaeus mondon. In:Diseases in Asian Aquaculture 11.1998.469-477.
[137]Takahasi Y, Uehara K, Watanabe R. Efficacy of oral administration of fucoidan, a sulfated polysaccharide, in controlling white spot syndrome in Kumura Shrimp in Japan. Advances in Shrimp Biotechnology,1998.171-173.
[138]Kumlu M, Fletcher D.J, Fisher C.M. Larval pigmentation, survival and growth of Penaeus indicus fed the nematode Panagrellus redivivus enriched with astaxanthin and various lipids. Aquaculture Nurtriton,1998,4:193-200.
[139]Pan C.H, Chien Y.H, Hunter B. Alterations of antioxidant capacity and hepatopancreatic enzymes in Penaeus monodon (Fabricius) juveniles fed diets supplemented with astaxanthin and exposed to Vibrio damsela challenge. Journal of Fisheries Society of Taiwan(JournalSeek),2003,30:279-290.
[140]林金明.活性氧的化学发光测定法.环境科学学报,2003,23：230-238.
[141]燕国梁.活性氧胁迫下Bacillus sp.F26以过氧化氢酶合成为特征的应激响应.江南大学博士学位论文.2006,4-5.
[142]滕旭,武文琦,周显青.维生素C多聚磷酸酚对小鼠肝脏抗氧化物酶基因转录的影响.动物学报,2006,52(6)：1107-1112.
[143]国家海洋局.中华人民共和国国家标准海水监测规范.北京：中国标准出版社,2007：GB／T12763.4-2007.
[144]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法.北京：中国环境科学出版社,2002：12.
[2]李红霞.CpG寡脱氧核苷酸对中华绒鳌蟹免疫功能调节作用的研究.苏州大学硕士学位论文,2007.11.
[3]叶燕玲,陈宽智.中国对虾血细胞超微结构、分类及计数.青岛海洋大学学报,1993,23(2)：35-42.
[4]Soderhall K, Smith V. J, Johansson M. W. Exocytosis and uptake of bacteria by isolated haemocyte populations of two crustaceans:evidence for cellular co-operation in the defence reactions of arthropods. Cell and Tissue Research,1986,245:43-49.
[5]Jenny R. Gilles L.M. State of art of immunological tools and health control of penaeid shrimp. Aquaculture,2000:109-119.
[6]Bayne C.J. Phagocytosis and non-self recognition in invertebrates. Phagocyteosis appears to be an ancient line of defence. BioScience,1990,40:723-731.
[7]徐海圣,徐步进.甲壳动物细胞及体液免疫机理的研究进展.大连水产学报,2001,16(1)：41-44.
[8]刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究.海洋与湖沼,1998,29(2)：113-117.
[9]刘树青,江晓路,牟海津.免疫多糖对日本对虾血清酶活性的研究.中国水产科学,1999,6(3)：11 1-113.
[10]王雷,李光友,毛远兴.中国对虾血淋巴中抗菌、溶菌活力与酚氧化酶活力的测定及其特性研究.海洋与湖沼,1995,26(2)：179-18.
[11]Alexandra A. Response of penaeid shrimp to exposure to Vibrio species. Fish & Shellfish Immunology, 1991, 1(1):59-70.
[12]Destoumieux D, Bulet P, Loew D, et.al. A new family of antimicrobial peptides isolated from the Penaeus vannamei(Decapode). The Journal of Biology Chemistry,1997,272:28398-28406.
[13]庄承纪.壳多糖对罗氏沼虾、斑节对虾苗生长和抗菌防病作用研究.湛江海洋大学学报,1998,1 8(3)：29～34.
[14]曹丹,周洪琪.壳聚糖对异育银鲫的生长、蛋白质合成及肌肉营养成分的影响.淡水渔业,2004,34(1)：6-9.
[15]刘兴国.低分子壳聚糖对罗非鱼肝组织抗氧化能力和肝脂含量影响研究.海洋渔业,2004,26(4)：291-294.
[16]胡品虎.稀土甲壳素在河蟹养殖中的应用.水产养殖,1999,(5)：13～14.
[17]江晓路,刘树青,牟海律等.真菌多糖对中国明对虾血清及淋巴细胞免疫影响.动物学研究,1999,20(1)：41～45.
[18]许国焕.两种多聚糖对彭泽鲫生长影响及免疫促进作用的初步研究.水利渔业,2002,22(4)：49～51.
[19]牟海津,江晓路,刘树青,等.免疫多糖对栉孔扇贝酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶活性的影响.青岛海洋大学学报,1999,25(3)：463～468.
[20]刘树青,江晓路,牟海津,等.免疫多糖对日本对虾血清酶活性的影响.中国水产科学,1999,6(3)：107-109.
[21]刘岩,江晓路,吕青,等.聚甘露糖醛酸对虾免疫相关酶活胜和溶菌溶血活性的影响.水产学报,2000,24(6)：549～553.
[22]刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究.海洋与湖沼,1998,29(2)：113～118.
[23]Chang C.F, Su M.S, Chen H.Y. Vibriosis resistance and wound healing enhancement of Penaeus monodon by beta-1,3-glucan from Schizophyllum commune and polyphosphorylated 1-ascorbic acid. Journal of Taiwan fisheries Research.1996 4:43～54.
[24]谭北平,周歧存,郑石轩等.β-1,3／1,6-葡聚糖制剂对凡纳对虾生长及免疫力的影响.高技术通讯2004.5：73-78.
[25]Itami T, Asano M, Tokushige K, et.al. Enhancement of disease resistance of kuruma shrimp, Peneus japonicus, after oral adminitraion of peptidoglycan derived from Bifibacterium thermophilum. Aquaculture,1998,164:277-288.
[26]孟凡伦,张玉臻.甲壳动物中的酚氧化酶原激活系统研究评价.海洋与湖沼,1999,30(1)：110-115.
[27]王秀华,宋晓玲.肽聚糖制剂对南美白对虾体液免疫因子的影响.中国水产科学,2004,11(1)：26-30.
[28]黎中宝编.饲料添加剂.厦门：厦门大学出版社.2004.
[29]崔青曼,张耀红.中草药、多糖复方添加剂提高河蟹机体免疫力的研究.水利渔业,2001,21(4)：40～41.
[30]罗日祥.中药制剂对中国对虾免疫活性物质的诱导作用.海洋与湖沼,1997,28(6)：573～576.
[31]杜爱芳.复方大蒜油添加剂对中国对虾免疫机能的增强作用.浙江大学学报,1997,23(3)：317～320.
[32]Sirirat R, Sombat R, Somkiat P, et al. Immunity enhancement in black tiger shrimp by a probiont bacterium(Bacillus Sll). Aquaculture,2000,191(4,5):271-288.
[33]蔡刚,王杉霖,李文权等.天然虾青素在水产养殖业中的应用研究进展.台湾海峡,2003.22(3)：377-385.
[34]杨艳.虾青素抗氧化活性机制研究进展.国外医学卫生学分册.2008,35(4)：23 1-233.
[35]张明祥,赵建国.国内外虾青素的研究进展.粮食与饲料工业,2002(1)：26-28.
[36]Lee S H, David B.M. Effects, quenching mechanisms and kinetics of carotenoids in chloroph-yll sensitized photooxidafion of soybean oil. Journal of Agricultural and Food Chemistry,1990, 38:1630-1634.
[37]Chien Y.H., Pan C.H., Hunter B. The resistance to physical stresses by Penaeus monodon juveniles fed diets supplemented with astaxanthin. Aquaculture,2003,216(1-4):177-191.
[38]Pan C.H., Chien Y.H., Hunter B.The resistance to ammonia stress of Penaeus monodon Fabricius juvenile fed diets supplemented with astaxanthin. Journal of Experimental Marine Biology and Ecology,2003,297:107-118.
[39]Wang Y J, Chien Y H, Pan C H. Effects of dietary supplementation of carotenoids on survival, growth, pigmentation, and antioxidant capacity of characins, Hyphessobrycon callistus. Aquaculture,2006, 261:641-648.
[40]Yamada S. Pigmentation of prawn (Penaeus japonicus) with carotenoids:effect of dietary astaxanthin,-carotene and canthaxanthin on pigmentation. Aquaculture,1990,87(3-4):323-330.
[41]Menasveta P, Worawattanamateekul W, Latscha Y, et al. Correction of tiger prawn(Penaeus monodon Fabricus) coloration by astaxanthin. Aquacultural Engineering,1993,12:(4) 203-213.
[42]Merchie, Lavens P. Effect of vitamin C and astaxanthin on stress and disease resistance of postlarval tiger shrimp penaeus monodon (Fabricius). Aquaculture Research,1998,29:579-585.
[43]Chien YH. Shiau W. C. The effects of dietary supplementation of algae and synthetic astaxan thin on body astaxanthin,survival,growth, and low dissolved oxygen stress resistance of kuruma prawn, Marsupenaeus japonicus Bate. Journal of Experimental Marine Biology and Ecology, 2005,318:201-211.
[44]Pan,C.H,Chien YH, Cheng J.H. Effect of light regime, algae in the water, and dietary astaxanthin on pigmentation, growth, and survival of black tiger prawn Penaeus monodon post-larvae. Zoological Studies,2001,40(4):371-382.
[45]潘志弘.影响草虾类胡萝卜素分布因素及虾红素在草虾生物功能之研究.国立台湾海洋大学,2001.
[46]Lutsenko E.A, Carcamo J.M, Golde D.M. Vitamin C prevents DNA mutation induced by oxidative stress.The Journal of Biological Chemistry.2002,277(19):16895-16899.
[47]卫涛涛.生物大分子的氧化损伤与疾病.怀化医专学报,2006,5(1)：81-84.
[48]Schneider M, Diemer K, Engelhart K,et al. Protective effects of vitamins C and E on the number of micronuclei in lymphocytes in smokers and their role in ascorbate free radical formation in plasma. Free Radical Research,2001,34 (3):209-219.
[49]Cetinkaya O, Silig Y, Cetinkaya S,et al. The effects of Rumex patientia extract on rat liver and erythrocyte antioxidant enzyme system. Pharmazie,2002,57(7):487-488.
[50]查锡良.生物化学.北京：人民卫生出版社,2001,168-172.
[51]Fridovich I. Superoxide dismutases:studies of structure and mechanism. Advance Experiment Medicine Biology.1976,74:530-539.
[52]Geret F, Serafim A, Barreira L, et al. Response of antioxidant systems to copper in the gills of the clam Ruditapes decussatus. Marine Environment Research,2002,54(3-5):413-417.
[53]Khachik F, Carvalho L, Bernstein P.S, et al. Chemistry, distribution, and metabolism of tomato carotenoids and their impact on human health. Experimental Biology and Medicine,2002,227 (10): 845-851.
[54]Cheng W, Tung Y H, Chiou T T,et al. Cloning and characterisation of mitochondrial manganese superoxide dismutase (mtMnSOD) from the giant freshwater prawn Macrobrachium rosenbergii.Fish & Shellfish Immunology,2006,21(4):453-466.
[55]Cheng W, Tung Y H, Liu C H, et al. Molecular cloning and characterisation of copper/zinc superoxide dismutase (Cu/ZnSOD) from the giant freshwater prawn Macrobrachium rosenbergii. Fish & Shellfish Immunol,2006,21(1):102-112.
[56]Gomez A.G, Barillas C.V, Peregrino A.B, et al. The cytosolic manganese superoxide dismutase from the shrimp Litopenaeus vannamei:Molecular cloning and expression. Developmental & Comparative Immunology,2006,30 (10):893-900.
[57]Ken C.F, Weng D.F, Duan K.J, et al. Characterization of copper/zinc-superoxide dismutase from Pagrus major cDNA and enzyme stability. Journal of Agricultural Food Chemistry,2002,50(4): 784-789.
[58]Pannunzio T.M, Storey K.B. Antioxidant defenses and lipid peroxidation during anoxia stress and aerobic recovery in the marine gastropod Littorina littorea. Journal of Experimental Marine Biology and Ecology,1998,221(2):277-292.
[59]Willmore W.G, Storey K.B. Antioxidant systems and anoxia tolerance in a freshwater turtle Trachemys scripta elegans. Molecular and Cellular Biochemistry,170(1-2):177-185.
[60]Chetty A.N, Indira K. Protection against free radical toxicity in mantle and foot of a fresh water bivalve exposed to Ambient ammonia. Proceeding of the National Academy of Sciences of India,1994, 64(1):35-40.
[61]白洁,唐学玺.久效磷对中国对虾体内SOD活力影响的初步研究.海洋通报,1998,17(41-45).
[62]Zikic V, Ognjanovic B.I,Pavlovic S.Z, et al. Activities of superoxide dismutase and catalse in erythrocytes and transminasea in the plasma of carps(Cyprinus carpio L.)exposed to cadium Physiology Research,1997,46 (5):391-396.
[63]Doyotte A, Cossu C, Jacquin M C, et al. Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve Unio tumidus. Aquatic Toxicology,1997,39(2):93-110.
[64]Brouwer M B. Biochemical defense mechanisms against copper include oxidative damage in the blue crab,Callinects sapidus. Archives of Biochemistry Biophysics 1998(2):257-264.
[65]Parihar M.S, Javeri T,Hemnani T, et al. Responses of superoxide dimutase,glutathione peroxidase and reduced glutathione antioxidant defense in gills of the fresh water catfish (Heteropneusres fossilis) to short term elevated temperature. Journal of Thermal Biology,1997,22 (2):151-156.
[66]Wilhelm F.D. Fish antioxidant defenses comparative approach. Brazilian Journal of Medical Biological Research,1996,29(12):1735-1742.
[67]牟海津,江晓路,刘树清.免疫多糖对栉孔扇贝酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶活性的影响.青岛海洋大学学报,1999,29(3)：462-468.
[68]刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究.海洋与湖沼,1998,29(2)：113-118.
[69]孟繁伦,马桂容,孔建.养殖中国对虾益生素制品的研究.山东大学学报,1999,33(1)：101-105.
[70]Boutet I, Tanguy A, Moraga D. Response of the Pacific oyster Crassostrea gigas to hydrocarbon contamination under experimental conditions.Gene,2004,329:147-157.
[71]Labreuche Y, Lambert C, Soudant P, et al. Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32. Microbes and Infection,2006,8(12-13):2715-2724.
[72]Goodall C.P, Bender R.C, Broderick E.J, et al. Constitutive differences in Cu/Zn superoxide d-ismutase mRNA levels and activity in hemocytes of Biomphalaria glabrata(Mollusca)that are either susceptible or resistant to Schistosoma mansoni(Trematoda).Molecular and Biochemical P-arasitology,2004,137(2):321-328.
[73]Zelck U.E, Janje B, Schneider O. Superoxide dismutase expression and H2O2 production by hemocytes of the trematode intermediate host Lymnaea stagnalis(Gastropoda).Developmental & Comparative Immunology,2005,29(4):305-314.
[74]Yuan H.T, Bingle C.D, Kelly F.J. Differential patterns of antioxidant enzyme mRNA expression in guinea pig lung and liver during development. Biochimica BiophysicaActa,1996,1305 (3):163～171.
[75]Gomez-Anduro GA, Barillas-Mury C.V, Peregrino-Urate A.B, et al. The cytosolic manganese superoxide dismutase from the shrimpLitopenaeus vannamei:Molecular cloning and expression. Developmental & Comparative Immunology,2006,30(10):893～900.
[76]Tavares-Sanchez O.L,Gomez-Anduro GA, Felipe-Ortega X,et al.The Catalase from the white shrimp Penaeus(Litopenaeus vannamei):molecular cloning and protein detection. Comparative Biochemistry and Physiology,2004,138(4):331～337.
[77]瞿建宏,胡庚东,吴伟.2006.苯酚胁迫下罗非鱼组织中过氧化氢酶与谷胱甘肽-S-转移酶的动态变化生态环境(4)：687-690.
[78]黄长江,董巧.2006.苯并芘对罗非鱼肝脏抗氧化防御系统的影响.汕头大学学报(自然科学版)(4)：5 1-56.
[79]Dandapat J, Chainy G B, Janardhana Rao K. Dietary vitamin E modulates antioxidant defence system in giant freshwater prawn, Macrobrachium rosenbergii.Comparative Biochemistry and Physiology,2000,127 (1):101-115.
[80]Cheung C.C, Zheng GJ, Li A.M, et al. Relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquatic Toxicology,2001,52(3-4):189-203.
[81]Krishnakumar P.K, Casillas E, Snider R.G, et al.Environmental contaminants and the prevalence of hemic neoplasia(leukemia) in the common mussel(Mytilus edulis complex)from Puget Sound, Washington, U.S.A. Journal of Invertebrate Pathology,1999,73(2):135-46.
[82]Gerhard GS, Kauffman E.J, Grundy M.A. Molecular cloning and sequence analysis of the Danio rerio catalase gene.Comparative Biochemistry and Physiology,2000,127(4):447～457.
[83]孙翰昌,丁诗华.Cu2-中华倒刺鲤抗氧化功能的毒理效应.农业环境科学学报,2006,25(1)：69-72.
[84]Santovito G, Piccinni E, Cassini A, et al. Antioxidant responses of the mediterranean mussel, Mytilus galloprovincialis,to environmental variability of dissolved oxygen. Comparative Biochemistry and Physiology,2005,140(3-4):321-329.
[85]Company R, Serafim A, Bebianno M.J, et al. Effect of cadmium,copper and mercury on antioxidant enzyme activities and lipid peroxidation in the gills of the hydrothermal vent mussel Bathymodiolus azoricus. Marine Environmental Research,2004,58(2-5):377-381.
[86]Fukuhara R, Kageyama T. Tissue distribution, molecular cloning, and gene expression of cytosolic glutathione peroxidase in Japanese monkey. Zoological Science,2003,20(7):861-868.
[87]Singh A.K, Shichi H. A novel glutathione peroxidase in bovine eye Sequence analysis, mRNA level, and translation. The Journal of Biochemistry,1998,273(40):26171-26178.
[88]Knopp E.A, Arndt T.L, Eng K, et al. Murine phospholipid hydroperoxide glutathione peroxidase:cDNA sequence,tissue expression,and mapping. Mammal Genome,1999,10(6):601-605.
[89]倪多娇.栉孔扇贝参与氧化反应基因的克隆与表达分析.中国科学院海洋研究所博士学位论文,2007：87.
[90]许雅香,许梓荣,朱玉芳.对虾白斑综合征病毒分子生物学研究进展Marine Sciences,2004,28 (12):63-65.
[91]吴信忠.中国海洋病害主流研究的进展.太平洋学报,2005,10：49-59.
[92]孙成波,何建国,黎子兰等.凡纳滨对虾和斑节对虾对WSSV敏感性的比较.湛江海洋大学学报,2006,26(3)：17-20.
[93]张立人,张建红,陈棣华等.东方对虾杆状病毒在宿主细胞内装配.电子显微镜学报,1994,13(5)354.
[94]Van H.M,Wittteveldt J, Peters S, et al. The white spot syndrome virus DNA genome sequence. Virology,2001,286(1):7-22.
[95]Lo C.F, Ho C.H, Peng S.E, et al. White spot syndrome baculovirus(WSBV)detected in cultured and captured shrimp, crabs and other arthropods. Diseases of Aquatic Organism,1996,27:215-225.
[96]何建国,周化民,姚伯等.白斑病杆状病毒的感染途径和宿主种类.中山大学学报,1999,14(4)：358-361.
[97]van D.B. Botterblom M.H.A, Huisman E.A. Preliminary study on haemoeyte response to White Spot Syndrome virus infection in black tiger shrim Penaeus monodom. Diseases of Aquatic Organism,2002, 51:149-155.
[98]朱建中,夏晓勤,陆承平等.斑点杂交检测对虾白斑综合征病毒青岛株在鳌虾体内的动态分布.中国病毒学,2001,16(1)：92-95.
[99]张吕平,胡超群,任春华.对虾白斑杆状病毒(WSBV)对斑节对虾的实验感染与病理学研究.热带海洋,2000,19(2)：69-73.
[100]Yoganandhan K, Narayanan R.B, Sahul H.A. Larvae and early post-larvae of Penaeus monodon (Fabrieius) experimentally infected with white spot syndrome virus(WSSV) show no signifieant mortality. Journal of fish diseases,2003,26(7):385 — 391.
[101]刘广斌.白斑综合症病毒(wssv)在养殖水体中传播与预防的研究.中国海洋大学硕士学位论文,2004：6.
[102]Soto M.A, Lotz J.M, Epidemiological parameters of white spot syndrome virus infecion in Litopenaeus vannamei and L.setiferus. Journal of Invertebrate Pathology,2001,78(1):9-15.
[103]Maeda M, Kasornchandra J, Itami T, et al. Effect of various treatments on white spot syndr-ome virus(WSSV)from Penaeus japonicus(Japan)and Pmonodon(Thailand).Fish pathology,1998,33(4)：381-387.
[104]战文斌,邢靖,王远红,铃木信一,福田颖穗.聚合酶链反应应(PCR)检测养殖对虾的白斑症病毒(WSSV)感染.中国水产科学,2000,7(1)：51-54.
[105]宋晓玲,王崇明.皮下及造血组织坏死杆状病毒对中国对虾亲虾的人工感染.水产学报,1996,20(4)：374—378.
[106]雷质文,黄捷,史成银.白斑综合症病毒(WSSV)的宿主调查.海洋与湖沼,2002,33(3)：250-258.
[107]江世贵,翁少萍,吕玲等.对感染白斑综合症病毒的亲虾子代的跟踪检测.中山大学学报(自然科学版),2003a,42(1)：66-69.
[108]崔俊,董蜻,燕惠卿.放流增殖对虾病毒检测报告.水产科学,2002,21(4)；40-41.
[109]何建国,莫福.对虾白斑综合症病毒暴发流行与传播途径、气候和水体理化因子的关系及其控制措施.中国水产,1999,7：34-41.
[110]刘萍,孔杰,李健等.暴发性流行病病原对中国对虾仔虾的人工感染实验研究.海洋科学,1998,(1)：1-3.
[111]Vidal O.M,Granja C.B,Aranguren F, et al. A profound effect of hyperthermia on survival of Litopenaeus vannamei juveniles infected with white spot syndrome virus. Journal of world Aquaculture society,2001(32):364-372.
[112]Liu B, Yu Z M, Song X X, et al. The effect of acute salinity change on white spot syndrome (WSSV) outbreaks in Fenneropenaeus chinensis. Aquaculture,2006,253(1-4):163-170.
[113]李才文,管越强,俞志明.盐度变化对日本对虾暴发白斑综合症病毒病的影响.海洋环境科学2002,21(4)：6-9.
[114]王克行,马甡,李晓甫.试论对虾白斑病暴发的环境因子及防病措施.中国水产,1998,12：34-35.
[115]马建新,刘爱英,宋爱芹.对虾病毒病与化学需氧量的相关关系研究.海洋科学,2002,26(3)：68-71.
[116]李志华,王军霞,谢松.环境因子在虾类养殖中的作用分析.水利渔业,2004,24(5)：1-4.
[117]潘鲁青,姜令绪.盐度、pH突变对2种养殖对虾免疫力的影响.青岛海洋大学学报,2002,32(6)：903-910.
[118]姜令绪,潘鲁青,肖国强.氨氮对凡纳对虾免疫指示的影响.中国水产科学,2004,11(6)：537-541.
[119]吕玉华,金征宇,徐学明.饲料中添加富含虾青素的法夫酵母对罗氏沼虾的体色及生长状况的影响.水产养殖1999,(4)：107-112.
[120]裴素蕊.饲料中添加虾青素对凡纳滨对虾的影响和作用机理.河北大学硕士学位论文,2009：19.
[121]杨景山.医学细胞化学与细胞生物技术.北京医科大学中国协和医科大学联合出版社,1992,6：21-31.
[122]Wang W.N, Wang A.L, Zhang Y.J. Effect of dietary higher level of selenium and nitrite con-centration on the cellular defense response of Penaeus vannamei. Aquaculture,2006,256:55 8-563.
[123]欧阳琴,陈兴才,黄亚治.雨生红球藻虾青素提取工艺条件研究.福建轻纺2003,175(12)：9-14.
[124]范秀容,沈萍.微生物学实验.高等教育出版社,1980,112-113.
[125]王雷,李光友,毛远新等.郝莎等.口服免疫型药物对养殖中国对虾病害防治作用的研究.海洋与湖沼,1994,25(5)：486-491.
[126]刘恒,李光友.免疫多糖对养殖南美白对虾作用的研究.海洋与湖沼,1998,29(2)：113-117.
[127]吕玲,何建国,谢数涛等.白斑综合杆状病毒(WSBV)PCR方法的改进及应用.热带海洋,2000,19(2)： 91-98.
[128]江晓路,刘树青.真菌多糖对中国对虾血清及淋巴细胞免疫和免疫活性的影响.动物学研究,1999,20(1)：41-45.
[129]HuangZeng-Tai.The effeets of different immunostimulants on the non-speeific inunune response and physiological factors of white shrimp(Litopenaeus vannamei)台湾海洋大学硕士学位论文,2005：8.
[130]Cheng W, Liu C.H, Yeh S.T, et al.The inimune stimulatory effeet of sodium alginate on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus.Fish and Shellfish Immunology,2004,17:41-51.
[131]汪小锋.中国对虾(Penaeus chinensis)酚氧化酶的生物化学性质及几种免疫促进剂对中国对虾酚氧化酶和血细胞的影响.中国海洋大学硕士学位论文.2002,5.
[132]Sakai M, Taniguehi K, Mamoto K, et, al. Immunostimulant effeets of nueleotide isolated from yeast RNA on carp, Cyprinus carpio. Journal of fish diseases,2001,24(8):433-438.
[133]Amar E.C, Kiron V, Satoh S, et al. Influence of various dietary synthetic carotenoids on bio-defence mechanisms in rainbowtrout,Oncorhynchusmykiss(Walbaum). Aquaculture Research,20 01(2):62-173.
[134]Chang C.F, Su M.S, Chen H.Y, et al.Effect of dietary beta-1,3-glucan on resistance to white spot syndrome virus(WSSV) in postlarval and juvenile Penaeus monodon. Disease of Aquatic Organisme,1999,36:163-168.
[135]谭北平,周歧存,郑石轩等.β-1,3／1,6-葡聚糖汁及对凡纳对虾生长及免疫力的影响.高技术通 讯,2004,14(5)：73-77.
[136]Sitdhi B, Mali B. Effects of peptidoglycan(PG) on growth,survival, Immune response and tolerance to stress in black tiger shrimp,Penaeus mondon. In:Diseases in Asian Aquaculture 11.1998.469-477.
[137]Takahasi Y, Uehara K, Watanabe R. Efficacy of oral administration of fucoidan, a sulfated polysaccharide, in controlling white spot syndrome in Kumura Shrimp in Japan. Advances in Shrimp Biotechnology,1998.171-173.
[138]Kumlu M, Fletcher D.J, Fisher C.M. Larval pigmentation, survival and growth of Penaeus indicus fed the nematode Panagrellus redivivus enriched with astaxanthin and various lipids. Aquaculture Nurtriton,1998,4:193-200.
[139]Pan C.H, Chien Y.H, Hunter B. Alterations of antioxidant capacity and hepatopancreatic enzymes in Penaeus monodon (Fabricius) juveniles fed diets supplemented with astaxanthin and exposed to Vibrio damsela challenge. Journal of Fisheries Society of Taiwan(JournalSeek),2003,30:279-290.
[140]林金明.活性氧的化学发光测定法.环境科学学报,2003,23：230-238.
[141]燕国梁.活性氧胁迫下Bacillus sp.F26以过氧化氢酶合成为特征的应激响应.江南大学博士学位论文.2006,4-5.
[142]滕旭,武文琦,周显青.维生素C多聚磷酸酚对小鼠肝脏抗氧化物酶基因转录的影响.动物学报,2006,52(6)：1107-1112.
[143]国家海洋局.中华人民共和国国家标准海水监测规范.北京：中国标准出版社,2007：GB／T12763.4-2007.
[144]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法.北京：中国环境科学出版社,2002：12.