海洋微藻活性微量元素积累的研究
摘要
研究以普通小球藻为材料,设定8个添加硒[Ⅳ]浓度处理,6个添加Cr3+浓度处理,每组浓度水平设两个平行样,测定了不同硒[Ⅳ]浓度和Cr3+浓度下小球藻的生物量(细胞密度)、小球藻对硒[Ⅳ]和Cr3+的富集量。结果表明,当硒[Ⅳ]浓度大于1mg/L时,小球藻的生长受到不同程度的抑制,当硒[Ⅳ]浓度大于12mg/L时,可导致藻体的死亡沉积。小球藻在硒[Ⅳ]浓度为1mg/L～8mg/L的范围内,随着硒[Ⅳ]浓度的增加,小球藻的富集量随之升高,从小球藻富集硒生产的经济效益、社会效益和疗效综合考虑,我们认为培养液中适宜的添加硒浓度为8mg/L,在此浓度下,小球藻的生物量较高,为0.25mg/L,富集量也较高,为83.66ug/g；当添加Cr3+浓度大于32mg/L时,小球藻的生长受到不同程度的抑制,当添加Cr3+浓度大于300mg/L时,可导致藻体的死亡沉积。富Cr3+培养小球藻的培养基中会产生大量的沉淀,沉淀率为99.74%。小球藻在添加Cr3+浓度为32mg/L～100mg/L的范围内(实际Cr3+浓度为0.08758mg/L～0.5719mg/L),随着Cr3+浓度的增加,小球藻的富集量随之升高,从小球藻富集Cr3+生产的经济效益、社会效益和疗效综合考虑,我们认为培养液中适宜的添加Cr3+浓度为100mg/L(实际Cr3+浓度为0.5719mg/L),在此浓度下,小球藻的生物量较高,为0.26 mg／L,富集量也较高,为9974.968 ug／g。
研究目的在于采用生物富集技术研制出富含活性元素Cr3+和Se[Ⅳ]的高附加值微藻,为大规模养殖和工业化生产提供理论基础。
In this paper, we use the Chlorella pyrenoidosa as the stuff of study, set 8 Se[Ⅳ] concentration and 6 Cr3+ concentration, set two parallel sample in this two group, then testing the biomass and bioaccumulation in Chlorella pyrenoidosa which is added by different Se[Ⅳ] concentration and Cr3+ concentration. As the result, it will have inhibition effects when Se[Ⅳ] concentration exceed 1mg/L, when Se[Ⅳ] concentration is up to 12 mg/L, the growth of algae cell nearly stops. Se[Ⅳ] bioaccumulation in Chlorella pyrenoidosa will rise up with the increase of Se[Ⅳ] concentration when the Se[Ⅳ] concentration is 1 mg/L-8 mg/L. Consideration of the economic effects、social effects and therapeutic effects of the Se[Ⅳ] bioaccumulation in Chlorella pyrenoidosa, I think the best Se[Ⅳ] concentration in culture solution is 8 mg/L, in this concentration, the biomass of Chlorella pyrenoidosa is 0.25 mg/L, and the bioaccumulation of Chlorella pyrenoidosa is 83.66 ug/g; It will have inhibition effects when Cr3+ concentration exceed 32 mg/L, when Cr3+ concentration is up to 300 mg/L, the growth of algae cell nearly stops. The culture solution of Cr3+ bioaccumulation create a lot of deposit, the rate of the deposition is 99.74%.Cr3+ bioaccumulation in Chlorella pyrenoidosa will rise up with the increase of Cr3+ concentration when the Cr3+ concentration is 32 mg/L-100 mg/L(actually the Cr3+ concentration is 0.08758 mg/L～0.5719 mg/L). Consideration of the economic effects、social effects and therapeutic effects of the Cr3+ bioaccumulation in Chlorella pyrenoidosa, I think the best Cr3+ concentration in culture solution is 100 mg/L(actually the Cr3+ concentration is 0.5719 mg/L), in this concentration, the biomass of Chlorella pyrenoidosa is 0.26 mg/L, and the bioaccumulation of Chlorella pyrenoidosa is 9974.968 ug/g.
This study is intent to adopt bioaccumulation technology to develop high add-ons Chlorella pyrenoidosa which have abundant trace element of Cr3+ and Se[IV],to provide theory foundation of cosmically breed aquatics and industrialization produce.
研究目的在于采用生物富集技术研制出富含活性元素Cr3+和Se[Ⅳ]的高附加值微藻,为大规模养殖和工业化生产提供理论基础。
In this paper, we use the Chlorella pyrenoidosa as the stuff of study, set 8 Se[Ⅳ] concentration and 6 Cr3+ concentration, set two parallel sample in this two group, then testing the biomass and bioaccumulation in Chlorella pyrenoidosa which is added by different Se[Ⅳ] concentration and Cr3+ concentration. As the result, it will have inhibition effects when Se[Ⅳ] concentration exceed 1mg/L, when Se[Ⅳ] concentration is up to 12 mg/L, the growth of algae cell nearly stops. Se[Ⅳ] bioaccumulation in Chlorella pyrenoidosa will rise up with the increase of Se[Ⅳ] concentration when the Se[Ⅳ] concentration is 1 mg/L-8 mg/L. Consideration of the economic effects、social effects and therapeutic effects of the Se[Ⅳ] bioaccumulation in Chlorella pyrenoidosa, I think the best Se[Ⅳ] concentration in culture solution is 8 mg/L, in this concentration, the biomass of Chlorella pyrenoidosa is 0.25 mg/L, and the bioaccumulation of Chlorella pyrenoidosa is 83.66 ug/g; It will have inhibition effects when Cr3+ concentration exceed 32 mg/L, when Cr3+ concentration is up to 300 mg/L, the growth of algae cell nearly stops. The culture solution of Cr3+ bioaccumulation create a lot of deposit, the rate of the deposition is 99.74%.Cr3+ bioaccumulation in Chlorella pyrenoidosa will rise up with the increase of Cr3+ concentration when the Cr3+ concentration is 32 mg/L-100 mg/L(actually the Cr3+ concentration is 0.08758 mg/L～0.5719 mg/L). Consideration of the economic effects、social effects and therapeutic effects of the Cr3+ bioaccumulation in Chlorella pyrenoidosa, I think the best Cr3+ concentration in culture solution is 100 mg/L(actually the Cr3+ concentration is 0.5719 mg/L), in this concentration, the biomass of Chlorella pyrenoidosa is 0.26 mg/L, and the bioaccumulation of Chlorella pyrenoidosa is 9974.968 ug/g.
This study is intent to adopt bioaccumulation technology to develop high add-ons Chlorella pyrenoidosa which have abundant trace element of Cr3+ and Se[IV],to provide theory foundation of cosmically breed aquatics and industrialization produce.
引文
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