化学药剂处理克服库尔勒香梨自交不亲和性及影响香梨品质因素研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
本论文以库尔勒香梨为试验材料,研究了用化学药剂克服香梨的自交不亲和性、植物生长调节剂对香梨果实品质的影响。以及对库尔勒地区香梨和阿克苏地区香梨的果实品质差异性进行了比较,主要研究结果如下:
     1.6种化学药剂处理香梨柱头的结果表明,化学药剂对克服香梨自交不亲和性有一定效果。不同的化学药剂其效果差异,氯化钠+硼酸、鸭梨和砀山梨花粉浸提液处理效果较好,比对照(蒸馏水处理)差异极显著;而GA、IBA、NAA与对照(蒸馏水)处理相比差异不显著。不同化学药剂浓度的处理效果亦不同,以氯化钠5%+硼酸0.3%、鸭梨和砀山梨花粉1:10(M:M)处理效果最好。
     2.盛花期对香梨喷施植物生长调节剂NAA、Eth和BR处理能增加果实的单果重和可溶性固形物含量,都能在一定成都上改善香梨的品质。烯效唑能增加香梨的可溶性固形物的含量,但是处理使香梨的单果重降低,果形虽然均匀但果形小,降低了香梨的产量。GA、GA+6BA和CPPU能增加香梨的单果重但是用GA、GA+6BA和CPPU促进生长的植物生长调节集在盛花期处理香梨,果形指数增大、宿萼果增加、凸顶果增多。影响了果实的外观品质,使果形发育难看,就是果农称之为“公梨”果实,降低了香梨的商品价值。用CCC、IBA处理香梨对香梨的品质无较大影响。
     3.库尔勒地区香梨可溶性固形物含量,可溶性糖,可滴定酸都优于阿克苏地区香梨。库尔勒地区香梨脱萼率也高于阿克苏地区。果形也较阿克苏地区的好看。凸顶果在阿克苏表现突出。经试验可知花期喷洒一定浓度的赤霉素可使香梨花不脱萼或晚脱萼。并行成凸顶果。在阿克苏地区香梨凸顶果明显比库尔勒多。探其原因可能是阿克苏地区土壤中含有的某些矿物质或是有机物质促进香梨根系产生大量的赤霉素,使的香梨的果形发生变化。
     4.90%的粗皮果分布于内膛枝,仅10%分布于外膛,不同部位的粗皮果的各果形比例也不同。粗皮果的硬度大,可滴定酸含量高,可溶性固形物低。
This thesis mostly to research into the use of chemical treatment overcoming self-incomlatibility of the Korla Fragrant pear,the effect on fruit quality by phytohormone And the A Ke Su area pear and the Korla pear’s fruit quality difference were carried out comparison. The results are as follows:
     1.The results showed that six chemicals on the self-incompatibility of Korla Pear,which are treatment of stigma with sodium chloride and Boracic acid,the water solution extracted yali and dangshan pear flowers,Gibberellic,indole butyric acid and naphthalene acetic acid were effective in overcoming self - incompatibility in Korla fragrant Pear, a gametophytic self - incompatibility system. Sodium Chloride and Boracic acid,the water solution extracted yali and dangshan pear flowers were much better than other chemicals and check (diseilled water). The concent ration of the chemicals worked differently in the study,and the effective concent ration was the 5% and 0.3% of the sodium chloride and Boracic acid,1:10(M:M)of the water solution extracted yali and dangshan pear flowers.
     2.The treatments of spraying NAA,Eth and BR in medium stage of flowering that could improve the weight of single fruit and the total soluble soild contents. Uniconazole could improve the total soluble soild contents but reduce the weight of single fruit .All of GA,CPPU ,GA and 6BA could improve the weight of single fruit,but they increase Longitudinal/Sidewise diameter,calyx fruit and stickup end fruit .They decrease worth of the pear. The treatments of spraying CCC and IBA could not improve the pear's fruit quality.
     3. Soluble solids content ,Soluble sugars and Titration acidity of Korla Fragrant pear are better than A Ke Su’s. The highest flower calyx rate is in Korla.There are lots of Stickup end fruit in A Ke Su. The treatments of spraying GA could not shed calyx to the flowers or defer shed calyx. These decrease to be worth of the pear. In A Ke Su Stickup end fruits are more than Korla. Maybe In A Ke Su soils have lots of GA .they change pears’figure .
     4. The proportion of rough pear is 90% and 10% in internal and external shoots,The peoportion of fruit shapes is different too.The titration acid contents are high and the total soluble solid contents are low in rough pear.
引文
[1] 董存田.梨生产原理与技术[M].中国农业科技出版社.1995,146
    [2] 许方.梨树生物学.科学出版社[M]1992:1
    [3] 山东省莱阳农学院.梨[M].北京`科学出版社,1978:73~77
    [4] 陶世蓉.不同耐贮性梨果实的比较解剖.莱阳农学院学报[J],1992,9(3):183
    [5] 韦军,何风仁.酥梨.鸭梨石细胞群研究.江苏农学院学报[J],1988,9(1):35
    [6] 吴少华.梨果肉石细胞的研究.福建农业大学学报[J],1996,25(1):29~32
    [7] 刘庆华.梨果肉石细胞的形态结构与果实品质的关系.莱阳农学院学报[J],1992,9(4):252~255
    [8] Brewbaker J L. Biology of the angiosperm pollen graim [J].Indian Genet&planth Breed,1959,19:121 ~133
    [9] Geitmann A,et a1.Alterations in the actin cytoskeleton of pollen tubes are induced by the self-incomp- atibility reaction inPapaverrhoeas[J].Plant Cell,2000,12:1239~l251
    [10] Newbigin E,Anderson MA.,Clarke AE.Crametophytic self-incompatibility sys-tems[J].Plant cell, 1993,5: 1315~1324
    [11] Murfett J.,T.L.Athenon,B.Mou eta1..S-RNase exDressed in transgenic Nicotiana calluses S-allele specific polen rejection[J]. Nature,1994,367:563~566
    [12] Karunanandaa B,Huang S,Kao T-H,Carbohydrate moiety of the Petuia inflata S3 protein is not requ- ired for self-incompatibility interactions between pollen and pistil[J].Plant Cell,1994,6:1933~1940
    [13] Hiratsuka S , Kitoh Y, Matsumshima J.Induction of deformed pollentube tips and their morphological characteristics in self – incompatible Japanese pear[J ].J Japan Soc Hort Sci ,1991, 60:257~265
    [14] Zhang S L ,Hiratsuka S. Analysis of varietal differences in self-and cross incompatib- ility reactions of Japanese pear using stylar culture technique[J ].J Japan Soc Hort Sci,1999 , 68:373~383
    [15] 徐义流,张绍铃.梨配子体型自交不亲和性及其分子机理[J].果树学报,2003,20(1):59~63
    [16] Ishimizu T,Sato Y,Sato T,et a1.Identification and amino acid scquences of seven S—RNase assoc- iated with self-incongmtibifity of Japanese pear Pyruspyrifolia Nakai[J].J Biochem,1996,120:335~ 345
    [17] Roggen HP. van Dijk.A breaking incompatibility in Brassica oleracea L.by steel brash pollination. Euphytica. 1972,21:424~425
    [18] 马建江.巴州库尔勒香梨生产中存在的主要问题及解决办法[J].山西果树,1996(4):7~9
    [19] 王春芳.不同品种梨树高接香梨试验简报[J].北方果树,2001(4):40
    [20] 徐绍颖.植物生长调节剂与果树生产[M].上海科学技术出版社.1987
    [21] 宋清,岳长安,吴宪峰,张传州.砀山酥梨不脱萼果多的原因初探[J].落叶果树,2003.10
    [22] 周其石.花粉直感作用对香梨果实主要性状的影响[J].山西果树,1996(4):176~178
    [23] 徐庆岫.多效唑对香梨果形的影响[J].新疆农业科学,1991(4):172~174
    [24] 潘瑞炽,李 玲.植物生长发育的化学控制[M ].广州:广东高等教育出版社,1999:1~2
    [25] AMARJ IT S B. Plant grow th regulato rs in agriculture and ho rticulture [M]. NewYork, London,OX- ford: Food P roducts P ress,2000:1~16
    [26] Crane J C.Growth substances in setting and development[J].Annual Reviws of Plant Physiology,1964, 15:303~327 Nakai[J].J Biochem,1996,120:335~345
    [27] Addicott EF(1970) Plant hormones in the control of abscission[J].Biol Rev 45:485~524
    [28] Goffinet M C,Robinson T L,Lakso A N.Acomparison of ‘Empire’apple fruit size and anatomy in un- thinned and hand thinned trees [J].J.Hort Sci,1984,70(3):375~378
    [29] Monselise S P.Closing remarks.In Monselise S.P.(ed.)Hand Book of Fruit Set and Development [M]. CRC press,Florida,1986:521~537
    [30] Martin W R,Page J R W,Winflield M L,et al.Cotton fleahopper and associated microorganisms as components in the production of stress ethylene by cotton[J].Plant Physilogy,1988,87:280~283
    [31] Black B L,Bukovac M J,Hull J Jr.Effect of spray volume and time of NAA application on size and corpping of Redechief Delicious apple[J].Scientia Horticulturae,1995,64(5):53~57
    [32] Greene D W,Autio W R,Miller P.Thinning activity of benryladenine on several apple cultivars[J].J- ournal of the American Society for Horticultural Science,1990,115,64(4):253~264
    [33] Denny J O.Xenia includes metaxenia[J].Hort.Science,1992.27(7):722~728
    [34] 姜卫兵.植物生长调节剂对果实形状发育的影响[J].果树科学 1992,9(2):117~122
    [35] 陈昭存.葛 敏,吴邦良,等.果树植物生长调节剂在砀山酥梨上的应用[J].果农之友.2003(7):16
    [36] 饶景萍,任小林,童 斌.植物生长调节物质对果实生长发育的调控[J].西北植物学报.1998,报18(1):147~154
    [37] 刘 殊,何华平.CPPU对二宫白梨果实膨大的效应[J].西北农业大学学报.1997,25(4):65~67
    [38] 汪良驹,姜卫兵,刘晖,等.植物生长物质的 使用于绿色果品生产[J].园艺学报,2004,31(2): 259~262
    [39] 李学强,李作轩,李秀珍.CPPU对南果梨外观品质的影响[J].沈阳农业大学学报.2002,33(4): 252~254
    [40] Curry E.A et al:1986.Effect of paclobuttzal onfruit quanlity apple,pearand cheery[J].Acta Hortcultura- e,179:43~753
    [41] 薛桂新,吴秀玉,王 颖,等.赤霉素和多效唑对苹果果实纵横径和影响[J].延边大学农学报. 1999,21 (2):95~97
    [42] 王兴国,张淑敏,安兴云.影响苹果果形的因素.延边大学农学报.2001,23(4):257~271
    [43] 余前媛,植物化控在果树生产中的主要应用[J].北京农业科学.2000,18(6):26~27
    [44] 梁玉文,王嘉平.乙烯利、多效唑对幼旺树生长结果影响试验[J].北方园艺.1994,(5):32~33
    [45] 王 忠 主编.植物生理学[M].北京:中国农业出版社,2002:309
    [46] 亚合甫·木沙等:几种提高库尔勒香梨果实品质的技术研究[J].新疆农业科学 2007,44(S2): 108~112
    [47] 柴全喜,何新朝,宋素.梨果实品质低劣的原因及对策[J].烟台果树,2004,4:总72
    [48] 李 林,苏柳芸,井春芝,等.提高库尔勒香梨品质的重要途径[J].新疆农业科技.1993(3):25
    [49] 崔保红.库尔勒香梨在甘肃肃州区的引种表现[J].农业科技与信息.2005(9):24~25
    [50] Nasrallah M E.Genotype,protein,phenotype relationships in self-incompatibility of Brassica[J].Genet Res,1972,20:l5l~160
    [51] 俞世蓉.植物的自交不亲和性[J].生物学通报,1985,2:24~26
    [52] 张绍铃,平仲.徐国华,等.梨自交不亲和及其突变品种花柱内S4和S4SM基因的表达与作用比较[J].植物学报,2001,43(11):ll72~1178
    [53] 陈超,李保国,齐国辉,等果树自交不亲和机理及应用研究进展[J].河北林果研究,2005,(20): 86~90
    [54] 高新起,王秀玲.植物自交不亲和性[J].曲阜师范大学学报,1999,25(2):84~86
    [55] 孟金陵等.植物生殖遗传学[M].北京:科学出版社,1995
    [56] 高俊杰.用花粉提取液克服大白菜自交不亲和性研究[J].北方园艺,1997,(3):1~2
    [57] 王爱华,等果树自交不亲和性的研究进展[J].莱阳农学院园艺系,2002.(19):206~209
    [58] 李锡香.新鲜果蔬的品质及其分析法[M].北京:中国农业出版社,1994,14
    [59] 徐庆岫.授粉条件对香梨果实性状的影响[J].果树科学,1997,14(2):113~114
    [60] 赵昶灵,吴绍波.不同品种作授粉树对砀山梨果实品质的影响研究[J].落叶果树,1999,(4):14~ 15
    [61] 巩传银.梨果套袋综合效应技术[J].北方园艺,2002,(5):36
    [62] 黄新忠.梨实套袋效应试验[J].落叶果树,2000,(3):11~12
    [63] 张琦.套袋对库尔勒香梨果实品质的影响[J].北方园艺,2001,(5):10~11
    [64] 王大伟.塑料套袋对新疆库尔勒香梨果实品质的影响[J].北方园艺,2001,(6):18~19
    [65] 柴全喜.不同果袋对鸭梨套袋的效果[J].河北果树,2001,(1):7~8
    [66] 孟平红,吴康云,罗克明.不同处理对克服甘蓝自交不亲和性的效果的探讨[J].种子,2003,(1): 69
    [67] 张绍铃,房经贵,杨记磙.果树自交不亲和性的遗传与生理机制及其研究[J].果树学报, 2001, 18(1):49~52
    [68] 孙万仓,刘自刚,孟亚雄,等化学药剂处理克服芸芥自交不亲和性效果研究[J].中国油料作物学报, 2004, 26(1):8~11
    [69] 胡代泽,安彩泰,董惠珍,等用化学方法克服油菜自交不亲和性研究[J].中国油料,1983(2):1~ 5
    [70] 牛 宁,徐继忠,陈海江.梨自交不亲和性研究进展[J].河北林果研究,2006,(21):202~204
    [71] 刘宝敬,王晓佳.植物孢子体型自交不亲和性研究进展[J].生命科学,1996,8(4):38~41
    [72] 齐国辉.鸭梨自交不亲和与亲和变异的生理生化特性及分子机理研究[D].保定:河北农业大学, 2005
    [73] 张绍铃,周建涛,徐义流,等梨花柱半离体培养法及品种自交不亲合基因型鉴定[J].园艺学报,2003,30(6):703~706
    [74] 陈晓流,束怀瑞,陈学森,等核果类果树自交不亲和性研究进展[J].植物学通报,2004,21(6): 755~764
    [75] 薛勇彪,崔海洋,赖钊.不亲和性和自交亲和性及其分子生物学基础.北京:科学出版社,2002,128~148
    [76] 朱红祥.库尔勒香梨人工辅助授粉技术[J].园艺特产,2006,(4):31
    [77] 郭文场.库尔勒香梨[J].九州名产,2006
    [78] 吴绪兰.果树人工授粉怎样采花取粉[J].云南农业科技,2000,(3):45
    [79] 何标.梨树授粉技术要点[J].云南林业,2002,23(2):14
    [80] 黄国玺,牛鹏斐.梨花制粉和授粉的几种方法[J].烟台果树,2000,(2):47
    [81] 张大海.库尔勒香梨果形变化规律研究[J].新疆农业科学,1999(4):261~263
    [82] 李曙轩.植物生长调节剂与农业生产[M].科学出版社.1989.2:54~56
    [83] 李保国.库尔勒香梨果品品质的提高及其它管理(第五讲)[J].新疆农垦科技,2001(6):40~42
    [84] 廖明康.香梨品质及提高途径[J].山西果树,1989(9):12~13
    [85] 新疆农业科学院农科所,陕西省果树研究所主编.新疆的梨[M].新疆人民出版社.1978:21~22
    [86] 徐庆岫,盖新强.库尔勒香梨突顶果的发生原因及防治[J].新疆农业科学,1988,(2):113~114
    [87] 徐庆岫,盖新强,廖明康.库尔勒香梨果实生理病害发生规律及防治措施初探[J].新疆农业科学, 1987,(2):15~17
    [88] Lee,H.S.,S.Huang and T.-H.Kao.S Proteins control reiection of incompatiable pollen in Petunia inflata [J]. Nature,1994.367:560~563
    [89] 齐国辉.鸭梨自交不亲和与亲和变异的生理生化特性及分子机理研究[D].保定:河北农业大学, 2005
    [90] 孟金陵,刘定副,罗鹏等一植物生殖遗传学.1995,北京:科学出版社258~267
    [91] Nasrallah J B, Kao T H,Goldberg M L.A cDNA clone encoding an S-locus-specif glycoprotein from Brassica oleracea.Nature, 1985.318:263~267
    [92] Nasrallah J B, Nasrallah M E.Electrophoretic heterogeneity exhibited by the S-allele specific glycop- roteins of Brassica. Experimentia, 1984.40:270~281
    [93] Tatebe T, Studies on the physiological mechanism of self-incompatibility in Japanese radish Hort Sci. 1977.46:48~51
    [94] Pandey K.Overcoming incompatibility and promoting genetic recombination in flowering plants.Ne- w Zealand J.Bot, 1979.l7:645~664
    [95] Matsubara S, Overcoming self incompatibility by cytokinin treatment on Lilium longiflorum.Bot.Mag. (Tokyo) 1973, 86:43~46
    [96] Sharma N, Shivanna K.Lectin-like components of pollen and complementary sac charide moiety of the pistil are involved in self incompatibility recognition.Curr, Sci, 1983, 52::913~916
    [97] Kao T H ,McCubbin A G. How flowering plants discriminate between self and non - self pollen to pr- event inbreeding[J ] .Proc Nati Acad Sci USA , 1996 , 93:12059 ~12065
    [98] 张绍铃,平冢伸.梨花柱S 糖蛋白对离体花粉萌发及花粉管伸长的影响[J].园艺学报,2000, 27(4):251~256
    [99] Teh—hui Kao,Tatsuya,Tsukamoto.The molecular and genetic bases of S-Rnase-bsaedself-incompat- lbility.Plant Cell,2004,16(Supp1):72~83
    [100] LuuDT,QinK. LaublinG,et a1.Rejection of S-heteroallelic Pollen by a dualspecific S-RNasein Sol- anum chacoense predictsa multineric SI pollen component.Genetics,2001,159:329~335

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700