FTA滤膜用于基因芯片检测肉中常见食源性致病菌的研究
摘要
通过不对称PCR技术和基因芯片技术建立了一种准确、快速和高敏感性的检测肉中常见食源性致病菌的方法。用FTA滤膜从食品样品中直接提取模板DNA。用玻片作基因芯片的固相载体。选择常见的引起食物中毒的15株菌的16SrDNA基因的一个片段作为目的基因。设计这段基因的一对通用引物,下游引物用Cy5标记。对两轮不对称PCR反应体系中退火温度、Mg~(2+)浓度进行了优化,以确定适宜PCR反应体系和PCR扩增程序。第一轮适宜反应体系分别为:5uL的10×PCR buffer,0.5uL的20mmol/LdNTP,引物F1、R2各1uL(25umol/L),0.5uL的Taq酶(2.5U),含有基因组DNA模板的滤膜,用去离子水补足到50 uL。循环的条件为:94℃变性5min,从94℃变性30s、58.8℃退火1 min到72℃延伸30s共25个循环,最后72℃延伸5 min。第二轮适宜反应体系为:5uL的10×PCR buffer,0.5uL的20 mmol/L dNTP,引物R2 1uL(25umol/L),0.5uL的Taq酶(2.5U),第一轮的PCR产物1.5uL,用去离子水补足到50uL。循环的条件为:94℃变性5 min,从94℃变性30s、55.6℃退火30s到72℃延伸30s共30个循环,最后72℃延伸5min。设计二十五条种属的特异性探针,将它们氨基化修饰并点到玻片上,将不对称PCR的15种扩增产物与制作好的基因芯片杂交,用genepix4对杂交结果进行分析。
结果表明10株食源性致病菌(金黄色葡萄球菌、肉毒梭状芽孢杆菌、产气荚膜梭菌、宋内氏志贺氏菌、霍乱弧菌、普通变形杆菌、拟态弧菌、单核增生李斯特菌、小肠结肠炎耶尔森氏菌、腊样芽孢杆菌)的检测具有高敏感性和特异性;副溶血性弧菌的敏感性相对较低;河流弧菌同副溶血性弧菌存在很弱的错杂交,乙型溶血性链球菌的检测同单核增生李斯特菌和腊样芽孢杆菌存在很弱的错杂交,但是均不影响检测结果。大肠杆菌O157:H7和沙门氏菌由于同源性非常高,可以通过多重PCR方法检测出来。整个样品的检测过程耗时6h。本方法检测灵敏度是10~2CFU/mL。
针对在线BLAST的不足,建立了16SrDNA基因核苷酸序列本地数据库,并在此基础上进行寡核苷酸探针的本地BLAST以确保其特异性。通过与在线BLAST比较,本地BLAST在准确性、冗余信息的去除以及使用简便性等方面要优于在线BLAST。
基因芯片相比较其它检测方法有很大的优势,它不仅准确、快速、高效,而且高通量,可广泛应用于食源性致病菌感染的诊断、应对和控制。
A accurate rapid and sensitive method for detection for common foodborne pathogenic in food samples was established by using asy-PCR and DNA microarray technology.FTA Filter was used as template preparation for asy-PCR.Glass was used as the array support.A mutation region of the 16SrDNA gene was selected as the discrimination target from 15 species of bacteria causing foodborne infections.A pair of universal primers was designed for asy-PCR amplification of the 16SrDNA gene.The backward primer was labeled by Cy5.The PCR system was optimized including annealing temperature and the quantities of the Mg~(2+).The first reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer F1、R2(25umol/L),0.5uL Taq (2.5U)(TaKaRa),FTA filtering,42μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 58.8℃for 1min, extension at 72℃for 30s,for 25 cycles,and extension at 72℃for 5min.The second reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer R2(25umol/L),0.5uL Taq(2.5U)(TaKaRa),1.5uL product of the first PeR reaction, 43μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 55.6℃for 1min,extension at 72℃for 30s,for 25 cycles, and extension at 72℃for 5min.Twenty-five species(genera)-specific oligonucleotide detection probes were synthesized and spotted onto the glass.The 16SrDNA gene amplification products of 15 species of pathogenic bacteria were hybridized to the oligonucleotide array.Hybridization results were analyzed with genepix4.
ResuLts indicate that ten species of pathogenic bacteria(Staphylococcus aureus, Clostridium botuLinum,Clostridium perfringens,Shigella sonnei,Vibrio cholerae,Proteus vuLgaris,Vibrio mimicus,Listeria monocytogenes,Yersinia enterocolitica,Bacillus cereus) show high sensitivity and specificity for the oligonucleotide array;Vibrio parahaemolyticus showed low sensitivity for the oligonucleotide array;Vibrio fluvialis gave weak cross-reaction with Vibrio parahaemolyticus;Streptococcus hemolytic-βgave weak cross-reaction with Listeria monocytogenes and Bacillus cereus;but the reaction did not affect their detection.There is high identity between Escherichia coli O157:H7and Salmonella,they can be detected through muLtiplex PCR.The process of the detection took six hours from template preparation to the resuLt obtained.The sensitivity of the study was 10~2CFU/mL.
To overcome the shortages of on-line BLAST,the local nucleotide database of cry genes from Bt was established,and local BLAST was run based on it to ensure the specificity for each cry genes of oligonucleotide probes.By comparing local BLAST with on-line BLAST,advantages such as veracity,removing redundant and convenience were observed in local BLAST.
The superiority of oligonucleotide array over other tests lies on its accuracy,rapidity and efficiency in the diagnosis,treatment and control of foodborne infections.
结果表明10株食源性致病菌(金黄色葡萄球菌、肉毒梭状芽孢杆菌、产气荚膜梭菌、宋内氏志贺氏菌、霍乱弧菌、普通变形杆菌、拟态弧菌、单核增生李斯特菌、小肠结肠炎耶尔森氏菌、腊样芽孢杆菌)的检测具有高敏感性和特异性;副溶血性弧菌的敏感性相对较低;河流弧菌同副溶血性弧菌存在很弱的错杂交,乙型溶血性链球菌的检测同单核增生李斯特菌和腊样芽孢杆菌存在很弱的错杂交,但是均不影响检测结果。大肠杆菌O157:H7和沙门氏菌由于同源性非常高,可以通过多重PCR方法检测出来。整个样品的检测过程耗时6h。本方法检测灵敏度是10~2CFU/mL。
针对在线BLAST的不足,建立了16SrDNA基因核苷酸序列本地数据库,并在此基础上进行寡核苷酸探针的本地BLAST以确保其特异性。通过与在线BLAST比较,本地BLAST在准确性、冗余信息的去除以及使用简便性等方面要优于在线BLAST。
基因芯片相比较其它检测方法有很大的优势,它不仅准确、快速、高效,而且高通量,可广泛应用于食源性致病菌感染的诊断、应对和控制。
A accurate rapid and sensitive method for detection for common foodborne pathogenic in food samples was established by using asy-PCR and DNA microarray technology.FTA Filter was used as template preparation for asy-PCR.Glass was used as the array support.A mutation region of the 16SrDNA gene was selected as the discrimination target from 15 species of bacteria causing foodborne infections.A pair of universal primers was designed for asy-PCR amplification of the 16SrDNA gene.The backward primer was labeled by Cy5.The PCR system was optimized including annealing temperature and the quantities of the Mg~(2+).The first reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer F1、R2(25umol/L),0.5uL Taq (2.5U)(TaKaRa),FTA filtering,42μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 58.8℃for 1min, extension at 72℃for 30s,for 25 cycles,and extension at 72℃for 5min.The second reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer R2(25umol/L),0.5uL Taq(2.5U)(TaKaRa),1.5uL product of the first PeR reaction, 43μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 55.6℃for 1min,extension at 72℃for 30s,for 25 cycles, and extension at 72℃for 5min.Twenty-five species(genera)-specific oligonucleotide detection probes were synthesized and spotted onto the glass.The 16SrDNA gene amplification products of 15 species of pathogenic bacteria were hybridized to the oligonucleotide array.Hybridization results were analyzed with genepix4.
ResuLts indicate that ten species of pathogenic bacteria(Staphylococcus aureus, Clostridium botuLinum,Clostridium perfringens,Shigella sonnei,Vibrio cholerae,Proteus vuLgaris,Vibrio mimicus,Listeria monocytogenes,Yersinia enterocolitica,Bacillus cereus) show high sensitivity and specificity for the oligonucleotide array;Vibrio parahaemolyticus showed low sensitivity for the oligonucleotide array;Vibrio fluvialis gave weak cross-reaction with Vibrio parahaemolyticus;Streptococcus hemolytic-βgave weak cross-reaction with Listeria monocytogenes and Bacillus cereus;but the reaction did not affect their detection.There is high identity between Escherichia coli O157:H7and Salmonella,they can be detected through muLtiplex PCR.The process of the detection took six hours from template preparation to the resuLt obtained.The sensitivity of the study was 10~2CFU/mL.
To overcome the shortages of on-line BLAST,the local nucleotide database of cry genes from Bt was established,and local BLAST was run based on it to ensure the specificity for each cry genes of oligonucleotide probes.By comparing local BLAST with on-line BLAST,advantages such as veracity,removing redundant and convenience were observed in local BLAST.
The superiority of oligonucleotide array over other tests lies on its accuracy,rapidity and efficiency in the diagnosis,treatment and control of foodborne infections.
引文
[1]金大智,谢明杰,曹际娟.食品中单增李氏菌实时荧光PCR检测鉴定方法的建立.辽宁师范大学学报(自然科学版).2003.26(1):73-76.
[2]Giovanna franciosa et al.Characterization ofListeria monocytogenes Strains Involved in Invasive and Noninvasives Listeriosis Outbreaks by PCR-Based Fingerprinting Techniques Applied and environmental microbiology.2001 67(4):1793-1799.
[3]陈炳卿.食品污染与健康.化学工业出版社.2002.7.第一版:3-4.
[4]杨小兵.食品污染与安全控制.湖北预防医学杂志.2003.14(1):15-16.
[5]何欣荣.食品安全与食源性疾病控制研究进展.安徽预防医学杂志.2002.8(6):384-387.
[6]杨金萍.食品安全与食源性疾病.国外医学卫生学分册.2001.30(2):124-126.
[7]李业鹏.美国食品安全系统.中国食品卫生杂志.2001.13(4):44-49.
[8]Yang S.Augulo FC.Alterkruse SF.Evaluation of safe food-handling instructions on raw meat and poultry products.J Food Prot.2000.63(10):1321.
[9]卫生部.关于2000年全国重大事件中毒情况的通报.中国食品卫生杂志.2001.13(3):44.
[10]卫生部.关于2001年重大食物中毒情况的通报.中国食品卫生杂志.2002.14(3):62.
[11]宋明昌等.食品生物污染控制与检验.中国计量出版社.2001.3.第一版.67,107.
[12]《伯杰氏细菌鉴定手册》第八版 392-442,476-480.
[13]张河战.沙门氏菌的分类、命名及中国沙门氏菌菌型分布.微生物免疫学进展.2002.30(2):74-76.
[14]白薇,聂军,孙勇.空肠弯曲菌、沙门氏菌的16S rDNA的PCR-RDB检测方法的研究2001.17(1):33-35.
[15]齐素瑛.霍乱弧菌及其在食品中的检验.食品检验技术.1997.4:1-7.
[16]Riley L.W.,Remis R.S.,Helgeerson S.D.et al.Hemorrhagic colitis associated with a rare Escherichia coli serotype.N.Engl.J.Med.1983.308:681-685.
[17]Cahoon F.E.,Thompson J.S.,Frequency of Escherichia coli 0157:H7 isolation from stool specimens.Can.J.Microbiol.1987.33.:914-615.
[18]Marshall W.F.,Mclimans C.A.,Yu P.K.W.,Results of a 6-month survey of stool cultures for Escherichia coli 0157:H7.Mayo.Clin.Proc.1990.65:787-792.
[19]Thomas G.,Boyee M.B.,David I.,et al.Escherichia coli 0157:H7 and hemoiltic-uremic syndrome.N.Engl.J.Med.1995.333.:361-368.
[20]赵宝庆.番茄环斑病毒亲和诱捕PCR-ELISA和实时荧光PCR检测方法的建立.中国农业大学硕士论文.2001.
[21]寇运同,马洪明,刘晨光.用PCR技术快速检验食品中单核细胞增生性李斯特菌.食品科学.2001.22(5):52-55.
[22]韩伟,顾明.聚合酶链式反应与自动荧光免疫酶标检测弯度菌属的试验.2003.13(3):32-34.
[23]翁文川,覃文.联用VIDAS法和API Listeria法对食品中李斯特氏菌检验及分类.2000.21(4):59-62.
[24]曹泽虹,李勇.用PCR法快速测定食物中病毒原菌.微生物学通报.2001.28(4):73-76.
[25]William P.C.,Kelly M.P.,Morelli L et al.Selective Enumeration and Identification of Potentially Probiotic Lactobacillus and Bifidobacterium Species in Mined Bacterial Populations.International J.of Food Microbiology.1997.35(1):1-27
[26]Rudolf A,Gloeckner F.O.,Neef A.Modem Methods in Subsurface Microbiology.In situ Identification of Microorganisms with Nucleic Acid Probes.FEMS Microbiology Reviews.1997.20(3-4):191-200.
[27]Tortora G.J.,Funke B.R.Microbiology:An introduction.4th ed.Redwood City Calif.,the Beniamin/Gummings Publishing Company Inc.1992:262-269.
[28]Hazel D.M.,Kell D.B.Flow Cytometry and Sensitive Immunomagnetic Separation-PCR Method for the Detection of Escherichia coli 0157:H7 in Raw Milk and Ice Cream.J of Diary Research.1997.64(1):87-93.
[29]周帼萍,刘瑞祥.分子生物学技术在食品微生物快速鉴定中的应用.武汉食品工业学院学报.1999.1:36-38.
[30]谢晓红,陈悦,韩华忠.中国卫生检验杂志.2003.13(2):183.
[31]Schena M,Heller R.A,Theriault TP,Konrad K,Lachenmeier E,Davis RW.Microarrays:biotechnology's discovery platform for functional genomics.Trends Biotechnol 1998;16:301-306.
[32]Smith L,Underhill P,Pritchard C,Tymowska-Lalanne Z,Abdul-Hussein S,Hilton H,Winchester L,Williams D,Freeman T,Webb S,Greenfield A.Single primer amplification(SPA)of cDNA for microarray expression analysis.Nucleic Acids Res 2003;31:e9.
[33]Welford S M,Gregg J,Chen E et al.,Detection of differentially expressed genes in primary tumor tissues using representationl differences analysis coupled to microarray hybridization.Nucleic Acids Research,1998,26(12):3059-3062
[34]Pease,A.C.,D.Solas,E.J.Sullivan,M.T.Cronin,C.P.Holmes,andS.R.A.Fodor.Light-generated oligonucleotide arrays for rapid DNA sequcnce analysis.Proc.Natl.Acad.Sci.USA 1994.91:5022-5026.
[35]LipshutZ,R.J.,5.P.A.Fodor,T.R.Gingeras,and D.J.Locart.High-density synthetic oligonucleotide arrays.Nature Genet.1999.21:20-24.
[36]Beattie WG,Meng L,Turner SL,Varma RS,Dao DD,Beattie KL.Hybridization of DNA targets to glass-tethered oligonucleotide probes.Mol Biotechnol.1995;4(3):213-25.
[37]冯永强,阎小君,基因芯片技术,国外医学:分子生物学分册.2000,22(1).1-5
[38]Hoheisel,J.D.oligomer-chip technology.Tibtech.1997.15:465-469.
[39]Zhou JZ,Davey ME,Figueras JB,Rivkina E,Glichinsky D,Tiedje JM:Phylogenetic diversity of a bacterial community determined from Siberian tundra soil DNA.Microbiology 1997,143:3913-3919.
[40]Voordouw G,Shen Y,Harrington CS,Telang AJ,Jack TR,Westlake DWS:Quantitative reverse sample genome probing of microbial communities and its application to oil field production waters.Appl Environ Microbiol 1993,59:4101-4114.
[41]Collins,M.L.,Irvine,B.,Tyner,D.,et al.,A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/mL.Nucleic Acid Res.1997,25,2979-2984.
[42]Schweitzer B,Wiitshire S,Lambert J,O'Malley S,Kukanskis K,Zhu Z,Kingsmore SF,Lizardi PM,Ward DC.Inaugural article:immunoassays with rolling circle DNA amplification:a versatile platform for ultrasensitive antigen detection.Proc Natl Acad Sci U S A 2000;97:10113-10119.
[43]Hoque MO,Lee CC,Cairns P,Schoenberg M,Sidransky D.Genome-wide genetic characterization of bladder cancer:a comparison of high-density single-nucleotide polymorphism arrays and PCR-based microsatellite analysis.Cancer Res 2003;63:2216-2222.
[44]Mantripragada KK,Tapia-Paez I,Blennow E,Nilsson P,Wedell A,Dumanski JP.DNA copy-number analysis of the 22q11 deletion-syndrome region using array-CGH with genomic and PCR-based targets.Int J Mol Med 2004;13:273-279.
[45]Mitterer G,Huber M,Leidinger E,Kirisits C,Lubitz W,Mueller MW,Schmidt WM.Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences.J Clin Microbiol 2004;42:1048-1057.
[46]Sun ZH,Zheng WL,Mao XM,Zhang B,Lu L,Ma XD,Shi R,Ma WL.Rapid preparation of DNA microarray using PCR for hepatitis B and D virus detection.Di Yi Jun Yi Da Xue Xue Bao 2003;23:677-679.
[47]Wu CF,Valdes JJ,Bentley WE,Sekowski JW:DNA microarray for discrimination between pathogenic 0157:H7 EDL933 and non-pathogenic Escherichia coli strains.Biosens.Bioelectron.2003,19:1-8.
[48]Jae-Chang Cho,James M,Tiedje.Quantitative Detection of Microbial Genes by Using DNA Microarrays.Appl.Environ.Microbiol.2002.68(3),1425-1430
[49]Lee GP,Min BE,Kim CS,Choi SH,Ham CH,Kim SU,Ryu KH.Plant virus cDNA chip hybridization for detection and differentiation of four cucurbit-infecting Tobamoviruses.J Virol Methods SD 2003;110:19-24.
[50]Boonham N,Walsh K,Smith P,Madagan K,Graham I,Barker I.Detection of potato viruses using microarray technology:towards a generic method for plant viral disease diagnosis.J Virol Methods 2003;108:181-187.
[51]Kawaguchi K,Kaneko S,Honda M,Kawai HF,Shirota Y,Kobayashi K.Detection of hepatitis B virus DNA in sera from patients with chronic hepatitis B virus infection by DNA microarray method.J Clin Microbiol 2003;41:1701-1704.
[52]吴海,顾少华,胡志前,毛裕民等.检测乙、丙型肝炎病毒基因芯片的制备.第二军医大学学报2001,22(6),527-529.
[53]Bekal S,Brousseau R,Masson L,Prefontaine G,Fairbrother J,Harel J.Rapid identification of Escherichia coli pathotypes by virulence gene detection with DNA microarrays.J Clin Microbiol 2003;41:2113-2125.
[54]Alsaker KV,Spitzer TR,Papoutsakis ET.Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress.J Bacteriol 2004;186:1959-1971.
[55]翟俊辉,郭兆彪,宋亚军等.16 S rDNA基因芯片检测临床常见感染性细菌.临床检验杂志2002,20(3),133-136.
[56] Murray. A. E, Lies. D., G. Li, Nealson. K., et al., DNA/DNA hybridization to microarrays reveals gene-specific differences between closely related microbial genomes. PNAS 2001. 98 (17) 9853-9858
[57] Dong G, Loukinova E, Chen Z, Gangi L, Chanturita TI, Liu ET, Van Waes C. Molecular profiling of transformed and metastatic murine squamous carcinoma cells by differential display and cDNA microarray reveals altered expression of multiple genes related to growth, apoptosis, angiogenesis, and the NF-kappaB signal pathway. Cancer Res 2001; 61:4797-4808.
[58] Salama N., Guillemin K., Timothy K. M., et al, A whole genome microarray reveals genetic diversity among Helicobacter pylori strains. PNAS 2000.97 (26), 14668-14673
[59] Chandler DP, Newton GJ, Small JA, Daly DS. Sequence versus structure for the direct detecfion of 16S rRNA on planar oligonucleotide microarrays. Appl Environ Microbiol 2003; 69:2950-2958.
[60] Chizhikov V, Rasooly A, and Chumakov K, Levy DD: Microarray analysis of microbial virulence factors. Appl.Environ.Microbiol. 2001,67:3258-3263.
[61] de Saizieu A, Certa U, Warrington J, Gray C, Keck W, Mous J. Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays. Nat Biotechnol 1998; 16:45-48.
[62] Guschin DY, Mobarry BK, Proudnikov D, Stahl DA, Rittmann BE, Mirzabekov AD. Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology. Appl Environ Microbiol 1997; 63:2397-2402.
[63] Hwang TS, Jeong JK, Park M, Han HS, Choi HK, Park TS. Detection and typing of HPV genotypes in various cervical lesions by HPV oligonucleotide microarray. Gynecol Oncol SD 2003; 90:51-56.
[64] Kane MD, Jatkoe TA, Stumpf CR, Lu J, Thomas JD, Madore SJ. Assessment of the sensitivity and specificity of oligonucleotide (50mer) microarrays. Nucleic Acids Res 2000; 28:4552-4557.
[65] Laassri M, Chizhikov V, Mikheev M, Shchelkunov S, Chumakov K. Detection and discrimination of orthopoxviruses using microarrays of immobilized oligonucleotides. J Virol Methods 2003; 112:67-78.
[66] Loy A, Lehner A, Lee N, Adamczyk J, Meier H, Ernst J, Schleifer KH, Wagner M. Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment. Appl Environ Microbiol 2002; 68:5064-5081.
[67] Peplies J, Glockner FO, Amann R. Optimization strategies for DNA microarray-based detection of bacteria with 16S rRNA-targeting oligonucleotide probes. Appl Environ Microbiol 2003; 69:1397-1407.
[68] Perrin A, Duracher D, Perret M, Cleuziat P, Mandrand B. A combined oligonucleotide and protein microarray for the codetection of nucleic acids and antibodies associated with human immunodeficiency virus, hepatitis B virus, and hepatitis C virus infections. Anal Biochem SD 2003; 322:148-155.
[69] Small J, Call DR, Brockman FJ, Straub TM, Chandler DP. Direct detection of 16S rRNA in soil extracts by using oligonucleotide microarrays.Appl Environ Microbiol 2001;67:4708-4716.
[70]Wang RF,Beggs ML,Robertson LH,Cerniglia CE.Design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples.FEMS Microbiol Lett 2002;213:175-182.
[71]范保星,刘又宁,孟凡义等.SARS病毒全基因组芯片在临床中的应用,解放军医学杂志,2003,28(11)998-1000
[72]周琦,赖平安,汪琳等,基因芯片技术快速检测SARS病毒,检验检疫科学,2003,13(5)33-36.
[73]Taroncher-Oldenburg G,Griner EM,Francis CA,Ward BB.Oligonucleotide microarray for the study of functional gene diversity in the nitrogen cycle in the environment.Appl Environ Microbiol 2003;69:1159-1171.
[74]Wilson WJ,Strout CL,DeSantis TZ,Stilwell JL,Carrano AV,Andersen GL.Sequence-specific identification of 18 pathogenic microorganisms using microarray technology.Mol Cell Probes 2002;16:119-127.
[75]Gingeras.T.R,Ghassan G.,Eugene W.,et al.,Simultaneous Genotyping and Species Identification Using Hybridization Pattern Recognition Analysis of Generic Mycobacterium DNA Arrays.Genome Research.1998.8:435-448
[76]Troesch.A.,H.Nguyen,C.G.Miyada,S.Desvarenne,T.R.Gingeras,P.M.Kaplan,P.Cros,C.Mabilat.Mycobacterium Species Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays.Journal Of Clinical Microbiology,1999.37:49-55
[77]Call DR,Brockman F J,Chandler DP.Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays.Int J Food Microbiol 2001;67:71-80.
[78]Cho NH,An HJ,Jeong JK,Kang S,Kim JW,Kim YT,Park TK.Genotyping of 22 human papillomavirus types by DNA chip in Korean women:comparison with cytologic diagnosis.Am J Obstet Gynecol 2003;188:56-62.
[79]Kim CJ,Jeong JK,Park M,Park TS,Park TC,Namkoong SE,Park JS.HPV oligonucleotide microarray-based detection of HPV genotypes in cervical neoplastic lesions.Gynecol Oncol 2003;89:210-217.
[80]吴力游 建立用于环境及土壤微生物群落分析的基因芯片技术(博士论文)湖南农业大学2001 50-63
[81]Kaferstein,E,Abdussalam,M.,Food safety in the 21st century.BuLl[J].World Health Organ.1999,77,347-351.
[82]Bresee,J.S.,Widdowson,M.A.,Monroe,S.S.et al.Foodbome viral gastroenteritis:challenges and opportunities[J].Clin.Infect.Dis.2002,35,748-753.
[83]Daniels,N.A.,MacKinnon,L.,Rowe,S.M.et al.Foodborne disease outbreaks in United States schools[J].Pediatr.Infect.Dis.J.2002.21,623-628.
[84]Mead,P.S.,Siutsker,L.,Dietz,V.,et al.Food-related illness and death in the United States[J]. Emerg. Infect. Dis. 1999, 5, 607-625.
[85] Lee, W.C., Lee, M.J., Kim, J.S. et al. Foodborne illness outbreaks in Korea and Japan studied retrospectively [J]. J. FoodProt. 2001,64, 899-902.
[86] Helps, C.R., Harbour, D.A., Corry, J.E. PCR-based 16S ribosomal DNA detection technique for Clostridium estertheticum causing spoilage in vacuum-packed chill-stored beef [J]. Int. J.Food Microbiol. 1999,52,57-65.
[87] Matar, G.M., Koehler, J.E., Malcolm, G. et al. Identification of Bartonella species directly in clinical specimens by PCR-restriction fragment length polymorphism analysis of a 16S rRNA gene fragment [J]. J. Clin. Microbiol. 1999,37,4045-4047.
[88] Siqueira, J.F., Rocas, I.N., Favieri, A. et al. Detection of Treponema denticola in endodontic infections by 16SrRNA gene-directed polymerase chain reaction [J]. Oral Microbiol.Immunol. 2000,15,335-337.
[89] Smith, J.G, Kong, L., Abruzzo, G.K. et al. PCR detection of colonization by Helicobacter pylori in conventional, euthymic mice based on the 16S ribosomal gene sequence [J]. Clin. Diagn. Lab. Immunol. 1996,3,66-72.
[90] Frahm, E., Heiber, I., Hoffmann, S. et al. Application of 23S rDNA-targeted oligonucleotide probes specific for enterococcito water hygiene control [J]. Appl. Microbiol. 1998,21,450- 453.
[91] Straub, J.A., Hertel, C, Hammes, W.P. A 23S rDNA-targeted polymerase chain reaction-based system for detection of Staphylococcus aureus in meat starter cuLtures and dairy products [J] . J. Food Prot. 1999, 62, 1150- 1156.
[92] Tesfaye, M., Holl, F.B. Group-specific differentiation of Rhizobium from clover species by PCR amplification of 23SrDNA sequences [J]. Can. J. Microbiol. 1998,44,1102- 1105.
[93] Hamid, M.E., Roth, A., Landt, O. et al. Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense species based on 16S-23S ribosomal DNA internal transcribed spacer sequences [J]. J. Clin. Microbiol. 2002,40, 707-711.
[94] Gu, X.X., Rossau, R., Jannes, G. et al. The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay [J]. Microbiol. 1998, 144, 1013-1019.
[95] Madico, G, Quinn, T.C., Boman, J. et al. Touchdown enzyme time release-PCR for detection and identification of Chlamydia trachomatis, C. pneumoniae, and C. psittaci using the 16S and 16S-23S spacer rRNA genes [J]. J. Clin. Microbiol. 2000,38,1085- 1093.
[96] Sasaki, Y., Yamamoto, K., Amimoto, K. et al. Amplification of the 16S-23S rDNA spacer region for rapid detection of Clostridiumchauvoei and Clostridium septicum [J]. Res. Vet. Sci. 2001, 71,227-229
[97]Brousseau,R.,Hill,J.E.,Prefontaine,G.et al.Streptococcus suis serotypes characterized by analysis ofchaperonin 60 gene sequences[J].Appl.Environ.Microbiol.2001,67,4828-4833.
[98]Kwok,A.Y.,Su,S.C.,Reynolds,R.P.et al.Species identification and hylogenetic relationships based on partial HSP60 gene sequences within the genus Staphylococcus[J].Int.J.Syst.Bacteriol.1999,49,1181-1192.
[99]Marston,E.L.,Summer,J.W.,Regnery,R.L.Evaluation of intraspecies genetic variation within the 60kDa heat-shock protein gene(groEL)of Bartonella species[J].Int.J.Syst.Bacteriol.,1999,49,1015-1023.
[100]Wang,R.F.,Beggs,M.L.,Robertson,L.H.et al.Design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples [J].FEMS Microbiol.Lett.2002,213,175-182.
[101]刘景武,张伟,何俊萍,周巍等,FTA滤膜用于PCR检测肉中的金黄色葡萄球菌研究[J].生物工程学报,2005.21(6),1009-1013
[2]Giovanna franciosa et al.Characterization ofListeria monocytogenes Strains Involved in Invasive and Noninvasives Listeriosis Outbreaks by PCR-Based Fingerprinting Techniques Applied and environmental microbiology.2001 67(4):1793-1799.
[3]陈炳卿.食品污染与健康.化学工业出版社.2002.7.第一版:3-4.
[4]杨小兵.食品污染与安全控制.湖北预防医学杂志.2003.14(1):15-16.
[5]何欣荣.食品安全与食源性疾病控制研究进展.安徽预防医学杂志.2002.8(6):384-387.
[6]杨金萍.食品安全与食源性疾病.国外医学卫生学分册.2001.30(2):124-126.
[7]李业鹏.美国食品安全系统.中国食品卫生杂志.2001.13(4):44-49.
[8]Yang S.Augulo FC.Alterkruse SF.Evaluation of safe food-handling instructions on raw meat and poultry products.J Food Prot.2000.63(10):1321.
[9]卫生部.关于2000年全国重大事件中毒情况的通报.中国食品卫生杂志.2001.13(3):44.
[10]卫生部.关于2001年重大食物中毒情况的通报.中国食品卫生杂志.2002.14(3):62.
[11]宋明昌等.食品生物污染控制与检验.中国计量出版社.2001.3.第一版.67,107.
[12]《伯杰氏细菌鉴定手册》第八版 392-442,476-480.
[13]张河战.沙门氏菌的分类、命名及中国沙门氏菌菌型分布.微生物免疫学进展.2002.30(2):74-76.
[14]白薇,聂军,孙勇.空肠弯曲菌、沙门氏菌的16S rDNA的PCR-RDB检测方法的研究2001.17(1):33-35.
[15]齐素瑛.霍乱弧菌及其在食品中的检验.食品检验技术.1997.4:1-7.
[16]Riley L.W.,Remis R.S.,Helgeerson S.D.et al.Hemorrhagic colitis associated with a rare Escherichia coli serotype.N.Engl.J.Med.1983.308:681-685.
[17]Cahoon F.E.,Thompson J.S.,Frequency of Escherichia coli 0157:H7 isolation from stool specimens.Can.J.Microbiol.1987.33.:914-615.
[18]Marshall W.F.,Mclimans C.A.,Yu P.K.W.,Results of a 6-month survey of stool cultures for Escherichia coli 0157:H7.Mayo.Clin.Proc.1990.65:787-792.
[19]Thomas G.,Boyee M.B.,David I.,et al.Escherichia coli 0157:H7 and hemoiltic-uremic syndrome.N.Engl.J.Med.1995.333.:361-368.
[20]赵宝庆.番茄环斑病毒亲和诱捕PCR-ELISA和实时荧光PCR检测方法的建立.中国农业大学硕士论文.2001.
[21]寇运同,马洪明,刘晨光.用PCR技术快速检验食品中单核细胞增生性李斯特菌.食品科学.2001.22(5):52-55.
[22]韩伟,顾明.聚合酶链式反应与自动荧光免疫酶标检测弯度菌属的试验.2003.13(3):32-34.
[23]翁文川,覃文.联用VIDAS法和API Listeria法对食品中李斯特氏菌检验及分类.2000.21(4):59-62.
[24]曹泽虹,李勇.用PCR法快速测定食物中病毒原菌.微生物学通报.2001.28(4):73-76.
[25]William P.C.,Kelly M.P.,Morelli L et al.Selective Enumeration and Identification of Potentially Probiotic Lactobacillus and Bifidobacterium Species in Mined Bacterial Populations.International J.of Food Microbiology.1997.35(1):1-27
[26]Rudolf A,Gloeckner F.O.,Neef A.Modem Methods in Subsurface Microbiology.In situ Identification of Microorganisms with Nucleic Acid Probes.FEMS Microbiology Reviews.1997.20(3-4):191-200.
[27]Tortora G.J.,Funke B.R.Microbiology:An introduction.4th ed.Redwood City Calif.,the Beniamin/Gummings Publishing Company Inc.1992:262-269.
[28]Hazel D.M.,Kell D.B.Flow Cytometry and Sensitive Immunomagnetic Separation-PCR Method for the Detection of Escherichia coli 0157:H7 in Raw Milk and Ice Cream.J of Diary Research.1997.64(1):87-93.
[29]周帼萍,刘瑞祥.分子生物学技术在食品微生物快速鉴定中的应用.武汉食品工业学院学报.1999.1:36-38.
[30]谢晓红,陈悦,韩华忠.中国卫生检验杂志.2003.13(2):183.
[31]Schena M,Heller R.A,Theriault TP,Konrad K,Lachenmeier E,Davis RW.Microarrays:biotechnology's discovery platform for functional genomics.Trends Biotechnol 1998;16:301-306.
[32]Smith L,Underhill P,Pritchard C,Tymowska-Lalanne Z,Abdul-Hussein S,Hilton H,Winchester L,Williams D,Freeman T,Webb S,Greenfield A.Single primer amplification(SPA)of cDNA for microarray expression analysis.Nucleic Acids Res 2003;31:e9.
[33]Welford S M,Gregg J,Chen E et al.,Detection of differentially expressed genes in primary tumor tissues using representationl differences analysis coupled to microarray hybridization.Nucleic Acids Research,1998,26(12):3059-3062
[34]Pease,A.C.,D.Solas,E.J.Sullivan,M.T.Cronin,C.P.Holmes,andS.R.A.Fodor.Light-generated oligonucleotide arrays for rapid DNA sequcnce analysis.Proc.Natl.Acad.Sci.USA 1994.91:5022-5026.
[35]LipshutZ,R.J.,5.P.A.Fodor,T.R.Gingeras,and D.J.Locart.High-density synthetic oligonucleotide arrays.Nature Genet.1999.21:20-24.
[36]Beattie WG,Meng L,Turner SL,Varma RS,Dao DD,Beattie KL.Hybridization of DNA targets to glass-tethered oligonucleotide probes.Mol Biotechnol.1995;4(3):213-25.
[37]冯永强,阎小君,基因芯片技术,国外医学:分子生物学分册.2000,22(1).1-5
[38]Hoheisel,J.D.oligomer-chip technology.Tibtech.1997.15:465-469.
[39]Zhou JZ,Davey ME,Figueras JB,Rivkina E,Glichinsky D,Tiedje JM:Phylogenetic diversity of a bacterial community determined from Siberian tundra soil DNA.Microbiology 1997,143:3913-3919.
[40]Voordouw G,Shen Y,Harrington CS,Telang AJ,Jack TR,Westlake DWS:Quantitative reverse sample genome probing of microbial communities and its application to oil field production waters.Appl Environ Microbiol 1993,59:4101-4114.
[41]Collins,M.L.,Irvine,B.,Tyner,D.,et al.,A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/mL.Nucleic Acid Res.1997,25,2979-2984.
[42]Schweitzer B,Wiitshire S,Lambert J,O'Malley S,Kukanskis K,Zhu Z,Kingsmore SF,Lizardi PM,Ward DC.Inaugural article:immunoassays with rolling circle DNA amplification:a versatile platform for ultrasensitive antigen detection.Proc Natl Acad Sci U S A 2000;97:10113-10119.
[43]Hoque MO,Lee CC,Cairns P,Schoenberg M,Sidransky D.Genome-wide genetic characterization of bladder cancer:a comparison of high-density single-nucleotide polymorphism arrays and PCR-based microsatellite analysis.Cancer Res 2003;63:2216-2222.
[44]Mantripragada KK,Tapia-Paez I,Blennow E,Nilsson P,Wedell A,Dumanski JP.DNA copy-number analysis of the 22q11 deletion-syndrome region using array-CGH with genomic and PCR-based targets.Int J Mol Med 2004;13:273-279.
[45]Mitterer G,Huber M,Leidinger E,Kirisits C,Lubitz W,Mueller MW,Schmidt WM.Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences.J Clin Microbiol 2004;42:1048-1057.
[46]Sun ZH,Zheng WL,Mao XM,Zhang B,Lu L,Ma XD,Shi R,Ma WL.Rapid preparation of DNA microarray using PCR for hepatitis B and D virus detection.Di Yi Jun Yi Da Xue Xue Bao 2003;23:677-679.
[47]Wu CF,Valdes JJ,Bentley WE,Sekowski JW:DNA microarray for discrimination between pathogenic 0157:H7 EDL933 and non-pathogenic Escherichia coli strains.Biosens.Bioelectron.2003,19:1-8.
[48]Jae-Chang Cho,James M,Tiedje.Quantitative Detection of Microbial Genes by Using DNA Microarrays.Appl.Environ.Microbiol.2002.68(3),1425-1430
[49]Lee GP,Min BE,Kim CS,Choi SH,Ham CH,Kim SU,Ryu KH.Plant virus cDNA chip hybridization for detection and differentiation of four cucurbit-infecting Tobamoviruses.J Virol Methods SD 2003;110:19-24.
[50]Boonham N,Walsh K,Smith P,Madagan K,Graham I,Barker I.Detection of potato viruses using microarray technology:towards a generic method for plant viral disease diagnosis.J Virol Methods 2003;108:181-187.
[51]Kawaguchi K,Kaneko S,Honda M,Kawai HF,Shirota Y,Kobayashi K.Detection of hepatitis B virus DNA in sera from patients with chronic hepatitis B virus infection by DNA microarray method.J Clin Microbiol 2003;41:1701-1704.
[52]吴海,顾少华,胡志前,毛裕民等.检测乙、丙型肝炎病毒基因芯片的制备.第二军医大学学报2001,22(6),527-529.
[53]Bekal S,Brousseau R,Masson L,Prefontaine G,Fairbrother J,Harel J.Rapid identification of Escherichia coli pathotypes by virulence gene detection with DNA microarrays.J Clin Microbiol 2003;41:2113-2125.
[54]Alsaker KV,Spitzer TR,Papoutsakis ET.Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress.J Bacteriol 2004;186:1959-1971.
[55]翟俊辉,郭兆彪,宋亚军等.16 S rDNA基因芯片检测临床常见感染性细菌.临床检验杂志2002,20(3),133-136.
[56] Murray. A. E, Lies. D., G. Li, Nealson. K., et al., DNA/DNA hybridization to microarrays reveals gene-specific differences between closely related microbial genomes. PNAS 2001. 98 (17) 9853-9858
[57] Dong G, Loukinova E, Chen Z, Gangi L, Chanturita TI, Liu ET, Van Waes C. Molecular profiling of transformed and metastatic murine squamous carcinoma cells by differential display and cDNA microarray reveals altered expression of multiple genes related to growth, apoptosis, angiogenesis, and the NF-kappaB signal pathway. Cancer Res 2001; 61:4797-4808.
[58] Salama N., Guillemin K., Timothy K. M., et al, A whole genome microarray reveals genetic diversity among Helicobacter pylori strains. PNAS 2000.97 (26), 14668-14673
[59] Chandler DP, Newton GJ, Small JA, Daly DS. Sequence versus structure for the direct detecfion of 16S rRNA on planar oligonucleotide microarrays. Appl Environ Microbiol 2003; 69:2950-2958.
[60] Chizhikov V, Rasooly A, and Chumakov K, Levy DD: Microarray analysis of microbial virulence factors. Appl.Environ.Microbiol. 2001,67:3258-3263.
[61] de Saizieu A, Certa U, Warrington J, Gray C, Keck W, Mous J. Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays. Nat Biotechnol 1998; 16:45-48.
[62] Guschin DY, Mobarry BK, Proudnikov D, Stahl DA, Rittmann BE, Mirzabekov AD. Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology. Appl Environ Microbiol 1997; 63:2397-2402.
[63] Hwang TS, Jeong JK, Park M, Han HS, Choi HK, Park TS. Detection and typing of HPV genotypes in various cervical lesions by HPV oligonucleotide microarray. Gynecol Oncol SD 2003; 90:51-56.
[64] Kane MD, Jatkoe TA, Stumpf CR, Lu J, Thomas JD, Madore SJ. Assessment of the sensitivity and specificity of oligonucleotide (50mer) microarrays. Nucleic Acids Res 2000; 28:4552-4557.
[65] Laassri M, Chizhikov V, Mikheev M, Shchelkunov S, Chumakov K. Detection and discrimination of orthopoxviruses using microarrays of immobilized oligonucleotides. J Virol Methods 2003; 112:67-78.
[66] Loy A, Lehner A, Lee N, Adamczyk J, Meier H, Ernst J, Schleifer KH, Wagner M. Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment. Appl Environ Microbiol 2002; 68:5064-5081.
[67] Peplies J, Glockner FO, Amann R. Optimization strategies for DNA microarray-based detection of bacteria with 16S rRNA-targeting oligonucleotide probes. Appl Environ Microbiol 2003; 69:1397-1407.
[68] Perrin A, Duracher D, Perret M, Cleuziat P, Mandrand B. A combined oligonucleotide and protein microarray for the codetection of nucleic acids and antibodies associated with human immunodeficiency virus, hepatitis B virus, and hepatitis C virus infections. Anal Biochem SD 2003; 322:148-155.
[69] Small J, Call DR, Brockman FJ, Straub TM, Chandler DP. Direct detection of 16S rRNA in soil extracts by using oligonucleotide microarrays.Appl Environ Microbiol 2001;67:4708-4716.
[70]Wang RF,Beggs ML,Robertson LH,Cerniglia CE.Design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples.FEMS Microbiol Lett 2002;213:175-182.
[71]范保星,刘又宁,孟凡义等.SARS病毒全基因组芯片在临床中的应用,解放军医学杂志,2003,28(11)998-1000
[72]周琦,赖平安,汪琳等,基因芯片技术快速检测SARS病毒,检验检疫科学,2003,13(5)33-36.
[73]Taroncher-Oldenburg G,Griner EM,Francis CA,Ward BB.Oligonucleotide microarray for the study of functional gene diversity in the nitrogen cycle in the environment.Appl Environ Microbiol 2003;69:1159-1171.
[74]Wilson WJ,Strout CL,DeSantis TZ,Stilwell JL,Carrano AV,Andersen GL.Sequence-specific identification of 18 pathogenic microorganisms using microarray technology.Mol Cell Probes 2002;16:119-127.
[75]Gingeras.T.R,Ghassan G.,Eugene W.,et al.,Simultaneous Genotyping and Species Identification Using Hybridization Pattern Recognition Analysis of Generic Mycobacterium DNA Arrays.Genome Research.1998.8:435-448
[76]Troesch.A.,H.Nguyen,C.G.Miyada,S.Desvarenne,T.R.Gingeras,P.M.Kaplan,P.Cros,C.Mabilat.Mycobacterium Species Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays.Journal Of Clinical Microbiology,1999.37:49-55
[77]Call DR,Brockman F J,Chandler DP.Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays.Int J Food Microbiol 2001;67:71-80.
[78]Cho NH,An HJ,Jeong JK,Kang S,Kim JW,Kim YT,Park TK.Genotyping of 22 human papillomavirus types by DNA chip in Korean women:comparison with cytologic diagnosis.Am J Obstet Gynecol 2003;188:56-62.
[79]Kim CJ,Jeong JK,Park M,Park TS,Park TC,Namkoong SE,Park JS.HPV oligonucleotide microarray-based detection of HPV genotypes in cervical neoplastic lesions.Gynecol Oncol 2003;89:210-217.
[80]吴力游 建立用于环境及土壤微生物群落分析的基因芯片技术(博士论文)湖南农业大学2001 50-63
[81]Kaferstein,E,Abdussalam,M.,Food safety in the 21st century.BuLl[J].World Health Organ.1999,77,347-351.
[82]Bresee,J.S.,Widdowson,M.A.,Monroe,S.S.et al.Foodbome viral gastroenteritis:challenges and opportunities[J].Clin.Infect.Dis.2002,35,748-753.
[83]Daniels,N.A.,MacKinnon,L.,Rowe,S.M.et al.Foodborne disease outbreaks in United States schools[J].Pediatr.Infect.Dis.J.2002.21,623-628.
[84]Mead,P.S.,Siutsker,L.,Dietz,V.,et al.Food-related illness and death in the United States[J]. Emerg. Infect. Dis. 1999, 5, 607-625.
[85] Lee, W.C., Lee, M.J., Kim, J.S. et al. Foodborne illness outbreaks in Korea and Japan studied retrospectively [J]. J. FoodProt. 2001,64, 899-902.
[86] Helps, C.R., Harbour, D.A., Corry, J.E. PCR-based 16S ribosomal DNA detection technique for Clostridium estertheticum causing spoilage in vacuum-packed chill-stored beef [J]. Int. J.Food Microbiol. 1999,52,57-65.
[87] Matar, G.M., Koehler, J.E., Malcolm, G. et al. Identification of Bartonella species directly in clinical specimens by PCR-restriction fragment length polymorphism analysis of a 16S rRNA gene fragment [J]. J. Clin. Microbiol. 1999,37,4045-4047.
[88] Siqueira, J.F., Rocas, I.N., Favieri, A. et al. Detection of Treponema denticola in endodontic infections by 16SrRNA gene-directed polymerase chain reaction [J]. Oral Microbiol.Immunol. 2000,15,335-337.
[89] Smith, J.G, Kong, L., Abruzzo, G.K. et al. PCR detection of colonization by Helicobacter pylori in conventional, euthymic mice based on the 16S ribosomal gene sequence [J]. Clin. Diagn. Lab. Immunol. 1996,3,66-72.
[90] Frahm, E., Heiber, I., Hoffmann, S. et al. Application of 23S rDNA-targeted oligonucleotide probes specific for enterococcito water hygiene control [J]. Appl. Microbiol. 1998,21,450- 453.
[91] Straub, J.A., Hertel, C, Hammes, W.P. A 23S rDNA-targeted polymerase chain reaction-based system for detection of Staphylococcus aureus in meat starter cuLtures and dairy products [J] . J. Food Prot. 1999, 62, 1150- 1156.
[92] Tesfaye, M., Holl, F.B. Group-specific differentiation of Rhizobium from clover species by PCR amplification of 23SrDNA sequences [J]. Can. J. Microbiol. 1998,44,1102- 1105.
[93] Hamid, M.E., Roth, A., Landt, O. et al. Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense species based on 16S-23S ribosomal DNA internal transcribed spacer sequences [J]. J. Clin. Microbiol. 2002,40, 707-711.
[94] Gu, X.X., Rossau, R., Jannes, G. et al. The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay [J]. Microbiol. 1998, 144, 1013-1019.
[95] Madico, G, Quinn, T.C., Boman, J. et al. Touchdown enzyme time release-PCR for detection and identification of Chlamydia trachomatis, C. pneumoniae, and C. psittaci using the 16S and 16S-23S spacer rRNA genes [J]. J. Clin. Microbiol. 2000,38,1085- 1093.
[96] Sasaki, Y., Yamamoto, K., Amimoto, K. et al. Amplification of the 16S-23S rDNA spacer region for rapid detection of Clostridiumchauvoei and Clostridium septicum [J]. Res. Vet. Sci. 2001, 71,227-229
[97]Brousseau,R.,Hill,J.E.,Prefontaine,G.et al.Streptococcus suis serotypes characterized by analysis ofchaperonin 60 gene sequences[J].Appl.Environ.Microbiol.2001,67,4828-4833.
[98]Kwok,A.Y.,Su,S.C.,Reynolds,R.P.et al.Species identification and hylogenetic relationships based on partial HSP60 gene sequences within the genus Staphylococcus[J].Int.J.Syst.Bacteriol.1999,49,1181-1192.
[99]Marston,E.L.,Summer,J.W.,Regnery,R.L.Evaluation of intraspecies genetic variation within the 60kDa heat-shock protein gene(groEL)of Bartonella species[J].Int.J.Syst.Bacteriol.,1999,49,1015-1023.
[100]Wang,R.F.,Beggs,M.L.,Robertson,L.H.et al.Design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples [J].FEMS Microbiol.Lett.2002,213,175-182.
[101]刘景武,张伟,何俊萍,周巍等,FTA滤膜用于PCR检测肉中的金黄色葡萄球菌研究[J].生物工程学报,2005.21(6),1009-1013