“肺与大肠相表里”理论的实验研究
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摘要
1研究目的
本文通过对“肺与大肠相表里”的理论及实验研究分析,旨在揭示其理论建构的合理性,探讨理论特色及客观性,以期为运用泻肺平喘灵治疗小儿痰热壅肺证哮喘提供理论依据。
2研究方法
2.1理论研究
采用中医理论发生学研究方法,对中医肺与大肠的基本概念、基本观点和基本理论的形成与演变,作出客观而确实的诠释；开展肺与大肠西医学相关性理论研究,以西医学中有关肺与大肠生理、病理联系的物质基础为依托,阐释肺系疾病与大肠疾病在发病机制、症状中的联系,探讨中医“肺与大肠相表里”的物质基础。
2.2实验研究
将70只SD大鼠随机等分为正常组、模型组、解扎组、三拗组、三通组、三活组及泻肺平喘灵组,每组各10只,选用体外直肠不全结扎造模,治疗组于术后72h从体外解除直肠结扎丝线并继续常规饲养,同时分别以三拗汤、三通方、三活方、泻肺平喘灵灌胃。
2.3检测指标的确定及检测方法
2.3.1一般状况观察：包括各组大鼠实验处置前后的外观、活动、摄食量、二便、体温、呼吸的频率和幅度、呼吸道分泌物、体重等。
2.3.2肺组织湿／干重比：处死动物后取右肺上叶准确称取湿重,干燥48h后,再称取干重,计算肺湿／干重比。
2.3.3肺、肠组织病理学变化观察：肺肠组织石蜡包埋切片,HE染色,用于光镜下观察。
2.3.4用酶联免疫吸附法(ELISA)测定血清中内毒素(ET)、肿瘤坏死因子a (TNF-α)的水平；以TBA法测定大鼠肺组织丙二醛含量；考马斯亮兰法(Bradford法)测定肺泡灌洗液中总蛋白含量；定磷法测定肺泡灌洗液中磷脂含量。
3结论
3.1传统理论“肺与大肠相表里”在临床具有重要指导意义。
3.2泻肺平喘灵通过降低模型动物血清中ET、TNF-α,降低肺组织中MDA,降低肺泡灌洗液中总蛋白,增加BALF中磷脂含量,从而保护受损的肺组织。
1. Objectives
Based on the theoretical analysis and experimental research of the theory of "The lung associated with the large intestine",the article aims at revealing the rationality of the construction, characteristics and objectivity theory, so as to put forward the evidence of exploiting Xiefei pingchuan ling to treat children phlegm-heat type asthma.
2. Methods
2.1 Research method of theories
The article adopts TCM genetic research method, explaining the formation and evolution of the basic conception,the basic ideas and the basic theory of TCM in lung and large intestine objectively and exactly. Studying the correlation between lung and large intestine according to the conception of these organs in western medicine,Based on the study of the physiological connection of lung and large intestine,to further investigate the relationship between lung diseases and genteropath of their pathogenesis and symptoms.And discuss the material foundation of the theory--"the Lung Associated with the Large Intestine" in traditional Chinese medicine.
2.2 Experimental researeh method
Seventy SD rats were equally divided into seven groups randomly:the normal group, the model group, the unhitch group,the Sanao group, the Santong group, the Sanhuo group and the Xiefei pingchuan ling group, Except those in the normal group were untreated, the others rats were established to intestinal obstruction model by incomplete ligation of the rectum in vitro.The ligation was relieved 72h after operation in the unhitch group and the treatment group, and the animals were fed continuously on routine. Meanwhile, Xiefei pingchuan ling and Its decomposed formulas were given.
2.3 Determination of Examination target and examination method
2.3.1 General condition observation:including big mice'outward appearance,the activity,food eating,,the feces and urine,the temperature,the breath frequency and scope,the respiratory tract secretion,the body weight of each group before and after the model-making.
2.3.2 Calculation of lung wet/dry weight ratios:After the animal was killed, choose the upper part of the right lung, weighs it,and then dry it for 48 hours, then weighs it again and calculate the wet/dry weight ratios of the lung.
2.3.3 Observation of the pathological change of the lung and large intestine organization:use paraffin section method to entrap the lung and large intestine organization, dye with HE method, and observe with optical microscope.
2.3.4 ELISA(enzyme linked immune-sorbent assay) method was used to measure the content of TNF-α,ET;the content of phospholipids and the total proteins(TP) in BALF was evaluated by the methods of determineate phosphorus content and Bradford respectively.
3. Conclusions
3.1 The theory of "the Lung Associated with the Large Intestine" plays an important role in the clinic treatment.
3.2 Xiefei pingchuan ling protect the damaged lung tissue of the model animals by decreasing the level of serum ET、TNF-α,MDA in the lung tissue,protein in the BALF,and increasing the level of TP in the BALF.
本文通过对“肺与大肠相表里”的理论及实验研究分析,旨在揭示其理论建构的合理性,探讨理论特色及客观性,以期为运用泻肺平喘灵治疗小儿痰热壅肺证哮喘提供理论依据。
2研究方法
2.1理论研究
采用中医理论发生学研究方法,对中医肺与大肠的基本概念、基本观点和基本理论的形成与演变,作出客观而确实的诠释；开展肺与大肠西医学相关性理论研究,以西医学中有关肺与大肠生理、病理联系的物质基础为依托,阐释肺系疾病与大肠疾病在发病机制、症状中的联系,探讨中医“肺与大肠相表里”的物质基础。
2.2实验研究
将70只SD大鼠随机等分为正常组、模型组、解扎组、三拗组、三通组、三活组及泻肺平喘灵组,每组各10只,选用体外直肠不全结扎造模,治疗组于术后72h从体外解除直肠结扎丝线并继续常规饲养,同时分别以三拗汤、三通方、三活方、泻肺平喘灵灌胃。
2.3检测指标的确定及检测方法
2.3.1一般状况观察：包括各组大鼠实验处置前后的外观、活动、摄食量、二便、体温、呼吸的频率和幅度、呼吸道分泌物、体重等。
2.3.2肺组织湿／干重比：处死动物后取右肺上叶准确称取湿重,干燥48h后,再称取干重,计算肺湿／干重比。
2.3.3肺、肠组织病理学变化观察：肺肠组织石蜡包埋切片,HE染色,用于光镜下观察。
2.3.4用酶联免疫吸附法(ELISA)测定血清中内毒素(ET)、肿瘤坏死因子a (TNF-α)的水平；以TBA法测定大鼠肺组织丙二醛含量；考马斯亮兰法(Bradford法)测定肺泡灌洗液中总蛋白含量；定磷法测定肺泡灌洗液中磷脂含量。
3结论
3.1传统理论“肺与大肠相表里”在临床具有重要指导意义。
3.2泻肺平喘灵通过降低模型动物血清中ET、TNF-α,降低肺组织中MDA,降低肺泡灌洗液中总蛋白,增加BALF中磷脂含量,从而保护受损的肺组织。
1. Objectives
Based on the theoretical analysis and experimental research of the theory of "The lung associated with the large intestine",the article aims at revealing the rationality of the construction, characteristics and objectivity theory, so as to put forward the evidence of exploiting Xiefei pingchuan ling to treat children phlegm-heat type asthma.
2. Methods
2.1 Research method of theories
The article adopts TCM genetic research method, explaining the formation and evolution of the basic conception,the basic ideas and the basic theory of TCM in lung and large intestine objectively and exactly. Studying the correlation between lung and large intestine according to the conception of these organs in western medicine,Based on the study of the physiological connection of lung and large intestine,to further investigate the relationship between lung diseases and genteropath of their pathogenesis and symptoms.And discuss the material foundation of the theory--"the Lung Associated with the Large Intestine" in traditional Chinese medicine.
2.2 Experimental researeh method
Seventy SD rats were equally divided into seven groups randomly:the normal group, the model group, the unhitch group,the Sanao group, the Santong group, the Sanhuo group and the Xiefei pingchuan ling group, Except those in the normal group were untreated, the others rats were established to intestinal obstruction model by incomplete ligation of the rectum in vitro.The ligation was relieved 72h after operation in the unhitch group and the treatment group, and the animals were fed continuously on routine. Meanwhile, Xiefei pingchuan ling and Its decomposed formulas were given.
2.3 Determination of Examination target and examination method
2.3.1 General condition observation:including big mice'outward appearance,the activity,food eating,,the feces and urine,the temperature,the breath frequency and scope,the respiratory tract secretion,the body weight of each group before and after the model-making.
2.3.2 Calculation of lung wet/dry weight ratios:After the animal was killed, choose the upper part of the right lung, weighs it,and then dry it for 48 hours, then weighs it again and calculate the wet/dry weight ratios of the lung.
2.3.3 Observation of the pathological change of the lung and large intestine organization:use paraffin section method to entrap the lung and large intestine organization, dye with HE method, and observe with optical microscope.
2.3.4 ELISA(enzyme linked immune-sorbent assay) method was used to measure the content of TNF-α,ET;the content of phospholipids and the total proteins(TP) in BALF was evaluated by the methods of determineate phosphorus content and Bradford respectively.
3. Conclusions
3.1 The theory of "the Lung Associated with the Large Intestine" plays an important role in the clinic treatment.
3.2 Xiefei pingchuan ling protect the damaged lung tissue of the model animals by decreasing the level of serum ET、TNF-α,MDA in the lung tissue,protein in the BALF,and increasing the level of TP in the BALF.
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