葫芦脲和药物与生物大分子相互作用及分析应用
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摘要
蛋白质和核酸是两类重要的生物大分子。蛋白质担负着各种生理功能，从整体上维持生物体新陈代谢活动的进行，是生物性状的直接表达者，而核酸是揭示生命遗传与变异的主要物质。因此，深入研究小分子物质与蛋白质和核酸的作用机理，建立蛋白质和核酸快速、简便的定量分析方法，对在分子水平上阐明生命的奥秘、开发新药和预防与治疗疑难病症等方面具有重要的意义，是当前生物分析化学研究的前沿和热点之一。
本论文以分析化学，生物化学为研究背景，利用荧光技术、共振光散射技术、吸收光谱技术、圆二色光谱技术、透射电镜技术和扫描电子显微镜技术等手段研究了葫芦脲、药物与蛋白质和核酸的相互作用机理。利用荧光、共振光散射技术建立了蛋白质和核酸快速、准确、简便、灵敏的定量分析方法。论文共分六个部分。
论文的第一部分评述了近年来蛋白质的荧光和共振光散射探针以及核酸的荧光探针的研究进展；并对新一代超分子主体化合物葫芦脲的分子识别作用以及利用荧光和吸收等光分析手段对葫芦脲与有机小分子相互作用的研究进行了综述。共引用文献188篇。
本论文的第二部分，研究了酸性条件下，葫芦脲对ANS-BSA的荧光增强效应，建立了灵敏的定量测定蛋白质的新方法，BSA的线性范围5.0×10~(-8)5.0×10~(-6)g／mL，检出限(S／N=3)为34ng／mL。将该方法用于人血清总蛋白的测定，结果令人满意。利用吸收光谱、园二色光谱、共振光散射光谱、荧光偏振、荧光寿命以及F(?)rster能量转移理论等对体系的作用机理进行了研究。研究表明，CB使得BSA的疏水微区暴露出来，更易于与ANS结合，从而促进了BSA吸收的能量向ANS转移，导致其荧光寿命的增加和量子产率的提高；CB和ANS的协同作用能导致BSA有强的去折叠作用。基于上述研究，提出了CB-ANS-BSA夹心式结构模型，即ANS结合在BSA的疏水微区后，再以分子间作用力结合CB，形成了CB-ANS-BSA夹心式结构的聚集体。
论文第三部分合成了新的纳米微粒葫芦脲银，并将其用于蛋白质的定量测定。利用柠檬酸钠还原法制备了银纳米粒子(nanoAg)，并合成了新的纳
Proteins and nucleic acids are very important bio-macromolecules. Different proteins perform a wide variety of biological functions. Some proteins are enzymes, which catalyze chemical reactions. Other proteins play structural or mechanical roles, such as maintenance the metabolism of plants and animals directly express biologic characters. Nucleic acids play a central role in the storage replication of hereditary and aberrance information and in the expression of this information through synthesis. In order to surmount many difficult diseases and explore the secret of life, we further investigate the interaction between small molecules and nucleic acids or proteins. A rapid and convenient method is discovered for quantitatively analyzing nucleic acids and proteins.
Based on the research of analytical chemistry and biochemistry, this dissertation investigated the interaction mechanism between small molecules, supermolecules or nanoparticles and proteins as well as small molecules and nucleic acids using the multi-techniques including fluorescence, resonance light scattering, absorption, Circular Dichroism, Transmission Electron Microscope and Scanning Electron Microscope. Several rapid, efficient methods, which are of high sensitivity, are developed for determining proteins and nucleic acids. This dissertation is written in six chapters.
The progress of fluorescence probes and resonance light scattering probes for proteins is summarized. The progress of fluorescence probes for nucleic acids is recapitulated. The new macromolecule host compound cucurbituril (CB) and the interaction between CB and metal ions or small organic molecules were reviewed in the first chapter. References (188) are cited here.
Second chapter describes the properties and the scope of application of CB. It is found that CB can enhance the fluorescence of 1,8-ANS-BSA system in
本论文以分析化学，生物化学为研究背景，利用荧光技术、共振光散射技术、吸收光谱技术、圆二色光谱技术、透射电镜技术和扫描电子显微镜技术等手段研究了葫芦脲、药物与蛋白质和核酸的相互作用机理。利用荧光、共振光散射技术建立了蛋白质和核酸快速、准确、简便、灵敏的定量分析方法。论文共分六个部分。
论文的第一部分评述了近年来蛋白质的荧光和共振光散射探针以及核酸的荧光探针的研究进展；并对新一代超分子主体化合物葫芦脲的分子识别作用以及利用荧光和吸收等光分析手段对葫芦脲与有机小分子相互作用的研究进行了综述。共引用文献188篇。
本论文的第二部分，研究了酸性条件下，葫芦脲对ANS-BSA的荧光增强效应，建立了灵敏的定量测定蛋白质的新方法，BSA的线性范围5.0×10~(-8)5.0×10~(-6)g／mL，检出限(S／N=3)为34ng／mL。将该方法用于人血清总蛋白的测定，结果令人满意。利用吸收光谱、园二色光谱、共振光散射光谱、荧光偏振、荧光寿命以及F(?)rster能量转移理论等对体系的作用机理进行了研究。研究表明，CB使得BSA的疏水微区暴露出来，更易于与ANS结合，从而促进了BSA吸收的能量向ANS转移，导致其荧光寿命的增加和量子产率的提高；CB和ANS的协同作用能导致BSA有强的去折叠作用。基于上述研究，提出了CB-ANS-BSA夹心式结构模型，即ANS结合在BSA的疏水微区后，再以分子间作用力结合CB，形成了CB-ANS-BSA夹心式结构的聚集体。
论文第三部分合成了新的纳米微粒葫芦脲银，并将其用于蛋白质的定量测定。利用柠檬酸钠还原法制备了银纳米粒子(nanoAg)，并合成了新的纳
Proteins and nucleic acids are very important bio-macromolecules. Different proteins perform a wide variety of biological functions. Some proteins are enzymes, which catalyze chemical reactions. Other proteins play structural or mechanical roles, such as maintenance the metabolism of plants and animals directly express biologic characters. Nucleic acids play a central role in the storage replication of hereditary and aberrance information and in the expression of this information through synthesis. In order to surmount many difficult diseases and explore the secret of life, we further investigate the interaction between small molecules and nucleic acids or proteins. A rapid and convenient method is discovered for quantitatively analyzing nucleic acids and proteins.
Based on the research of analytical chemistry and biochemistry, this dissertation investigated the interaction mechanism between small molecules, supermolecules or nanoparticles and proteins as well as small molecules and nucleic acids using the multi-techniques including fluorescence, resonance light scattering, absorption, Circular Dichroism, Transmission Electron Microscope and Scanning Electron Microscope. Several rapid, efficient methods, which are of high sensitivity, are developed for determining proteins and nucleic acids. This dissertation is written in six chapters.
The progress of fluorescence probes and resonance light scattering probes for proteins is summarized. The progress of fluorescence probes for nucleic acids is recapitulated. The new macromolecule host compound cucurbituril (CB) and the interaction between CB and metal ions or small organic molecules were reviewed in the first chapter. References (188) are cited here.
Second chapter describes the properties and the scope of application of CB. It is found that CB can enhance the fluorescence of 1,8-ANS-BSA system in
引文
[1] Boyd K. Hartman and Sidney UdenfriendA method for immediate visualization of proteins in acrylamide gels and its use for preparation of antibodies to enzymes[J]. Analytical Biochemistry. 1969,30(3): 391-394
[2] Ohning GV, Neet KE. 6-(p-toluidinyl)naphthalene-2-sulfonic acid as a fluorescent probe of yeast hexokinase: conformational states induced by sugar and nucleotide ligands[J]. Biochemistry. 1983,22(12):2986-2995.
[3] R. F. Steiner, H. Edelhoch, The Properties of Thyroglobulin. Ⅵ. The Internal Rigidity of Native and Denatured Thyroglobulin [J]. J. Am. Chem. Soc., 1961, 83(6): 1435-1444.
[4] G. R. Shephenol, B. J. Noland. A fluorescence assay suitable for histone solutions [J]. Anal. Biochem., 1965, 11(3): 443-448.
[5] T.H. Steinberg, R.P. Haugland, V. Singer. Applications of sypro orange and sypro red protein gel stains [J]. Anal. Biochem., 1996, 239(2): 238-245.
[6] 刘保生，高静，杨更亮．吖啶橙罗丹明6G荧光共振能量转移及其罗丹明6G荧光猝灭法测定蛋白质[J]．分析化学，2005，33(4)：546-548
[7] 朱英贵，高峰，章丽，谢筱娟，王伦．新荧光试剂1-甲酰基苯并三唑缩邻氨基苯酚的合成及其在蛋白质测定中的应用[J]．分析化学，2005，33(5)：157
[8] Cui FL, Fan J, Li JP, Hu ZD Interactions between 1-benzoyl-4-pchlorophenyl thiosemicarbazide and serum albumin: investigation by fluorescence spectroscopy [J]. Bioorganic & Medicinal Chemistry. 2004, 12 (1): 151-157
[9] Chen Y, Yao MN, Tang Y J, Li YQ. Determination of protein with TPPS by total internal reflection synchronous fluorescence spectroscopy[J]. Spectroscopy and Spectral Analysis 2005, 25 (12): 2048-2051
[10] A. Sukowska, J. Rownicka, B. Bojko and W. Sukowski. Interaction of anticancer drugs with human and bovine serum albumin[J]. Journal of Molecular Structure 2003, 651-653:133-140
[11] Anna Sukowska. Interaction of drugs with bovine and human serum albumin[J].Journal of Molecular Structure, 2002,614(1-3): 227-232
[12] Wen-wu Qina, Guo-quan Gonga and Yu-min Song. Determination of proteins by fluorescence quenching of Magdala Red[J]. Spectrochimica Aeta Part A: 2000,56(5): 1021-1025
[13] Claire Dufour, Olivier Dangles. Flavonoid-serum albumin complexation: determination of binding constants and binding sites by fluorescence spectroscopy[J]. Biochimica et Biophysica Acta, 2005, 1721(1-3): 164-173
[14] Y.J. Luo, H.X. Shen. Study on Acridine Orange dimer as a new fluorescent probe for the determination of protein [J]. Anal. Commun., 1999, 36(4): 135-138.
[15] 陈鸿琪，夏闽．荧光染料吖啶红作测定蛋白质的生物探针[J]．理化检验一化学分册，2001，37(2)：53-55
[16] 高峰，朱昌青，汪乐余，王伦．灿烂甲酚蓝与表面活性剂的作用及其在蛋白质测定中的应用[J]．分析化学 2002，30(3)：324-326
[17] J. C. Garcia-Borron, J. Escribano, M. Jimenez and J. L. Iborra. Quantitative determination of tryptophanyl and tyrosyl residues of proteins by second-derivative fluorescence spectroscopy[J]. Analytical Biochemistry, 1982, 125 (2):277-285
[18] 高峰，朱昌青，王伦．耐尔蓝-十二烷基苯磺酸钠-蛋白质体系的荧光反应及其分析应用[J]．分析化学 2003，3 1(9)：1085-1088
[19] J. B. Joseph. Fluorometric microdetermination of human serum albumin [J]. Anal. Chem., 1960, 32(4): 560-563.
[20] Y.X. Ci, L. Chen. Fluorimetric determination of human serumal bumin with eriochromecyanine [J]. Analyst, 1988, 113 (4): 679-681.
[21] S. Takaji, O. Tatsuya, I. Kayo, K. Kazumi, M. Naomi, C. Massahiko, S. Yutaka, K. Massao, N. Sonko, I. Takateru. Determination of trace amounts of albumin in human bronchoalveolar lavage fluids by fluorometry with chromazurol S [J]. Clin. Chem. Acta, 1994, 229(1-2): 37-47.
[22] 张明翠，汪乐余，李玲，陈红旗，张德兴，王伦．新荧光试剂5-(4-羧基苯偶氮)-8-水杨醛缩氨基喹啉荧光增敏法测定人血清蛋白质[J]．分析化学，2004，32(3)：74-76
[23] T. Kinoshita, J. Murayama, A. Tsuji. Fluorophotometric assay of pr eins using hypochlorite-thiamine reagent [J]. Chem. Pharm. Bull., 1976, 24(11): 2901-2902.
[24] T. Kinoshita, J. Murayama, K. Murayama, A. Tsuji. Microanalysis of proteins and peptides novel method for the fluorometric assay of proteins using hypochlorite-thiamine reagent [J]. Chem. Pharm. Bull., 1980, 28(2): 641-644.
[25] C.Q. Ma, K.A. Li, S.Y. Tong. Determination of proteins by fluorescence quenching of erythrosin B [J]. Anal. Chim. Acta, 1996, 333(1-2): 83-88.
[26] T.H. Steinberg, R.P. Haugland, V. Singer. Applications of sypro orange and sypro red protein gel stains [J]. Anal. Biochem., 1996, 239(2): 238-245.
[27] N. Li, K.A. Li, S.Y. Tong. Fluorometric determination for micro amounts of albumin and globulin fractions without separation by using α, β, γ, δ,-tetra(4'-carboxyphenyl) porphin [J]. Anal. Biochem., 1996, 233 (2): 151-155.
[28] H. Ichiba, H. Niwayama, T. Yajima. High-sensitive flow-injection analysis of proteins coupled with online alkaline-hydrolysis and the fluorescent derivatization method [J]. Chem. Pharm. Bull., 1989, 37(11): 3155-3157.
[29] L.B. Maria. Protein assay in the microgram range: Modifications of the hiraoka-glick method [J]. Anal. Biochem., 1973, 53(1): 12-20.
[30] 李东辉，杨黄浩，郑洪，陈宾，陈秋影，许金钩．用四磺基铝酞菁直接测定血清中自蛋白[J]．分析化学，1999，27(9)：1018-1021．
[31] D.H. Li, H.H. Yang, H. Zhen, Y. Fang, Q.Z. Zhu, J.G. Xu. Fluorimetric determination of albumin and globulin in human serum using tetra-substituted sulphonated aluminum phthalocyanine [J]. Anal. Chim. Acta, 1999, 401(1-2): 185-189.
[32] M. J. Baar, G. Patonay. Ultrasensitive detection of closely related angiotensin i peptides using capillary electrophoresis with near-infrared laser-induced fluorescence detection [J]. Anal. Chem., 1999, 71 (3): 667-671.
[33] B. L. Legender Jr., D.L. Moberg, D.C. Williams, S.A. Soper. Ultrasensitive near-infrared laser-induced fluorescence detection in capillary electrophoresis using a diode laser and avalanche photodiode [J]. J. Chromatogr. A, 1997, 779 (1-2): 185-194.
[34] A. Chopdar, A.M. Turk, D.W. Hill. Fluorescent infra-red angiography of the fundusoculi using indocyanine green dye [J]. Trans. Ophthal. Soc., 1978, 98:142-1146.
[35] D. A. Wilberforce, G. Patonay. Investigation of near-infrared laser dye albumin complexes [J]. Spectrochim. Acta Part A, 1990, 46(8): 1153-1162.
[36] 郑洪，李江辉．合成的近红外花菁染料测定痕量血清蛋白质[J]．高等学校化学学报，1999，20(4)：555-557．
[37] M.A. Kessler, O.S. Wolfbeis. Eur. Pat. Appl., 1991, 20: 678.
[38] E. Terpetsching, H. Szmacinski, J.R. Lakowicz. Synthesis, spectral properties and photostabilities of symmetrical and unsymmetrical squarines; a new class of flourophores with long-wavelength exiciation and emission [J]..Anal. Chim. Acta, 1995, 282(3): 633-641.
[39] E. Terpetschnig, H. Szmacinski, A. Ozinskas, J.R. Lakowicz. Synthesis of squaraine-n-hydroxysuccinimide esters and their biological application as long-wavelength fluorescent labels [J]. Anal. Biochem., 1994, 217(2): 197-204.
[40] T. Imasaka, T. Fuchigami, N. Ishibashi. Micellar Electrokinetic capillary chromatography of porphinato chelates as a spectrophotometric approach to sub-femtomole detection of metal chelates [J]. Anal. Sci., 1991, 7(3): 491-498.
[41] T. Higashijima, T. Fuchigami, T. Imasaka, N. Ishibashi. Determination of amino acids by capillary zone electrophoresis based on semiconductor laser fluorescence detection [J]. Anal. Chem., 1992, 64(7): 711-714.
[42] A. J. G. Mank, H. T. C Vander Laan, H. Lingeman, C. Gooijer, U.A.Th. Brinkman, N.H. Velthorst. Visible diode laser-induced fluorescence detection in liquid chromatography after precolumn derivatization of amines [J]. Anal. Chem., 1995, 67(10): 1742-1748.
[43] M. B. Brown, T. E. Edmonds, J.N. Miller, D.P. Riley, N.J. Scare. Novel instrumentation and biomedical applications of very near infrared fluorescence [J]. Analyst, 1993, 118(4): 407-410.
[44] Zhu CQ, Wu YQ, Zheng H, Chen JL, Li DH, Li SH, Xu JG.. Near-infrared fluorometric determination of protein by shifting the ion-association equilibrium between cationic heptamethylene cyanine and poly-glutamate[J]. Analytical Letters, 2004, 37 (6): 1115-1127
[45] Patonay G, Kim JS, Kodagahally R, Strekowski L. Spectroscopic study of a novel bis(heptamethine cyanine) dye and its interaction with human serum albumin[J]. Applied Spectroscopy, 2005, 59 (5): 682-690
[46] 王守业，余华明，张祖德，刘清亮．荧光探针在蛋白质研究中的应用[J]．大学化学，1998，13(3)：5-10
[47] Breen P J, Hild EK, Horrocks WD Jr. Spectroscopic studies of metal ion binding to a tryptophan-containing parvalbumin[J]. Biochemistry, Biochemistry. 1985, 24(19): 4991-4997.
[48] 刘清亮，徐晓龙，王淳，谢波平，余华明．稀土及过渡金属离子对蛇毒内抗凝血因子荧光光谱的影响[J]．中国稀土学报，1992，10(2)：106-109
[49] J. H. Yang, G.J. Zhou, G.L. Zhang, Z.K. Si, J.T. Hu. Determination of some cephalosporins in pharmaceutical formulations by a fluorescence quenching method [J]. Anal. Commun., 1996, 33(5): 167-170.
[50] Changxia Sun, Jinghe Yang, Xia Wu, The fluorescent enhancement effect in terbium-gadolinium-protein-cetylpyridine bromide system and its application for the determination of protein at nanogram level[J]. Biochimie, 2004, 86(8): 569-578
[51] Changxia Sun, Jinghe Yang, Xia Wu,Benyu Su,Shufang Liu ,The fluorescent enhancement effect in terbium-gadolinium-protein- cetylpyridine bromide system and its application for the determination of protein at nanogram level[J]. Chem. Phys. Lett;2004,398 (4-6):343-350
[52] 黄淑萍，陈亮，李玉红．比较与研究金属离子与三种蛋白的配位机理[J]．光谱学与光谱分析，1995，15(5)：85-89
[53] X. D. Yang, Y.X. Ci, W.B. Chang. Time-resolved fluorescence immunoassay with measurement of a europium chelate in solution: dissociation conditions and application for determination of cortisol [J]. Anal. Chem., 1994, 66 (15): 2590-2594.
[54] Y.X. Ci, W.B. Wang, X.D. Yang. Fluorescence labelling with europium chelate of β-diketones and application in time-resolved fluoroimmunoassays (TR-FIA) [J]. Journal of Immunological Methods, 1995, 179(2): 233-241
[55] X. D. Yang, Y.X. Ci, W.B. Chang. Time-resolved fluorescence immunoassay with measurement of a europium chelate in solution: dissociation conditions and application for determination of cortisol [J]. Anal. Chem., 1994, 66 (15): 2590-2594.
[56] Y.X. Ci, W.B. Wang, X.D. Yang. Fluorescence labelling with europium chelate of β-diketones and application in time-resolved fluoroimmunoassays (TR-FIA) [J]. Journal of Immunological Methods, 1995, 179(2): 233-241
[57] ChangyingGuo, Xia Wu, Jingheyang, Fei Wang, Zhen Jia, Dehuan Ran, Jinhua Zheng. Determination of proteins using fluorescence enhancement of Tb-benzoylacetone-sodium dodecyl benzene sulfonate- protein system[J]. J. photochem. Photobio. A 2005 Nov. 29 online
[58] 史华红，杨燕生，龚孟濂，陈泮森．铽标记人血清白蛋白的荧光光谱及应用，发光学报，1996，17(9)：240-244
[59] A. Canfi, M. P. Bailey, B. F. Rocks. Multiple labeling of immunoglobulin G, Albumin and testosterone with a fluorescent Terbium chelate for fluorenscence immunoassays [J]. Analyst, 1989, 114(11): 1407-1411.
[60] R.A. Evangelista, A. Pallak, B. Allore, E.F. Yernplaton, R.C. Motin, E.P. Diamandis. A new europium chelate for protein labelling and time-resolved fluorometric applications [J]. Clin. Biochem., 1988, 21(3): 173-178.
[61] E. Reichstein, Y. Shami, M. Ramjeesingh, E. P. Diamandis. Laser-excited time-resolved solid-phase fluoroimmunoassays with the new europium chelate 7-bis(chlorosulfophenyl)- 1,10-phenanthroline -2,9-dicarboxylic acid as label[J]. Anal. Chem., 1988,60(10): 1069-1074.
[62] Shufang Liu, Jinghe Yang, Xia Wu, Fluorometric determination of proteins using the yttrium(Ⅲ)-Sodium Lauryl Sulfate-Rutin-protein system[J]. J.Luminescence, 2006, 117(2):217-224.
[63] Jia Z, Yang JH, Wu X, et al. Study on a fluorometric method for the determination of protein in serum using quercetin-lanthanum (Ⅲ)-sodium dodecyl benzene sulfonate-protein system[J]. Anal. Lett. 39 (1): 67-81 2006
[64] 高建华，翟海云，陈彬．杯[8]芳烃与铈D形成的镧系超分子作荧光探针测定蛋白质[J]．分析化学，2002，30(3)：295-297
[65] Fei Wang, Jinghe Yang,Xia Wu, Fluorescence Enhancement effect for the determination of proteins with Morin-Al3+- Cetyltrimethylammonium Bromide[J], Talanta,2005, 67(4):836-842
[66] 杨美玲，杨培菊，宋玉民．芦丁金属配合物的合成、表征及与血清白蛋白的相互作用[J]．无机化学学报，2005，21(4)483-489
[67] M. B. Brown Jr., M. Moronne, P. Gin, S. Weiss, A.P. Alivisatos. Semiconductor nanocrystals as fluorescent biological labels [J]. Science, 1998, 281: 2013-2016.
[68] Marcel Bruchez Jr., Mario Moronne, Peter Gin, Shimon Weiss, A. Paul Alivisatos. Semiconductor Nanocrystals as Fluorescent Biological Labels[J]. Science,1998, 281 (5385) : 2013-2016.
[69] 徐力，郭轶，解仁国，庄家骐，王连英，杨文胜，李铁津·水相合成CdS纳米晶标记牛血清白蛋白[J]．功能材料与器件学报2003，9(2)：201-204
[70] Zhu CQ, Zhao DH, Chen JL, Li YX, Wang LY, Wang L, Zhou YY,Zhuo S J, Wu YQ. Application of L-cysteine-capped nano-ZnS as a fluorescence probe for the determination of proteins[J]. Analytical and Bioanalytical Chemistry, 2004, 378 (3): 811-815
[71] Wang L, Chen HQ, Wang LY, Li L, Xu FG, Liu JS, Zhu CQ. Preparation and application of a novel composite nanoparticle as a protein fluorescence probe[J]. Analytical Letters, 2004, 37 (2): 213-223
[72] Wang LY, Kan XW, Zhang MC, Zhu CQ, Wang L. Fluorescence for the determination of protein with functionalized nano-ZnS[J]. Analyst, 2002, 127(11): 1531-1534
[73] E.A. Medcalf, D.J. Newman, E.G. Gorman, C.P. Proce. Rapid, robust method for measuring low concentrations of albumin in urine [J]. Clin. Chem., 1990, 36(3): 446-449.
[74] J.R. Taylor, M.M. Fang, S. Nie. Probing specific sequences on single DNA molecules with bioconjugated fluorescent nanoparticles [J]. Anal. Chem., 2000, 72(9): 1979-1986.
[75] Wang LY, Wang L, Dong L, Bian GR, Xia TT, Chen HQ. Direct fluorimetric determination of gamma-globulin in human serum with organic nano- particle biosensor [J]. Spectrochimica acta Part A, 2005, 61 (1-2): 129-133
[76] 敖丽梅，高峰，潘碧峰，崔大祥，古宏晨．研究金纳米粒子和牛血清白蛋白以及羊抗兔免疫球蛋白G的相互作用[J]．Chinese Journal of Chemistry，2006，(2)：253-256
[77] Evgenia Matveeva, Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski and Joseph R. Lakowicz. Metal-enhanced fluorescence immunoassays using total internal reflection and silver island-coated surfaces [J]. Analytical Biochemistry, 2004, 334(2): 303-311.
[78] Beverloo HB, van Schadewijk A, Bonnet J, van der Geest R, Runia R, Verwoerd NP, Vrolijk J, Ploem JS, Tanke HJ. Preparation and microscopic visualization of multicolor luminescent immunophosphors. Cytometry[J]. 1992, 13(6): 561-70.
[79] T. Toyooka, M. Ishibashi, T. Terao. Sensitive determination of n-terminal prolyl peptides by high-performance liquid-chromatography with laser-induced fluorescence detection [J]. J. Chromatogr. A, 1994, 661(1-2): 105-112.
[80] D.S. Stegehuis, U.R. Tjaden, C.M.B. Van den Beld, J. Van der Greef. Bioanalysis of the neuropeptide des-enkephalin-γ-endorphin by high-performance liquid chromatography with on-line sample pretreatment using gel permeation and solid-phase isolation [J]. J. Chromatogr. A, 1991, 549:185-193.
[81] V.H. Cohn, J.Lyle, A fluorometric assay for glutathione [J]. Anal. biochem., 1966, 14(3): 434-440.
[82] R. L. White, A. C. DeMarco, S. Shapiro, L. C. Vining, S. Wolfe. Measurement of 8-(L-a-aminoadipyl)-L -cysteinyl-D-valine synthetase activity in Streptomyces clavuligerus by high-performance liquid chromatography after precolumn derivatization with o-phthaldialdehyde [J]. Anal. Biochem., 1989, 178(2): 399-403.
[83] G. Szokan. Precolumn derivatization in hplc of amino-acids and peptides [J]. J. Liq. Chromatogr., 1982, 5(8): 1493-1498.
[84] H. Koning, H. Wolf, K. Venema, J. Korf. Automated procolumn derivatization of amino acids, small peptides, brain amines and drugs with primary amino groups for reversed-phased high-performance liquid chromatography using naphthalenedialdehyde as the fluorogenic label [J]. J. Chromatogr., 1990, 533: 171-178.
[85] D.M. Pinto, E.A. Arriaga, D. Craig, J. Angelova, N. Sharma, H. Ahmadzadeh, N.J. Dovichi, C.A. Boulet. Picomolar assay of native proteins by capillary electrophoresis precolumn labeling, submicellar separation, and laser-induced fluorescence detection [J]. Anal. Chem., 1997, 69(15): 3015-3021.
[86] J.P. Liu, Y.Z. Hsieh, D. Wiesler, M. Novotny. Design of 3-(4-carboxybenzoyl) -2- quinolinecarboxaldehyde as a reagent for ultrasensitive determination of primary amines by capillary electrophoresis using laser fluorescence detection [J]. Anal. Chem., 1991, 63(5): 408-412.
[87] A.F. Lehninger. Biochemie 2 [M].Auflage: Verlag Chem Weinheim, 1979.
[88] A.M. Felix, M.H. Jimenez. Rapid fluorometric detection for completeness in solid phase coupling reactions [J]. Anal. Biochem., 1973, 52(2): 377-381.
[89] Y. Hiraga, T. Kinoshita. Post-column derivatization of guanidino compounds in high-performance liquid-chromatography using ninhydrin [J]. J. Chromatogr., 1981, 226(1): 43-51.
[90] G.R. Rhodes, V.K. Boppana. High-performance liquid- chromatographic analysis of arginine-containing peptides in biological-fluids by means of a selective post-column reaction with fluorescence detection [J]. J. Chromatogr., 1988,444: 123-131.
[91] C.M.B. van den Beld, U.R. Tjaden, N.J. Reinhoud, D.S. Stegehuis, J. van der Greef. Determination of some P-endorphin fragments in human plasma by high-performance liquid chromatography with laser induced fluorescence detection [J]. J. Control. Release, 1993, 13: 129-139.
[92] V. Fierabracci, P. Masiello, M Novelli. Application of amino-acid-analysis by high-performance liquid- chromatography with phenyl isothiocyanate derivatization to the rapid-determination of free amino-acids in biological samples [J]. J. Chromatogr., 1991, 570(2): 285-291.
[93] S.A. Cohen, D.P. Michaud. Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography [J]. Anal. Biochem., 1993, 211 (2): 279-287.
[94] M. Albin, R. Weinberger, E. Sapp, S. Moring. Fluorescence detection in capillary electrophoresis: evaluation of derivatizing reagents and techniques [J]. Anal. Chem., 1991, 63(5): 417-422.
[95] H. Bruckner, M. Lupke. Use of chromogenic and fluorescent oxycarbonyl chlorides as reagents for amino-acid-analysis by high-performance liquid-chromatography [J]. J. Chromatogr. A, 1995, 697(1-2): 295-307.
[96] V. Fierabracci, P. Masiello, M. Novelli, E. Bergamini. Application of amino acid analysis by high-performance liquid chromatography with phenyl isothiocyanate derivatization to the rapid determination of free amino acids in biological samples [J]. J. Chromatogr. B., 1991, 570 (2): 285-291.
[97] M.A. Cichy, D.L. Stegmeier, H. Veening, H.D. Becker. High-performance liquid chromatographic separation of biogenic polyamines using 2-(1-pyrenyl)ethyl chloroformate as a new fluorogenic derivatizing reagent [J]. J. Chromatogr. B. 1993, 613 (1): 15-21.
[98] A.J. Faulkner, H. Veening, H.D. Becker, 2-(9-Anthryl)ethyl chloroformate: a precolumn derivatizing reagent for polyamines determined by liquid chromatography and fluorescence detection [J]. Anal. Chem., 1991, 63(3): 292-296.
[99] Huang C.Z., Li K.A., Tong S.Y., Determination of Nanograms of Nucleic Acids by Their Enhancement Effect on the Resonance Light Scattering of the Cobalt(Ⅱ)/4-[(5-Chloro-2-pyridyl)azo]- 1,3-diaminobenzene Complex[J]. Anal. Chem.,1997,69(3): 514--520
[100] Huang C.Z., Zhu J.X., Li K.A., Tong S.Y., Determination of Albumin and Globulin at Nanogram Levels by a Resonance Light-Scattering Technique with α,β,γ,δ-Tetrakis(4-sulfophenyl)porphine[J]. Anal. Sci., 1997, 13(2): 263-268
[101] Anglister J, Steinberg I. Z. Depolarized Rayleigh light scattering in absorption bands measured in cycopene solution[J]. Chem. Phys. Lett., 1979,65(1):50-54
[102] 王亚婷，赵凤林，李克安，童沈阳 有机染料作为共振光散射探针在生物分析中的研究进展[J]．高等学校化学学报，2000，21(10)：1491-1497
[103] 王甫丽，郝英霞，张红蕾，赵勇，刘保生，张红医．苯酚磺酞类酸性染料与蛋白质包合物的共振散射光谱[J]．河北大学学报(自然科学版)：2002，22(1)：32-35
[104] C.Z. Huang, Y.F. Li, J.G. Mao, D.G. Tan. Determination of protein concentration by enhancement of the preresonance light-scattering of α,β,γ,δ-tetrakis (5-sulfothienyl) porphine[J]. Analyst, 1998, 123(6): 1401-1406.
[105] C. Q. Ma, K.A. Li, S.Y. Tong. Microdetermination of proteins by resonance light scattering spectroscopy with bromophenol blue [J]. Anal. Biochem., 1996, 239(1): 86-91.
[106] Z. G.Chen, T.Y. Zhang, F.L. Ren, W.F. Ding. Determination of Proteins at Nanogram Levels Based on Their Resonance Light Scattering Decrease Effect on the Dibromo-o-Nitrophenylfluorone-Sodium Lauroyl Glutamate Systema[J]. Microchim Acta, 2006,153 (1-2): 65-71
[107] 李树伟，李娜，赵凤林，李克安．用铀试剂Ⅰ共振光散射法测定蛋白质的研究[J]．光谱学与光谱分析，2002，22(4)：619-622
[108] 刘保生，张红医，张红蕾，赵勇．氯酚红-蛋白体系的共振光散射法定量测定蛋白质[J]．2003，23(2)：229-231．
[109] 杨传孝，李原芳，黄承志．，丽春红G用于人血清样品中总蛋白的共振光散射测定[J]．分析化学，2003，31(2)，148-152．
[110] Li yuanfang,Huang Chengzhi,Li Ming. A Resonance Light Scattering Determination of Proteins with Fast Green FCF[J]. Anal. Sci., 2002, 18, 177-181.
[111] Rutao Liu, Jinghe Yang, Xia Wu, Zengjun Lan, Resonance double light scattering method for the determination of proteins with morin/CTMAB [J]. Spectrochimica Acta Part A, 2002, 58(14):3077-3083
[112] Y.J. Chen, J.H. Yang, X. Wu, T. Wu, Y.X. Luan. Resonance light scattering technique for the determination of proteins with resorcinol yellow and OP [J]. Talanta, 2002, 58(5): 869-874.
[113] Xia Wu, Shuna Sun, Changying Guo, Jinghe Yang, Changxia Sun, Chengrong Zhou, Tao Wu. Resonance light scattering technique for the determination of proteins with Congo red and Triton X-100 [J]. Luminescence, 2006,21(1): 56-61
[114] Suling Feng, Zihong Pan and Jing Fan Determination of trace proteins with pyronine Y and SDS by resonance light scattering [J]. Analytical and Bioanalytical Chemistry, 2005,383(2):255-260
[115] Rutao Liu, Jinghe Yang, Changxia Sun, Xia Wu, Lei Li, Zhengmin Li, Resonance Light Scattering Method for the Determination of BSA and HSA with Sodium Docecyl Benzene Sulfonate or Sodium Laury Sulfate[J]. Anal, Bioanal. Chem., 2003,377 (2):375-379
[116] Lun Wang,Yongxin Li , Determination of proteins based on their resonance light scattering enhancement effect on manganese-tetra-sulfonatophthalocyanine[J]. Microchimica Acta ,2003, 143(4):275-279
[117] R.P. Jia, L.J. Dong, Q.F. Li, X.G. Chen, Z.D. Hu, Y. Nagaosa, Study of the interaction of protein and dibromomethyl-Arsenazo-Al(Ⅲ) by Rayleigh light-scattering techniqu[J]. Anal. Chim. Acta, 2001,442 (2): 249-256.
[118] L. J. Dong, Run Ping Jia, Qian Feng Li, X. G. Chen, Zhi De Hu, M. A. Hooper. Microdetermination of proteins with the arsenazo-DBN-Al(Ⅲ) complex by Rayleigh light-scattering technique and application of the method[J]. Fresenius' Journal of Analytical Chemistry 2001,370(8):1009- 1014
[119] Run-Ping Jia, Li-Jun Dong, Qian-Feng Li, Xing-Guo Chen and Zhi-De Hu. A highly sensitive assay for protein with dibromochloro -arsenazo-A13+ using resonance light scattering technique and its application[J]. Talanta, 2002,57(4): 693-700
[120] 许发功，王伦，汪乐余，陈红旗，张莉，陶海升．ZnS-Zn2+纳米溶胶作为共振光散射探针检测γ2G球蛋白[J]．分析科学学报，2005，21(3)：265-267
[121] 许发功，王伦，汪乐余，陈红旗，张莉，刘金水．利用CuS／Cu2+纳米溶胶作为共振光散射探针检测γ-球蛋白[J]．光谱学与光谱分析，2005，25(7)：1095-1098
[122] Le-Yu Wang, Lun Wang, Ling Dong, Yan-Li Hu, Ting-Ting Xia, Hong-Qi Chen, Ling Li and Chang-Qing Zhu. Determination of γ-globulin at nanogram levels by its enhancement effect on the resonance light scattering of functionalized HgS nanoparticles[J]. Talanta, 2004,62(2):237-240
[123] 张萍，卓淑娟，陈金龙，汪乐余，朱昌青．藉L-半胱氨酸包覆的ZnS纳米粒子的共振光散射定量分析蛋白质分析试验室[J]．2005，24(3)：86-90
[124] 梁爱惠，邹节明，蒋治良，王力生，覃爱苗．磷钼杂多酸与蛋白质相互作用的共振散射光谱研究[J]．应用化学，2002，19(8)：768-771
[125] 夏传俊，张莉，张锐，赵广超．纳米银胶与蛋白质的相互作用及应用[J]．安徽师范大学学报(自然科学版)，2003，26(2)：146-148
[126] Zheng Ping Li, Xin Rui Duan, Cheng Hui Liu and Bao An Du. Selective determination of cysteine by resonance light scattering technique based on self-assembly of gold nanoparticles[J]. Analytical Biochemistry, 2006, 351(1): 18-25
[127] Zhenxin Wang, Jason Lee, Andrew R. Cossins, and Mathias Brust. Microarray-Based Detection of Protein Binding and Functionality by Gold Nanoparticle Probes[J]. Anal. Chem., 2005,77(17):5770-5774
[128] M. J. Waring, Complex formation between ethidium bromide and nucleic acids[J]. J. Mol. Biol., 1965, 13(1): 269-282
[129] 何品刚，孙星炎，徐春，刘盛辉，陈奕利，方禹之．脱氧核糖核酸与双苯甲亚胺相互作用的荧光特性研究[J]．分析化学，1999，27(4)：398-401
[130] Kapuscinski J., SKOCZYLAS B., Simple and Rapid Fluorimetric Method for DNA Microassay[J]. Anal Biochem[J] , 1977,83(1) :252.
[131] Banville D.L., MarzilliL.G.,Wilson W.D., P-31 Nmr and Viscometric Studies Of the Interaction of Meso-Tetra(4-N-Methylpyridyl)Porphine and Its Ni(Ii) and Zn(Ii) Derivatives With DNA[J]. Biochem.Biophys. Res. Commun., 1983,113(1):148-162.
[132] Nakamura J., Igarashi S., Highly sensitive spectro- fluorometric determination of nucleic acid based on the aggregation of two oppositely charged kinds of water-soluble porphyrins[J]. Anal.Lett., 1996, 29(14): 2453-2461.
[133] Li, Wen-You, Xu, Jin-Gou, He, Xi-Wen. Determination of nucleic acids using rivanol as the fluorescent probe in the presence of SDS[J]. Analytical Letters, 2000, 33 (15): 3183-3194
[134] 贺吉香，江崇球，高明霞．盐酸小檗碱与脱氧核糖核酸相互作用的研究[J]．光谱学与光谱分析，2003，(4)：132-135
[135] 胡敏，杨欣，晋卫军，曹祉祥，沈国励，俞汝勤．荧光光谱法研究抗癌新药吡柔比星与DNA的相互作用[J]．药学学报，1999，34(8)：608-612
[136] Wen-You Li, Jin-Gou Xu, Xiang-Qun Guo, Qing-zhi Zhu, Yi-Bing Zhao. Application of vitamin kappa-3 as a photochemical fluorescence probe in the determination of nucleic acids[J]. Anal. Lett. 1997, 30(2), 245-257
[137] Wenyou Li,Xiangqun Guo, Jingou Xu, Qingzhi Zhu, Yibing Zhao. Determination of DNA using sodium 9,10-anthraquinone-2-sulfonate as an in situ photochemical fluorescence probe[J]. Anal. Chim. Acta, 1997, 340 (1-3) :291-296
[138] Yun-Xiang Ci, Yuan-Zong Li, and Xiao-Jing Liu, Selective Determination of DNA by its Enhancement Effect on the Fluorescence of the Eu-Tetracycline Complex[J]. Anal. Chem. 1995, 67(11): 1785-1788
[139] Yi-Bing Zhao, Dong-Yun Wang, Xiang Qun Guo, Jin Guo Xu. Determination of DNA and RNA by their quenching effect on the fluorescence of the Tb3+-Tiron complex[J]. Anal. Chim. Acta, 1997, 53(2-3): 329-335
[140] Jing Zhou, Guo-quan Gong, Ying-Nian Zhang, Jian-Qiang Qu, Liu-Fang Wang,Ji-Wu Xu, Quercetin-La(Ⅲ) complex for the fluorimetric determination of nucleic acids[J]. Anal. Chim. Acta,1999,38 I(1):17-22
[141] C. Z. Huang, K. A. Li, S. Y. Tong, Fluorescent Complexes of Nucleic Acids-8-Hydroxyquinoline-La(Ⅲ) and The Spectrofluorimetry of Nucleic Acids[J]. Anal. Lett., 1996, 29(10): 1705-1717
[142] Xia Wu, Jinghe Yang, Fang Huang, Min Wang, Limei Sun, Guiying Xu, Study of the reaction between nucleic acid and Tb-BPMPHD-CTMAB complex and its analytical application[J]. Anal. Lett., 1999,32(12): 2417-2425
[143] G. L. Wang, J. L. Yuan, K. Matsumoto, Z. Hu, Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA- Eu(III)-beta-diketonate ternary complex[J]. Anal. Biochem., 2001, 299(2): 169-172
[144] C. G. Lin, G. L. Zhang, J. H. Yang, Fluorimetric determination of nucleic acids with terbium and lutetium[J]. Microchem. J., 2002, 71(1): 9-14
[145] Bernard Juskowiak,Ilona Grzybowska, Elzbieta Galezowska,Shigeori Takenaka. Enhanced fluorescence of the Eu3+-naphthalenediimide derivative-phenanthroline ternary complex and the determination of DNA[J]. Anal. Chim. Acta,2004,512(1):133-139
[146] Wu X, Guo C, Yang J, Wang M, Chen Y, Liu J. The sensitive determination of nucleic acids using fluorescence enhancement of Eu3+-benzoyl acetonecetyltrimethylammonium bromide- nucleic acid system[J]. J Fluoresc. 2005,15(5):655-660.
[147] Yegorova A, Karasyov A, Duerkop A, Ukrainets I, Antonovich V. New luminescent terbium complex for the determination of DNA[J]. Spectrochim Acta A. 2005, 61(1-2):109-116.
[148] Zhu CQ, Zhuo SJ, Chen JL, Wu YQ, Li YX, Li DH, Zheng H, Xu JG A kinetic fluorometric method for the determination of nucleic acids using a ternary equilibrium system of nucleic acids - iron(III) tetracarboxy phthalocyanine - poly-lysine coupled with the oxidation reaction between hydrogen peroxide and DL-tyrosine[J]. Analytica Chimica Acta, 2004, 514 (2): 247-252
[149] Qiu-E Cao, Yunkun Zhao,Yunyuan Xu, Changzheng Li, Zhide Hu, and Qiheng Xu. Studies on the Fluorescence Reaction between Nucleic Acid and the Complex of Cobalt(II) with 5-(3-Fluoro-4-chlorophenylazo)-8-sulfonamidoquinoline and its Applications[J]. Analytical Biochemistry, 2000,277(2):214-220.
[150] Ling LS, He ZK, Song GW, Zeng YE, Wang C, Bai CL, Chen XD, Shen P. High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)2(dppx)2+ [J]. Analytica Chimica Acta, 2001,436 (2): 207-214.
[151] Lian-Sheng Ling, Zhi-Ke He, Gong-Wu Song, Din Yuan, Yun-e Zeng. A novel method for determination of DNA by use of molecular "Light Switch" complex of Ru(bipy)2(dppx)2+ [J]. Analytica Chimica Acta, 2000,403 (1-2): 209-217
[152] 朱展才，汪静，朱红斌．铌．桑色素荧光探针猝灭法测定核酸[J]．分析化学，2002，30(11)：1319-1321
[153] 龙朝阳，俞英，吴建中，孙姒巧．铜(Ⅱ)．邻菲咯啉-(邻菲咯啉-5，6-二酮)络合物的合成及其同脱氧核糖核酸的作用[J]．分析化学，2002，30(4)：392-394
[154] 王岩玲，杨周生．联吡啶一铜(Ⅱ)络合物荧光增强法测定核酸[J]．分析化学，2005，33(1)，57-59
[155] Yun-Xiang Ci,Yong Chen,Yuan-Zong Li,Wen-Bao Chang. The Use of Mn-TPPS4 Mimetic Peroxidase in a DNA Hybridization Assay[J]. Microchemical Journal, 1995,52(3): 257-262.
[156] 张延荣，宗志新，龚国权，孙彬，段海峰，穆德琪，徐喜莲．核酸-槲皮素-锌(Ⅱ)三元配合物荧光法测定核酸[J]．兰州大学学报(自然科学版)，2000，36(3)：100-104
[157] J. H. Yang, C. G. Lin, G. L. Zhang, Z.Q.Gao, X.Wu, X.R. Huang,Study on a fluorescence enhanced system of 1, 2, 4-trihydroxyanthro quinone-Be2+-DNA[J]. Spectrochim. Acta.A, 1998, 54(12): 2019-2024
[158] 林旭聪，谢增鸿，郭良洽，陈国南，核酸-桑色素·铝(Ⅲ)三元荧光体系的研究[J]．光谱学与光谱分析，2004，24(10)，1230-1234
[159] D. H. Li, X. L. Chen, Y. Fang, J. G. Xu, Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and acridine orange[J]. Analyst, 2001, 126(4): 518-522
[160] C. Z. Huang, Y. F. Li, S. Y. Tong, Spectrofluorimetry of Nucleic Acids on the Their Ternary Complexes of Aluminum(Ⅲ)/8-Hydroxyquinoline[J]. Anal. Lett., 1997, 30(7): 1305-1309
[161] 林旭聪，谢增鸿，郭良洽，林坚，陈国南．铟(Ⅲ)一8一羟基喹啉一核酸三元荧光体系的研究与应用[J]．光谱实验室，2003，20(6)810-814
[162] J. Lagona, P. Mukhopadhyay, S. Chakrabarti, L. Isaacs. The cucurbit[n]uril family[J]. Angew. Chem. Int. Ed. 2005,44(31):4844-4670
[163] R. Hoffmann, W. Knoche, C. Fenn, H.-J. Buschmann. Host-guest Complexes of Cucurbituril with the 4-Methylbenzylammonium Ion, Alkali-metal Cations and NH4+[J]. J. Chem. Soc. Faraday Trans. 1994,90 (11) 1507-1511.
[164] 李刚，冯亚青．葫芦脲[J]．化学通报，2005，68，w054(http://www.hxtb.org)
[165] W. A. Freeman, W. L. Mock, N. Y. Shih. Cucurbituril [J]. J.Am.Chem. Soc. 1981, 103(24): 7367-7368
[166] H.J. Buschmann, E. Cleve and E. Schollmeyer.Cucurbituril as a ligand for the complexation of cations in aqueous solutions[J]. Inorg. Chim. Acta. 1992,193(1):93-97
[167] H.-J. Buschrnann, E. Cleve, K. Jansen, A. Wego, E. Schollmeyer. Complex Formation between Cucurbit[n]urils and Alkali, Alkaline Earth and Ammonium Ions in Aqueous Solution[J]. J. Incusion. Phenom. Mol. Recognit. Chem. 2001,40(1-2), 117-120
[168] H. -J. Buschmann, E. Cleve, K. Jansen and E. Schollmeyer. Determination of complex stabilities with nearly insoluble host molecules: cucurbit[5]uril, decamethylcucurbit[5]uril and cucurbit[6]uril as ligands for the complexation of some multicharged cations in aqueous solution [J]. Anal.Chim.Acta 2001,437(1): 157-163
[169] W. L. Mock and N. Y. Shih. Host-guest binding capacity of eucurbituril[J]. J. Org. Chem., 1983,48(20):3618-3619
[170] William L. Mock and Neng Yang Shih. Structure and selectivity in host-guest complexes of cucurbituril[J]. J. Org. Chem.,1986,51 (20): 4440-4446.
[171] William L. Mock and Neng-Yang Shih. Organic Ligand-Receptor Interactions between Cucurbituril and Alkylammonium Ionstyl[J]. J. Am. Chem. Soc., 1988, 110(14), 4706-4710
[172] H.J. Buschmann, K. Jansen, E. Schollmeyer The formation of cucurbit[6]uril complexes with amino acids and amino alcohols in aqueous formic acid studied by calorimetric titrations[J]. Thermochimica Acta, 1998,317(1):95-98
[173] Buschmann H J, Schollmeyer E. Cucurbituril and β-cyclodextrin as hosts for the complexation of organic dyes[J]. J. Incl. Phenom. Mol. Reconit. Chem. 1997, 29:167-174
[174] Roland Neugebauer and Wilhelm Knoche. Host-guest complexes of cucurbituril with 4-amino-4'-nitroazobenzene and 4'-diaminoazo benzene in acidic aqueous solutions [J]. J. Chem. Soc., Perkin Trans.2 1998,3:529-534
[175] 杨守林，薛赛凤，马培华，祝黔江，陶朱，张建新．瓜环与4，4’-二氨基二苯甲烷衍生物的相互作用[J]．2005，25(1 0)：1215～1220
[176] 杜莹，薛赛凤，祝黔江，陶朱．紫外吸收光谱法测定瓜环与喹啉衍生物包结物包结比[J]．贵州大学学报(自然科学版)，2005，22(2)：165-168
[177] H-J Buschmann, T. Wolff, Fluorescence of 1-anilino naphthalene-8-sulfonate in solid macrocyclic environments[J]. Journal of Photochemistry and Photobiology A, 1999,121 (2): 99-103
[178] Brian D. Wagner and Andrew I. MacRae. The Lattice Inclusion Compound of 1,8-ANS and Cucurbituril: A Unique Fluorescent Solid[J]. J. Phys. Chem. B 1999, 103(46): 10114-10119
[179] Brian D. Wagner, Shannon J. Fitzpatrick, Monica A. Gill, Andrew I. MacRae, and Natasa Stojanovic. A fluorescent host-guest complex of cucurbituril in solution: a molecular Jack O'Lantern[J]. Can. J. Chem./Rev. Can. Chim. 2001,79(7): 1101-1104
[180] Brian D. Wagner,Natasa Stojanovic, Anthony I. Day, Rodney J. Blanch. Host Properties of Cucurbit[7]uril: Fluorescence Enhancement of Anilinonaphthalene Sulfonates[J]. J. Phys. Chem. B 2003, 107(39): 10741-10746
[181] Rankin, MA, Wagner, BD. Fluorescence enhancement of curcumin upon inclusion into cucurbituril[J]. Supramol Chem. 2004,16 (7): 513-519
[182] Sung Im Jun, Jae Wook Lee, Shigeru Sakamoto, Kentaro Yamaguchi and Kimoon Kim. Rotaxane-based molecular switch with fluorescence signaling[J]. Tetrahedron Letters, 2000, 41 (4): 471-475
[183] Mohanty, J, Nau, WM. Ultrastable rhodamine with cucurbituril[J]. Angew. Chem. Int. Edit. 2005, 44 (24): 3750-3754
[184] Nau, WM, Mohanty, J. Taming fluorescent dyes with cucurbituril[J]. Int J Photoenergy, 2005,7 (3): 133-141
[185] Jyotirmayee Mohanty and Werner M. Nau. Refractive index effects on the oscillator strength and radiative decay rate of 2,3-diazabicyclo[2.2.2] oct-2-ene[J]. Photochemical & Photobiological Sciences, 2004, 3(11-12): 1026-1031
[186] C. Marquez, F. Huang, W. M. Nau. Cucurbiturils: molecular nanocapsules for time-resolved fluorescence-based assays [J]. IEEE Trans. Nanobiosci. 2004,3 (1): 39-45
[187] C. Marquez, W. M. Nau. Polarizabilities Inside Molecular Containers [J]. Angew. Chem. Int. Ed. 2001, 40(23): 4387-4390.
[188] Lim YB, Kim T, Lee JW, Kim SM, Kim HJ, Kim K, Park JS. Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: noncovalent strategy in developing a gene delivery carrier[J]. Bioconjug Chem. 2002, 13(6):1181-1185.
[189] Rosen C G, Weber G. Dimer formation from 1-anlino-8-naphthlaene-sulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with exceptional binding properties[J]. Biochemistry,1969, 8(10):3915-3920
[190] 刘思敏 严砚 吴成泰 葫[n]环联研究进展．大环化学和超分子化学研究进2002．9(西安)
[191] H.J. Buschmann, E. Schollmeyer, L. Mutih. The formation of amino acid and dipeptide complexes with a-cyclodextrin and cucurbit[6]uril in aqueous solutions studied by titration calorimetry [J]. Thermochimica Acta 2003,399(l-2):203-208
[192] Brian D. Wagner and Shannon J. Fitzpatrick. A Comparison of the Host-Guest Inclusion Complexes of 1,8-ANS and 2,6-ANS in Parent and Modified Cyclodextrins [J]. J. Incl. Phenom. Macro. Chem. 2000, 38(1-4):467-478.
[193]X.L.Xu. Experiment of biochemistry [M].Beijing:Medical Technology of China Press, 1994,p31
[194] E. Gaggelli, F. Berti, N. D'Amelio, N. Gaggelli, G. Valensin, L. Bovalini, A. Paffetti, L. Trabalzini. Metabolic pathways of carcinogenic chromium, environmental health perspectives [J]. 2002, 110: 733-738.
[195] S. Caspi, E. Ben-Jacob. Conformation changes and folding of proteins mediated by Davydov's soliton [J]. Physics Letters A, 2000, 272: 124-129.
[196] W.S. Tao. Protein Macular Basic [M]. Beijing: The People's Education Press, 1981, p256.
[197] Patra, Digambara. Mishra.A.K.pp. Recent developments in multi-component synchronous fluorescence scan analysis[J]. TrAC Trends in Analytical chemistry.2002, 21(12):787-798
[198]Xiujuan Yang, Ju Chou, Gongquan Sun, Hui Yang and Tianhong Lu Synchronous Fluorescence Spectra of Hemoglobin: A Study of Aggregation States in Aqueous Solutions[J]. Microchemical J. 1998,60(3):210-216
[199] 孙长侠 博士学位论文 2005, p98
[200] T.D. Brown, T.M. Shepherd. Efficiencies of fluorescent Tb(III) chelates in the solid state [J]. J. Chem. Soc: Dalton Trans., 1973, 3: 336-341.
[201] Wu P.G, Brand L. Resonance Energy Transfer: Methods and Applications [J]. Anal.Biochem.,1994, 218(1):1-13
[202] R.M.Clegg. Fluorescence resonance energy transfer[J].Curr.Opin.In. Biotech, 1995,6(1):103-110
[203] G.A.Kumar,N.V. Unnikrishnan. Energy transfer and optical gain studies of FDS: RhB dye mixture investigated under cw laser excitation [J]. J. Photochem. Photobiol. A, 2001,144(2-3): 107-117
[204] 马春琪 李克安 赵凤林 童沈阳．牛血清白蛋白与铬天青S作用机理的研究[J]．化学学报1999．57(4)：389-395
[205] Denis G. Samsonenko, Janusz Lipkowski, Olga A. Gerasko, Alexander V. Virovets, Maxim N. Sokolov, Vladimir P. Fedin, Javier G. Platas, Rita Hernandez-Molina,and Alfredo Mederos. Cucurbituril as a New Macrocyclic Ligand for Complexation of .Lanthanide Cations in Aqueous Solutions [J]. Eur. J. Inorg. Chem. 2002, 2002(9):2380-2388
[206] 罗绪强，张桂玲，薛赛凤，陶朱，祝黔江．酸度及碱金属离子在瓜环合成分离中的应用研究[J]．贵州大学学报(自然科学版)2003，20(3)，303-305
[207] 沈星灿 何锡文 梁宏 纳米粒子特性与生物分析[J]．分析化学2003，31(7)，880-885
[208] 沈星灿，袁琦，梁宏，闫海刚，何锡文．血清白蛋白与银纳米粒子相互作用的滞后效应[J]．中国科学B辑，2002，33(2)，177-184
[209] 郭德华，王敏，李芳．高效液相色谱法测定水产品中的噁喹酸[J]．化学分析计量 2003，12(1)：24-25
[210] A. E. Tyrpenou, , G. Rigos Determination of Oxolinic Acid Residues in Gilthead Seabream (Sparus aurata) Muscle Tissue and Plasma by High-Performance Liquid Chromatography[J]. Chromatographia 2004,60 (11/12):657-661
[211] 陈国珍等 荧光分析法(第二版) 科学出版社 1990，p115
[212] Fierens, C.; Hillaert, S.; and Bossche W. V. The qualitative and quantitative determination of quinolones of first and second generation by capillary electrophoresis[J]. Journal of Pharmaceutical and Biomedical Analysis, 2000, 22(5), 763-772
[213] Cohen, E.; Maxwell, R. J.; and Donoghue, D. J. Automated multi-residue isolation of fluoroquinolone antimicrobials from fortified and incurred chicken liver using on-line microdialysis and high-performance liquid chromatography with programmable fluorescence detection[J]. Journal of Chromatography B, 1999, 724(1):137-145
[214] Yorke, J.C. and Froc, P. Quantitation of nine quinolones in chicken tissues by highperformance liquid chromatography with fluorescence detection[J]. J. Chromatogr. A, 2000, 882 (1-2): 63-77.
[215] Pouliquen, H. and Armand, F. Determination of oxolinic acid in faeces and urine of turbot (Scophthalmus maximus) by high-performance liquid chromatography using fluorescence detection[J]. Journal of Chromatography B, 2000, 749(1): 127-133
[216] Tyrpenou, A. E.; Rigos, G. Determination of Oxolinic Acid Residues in Gilthead Seabream (Sparus aurata) Muscle Tissue and Plasma by High-Performance Liquid Chromatography [J]. Chromatographia, 2004, 60(11-12): 657-661
[217] Loussouarn, S.; Pouliquen, H. and F. Armand. High-performance liquid chromatographic determination of oxolinic acid in the plasma of seabass (Dicentrarchus labrax) anaesthetized with 2-phenoxyethano[J]. J. Chromatogr. B 1997, 698(1-2): 251-259.
[218] Touraki, M.; Ladoukakis, M.; Prokopiou, C. High-performance liquid chromatographic determination of oxolinic acid and flumequine in the live fish feed Artemia [J]. Journal of Chromatography B, 2001,751 (2), 247-256
[219] Dorota, K. and Hanna, H. Application of derivative UV spectrophotometry for the determination of cinoxacin and oxolinic acid in pure and dosage form [J]. Chem.Anal. (Warsaw), 2003, 48 (1): 97-106.
[220] Liu, J.Q., Wang, X., and Zhao, H.C. Europium sensitized fluorescence assay of rufloxacin in human serum and urine [J]. Chinese Journal of Analysis Laboratory, 2001, 20 (4): 79-82.
[221] Tong, C.L., Xiang, G.H., Huang, D.J., and Liu, W.P. Determination of ofloxacin by the terbium(III) ion fluorescence probe sensitized by the surfactant[J]. Chinese Journal of Analytical Chemistry, 2004, 32 (5): 619-621.
[222] Wu, S.Q., Cao, Q.E., Zhao, Y.K., Zhang, W.J., Chen, X.G., and Hu, Z.D. The fluorescence characteristic of Tb-ciprofloxacin and the determination of ciprofloxacin[J]. Chinese Journal of Analytical Chemistry, 2000,28 (12): 1462-1466.
[223] 王静萍，杜黎明，段亚丽．铽．依诺沙星荧光特性研究及应用[J]．分析化学，2004，32(1)：50-52
[224] 潘建章，李树伟，何艺，苏小东，王小红．铽．司帕沙星荧光体系的研究及应用[J]．四川师范大学学报(自然科学版)，2005，28(1)：101-103
[225] 戚雪勇，何华，顾飞，余江河．铽离子增敏环丙沙星研究及其应用[J]．中国药学杂志，2005，40(19)：64-67
[226] 周静，杜黎明，陈彩萍．铽-培氟沙星的荧光特性研究及培氟沙星的测定[J]．光谱学与光谱分析．2004，24(6)：78-80
[227] Beltyukova S.V.; Egorova A.V. Terbium chelates for fluorescence immunoassays[J]. J. Pharm. Biomed. Anal., 1998,18 (1-2): 267-270.
[228] 李元宗，常文保．生化分析[M]．高等教育出版社，2003，pp146
[229] The Public Health Department of People's Republic of China. Pharmacopoeia of the People's Republic of China[M]. Beijing: Chemical Industry Press, 1995(1): 311-312.
[230] W. Q. Sun, J. F. Sheng. Handbook of Natural Active Constituents[M]. Beijing: Chinese Science and Technology Press, 1998, pp2240-2316.
[231] Y.X. Peng, W.H. Zhu, J.H. Chen, Experimental Biochemistry[M]. The People's Education Press, 1989, pp. 79-80.
[232] S.J. Qin, J. S. Shen,Y. Y. Li, Y. J. Wei. Study on combination of the rare-earth metal ions and bovine serum albumin[J]. Journal of Hebei normal university(Natural Science Edition), 2003, 27(3): 274-277.
[233] Xiao-jing Liu, Yuan-zong Li, Yun-xiang Ci. Time-resolved fluorescence studies of the interaction of the Eu3+ complexes of tetracycline analogues with DNA[J]. Anal. Chim. Acta, 1997, 345(1-3): 213-217.
[234] 朱庆枝，李芳，郭详群，许金钩，李文友．竹红菌素A与DNA作用的研究[M]．分析化学新进展(山西科技出版社)，1997，pp244-245
[235] A. M. Pyle, J. P. Rehmann, R. Meshoyrer, C. V. Kumar, N. J. Turro, Jacqueline K. Barton. Mixed-ligand complexes of ruthenium(II): factors governing binding to DNA [J]. J. Am. Chem. Soc, 1989, 111(8): 3051-3058.
[236] A. Nakajima. A study on the system of pyrene and β-cyclodextrin in aqueous solution utilizing the intensity enhancement phenomenon[J]. Spectrochim. Acta. A, 1983, 39(10): 913-915.
[237] Y. C. Jiang, S. K. Wu. A study on the micellar viscosity in surfactant solution by fluorescence polarization[J]. Photographic science and photochemistry, 1995, 13(2): 180-185.
[2] Ohning GV, Neet KE. 6-(p-toluidinyl)naphthalene-2-sulfonic acid as a fluorescent probe of yeast hexokinase: conformational states induced by sugar and nucleotide ligands[J]. Biochemistry. 1983,22(12):2986-2995.
[3] R. F. Steiner, H. Edelhoch, The Properties of Thyroglobulin. Ⅵ. The Internal Rigidity of Native and Denatured Thyroglobulin [J]. J. Am. Chem. Soc., 1961, 83(6): 1435-1444.
[4] G. R. Shephenol, B. J. Noland. A fluorescence assay suitable for histone solutions [J]. Anal. Biochem., 1965, 11(3): 443-448.
[5] T.H. Steinberg, R.P. Haugland, V. Singer. Applications of sypro orange and sypro red protein gel stains [J]. Anal. Biochem., 1996, 239(2): 238-245.
[6] 刘保生，高静，杨更亮．吖啶橙罗丹明6G荧光共振能量转移及其罗丹明6G荧光猝灭法测定蛋白质[J]．分析化学，2005，33(4)：546-548
[7] 朱英贵，高峰，章丽，谢筱娟，王伦．新荧光试剂1-甲酰基苯并三唑缩邻氨基苯酚的合成及其在蛋白质测定中的应用[J]．分析化学，2005，33(5)：157
[8] Cui FL, Fan J, Li JP, Hu ZD Interactions between 1-benzoyl-4-pchlorophenyl thiosemicarbazide and serum albumin: investigation by fluorescence spectroscopy [J]. Bioorganic & Medicinal Chemistry. 2004, 12 (1): 151-157
[9] Chen Y, Yao MN, Tang Y J, Li YQ. Determination of protein with TPPS by total internal reflection synchronous fluorescence spectroscopy[J]. Spectroscopy and Spectral Analysis 2005, 25 (12): 2048-2051
[10] A. Sukowska, J. Rownicka, B. Bojko and W. Sukowski. Interaction of anticancer drugs with human and bovine serum albumin[J]. Journal of Molecular Structure 2003, 651-653:133-140
[11] Anna Sukowska. Interaction of drugs with bovine and human serum albumin[J].Journal of Molecular Structure, 2002,614(1-3): 227-232
[12] Wen-wu Qina, Guo-quan Gonga and Yu-min Song. Determination of proteins by fluorescence quenching of Magdala Red[J]. Spectrochimica Aeta Part A: 2000,56(5): 1021-1025
[13] Claire Dufour, Olivier Dangles. Flavonoid-serum albumin complexation: determination of binding constants and binding sites by fluorescence spectroscopy[J]. Biochimica et Biophysica Acta, 2005, 1721(1-3): 164-173
[14] Y.J. Luo, H.X. Shen. Study on Acridine Orange dimer as a new fluorescent probe for the determination of protein [J]. Anal. Commun., 1999, 36(4): 135-138.
[15] 陈鸿琪，夏闽．荧光染料吖啶红作测定蛋白质的生物探针[J]．理化检验一化学分册，2001，37(2)：53-55
[16] 高峰，朱昌青，汪乐余，王伦．灿烂甲酚蓝与表面活性剂的作用及其在蛋白质测定中的应用[J]．分析化学 2002，30(3)：324-326
[17] J. C. Garcia-Borron, J. Escribano, M. Jimenez and J. L. Iborra. Quantitative determination of tryptophanyl and tyrosyl residues of proteins by second-derivative fluorescence spectroscopy[J]. Analytical Biochemistry, 1982, 125 (2):277-285
[18] 高峰，朱昌青，王伦．耐尔蓝-十二烷基苯磺酸钠-蛋白质体系的荧光反应及其分析应用[J]．分析化学 2003，3 1(9)：1085-1088
[19] J. B. Joseph. Fluorometric microdetermination of human serum albumin [J]. Anal. Chem., 1960, 32(4): 560-563.
[20] Y.X. Ci, L. Chen. Fluorimetric determination of human serumal bumin with eriochromecyanine [J]. Analyst, 1988, 113 (4): 679-681.
[21] S. Takaji, O. Tatsuya, I. Kayo, K. Kazumi, M. Naomi, C. Massahiko, S. Yutaka, K. Massao, N. Sonko, I. Takateru. Determination of trace amounts of albumin in human bronchoalveolar lavage fluids by fluorometry with chromazurol S [J]. Clin. Chem. Acta, 1994, 229(1-2): 37-47.
[22] 张明翠，汪乐余，李玲，陈红旗，张德兴，王伦．新荧光试剂5-(4-羧基苯偶氮)-8-水杨醛缩氨基喹啉荧光增敏法测定人血清蛋白质[J]．分析化学，2004，32(3)：74-76
[23] T. Kinoshita, J. Murayama, A. Tsuji. Fluorophotometric assay of pr eins using hypochlorite-thiamine reagent [J]. Chem. Pharm. Bull., 1976, 24(11): 2901-2902.
[24] T. Kinoshita, J. Murayama, K. Murayama, A. Tsuji. Microanalysis of proteins and peptides novel method for the fluorometric assay of proteins using hypochlorite-thiamine reagent [J]. Chem. Pharm. Bull., 1980, 28(2): 641-644.
[25] C.Q. Ma, K.A. Li, S.Y. Tong. Determination of proteins by fluorescence quenching of erythrosin B [J]. Anal. Chim. Acta, 1996, 333(1-2): 83-88.
[26] T.H. Steinberg, R.P. Haugland, V. Singer. Applications of sypro orange and sypro red protein gel stains [J]. Anal. Biochem., 1996, 239(2): 238-245.
[27] N. Li, K.A. Li, S.Y. Tong. Fluorometric determination for micro amounts of albumin and globulin fractions without separation by using α, β, γ, δ,-tetra(4'-carboxyphenyl) porphin [J]. Anal. Biochem., 1996, 233 (2): 151-155.
[28] H. Ichiba, H. Niwayama, T. Yajima. High-sensitive flow-injection analysis of proteins coupled with online alkaline-hydrolysis and the fluorescent derivatization method [J]. Chem. Pharm. Bull., 1989, 37(11): 3155-3157.
[29] L.B. Maria. Protein assay in the microgram range: Modifications of the hiraoka-glick method [J]. Anal. Biochem., 1973, 53(1): 12-20.
[30] 李东辉，杨黄浩，郑洪，陈宾，陈秋影，许金钩．用四磺基铝酞菁直接测定血清中自蛋白[J]．分析化学，1999，27(9)：1018-1021．
[31] D.H. Li, H.H. Yang, H. Zhen, Y. Fang, Q.Z. Zhu, J.G. Xu. Fluorimetric determination of albumin and globulin in human serum using tetra-substituted sulphonated aluminum phthalocyanine [J]. Anal. Chim. Acta, 1999, 401(1-2): 185-189.
[32] M. J. Baar, G. Patonay. Ultrasensitive detection of closely related angiotensin i peptides using capillary electrophoresis with near-infrared laser-induced fluorescence detection [J]. Anal. Chem., 1999, 71 (3): 667-671.
[33] B. L. Legender Jr., D.L. Moberg, D.C. Williams, S.A. Soper. Ultrasensitive near-infrared laser-induced fluorescence detection in capillary electrophoresis using a diode laser and avalanche photodiode [J]. J. Chromatogr. A, 1997, 779 (1-2): 185-194.
[34] A. Chopdar, A.M. Turk, D.W. Hill. Fluorescent infra-red angiography of the fundusoculi using indocyanine green dye [J]. Trans. Ophthal. Soc., 1978, 98:142-1146.
[35] D. A. Wilberforce, G. Patonay. Investigation of near-infrared laser dye albumin complexes [J]. Spectrochim. Acta Part A, 1990, 46(8): 1153-1162.
[36] 郑洪，李江辉．合成的近红外花菁染料测定痕量血清蛋白质[J]．高等学校化学学报，1999，20(4)：555-557．
[37] M.A. Kessler, O.S. Wolfbeis. Eur. Pat. Appl., 1991, 20: 678.
[38] E. Terpetsching, H. Szmacinski, J.R. Lakowicz. Synthesis, spectral properties and photostabilities of symmetrical and unsymmetrical squarines; a new class of flourophores with long-wavelength exiciation and emission [J]..Anal. Chim. Acta, 1995, 282(3): 633-641.
[39] E. Terpetschnig, H. Szmacinski, A. Ozinskas, J.R. Lakowicz. Synthesis of squaraine-n-hydroxysuccinimide esters and their biological application as long-wavelength fluorescent labels [J]. Anal. Biochem., 1994, 217(2): 197-204.
[40] T. Imasaka, T. Fuchigami, N. Ishibashi. Micellar Electrokinetic capillary chromatography of porphinato chelates as a spectrophotometric approach to sub-femtomole detection of metal chelates [J]. Anal. Sci., 1991, 7(3): 491-498.
[41] T. Higashijima, T. Fuchigami, T. Imasaka, N. Ishibashi. Determination of amino acids by capillary zone electrophoresis based on semiconductor laser fluorescence detection [J]. Anal. Chem., 1992, 64(7): 711-714.
[42] A. J. G. Mank, H. T. C Vander Laan, H. Lingeman, C. Gooijer, U.A.Th. Brinkman, N.H. Velthorst. Visible diode laser-induced fluorescence detection in liquid chromatography after precolumn derivatization of amines [J]. Anal. Chem., 1995, 67(10): 1742-1748.
[43] M. B. Brown, T. E. Edmonds, J.N. Miller, D.P. Riley, N.J. Scare. Novel instrumentation and biomedical applications of very near infrared fluorescence [J]. Analyst, 1993, 118(4): 407-410.
[44] Zhu CQ, Wu YQ, Zheng H, Chen JL, Li DH, Li SH, Xu JG.. Near-infrared fluorometric determination of protein by shifting the ion-association equilibrium between cationic heptamethylene cyanine and poly-glutamate[J]. Analytical Letters, 2004, 37 (6): 1115-1127
[45] Patonay G, Kim JS, Kodagahally R, Strekowski L. Spectroscopic study of a novel bis(heptamethine cyanine) dye and its interaction with human serum albumin[J]. Applied Spectroscopy, 2005, 59 (5): 682-690
[46] 王守业，余华明，张祖德，刘清亮．荧光探针在蛋白质研究中的应用[J]．大学化学，1998，13(3)：5-10
[47] Breen P J, Hild EK, Horrocks WD Jr. Spectroscopic studies of metal ion binding to a tryptophan-containing parvalbumin[J]. Biochemistry, Biochemistry. 1985, 24(19): 4991-4997.
[48] 刘清亮，徐晓龙，王淳，谢波平，余华明．稀土及过渡金属离子对蛇毒内抗凝血因子荧光光谱的影响[J]．中国稀土学报，1992，10(2)：106-109
[49] J. H. Yang, G.J. Zhou, G.L. Zhang, Z.K. Si, J.T. Hu. Determination of some cephalosporins in pharmaceutical formulations by a fluorescence quenching method [J]. Anal. Commun., 1996, 33(5): 167-170.
[50] Changxia Sun, Jinghe Yang, Xia Wu, The fluorescent enhancement effect in terbium-gadolinium-protein-cetylpyridine bromide system and its application for the determination of protein at nanogram level[J]. Biochimie, 2004, 86(8): 569-578
[51] Changxia Sun, Jinghe Yang, Xia Wu,Benyu Su,Shufang Liu ,The fluorescent enhancement effect in terbium-gadolinium-protein- cetylpyridine bromide system and its application for the determination of protein at nanogram level[J]. Chem. Phys. Lett;2004,398 (4-6):343-350
[52] 黄淑萍，陈亮，李玉红．比较与研究金属离子与三种蛋白的配位机理[J]．光谱学与光谱分析，1995，15(5)：85-89
[53] X. D. Yang, Y.X. Ci, W.B. Chang. Time-resolved fluorescence immunoassay with measurement of a europium chelate in solution: dissociation conditions and application for determination of cortisol [J]. Anal. Chem., 1994, 66 (15): 2590-2594.
[54] Y.X. Ci, W.B. Wang, X.D. Yang. Fluorescence labelling with europium chelate of β-diketones and application in time-resolved fluoroimmunoassays (TR-FIA) [J]. Journal of Immunological Methods, 1995, 179(2): 233-241
[55] X. D. Yang, Y.X. Ci, W.B. Chang. Time-resolved fluorescence immunoassay with measurement of a europium chelate in solution: dissociation conditions and application for determination of cortisol [J]. Anal. Chem., 1994, 66 (15): 2590-2594.
[56] Y.X. Ci, W.B. Wang, X.D. Yang. Fluorescence labelling with europium chelate of β-diketones and application in time-resolved fluoroimmunoassays (TR-FIA) [J]. Journal of Immunological Methods, 1995, 179(2): 233-241
[57] ChangyingGuo, Xia Wu, Jingheyang, Fei Wang, Zhen Jia, Dehuan Ran, Jinhua Zheng. Determination of proteins using fluorescence enhancement of Tb-benzoylacetone-sodium dodecyl benzene sulfonate- protein system[J]. J. photochem. Photobio. A 2005 Nov. 29 online
[58] 史华红，杨燕生，龚孟濂，陈泮森．铽标记人血清白蛋白的荧光光谱及应用，发光学报，1996，17(9)：240-244
[59] A. Canfi, M. P. Bailey, B. F. Rocks. Multiple labeling of immunoglobulin G, Albumin and testosterone with a fluorescent Terbium chelate for fluorenscence immunoassays [J]. Analyst, 1989, 114(11): 1407-1411.
[60] R.A. Evangelista, A. Pallak, B. Allore, E.F. Yernplaton, R.C. Motin, E.P. Diamandis. A new europium chelate for protein labelling and time-resolved fluorometric applications [J]. Clin. Biochem., 1988, 21(3): 173-178.
[61] E. Reichstein, Y. Shami, M. Ramjeesingh, E. P. Diamandis. Laser-excited time-resolved solid-phase fluoroimmunoassays with the new europium chelate 7-bis(chlorosulfophenyl)- 1,10-phenanthroline -2,9-dicarboxylic acid as label[J]. Anal. Chem., 1988,60(10): 1069-1074.
[62] Shufang Liu, Jinghe Yang, Xia Wu, Fluorometric determination of proteins using the yttrium(Ⅲ)-Sodium Lauryl Sulfate-Rutin-protein system[J]. J.Luminescence, 2006, 117(2):217-224.
[63] Jia Z, Yang JH, Wu X, et al. Study on a fluorometric method for the determination of protein in serum using quercetin-lanthanum (Ⅲ)-sodium dodecyl benzene sulfonate-protein system[J]. Anal. Lett. 39 (1): 67-81 2006
[64] 高建华，翟海云，陈彬．杯[8]芳烃与铈D形成的镧系超分子作荧光探针测定蛋白质[J]．分析化学，2002，30(3)：295-297
[65] Fei Wang, Jinghe Yang,Xia Wu, Fluorescence Enhancement effect for the determination of proteins with Morin-Al3+- Cetyltrimethylammonium Bromide[J], Talanta,2005, 67(4):836-842
[66] 杨美玲，杨培菊，宋玉民．芦丁金属配合物的合成、表征及与血清白蛋白的相互作用[J]．无机化学学报，2005，21(4)483-489
[67] M. B. Brown Jr., M. Moronne, P. Gin, S. Weiss, A.P. Alivisatos. Semiconductor nanocrystals as fluorescent biological labels [J]. Science, 1998, 281: 2013-2016.
[68] Marcel Bruchez Jr., Mario Moronne, Peter Gin, Shimon Weiss, A. Paul Alivisatos. Semiconductor Nanocrystals as Fluorescent Biological Labels[J]. Science,1998, 281 (5385) : 2013-2016.
[69] 徐力，郭轶，解仁国，庄家骐，王连英，杨文胜，李铁津·水相合成CdS纳米晶标记牛血清白蛋白[J]．功能材料与器件学报2003，9(2)：201-204
[70] Zhu CQ, Zhao DH, Chen JL, Li YX, Wang LY, Wang L, Zhou YY,Zhuo S J, Wu YQ. Application of L-cysteine-capped nano-ZnS as a fluorescence probe for the determination of proteins[J]. Analytical and Bioanalytical Chemistry, 2004, 378 (3): 811-815
[71] Wang L, Chen HQ, Wang LY, Li L, Xu FG, Liu JS, Zhu CQ. Preparation and application of a novel composite nanoparticle as a protein fluorescence probe[J]. Analytical Letters, 2004, 37 (2): 213-223
[72] Wang LY, Kan XW, Zhang MC, Zhu CQ, Wang L. Fluorescence for the determination of protein with functionalized nano-ZnS[J]. Analyst, 2002, 127(11): 1531-1534
[73] E.A. Medcalf, D.J. Newman, E.G. Gorman, C.P. Proce. Rapid, robust method for measuring low concentrations of albumin in urine [J]. Clin. Chem., 1990, 36(3): 446-449.
[74] J.R. Taylor, M.M. Fang, S. Nie. Probing specific sequences on single DNA molecules with bioconjugated fluorescent nanoparticles [J]. Anal. Chem., 2000, 72(9): 1979-1986.
[75] Wang LY, Wang L, Dong L, Bian GR, Xia TT, Chen HQ. Direct fluorimetric determination of gamma-globulin in human serum with organic nano- particle biosensor [J]. Spectrochimica acta Part A, 2005, 61 (1-2): 129-133
[76] 敖丽梅，高峰，潘碧峰，崔大祥，古宏晨．研究金纳米粒子和牛血清白蛋白以及羊抗兔免疫球蛋白G的相互作用[J]．Chinese Journal of Chemistry，2006，(2)：253-256
[77] Evgenia Matveeva, Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski and Joseph R. Lakowicz. Metal-enhanced fluorescence immunoassays using total internal reflection and silver island-coated surfaces [J]. Analytical Biochemistry, 2004, 334(2): 303-311.
[78] Beverloo HB, van Schadewijk A, Bonnet J, van der Geest R, Runia R, Verwoerd NP, Vrolijk J, Ploem JS, Tanke HJ. Preparation and microscopic visualization of multicolor luminescent immunophosphors. Cytometry[J]. 1992, 13(6): 561-70.
[79] T. Toyooka, M. Ishibashi, T. Terao. Sensitive determination of n-terminal prolyl peptides by high-performance liquid-chromatography with laser-induced fluorescence detection [J]. J. Chromatogr. A, 1994, 661(1-2): 105-112.
[80] D.S. Stegehuis, U.R. Tjaden, C.M.B. Van den Beld, J. Van der Greef. Bioanalysis of the neuropeptide des-enkephalin-γ-endorphin by high-performance liquid chromatography with on-line sample pretreatment using gel permeation and solid-phase isolation [J]. J. Chromatogr. A, 1991, 549:185-193.
[81] V.H. Cohn, J.Lyle, A fluorometric assay for glutathione [J]. Anal. biochem., 1966, 14(3): 434-440.
[82] R. L. White, A. C. DeMarco, S. Shapiro, L. C. Vining, S. Wolfe. Measurement of 8-(L-a-aminoadipyl)-L -cysteinyl-D-valine synthetase activity in Streptomyces clavuligerus by high-performance liquid chromatography after precolumn derivatization with o-phthaldialdehyde [J]. Anal. Biochem., 1989, 178(2): 399-403.
[83] G. Szokan. Precolumn derivatization in hplc of amino-acids and peptides [J]. J. Liq. Chromatogr., 1982, 5(8): 1493-1498.
[84] H. Koning, H. Wolf, K. Venema, J. Korf. Automated procolumn derivatization of amino acids, small peptides, brain amines and drugs with primary amino groups for reversed-phased high-performance liquid chromatography using naphthalenedialdehyde as the fluorogenic label [J]. J. Chromatogr., 1990, 533: 171-178.
[85] D.M. Pinto, E.A. Arriaga, D. Craig, J. Angelova, N. Sharma, H. Ahmadzadeh, N.J. Dovichi, C.A. Boulet. Picomolar assay of native proteins by capillary electrophoresis precolumn labeling, submicellar separation, and laser-induced fluorescence detection [J]. Anal. Chem., 1997, 69(15): 3015-3021.
[86] J.P. Liu, Y.Z. Hsieh, D. Wiesler, M. Novotny. Design of 3-(4-carboxybenzoyl) -2- quinolinecarboxaldehyde as a reagent for ultrasensitive determination of primary amines by capillary electrophoresis using laser fluorescence detection [J]. Anal. Chem., 1991, 63(5): 408-412.
[87] A.F. Lehninger. Biochemie 2 [M].Auflage: Verlag Chem Weinheim, 1979.
[88] A.M. Felix, M.H. Jimenez. Rapid fluorometric detection for completeness in solid phase coupling reactions [J]. Anal. Biochem., 1973, 52(2): 377-381.
[89] Y. Hiraga, T. Kinoshita. Post-column derivatization of guanidino compounds in high-performance liquid-chromatography using ninhydrin [J]. J. Chromatogr., 1981, 226(1): 43-51.
[90] G.R. Rhodes, V.K. Boppana. High-performance liquid- chromatographic analysis of arginine-containing peptides in biological-fluids by means of a selective post-column reaction with fluorescence detection [J]. J. Chromatogr., 1988,444: 123-131.
[91] C.M.B. van den Beld, U.R. Tjaden, N.J. Reinhoud, D.S. Stegehuis, J. van der Greef. Determination of some P-endorphin fragments in human plasma by high-performance liquid chromatography with laser induced fluorescence detection [J]. J. Control. Release, 1993, 13: 129-139.
[92] V. Fierabracci, P. Masiello, M Novelli. Application of amino-acid-analysis by high-performance liquid- chromatography with phenyl isothiocyanate derivatization to the rapid-determination of free amino-acids in biological samples [J]. J. Chromatogr., 1991, 570(2): 285-291.
[93] S.A. Cohen, D.P. Michaud. Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography [J]. Anal. Biochem., 1993, 211 (2): 279-287.
[94] M. Albin, R. Weinberger, E. Sapp, S. Moring. Fluorescence detection in capillary electrophoresis: evaluation of derivatizing reagents and techniques [J]. Anal. Chem., 1991, 63(5): 417-422.
[95] H. Bruckner, M. Lupke. Use of chromogenic and fluorescent oxycarbonyl chlorides as reagents for amino-acid-analysis by high-performance liquid-chromatography [J]. J. Chromatogr. A, 1995, 697(1-2): 295-307.
[96] V. Fierabracci, P. Masiello, M. Novelli, E. Bergamini. Application of amino acid analysis by high-performance liquid chromatography with phenyl isothiocyanate derivatization to the rapid determination of free amino acids in biological samples [J]. J. Chromatogr. B., 1991, 570 (2): 285-291.
[97] M.A. Cichy, D.L. Stegmeier, H. Veening, H.D. Becker. High-performance liquid chromatographic separation of biogenic polyamines using 2-(1-pyrenyl)ethyl chloroformate as a new fluorogenic derivatizing reagent [J]. J. Chromatogr. B. 1993, 613 (1): 15-21.
[98] A.J. Faulkner, H. Veening, H.D. Becker, 2-(9-Anthryl)ethyl chloroformate: a precolumn derivatizing reagent for polyamines determined by liquid chromatography and fluorescence detection [J]. Anal. Chem., 1991, 63(3): 292-296.
[99] Huang C.Z., Li K.A., Tong S.Y., Determination of Nanograms of Nucleic Acids by Their Enhancement Effect on the Resonance Light Scattering of the Cobalt(Ⅱ)/4-[(5-Chloro-2-pyridyl)azo]- 1,3-diaminobenzene Complex[J]. Anal. Chem.,1997,69(3): 514--520
[100] Huang C.Z., Zhu J.X., Li K.A., Tong S.Y., Determination of Albumin and Globulin at Nanogram Levels by a Resonance Light-Scattering Technique with α,β,γ,δ-Tetrakis(4-sulfophenyl)porphine[J]. Anal. Sci., 1997, 13(2): 263-268
[101] Anglister J, Steinberg I. Z. Depolarized Rayleigh light scattering in absorption bands measured in cycopene solution[J]. Chem. Phys. Lett., 1979,65(1):50-54
[102] 王亚婷，赵凤林，李克安，童沈阳 有机染料作为共振光散射探针在生物分析中的研究进展[J]．高等学校化学学报，2000，21(10)：1491-1497
[103] 王甫丽，郝英霞，张红蕾，赵勇，刘保生，张红医．苯酚磺酞类酸性染料与蛋白质包合物的共振散射光谱[J]．河北大学学报(自然科学版)：2002，22(1)：32-35
[104] C.Z. Huang, Y.F. Li, J.G. Mao, D.G. Tan. Determination of protein concentration by enhancement of the preresonance light-scattering of α,β,γ,δ-tetrakis (5-sulfothienyl) porphine[J]. Analyst, 1998, 123(6): 1401-1406.
[105] C. Q. Ma, K.A. Li, S.Y. Tong. Microdetermination of proteins by resonance light scattering spectroscopy with bromophenol blue [J]. Anal. Biochem., 1996, 239(1): 86-91.
[106] Z. G.Chen, T.Y. Zhang, F.L. Ren, W.F. Ding. Determination of Proteins at Nanogram Levels Based on Their Resonance Light Scattering Decrease Effect on the Dibromo-o-Nitrophenylfluorone-Sodium Lauroyl Glutamate Systema[J]. Microchim Acta, 2006,153 (1-2): 65-71
[107] 李树伟，李娜，赵凤林，李克安．用铀试剂Ⅰ共振光散射法测定蛋白质的研究[J]．光谱学与光谱分析，2002，22(4)：619-622
[108] 刘保生，张红医，张红蕾，赵勇．氯酚红-蛋白体系的共振光散射法定量测定蛋白质[J]．2003，23(2)：229-231．
[109] 杨传孝，李原芳，黄承志．，丽春红G用于人血清样品中总蛋白的共振光散射测定[J]．分析化学，2003，31(2)，148-152．
[110] Li yuanfang,Huang Chengzhi,Li Ming. A Resonance Light Scattering Determination of Proteins with Fast Green FCF[J]. Anal. Sci., 2002, 18, 177-181.
[111] Rutao Liu, Jinghe Yang, Xia Wu, Zengjun Lan, Resonance double light scattering method for the determination of proteins with morin/CTMAB [J]. Spectrochimica Acta Part A, 2002, 58(14):3077-3083
[112] Y.J. Chen, J.H. Yang, X. Wu, T. Wu, Y.X. Luan. Resonance light scattering technique for the determination of proteins with resorcinol yellow and OP [J]. Talanta, 2002, 58(5): 869-874.
[113] Xia Wu, Shuna Sun, Changying Guo, Jinghe Yang, Changxia Sun, Chengrong Zhou, Tao Wu. Resonance light scattering technique for the determination of proteins with Congo red and Triton X-100 [J]. Luminescence, 2006,21(1): 56-61
[114] Suling Feng, Zihong Pan and Jing Fan Determination of trace proteins with pyronine Y and SDS by resonance light scattering [J]. Analytical and Bioanalytical Chemistry, 2005,383(2):255-260
[115] Rutao Liu, Jinghe Yang, Changxia Sun, Xia Wu, Lei Li, Zhengmin Li, Resonance Light Scattering Method for the Determination of BSA and HSA with Sodium Docecyl Benzene Sulfonate or Sodium Laury Sulfate[J]. Anal, Bioanal. Chem., 2003,377 (2):375-379
[116] Lun Wang,Yongxin Li , Determination of proteins based on their resonance light scattering enhancement effect on manganese-tetra-sulfonatophthalocyanine[J]. Microchimica Acta ,2003, 143(4):275-279
[117] R.P. Jia, L.J. Dong, Q.F. Li, X.G. Chen, Z.D. Hu, Y. Nagaosa, Study of the interaction of protein and dibromomethyl-Arsenazo-Al(Ⅲ) by Rayleigh light-scattering techniqu[J]. Anal. Chim. Acta, 2001,442 (2): 249-256.
[118] L. J. Dong, Run Ping Jia, Qian Feng Li, X. G. Chen, Zhi De Hu, M. A. Hooper. Microdetermination of proteins with the arsenazo-DBN-Al(Ⅲ) complex by Rayleigh light-scattering technique and application of the method[J]. Fresenius' Journal of Analytical Chemistry 2001,370(8):1009- 1014
[119] Run-Ping Jia, Li-Jun Dong, Qian-Feng Li, Xing-Guo Chen and Zhi-De Hu. A highly sensitive assay for protein with dibromochloro -arsenazo-A13+ using resonance light scattering technique and its application[J]. Talanta, 2002,57(4): 693-700
[120] 许发功，王伦，汪乐余，陈红旗，张莉，陶海升．ZnS-Zn2+纳米溶胶作为共振光散射探针检测γ2G球蛋白[J]．分析科学学报，2005，21(3)：265-267
[121] 许发功，王伦，汪乐余，陈红旗，张莉，刘金水．利用CuS／Cu2+纳米溶胶作为共振光散射探针检测γ-球蛋白[J]．光谱学与光谱分析，2005，25(7)：1095-1098
[122] Le-Yu Wang, Lun Wang, Ling Dong, Yan-Li Hu, Ting-Ting Xia, Hong-Qi Chen, Ling Li and Chang-Qing Zhu. Determination of γ-globulin at nanogram levels by its enhancement effect on the resonance light scattering of functionalized HgS nanoparticles[J]. Talanta, 2004,62(2):237-240
[123] 张萍，卓淑娟，陈金龙，汪乐余，朱昌青．藉L-半胱氨酸包覆的ZnS纳米粒子的共振光散射定量分析蛋白质分析试验室[J]．2005，24(3)：86-90
[124] 梁爱惠，邹节明，蒋治良，王力生，覃爱苗．磷钼杂多酸与蛋白质相互作用的共振散射光谱研究[J]．应用化学，2002，19(8)：768-771
[125] 夏传俊，张莉，张锐，赵广超．纳米银胶与蛋白质的相互作用及应用[J]．安徽师范大学学报(自然科学版)，2003，26(2)：146-148
[126] Zheng Ping Li, Xin Rui Duan, Cheng Hui Liu and Bao An Du. Selective determination of cysteine by resonance light scattering technique based on self-assembly of gold nanoparticles[J]. Analytical Biochemistry, 2006, 351(1): 18-25
[127] Zhenxin Wang, Jason Lee, Andrew R. Cossins, and Mathias Brust. Microarray-Based Detection of Protein Binding and Functionality by Gold Nanoparticle Probes[J]. Anal. Chem., 2005,77(17):5770-5774
[128] M. J. Waring, Complex formation between ethidium bromide and nucleic acids[J]. J. Mol. Biol., 1965, 13(1): 269-282
[129] 何品刚，孙星炎，徐春，刘盛辉，陈奕利，方禹之．脱氧核糖核酸与双苯甲亚胺相互作用的荧光特性研究[J]．分析化学，1999，27(4)：398-401
[130] Kapuscinski J., SKOCZYLAS B., Simple and Rapid Fluorimetric Method for DNA Microassay[J]. Anal Biochem[J] , 1977,83(1) :252.
[131] Banville D.L., MarzilliL.G.,Wilson W.D., P-31 Nmr and Viscometric Studies Of the Interaction of Meso-Tetra(4-N-Methylpyridyl)Porphine and Its Ni(Ii) and Zn(Ii) Derivatives With DNA[J]. Biochem.Biophys. Res. Commun., 1983,113(1):148-162.
[132] Nakamura J., Igarashi S., Highly sensitive spectro- fluorometric determination of nucleic acid based on the aggregation of two oppositely charged kinds of water-soluble porphyrins[J]. Anal.Lett., 1996, 29(14): 2453-2461.
[133] Li, Wen-You, Xu, Jin-Gou, He, Xi-Wen. Determination of nucleic acids using rivanol as the fluorescent probe in the presence of SDS[J]. Analytical Letters, 2000, 33 (15): 3183-3194
[134] 贺吉香，江崇球，高明霞．盐酸小檗碱与脱氧核糖核酸相互作用的研究[J]．光谱学与光谱分析，2003，(4)：132-135
[135] 胡敏，杨欣，晋卫军，曹祉祥，沈国励，俞汝勤．荧光光谱法研究抗癌新药吡柔比星与DNA的相互作用[J]．药学学报，1999，34(8)：608-612
[136] Wen-You Li, Jin-Gou Xu, Xiang-Qun Guo, Qing-zhi Zhu, Yi-Bing Zhao. Application of vitamin kappa-3 as a photochemical fluorescence probe in the determination of nucleic acids[J]. Anal. Lett. 1997, 30(2), 245-257
[137] Wenyou Li,Xiangqun Guo, Jingou Xu, Qingzhi Zhu, Yibing Zhao. Determination of DNA using sodium 9,10-anthraquinone-2-sulfonate as an in situ photochemical fluorescence probe[J]. Anal. Chim. Acta, 1997, 340 (1-3) :291-296
[138] Yun-Xiang Ci, Yuan-Zong Li, and Xiao-Jing Liu, Selective Determination of DNA by its Enhancement Effect on the Fluorescence of the Eu-Tetracycline Complex[J]. Anal. Chem. 1995, 67(11): 1785-1788
[139] Yi-Bing Zhao, Dong-Yun Wang, Xiang Qun Guo, Jin Guo Xu. Determination of DNA and RNA by their quenching effect on the fluorescence of the Tb3+-Tiron complex[J]. Anal. Chim. Acta, 1997, 53(2-3): 329-335
[140] Jing Zhou, Guo-quan Gong, Ying-Nian Zhang, Jian-Qiang Qu, Liu-Fang Wang,Ji-Wu Xu, Quercetin-La(Ⅲ) complex for the fluorimetric determination of nucleic acids[J]. Anal. Chim. Acta,1999,38 I(1):17-22
[141] C. Z. Huang, K. A. Li, S. Y. Tong, Fluorescent Complexes of Nucleic Acids-8-Hydroxyquinoline-La(Ⅲ) and The Spectrofluorimetry of Nucleic Acids[J]. Anal. Lett., 1996, 29(10): 1705-1717
[142] Xia Wu, Jinghe Yang, Fang Huang, Min Wang, Limei Sun, Guiying Xu, Study of the reaction between nucleic acid and Tb-BPMPHD-CTMAB complex and its analytical application[J]. Anal. Lett., 1999,32(12): 2417-2425
[143] G. L. Wang, J. L. Yuan, K. Matsumoto, Z. Hu, Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA- Eu(III)-beta-diketonate ternary complex[J]. Anal. Biochem., 2001, 299(2): 169-172
[144] C. G. Lin, G. L. Zhang, J. H. Yang, Fluorimetric determination of nucleic acids with terbium and lutetium[J]. Microchem. J., 2002, 71(1): 9-14
[145] Bernard Juskowiak,Ilona Grzybowska, Elzbieta Galezowska,Shigeori Takenaka. Enhanced fluorescence of the Eu3+-naphthalenediimide derivative-phenanthroline ternary complex and the determination of DNA[J]. Anal. Chim. Acta,2004,512(1):133-139
[146] Wu X, Guo C, Yang J, Wang M, Chen Y, Liu J. The sensitive determination of nucleic acids using fluorescence enhancement of Eu3+-benzoyl acetonecetyltrimethylammonium bromide- nucleic acid system[J]. J Fluoresc. 2005,15(5):655-660.
[147] Yegorova A, Karasyov A, Duerkop A, Ukrainets I, Antonovich V. New luminescent terbium complex for the determination of DNA[J]. Spectrochim Acta A. 2005, 61(1-2):109-116.
[148] Zhu CQ, Zhuo SJ, Chen JL, Wu YQ, Li YX, Li DH, Zheng H, Xu JG A kinetic fluorometric method for the determination of nucleic acids using a ternary equilibrium system of nucleic acids - iron(III) tetracarboxy phthalocyanine - poly-lysine coupled with the oxidation reaction between hydrogen peroxide and DL-tyrosine[J]. Analytica Chimica Acta, 2004, 514 (2): 247-252
[149] Qiu-E Cao, Yunkun Zhao,Yunyuan Xu, Changzheng Li, Zhide Hu, and Qiheng Xu. Studies on the Fluorescence Reaction between Nucleic Acid and the Complex of Cobalt(II) with 5-(3-Fluoro-4-chlorophenylazo)-8-sulfonamidoquinoline and its Applications[J]. Analytical Biochemistry, 2000,277(2):214-220.
[150] Ling LS, He ZK, Song GW, Zeng YE, Wang C, Bai CL, Chen XD, Shen P. High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)2(dppx)2+ [J]. Analytica Chimica Acta, 2001,436 (2): 207-214.
[151] Lian-Sheng Ling, Zhi-Ke He, Gong-Wu Song, Din Yuan, Yun-e Zeng. A novel method for determination of DNA by use of molecular "Light Switch" complex of Ru(bipy)2(dppx)2+ [J]. Analytica Chimica Acta, 2000,403 (1-2): 209-217
[152] 朱展才，汪静，朱红斌．铌．桑色素荧光探针猝灭法测定核酸[J]．分析化学，2002，30(11)：1319-1321
[153] 龙朝阳，俞英，吴建中，孙姒巧．铜(Ⅱ)．邻菲咯啉-(邻菲咯啉-5，6-二酮)络合物的合成及其同脱氧核糖核酸的作用[J]．分析化学，2002，30(4)：392-394
[154] 王岩玲，杨周生．联吡啶一铜(Ⅱ)络合物荧光增强法测定核酸[J]．分析化学，2005，33(1)，57-59
[155] Yun-Xiang Ci,Yong Chen,Yuan-Zong Li,Wen-Bao Chang. The Use of Mn-TPPS4 Mimetic Peroxidase in a DNA Hybridization Assay[J]. Microchemical Journal, 1995,52(3): 257-262.
[156] 张延荣，宗志新，龚国权，孙彬，段海峰，穆德琪，徐喜莲．核酸-槲皮素-锌(Ⅱ)三元配合物荧光法测定核酸[J]．兰州大学学报(自然科学版)，2000，36(3)：100-104
[157] J. H. Yang, C. G. Lin, G. L. Zhang, Z.Q.Gao, X.Wu, X.R. Huang,Study on a fluorescence enhanced system of 1, 2, 4-trihydroxyanthro quinone-Be2+-DNA[J]. Spectrochim. Acta.A, 1998, 54(12): 2019-2024
[158] 林旭聪，谢增鸿，郭良洽，陈国南，核酸-桑色素·铝(Ⅲ)三元荧光体系的研究[J]．光谱学与光谱分析，2004，24(10)，1230-1234
[159] D. H. Li, X. L. Chen, Y. Fang, J. G. Xu, Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and acridine orange[J]. Analyst, 2001, 126(4): 518-522
[160] C. Z. Huang, Y. F. Li, S. Y. Tong, Spectrofluorimetry of Nucleic Acids on the Their Ternary Complexes of Aluminum(Ⅲ)/8-Hydroxyquinoline[J]. Anal. Lett., 1997, 30(7): 1305-1309
[161] 林旭聪，谢增鸿，郭良洽，林坚，陈国南．铟(Ⅲ)一8一羟基喹啉一核酸三元荧光体系的研究与应用[J]．光谱实验室，2003，20(6)810-814
[162] J. Lagona, P. Mukhopadhyay, S. Chakrabarti, L. Isaacs. The cucurbit[n]uril family[J]. Angew. Chem. Int. Ed. 2005,44(31):4844-4670
[163] R. Hoffmann, W. Knoche, C. Fenn, H.-J. Buschmann. Host-guest Complexes of Cucurbituril with the 4-Methylbenzylammonium Ion, Alkali-metal Cations and NH4+[J]. J. Chem. Soc. Faraday Trans. 1994,90 (11) 1507-1511.
[164] 李刚，冯亚青．葫芦脲[J]．化学通报，2005，68，w054(http://www.hxtb.org)
[165] W. A. Freeman, W. L. Mock, N. Y. Shih. Cucurbituril [J]. J.Am.Chem. Soc. 1981, 103(24): 7367-7368
[166] H.J. Buschmann, E. Cleve and E. Schollmeyer.Cucurbituril as a ligand for the complexation of cations in aqueous solutions[J]. Inorg. Chim. Acta. 1992,193(1):93-97
[167] H.-J. Buschrnann, E. Cleve, K. Jansen, A. Wego, E. Schollmeyer. Complex Formation between Cucurbit[n]urils and Alkali, Alkaline Earth and Ammonium Ions in Aqueous Solution[J]. J. Incusion. Phenom. Mol. Recognit. Chem. 2001,40(1-2), 117-120
[168] H. -J. Buschmann, E. Cleve, K. Jansen and E. Schollmeyer. Determination of complex stabilities with nearly insoluble host molecules: cucurbit[5]uril, decamethylcucurbit[5]uril and cucurbit[6]uril as ligands for the complexation of some multicharged cations in aqueous solution [J]. Anal.Chim.Acta 2001,437(1): 157-163
[169] W. L. Mock and N. Y. Shih. Host-guest binding capacity of eucurbituril[J]. J. Org. Chem., 1983,48(20):3618-3619
[170] William L. Mock and Neng Yang Shih. Structure and selectivity in host-guest complexes of cucurbituril[J]. J. Org. Chem.,1986,51 (20): 4440-4446.
[171] William L. Mock and Neng-Yang Shih. Organic Ligand-Receptor Interactions between Cucurbituril and Alkylammonium Ionstyl[J]. J. Am. Chem. Soc., 1988, 110(14), 4706-4710
[172] H.J. Buschmann, K. Jansen, E. Schollmeyer The formation of cucurbit[6]uril complexes with amino acids and amino alcohols in aqueous formic acid studied by calorimetric titrations[J]. Thermochimica Acta, 1998,317(1):95-98
[173] Buschmann H J, Schollmeyer E. Cucurbituril and β-cyclodextrin as hosts for the complexation of organic dyes[J]. J. Incl. Phenom. Mol. Reconit. Chem. 1997, 29:167-174
[174] Roland Neugebauer and Wilhelm Knoche. Host-guest complexes of cucurbituril with 4-amino-4'-nitroazobenzene and 4'-diaminoazo benzene in acidic aqueous solutions [J]. J. Chem. Soc., Perkin Trans.2 1998,3:529-534
[175] 杨守林，薛赛凤，马培华，祝黔江，陶朱，张建新．瓜环与4，4’-二氨基二苯甲烷衍生物的相互作用[J]．2005，25(1 0)：1215～1220
[176] 杜莹，薛赛凤，祝黔江，陶朱．紫外吸收光谱法测定瓜环与喹啉衍生物包结物包结比[J]．贵州大学学报(自然科学版)，2005，22(2)：165-168
[177] H-J Buschmann, T. Wolff, Fluorescence of 1-anilino naphthalene-8-sulfonate in solid macrocyclic environments[J]. Journal of Photochemistry and Photobiology A, 1999,121 (2): 99-103
[178] Brian D. Wagner and Andrew I. MacRae. The Lattice Inclusion Compound of 1,8-ANS and Cucurbituril: A Unique Fluorescent Solid[J]. J. Phys. Chem. B 1999, 103(46): 10114-10119
[179] Brian D. Wagner, Shannon J. Fitzpatrick, Monica A. Gill, Andrew I. MacRae, and Natasa Stojanovic. A fluorescent host-guest complex of cucurbituril in solution: a molecular Jack O'Lantern[J]. Can. J. Chem./Rev. Can. Chim. 2001,79(7): 1101-1104
[180] Brian D. Wagner,Natasa Stojanovic, Anthony I. Day, Rodney J. Blanch. Host Properties of Cucurbit[7]uril: Fluorescence Enhancement of Anilinonaphthalene Sulfonates[J]. J. Phys. Chem. B 2003, 107(39): 10741-10746
[181] Rankin, MA, Wagner, BD. Fluorescence enhancement of curcumin upon inclusion into cucurbituril[J]. Supramol Chem. 2004,16 (7): 513-519
[182] Sung Im Jun, Jae Wook Lee, Shigeru Sakamoto, Kentaro Yamaguchi and Kimoon Kim. Rotaxane-based molecular switch with fluorescence signaling[J]. Tetrahedron Letters, 2000, 41 (4): 471-475
[183] Mohanty, J, Nau, WM. Ultrastable rhodamine with cucurbituril[J]. Angew. Chem. Int. Edit. 2005, 44 (24): 3750-3754
[184] Nau, WM, Mohanty, J. Taming fluorescent dyes with cucurbituril[J]. Int J Photoenergy, 2005,7 (3): 133-141
[185] Jyotirmayee Mohanty and Werner M. Nau. Refractive index effects on the oscillator strength and radiative decay rate of 2,3-diazabicyclo[2.2.2] oct-2-ene[J]. Photochemical & Photobiological Sciences, 2004, 3(11-12): 1026-1031
[186] C. Marquez, F. Huang, W. M. Nau. Cucurbiturils: molecular nanocapsules for time-resolved fluorescence-based assays [J]. IEEE Trans. Nanobiosci. 2004,3 (1): 39-45
[187] C. Marquez, W. M. Nau. Polarizabilities Inside Molecular Containers [J]. Angew. Chem. Int. Ed. 2001, 40(23): 4387-4390.
[188] Lim YB, Kim T, Lee JW, Kim SM, Kim HJ, Kim K, Park JS. Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: noncovalent strategy in developing a gene delivery carrier[J]. Bioconjug Chem. 2002, 13(6):1181-1185.
[189] Rosen C G, Weber G. Dimer formation from 1-anlino-8-naphthlaene-sulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with exceptional binding properties[J]. Biochemistry,1969, 8(10):3915-3920
[190] 刘思敏 严砚 吴成泰 葫[n]环联研究进展．大环化学和超分子化学研究进2002．9(西安)
[191] H.J. Buschmann, E. Schollmeyer, L. Mutih. The formation of amino acid and dipeptide complexes with a-cyclodextrin and cucurbit[6]uril in aqueous solutions studied by titration calorimetry [J]. Thermochimica Acta 2003,399(l-2):203-208
[192] Brian D. Wagner and Shannon J. Fitzpatrick. A Comparison of the Host-Guest Inclusion Complexes of 1,8-ANS and 2,6-ANS in Parent and Modified Cyclodextrins [J]. J. Incl. Phenom. Macro. Chem. 2000, 38(1-4):467-478.
[193]X.L.Xu. Experiment of biochemistry [M].Beijing:Medical Technology of China Press, 1994,p31
[194] E. Gaggelli, F. Berti, N. D'Amelio, N. Gaggelli, G. Valensin, L. Bovalini, A. Paffetti, L. Trabalzini. Metabolic pathways of carcinogenic chromium, environmental health perspectives [J]. 2002, 110: 733-738.
[195] S. Caspi, E. Ben-Jacob. Conformation changes and folding of proteins mediated by Davydov's soliton [J]. Physics Letters A, 2000, 272: 124-129.
[196] W.S. Tao. Protein Macular Basic [M]. Beijing: The People's Education Press, 1981, p256.
[197] Patra, Digambara. Mishra.A.K.pp. Recent developments in multi-component synchronous fluorescence scan analysis[J]. TrAC Trends in Analytical chemistry.2002, 21(12):787-798
[198]Xiujuan Yang, Ju Chou, Gongquan Sun, Hui Yang and Tianhong Lu Synchronous Fluorescence Spectra of Hemoglobin: A Study of Aggregation States in Aqueous Solutions[J]. Microchemical J. 1998,60(3):210-216
[199] 孙长侠 博士学位论文 2005, p98
[200] T.D. Brown, T.M. Shepherd. Efficiencies of fluorescent Tb(III) chelates in the solid state [J]. J. Chem. Soc: Dalton Trans., 1973, 3: 336-341.
[201] Wu P.G, Brand L. Resonance Energy Transfer: Methods and Applications [J]. Anal.Biochem.,1994, 218(1):1-13
[202] R.M.Clegg. Fluorescence resonance energy transfer[J].Curr.Opin.In. Biotech, 1995,6(1):103-110
[203] G.A.Kumar,N.V. Unnikrishnan. Energy transfer and optical gain studies of FDS: RhB dye mixture investigated under cw laser excitation [J]. J. Photochem. Photobiol. A, 2001,144(2-3): 107-117
[204] 马春琪 李克安 赵凤林 童沈阳．牛血清白蛋白与铬天青S作用机理的研究[J]．化学学报1999．57(4)：389-395
[205] Denis G. Samsonenko, Janusz Lipkowski, Olga A. Gerasko, Alexander V. Virovets, Maxim N. Sokolov, Vladimir P. Fedin, Javier G. Platas, Rita Hernandez-Molina,and Alfredo Mederos. Cucurbituril as a New Macrocyclic Ligand for Complexation of .Lanthanide Cations in Aqueous Solutions [J]. Eur. J. Inorg. Chem. 2002, 2002(9):2380-2388
[206] 罗绪强，张桂玲，薛赛凤，陶朱，祝黔江．酸度及碱金属离子在瓜环合成分离中的应用研究[J]．贵州大学学报(自然科学版)2003，20(3)，303-305
[207] 沈星灿 何锡文 梁宏 纳米粒子特性与生物分析[J]．分析化学2003，31(7)，880-885
[208] 沈星灿，袁琦，梁宏，闫海刚，何锡文．血清白蛋白与银纳米粒子相互作用的滞后效应[J]．中国科学B辑，2002，33(2)，177-184
[209] 郭德华，王敏，李芳．高效液相色谱法测定水产品中的噁喹酸[J]．化学分析计量 2003，12(1)：24-25
[210] A. E. Tyrpenou, , G. Rigos Determination of Oxolinic Acid Residues in Gilthead Seabream (Sparus aurata) Muscle Tissue and Plasma by High-Performance Liquid Chromatography[J]. Chromatographia 2004,60 (11/12):657-661
[211] 陈国珍等 荧光分析法(第二版) 科学出版社 1990，p115
[212] Fierens, C.; Hillaert, S.; and Bossche W. V. The qualitative and quantitative determination of quinolones of first and second generation by capillary electrophoresis[J]. Journal of Pharmaceutical and Biomedical Analysis, 2000, 22(5), 763-772
[213] Cohen, E.; Maxwell, R. J.; and Donoghue, D. J. Automated multi-residue isolation of fluoroquinolone antimicrobials from fortified and incurred chicken liver using on-line microdialysis and high-performance liquid chromatography with programmable fluorescence detection[J]. Journal of Chromatography B, 1999, 724(1):137-145
[214] Yorke, J.C. and Froc, P. Quantitation of nine quinolones in chicken tissues by highperformance liquid chromatography with fluorescence detection[J]. J. Chromatogr. A, 2000, 882 (1-2): 63-77.
[215] Pouliquen, H. and Armand, F. Determination of oxolinic acid in faeces and urine of turbot (Scophthalmus maximus) by high-performance liquid chromatography using fluorescence detection[J]. Journal of Chromatography B, 2000, 749(1): 127-133
[216] Tyrpenou, A. E.; Rigos, G. Determination of Oxolinic Acid Residues in Gilthead Seabream (Sparus aurata) Muscle Tissue and Plasma by High-Performance Liquid Chromatography [J]. Chromatographia, 2004, 60(11-12): 657-661
[217] Loussouarn, S.; Pouliquen, H. and F. Armand. High-performance liquid chromatographic determination of oxolinic acid in the plasma of seabass (Dicentrarchus labrax) anaesthetized with 2-phenoxyethano[J]. J. Chromatogr. B 1997, 698(1-2): 251-259.
[218] Touraki, M.; Ladoukakis, M.; Prokopiou, C. High-performance liquid chromatographic determination of oxolinic acid and flumequine in the live fish feed Artemia [J]. Journal of Chromatography B, 2001,751 (2), 247-256
[219] Dorota, K. and Hanna, H. Application of derivative UV spectrophotometry for the determination of cinoxacin and oxolinic acid in pure and dosage form [J]. Chem.Anal. (Warsaw), 2003, 48 (1): 97-106.
[220] Liu, J.Q., Wang, X., and Zhao, H.C. Europium sensitized fluorescence assay of rufloxacin in human serum and urine [J]. Chinese Journal of Analysis Laboratory, 2001, 20 (4): 79-82.
[221] Tong, C.L., Xiang, G.H., Huang, D.J., and Liu, W.P. Determination of ofloxacin by the terbium(III) ion fluorescence probe sensitized by the surfactant[J]. Chinese Journal of Analytical Chemistry, 2004, 32 (5): 619-621.
[222] Wu, S.Q., Cao, Q.E., Zhao, Y.K., Zhang, W.J., Chen, X.G., and Hu, Z.D. The fluorescence characteristic of Tb-ciprofloxacin and the determination of ciprofloxacin[J]. Chinese Journal of Analytical Chemistry, 2000,28 (12): 1462-1466.
[223] 王静萍，杜黎明，段亚丽．铽．依诺沙星荧光特性研究及应用[J]．分析化学，2004，32(1)：50-52
[224] 潘建章，李树伟，何艺，苏小东，王小红．铽．司帕沙星荧光体系的研究及应用[J]．四川师范大学学报(自然科学版)，2005，28(1)：101-103
[225] 戚雪勇，何华，顾飞，余江河．铽离子增敏环丙沙星研究及其应用[J]．中国药学杂志，2005，40(19)：64-67
[226] 周静，杜黎明，陈彩萍．铽-培氟沙星的荧光特性研究及培氟沙星的测定[J]．光谱学与光谱分析．2004，24(6)：78-80
[227] Beltyukova S.V.; Egorova A.V. Terbium chelates for fluorescence immunoassays[J]. J. Pharm. Biomed. Anal., 1998,18 (1-2): 267-270.
[228] 李元宗，常文保．生化分析[M]．高等教育出版社，2003，pp146
[229] The Public Health Department of People's Republic of China. Pharmacopoeia of the People's Republic of China[M]. Beijing: Chemical Industry Press, 1995(1): 311-312.
[230] W. Q. Sun, J. F. Sheng. Handbook of Natural Active Constituents[M]. Beijing: Chinese Science and Technology Press, 1998, pp2240-2316.
[231] Y.X. Peng, W.H. Zhu, J.H. Chen, Experimental Biochemistry[M]. The People's Education Press, 1989, pp. 79-80.
[232] S.J. Qin, J. S. Shen,Y. Y. Li, Y. J. Wei. Study on combination of the rare-earth metal ions and bovine serum albumin[J]. Journal of Hebei normal university(Natural Science Edition), 2003, 27(3): 274-277.
[233] Xiao-jing Liu, Yuan-zong Li, Yun-xiang Ci. Time-resolved fluorescence studies of the interaction of the Eu3+ complexes of tetracycline analogues with DNA[J]. Anal. Chim. Acta, 1997, 345(1-3): 213-217.
[234] 朱庆枝，李芳，郭详群，许金钩，李文友．竹红菌素A与DNA作用的研究[M]．分析化学新进展(山西科技出版社)，1997，pp244-245
[235] A. M. Pyle, J. P. Rehmann, R. Meshoyrer, C. V. Kumar, N. J. Turro, Jacqueline K. Barton. Mixed-ligand complexes of ruthenium(II): factors governing binding to DNA [J]. J. Am. Chem. Soc, 1989, 111(8): 3051-3058.
[236] A. Nakajima. A study on the system of pyrene and β-cyclodextrin in aqueous solution utilizing the intensity enhancement phenomenon[J]. Spectrochim. Acta. A, 1983, 39(10): 913-915.
[237] Y. C. Jiang, S. K. Wu. A study on the micellar viscosity in surfactant solution by fluorescence polarization[J]. Photographic science and photochemistry, 1995, 13(2): 180-185.