玻璃体切割术后微环境的改变对晶状体纤维细胞DLAD基因表达的影响
摘要
目的:观察兔眼玻璃体切割术后玻璃体微环境的改变对晶状体纤维细胞脱氧核糖核酸酶DLAD基因表达的影响,阐述玻璃体切割术后并发性白内障的发生机制。
方法:通过对玻璃体切割术后实验动物模型在玻璃体微环境改变所引起的眼压、炎性因子和氧浓度等变化的检测,运用半定量RT-PCR技术检测DLAD基因在玻璃体切割术后晶状体纤维细胞上的表达,从分子水平探讨并发性白内障的发生机制。
成果:平衡盐组、气体组和硅油组在术后1天、3天、7天、1月、3月等5个时间点的表达强度不一致,随时间改变DLAD mRNA的表达逐渐减弱;同一时间点三组的DLAD mRNA的表达水平不一致,其中以硅油组下降明显。平衡盐组、气体组和硅油组等三组实验眼DLAD基因的表达量均低于对照眼,差异有统计学意义(P<0.05)。
结论:玻璃体切割术后微环境改变引起晶状体纤维细胞DLAD基因的表达减少,DLAD基因的低表达可能在玻璃体切割术后并发性白内障的形成中起重要作用。
Objective: To explore the mechanism of cataract formation through the study of DLAD (DNaseⅡ-like acid DNase) gene expression in rabbits lens after vitrectomy.
Methods: Detecting the variation of intraocular pressure, inflammatory factor and oxygen concentration, and applying semiquantitative RT-PCR to detect DLAD gene expression in fibrocyte of rabbits lens after vitrectomy.
Results: DLAD gene expression was all decreased in the exprement.
Conclusions: The low expression of DLAD gene may play a important role in nuclear cataract formation after vitrectomy.
方法:通过对玻璃体切割术后实验动物模型在玻璃体微环境改变所引起的眼压、炎性因子和氧浓度等变化的检测,运用半定量RT-PCR技术检测DLAD基因在玻璃体切割术后晶状体纤维细胞上的表达,从分子水平探讨并发性白内障的发生机制。
成果:平衡盐组、气体组和硅油组在术后1天、3天、7天、1月、3月等5个时间点的表达强度不一致,随时间改变DLAD mRNA的表达逐渐减弱;同一时间点三组的DLAD mRNA的表达水平不一致,其中以硅油组下降明显。平衡盐组、气体组和硅油组等三组实验眼DLAD基因的表达量均低于对照眼,差异有统计学意义(P<0.05)。
结论:玻璃体切割术后微环境改变引起晶状体纤维细胞DLAD基因的表达减少,DLAD基因的低表达可能在玻璃体切割术后并发性白内障的形成中起重要作用。
Objective: To explore the mechanism of cataract formation through the study of DLAD (DNaseⅡ-like acid DNase) gene expression in rabbits lens after vitrectomy.
Methods: Detecting the variation of intraocular pressure, inflammatory factor and oxygen concentration, and applying semiquantitative RT-PCR to detect DLAD gene expression in fibrocyte of rabbits lens after vitrectomy.
Results: DLAD gene expression was all decreased in the exprement.
Conclusions: The low expression of DLAD gene may play a important role in nuclear cataract formation after vitrectomy.
引文
[1] Chen L,Azen SP,El-Bradey MH,et al.Duraion of vitrectomy and Postoperative catatract in the vitrectomy for macular hole study. Am J Ophthalmol,2001,132(6);881-887.
[2]周伟,李学喜.眼内硅油填充并发性白内障手术治疗.国际眼科杂志2008;8(2): 345-347.
[3] A yaki M,Ohoguro N,A zuma N,et al.Detection of cytotoxic antiLEDGF auto antibodies in a topic dermatitis[J].Autoimmunity, 2002;35 (5):319-27.
[4]梁建宏,郑雷,Irene Barbazetto等.玻璃体切割术对晶状体氧分压的影响.眼科学报,2002,18(2):67-70.
[5] Cherfan GM, Michels RG de Bustros S, et al .Nuclear selerotic cataract after vitrectomy for idiopathice piretinal membranes causing macular pueker.Am J Ophthalmol 1991,111:434-438.
[6] Bettelheim FA, Lizak MJ, Zigler JS.Syneretic response of aging normal human lens to pressure[J].Invest Ophthalmol Vis Sci,2003;44(1):258-63.
[7] Kuszak JR, Sivak JG, Herbert KL, et al. The relationship between rabbit lens optical quality and sutural anatomy after vitrectomy. Exp Eye Res, 2000,71:267-281.
[8]毛宝龄,王本清.临床血气分析.北京:人民军医出版社,1985.1.
[9]时杰,王秋,李学武.针刺对实验性兔眼环扎术后房水氧含量的影响.北京中医药大学学报,2000,23(4):52-53.
[10] H suan JD,Brown NA,Bron AJ,et al.Posterior subcapsular and nuclear cataract after vitrectomy [J] Cataract Refract Surg,2001;27(3):437-44.
[11] McNulty R, Wang H, Mathias RT, et al. Regulation of tissue oxygen levels in the mammalian lens. J Physiol 2004, 559:883–898.
[12] Barbazetto IA, Liang J, Chang S, et al. Oxygen tension in the rabbit lens and vitreous before and after vitrectomy. Exp Eye Res, 2004, 78:917–924.
[13] Maeda N, Tano Y. Intraocular oxygen tension in eyes with proliferative diabetic retinopathy with and without vitreous. Graefes Arch Clin Exp Ophthalmol, 1996, 234(suppl 1):S66–S69.
[14] Helbig H, Hinz JP, Kellner U, Foerster MH. Oxygen in the anterior chamber of the human eye. Ger J Ophthalmo, 1993, 2:161–164.
[15] Harocopos GJ, Shui YB, McKinnon M, et al. Importance of vitreous liquefaction in age-related cataract. Invest Ophthalmol Vis Sci,2004,45: 77-85.
[16] Schriner SE, Linford NJ, Martin GM, et al. Extension of murine lifespan by overexpression of catalase targeted to mitochondria. Science, 2005:308:1909–1911.
[17] Marsili S, Salganik RI, Albright CD, et al. Cataract formation in a train of rats selected for high oxidative stress. Exp Eye Res,2004,79:595–612.
[18] Barbazetto IA, Liang JH, Stanley C. Oxygen tension in the rabbit lens and vitreous before and after vitrectomy. Exp Eye Res, 2004, 78(5):917-924.
[19] Tatatsugu Taniguchi ,yasuhiro Minami ,李翠玲;白细胞素2/白细胞介素2受体系统的新进展[J];国际免疫学杂志; 1993年06期; 48-50.
[20] Fleisher LN, Ferrell JB, McGahan MC Synergistic uveitic effects of tumor necrosis factor-alpha and interleukin-1 beta[J]. Invest Ophthalmol Vis Sci. 1992, 33: 2120-2127.
[21] Nishimoto S, Kawane K, Watanabe-Fukunaga R, et al. Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens. Nature, 2003, 424: 1071-1074.
[22] Shiokawa D, Tanuma S. DLAD, a novel ammalian divalent cation-independent endonuclease with homology to DNase II. Nucleic Acids Res, 1999, 27:4083– 4089.
[23] Krieser RJ, Maclea KS, Park JP, et al. The cloning genomic structure, localization and expression of human deoxyribonucleaseⅡ-beta. Gene, 2001, 269:205-216.
[24] Nagai N, Takeuchi N, Kamei A, Ito Y. Involvement of DNaseⅡ-like acid DNase in the cataract formation of the UPL rat and the Shumiya cataract rat. Biol Pharm Bull, 2006, 29(12):2367-2371.
[25] De Maria A, Bassnett S. DNase IIbeta distribution and activity in the mouse lens. Invest Ophthalmol Vis Sci, 2007, 48(12):5638-5646.
[26] Counis MF, Chaudun E, Arruti C, et al. Analysis of nuclear degradation during lens cell differentiation. Cell Death Differ, 1998, 5(4):251-61.
[2]周伟,李学喜.眼内硅油填充并发性白内障手术治疗.国际眼科杂志2008;8(2): 345-347.
[3] A yaki M,Ohoguro N,A zuma N,et al.Detection of cytotoxic antiLEDGF auto antibodies in a topic dermatitis[J].Autoimmunity, 2002;35 (5):319-27.
[4]梁建宏,郑雷,Irene Barbazetto等.玻璃体切割术对晶状体氧分压的影响.眼科学报,2002,18(2):67-70.
[5] Cherfan GM, Michels RG de Bustros S, et al .Nuclear selerotic cataract after vitrectomy for idiopathice piretinal membranes causing macular pueker.Am J Ophthalmol 1991,111:434-438.
[6] Bettelheim FA, Lizak MJ, Zigler JS.Syneretic response of aging normal human lens to pressure[J].Invest Ophthalmol Vis Sci,2003;44(1):258-63.
[7] Kuszak JR, Sivak JG, Herbert KL, et al. The relationship between rabbit lens optical quality and sutural anatomy after vitrectomy. Exp Eye Res, 2000,71:267-281.
[8]毛宝龄,王本清.临床血气分析.北京:人民军医出版社,1985.1.
[9]时杰,王秋,李学武.针刺对实验性兔眼环扎术后房水氧含量的影响.北京中医药大学学报,2000,23(4):52-53.
[10] H suan JD,Brown NA,Bron AJ,et al.Posterior subcapsular and nuclear cataract after vitrectomy [J] Cataract Refract Surg,2001;27(3):437-44.
[11] McNulty R, Wang H, Mathias RT, et al. Regulation of tissue oxygen levels in the mammalian lens. J Physiol 2004, 559:883–898.
[12] Barbazetto IA, Liang J, Chang S, et al. Oxygen tension in the rabbit lens and vitreous before and after vitrectomy. Exp Eye Res, 2004, 78:917–924.
[13] Maeda N, Tano Y. Intraocular oxygen tension in eyes with proliferative diabetic retinopathy with and without vitreous. Graefes Arch Clin Exp Ophthalmol, 1996, 234(suppl 1):S66–S69.
[14] Helbig H, Hinz JP, Kellner U, Foerster MH. Oxygen in the anterior chamber of the human eye. Ger J Ophthalmo, 1993, 2:161–164.
[15] Harocopos GJ, Shui YB, McKinnon M, et al. Importance of vitreous liquefaction in age-related cataract. Invest Ophthalmol Vis Sci,2004,45: 77-85.
[16] Schriner SE, Linford NJ, Martin GM, et al. Extension of murine lifespan by overexpression of catalase targeted to mitochondria. Science, 2005:308:1909–1911.
[17] Marsili S, Salganik RI, Albright CD, et al. Cataract formation in a train of rats selected for high oxidative stress. Exp Eye Res,2004,79:595–612.
[18] Barbazetto IA, Liang JH, Stanley C. Oxygen tension in the rabbit lens and vitreous before and after vitrectomy. Exp Eye Res, 2004, 78(5):917-924.
[19] Tatatsugu Taniguchi ,yasuhiro Minami ,李翠玲;白细胞素2/白细胞介素2受体系统的新进展[J];国际免疫学杂志; 1993年06期; 48-50.
[20] Fleisher LN, Ferrell JB, McGahan MC Synergistic uveitic effects of tumor necrosis factor-alpha and interleukin-1 beta[J]. Invest Ophthalmol Vis Sci. 1992, 33: 2120-2127.
[21] Nishimoto S, Kawane K, Watanabe-Fukunaga R, et al. Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens. Nature, 2003, 424: 1071-1074.
[22] Shiokawa D, Tanuma S. DLAD, a novel ammalian divalent cation-independent endonuclease with homology to DNase II. Nucleic Acids Res, 1999, 27:4083– 4089.
[23] Krieser RJ, Maclea KS, Park JP, et al. The cloning genomic structure, localization and expression of human deoxyribonucleaseⅡ-beta. Gene, 2001, 269:205-216.
[24] Nagai N, Takeuchi N, Kamei A, Ito Y. Involvement of DNaseⅡ-like acid DNase in the cataract formation of the UPL rat and the Shumiya cataract rat. Biol Pharm Bull, 2006, 29(12):2367-2371.
[25] De Maria A, Bassnett S. DNase IIbeta distribution and activity in the mouse lens. Invest Ophthalmol Vis Sci, 2007, 48(12):5638-5646.
[26] Counis MF, Chaudun E, Arruti C, et al. Analysis of nuclear degradation during lens cell differentiation. Cell Death Differ, 1998, 5(4):251-61.