河南省部分小麦品种（系）的醇溶蛋白和高分子量谷蛋白亚基分析及应用
|
摘要
为明确河南省部分小麦品种(系)的子粒醇溶蛋白和高分子量谷蛋白亚基组成及遗传多样性,于2006～2008年在河南大学和河南农业职业学院开展了本项研究。研究采用酸性聚丙烯酰胺凝胶电泳(A-PAGE)和SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,分别对河南省目前种植的35个小麦品种(系)的醇溶蛋白遗传多样性和高分子量谷蛋白亚基组成进行了分析,主要结果如下:
1.河南省部分小麦品种(系)的醇溶蛋白具有遗传多样性对部分小麦品种(系)的子粒进行醇溶蛋白基因位点的特异性检测,分析了不同品种(系)间醇溶蛋白带型的遗传差异。结果从35份材料中共分离出53条带。11份材料具有1BL/1RS易位系标记性带Gli-B11,占供试材料的31.4%。35份材料间的遗传距离在0.0267～0.926之间,平均值为0.5523。基于遗传距离的聚类分析,供试材料在遗传距离0.58水平上聚为3类,表明供试小麦新品种(系)的醇溶蛋白具有遗传多样性。
2.5+12,2﹡等位基因在河南省部分小麦品种(系)中比例较低对部分小麦品种(系)的高分子量谷蛋白亚基组成的分析,结果指出Glu-1位点上有15种等位基因变异,变异类型及分布频率是:Glu-A1位点3种,分别为1(75.0%)、2﹡(8.33%)、N(16.67%);Glu-B1位点7种,分别为7(5.6%)、7+8(36.12%)、7+9(33.3%)、13+16(5.6%)、13+19(2.8%)、14+15(13.9%)、17+18(2.8%);Glu-D1位点5种,分别为2+12(36.1%)、2+11(2.78%)、3+12(8.3%)、5+10(50.0%),5+12(2.78%)。共有18种亚基组合,且有携带5+12,5+10,1,2﹡,7+8,14+15,17+18,13+16等对面包品质有正向作用的优质亚基。结果表明,5+12、2﹡等在河南省部分小麦品种(系)中的比例仍偏低,未来在小麦育种中还应加强对具有优质谷蛋白基因的亲本材料的引进和利用。
3.高分子量谷蛋白亚基可作为品质分类的重要指标之一将不同类型的高分子量谷蛋白亚基与强筋、中筋、弱筋3个品质类型的品质特性进行对照分析,结果表明与亚基品质效应分析所得的结论相吻合。
4.明确了不同品质类型的代表性品种(系)通过对河南省部分小麦品种(系)子粒醇溶蛋白和高分子量谷蛋白亚基组成的研究,郑麦9023、济麦20号、郑麦366为优质强筋品种,偃展4110、许农5号和郑农17号优质中筋品种,郑丰5号和济麦2号为优质弱筋品种。
In order to clarify content of gliadin、HMW-GS and genetic diversity of some wheat cultivars (lines) in Henan Province, experiments were carried out in Henan University and Henan Vocational College of Agriculture from 2006 to 2008. A-PAGE and SDS-PAGE were used to analyse genetic diversity of gliadin and composition of HMW-GS in 35 wheat cultivars (lines) in Henan Province. The main results were as follows:
1. Some wheat cultivars (lines) in Henan Province have genetic diversity of gliadin detected gliadin gene locus specificity of the grain of some wheat cultivars (lines), analyzed the differences of gliadin-based genetic between the different cultivars (lines). The results showed 53 gliadin bands were separated from 35 materials. 11 materials with a marker of 1BL/1RS translocation with allele Gli-Bll, accounted the test materials for 31.4%. The genetic distance (GD) based on gliadin patterns ranged from 0.0267 to 0.926, with a mean value of 0.5523. Cluster analysis based on genetic distance showed that the studied materials can be classified into 3 clusters at the distance of GD 0.58. It was concluded that some wheat cultivar(slines)in Henan province had a rich genetic diversity based on these results.
2. 5+12,2﹡allele of some wheat cultivars (lines) in Henan Province were lower Analyzed composition of HMW-GS 15 HMW-GS of some wheat cultivars (lines), allele’s variation were identified on Glu-1 site. Subunits 1,2﹡and Null at Glu-A1 were found with frequencies of 75.0%,8.33% and 16.67%, respectively; subunits 7,7+8,7+9,13+16,13+19,14+15 and 17+18 at Glu-B1 with frequencies of 5.6%, 36.12%, 33.3%, 5.6%, 2.8%, 13.9% and 2.8%, respectively; and subunits 2+12, 2+11, 3+12, 5+10, 5+12 at Glu-D1 with frequencies of 36.1%, 2.78%, 8.3%, 50.0% and 2.78%, respectively. There were 18 types of good subunits among them, which promoted the quality of wheat-breeding, such as 5+12, 5+10, 1, 2﹡, 7+8, 14+15, 17+18, 13+16. These results showed 5+12, 2﹡were low in some wheat cultivars (lines) in Henan Province. Therefore more excellent parents for wheat-breeding should be introduced and exploited in the future.
3. HMW-GS can be used as one of important indicators of the quality in classification Comparative Analyzed different types of HMW-GS and strong gluten, Medium Gluten, weak gluten quality 3 types of quality characteristics, and the results are similar to the effects of HMW-GS on quality traits.
4. Different types of quality represent of different cultivars (lines) Through the study of the grain gliadin and HMW-GS composition of some wheat cultivars (lines) in Henan Province, Zhengmai 9023, Jimai20, Zhengmai 366 were Strong Gluten Wheat; Yanzhang 4110, Xunong 5, Zhengnong17 were Medium Gluten Wheat; Zhengfeng 5, Jimai 2 were Weak Gluten Wheat.
1.河南省部分小麦品种(系)的醇溶蛋白具有遗传多样性对部分小麦品种(系)的子粒进行醇溶蛋白基因位点的特异性检测,分析了不同品种(系)间醇溶蛋白带型的遗传差异。结果从35份材料中共分离出53条带。11份材料具有1BL/1RS易位系标记性带Gli-B11,占供试材料的31.4%。35份材料间的遗传距离在0.0267～0.926之间,平均值为0.5523。基于遗传距离的聚类分析,供试材料在遗传距离0.58水平上聚为3类,表明供试小麦新品种(系)的醇溶蛋白具有遗传多样性。
2.5+12,2﹡等位基因在河南省部分小麦品种(系)中比例较低对部分小麦品种(系)的高分子量谷蛋白亚基组成的分析,结果指出Glu-1位点上有15种等位基因变异,变异类型及分布频率是:Glu-A1位点3种,分别为1(75.0%)、2﹡(8.33%)、N(16.67%);Glu-B1位点7种,分别为7(5.6%)、7+8(36.12%)、7+9(33.3%)、13+16(5.6%)、13+19(2.8%)、14+15(13.9%)、17+18(2.8%);Glu-D1位点5种,分别为2+12(36.1%)、2+11(2.78%)、3+12(8.3%)、5+10(50.0%),5+12(2.78%)。共有18种亚基组合,且有携带5+12,5+10,1,2﹡,7+8,14+15,17+18,13+16等对面包品质有正向作用的优质亚基。结果表明,5+12、2﹡等在河南省部分小麦品种(系)中的比例仍偏低,未来在小麦育种中还应加强对具有优质谷蛋白基因的亲本材料的引进和利用。
3.高分子量谷蛋白亚基可作为品质分类的重要指标之一将不同类型的高分子量谷蛋白亚基与强筋、中筋、弱筋3个品质类型的品质特性进行对照分析,结果表明与亚基品质效应分析所得的结论相吻合。
4.明确了不同品质类型的代表性品种(系)通过对河南省部分小麦品种(系)子粒醇溶蛋白和高分子量谷蛋白亚基组成的研究,郑麦9023、济麦20号、郑麦366为优质强筋品种,偃展4110、许农5号和郑农17号优质中筋品种,郑丰5号和济麦2号为优质弱筋品种。
In order to clarify content of gliadin、HMW-GS and genetic diversity of some wheat cultivars (lines) in Henan Province, experiments were carried out in Henan University and Henan Vocational College of Agriculture from 2006 to 2008. A-PAGE and SDS-PAGE were used to analyse genetic diversity of gliadin and composition of HMW-GS in 35 wheat cultivars (lines) in Henan Province. The main results were as follows:
1. Some wheat cultivars (lines) in Henan Province have genetic diversity of gliadin detected gliadin gene locus specificity of the grain of some wheat cultivars (lines), analyzed the differences of gliadin-based genetic between the different cultivars (lines). The results showed 53 gliadin bands were separated from 35 materials. 11 materials with a marker of 1BL/1RS translocation with allele Gli-Bll, accounted the test materials for 31.4%. The genetic distance (GD) based on gliadin patterns ranged from 0.0267 to 0.926, with a mean value of 0.5523. Cluster analysis based on genetic distance showed that the studied materials can be classified into 3 clusters at the distance of GD 0.58. It was concluded that some wheat cultivar(slines)in Henan province had a rich genetic diversity based on these results.
2. 5+12,2﹡allele of some wheat cultivars (lines) in Henan Province were lower Analyzed composition of HMW-GS 15 HMW-GS of some wheat cultivars (lines), allele’s variation were identified on Glu-1 site. Subunits 1,2﹡and Null at Glu-A1 were found with frequencies of 75.0%,8.33% and 16.67%, respectively; subunits 7,7+8,7+9,13+16,13+19,14+15 and 17+18 at Glu-B1 with frequencies of 5.6%, 36.12%, 33.3%, 5.6%, 2.8%, 13.9% and 2.8%, respectively; and subunits 2+12, 2+11, 3+12, 5+10, 5+12 at Glu-D1 with frequencies of 36.1%, 2.78%, 8.3%, 50.0% and 2.78%, respectively. There were 18 types of good subunits among them, which promoted the quality of wheat-breeding, such as 5+12, 5+10, 1, 2﹡, 7+8, 14+15, 17+18, 13+16. These results showed 5+12, 2﹡were low in some wheat cultivars (lines) in Henan Province. Therefore more excellent parents for wheat-breeding should be introduced and exploited in the future.
3. HMW-GS can be used as one of important indicators of the quality in classification Comparative Analyzed different types of HMW-GS and strong gluten, Medium Gluten, weak gluten quality 3 types of quality characteristics, and the results are similar to the effects of HMW-GS on quality traits.
4. Different types of quality represent of different cultivars (lines) Through the study of the grain gliadin and HMW-GS composition of some wheat cultivars (lines) in Henan Province, Zhengmai 9023, Jimai20, Zhengmai 366 were Strong Gluten Wheat; Yanzhang 4110, Xunong 5, Zhengnong17 were Medium Gluten Wheat; Zhengfeng 5, Jimai 2 were Weak Gluten Wheat.
引文
[1]姚大年,刘广田,朱金宝,等.小麦品种面粉粘度性状及其与面条品质的相关性研究[J].中国农业大学学报,1997(2):52-68.
[2]李鸿恩.我国小麦种质资源主要品质特性鉴定结果及评价[J].中国农业科学,1995,28(5):29-37.
[3]马传喜,徐风,程国旺.高分子量麦谷蛋白亚基作为面包小麦品种烘烤品质评价指标的研究[J].安徽农业大学学报,1995(2):103-107.
[4]刘东涛,冯国华,谢佩松,等.黄淮麦区小麦品种高分子量谷蛋白亚基组成分析[J].麦类作物学报2007(4):645-648.
[5] D.R马歇克,等著,朱厚础译.蛋白质纯化与鉴定实验指南[M].北京:科学技术出版社,1999.
[6]孙辉,李保云,王岳光,等.普通小麦谷蛋白亚基与烘烤品质的关系[J].中国农业大学学报, 2000(3):118-123.
[7]刘华,王宇生,张辉,等.小麦种质资源醇溶蛋白指纹图谱数据库的初步建立及应用[J].作物学报,1999,25(6):674-682.
[8]曹莉,王辉,李学军.黄淮冬麦区区试小麦品种(系)的品质性状分析及评价[J].麦类作物学报,2001,21(3):68-71.
[9]陆燕,马传喜.小麦品种麦谷蛋白亚基的品质效应研究进展[J].安徽农业大学学报:自然科学版,2000,(2):89-94.
[10]雷东锋,马建岗,赵文明.小麦种子贮藏蛋白质研究进展[J].西北植物学报,2001(3):67-69.
[11]李学军,王辉,李立群,等.高分子量麦谷蛋白亚基的品质效应研究进展[J].干旱地区农业研究,2003(3): 19-22.
[12]刘凤云,黄世全,张大乐,等.河南地区部分小麦新品种高分子量谷蛋白亚基组成分析[J].河南大学学报:自然科学版,2008(1):71-75.
[13]刘凤云,黄世全,张大乐,等.我国部分优质小麦醇溶蛋白遗传多样性分析[J].河南师范大学学报:自然科学版,2008(1):107-110.
[14] Zhang Xueyong,Yang Xueshen,Dong Yuchen.Genetic analysis of germplasm by acid polyacrylamide gel electrophoresis of gliadin[J].Scientia Agricultura Sinica,1995,28(4):25-32.
[15]李玉莲,黄承彦.小麦高分量麦谷蛋白亚基研究进展[J].山东农业科学,2004(5):52-54.
[16] Branlard G.Study of genetic determination of 20 gliadin bands.Theoretical Applied and Geneti-cs,1983(64):155-162.
[17] Metakovsky E V,Knezevich D,Javornik.Gliadin allele composition of Yugoslav winter wheat cultivars.Euphytica,1991(54):85-295.
[18]郎明林,卢少源,赵家发.适合我国小麦醇溶蛋白指纹图谱绘制的改良A-PAGE分子标记技术[J].北农业大学学报,1998(4):1-5.
[19]晏月明,茹岩岩,余建中,等.中国小麦品种醇溶蛋白Gli-1和Gli-2编码位点等位基因组成分析[J].农业生物技术学报,2000,8(1):23-27.
[20]晏月明,刘广田,Prodanovic,等.小麦醇溶蛋白基因的HPCE和A-PAGE染色体定位及其比较分析[J].农业生物技术学报,1998(2):67-71.
[21]魏育明,郑有良,刘登才等.四川小麦地方品种Gli-1、Gli-2和Glu-1位点的遗传多样性[J].植物学报,2000,42(5):496-501.
[22] Woychik J H,Boundy J A,Dimler R J.Starch gel electrophoresis of wheat gluten proteins with concentrated urea.Arch Biochem Biophys,1961(94):477-482.
[23] Gubareva N K,Gavrilyuk I P and Chernoburova A D.Determination of the identity and the cultivar purity of wheat grain according to electrophoretic patterns of the gliadin.Experiment-al methods and catalogue of cultivar electrophoregrams (in Russian).Vaskhnil (Leningrad).1998(67):1-39.
[24] Bushuk W,Zillman R R.Wheat cultivar identification by gliadin electrophoregrams:I. Apparatus,method and nomenclature.Canadian Journal of Plant Science,1978(58):505-515.
[25]颜启传,黄亚军,徐媛.试用ISTA推荐的种子醇溶蛋白电泳方法鉴定大麦和小麦品种[J].1992,18(l):65-67.
[26]阎旭东,卢少源,李宗智.适用于我国的小麦醇溶蛋白分析的A-PAGE方法[J].河北农业大学学报,1994,17(1):7-10.
[27]郎明林,卢少源,赵家发.适合我国小麦醇溶蛋白指纹图谱绘制的改良A-PAGE分子标记技术[J].河北农业大学学报,1998,21(4):1-5.
[28]王学路,钱曼茅,宋春华,等.ISTA醇溶蛋白电泳方法的改进和应用[J].作物遗传资源,1994(2):32-34.
[29]孙立新,张来群,孙海虹,等.小麦醇溶蛋白酸性电泳条件的探索[J].麦类作物学报.1999,25(1):126-129.
[30]邵锦震,丁毅.麦醇溶蛋白A-PAGE方法的优化和改进[J].武汉大学学报(自然科学版),2000(6):733-738.
[31]邵锦震,邱昌恩,丁毅.不同提取剂对麦醇溶蛋白提取效果的比较[J].武汉植物学研究,2003,21(3):262-266.
[32]晏月明,刘广田.小麦醇溶蛋白的遗传与品质改良[J],麦类作物学报,1998,18(l):1-5.
[33]晏月明,刘广田, S Prodanovic等.小麦醇溶蛋白和谷蛋白亚基高校毛细管分离研究[J].中国粮油学报, 1998, 13(4):1-5.
[34]余建中.小麦醇溶蛋白和高分子量谷蛋白亚基高效毛细管电泳研究.首都师范大学,硕士论文,2002.
[35] Devos K M, Bryan G J, Collins A j, et al. Application of two microsatellite sequences in wheat storage proteins as molecular markers[J].Theoretical Applied and Genetics, 1995(90):247-252.
[36] Okita T W,Cheesbrough V,Reeves C D.Evolution and heterogeneity of theα/β-type andγ-type gliadin DNA sequences.J Biol Chem,1985(260):8203-8213.
[37] Lee M. DNA markers and plant breeding programs[J].Adv agron,1995(55):265-344.
[38] Sobko T A, Poperelya F A, Ribalka A I, et al. Inheritance and mapping on chromosome 1A of genes coding for storage proteins in common wheat [J].Tsitol genet, 1986(20):372-376.
[39] Payne PI.Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality[J].Annu Rev Plant Physiol,1987(38):141-153.
[40] Shepherd K W.Genetics of wheat endosperm protein in retrospect and prospect.In Proceeding 7th Genetic Symposium.England Cambridge,1988(6):919-931.
[41] Redaelli R,et al.Two-dimensional mapping of gliadin using biotypes and null mutants of common wheat cultivar Saratovskaya 29.Heredita,1994(121):131-137.
[42] Day P R.The way ahead.Wheat breeding for quality improvement.Chemistry and Physics of Baking.1985(22):251-261.
[43] Mcintosh R A,Hart G E,Gale M D.Catalogue of gene symbols for wheat.Proc 9th Inter Wheat Genet Symp 1998(5):123-127.
[44] Sobko T A,Poperelya F A,Ribalka A I,et al.Inheritance and mapping on chromosome 1A of genes coding for storage proteins in common wheat(in Russian).Tsitol genet,1986(20):372-376.
[45] Metakovsky E V,Akhmadov M G and Sozinov A A.Genetic analysis of gliadin-encoding genes reveals gene clusters as well as single remote genes.Theoretical Applied and Genetics, 1986(73):278-285.
[46] Pogna,N E.Recombination mapping of Gli-5,a new gliadin coding locus on chromosomes 1A and 1B in common wheat.Theoretical Applied and Genetics,1993(87):113-121.
[47] Metakovsky E V,Novoselskaya A Yu,Kopus M M,et al.Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electrophoresis.Theoretica Applied andGenetics.1984(67):559-568.
[48] Metakovsky E V.Gliadin allele identification in common wheat II Catalogue of gliadin alleles in common wheat.Journal Genetics&Breeding,1991(45):325-344.
[49] Metakovsky E V,Brandland G.Genetic diversity of French wheat germplasm based on gliadin alleles.Theoretical Applied and Genetics,1998(96):209-218.
[50]贾继增,张正斌,Devos K,等.小麦21条染色体RFLP作图位点遗传多样性分析[J].中国农业科学,2001(1):13-21.
[51] Metakovsky E V,Annicchiarico P,Boggini G,et al.Relationship between gliadin alleles and dough strength in Italian bread wheat cultivars.Journal of Cereal Science,1997(25):229-236.
[52] Branlard G. Diversity of grain proteins and bread wheat quality I.correlation between gliadin bands and flour quality characteristics[J]. Cultivars J cereal Sci. ,1985(13):329-343.
[53] Payne P I,Kathyrn G C,Linda M H,et al.Correlations between the inheritance of certain high molecular weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat.Journal of the Science of Food and Agriculture,1981(32):51-60.
[54]巩普遍,赵会贤,郭蔼光.小偃6号HMW-GS表达动态研究[J].西北农林科技大学学报:自然科学版,2002(1):65-67.
[55]张学勇,杨欣明,董玉琛.醇溶蛋白电泳在小麦种质资源遗传分析中的应用[J].中国农业科学,1995,25(4):25-32.
[56] Shewry PR, Halford NG, Tatham AS. High molecular weight subunits of wheat glutenin[J]. Journal of Cereal Science, 1992(15):105-120.
[57]黄兴峰,马传喜,司红起,等.小麦品种高分子量谷蛋白亚基的组成分析[J].安徽农业大学学报,2003(4):60-66.
[58] Burnouf T,Bouriquet R.Glutenin subunits of genetically related European hexaploid wheat cultivars:their relation to breadmaking quality.Theoretical Applied and enetics,1980(58):107-111.
[59] Moonen,J H E,Scheepstra A and Graveland A.Use of the SDS-sedimentation test and SDS-polyacrylamide gel electrophoresis for screening breeder’s samples of wheat for bread-making quality.Euphytica,1982(31):677-690.
[60] Lawrence G J and Shepherd K W.Variation in glutenin protein subunits of wheat.Aust.J.Biol. Sci,1980(33):221-233.
[61] Payne P I,Lawrence G J.Catalogue of alleles for the complex gene loci,Glu-A1,Glu-B1 and Glu-D1 which code for high molecular weight subunits of glutenin in hexaploid wheat.Cereal Research Communication,1983(11):29-35.
[62] Ng P K W,Pogna N E,Mellini F et al.Glu-1 allele compositions of the wheat cultivars registered in Canada.J.Genet and Breed.1989(43):53-59.
[63] Anderson O D,Green F C.The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat.Theoretical Applied and Genetics,1989(77):689-700.
[64] Shewry P R,Halford N G and Tatham A S.The high molecular weight subunits of wheat, barley and rye:Genetics,molecular biology,chemistry and role in wheat gluten structure and functionality.1989,Pages 163-219 in:Oxford Surveys of Plant and Molecular Cell Biology,Vol 6.B.J.Miflin,ed.Oxford University Press:London.
[65] Miles M J,Carr H J,MacMaster T C,et al.Scanning tunneling microscopy of a wheat seed storage protein reveals details of an unusual supersecondary structure.Proceedings of the National Academy of Sciences of the USA.1991(88):68-71.
[66] Macritchie F.Physicochemical properties of wheat proteins in relation to functionality. Advances Food Nutrition Research.1992(36):1-87.
[67] Wrigley C W.Giant proteins with flour power.Nature,1996(381):738-739.
[68] Tatham A S,Shewry P R,Miflin B J.Wheat gluten elasticity–a similar molecular basis to elastin.FEBS Lett 1984(177):205-208.
[69]孙辉,王岳光,李宝云,等.小麦谷蛋白亚基及其基因多态性研究进展[J].麦类作物学报,2000(1) 47-53.
[70] Payne P I,Holt L M,Worland A J,et al.Structural and genetical studies on the high molecular weight subunits of wheat glutenin in part 3.Telocentric mapping of the subunit genes on the long arm of the homoeologous group 1 chromosomes.Theoretical and Applied Genetics, 1982(63:)129-138.
[71] He Z H,Pena R J,Rajaram S.High molecular weight glutenin subunit composition of Chinese bread wheat.Euphytica,1992(64):11-20.
[72]傅宾孝,赵友梅,秦礼谦.小麦高分子量麦谷蛋白亚基与面粉品质[J].郑州粮食学院学报,1989(1):8-11.
[73]赵友梅,王淑俭.高分子量麦谷蛋白亚基的SDS-PAGE图谱在小麦品质研究中的应用[J].作物学报,1990,16(3):208-218.
[74]赵和,卢少源,李宗智.普通小麦高分子量麦谷蛋白亚基遗传变异及其与其它性状的关系[J].河北农业大学学报,1993(1):8-12.
[75]程爱华,王乐凯,赵乃新.高分子量麦谷蛋白亚基评分系统的改进和应用[J].麦类作物学报,2002,22(1):19-22.
[76] Jauhar P P, Chibbar R N. Chromosome-mediated and direct gene transfers in wheat [J]. Genom e., 1999(42):570-583.
[77] Singh N K,Shepherd K W,Mcintosh R A . Linkage mapping of genes for resistance to leaf,stem,and stripe rusts and w-secalins on the short arm of rye chromosome 1R[J]. Theoretical Applied and Genetics,1990(80):609-616.
[78] Villareal R L,Mujeeb-Kazi A,Rajaram S,etal. Associated effects of chromosome 1B/1R translocation on agronomic traits in hexaploid wheat[J].Breed Sci.,1994(44):7-11.
[79] Villareal R L,Banuelos O,Mujeeb-Kazi A,etal. Agronomic performance of chromosome 1B and 1BL/1RS near isolines in the spring bread wheatSeriM[J]. Euphytica,1998(103):195-202.
[80]张正斌.小麦遗传学[M].北京:中国农业出版社, 2001: 39:158.
[81]周阳,何中虎,张改生,等.1BL/1RS品种在我国小麦育种中的应用[J].作物学报,2004,30(6):531-535.
[82] Pe?a R J, Amaya A, Rajaram S, Mujeep-Kazi A.Variation in quality of characteristics associated with some spring 1B/1R translocation wheats[J]. Journal of Cereal Science, 1990(12): 105-112.
[83]刘建军,何中虎,Pena R J,赵振东.1BL/1RS易位对小麦加工品质的影响[J].作物学报,2004,30(2):149-153.
[84]张立平,何中虎,陆美琴,等.用Glu-B3,Gli-B1和SEC-1b复合引物PCR检测普通小麦1BL/1RS易位系[J].中国农业科学, 2003, 36(12):1566-1570.
[85] Graybosch R A. Uneasy Unions: Quality effects of rye chromatin transfers to wheat[J].Journal of Cereal Science,2001(33):3-16.
[86] Huang W N,Hoseney R C. Isolocation and identification of a wheat flour compound causing sticky dough.[J].Cereal Chem,1999,76(2):276-281.
[87] Barbeau W E, Griffey C A, Uriyo M G, Harris C H, Johnson J M.Baking performance of 1BLP1RS soft red winter wheat[J].J Food Quality,2001,24(2):127-139。
[88]于亚雄,刘丽,胡银星,程耿,等.1BL/1RS易位系对小麦面筋品质的影响[J].西南农业学报,2004(6):685-689.
[89]刘丽,周阳,刘建军,何中虎,等.Gli-1和Glu-3等位变异及1BL/1RS易位与面包和面条品质关系的研究[J].中国农业科学,2004,37(9):1265-1273.
[90]陈东升,刘丽,董建力,何中虎,等.HMW-GS和LMW-GS组成及1BL/1RS易位系对春小麦品质性状的影响[J].作物学报,2005,31(4):414-419.
[91]董超华,徐如宏,张庆勤.小麦谷蛋白和醇溶蛋白研究进展[J].山地农业生物学报,2003,22(2):164-168.
[92] Laggudah ES,O’Brien L,Halloran GM.Influence of gliadin composition and high molecular weight subunits of glutenin on dough properties in an F3 population of a bread wheat cross[J].Journal of Cereal Science, 1988(7):33-42.
[93] Payne PI, Nightingale MA, Krattger AF,etal. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties[J].J.Sci. Food Agric.,1987(40):51-65.
[94] Lookhart L K,Kasard D D.High-molecular-weight glutenin subunit of the most commomly grown wheat cultivars in U.S.in 1984[J].Plant Breeding,1993(110):48-62.
[95] Lukow O M,Panye P I,Tacuk R.The HMW glutenin subunit composition of Canadian and association with bread-baking quality in progebies of six crosses of bread wheat[J].J.Sci.Food Agric.,1989(32):51-60.
[96]马啸,晏本菊,任正隆,等.四川省部分小麦新品系醇溶蛋白遗传多样性分析[J].四川农业大学学报, 2001, 19(4):384-388.
[97]任正隆.黑麦种质导入小麦及其在小麦育种中的利用方式[J].中国农业科学, 1991,24(3):18-25.
[98] Payne P I, Cordield K G, Blackman J A. Identification of a high-molecular subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree. Theoretical andApplied Genetics, 1992(55): 153-159.
[99]冯毅,闵东红,孙道杰,等.黄淮麦区部分推广小麦品种高分子量麦谷蛋白亚基组成分析[J].麦类作物学报,2006,26(2):68-71.
[100]王银萍,李勇超,魏燕燕,等.黄淮麦区小麦品种高分子量谷蛋白亚基组成及遗传多样性分析[J].麦类作物学报,2006,26(5):69-73.
[2]李鸿恩.我国小麦种质资源主要品质特性鉴定结果及评价[J].中国农业科学,1995,28(5):29-37.
[3]马传喜,徐风,程国旺.高分子量麦谷蛋白亚基作为面包小麦品种烘烤品质评价指标的研究[J].安徽农业大学学报,1995(2):103-107.
[4]刘东涛,冯国华,谢佩松,等.黄淮麦区小麦品种高分子量谷蛋白亚基组成分析[J].麦类作物学报2007(4):645-648.
[5] D.R马歇克,等著,朱厚础译.蛋白质纯化与鉴定实验指南[M].北京:科学技术出版社,1999.
[6]孙辉,李保云,王岳光,等.普通小麦谷蛋白亚基与烘烤品质的关系[J].中国农业大学学报, 2000(3):118-123.
[7]刘华,王宇生,张辉,等.小麦种质资源醇溶蛋白指纹图谱数据库的初步建立及应用[J].作物学报,1999,25(6):674-682.
[8]曹莉,王辉,李学军.黄淮冬麦区区试小麦品种(系)的品质性状分析及评价[J].麦类作物学报,2001,21(3):68-71.
[9]陆燕,马传喜.小麦品种麦谷蛋白亚基的品质效应研究进展[J].安徽农业大学学报:自然科学版,2000,(2):89-94.
[10]雷东锋,马建岗,赵文明.小麦种子贮藏蛋白质研究进展[J].西北植物学报,2001(3):67-69.
[11]李学军,王辉,李立群,等.高分子量麦谷蛋白亚基的品质效应研究进展[J].干旱地区农业研究,2003(3): 19-22.
[12]刘凤云,黄世全,张大乐,等.河南地区部分小麦新品种高分子量谷蛋白亚基组成分析[J].河南大学学报:自然科学版,2008(1):71-75.
[13]刘凤云,黄世全,张大乐,等.我国部分优质小麦醇溶蛋白遗传多样性分析[J].河南师范大学学报:自然科学版,2008(1):107-110.
[14] Zhang Xueyong,Yang Xueshen,Dong Yuchen.Genetic analysis of germplasm by acid polyacrylamide gel electrophoresis of gliadin[J].Scientia Agricultura Sinica,1995,28(4):25-32.
[15]李玉莲,黄承彦.小麦高分量麦谷蛋白亚基研究进展[J].山东农业科学,2004(5):52-54.
[16] Branlard G.Study of genetic determination of 20 gliadin bands.Theoretical Applied and Geneti-cs,1983(64):155-162.
[17] Metakovsky E V,Knezevich D,Javornik.Gliadin allele composition of Yugoslav winter wheat cultivars.Euphytica,1991(54):85-295.
[18]郎明林,卢少源,赵家发.适合我国小麦醇溶蛋白指纹图谱绘制的改良A-PAGE分子标记技术[J].北农业大学学报,1998(4):1-5.
[19]晏月明,茹岩岩,余建中,等.中国小麦品种醇溶蛋白Gli-1和Gli-2编码位点等位基因组成分析[J].农业生物技术学报,2000,8(1):23-27.
[20]晏月明,刘广田,Prodanovic,等.小麦醇溶蛋白基因的HPCE和A-PAGE染色体定位及其比较分析[J].农业生物技术学报,1998(2):67-71.
[21]魏育明,郑有良,刘登才等.四川小麦地方品种Gli-1、Gli-2和Glu-1位点的遗传多样性[J].植物学报,2000,42(5):496-501.
[22] Woychik J H,Boundy J A,Dimler R J.Starch gel electrophoresis of wheat gluten proteins with concentrated urea.Arch Biochem Biophys,1961(94):477-482.
[23] Gubareva N K,Gavrilyuk I P and Chernoburova A D.Determination of the identity and the cultivar purity of wheat grain according to electrophoretic patterns of the gliadin.Experiment-al methods and catalogue of cultivar electrophoregrams (in Russian).Vaskhnil (Leningrad).1998(67):1-39.
[24] Bushuk W,Zillman R R.Wheat cultivar identification by gliadin electrophoregrams:I. Apparatus,method and nomenclature.Canadian Journal of Plant Science,1978(58):505-515.
[25]颜启传,黄亚军,徐媛.试用ISTA推荐的种子醇溶蛋白电泳方法鉴定大麦和小麦品种[J].1992,18(l):65-67.
[26]阎旭东,卢少源,李宗智.适用于我国的小麦醇溶蛋白分析的A-PAGE方法[J].河北农业大学学报,1994,17(1):7-10.
[27]郎明林,卢少源,赵家发.适合我国小麦醇溶蛋白指纹图谱绘制的改良A-PAGE分子标记技术[J].河北农业大学学报,1998,21(4):1-5.
[28]王学路,钱曼茅,宋春华,等.ISTA醇溶蛋白电泳方法的改进和应用[J].作物遗传资源,1994(2):32-34.
[29]孙立新,张来群,孙海虹,等.小麦醇溶蛋白酸性电泳条件的探索[J].麦类作物学报.1999,25(1):126-129.
[30]邵锦震,丁毅.麦醇溶蛋白A-PAGE方法的优化和改进[J].武汉大学学报(自然科学版),2000(6):733-738.
[31]邵锦震,邱昌恩,丁毅.不同提取剂对麦醇溶蛋白提取效果的比较[J].武汉植物学研究,2003,21(3):262-266.
[32]晏月明,刘广田.小麦醇溶蛋白的遗传与品质改良[J],麦类作物学报,1998,18(l):1-5.
[33]晏月明,刘广田, S Prodanovic等.小麦醇溶蛋白和谷蛋白亚基高校毛细管分离研究[J].中国粮油学报, 1998, 13(4):1-5.
[34]余建中.小麦醇溶蛋白和高分子量谷蛋白亚基高效毛细管电泳研究.首都师范大学,硕士论文,2002.
[35] Devos K M, Bryan G J, Collins A j, et al. Application of two microsatellite sequences in wheat storage proteins as molecular markers[J].Theoretical Applied and Genetics, 1995(90):247-252.
[36] Okita T W,Cheesbrough V,Reeves C D.Evolution and heterogeneity of theα/β-type andγ-type gliadin DNA sequences.J Biol Chem,1985(260):8203-8213.
[37] Lee M. DNA markers and plant breeding programs[J].Adv agron,1995(55):265-344.
[38] Sobko T A, Poperelya F A, Ribalka A I, et al. Inheritance and mapping on chromosome 1A of genes coding for storage proteins in common wheat [J].Tsitol genet, 1986(20):372-376.
[39] Payne PI.Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality[J].Annu Rev Plant Physiol,1987(38):141-153.
[40] Shepherd K W.Genetics of wheat endosperm protein in retrospect and prospect.In Proceeding 7th Genetic Symposium.England Cambridge,1988(6):919-931.
[41] Redaelli R,et al.Two-dimensional mapping of gliadin using biotypes and null mutants of common wheat cultivar Saratovskaya 29.Heredita,1994(121):131-137.
[42] Day P R.The way ahead.Wheat breeding for quality improvement.Chemistry and Physics of Baking.1985(22):251-261.
[43] Mcintosh R A,Hart G E,Gale M D.Catalogue of gene symbols for wheat.Proc 9th Inter Wheat Genet Symp 1998(5):123-127.
[44] Sobko T A,Poperelya F A,Ribalka A I,et al.Inheritance and mapping on chromosome 1A of genes coding for storage proteins in common wheat(in Russian).Tsitol genet,1986(20):372-376.
[45] Metakovsky E V,Akhmadov M G and Sozinov A A.Genetic analysis of gliadin-encoding genes reveals gene clusters as well as single remote genes.Theoretical Applied and Genetics, 1986(73):278-285.
[46] Pogna,N E.Recombination mapping of Gli-5,a new gliadin coding locus on chromosomes 1A and 1B in common wheat.Theoretical Applied and Genetics,1993(87):113-121.
[47] Metakovsky E V,Novoselskaya A Yu,Kopus M M,et al.Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electrophoresis.Theoretica Applied andGenetics.1984(67):559-568.
[48] Metakovsky E V.Gliadin allele identification in common wheat II Catalogue of gliadin alleles in common wheat.Journal Genetics&Breeding,1991(45):325-344.
[49] Metakovsky E V,Brandland G.Genetic diversity of French wheat germplasm based on gliadin alleles.Theoretical Applied and Genetics,1998(96):209-218.
[50]贾继增,张正斌,Devos K,等.小麦21条染色体RFLP作图位点遗传多样性分析[J].中国农业科学,2001(1):13-21.
[51] Metakovsky E V,Annicchiarico P,Boggini G,et al.Relationship between gliadin alleles and dough strength in Italian bread wheat cultivars.Journal of Cereal Science,1997(25):229-236.
[52] Branlard G. Diversity of grain proteins and bread wheat quality I.correlation between gliadin bands and flour quality characteristics[J]. Cultivars J cereal Sci. ,1985(13):329-343.
[53] Payne P I,Kathyrn G C,Linda M H,et al.Correlations between the inheritance of certain high molecular weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat.Journal of the Science of Food and Agriculture,1981(32):51-60.
[54]巩普遍,赵会贤,郭蔼光.小偃6号HMW-GS表达动态研究[J].西北农林科技大学学报:自然科学版,2002(1):65-67.
[55]张学勇,杨欣明,董玉琛.醇溶蛋白电泳在小麦种质资源遗传分析中的应用[J].中国农业科学,1995,25(4):25-32.
[56] Shewry PR, Halford NG, Tatham AS. High molecular weight subunits of wheat glutenin[J]. Journal of Cereal Science, 1992(15):105-120.
[57]黄兴峰,马传喜,司红起,等.小麦品种高分子量谷蛋白亚基的组成分析[J].安徽农业大学学报,2003(4):60-66.
[58] Burnouf T,Bouriquet R.Glutenin subunits of genetically related European hexaploid wheat cultivars:their relation to breadmaking quality.Theoretical Applied and enetics,1980(58):107-111.
[59] Moonen,J H E,Scheepstra A and Graveland A.Use of the SDS-sedimentation test and SDS-polyacrylamide gel electrophoresis for screening breeder’s samples of wheat for bread-making quality.Euphytica,1982(31):677-690.
[60] Lawrence G J and Shepherd K W.Variation in glutenin protein subunits of wheat.Aust.J.Biol. Sci,1980(33):221-233.
[61] Payne P I,Lawrence G J.Catalogue of alleles for the complex gene loci,Glu-A1,Glu-B1 and Glu-D1 which code for high molecular weight subunits of glutenin in hexaploid wheat.Cereal Research Communication,1983(11):29-35.
[62] Ng P K W,Pogna N E,Mellini F et al.Glu-1 allele compositions of the wheat cultivars registered in Canada.J.Genet and Breed.1989(43):53-59.
[63] Anderson O D,Green F C.The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat.Theoretical Applied and Genetics,1989(77):689-700.
[64] Shewry P R,Halford N G and Tatham A S.The high molecular weight subunits of wheat, barley and rye:Genetics,molecular biology,chemistry and role in wheat gluten structure and functionality.1989,Pages 163-219 in:Oxford Surveys of Plant and Molecular Cell Biology,Vol 6.B.J.Miflin,ed.Oxford University Press:London.
[65] Miles M J,Carr H J,MacMaster T C,et al.Scanning tunneling microscopy of a wheat seed storage protein reveals details of an unusual supersecondary structure.Proceedings of the National Academy of Sciences of the USA.1991(88):68-71.
[66] Macritchie F.Physicochemical properties of wheat proteins in relation to functionality. Advances Food Nutrition Research.1992(36):1-87.
[67] Wrigley C W.Giant proteins with flour power.Nature,1996(381):738-739.
[68] Tatham A S,Shewry P R,Miflin B J.Wheat gluten elasticity–a similar molecular basis to elastin.FEBS Lett 1984(177):205-208.
[69]孙辉,王岳光,李宝云,等.小麦谷蛋白亚基及其基因多态性研究进展[J].麦类作物学报,2000(1) 47-53.
[70] Payne P I,Holt L M,Worland A J,et al.Structural and genetical studies on the high molecular weight subunits of wheat glutenin in part 3.Telocentric mapping of the subunit genes on the long arm of the homoeologous group 1 chromosomes.Theoretical and Applied Genetics, 1982(63:)129-138.
[71] He Z H,Pena R J,Rajaram S.High molecular weight glutenin subunit composition of Chinese bread wheat.Euphytica,1992(64):11-20.
[72]傅宾孝,赵友梅,秦礼谦.小麦高分子量麦谷蛋白亚基与面粉品质[J].郑州粮食学院学报,1989(1):8-11.
[73]赵友梅,王淑俭.高分子量麦谷蛋白亚基的SDS-PAGE图谱在小麦品质研究中的应用[J].作物学报,1990,16(3):208-218.
[74]赵和,卢少源,李宗智.普通小麦高分子量麦谷蛋白亚基遗传变异及其与其它性状的关系[J].河北农业大学学报,1993(1):8-12.
[75]程爱华,王乐凯,赵乃新.高分子量麦谷蛋白亚基评分系统的改进和应用[J].麦类作物学报,2002,22(1):19-22.
[76] Jauhar P P, Chibbar R N. Chromosome-mediated and direct gene transfers in wheat [J]. Genom e., 1999(42):570-583.
[77] Singh N K,Shepherd K W,Mcintosh R A . Linkage mapping of genes for resistance to leaf,stem,and stripe rusts and w-secalins on the short arm of rye chromosome 1R[J]. Theoretical Applied and Genetics,1990(80):609-616.
[78] Villareal R L,Mujeeb-Kazi A,Rajaram S,etal. Associated effects of chromosome 1B/1R translocation on agronomic traits in hexaploid wheat[J].Breed Sci.,1994(44):7-11.
[79] Villareal R L,Banuelos O,Mujeeb-Kazi A,etal. Agronomic performance of chromosome 1B and 1BL/1RS near isolines in the spring bread wheatSeriM[J]. Euphytica,1998(103):195-202.
[80]张正斌.小麦遗传学[M].北京:中国农业出版社, 2001: 39:158.
[81]周阳,何中虎,张改生,等.1BL/1RS品种在我国小麦育种中的应用[J].作物学报,2004,30(6):531-535.
[82] Pe?a R J, Amaya A, Rajaram S, Mujeep-Kazi A.Variation in quality of characteristics associated with some spring 1B/1R translocation wheats[J]. Journal of Cereal Science, 1990(12): 105-112.
[83]刘建军,何中虎,Pena R J,赵振东.1BL/1RS易位对小麦加工品质的影响[J].作物学报,2004,30(2):149-153.
[84]张立平,何中虎,陆美琴,等.用Glu-B3,Gli-B1和SEC-1b复合引物PCR检测普通小麦1BL/1RS易位系[J].中国农业科学, 2003, 36(12):1566-1570.
[85] Graybosch R A. Uneasy Unions: Quality effects of rye chromatin transfers to wheat[J].Journal of Cereal Science,2001(33):3-16.
[86] Huang W N,Hoseney R C. Isolocation and identification of a wheat flour compound causing sticky dough.[J].Cereal Chem,1999,76(2):276-281.
[87] Barbeau W E, Griffey C A, Uriyo M G, Harris C H, Johnson J M.Baking performance of 1BLP1RS soft red winter wheat[J].J Food Quality,2001,24(2):127-139。
[88]于亚雄,刘丽,胡银星,程耿,等.1BL/1RS易位系对小麦面筋品质的影响[J].西南农业学报,2004(6):685-689.
[89]刘丽,周阳,刘建军,何中虎,等.Gli-1和Glu-3等位变异及1BL/1RS易位与面包和面条品质关系的研究[J].中国农业科学,2004,37(9):1265-1273.
[90]陈东升,刘丽,董建力,何中虎,等.HMW-GS和LMW-GS组成及1BL/1RS易位系对春小麦品质性状的影响[J].作物学报,2005,31(4):414-419.
[91]董超华,徐如宏,张庆勤.小麦谷蛋白和醇溶蛋白研究进展[J].山地农业生物学报,2003,22(2):164-168.
[92] Laggudah ES,O’Brien L,Halloran GM.Influence of gliadin composition and high molecular weight subunits of glutenin on dough properties in an F3 population of a bread wheat cross[J].Journal of Cereal Science, 1988(7):33-42.
[93] Payne PI, Nightingale MA, Krattger AF,etal. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties[J].J.Sci. Food Agric.,1987(40):51-65.
[94] Lookhart L K,Kasard D D.High-molecular-weight glutenin subunit of the most commomly grown wheat cultivars in U.S.in 1984[J].Plant Breeding,1993(110):48-62.
[95] Lukow O M,Panye P I,Tacuk R.The HMW glutenin subunit composition of Canadian and association with bread-baking quality in progebies of six crosses of bread wheat[J].J.Sci.Food Agric.,1989(32):51-60.
[96]马啸,晏本菊,任正隆,等.四川省部分小麦新品系醇溶蛋白遗传多样性分析[J].四川农业大学学报, 2001, 19(4):384-388.
[97]任正隆.黑麦种质导入小麦及其在小麦育种中的利用方式[J].中国农业科学, 1991,24(3):18-25.
[98] Payne P I, Cordield K G, Blackman J A. Identification of a high-molecular subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree. Theoretical andApplied Genetics, 1992(55): 153-159.
[99]冯毅,闵东红,孙道杰,等.黄淮麦区部分推广小麦品种高分子量麦谷蛋白亚基组成分析[J].麦类作物学报,2006,26(2):68-71.
[100]王银萍,李勇超,魏燕燕,等.黄淮麦区小麦品种高分子量谷蛋白亚基组成及遗传多样性分析[J].麦类作物学报,2006,26(5):69-73.