硝基咪唑类药物的电化学行为及其与血清白蛋白的相互作用研究
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摘要
第一章:硝基咪唑类药物现已广泛应用于兽药和人医临床,但是由于它们具有潜在的致癌、致突变性,所以其在人体和动物体内的残留对人类健康构成了严重威胁。本章详细介绍了硝基咪唑类药物的性质及其分析方法研究进展。药物与血清白蛋白的相互作用研究是现阶段科学家研究的热点课题,本章综述了药物与血清蛋白质相互作用的研究状况。近年来,电化学分析在方法、技术和应用方面得到了长足的发展,本章对近年来的电化学研究方法,及其优越性做了详细介绍。化学修饰电极与普通电极相比可以增强被测物在电极表面的电化学信号,特别是碳纳米管修饰电极越来越受电化学工作者们的重视,本章详细介绍了化学修饰电极和碳纳米管修饰电极的研究概况。
第二章:本章主要介绍了甲硝唑在银盘电极上的电化学行为,并对甲硝唑片剂进行了检测,实验证明,该方法可以为甲硝唑药品检验提供一种新的检测手段。并用循环伏安法研究了甲硝唑还原峰的性质,发现电极反应是完全不可逆的,并存在一定的吸附性。
第三章:碳纳米管修饰电极是现阶段科学家们的研究热点,碳纳米管有其独特的性能,如比表面积大、化学性质稳定、机械性能好、灵敏的电子传递性能,正因为碳纳米管有如此特性,把它用于电极材料时可以加快电极表面的电子传递速度,从而使电极表面的电化学反应充分进行。在本章中,采用多壁碳纳米管修饰的玻碳电极为工作电极来研究奥硝唑在其表面的电化学行为,奥硝唑在该电极上有一灵敏的还原峰,而在裸玻碳电极上则几乎没有峰。
第四章:研究药物与血清白蛋白的作用有助于阐明药物在体内的存储和转运过程,也能为药物的设计与开发及药理研究提供有价值的信息。本章采用电化学方法研究了甲硝唑与牛血清白蛋白的相互作用,实验结果表明,甲硝唑在-0.57V(vs.SCE)处出现一灵敏的还原峰,而牛血清白蛋白(BSA)在扫描范围为-0.3～-0.8V处没有峰。当牛血清白蛋白加入到甲硝唑溶液中时,甲硝唑的还原峰电流下降,该还原峰电流的下降值与BSA加入量呈线性关系,并对二者的相互作用机理进行了探讨,二者主要通过疏水键相结合。
Chapter 1:5-Nitroimidazoles had been widely used in animal drugs and human clinical medicine,the carcinogensis and mutability of nitroimidazoles damage human health because of its residual drugs,physicochemical property and analytical method research progressivity of nitroimidazolesh have been introduced in this chapter.Scientists are interesting in study of interactivity between drug and serum albumin in present,in this chapter, interactivity between drug and serum albumin has been reviewed.Superiority of electrochemical methodology is developed about method,technology and application widely.The electrochemical methods and its application have been introduced in this chapter.Compared with normal electrode,chemical modified electrode can strengthen electrochemical signal of detected materials,carbon nanotube modified electrodes are attention specially by electrochemical workers.In this chapter,study of chemical modified electrode and carbon nanotube modified electrodes has been introduced.
Chapter 2:The electrochemical behavior and determination of metronidazole on the silver disk electrode have been studied in this chapter, metronidazole tablets have been detected,the results showed that this method can be used for the determination and pharmacokinetics study of metronidazole.The current property of reduction peak of metronidazole was investigated by the cyclic voltammetry.The characteristic irreversible adsorption of the peak was proved.
Chapter 3:Electrodes based on carbon nanotubes are an attractive research area now.Carbon nanotubes display remarkable nanostructures in that they combine large surface area、excellent electrical conductivity、good chemical stability and significant mechanical strength.The subtle electronic properties suggest that carbon nanotubes have the ability to promote electron-transfer reactions when used as an electrode material in electrochemical reactions,which represents a new area of application for carbon nanotubes.In this study,the MWNTs were dropped onto the surface of a glassy carbon(GC) electrode to form an MWNT-modified one.The electrochemical behavior of ornidazole at such a modified electrode was investigated in detail,a reduction peak was obtained in ornidazole solution at the MWNT-modified electrode,compared with a bare electrode,the peak current had obviously increased.
Chapter 4:Study on the interaction drugs and serum albumin help clarify drugs save and operation process,at the same time,it can provide valuable information regarding drug design、development and pharmacology research.In this chapter,the electrochemical methods are used for study on the interaction between metronidazole and bovine serum albumin.The results showed that a sensitive reduction peak of metronidazole has been obtained, the peak potential was-0.57V(vs.SCE).But bovine serum albumin has no reduction peak in the scanning area of-0.3～-0.8V.When bovine serum albumin was added in metronidazole solution,the reduction peak of metronidazole will fall down,the peak current declining value was directly proportional to the bovine serum albumin concentration.In addition,the interaction mechanism between metronidazole and BSA has been studied,the results demonstrate that combination of them mainly by hydrophobicity.
第二章:本章主要介绍了甲硝唑在银盘电极上的电化学行为,并对甲硝唑片剂进行了检测,实验证明,该方法可以为甲硝唑药品检验提供一种新的检测手段。并用循环伏安法研究了甲硝唑还原峰的性质,发现电极反应是完全不可逆的,并存在一定的吸附性。
第三章:碳纳米管修饰电极是现阶段科学家们的研究热点,碳纳米管有其独特的性能,如比表面积大、化学性质稳定、机械性能好、灵敏的电子传递性能,正因为碳纳米管有如此特性,把它用于电极材料时可以加快电极表面的电子传递速度,从而使电极表面的电化学反应充分进行。在本章中,采用多壁碳纳米管修饰的玻碳电极为工作电极来研究奥硝唑在其表面的电化学行为,奥硝唑在该电极上有一灵敏的还原峰,而在裸玻碳电极上则几乎没有峰。
第四章:研究药物与血清白蛋白的作用有助于阐明药物在体内的存储和转运过程,也能为药物的设计与开发及药理研究提供有价值的信息。本章采用电化学方法研究了甲硝唑与牛血清白蛋白的相互作用,实验结果表明,甲硝唑在-0.57V(vs.SCE)处出现一灵敏的还原峰,而牛血清白蛋白(BSA)在扫描范围为-0.3～-0.8V处没有峰。当牛血清白蛋白加入到甲硝唑溶液中时,甲硝唑的还原峰电流下降,该还原峰电流的下降值与BSA加入量呈线性关系,并对二者的相互作用机理进行了探讨,二者主要通过疏水键相结合。
Chapter 1:5-Nitroimidazoles had been widely used in animal drugs and human clinical medicine,the carcinogensis and mutability of nitroimidazoles damage human health because of its residual drugs,physicochemical property and analytical method research progressivity of nitroimidazolesh have been introduced in this chapter.Scientists are interesting in study of interactivity between drug and serum albumin in present,in this chapter, interactivity between drug and serum albumin has been reviewed.Superiority of electrochemical methodology is developed about method,technology and application widely.The electrochemical methods and its application have been introduced in this chapter.Compared with normal electrode,chemical modified electrode can strengthen electrochemical signal of detected materials,carbon nanotube modified electrodes are attention specially by electrochemical workers.In this chapter,study of chemical modified electrode and carbon nanotube modified electrodes has been introduced.
Chapter 2:The electrochemical behavior and determination of metronidazole on the silver disk electrode have been studied in this chapter, metronidazole tablets have been detected,the results showed that this method can be used for the determination and pharmacokinetics study of metronidazole.The current property of reduction peak of metronidazole was investigated by the cyclic voltammetry.The characteristic irreversible adsorption of the peak was proved.
Chapter 3:Electrodes based on carbon nanotubes are an attractive research area now.Carbon nanotubes display remarkable nanostructures in that they combine large surface area、excellent electrical conductivity、good chemical stability and significant mechanical strength.The subtle electronic properties suggest that carbon nanotubes have the ability to promote electron-transfer reactions when used as an electrode material in electrochemical reactions,which represents a new area of application for carbon nanotubes.In this study,the MWNTs were dropped onto the surface of a glassy carbon(GC) electrode to form an MWNT-modified one.The electrochemical behavior of ornidazole at such a modified electrode was investigated in detail,a reduction peak was obtained in ornidazole solution at the MWNT-modified electrode,compared with a bare electrode,the peak current had obviously increased.
Chapter 4:Study on the interaction drugs and serum albumin help clarify drugs save and operation process,at the same time,it can provide valuable information regarding drug design、development and pharmacology research.In this chapter,the electrochemical methods are used for study on the interaction between metronidazole and bovine serum albumin.The results showed that a sensitive reduction peak of metronidazole has been obtained, the peak potential was-0.57V(vs.SCE).But bovine serum albumin has no reduction peak in the scanning area of-0.3～-0.8V.When bovine serum albumin was added in metronidazole solution,the reduction peak of metronidazole will fall down,the peak current declining value was directly proportional to the bovine serum albumin concentration.In addition,the interaction mechanism between metronidazole and BSA has been studied,the results demonstrate that combination of them mainly by hydrophobicity.
引文
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