榆黄蘑的化学成分研究
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摘要
榆黄蘑又名金顶蘑或称金顶侧耳(pleurotus citrinopileatus sing.),属于担子菌亚门,层菌纲,伞菌目,侧耳科,侧耳属,是一种珍贵的经济菌物。人们常食用,味美,香甜,具有一定的医疗保健作用。近年来研究表明榆黄蘑主要含有甾醇类和脂肪酸等成分。但目前对榆黄蘑有效成分的研究并不全面,药理学的研究也刚刚起步。作者通过硅胶柱层析,凝胶柱层析等分离手段从榆黄蘑中分离出8个单体化合物,通过理化性质和现代波谱学方法和技术(ESI-MS、~1HNMR、~(13)CNMR等)分析,确定他们的结构分别为:甘露醇,麦角甾醇,3β,5α,6β-三羟麦角甾醇,腺嘌呤核苷,尿嘧啶,烟酰胺,肌苷,鸟苷。
利用HPLC—MS/MS对榆黄蘑中甘露醇进行定量。用MS/MS作为HPLC的检测器采用多反应检测(MRM)扫描方式,建立了HPLC-MS/MS测定榆黄蘑中甘露醇含量的方法。该方法的线性范围为0.208-1.04mg·mL~(-1)。通过样品的加标试验,回收率为97.12%,精密度RSD(n=5)为0.94%。实际样品分析表明,榆黄蘑中甘露醇的含量为38.9mg/g。本方法选择性高、灵敏、快速。也适用于其他复杂体系中甘露醇含量的快速测定。
Pleurotus citrinopileatus is belong to Basidiomycotina Hymenomycetes class Agaricales Pleurotus is one kind of precious economical fungus,People often edible, the taste is beautiful,fragrant,has certain healthcare function.This year studies indicated that Pleurotus citrinopileatus mainly includes fatty acid ingredients and so on sterol class.But present is not comprehensive to the Pleurotus citrinopileatus effective component's research.The Pharmacology research also just started.The author through the silica gel column chromatographic analysis,separation methods and so on gelatin column chromatographic analysis separates the monomer compound from Pleurotus citrinopileatus,Through physical and chemical properties,spectrum of modern methods and techniques(ESI-MS,1HNMR,13CNMR),Determined that their structure respectively is mannitol,ergosterol,ergotsa-7,22-dien-3β,5α,6β-triol,adenosine,uracil, nicotinamide,inosine,guanosine.
The mannitol carries on the quota using HPLC-MS/MS to Pleurotus citrinopileatus.Uses the multi-response examination with MS/MS as the HPLC detector (MRM) to scan the way,Has established in HPLC-MS/MS determination Pleurotus citrinopileatus the mannitol content method.This method's linearity range is 0.208-1.04mgmL-1.Through the sample Canadian sign experiment,the returns-ratio is 97.12%,accuracy RSD(n=5) is 0.94%.The actual sample analysis indicated that in Pleurotus citrinopileatus the mannitol content is 38.9mg/g.This method selectivity high, is keen,is fast.It also suitable in other complex systems the mannitol content fast determination.
利用HPLC—MS/MS对榆黄蘑中甘露醇进行定量。用MS/MS作为HPLC的检测器采用多反应检测(MRM)扫描方式,建立了HPLC-MS/MS测定榆黄蘑中甘露醇含量的方法。该方法的线性范围为0.208-1.04mg·mL~(-1)。通过样品的加标试验,回收率为97.12%,精密度RSD(n=5)为0.94%。实际样品分析表明,榆黄蘑中甘露醇的含量为38.9mg/g。本方法选择性高、灵敏、快速。也适用于其他复杂体系中甘露醇含量的快速测定。
Pleurotus citrinopileatus is belong to Basidiomycotina Hymenomycetes class Agaricales Pleurotus is one kind of precious economical fungus,People often edible, the taste is beautiful,fragrant,has certain healthcare function.This year studies indicated that Pleurotus citrinopileatus mainly includes fatty acid ingredients and so on sterol class.But present is not comprehensive to the Pleurotus citrinopileatus effective component's research.The Pharmacology research also just started.The author through the silica gel column chromatographic analysis,separation methods and so on gelatin column chromatographic analysis separates the monomer compound from Pleurotus citrinopileatus,Through physical and chemical properties,spectrum of modern methods and techniques(ESI-MS,1HNMR,13CNMR),Determined that their structure respectively is mannitol,ergosterol,ergotsa-7,22-dien-3β,5α,6β-triol,adenosine,uracil, nicotinamide,inosine,guanosine.
The mannitol carries on the quota using HPLC-MS/MS to Pleurotus citrinopileatus.Uses the multi-response examination with MS/MS as the HPLC detector (MRM) to scan the way,Has established in HPLC-MS/MS determination Pleurotus citrinopileatus the mannitol content method.This method's linearity range is 0.208-1.04mgmL-1.Through the sample Canadian sign experiment,the returns-ratio is 97.12%,accuracy RSD(n=5) is 0.94%.The actual sample analysis indicated that in Pleurotus citrinopileatus the mannitol content is 38.9mg/g.This method selectivity high, is keen,is fast.It also suitable in other complex systems the mannitol content fast determination.
引文
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[3]刘晓峰.李玉.孙晓波.等.榆黄蘑(Pleurotus citrinopileatus s)化学成分和药用活性的研究[J].吉林农业大学学报.1998.20(增刊):181.
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[7]Cai Z,Lee FSC,Wang XR,Yu WJ.A capsule review of recent studies on the application of mass sapectrometry in the analysis of Chinese medicinal herbs.J.Mass Spectrom.2002,37:1031-1024.
[8]He XG,On-line identification of phytochemical constituents in botanical extracts by combined high-performance liquid chromatographicdiode array detection-mass spectrometric techniques.J.Chromatogr.A 2000,880:203-232.
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[10]Sawabe Akiyoshi,Morim Masanori,Ouehi Seiji.Struetund analysesof glycolipids from edible n'lushl'Dom by fas t atom bombardment mass spectrometry[J].Stud Plant Sci,1999.6:346 · 359(Eng).
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[15]刘晓峰,李玉,孙晓波,徐惠波 侧耳属真菌化学成分及药用活性研究进展.吉林农业大学学报 1998,20(3):92-98.
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[19]Tomas Rezunka,Unusual and very long-chain fatty acids produced by Basidiomycetes journal of Chromatography.1987,(409):390-395.
[20]Marc Stadler er,al.Fatty Acids and other Compounds with Nematieidal Activity from Cultures of Busidiomycees,Planta Med.1994,60(2):128-132.
[21]曹瑞敏等.中国产“黄蘑”的化学成分的研究,静冈大学农学部研究报告,Bull.Fac Agric.Shizuoka Univ,1995.[45]:25-30.
[22]Bend G.Abraham and Rolf G.berger,Higher Fungi for Generating Aromn Components through Novel Biotechnologies,J.Agric.Food Chem.,1994,42:2344-2348.
[23]汪麟等.20种食用菌的氨基酸含量分析.食品科学,1985,(4).12-18.
[24]Manu-Tawish W and Matria A M.Chemical composition of Pleurous ostraius mycehal biomass.Food Microbiology.1987.(4).303-310.
[25]Litchchfield J H and Overbee R C.Submerged culture rowth Morchella species in food processing waste substances.Proceedings of the 1st inter.Congress on Food Sci and Technol.London.1965,2:511-520.
[26]吉田博等.担子菌菌丝体中的化学成分.日菌报.1996.(37)51-56.
[27]By Gubye Pfeffe.Systematic Distribution of Ribitol in the Fungi.New phytol,1990.(85):163-168.
[28]Hura Mitsunpobu 6-Deoxyilludin M.A New Antitunmor Antibiotic;Farmentation,Isolation and Structutal Identificution.J.Antilbiot.1987,40(11):1643-1646.
[29]Nakano;Hirofumi.Jpn.Kokai Tokkyo Koho JB62,234,040.
[30]Goldstem J L and Brown M S.Regulution of the mevealonste pathway.Nature.1990.343:425-430.
[31]Endo A et al.MI-236A.ML-236B.and ML-236C,new inhibitors of cholesterogenesis produced by Penicillium ritrinum.J.Antibiot,1976,29:1346-1348.
[32]Alberts A W et al.Mevinolin.a highly potent competiove inhibior of hydroxymethylgluroryl-ccenzyme.Proc.Narl.Acad.Sci:USA,1980,77:3975-3981.
[33]Nina Gunde-Cinerma et al.Pleurotus fungi produce mevinolin,an inhibitor of HMG CoA reductuse.FEMS Microbiology Letters.1993.113:332-338.
[34]Ninu Gunde-Cinermu et al.Screening Fungi for the Production of an Inhibitor of HMG CoA Reductase;Producion of Mevinolin by the Fungi of the Genus Pleurous.FEMS Microbiology Letters 1993.111:203-206.
[35]Yuko YoshioKa et al.Studies on Antitumor Activity of Some Fraction from Basidioniyces.Chem.Pharm.Bull.1972.20(6):1175-1180.
[36]Cuo Zhang et al.Antiunor Protein-containing Polysaccharides from a Chinese Mushroom Fengueiga or huabitake,Plearous sajor-caju(Ft.) Sings.Biosci Biotech.Biochem.1993.57(6):901-906.
[37]Yamurnoto Jpp.Tokkyo Koho 801597.
[38]江苏新医学院.中药大辞典[M].上海:上海科学技术出版社。1986:24-38
[39]王沛,宋启印.金顶蘑对动物机体免疫系统的影响[J].中国食用菌,1993,12(2):25-26.
[40]张丽萍,孙非,张翼伸.化学修饰对金顶侧耳多糖抗病毒(CB5)活性的影响[J].生物化学杂志,1994.10(2):19-21.
[41]刘小雷,张兴岐.香菇、榆黄蘑抗衰老研究[J].内蒙古医学院学报,1996,18(1):23-26.
[42]金宗濂,周宗俊.榆黄蘑发酵液的抗衰老研究[J].北京联合大学学报(自然版), 1991,5(2):8-12.
[43]李长田,李玉.金顶侧耳清除OH的能力研究[J].吉林农业大学学报,1998.20(增刊):109.
[44]王慧杰.食用菌的药用保健价值[J].食用菌.2001(3):41-42.
[45]Tam S C et al.Hypotensive and Renal Effects of an Exracts of the Edible Mushroom Pleurous sajor-caja.Life science.1986.38,1155-1161.
[46]Bobek et al.Choleserol Lowering Effect of the Mushroom Pleurots ostreaus in Herediary Hypercholesterolemicrate.Ann.Nutt.Metab.1991.35:191-195.
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