聚羟基脂肪酸酯纳米颗粒作为药物缓释载体的应用
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摘要
在本论文中,PHB、PHBHHx和PLA分别被制作成平均粒径为160nm、250nm和150nm的微小颗粒。一种脂溶性荧光染料罗丹明B(RBITC)被包裹到纳米颗粒中,作为检测胞吞效果及缓释过程的标记。实验中的三种材料所制备的纳米颗粒均能高效的包裹RBITC,装载效率高达75%。装载有RBITC荧光染料的纳米颗粒被原代巨噬细胞胞吞后,检测结果显示,PHB和PHBHHx纳米颗粒中的RBTIC在胞内的缓释可以持续至少20天,而PLA纳米颗粒中的RBITC在第15天就基本已经释放出来,不被材料包裹的RBITC溶液在巨噬细胞中不到7天就已经释放完全。缓释的速度不受载体材料和颗粒大小的差异影响。本研究首次证明PHB和PHBHHx能有效的作为药物载体应用于药物缓释。
In this study, Polyhydroxybutyrate (PHB), copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), and polylactic acid (PLA) were prepared into nanoparticles with average sizes of 160, 250 and 150 nm, respectively. A lipid soluble colorant rhodamine B isothiocyanate (RBITC) was employed to study drug release behaviors from these nanoparticles. A high RBITC drug loading efficiency of over 75% was achieved with all PHA nanoparticles prepared. Macrophage endocytosis led to an intracellular RBITC drug sustained release over a period of at least 20 days for PHB and PHBHHx nanoparticles, while PLA nanoparticles and free drug lasted only 15 days and a week, respectively. Polymer properties and particle sizes showed little effect on drug release behavior. This study showed for the first time that PHB and PHBHHx can be used effectively to achieve intracellular drug control releases.
In this study, Polyhydroxybutyrate (PHB), copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), and polylactic acid (PLA) were prepared into nanoparticles with average sizes of 160, 250 and 150 nm, respectively. A lipid soluble colorant rhodamine B isothiocyanate (RBITC) was employed to study drug release behaviors from these nanoparticles. A high RBITC drug loading efficiency of over 75% was achieved with all PHA nanoparticles prepared. Macrophage endocytosis led to an intracellular RBITC drug sustained release over a period of at least 20 days for PHB and PHBHHx nanoparticles, while PLA nanoparticles and free drug lasted only 15 days and a week, respectively. Polymer properties and particle sizes showed little effect on drug release behavior. This study showed for the first time that PHB and PHBHHx can be used effectively to achieve intracellular drug control releases.
引文
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