胃癌组织中胰岛素样生长因子-Ⅰ的表达与淋巴管密度的关系及其意义
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摘要
目的:研究胃癌组织中胰岛素样生长因子-Ⅰ表达与胃癌内淋巴管的关系及其意义;
方法:运用R-T PCR的方法检测38例胃癌组织及15例正常胃组织中胰岛素样生长因子-Ⅰ的表达情况,同时运用免疫组化检测其中的淋巴管及其密度;
结果:在正常胃黏膜中IGF-Ⅰ/GAPDH基因比值为0.138±0.012;而在胃癌组织中的表达比正常胃黏膜显著增高,IGF-Ⅰ/GAPDH基因比值为0.362±0.036;在高中分化的腺癌中IGF-Ⅰ/GADPH比值为0.351±0.026,低未分化癌中其值为0.425±0.022;在有淋巴转移的胃癌中这个比值为0.418±0.018,而在无淋巴转移的胃癌中为0.327±0.021;在Ⅰ-Ⅱ期的胃癌中为0.310±0.016,而在Ⅲ-Ⅳ期中为0.448±0.028。在不同性别的病例中,男女病例的比值分别为0.388±0.014及0.369±0.016;在不同年龄组中得到的比值分别为< 60岁0.405±0.015及≥60岁0.411±0.017。经统计学分析IGF-Ⅰ表达与胃癌临床分期(r=0.588, P<0.05),淋巴转移(r=0.693,P<0.05)相关,与分化程度呈负相关(r=-0.462, P<0.05);但与患者性别、年龄无关,均无统计学意义,P〉0.05。胃癌组织淋巴管密度平均9.52±0.54个,正常组织淋巴管密度平均4.29±0.66个;在高中分化的腺癌中LVD为7.52±0.21,低未分化癌中其值为10.81±0.33;在有淋巴转移的胃癌中为12.04±0.52,而在无淋巴转移的胃癌中为8.87±0.60;在Ⅰ-Ⅱ期的胃癌中为6.97±0.51,而在Ⅲ-Ⅳ期中为11.36±0.26。在不同性别的病例中,男女病例的比值分别为9.38±0.36及8.87±0.48;在不同年龄组中得到的比值分别为< 60岁10.56±0.45及≥60岁9.35±0.38;淋巴管密度与淋巴结转移(r=0.525, P<0.05),临床分期(r=0.683, P<0.05)表达相关,与分化程度负相关(r=-0.556, P<0.05),与IGF-Ⅰ的表达成显著正相关(r=0.732,P<0.001);与正常胃组织有显著差异,P<0.001;与性别、年龄无关,不具有统计学意义,P〉0.05。
结论:胃癌组织IGF-Ⅰ表达及淋巴管密度与胃癌分化程相关,与胃癌淋巴转移相关,与性别、年龄无关;IGF-Ⅰ与肿瘤淋巴管密度正相关;IGF-Ⅰ可能增加LVD加速肿瘤的淋巴转移。
Objective:To explore the relationship between the expression of IGF-Ⅰand LVD in gastric carcinoma .
Methods:S - P immunohistochemical method and R-T PCR was applied in detecting carcinoma samples of 38 cases.
Results:The mRNA levels of IGF-Ⅰwere increased in gastric cancer tissues compared with normal tissues from healthy controls ,P<0.001. The ratios of IGF-1/GADPH were 0.362±0.036 in gastric cancer tissues,but the ratios were 0.138±0.012 in the tumor free tissues. There was a significant difference of the levels of IGF-Ⅰbetween the group of TNMⅠ+Ⅱ(0.310±0.016)andⅢ+Ⅳ(0.448±0.028) P<0.05);The mRNA levels of IGF-Ⅰin metastatic lymph nodes were higher ( 0.418±0.018 ) than in without metastatic lymph nodes(0.327±0.021).The mRNA levels of IGF-Ⅰin low differentiation were higher (0.425±0.022)than middle-high differentiation(0.351±0.026).The expression of IGF-Ⅰwas positivily correlated with clinical stage(r=0.588, P<0.05),and lympha node metastasis(r=0.693, P<0.05),but was negatively correlated with pathologic differentiation(r=-0.462, P<0.05).
The ratios of LVD were 9.52±0.54 in gastric cancer tissues,but the ratios were 4.29±0.66 in the tumor free tissues. There was a significant difference of the levels of IGF-Ⅰbetween the group of TNMⅠ+Ⅱ(6.97±0.51)andⅢ+Ⅳ(11.36±0.26) P<0.05);The LVD in metastatic lymph nodes were higher (12.04±0.52)than in without metastatic lymph nodes(8.87±0.60).The LVD in low differentiation were higher (10.81±0.33)than middle-high differentiation(7.52±0.21).The expression of LVD was positivily correlated with clinical stage(r=0.683, P<0.05),lympha node metastasis(r=0.525, P<0.05,and IGF-Ⅰ(r=0.732,P<0.001),but was negatively correlated with pathologic differentiation(r=-0.556, P<0.05).The expression of IGF-Ⅰwas positivily correlated with LVD(r=0.732, P<0.01)
Conclusion:in gastric cancer, the expression rate of IGF-Ⅰand LVD was high. IGF-Ⅰexpression was positively correrated with LVD,and lympha node metastasis.It is presumed that IGF-Ⅰcan promote the metastasis of lyphma node in tumor by inducing LVD expression and the detection of IGF-Ⅰin gastic cancer tissues will be significance for prediction of progonsis
方法:运用R-T PCR的方法检测38例胃癌组织及15例正常胃组织中胰岛素样生长因子-Ⅰ的表达情况,同时运用免疫组化检测其中的淋巴管及其密度;
结果:在正常胃黏膜中IGF-Ⅰ/GAPDH基因比值为0.138±0.012;而在胃癌组织中的表达比正常胃黏膜显著增高,IGF-Ⅰ/GAPDH基因比值为0.362±0.036;在高中分化的腺癌中IGF-Ⅰ/GADPH比值为0.351±0.026,低未分化癌中其值为0.425±0.022;在有淋巴转移的胃癌中这个比值为0.418±0.018,而在无淋巴转移的胃癌中为0.327±0.021;在Ⅰ-Ⅱ期的胃癌中为0.310±0.016,而在Ⅲ-Ⅳ期中为0.448±0.028。在不同性别的病例中,男女病例的比值分别为0.388±0.014及0.369±0.016;在不同年龄组中得到的比值分别为< 60岁0.405±0.015及≥60岁0.411±0.017。经统计学分析IGF-Ⅰ表达与胃癌临床分期(r=0.588, P<0.05),淋巴转移(r=0.693,P<0.05)相关,与分化程度呈负相关(r=-0.462, P<0.05);但与患者性别、年龄无关,均无统计学意义,P〉0.05。胃癌组织淋巴管密度平均9.52±0.54个,正常组织淋巴管密度平均4.29±0.66个;在高中分化的腺癌中LVD为7.52±0.21,低未分化癌中其值为10.81±0.33;在有淋巴转移的胃癌中为12.04±0.52,而在无淋巴转移的胃癌中为8.87±0.60;在Ⅰ-Ⅱ期的胃癌中为6.97±0.51,而在Ⅲ-Ⅳ期中为11.36±0.26。在不同性别的病例中,男女病例的比值分别为9.38±0.36及8.87±0.48;在不同年龄组中得到的比值分别为< 60岁10.56±0.45及≥60岁9.35±0.38;淋巴管密度与淋巴结转移(r=0.525, P<0.05),临床分期(r=0.683, P<0.05)表达相关,与分化程度负相关(r=-0.556, P<0.05),与IGF-Ⅰ的表达成显著正相关(r=0.732,P<0.001);与正常胃组织有显著差异,P<0.001;与性别、年龄无关,不具有统计学意义,P〉0.05。
结论:胃癌组织IGF-Ⅰ表达及淋巴管密度与胃癌分化程相关,与胃癌淋巴转移相关,与性别、年龄无关;IGF-Ⅰ与肿瘤淋巴管密度正相关;IGF-Ⅰ可能增加LVD加速肿瘤的淋巴转移。
Objective:To explore the relationship between the expression of IGF-Ⅰand LVD in gastric carcinoma .
Methods:S - P immunohistochemical method and R-T PCR was applied in detecting carcinoma samples of 38 cases.
Results:The mRNA levels of IGF-Ⅰwere increased in gastric cancer tissues compared with normal tissues from healthy controls ,P<0.001. The ratios of IGF-1/GADPH were 0.362±0.036 in gastric cancer tissues,but the ratios were 0.138±0.012 in the tumor free tissues. There was a significant difference of the levels of IGF-Ⅰbetween the group of TNMⅠ+Ⅱ(0.310±0.016)andⅢ+Ⅳ(0.448±0.028) P<0.05);The mRNA levels of IGF-Ⅰin metastatic lymph nodes were higher ( 0.418±0.018 ) than in without metastatic lymph nodes(0.327±0.021).The mRNA levels of IGF-Ⅰin low differentiation were higher (0.425±0.022)than middle-high differentiation(0.351±0.026).The expression of IGF-Ⅰwas positivily correlated with clinical stage(r=0.588, P<0.05),and lympha node metastasis(r=0.693, P<0.05),but was negatively correlated with pathologic differentiation(r=-0.462, P<0.05).
The ratios of LVD were 9.52±0.54 in gastric cancer tissues,but the ratios were 4.29±0.66 in the tumor free tissues. There was a significant difference of the levels of IGF-Ⅰbetween the group of TNMⅠ+Ⅱ(6.97±0.51)andⅢ+Ⅳ(11.36±0.26) P<0.05);The LVD in metastatic lymph nodes were higher (12.04±0.52)than in without metastatic lymph nodes(8.87±0.60).The LVD in low differentiation were higher (10.81±0.33)than middle-high differentiation(7.52±0.21).The expression of LVD was positivily correlated with clinical stage(r=0.683, P<0.05),lympha node metastasis(r=0.525, P<0.05,and IGF-Ⅰ(r=0.732,P<0.001),but was negatively correlated with pathologic differentiation(r=-0.556, P<0.05).The expression of IGF-Ⅰwas positivily correlated with LVD(r=0.732, P<0.01)
Conclusion:in gastric cancer, the expression rate of IGF-Ⅰand LVD was high. IGF-Ⅰexpression was positively correrated with LVD,and lympha node metastasis.It is presumed that IGF-Ⅰcan promote the metastasis of lyphma node in tumor by inducing LVD expression and the detection of IGF-Ⅰin gastic cancer tissues will be significance for prediction of progonsis
引文
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[19]. Nakamura Y, Yasuoka H, Tsujimoto M ,et al .Importance of lymph vessel in gastric cancer: a prognostic indictor in general and predictor for lymph node metastasis in early stage cancer. [J].Clin Pathol,2006,59 (1): 77-82.
[20].邢光明,曲明阳,张桂平,等;血管和淋巴管生成及淋巴转移的关系.[J].中华普通外科杂志,2004,6,19( 6):361-63。
[21].Shida A;Fujioka S;Kobayashi K;et al, Expression of vascular endothelial growth factor (VEGF-C and -D )in gastric carcinoma. Int J Clin Oncol ,2006,11 (1):38-43
[22].张雅芳,赵玲辉,刘慧冬,等;人结肠癌和胃癌淋巴管形态结构的研究. [J].解剖科学进展,2003,9:8-12
[23].Saharinen P,Tammela T,Karkkainen M J,et a1.Lymphatic vasculature:development, molecular regulation and role in tumor metastasis and inflammation.[J].Trends Immunol, 2004, 25:387- 95.
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[2] Krishnan J,Kirkin V,Steffen A,et a1. Differential invivo and in vitro expression of vascular endothelialgrowth factor(VEGF)-C and VEGF-D in tumors and its relationship to lymphatic metastasis in immunocompetent rats. [ J].Cancer Res,2003,63:713- 22.
[3] Beasley NJ,Prevo R,Banerji S,et al. Intratumoral lymphangiogenesis and lymph node metastasis in head and neck cancer.[ J] .Cancer Res,2002,62:1315-20.
[4] Hachisuka T,Narikiyo M,Yamada Y,et al. High lymphatic vessel density correlates with over expression of VEGF-C in gastric cancer.[ J].Oncol Rep,2005,13 (4): 733-37.
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