玉米主要营养品质性状的QTL定位
摘要
淀粉、蛋白质和脂肪是普通玉米籽粒的主要营养成分。本研究通过SSR分子标记,利用农大108的F_2群体,构建遗传连锁图谱,进行玉米品质性状QTL定位。其研究结果如下:
1、亲本及F_2、F_(2∶3)群体、F_(2∶4)群体的品质表现
2007年重庆、2007年海南和2008年重庆三个环境下,亲本黄C的淀粉含量分别为72.3835%、74.0141%、73.5645%;178的淀粉含量分别为74.2520%、74.2329%、73.9470%;黄C蛋白质和油分的含量都略高于178。F_2、F_(2∶3)和F_(2∶4)群体的油分、蛋白质和淀粉含量均表现出超双亲分离。不同品质性状在各环境中都表现为连续分布,呈现多基因控制的遗传特点。
品质性状相关性分析表明,F_2、F_(2∶3)和F_(2∶4)群体的油分和蛋白质含量都呈正相关,相关达显著或极显著水平;油份和淀粉含量达极显著呈负相关;蛋白质和淀粉含量达极显著负相关。
F_2群体(2007重庆)、F_(2∶3)群体(2007海南)和F_(2∶4)群体(2008重庆)品质性状的方差分析表明:油分、蛋白质和淀粉含量的基因型间和环境间的差异都达到极显著水平,表明这3个品质性状受基因型影响的同时受环境影响。
2.遗传连锁图谱的构建
利用507对玉米SSR引物对亲本黄C和178进行多态性筛选,选用了其中91对差异引物用作F2群体检测。构建了包含15个连锁群的黄C×178的遗传连锁图,其中第1、2、4、7、8染色体上的标记被划分为两个连锁群;15个连锁群覆盖的标记位点为85个,总的遗传长度为769.4cM,标记间的平均距离为9.05cM。
3.品质性状QTL定位
以黄C×178 F2群体构建的遗传连锁图,结合2007年重庆、2007年海南和2008年重庆三个环境品质检测结果,运用区间作图法,以LOD=2.0,对品质性状进行全基因组QTLs扫描,共检测到34个品质性状QTLs。其中,油分含量检测到12个位点,解释性状表型变异的5.3%～17.7%;蛋白质含量检测到6个位点解释性状表型变异的5.3%～14.7%;淀粉含量检测到7个位点,解释性状表型变异的5.0%～10.4%。
Starch,protein and oil are main nutritional content in normal maize.In this paper,F_2 individuals derived from the cross between maize(Zea mays L·) inbreds Huang C and 178 were used for the mapping population.A maize genetic linkage map with simple sequence repeat(SSR) markers had been established to detect and map QTLs affecting nutritional quality.The main results were as follows:
1.Nutritional quality performances of mapping population:Parents,F_2,F_(2:3) and F_(2:4)
The quality traits under the three environmental conditions(2007 in Chongqing,2007 in Hainan and 2008 in Chongqing) were detected.Starch concentration of Huang C was 72.3835%,74.0141%, 73.5645%,while 178 is 74.2520%,74.2329%,73.9470%respectively.The protein and oil content of Huang C were better than that of 178 obviously.For the F_2,F_(2:3) and F_(2:4) population,Starch,protein and oil concentrations showed ultra-parent separation.
The nutritional quality traits showed continuous separation under those enviromental conditions, and these results suggested that the nutrition-related traits were controlled by multiple genes.
The correlation analysis of the quality traits indicated that the oil concentration of the F_2,F_(2:3) and F_(2:4) populations were positively significantly or highly significnatly correlated with their protein,while the oil concentration and the protein concentration were negatively highly significantly correlated with the starch concentration respectively.
The analysis of variance for the quality traits of F2 population(2007 in Chongqing),F_(2:3) population(2007 in Hainan) and F_(2:4) population(2008 in Chongqing) indicated that there existed highly significant difference for oil,protein and starch concentration of different genotypes and different environment,suggesting that these quality traits were affected both by genotypes and environmental conditions.
2.The construction of genetic linkage map
507 pairs of maize SSR primers were used to detect the polymorphism in parents Huang C and 178,of which 91pairs of primers were obtained.The genetic linkage map of Huang C×178 with 15 linkage groups had been established,in which the markers on the 1~(st),2~(nd),4~(th),7~(th) and the 8~(th) chromosome had been divided into two linkage groups.The 15 linkage groups covered 85 loci and spanned maize genome about 769.4cM,with an average interval is 9.05cM.
3.QTL of the quality traits
Based on the linkage map and and nutritional quality detected,34 QTLs controlling nutritional quality traits were identified by using QTL cartographer.We mapped 12 QTL associated with oil,explaining phenotypic variation for 5.3%～17.7%;6 QTL associated with protein,explaining phenotypic variation for 5.3%～14.7%;and 7 QTL associated with starch concentratios,explaining phenotypic variation for 5.0%～10.4%.
1、亲本及F_2、F_(2∶3)群体、F_(2∶4)群体的品质表现
2007年重庆、2007年海南和2008年重庆三个环境下,亲本黄C的淀粉含量分别为72.3835%、74.0141%、73.5645%;178的淀粉含量分别为74.2520%、74.2329%、73.9470%;黄C蛋白质和油分的含量都略高于178。F_2、F_(2∶3)和F_(2∶4)群体的油分、蛋白质和淀粉含量均表现出超双亲分离。不同品质性状在各环境中都表现为连续分布,呈现多基因控制的遗传特点。
品质性状相关性分析表明,F_2、F_(2∶3)和F_(2∶4)群体的油分和蛋白质含量都呈正相关,相关达显著或极显著水平;油份和淀粉含量达极显著呈负相关;蛋白质和淀粉含量达极显著负相关。
F_2群体(2007重庆)、F_(2∶3)群体(2007海南)和F_(2∶4)群体(2008重庆)品质性状的方差分析表明:油分、蛋白质和淀粉含量的基因型间和环境间的差异都达到极显著水平,表明这3个品质性状受基因型影响的同时受环境影响。
2.遗传连锁图谱的构建
利用507对玉米SSR引物对亲本黄C和178进行多态性筛选,选用了其中91对差异引物用作F2群体检测。构建了包含15个连锁群的黄C×178的遗传连锁图,其中第1、2、4、7、8染色体上的标记被划分为两个连锁群;15个连锁群覆盖的标记位点为85个,总的遗传长度为769.4cM,标记间的平均距离为9.05cM。
3.品质性状QTL定位
以黄C×178 F2群体构建的遗传连锁图,结合2007年重庆、2007年海南和2008年重庆三个环境品质检测结果,运用区间作图法,以LOD=2.0,对品质性状进行全基因组QTLs扫描,共检测到34个品质性状QTLs。其中,油分含量检测到12个位点,解释性状表型变异的5.3%～17.7%;蛋白质含量检测到6个位点解释性状表型变异的5.3%～14.7%;淀粉含量检测到7个位点,解释性状表型变异的5.0%～10.4%。
Starch,protein and oil are main nutritional content in normal maize.In this paper,F_2 individuals derived from the cross between maize(Zea mays L·) inbreds Huang C and 178 were used for the mapping population.A maize genetic linkage map with simple sequence repeat(SSR) markers had been established to detect and map QTLs affecting nutritional quality.The main results were as follows:
1.Nutritional quality performances of mapping population:Parents,F_2,F_(2:3) and F_(2:4)
The quality traits under the three environmental conditions(2007 in Chongqing,2007 in Hainan and 2008 in Chongqing) were detected.Starch concentration of Huang C was 72.3835%,74.0141%, 73.5645%,while 178 is 74.2520%,74.2329%,73.9470%respectively.The protein and oil content of Huang C were better than that of 178 obviously.For the F_2,F_(2:3) and F_(2:4) population,Starch,protein and oil concentrations showed ultra-parent separation.
The nutritional quality traits showed continuous separation under those enviromental conditions, and these results suggested that the nutrition-related traits were controlled by multiple genes.
The correlation analysis of the quality traits indicated that the oil concentration of the F_2,F_(2:3) and F_(2:4) populations were positively significantly or highly significnatly correlated with their protein,while the oil concentration and the protein concentration were negatively highly significantly correlated with the starch concentration respectively.
The analysis of variance for the quality traits of F2 population(2007 in Chongqing),F_(2:3) population(2007 in Hainan) and F_(2:4) population(2008 in Chongqing) indicated that there existed highly significant difference for oil,protein and starch concentration of different genotypes and different environment,suggesting that these quality traits were affected both by genotypes and environmental conditions.
2.The construction of genetic linkage map
507 pairs of maize SSR primers were used to detect the polymorphism in parents Huang C and 178,of which 91pairs of primers were obtained.The genetic linkage map of Huang C×178 with 15 linkage groups had been established,in which the markers on the 1~(st),2~(nd),4~(th),7~(th) and the 8~(th) chromosome had been divided into two linkage groups.The 15 linkage groups covered 85 loci and spanned maize genome about 769.4cM,with an average interval is 9.05cM.
3.QTL of the quality traits
Based on the linkage map and and nutritional quality detected,34 QTLs controlling nutritional quality traits were identified by using QTL cartographer.We mapped 12 QTL associated with oil,explaining phenotypic variation for 5.3%～17.7%;6 QTL associated with protein,explaining phenotypic variation for 5.3%～14.7%;and 7 QTL associated with starch concentratios,explaining phenotypic variation for 5.0%～10.4%.
引文
[1]M.B.Letchworth,R.J.Lambert.Pollen parent effects on oil,protein,and starch concentration in maize kemels(J).Crop Sci.,1998(38):363-367.
[2]Boyer CD,Hannah LC(2001) Kernel mutants of com.In:Hallauer AR(eds) Specialty corns.CRC,Boca Raton,FL,pp 1-31
[3]魏良明.普通玉米籽粒品质性状的遗传及其近红外测定方法的研究中国农业大学博士学位论文2003 1-10
[4]张雪梅,谭静,陈洪梅.高油玉米研究进展西南农业学报.2006.19卷增刊468-474
[5]刘纪麟主编.玉米育种学.北京:中国农业出版社(第二版).2002,1:227-237
[6]张晓芳.玉米种质资源品质性状的鉴定与评价.玉米科学,2006,14(1):18-20
[7]J.W.Dudley,M.A.Saghai Maroof,G.K.Rufener.Molecular marker information and selection of parents in corn breeding programs[J].Crop Sci.,1992(32):301-304.
[8]Mohamed M.ElRouby and L.H.Penny Variation and covariation in a high oil population of corn (Zea Mays L.) and their implications in selection[J].Crop Sci.,1967(7):216-219.
[9]Dudley R.J..Seventy-six generations of selection for oil and protein percentage in maize,Proc Int,Conference on Quantitative genetics,Ames,IA,1976,459-473.
[10]刘仁东,石德权,徐家舜.玉米籽粒含油量的配合力、方差成分和遗传力及其应用的研究.中国农业科学,1992.25(6):52-57
[11]宋同明,吴宏平,戴顺宏,等.互交群体中控制籽粒油分基因的遗传效应.北京农业大学学报,1991.17(1):15-22
[12]Alexander DE and Lambent RJ.Relationship of kernel oil content to yield in maize.Crop Sci.1968.8:272-274.
[13]Miller R.L.,Dudley JW,and Alexander DE.High intensity selection for percent oil in corn.Crop Sci.,1981,21:433-437.
[14]宋同明主编.高油玉米北京:北京农业大学出版社,1992.28,53-56
[15]范弘伟.高油玉米群体的杂种优势模式与高油授粉者选育[D].北京:中国农业大学,2001.
[16]王空军,张吉旺,刘鹏等.玉米不同品种粗蛋白质含量与产量的研究中国农业科学2005,38(5):916-921
[17]I.East,E.M.and D.F.Jones.Genetic studies on the protein content of maize.Genetics,19205:543-610
[18]汪黎明,等.1994.高赖氨酸玉米主要品质性状的配合力分析.作物学报,20(4):446-452
[19]李卫东,宋同明.对伊利诺高油(IHO C80)和亚里山索高油(Alexho C23)重组群体的遗传研究.作物学报,1991,6:470-475
[20]Frey,K.J.The inheritance of protein and certain of its components in maize.Agron.J.,1949.41:113-117
[21]Dudley,J W.Seventy-six Generations of Selection for Oil and Protein Percentage in Maize.Proc.Int,Conference on Quanlity Genetics,Ames,IA,1977,459-473
[22]Denic N.Genetics basis of storage protein synthesis in maize.In:Gottschalk W.(ed) Seed Protein.Junk Publishers,The Hague,1983
[23]Genter,C.F,Eheart J F,andi Linkous W NI.Oil and protein relationships between inbreds lines and their single cross progeny.Agrono.J.,1957,49(6):283-285
[24]Pollmer,W.G.,Klein D,and Dhillon B S.Inheritance of protein and yield of grain and stover in maize.Crop Sci.,1978,18:757-759
[25]Dudley,J.W.,and R.J.Lambert.Ninety generations of selection for oil and protein in maize.Maydica,1992,37:81-87
[26]李锦辉 高蛋白玉米的品质形成机理及关键技术研究.河南农业大学博士论文.2007.25-30
[27]Bhatia C R,Rabson R.Bioenergetic considerations in cereal breeding for protein improvement.Science1976,(194):1418-1421.
[28]Coerts P G.Correlation studies on sone grain quality and agronomic characters in the Collection ofmaize from Ancleam.Crop Improvement,1978,5(1):77-80.
[29]周琴,姜东,戴廷波等.不同基因型小麦籽粒蛋白质和淀粉积累与碳氮转运的关系.南京农业大学学报,2002,25(3):1-4.
[30]Aildson P D,Stephen C M,David S J,et al.Grain quality of Brazilian maize genotypes as influenced by nitrogen level.Crop Science,2005(45):1958-1964.
[31]王琳 不同环境条件下玉米籽粒的淀粉品质与主要农艺性状的遗传研究2006.30-35
[32]Letchworth MB,Lambert RJ1 Pollen parent effects on oil,protein and starch concentration in maize kernels.Crop Science,1998,38:363-367
[33]潘相文,金益,王立丰.玉米部分品质指标的遗传变异研究.东北农业大学学报,2002,33(4):331-336
[34]Goldman,I.L.et al.Quantitative trait loci influencing protein and starch concentration in Illinois Long Term Selection maize strains.Theo.App.Genet,1993,87:217-224
[35]Berke T G,Rocheford T R1 Quantitative trait loci for flowering,plant and ear height,and kernel traits in maize1 Crop Science,1995,35:1542-1549
[36]祁新,赵颖君.玉米品质性状的遗传模型分析[J],吉林农业科学,2001,26(3):32-35.
[37]刘祥久,姜敏,李哲等.玉米品质性状配合力研究[J],杂粮作物,2004,24(5):258-260,
[38]石海春.玉米籽粒主要品质性状的遗传分析,四川农业大学硕士论文,2003.45-50
[39]魏良明,戴景瑞,张义荣.玉米淀粉含量的杂种优势与基因效应分析.作物学报2005年7月 833-837
[40]周延清.DNA分子标记技术在植物研究中的应用.北京:化学工业出版社,2005
[41]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种.北京:科学出版社,2000
[42]Hamada H.A novel repeated element with Z-DNA—forming potential is widely found in evolutionarily diverse eukaryotic genomes.Proc Nail Acad Sci USA.1982,79:6465-6469
[43]Tautz D.Hyper variability of simple sequence as a general source for polymorphic DNA markers.Nucleic Acid Research.1989,17:6463-6471
[44]何平.真核生物中的微卫星及应用.遗传.1998,20(4):42-47
[45]张学勇,李大勇.小麦及其近亲基因组中的DNA重复序列研究进展.中国农业科学.2000,33(5):14-24
[46]Hazan J,Dubay C,Pankowiak M C et al.A genetic linkage map of human chromosome 20composed entirely of microsatellite markers.Genomics,1992,12:183-189
[47]Weissenbach J,Gyopay J,Dib C et al.A second generation linkage map of the human genome.Nature.1993,359:794-801
[48]Copeland N G,Jenkins N A,Gilbert D J et al.A genetic linkage map of the mouse:Current applications and future prospects.Science.1993,262:57-66
[49]杨留启,杨文鹏.SSR标记技术在玉米遗传育种中的应用.贵州农业科学.2008,36(2):5-7
[50]Dietrich W F,Miller J C.A comprehensive genitive map of the mouse genome.Nature.1996(380):149-152
[51]Dib C.A comprehensive genetic map of the human genome based on microsatellites.Nature.1996,380:152-154
[52]贺林.解码生命-人类基因组计划和后基因组计划.北京:科学出版社,2000
[53]况少青,傅刚,陈竺.微卫星标记分析在人类基因组多样性研究中的应用.国外医学遗传学分册.1997,20(1):5-8
[54]Saghai M A,Roof M A S,Biyashey R M,Yang G P et al.Extraordinarily polymorphic microsatellite DNA in barley species diversity,chromosomal locations,and population dynamics.Proc Natl Acad Sci.1994(91):5466-5470
[55]Wu K S,Tanksley S D.Abundance polymorphisms and genetic mapping of microsatellites in rice.Mole Gen Genet.1993,241:1225-235
[56]Senior M L.Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using act primer.Genome.1993,36:1884-889
[57]Bell C J,Ecker J H.Assignment of 30micrlsatellite loci to the linkage map of Arabidopsis.Genome.1994,9:137-144
[58]Chin E C L,Senior M L,Shu H et al.Maize simple repetitive DNA sequences:abundance and allele variation.Genome.1996,39:866-873
[59]Senior M L,E Chin,D Austin et al.Mapping simple sequence repeats in maize.Maize Genetics Cooperation Newsletter.1936,70:50-54
[60]Musket T G,Davis M,Polacco Met al.Maize probe bank,Maize genetics cooperation newsletter.1997,71:122-123
[61]陈翠霞,邢全华,粱春阳,等.南方玉米锈病抗病基因的定位及不同遗传背景对基因标记的比较分析.遗传学报,2003,30(4):341-344
[62]刘章雄,王守才,戴景瑞,等.玉米P25自交系抗锈病基因的遗传分析及SSR分子标记定位[J].遗传学报,2003,30(8):706-710
[63]汤继华,胡彦民,季洪强,等.玉米C型Cms育性恢复基因Rf4的SSR标记[J].河南农业大学学报,2001,35(1):1-3
[64]席章营,张书红,李新海等.一个新的抗玉米矮花叶病基因的发现及初步定位.作物学报2008,34(9):1494-1499
[65]Stam P,Zeven A C.Theoretical proportion of donor genomein nearisogenic lines of self2fertilizers bred by back2crossing[J].Euphytica,1981,30:227-238
[66]夏军红,郑用琏.玉米Rf 3近等基因系的分子标记辅助回交选育与效益分析[J].作物学报,2002,28(3):339-344
[67]宋敏,田清震,李新海,等.分子标记在优质蛋白玉米育种中的应用[J].新疆农业大学学报,2005,28(3):5-8
[68]Sun L H,Wang C M,Su C C et al.Mapping and Marker-assisted Selection of a Brown Planthopper Resistance Gene bph2 in Rice(Oryza sativa L.).Acta Genetica Sinica.2006,33(8):717-723
[69]阮成江,何祯祥.我国农作物QTL定位研究的现状和进展.植物学通报2003.20(1):10-22,
[70]Keightey P D,Blfield G.Detection of quantitative trait loci from frequency changes of marker alleles under selection.Genet Res.1993,62:195-203
[71]李维明,吴为人,卢浩然.检测作物数量性状基因与遗传连锁关系的方差分析法及应用.作物学报.1993,19(2):92-102
[72]Rodolphe F,Lefort M.A multi-marker model for detecting chromosomal segments displaying QTL activity.Genetics.1993,134:1277-1288
[73]Jansen R C.Interval mapping of multiple quantitative trait loci.Genetics.135:205-211
[74]Zeng Z B.Precision mapping of quantitative trait loci.Genetics.1994,136:1457-1468
[75]吴为人,李维明,卢浩然.基于最小二乘估计的数昔性状基因座的复合区间定位法.福建农业大学学报.1996,25(4):394-399
[76]Zhu J,Weir B S.Mixed model approaches for genetic quantitative traits.In:Chen L S,Ruan S G and Zhu J(eds) Advanced Topics in Biomathematics:Proceedings of international Conference on mathematical Biology.Singapore:World Scientific publishing Co.1998,321-330
[77]Moreno-Gonzalez J,Dudley J W,Lumber R J.A design of disequilibrium for percent oil in maize.Crop Sci.1975,15:840-843
[78]Kearsey M J,Farquhar A G.QTL analysis in plants:where are we now? Heredity,1998,80:137-142
[79]The Complex trait consortium.The nature and identification of quantitative trait loci:A community view.Nat Rev Genet,2003,4:911-916
[80]章元明.作物QTL定位方法研究进展.科学通报2006,51(19).2223-2231
[81]Goldman,I.L.,Rocheford,T.R.,Dudley,J.W.Molecular marker associated with maize kernel oil concentration in an Illinois high protein and Illinois low protein cross.Crop Sci.1994,34:908-915
[82].Mangolin,C.A.,C.L.De Souza Jr,A.A.F.Garcia,et al.Mapping QTLs for kernel oil content in a tropical maize population.Euphytica,2004,137(2):251-259
[83]杨文鹏,郑用琏,郑文婷等.玉米中一个高赖氨酸突变基因的定位及其与02基因聚合的效应.2004全国玉米种质扩增、改良、创新与分子育种学术会议论文集.北京:中国作物学会,2004,275-286
[84]Wassom,J.,J.Wong,E.Martinez,J.King,J.DeBaene,J.Hotchkiss,V.Mikkilenini,and T.Rocheford.2008.QTL associated with maize kernel oil,protein,and starch concentrations;kernelmass;and grain yield in Illinois High Oil×B73 backcrossderived lines.Crop Sci.2008.48:243-252.
[85]J.Zhang X.Q.Lu X.F.Song.Mapping quantitative trait loci for oil,starch,and protein concentrations in grain with high-oil maize by SSR markers Euphytica(2008) 162:335-344
[86]田国伟 氮胁迫条件下玉米籽粒和秸秆品质性状的QTL分析河南农业大学硕士学位论文2006
[87]Kim H O,Williams P C.Determination of starch and energy in feed grains by near-infrared reflectance spectroscopy.Journal of Agricultural Food Chemistry,1990,38:682-688.
[88]Orman B A,Schumann Jr R A.Comparison of near-infrared spectroscopy calibration methods for the prediction of protein,oil,and starch in maize grain.Journal of Agricultural Food Chemistry 1991,39:883-888.
[89]Campbell M R,Brumm T J,Glover D V.Whole grain amylose analysis in maize using near-infrared transmittance spectroscopy.Cereal Chemistry,1997,74(3):300-303.
[90]高文淑,张录达,王万军.应用付里叶变换近红外反射光谱法测定几种谷物籽粒中蛋白质的含量.北京农业大学学报,1990,16(增刊):72-79.
[91]李庆春,王文真,张玉良,贺微仙,杨金华,林澄菲,李宗智.近红外漫反射光谱分析法(NIRDRSA)在作物品质育种中的应用.作物学报,1992,18(3):235-240.
[92]舒庆尧,吴殿星,夏英武,高明尉,Anna Mc Clung.用近红外反射光谱测定小样本糙米粉的品 质性状.中国农业科学,1999,32(4):92-97.
[93]吴建国,石春海,张海珍,樊龙江.应用近红外反射光谱法整粒测定小样品油菜籽含油量的研究.作物学报,2002,28(3):421-425.
[94]曹干,谭中文,梁计南,龙永惠,周学秋.蔗汁品质成分的傅里叶变换近红外分析数学模型.中国农业科学,2003,36 C3):254-258.
[95]宋同明.脉冲核磁共振仪对作物种子含油量的快速测定,作物学报,1989,15(3):160.
[96]魏良明,姜海鹰,李军会,严衍禄,戴景瑞.玉米杂交种品质性状的近红外光谱分析技术研究,光谱学与光谱分析,2005,25(9):1404-1407
[97]白琪林,陈绍江,董晓玲,孟庆祥,严衍禄,戴景瑞.2004近红外漫反射光谱法测定玉米秸秆NDF与ADF含量,光谱与光谱分析24(11):1345-1347
[98]Frary A,Nesbitt T C,Grandillo S,van de Knaap E,Cong B,Hu J,Meller J,Elber R,Alport K,Tanksley S.Cloning and transgenic expression of fw-2:a quantitative traits locus key to the evolution of Science,2000,89:85-87
[99]J.W.Dudley,A.Djikhuizen,C.Paul,S.T.Coates,and T.R.Rocheford.Effects of Random Mating on Marker-QTL Associations in the Cross of the Illinois high protein×Illinois low protein.Maize Strains.Crop Science;Jul/Aug 2004;44,4;pg.1419-1428
[100]宋秀芳.玉米籽粒油分QTL定位及其效应分析.2003.中国农业大学博士研究生论文
[101]吕秀清.玉米籽粒油分QTL定位及相关性状的分析.2006.中国农业大学硕士研究生论文
[102]董永彬,李玉玲,牛素贞.利用三倍体胚乳遗传模型定位玉米籽粒淀粉含量QTL.遗传.2006:28(11)1401-1406
[103]盖钧镒.试验统计方法.北京:中国农业出版社.2000:137-173
[104]M A Saghai-Maroof,K M Soliman et al.Ribosomal DNA spacer-length polymorphisms in barley:Mendelian inheritance,chromosomal location,and population dynamics.Proc.Natl.Acad.Sci.984,81:8014-8018
[105]Van Ooijen J W,Voorrips R E.JoinMap 3.0,software for the calculation of genetic linkage maps.Plant Research International,Wageningen,the Netherlands.2001
[106]Kosambi D D.The estimation of map distance from recombination values.Ann.Eugen.1944,12:172-175
[107]Voorrips R E MapChart 2.2:Software for the graphical presentation of linkage maps and QTLs.Plant Research International,Wageningen,the Netherlands.2006
[108]Ooijen van J W.MapQTL 5.0 Software for the mapping of quantitative trait loci in experimental population.Plant Research International,Wageningen,the Netherlands.2002
[109]Flint-Garcia S A,J.M.Thornsberry and E.S.Buckler.Structure of linkage disequilibrium in plants.Annu.Rev.Plant Biol.2003,54:357-74
[110]Stuber C W,M D Edwards and J F Wendel.Molecular marker-facilitated investigations of quantitative trait loci in maize,Factors influencing yield and its component traits.Crop Science.1987,27:639-648
[111]Emerson R A,Farser A C,Farser A C.Summary of linkage studies in maize.Comell University,Agricultural Experiment Station,Memoir.1935,180:1-83
[112]Botstein D,Skolnick M,Skolnick M.Construction of a genetic linkage map in man using restriction fragment length polymorphism.American Journal of Human Genetics.1980,32:314-331
[113]李玉玲,董永彬,牛素贞.爆裂玉米3个膨爆特性的非条件和条件QTL分析.分子植物育种.2006.Vol.4.NO3,372-380
[114]万素琴,邵艳华,袁有禄,章元明.F2:3设计全基因组标记的Bayesian分析.作物学报.2007,33(12):1943-1948
[115]Lander E.S.,and Botstein D.,1989,Mapping Mendelian factors underlying quantitative traits using RFLP likage maps,Genet.,121:185-199
[116]Mackay T.F.C.,2001,The genetic architecture of quantitative traits,Annu.Rev.Genet.,35:303-339
[117]Melchinger A.E.,Utz H.E,and Schon C.C.,1998,Quantitative trait locus(QTL) mapping using different testers and independent population samples in maize reveals low power of QTL detection and large bias in estimates of QTL effects,Genet.,149(1):383.403
[118]宋宪亮,孙学振,张人真.偏分离及对植物遗传作图的影响.农业生物技术学报.2006,14(2)286-292
[119]Charlesworth B.Driving genes and chromosomes(J).Nature,1988,332:394-395
[120]Sibov S T,de Souza Jr C L,Garcia A A F,Garcia A F,Silva AR,Mangolin C A,Benchimol L La nd de Souza A P.Molecular mappingi n tropical maize(Zea mays L.) using microsatellite markers.1.Map construction and localization of locishowing distorted segregation(J).Hereditas,2003,139:96-106
[121]Cloutier,S,Cappadocia,M and Landry,B S Analysis of RFLP mapping inaccuracy in Brassica napus L(J).Genetics,1997,95:83-91
[122]Smith J S C,Chin E C L,Shu H,Smith O S,Wall S J,Senior ML,Mitchell S E,Kresovich S and Ziegle J.An evaluation of the utility of SSR loci as molecular markers in maize(Zea mays L.):Comparisons with data from RFLP and pedigree(J).Theoretical Applied Genetics,1997,95:163-173
[123]Liedl B,and Anderson N O.Reproductive barriers:Identification,uses and circumvention(J).Plant Breed Review.1993,11:11-54
[124]Karkkainen K,Koski V and Savolainen.Geographical variationin inbreeding depression in Scots pine(J).Evolution,1996,50:111-119
[125]Kreike D D and Stiekema W J.Reduced recombination and distorted segregation in a Solanum tuberosum(2x)×S.spegazzinii(2x) hybrid(J).Genome,1997,40:180-187
[126]Perfectti F and Pascual L.Segregation distortion of isozyme loci in cherimoya(Annona cherimola Mill)(J).Theoretical Applied Genetics,1996,93:440-446
[127]ZHANG Fan,WAN Xue-Qin and PAN Guang-Tang.Genetic analysis of segregation distortion of molecular markers in maize F2 population.ACTA AGRONOMICA SINICA.2006,32(9):1391-1396
[128]汤华,严建兵,黄益勤,郑用琏,李建生.玉米5个农艺性状的QTL定位.遗传学报.2005,32(2):203-309
[129]Tuberosa R,Salvi S,Sanguineti M,Landi P,Maccaferri M,ContiS.Mapping QTLs regulating morphophysiological traits and yield:case studies,shortcomings and perspectives in drought-stressed maize.Ann Bot,2002,89(7):941-963.
[130]Huang N,Angeles E R,Domingo J,Magpantay G,Singh S,Zhang G,Kumaravadivel N,Bennett J,Khush G S.Pyramiding of bacterial blight resistance genes inr ice:Marker-assisted selection using RFLP and PCR.Theor.Appl.Genet.1997,95(3):313-320
[131]Chen S,Lin X H,Xu C G,Zhang Q F.Improvement of bacteria blight resistance of 'Minghui 63',an elite restorer line of hybrid rice,by molecular marker-assisted selection.Crop Sci.2000,4(1):239-244
[132]张志明,赵茂俊,荣廷昭,潘光堂.玉米SSR连锁图谱构建与株高及穗位高QTL定位.作物学报.2007,33(2):341-344
[133]尹小燕,王庆华,王飞等.玉米大斑病抗性基因Ht2的精细定位.科学通报.2002,23:1811-1814
[134]高伟,陈晓,库丽霞等.玉米类LFY基因的克隆及其在不同光周期条件下的表达.作物学报.2006.8:1256-1260
[135]Tanksley SD,Ganal MW,Prince JP,et al.High density molecular linkage map of the tomato and potato genomes.Genetics,1992,132:1141-1160
[2]Boyer CD,Hannah LC(2001) Kernel mutants of com.In:Hallauer AR(eds) Specialty corns.CRC,Boca Raton,FL,pp 1-31
[3]魏良明.普通玉米籽粒品质性状的遗传及其近红外测定方法的研究中国农业大学博士学位论文2003 1-10
[4]张雪梅,谭静,陈洪梅.高油玉米研究进展西南农业学报.2006.19卷增刊468-474
[5]刘纪麟主编.玉米育种学.北京:中国农业出版社(第二版).2002,1:227-237
[6]张晓芳.玉米种质资源品质性状的鉴定与评价.玉米科学,2006,14(1):18-20
[7]J.W.Dudley,M.A.Saghai Maroof,G.K.Rufener.Molecular marker information and selection of parents in corn breeding programs[J].Crop Sci.,1992(32):301-304.
[8]Mohamed M.ElRouby and L.H.Penny Variation and covariation in a high oil population of corn (Zea Mays L.) and their implications in selection[J].Crop Sci.,1967(7):216-219.
[9]Dudley R.J..Seventy-six generations of selection for oil and protein percentage in maize,Proc Int,Conference on Quantitative genetics,Ames,IA,1976,459-473.
[10]刘仁东,石德权,徐家舜.玉米籽粒含油量的配合力、方差成分和遗传力及其应用的研究.中国农业科学,1992.25(6):52-57
[11]宋同明,吴宏平,戴顺宏,等.互交群体中控制籽粒油分基因的遗传效应.北京农业大学学报,1991.17(1):15-22
[12]Alexander DE and Lambent RJ.Relationship of kernel oil content to yield in maize.Crop Sci.1968.8:272-274.
[13]Miller R.L.,Dudley JW,and Alexander DE.High intensity selection for percent oil in corn.Crop Sci.,1981,21:433-437.
[14]宋同明主编.高油玉米北京:北京农业大学出版社,1992.28,53-56
[15]范弘伟.高油玉米群体的杂种优势模式与高油授粉者选育[D].北京:中国农业大学,2001.
[16]王空军,张吉旺,刘鹏等.玉米不同品种粗蛋白质含量与产量的研究中国农业科学2005,38(5):916-921
[17]I.East,E.M.and D.F.Jones.Genetic studies on the protein content of maize.Genetics,19205:543-610
[18]汪黎明,等.1994.高赖氨酸玉米主要品质性状的配合力分析.作物学报,20(4):446-452
[19]李卫东,宋同明.对伊利诺高油(IHO C80)和亚里山索高油(Alexho C23)重组群体的遗传研究.作物学报,1991,6:470-475
[20]Frey,K.J.The inheritance of protein and certain of its components in maize.Agron.J.,1949.41:113-117
[21]Dudley,J W.Seventy-six Generations of Selection for Oil and Protein Percentage in Maize.Proc.Int,Conference on Quanlity Genetics,Ames,IA,1977,459-473
[22]Denic N.Genetics basis of storage protein synthesis in maize.In:Gottschalk W.(ed) Seed Protein.Junk Publishers,The Hague,1983
[23]Genter,C.F,Eheart J F,andi Linkous W NI.Oil and protein relationships between inbreds lines and their single cross progeny.Agrono.J.,1957,49(6):283-285
[24]Pollmer,W.G.,Klein D,and Dhillon B S.Inheritance of protein and yield of grain and stover in maize.Crop Sci.,1978,18:757-759
[25]Dudley,J.W.,and R.J.Lambert.Ninety generations of selection for oil and protein in maize.Maydica,1992,37:81-87
[26]李锦辉 高蛋白玉米的品质形成机理及关键技术研究.河南农业大学博士论文.2007.25-30
[27]Bhatia C R,Rabson R.Bioenergetic considerations in cereal breeding for protein improvement.Science1976,(194):1418-1421.
[28]Coerts P G.Correlation studies on sone grain quality and agronomic characters in the Collection ofmaize from Ancleam.Crop Improvement,1978,5(1):77-80.
[29]周琴,姜东,戴廷波等.不同基因型小麦籽粒蛋白质和淀粉积累与碳氮转运的关系.南京农业大学学报,2002,25(3):1-4.
[30]Aildson P D,Stephen C M,David S J,et al.Grain quality of Brazilian maize genotypes as influenced by nitrogen level.Crop Science,2005(45):1958-1964.
[31]王琳 不同环境条件下玉米籽粒的淀粉品质与主要农艺性状的遗传研究2006.30-35
[32]Letchworth MB,Lambert RJ1 Pollen parent effects on oil,protein and starch concentration in maize kernels.Crop Science,1998,38:363-367
[33]潘相文,金益,王立丰.玉米部分品质指标的遗传变异研究.东北农业大学学报,2002,33(4):331-336
[34]Goldman,I.L.et al.Quantitative trait loci influencing protein and starch concentration in Illinois Long Term Selection maize strains.Theo.App.Genet,1993,87:217-224
[35]Berke T G,Rocheford T R1 Quantitative trait loci for flowering,plant and ear height,and kernel traits in maize1 Crop Science,1995,35:1542-1549
[36]祁新,赵颖君.玉米品质性状的遗传模型分析[J],吉林农业科学,2001,26(3):32-35.
[37]刘祥久,姜敏,李哲等.玉米品质性状配合力研究[J],杂粮作物,2004,24(5):258-260,
[38]石海春.玉米籽粒主要品质性状的遗传分析,四川农业大学硕士论文,2003.45-50
[39]魏良明,戴景瑞,张义荣.玉米淀粉含量的杂种优势与基因效应分析.作物学报2005年7月 833-837
[40]周延清.DNA分子标记技术在植物研究中的应用.北京:化学工业出版社,2005
[41]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种.北京:科学出版社,2000
[42]Hamada H.A novel repeated element with Z-DNA—forming potential is widely found in evolutionarily diverse eukaryotic genomes.Proc Nail Acad Sci USA.1982,79:6465-6469
[43]Tautz D.Hyper variability of simple sequence as a general source for polymorphic DNA markers.Nucleic Acid Research.1989,17:6463-6471
[44]何平.真核生物中的微卫星及应用.遗传.1998,20(4):42-47
[45]张学勇,李大勇.小麦及其近亲基因组中的DNA重复序列研究进展.中国农业科学.2000,33(5):14-24
[46]Hazan J,Dubay C,Pankowiak M C et al.A genetic linkage map of human chromosome 20composed entirely of microsatellite markers.Genomics,1992,12:183-189
[47]Weissenbach J,Gyopay J,Dib C et al.A second generation linkage map of the human genome.Nature.1993,359:794-801
[48]Copeland N G,Jenkins N A,Gilbert D J et al.A genetic linkage map of the mouse:Current applications and future prospects.Science.1993,262:57-66
[49]杨留启,杨文鹏.SSR标记技术在玉米遗传育种中的应用.贵州农业科学.2008,36(2):5-7
[50]Dietrich W F,Miller J C.A comprehensive genitive map of the mouse genome.Nature.1996(380):149-152
[51]Dib C.A comprehensive genetic map of the human genome based on microsatellites.Nature.1996,380:152-154
[52]贺林.解码生命-人类基因组计划和后基因组计划.北京:科学出版社,2000
[53]况少青,傅刚,陈竺.微卫星标记分析在人类基因组多样性研究中的应用.国外医学遗传学分册.1997,20(1):5-8
[54]Saghai M A,Roof M A S,Biyashey R M,Yang G P et al.Extraordinarily polymorphic microsatellite DNA in barley species diversity,chromosomal locations,and population dynamics.Proc Natl Acad Sci.1994(91):5466-5470
[55]Wu K S,Tanksley S D.Abundance polymorphisms and genetic mapping of microsatellites in rice.Mole Gen Genet.1993,241:1225-235
[56]Senior M L.Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using act primer.Genome.1993,36:1884-889
[57]Bell C J,Ecker J H.Assignment of 30micrlsatellite loci to the linkage map of Arabidopsis.Genome.1994,9:137-144
[58]Chin E C L,Senior M L,Shu H et al.Maize simple repetitive DNA sequences:abundance and allele variation.Genome.1996,39:866-873
[59]Senior M L,E Chin,D Austin et al.Mapping simple sequence repeats in maize.Maize Genetics Cooperation Newsletter.1936,70:50-54
[60]Musket T G,Davis M,Polacco Met al.Maize probe bank,Maize genetics cooperation newsletter.1997,71:122-123
[61]陈翠霞,邢全华,粱春阳,等.南方玉米锈病抗病基因的定位及不同遗传背景对基因标记的比较分析.遗传学报,2003,30(4):341-344
[62]刘章雄,王守才,戴景瑞,等.玉米P25自交系抗锈病基因的遗传分析及SSR分子标记定位[J].遗传学报,2003,30(8):706-710
[63]汤继华,胡彦民,季洪强,等.玉米C型Cms育性恢复基因Rf4的SSR标记[J].河南农业大学学报,2001,35(1):1-3
[64]席章营,张书红,李新海等.一个新的抗玉米矮花叶病基因的发现及初步定位.作物学报2008,34(9):1494-1499
[65]Stam P,Zeven A C.Theoretical proportion of donor genomein nearisogenic lines of self2fertilizers bred by back2crossing[J].Euphytica,1981,30:227-238
[66]夏军红,郑用琏.玉米Rf 3近等基因系的分子标记辅助回交选育与效益分析[J].作物学报,2002,28(3):339-344
[67]宋敏,田清震,李新海,等.分子标记在优质蛋白玉米育种中的应用[J].新疆农业大学学报,2005,28(3):5-8
[68]Sun L H,Wang C M,Su C C et al.Mapping and Marker-assisted Selection of a Brown Planthopper Resistance Gene bph2 in Rice(Oryza sativa L.).Acta Genetica Sinica.2006,33(8):717-723
[69]阮成江,何祯祥.我国农作物QTL定位研究的现状和进展.植物学通报2003.20(1):10-22,
[70]Keightey P D,Blfield G.Detection of quantitative trait loci from frequency changes of marker alleles under selection.Genet Res.1993,62:195-203
[71]李维明,吴为人,卢浩然.检测作物数量性状基因与遗传连锁关系的方差分析法及应用.作物学报.1993,19(2):92-102
[72]Rodolphe F,Lefort M.A multi-marker model for detecting chromosomal segments displaying QTL activity.Genetics.1993,134:1277-1288
[73]Jansen R C.Interval mapping of multiple quantitative trait loci.Genetics.135:205-211
[74]Zeng Z B.Precision mapping of quantitative trait loci.Genetics.1994,136:1457-1468
[75]吴为人,李维明,卢浩然.基于最小二乘估计的数昔性状基因座的复合区间定位法.福建农业大学学报.1996,25(4):394-399
[76]Zhu J,Weir B S.Mixed model approaches for genetic quantitative traits.In:Chen L S,Ruan S G and Zhu J(eds) Advanced Topics in Biomathematics:Proceedings of international Conference on mathematical Biology.Singapore:World Scientific publishing Co.1998,321-330
[77]Moreno-Gonzalez J,Dudley J W,Lumber R J.A design of disequilibrium for percent oil in maize.Crop Sci.1975,15:840-843
[78]Kearsey M J,Farquhar A G.QTL analysis in plants:where are we now? Heredity,1998,80:137-142
[79]The Complex trait consortium.The nature and identification of quantitative trait loci:A community view.Nat Rev Genet,2003,4:911-916
[80]章元明.作物QTL定位方法研究进展.科学通报2006,51(19).2223-2231
[81]Goldman,I.L.,Rocheford,T.R.,Dudley,J.W.Molecular marker associated with maize kernel oil concentration in an Illinois high protein and Illinois low protein cross.Crop Sci.1994,34:908-915
[82].Mangolin,C.A.,C.L.De Souza Jr,A.A.F.Garcia,et al.Mapping QTLs for kernel oil content in a tropical maize population.Euphytica,2004,137(2):251-259
[83]杨文鹏,郑用琏,郑文婷等.玉米中一个高赖氨酸突变基因的定位及其与02基因聚合的效应.2004全国玉米种质扩增、改良、创新与分子育种学术会议论文集.北京:中国作物学会,2004,275-286
[84]Wassom,J.,J.Wong,E.Martinez,J.King,J.DeBaene,J.Hotchkiss,V.Mikkilenini,and T.Rocheford.2008.QTL associated with maize kernel oil,protein,and starch concentrations;kernelmass;and grain yield in Illinois High Oil×B73 backcrossderived lines.Crop Sci.2008.48:243-252.
[85]J.Zhang X.Q.Lu X.F.Song.Mapping quantitative trait loci for oil,starch,and protein concentrations in grain with high-oil maize by SSR markers Euphytica(2008) 162:335-344
[86]田国伟 氮胁迫条件下玉米籽粒和秸秆品质性状的QTL分析河南农业大学硕士学位论文2006
[87]Kim H O,Williams P C.Determination of starch and energy in feed grains by near-infrared reflectance spectroscopy.Journal of Agricultural Food Chemistry,1990,38:682-688.
[88]Orman B A,Schumann Jr R A.Comparison of near-infrared spectroscopy calibration methods for the prediction of protein,oil,and starch in maize grain.Journal of Agricultural Food Chemistry 1991,39:883-888.
[89]Campbell M R,Brumm T J,Glover D V.Whole grain amylose analysis in maize using near-infrared transmittance spectroscopy.Cereal Chemistry,1997,74(3):300-303.
[90]高文淑,张录达,王万军.应用付里叶变换近红外反射光谱法测定几种谷物籽粒中蛋白质的含量.北京农业大学学报,1990,16(增刊):72-79.
[91]李庆春,王文真,张玉良,贺微仙,杨金华,林澄菲,李宗智.近红外漫反射光谱分析法(NIRDRSA)在作物品质育种中的应用.作物学报,1992,18(3):235-240.
[92]舒庆尧,吴殿星,夏英武,高明尉,Anna Mc Clung.用近红外反射光谱测定小样本糙米粉的品 质性状.中国农业科学,1999,32(4):92-97.
[93]吴建国,石春海,张海珍,樊龙江.应用近红外反射光谱法整粒测定小样品油菜籽含油量的研究.作物学报,2002,28(3):421-425.
[94]曹干,谭中文,梁计南,龙永惠,周学秋.蔗汁品质成分的傅里叶变换近红外分析数学模型.中国农业科学,2003,36 C3):254-258.
[95]宋同明.脉冲核磁共振仪对作物种子含油量的快速测定,作物学报,1989,15(3):160.
[96]魏良明,姜海鹰,李军会,严衍禄,戴景瑞.玉米杂交种品质性状的近红外光谱分析技术研究,光谱学与光谱分析,2005,25(9):1404-1407
[97]白琪林,陈绍江,董晓玲,孟庆祥,严衍禄,戴景瑞.2004近红外漫反射光谱法测定玉米秸秆NDF与ADF含量,光谱与光谱分析24(11):1345-1347
[98]Frary A,Nesbitt T C,Grandillo S,van de Knaap E,Cong B,Hu J,Meller J,Elber R,Alport K,Tanksley S.Cloning and transgenic expression of fw-2:a quantitative traits locus key to the evolution of Science,2000,89:85-87
[99]J.W.Dudley,A.Djikhuizen,C.Paul,S.T.Coates,and T.R.Rocheford.Effects of Random Mating on Marker-QTL Associations in the Cross of the Illinois high protein×Illinois low protein.Maize Strains.Crop Science;Jul/Aug 2004;44,4;pg.1419-1428
[100]宋秀芳.玉米籽粒油分QTL定位及其效应分析.2003.中国农业大学博士研究生论文
[101]吕秀清.玉米籽粒油分QTL定位及相关性状的分析.2006.中国农业大学硕士研究生论文
[102]董永彬,李玉玲,牛素贞.利用三倍体胚乳遗传模型定位玉米籽粒淀粉含量QTL.遗传.2006:28(11)1401-1406
[103]盖钧镒.试验统计方法.北京:中国农业出版社.2000:137-173
[104]M A Saghai-Maroof,K M Soliman et al.Ribosomal DNA spacer-length polymorphisms in barley:Mendelian inheritance,chromosomal location,and population dynamics.Proc.Natl.Acad.Sci.984,81:8014-8018
[105]Van Ooijen J W,Voorrips R E.JoinMap 3.0,software for the calculation of genetic linkage maps.Plant Research International,Wageningen,the Netherlands.2001
[106]Kosambi D D.The estimation of map distance from recombination values.Ann.Eugen.1944,12:172-175
[107]Voorrips R E MapChart 2.2:Software for the graphical presentation of linkage maps and QTLs.Plant Research International,Wageningen,the Netherlands.2006
[108]Ooijen van J W.MapQTL 5.0 Software for the mapping of quantitative trait loci in experimental population.Plant Research International,Wageningen,the Netherlands.2002
[109]Flint-Garcia S A,J.M.Thornsberry and E.S.Buckler.Structure of linkage disequilibrium in plants.Annu.Rev.Plant Biol.2003,54:357-74
[110]Stuber C W,M D Edwards and J F Wendel.Molecular marker-facilitated investigations of quantitative trait loci in maize,Factors influencing yield and its component traits.Crop Science.1987,27:639-648
[111]Emerson R A,Farser A C,Farser A C.Summary of linkage studies in maize.Comell University,Agricultural Experiment Station,Memoir.1935,180:1-83
[112]Botstein D,Skolnick M,Skolnick M.Construction of a genetic linkage map in man using restriction fragment length polymorphism.American Journal of Human Genetics.1980,32:314-331
[113]李玉玲,董永彬,牛素贞.爆裂玉米3个膨爆特性的非条件和条件QTL分析.分子植物育种.2006.Vol.4.NO3,372-380
[114]万素琴,邵艳华,袁有禄,章元明.F2:3设计全基因组标记的Bayesian分析.作物学报.2007,33(12):1943-1948
[115]Lander E.S.,and Botstein D.,1989,Mapping Mendelian factors underlying quantitative traits using RFLP likage maps,Genet.,121:185-199
[116]Mackay T.F.C.,2001,The genetic architecture of quantitative traits,Annu.Rev.Genet.,35:303-339
[117]Melchinger A.E.,Utz H.E,and Schon C.C.,1998,Quantitative trait locus(QTL) mapping using different testers and independent population samples in maize reveals low power of QTL detection and large bias in estimates of QTL effects,Genet.,149(1):383.403
[118]宋宪亮,孙学振,张人真.偏分离及对植物遗传作图的影响.农业生物技术学报.2006,14(2)286-292
[119]Charlesworth B.Driving genes and chromosomes(J).Nature,1988,332:394-395
[120]Sibov S T,de Souza Jr C L,Garcia A A F,Garcia A F,Silva AR,Mangolin C A,Benchimol L La nd de Souza A P.Molecular mappingi n tropical maize(Zea mays L.) using microsatellite markers.1.Map construction and localization of locishowing distorted segregation(J).Hereditas,2003,139:96-106
[121]Cloutier,S,Cappadocia,M and Landry,B S Analysis of RFLP mapping inaccuracy in Brassica napus L(J).Genetics,1997,95:83-91
[122]Smith J S C,Chin E C L,Shu H,Smith O S,Wall S J,Senior ML,Mitchell S E,Kresovich S and Ziegle J.An evaluation of the utility of SSR loci as molecular markers in maize(Zea mays L.):Comparisons with data from RFLP and pedigree(J).Theoretical Applied Genetics,1997,95:163-173
[123]Liedl B,and Anderson N O.Reproductive barriers:Identification,uses and circumvention(J).Plant Breed Review.1993,11:11-54
[124]Karkkainen K,Koski V and Savolainen.Geographical variationin inbreeding depression in Scots pine(J).Evolution,1996,50:111-119
[125]Kreike D D and Stiekema W J.Reduced recombination and distorted segregation in a Solanum tuberosum(2x)×S.spegazzinii(2x) hybrid(J).Genome,1997,40:180-187
[126]Perfectti F and Pascual L.Segregation distortion of isozyme loci in cherimoya(Annona cherimola Mill)(J).Theoretical Applied Genetics,1996,93:440-446
[127]ZHANG Fan,WAN Xue-Qin and PAN Guang-Tang.Genetic analysis of segregation distortion of molecular markers in maize F2 population.ACTA AGRONOMICA SINICA.2006,32(9):1391-1396
[128]汤华,严建兵,黄益勤,郑用琏,李建生.玉米5个农艺性状的QTL定位.遗传学报.2005,32(2):203-309
[129]Tuberosa R,Salvi S,Sanguineti M,Landi P,Maccaferri M,ContiS.Mapping QTLs regulating morphophysiological traits and yield:case studies,shortcomings and perspectives in drought-stressed maize.Ann Bot,2002,89(7):941-963.
[130]Huang N,Angeles E R,Domingo J,Magpantay G,Singh S,Zhang G,Kumaravadivel N,Bennett J,Khush G S.Pyramiding of bacterial blight resistance genes inr ice:Marker-assisted selection using RFLP and PCR.Theor.Appl.Genet.1997,95(3):313-320
[131]Chen S,Lin X H,Xu C G,Zhang Q F.Improvement of bacteria blight resistance of 'Minghui 63',an elite restorer line of hybrid rice,by molecular marker-assisted selection.Crop Sci.2000,4(1):239-244
[132]张志明,赵茂俊,荣廷昭,潘光堂.玉米SSR连锁图谱构建与株高及穗位高QTL定位.作物学报.2007,33(2):341-344
[133]尹小燕,王庆华,王飞等.玉米大斑病抗性基因Ht2的精细定位.科学通报.2002,23:1811-1814
[134]高伟,陈晓,库丽霞等.玉米类LFY基因的克隆及其在不同光周期条件下的表达.作物学报.2006.8:1256-1260
[135]Tanksley SD,Ganal MW,Prince JP,et al.High density molecular linkage map of the tomato and potato genomes.Genetics,1992,132:1141-1160