人食管鳞癌组织RIZ1基因启动子区甲基化状态的研究
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摘要
目的：
检测人食管鳞癌组织及其相应癌旁组织、正常组织中RIZ1基因启动子区CpG岛的甲基化状态,并探讨其与人食管鳞癌临床及病理特征的关系。
方法：
采集人食管鳞癌患者的癌组织、相应癌旁组织及正常组织标本,收集患者的临床与病理资料。
应用甲基化特异性聚合酶链反应(methylation-specific PCR, MSP)技术检测47例食管鳞癌患者的癌组织,相应癌旁组织及正常组织中RIZ1基因启动子区CpG岛的甲基化状态。结合该47例患者的性别、年龄、家族史、肿瘤分化程度、肿瘤TNM分期及临床分期等临床与病理资料,分析RIZ1基因启动子区CpG岛的甲基化情况与患者临床及病理特征的关系。
结果：
47例食管鳞癌患者的癌组织、相应癌旁组织及正常组织中RIZ1基因启动子区CpG岛发生甲基化的例数分别为：26例、3例和0例,甲基化阳性率分别为55.3%、6.4%和0.0%。食管鳞癌组织的甲基化发生率显著高于癌旁组织及正常组织,差异有统计学意义(P<0.01),癌旁组织与正常组织的甲基化发生率相比较,差异无统计学意义(P>0.05)。RIZ1基因启动子区CpG岛甲基化情况与该47例食管癌患者的性别、年龄、家族史、肿瘤分化程度、肿瘤TNM分期及临床分期情况关系不明显,各组内比较差异均无统计学意义(各组内P均>0.05)。
结论：
RIZ1基因启动子区在人食管鳞癌组织中的高甲基化,说明其与食管鳞癌的发生关系密切,可能是人食管鳞癌在肿瘤形成过程中的一个重要促成因素。RIZ1基因启动子区CpG岛甲基化可能是食管鳞癌发生的早期事件之一,为食管鳞癌的早期筛选及诊断提供了一个潜在的生物学指标。
Objective:
To detect the CpG Islands which in promoter region methylation status of RIZ1 gene in the human esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue. And analyze their relationship with the clinical and pathological features of the human esophageal squamous carcinoma.
Methods:
The human esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue specimens and their clinical and pathological data were collected.
Methylation-specific PCR(MSP) was used to investigate the CpG Islands which in promoter region methylation status of RIZ1 gene in the carcinoma tissues, the adjacent tissues and the normal tissues from 47 esophageal squamous cell carcinoma patients. The relationship between the CpG Islands which in promoter region methylation status of RIZ1 gene and the clinical and pathological features of the patients was analyzed combined with the related data such as gender, age, family history, cell differentiation of tumor, TNM classification of tumor and clinical stage of the 47 esophageal squamous cell carcinoma patients.
Results:
The cases of CpG Islands which in promoter region methylation of RIZ1 gene were found 26,3,0 in the esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue specimens of 47 esophageal squamous cell carcinoma patients respectively. The positivity rate of promoter region methylation of RIZ1 gene was significantly higher in esophageal squamous carcinoma tissue than that in adjacent tissue and the normal tissue (55.3%vs.6.4%and 0.0%, P<0.01), but no significant difference was detected between the adjacent tissue and the normal tissue (P>0.05). We found that the CpG Islands which in promoter region methylation status of RIZ1 gene in the human esophageal squamous carcinoma had no obviously relationship with the data of gender, age, family history, cell differentiation of tumor, TNM classification of tumor and clinical stage (P>0.05, respectively)
Conclusion:
Promoter hypermethylation of RIZ1 gene is present in the human esophageal squamous carcinoma, suggesting that the promoter hypermethylation of RIZ1 gene was associated with the tumorigenesis of human esophageal squamous carcinoma, it may be a significant contributing factor to tumour development in human esophageal squamous carcinoma. The CpG Islands which in promoter region methylation of RIZ1 gene may be one of the earlist events in the occurrence of human esophageal squamous carcinoma, it may provide help in the early detection of esophageal squamous carcinoma.
检测人食管鳞癌组织及其相应癌旁组织、正常组织中RIZ1基因启动子区CpG岛的甲基化状态,并探讨其与人食管鳞癌临床及病理特征的关系。
方法：
采集人食管鳞癌患者的癌组织、相应癌旁组织及正常组织标本,收集患者的临床与病理资料。
应用甲基化特异性聚合酶链反应(methylation-specific PCR, MSP)技术检测47例食管鳞癌患者的癌组织,相应癌旁组织及正常组织中RIZ1基因启动子区CpG岛的甲基化状态。结合该47例患者的性别、年龄、家族史、肿瘤分化程度、肿瘤TNM分期及临床分期等临床与病理资料,分析RIZ1基因启动子区CpG岛的甲基化情况与患者临床及病理特征的关系。
结果：
47例食管鳞癌患者的癌组织、相应癌旁组织及正常组织中RIZ1基因启动子区CpG岛发生甲基化的例数分别为：26例、3例和0例,甲基化阳性率分别为55.3%、6.4%和0.0%。食管鳞癌组织的甲基化发生率显著高于癌旁组织及正常组织,差异有统计学意义(P<0.01),癌旁组织与正常组织的甲基化发生率相比较,差异无统计学意义(P>0.05)。RIZ1基因启动子区CpG岛甲基化情况与该47例食管癌患者的性别、年龄、家族史、肿瘤分化程度、肿瘤TNM分期及临床分期情况关系不明显,各组内比较差异均无统计学意义(各组内P均>0.05)。
结论：
RIZ1基因启动子区在人食管鳞癌组织中的高甲基化,说明其与食管鳞癌的发生关系密切,可能是人食管鳞癌在肿瘤形成过程中的一个重要促成因素。RIZ1基因启动子区CpG岛甲基化可能是食管鳞癌发生的早期事件之一,为食管鳞癌的早期筛选及诊断提供了一个潜在的生物学指标。
Objective:
To detect the CpG Islands which in promoter region methylation status of RIZ1 gene in the human esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue. And analyze their relationship with the clinical and pathological features of the human esophageal squamous carcinoma.
Methods:
The human esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue specimens and their clinical and pathological data were collected.
Methylation-specific PCR(MSP) was used to investigate the CpG Islands which in promoter region methylation status of RIZ1 gene in the carcinoma tissues, the adjacent tissues and the normal tissues from 47 esophageal squamous cell carcinoma patients. The relationship between the CpG Islands which in promoter region methylation status of RIZ1 gene and the clinical and pathological features of the patients was analyzed combined with the related data such as gender, age, family history, cell differentiation of tumor, TNM classification of tumor and clinical stage of the 47 esophageal squamous cell carcinoma patients.
Results:
The cases of CpG Islands which in promoter region methylation of RIZ1 gene were found 26,3,0 in the esophageal squamous carcinoma tissue, the adjacent tissue and the normal tissue specimens of 47 esophageal squamous cell carcinoma patients respectively. The positivity rate of promoter region methylation of RIZ1 gene was significantly higher in esophageal squamous carcinoma tissue than that in adjacent tissue and the normal tissue (55.3%vs.6.4%and 0.0%, P<0.01), but no significant difference was detected between the adjacent tissue and the normal tissue (P>0.05). We found that the CpG Islands which in promoter region methylation status of RIZ1 gene in the human esophageal squamous carcinoma had no obviously relationship with the data of gender, age, family history, cell differentiation of tumor, TNM classification of tumor and clinical stage (P>0.05, respectively)
Conclusion:
Promoter hypermethylation of RIZ1 gene is present in the human esophageal squamous carcinoma, suggesting that the promoter hypermethylation of RIZ1 gene was associated with the tumorigenesis of human esophageal squamous carcinoma, it may be a significant contributing factor to tumour development in human esophageal squamous carcinoma. The CpG Islands which in promoter region methylation of RIZ1 gene may be one of the earlist events in the occurrence of human esophageal squamous carcinoma, it may provide help in the early detection of esophageal squamous carcinoma.
引文
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