香蕉枯萎病菌4号小种鉴别培养基研制与应用
摘要
香蕉枯萎病威胁着我国香蕉产业发展。香蕉枯萎病菌的快速检测技术,有助于香蕉枯萎病的早期诊断和对病害实施有效监控。
2006～2008年,开展了香蕉枯萎病菌的快速检测技术研究,取得以下结果:
1.鉴定培养基的研制通过对KOMADA培养基和K2培养基的改良研究,研制了KM培养基。KM培养基是专用于香蕉枯萎病菌4号小种检测的鉴定培养基。香蕉枯萎病菌4号生理小种在KM培养基上形成的齿轮状菌落是重要的鉴别特征;KM培养基选择性强,能有效抑制杂菌和有利于香蕉枯萎病菌4号小种生长。
2.鉴别培养基的应用该鉴别培养基在生产上试用,从香蕉病组织和病田土壤中检测出具有明显鉴别特征的菌落,检出菌通过形态学鉴定、致病性测定、营养亲和性测定、ITS-PCR和RAPD-PCR检测,证实了KM检出的目标菌为香蕉枯萎病菌4号生理小种。应用试验证明了KM鉴别培养基可以用于检测香蕉病组织和田间土壤中的香蕉枯萎病菌4号小种,是一种快速、可靠、简便和实用的检测技术。
KM鉴别培养基检测技术与致病性测定、营养亲和性测定、ITS-PCR和RAPD-PCR检测等方法相结合,构建成香蕉枯萎病菌生理小种的检测技术体系。
Fusarium wilt of banana(Panama disease) is an important threat to the development of banana industry in China. A rapid detection technology of Fusarium oxysporum f.sp. cubense will contribute to early diagnosing and effective monitoring of the disease.
From 2006 to 2008, the rapid detection technology of Fusarium oxysporum f.sp. cubense was studied and achieved the main results as follows:
1 .Developed KM differential medium. KM medium was prepared by modifying the formulation of KOMADA and K2, It was a differential medium for detection of. Fusarium oxysporum f.sp. cubense race 4. The pathogenic fungus formed gear edge colony on KM medium which is an important diagnosis characteristics. KM differential medium is highly selectivity, effectively inhibited other microorganisms and promoted the growth of Fusarium oxysporum f.sp. cubense race 4.
2. Application of the differential medium. KM medium was tested in the field of fusarium wilt of banana disease, and the isolates were detected from diseased banana tissue and soil by gear edge colony. The isolates had been proved to be Fusarium oxysporum f.sp. cubense race 4 by morphological identification, pathogenicity tests, vegetative compatibility tests, DNA-ITS and RAPD-PCR analysis. KM differential medium was proved to qualify for identifying Fusarium oxysporum f.sp. cubense race 4 from infected tissue and soil in the application tests, the technique was a rapid, reliable, convenient and practical detection method.
The detection technology of KM differential medium will integrate with the pathogenicity tests, vegetative compatibility tests, DNA-ITS or RAPD-PCR analysis to establish a detection system of Fusarium oxysporum f.sp. cubense race 4.
2006～2008年,开展了香蕉枯萎病菌的快速检测技术研究,取得以下结果:
1.鉴定培养基的研制通过对KOMADA培养基和K2培养基的改良研究,研制了KM培养基。KM培养基是专用于香蕉枯萎病菌4号小种检测的鉴定培养基。香蕉枯萎病菌4号生理小种在KM培养基上形成的齿轮状菌落是重要的鉴别特征;KM培养基选择性强,能有效抑制杂菌和有利于香蕉枯萎病菌4号小种生长。
2.鉴别培养基的应用该鉴别培养基在生产上试用,从香蕉病组织和病田土壤中检测出具有明显鉴别特征的菌落,检出菌通过形态学鉴定、致病性测定、营养亲和性测定、ITS-PCR和RAPD-PCR检测,证实了KM检出的目标菌为香蕉枯萎病菌4号生理小种。应用试验证明了KM鉴别培养基可以用于检测香蕉病组织和田间土壤中的香蕉枯萎病菌4号小种,是一种快速、可靠、简便和实用的检测技术。
KM鉴别培养基检测技术与致病性测定、营养亲和性测定、ITS-PCR和RAPD-PCR检测等方法相结合,构建成香蕉枯萎病菌生理小种的检测技术体系。
Fusarium wilt of banana(Panama disease) is an important threat to the development of banana industry in China. A rapid detection technology of Fusarium oxysporum f.sp. cubense will contribute to early diagnosing and effective monitoring of the disease.
From 2006 to 2008, the rapid detection technology of Fusarium oxysporum f.sp. cubense was studied and achieved the main results as follows:
1 .Developed KM differential medium. KM medium was prepared by modifying the formulation of KOMADA and K2, It was a differential medium for detection of. Fusarium oxysporum f.sp. cubense race 4. The pathogenic fungus formed gear edge colony on KM medium which is an important diagnosis characteristics. KM differential medium is highly selectivity, effectively inhibited other microorganisms and promoted the growth of Fusarium oxysporum f.sp. cubense race 4.
2. Application of the differential medium. KM medium was tested in the field of fusarium wilt of banana disease, and the isolates were detected from diseased banana tissue and soil by gear edge colony. The isolates had been proved to be Fusarium oxysporum f.sp. cubense race 4 by morphological identification, pathogenicity tests, vegetative compatibility tests, DNA-ITS and RAPD-PCR analysis. KM differential medium was proved to qualify for identifying Fusarium oxysporum f.sp. cubense race 4 from infected tissue and soil in the application tests, the technique was a rapid, reliable, convenient and practical detection method.
The detection technology of KM differential medium will integrate with the pathogenicity tests, vegetative compatibility tests, DNA-ITS or RAPD-PCR analysis to establish a detection system of Fusarium oxysporum f.sp. cubense race 4.
引文
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