过氧化物酶体增殖物激活受体γ在急性胰炎中的表达及意义
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摘要
目的:观察过氧化物酶体增殖物激活受体(PPARγ)在急性胰腺炎(acutepancreatitis AP)中的表达及其与胰腺病理改变之间的关系,探讨PPARγ在AP机制中作用。
方法:54只wistar大鼠随机分为轻症胰腺炎组(M组)、重症胰腺炎组(S组)、对照组(C组)应法检测胰腺组三组,采用胰胆管注射方法复制胰腺炎动物模型。在各时间点以光镜观察胰腺病变,用免疫组化和反转录聚合酶链式反织中PPARγ蛋白及PPARγmRNA的表达。
结果:C组1、6、12小时各时间点PPARγ蛋白及PPARγmRNA的表达无统计学意义,M组、S组12小时点PPARγ蛋白及PPARγmRNA的表达高于1、6小时点,具有统计学意义(P＜0.05)。在12小时点S组PPARγ表达最高,其次为M组,表达最低的为C组,三组间具有统计学意义(P＜0.05)。胰腺的病理形态变化与PPARγ蛋白及PPARγmRNA的表达成正相关。
结论:PPARγ参与急性胰腺炎的发生发展。与病理类型关系密切,其水平可作为急性胰腺炎的预后指标
Objective:To observe the changes of PPARγin rats with acute pancreatitis,and to explore relationship between PPARγwith acute pancreatitis.
Methods:Adult Wistar rats were randomly divided into three groups.Eight rats of each groups were killed at 1,6,12h points after model was established and pancreatic tissue was collected.RT-PCR and immunohistochemistry were used to detect the lever of PPARγmRNA and protein in 54 pancreatic tissue of rats with acute pancreatitis.The relationship between the expression and the pathological changes was analyzed statistically.
Results:At 1 6 12hour the leves of PPARγand PPARγmRNA has no significantly in C group(p＜0.05).at 12 hour the leves of PPARγand PPARγmRNA in C Group and Mgroup is higher than Cgroups(p＜0.05);at 12 hour the expressions of PPARγand PPARγmRNA in Cgroups is highest,Cgroups is lowest(p＜0.05).The expression of PPARγand PPARγmRNA is positive correlated with the scores of pancreatic tissue pathology(r=0.341 0.311).
Conclusion:PPARγmay play an important role in the pathogenesis or progression of acute pancreatitis,and the level of PPARγmay be a prognostic indicator in actue pancreatitis.
方法:54只wistar大鼠随机分为轻症胰腺炎组(M组)、重症胰腺炎组(S组)、对照组(C组)应法检测胰腺组三组,采用胰胆管注射方法复制胰腺炎动物模型。在各时间点以光镜观察胰腺病变,用免疫组化和反转录聚合酶链式反织中PPARγ蛋白及PPARγmRNA的表达。
结果:C组1、6、12小时各时间点PPARγ蛋白及PPARγmRNA的表达无统计学意义,M组、S组12小时点PPARγ蛋白及PPARγmRNA的表达高于1、6小时点,具有统计学意义(P＜0.05)。在12小时点S组PPARγ表达最高,其次为M组,表达最低的为C组,三组间具有统计学意义(P＜0.05)。胰腺的病理形态变化与PPARγ蛋白及PPARγmRNA的表达成正相关。
结论:PPARγ参与急性胰腺炎的发生发展。与病理类型关系密切,其水平可作为急性胰腺炎的预后指标
Objective:To observe the changes of PPARγin rats with acute pancreatitis,and to explore relationship between PPARγwith acute pancreatitis.
Methods:Adult Wistar rats were randomly divided into three groups.Eight rats of each groups were killed at 1,6,12h points after model was established and pancreatic tissue was collected.RT-PCR and immunohistochemistry were used to detect the lever of PPARγmRNA and protein in 54 pancreatic tissue of rats with acute pancreatitis.The relationship between the expression and the pathological changes was analyzed statistically.
Results:At 1 6 12hour the leves of PPARγand PPARγmRNA has no significantly in C group(p＜0.05).at 12 hour the leves of PPARγand PPARγmRNA in C Group and Mgroup is higher than Cgroups(p＜0.05);at 12 hour the expressions of PPARγand PPARγmRNA in Cgroups is highest,Cgroups is lowest(p＜0.05).The expression of PPARγand PPARγmRNA is positive correlated with the scores of pancreatic tissue pathology(r=0.341 0.311).
Conclusion:PPARγmay play an important role in the pathogenesis or progression of acute pancreatitis,and the level of PPARγmay be a prognostic indicator in actue pancreatitis.
引文
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【5】 张永宏,周力,黄立敏等,细胞凋亡和肿瘤坏死因子在大鼠急性胰腺炎发病中的作用.贵州医药,2004,28:1081-1083.
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【7】 张在兴,孙家邦,李非等,内源性一氧化氮对大鼠急性坏死性胰腺炎的胰腺的保护作用.中华普通外科杂志,2000,(15):606-608.
【8】 赵敏,陈瑞芬,周以键等,内源性一氧化氮在急性出血坏死性胰腺炎发病中的作用.首都医科大学学报.2003,24:243-246.
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【2】 Knaus,W.A.,E.A.Draper,et al.(1985).APACHE II:a severity of disease classification system.Crit Care Med 13(10):818-29.
【3】 陈昆,梁坚,汤斌等,急性胰腺炎患者血清TNF-α、L-6和 IL-8含量的测定.上海免疫学杂志,2002,29:343-346.
【4】 常华,严级慎,闫俊玲,大鼠急性胰腺炎腺泡细胞凋亡与外周血浆TNF-γ的关系.胆胰外科杂志,2002,14:215-216.
【5】 张永宏,周力,黄立敏等,细胞凋亡和肿瘤坏死因子在大鼠急性胰腺炎发病中的作用.贵州医药,2004,28:1081-1083.
【6】 赵敏,陈瑞芬,周以键等,内源性一氧化氮在急性出血坏死性胰腺炎发病中的作甩.首都医科大学学报,2003,(24):243-246.
【7】 张在兴,孙家邦,李非等,内源性一氧化氮对大鼠急性坏死性胰腺炎的胰腺的保护作用.中华普通外科杂志,2000,(15):606-608.
【8】 赵敏,陈瑞芬,周以键等,内源性一氧化氮在急性出血坏死性胰腺炎发病中的作用.首都医科大学学报.2003,24:243-246.
【9】 KaiserAM,Saluja AK,Sengupta A,SalujaM,steer ML.Relationship between severity,necrosis,and apoptosis in five models of experimental acute pancreatitis.Am J physiol 1995269(5):1295-1304.
【10】 张延龄,外科严重感染中的抗生素和新颖治疗剂.国外医学分册,1997,24(2):65.
【11】 Tashiro M,Nakamura H,Taguchi M,et al.Expression of survivin after acute necrohemorrhagic pancreatitis in rats.Pancreas,2003,26(2):160-165.
【12】 Kinnala PJ,Kuttila KT,Gronroos JM,et al.Pancreatic tissue perfusion in experimental acute pancreatitis[J].Eur J Surg, 2001,167 9 :689 - 694.
[13] SunamuraM, Yamauchi J, Shibuya K, et al. Pancreatic microcir-culation in acute pancreatitis[J]. J Hepa Panc Surg, 1998, 5 (1 ) :62-68.
[14] DoboszM,Mionskowska L,Hac S, et al. Heparin improves organ microcirculatory disturbances in caerulein-induced acute pancreatitis in rats[J].World J Gastro,2004,10 (17) :2553 - 2556.
[15] Foitzik T Hotz HG Eibl G et al Therapy for microcirculatory disorders in severe acute pancreatitis effectiveness of platelet activatin factor receptor blockade V s Endothelin receptor blockade J Gastrointestinal Surg , 19993 (3): 244.
[16] Al-Bahrani, A. Z. and B. J. Ammori (2005). Clinical laboratory assessment of acute pancreatitis.Clin Chim Acta 362(1-2): 26-48.
[17] Gloor, B., C. A. Muller, et al. (2001). "Pancreatic infection in severe pancreatitis: the role of fungus and multiresistant organisms." Arch Surg 136(5): 592-596.
[18] Li Y ,Zhou ZG ,Zhang J,Chen YD,Li HG,Gao HK,Wang R, Microcirculatory detection of Toll-like receptor 4 in rat pancreas and intestine. Clin Hemorheol Microcirc 2006; 34: 213-219.
[19] Samel ST, Neufang T, Mueller A, Leister I, Becker H, Post S. A new abdominal cavity chamber to tudy the impact of increased intra-abdominal pres-sure on microcirculation of gut mucosa by using video microscopy in rats. Crit Care Med 2002; 30: 1854-1858.
[20] Van Felius ID, Akkermans LM, Bosscha K, Verheem A, Harmsen W, Visser MR, Gooszen HG Interdi-gestive small bowel motility and duodenal bacterial overgrowth in experimental acute pancreatitis. Neurogastroenterol Motil 2003; 15: 267-276.
[21] Rahman SH, Ibrahim K,Larvin M ,et al.Association of antioxidant enzyme gene polymorphisms and glutathione status with severe cute pancreatitis.Gastroenterology,2004,126 5:1312-1322.
【22】 裘莹,历曙光.急性胰腺炎大鼠钙-镁ATP酶的变化及维生素E 的影响.同济大学学报,2002,23:276-278。
【23】 YadavD,P itchumoniCS.Issues in hyperlip idem ic panereatitis[J].J Clin astroentero l,2003,36(1):54-62.
【24】 Jap TS,Jenq SF,W u YC,et al.Mutations in the lipop ro tein lipase gene as a cause of hypert glyce demia and pancreatitis in Taiwan[J].Pancreas,2003,27(2):122-126.
【25】 Yeh JH,Chen JH,Ch iu HC.P Iamapheresis fo r hyperlip idem ic pan-creatitis[J].J Clin Apheresis,2003,18(4):181-85.
【26】 Fortson MR.Freedman SN.Webster PD.Clinical assessment of hyperlipideamic pancreatitis.Am J Gastroenterol,1995,90:2134-2139.
【27】 Toskes PP.hyperlipidemic pancreatitis J.Gastroenterol Clin North Am,1990,19:783-791.
【28】 De Chalain TMB,Michell WL,Berger GMB.Hyperlipimia pregnancy and pancreatitis J.Surg Gynecol Obstet,1988,167:469-473.
【29】 Huang G,Yao J,Zeng W,et al.ER stress disrupts Ca2 + - signaling complexes ang Ca2+regulation in secretory and muscle cells from PEPK-knowout mice.J Cell Sci,2006,119:153-161.
【30】 Berger Z,Q uera R,Poniach ik J,et al.Heparin and insulin treatment of acute pancreatitis caused by hypertriglyeeridem ia.Experience of 5 cases[J].RevM ed Ch il,2001,129(12):1373-1378.
【31】 Meirhaeghe A,Fajas L,Gouilleux F,et al.A functiona polymorphism in a STAT5B site of the human PPAR gamma 3gene promoter affects height and lipid metabolism in a French population.Arterioscler Thromb Vasc Biol,2003,23:289-294.
【32】 Berger J,Moiler DE.The mechanisms of action of PPARSJ.Annu Rev Med,2002,53:409-435.
【33】 Willson TM,Brown PJ,Sternbach DD,et al.The PPAR from orphan receptors to drug discovery.J Med Chem,2000 43:527-550.
【34】 Jiang C,Ting AT,Seed B.PPAR-gamma agonists Inhibit production of monocyte inflammatory cytokines.Nature,1998391:82-86.
Ricote M,Li AC,Willson TM et al.The peroxisom proliferator-activated receptor-gamma is a negative regulator of macrophage activation.Nature,1998,391:79-82.
【35】 Aho H J,Koskensalo SM,Nevalainen TJ et al.Experimental pancreatitis in the rat:Sodium taurocholate2-nduced acute haemorrhagic pancreatitis.Scand J Gast roenterol,1980,15411-416.
【36】 陶坤,张义胜等,血栓素前列环素在大鼠轻症急性胰腺炎向重症急性胰腺炎转变中的变化和意义.肝胆外科杂志.2007,15(3):226-228.
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