金属蛋白酶组织抑制剂（多西环素）对大肠癌裸鼠移植瘤的影响研究
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摘要
目的观察多西环素对大肠癌裸鼠皮下移植瘤的影响,为大肠癌的治疗寻求一个新的可能治疗途径。
方法将10只BALB/C(nu/nu)裸鼠按随机数字表法均分为对照组和实验组,人大肠癌细胞系LoVo细胞种植入裸鼠皮下,建立裸鼠皮下人大肠癌移植瘤模型。对照组应用生理盐水瘤内多点直接注射,实验组应用多西环素瘤内多点直接注射治疗。然后记录肿瘤大小,观察肿瘤生长曲线,计算肿瘤生长抑制率。
结果实验表明实验组肿瘤较对照组肿瘤生长明显抑制,第18天起实验组和对照组肿瘤体积出现差别,对照组肿瘤和实验组肿瘤体积分别为(1.0691±0.2148)cm~3、(0.6337±0.1088)cm~3,第26天对照组肿瘤和实验组肿瘤体积分别为(1.7558±0.2038)cm~3、(0.8898±0.0808)cm~3。实验组肿瘤体积较对照组体积明显减小,生长抑瘤率为49.7%(F=78.041 p<0.01)。
结论多西环素可以抑制大肠癌裸鼠皮下移植瘤的的生长。有可能应用于人大肠癌的治疗。
Objective To investigate the inhibitory effects of Doxycycline on the growth of human colorectal cancer implanted in nude mice.So that we can look for a new therapy for human colorectal cancer.
Methods Ten BALB/C nude mice were randomly divided into control group and study group equally.Human colorectal cancer cell line LoVo was inoculated subcutaneously into nude mice to form transplantation tumors.Saline was injected to mice in control group and Doxycycline was injected to mice in study group.The changes of tumors in both groups were recorded and the curve of tumor growth was also observed and the inhibitory rate of tumor was calculated.
Results Doxycycline significantly inhibited the growth of colorectal cancer xenograf- ts in nude mice. The first 18 days from the experimental group and control group differences in tumor size(1.0691±0.2148)cm3、(0.6337±0.1088)cm3, the first 26 days of tumor control group and experimental group were tumor size (1.7558±0.2038) cm3, (0.8898±0.0808) cm3.The volume of transplantation tumor reduced with the inhibitory rate of 49.7%,which showed that the former was significantly lower than that of the control group (F=78.041 p<0.01).
Conclusion Doxycycline may inhibit the growth of colorectal cancer bearing nude mice.Which maybe uses for the therapy of human colorectal cancer.
方法将10只BALB/C(nu/nu)裸鼠按随机数字表法均分为对照组和实验组,人大肠癌细胞系LoVo细胞种植入裸鼠皮下,建立裸鼠皮下人大肠癌移植瘤模型。对照组应用生理盐水瘤内多点直接注射,实验组应用多西环素瘤内多点直接注射治疗。然后记录肿瘤大小,观察肿瘤生长曲线,计算肿瘤生长抑制率。
结果实验表明实验组肿瘤较对照组肿瘤生长明显抑制,第18天起实验组和对照组肿瘤体积出现差别,对照组肿瘤和实验组肿瘤体积分别为(1.0691±0.2148)cm~3、(0.6337±0.1088)cm~3,第26天对照组肿瘤和实验组肿瘤体积分别为(1.7558±0.2038)cm~3、(0.8898±0.0808)cm~3。实验组肿瘤体积较对照组体积明显减小,生长抑瘤率为49.7%(F=78.041 p<0.01)。
结论多西环素可以抑制大肠癌裸鼠皮下移植瘤的的生长。有可能应用于人大肠癌的治疗。
Objective To investigate the inhibitory effects of Doxycycline on the growth of human colorectal cancer implanted in nude mice.So that we can look for a new therapy for human colorectal cancer.
Methods Ten BALB/C nude mice were randomly divided into control group and study group equally.Human colorectal cancer cell line LoVo was inoculated subcutaneously into nude mice to form transplantation tumors.Saline was injected to mice in control group and Doxycycline was injected to mice in study group.The changes of tumors in both groups were recorded and the curve of tumor growth was also observed and the inhibitory rate of tumor was calculated.
Results Doxycycline significantly inhibited the growth of colorectal cancer xenograf- ts in nude mice. The first 18 days from the experimental group and control group differences in tumor size(1.0691±0.2148)cm3、(0.6337±0.1088)cm3, the first 26 days of tumor control group and experimental group were tumor size (1.7558±0.2038) cm3, (0.8898±0.0808) cm3.The volume of transplantation tumor reduced with the inhibitory rate of 49.7%,which showed that the former was significantly lower than that of the control group (F=78.041 p<0.01).
Conclusion Doxycycline may inhibit the growth of colorectal cancer bearing nude mice.Which maybe uses for the therapy of human colorectal cancer.
引文
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