2008-2009年兰州地区儿童急性呼吸道感染病毒病原学研究
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摘要
实验目的
了解兰州地区急性呼吸道感染(ARTIs)患儿中呼吸道病原谱的分布现状,为本地区儿童急性呼吸道感染的防治提供基础数据。明确新发现病毒人博卡2病毒在呼吸道中的致病性。
对象及方法
1收集兰州大学第一医院儿科2008年11月到2009年10月因急性呼吸道感染就诊患儿的鼻咽抽吸物(Nasopharyngea aspirates, NPAs)和门诊患儿的咽拭子(throat swabs)及正常体检儿童的呼吸道分泌物标本。
2分别采用聚合酶链反应(PCR)检测呼吸道合胞病毒(RSV)、流感病毒(IFVA、B型)、和副流感病毒(PIV 1-3型)、鼻病毒(HRV)、人偏肺病毒(HMPV)、冠状病毒-NL63(HCOV-NL63)、冠状病毒-HKU1(HCOV-HKU1)、冠状病毒-229E(HCOV-229E)、冠状病毒-OC43(HCOV-OC43)、多瘤病毒WU (WUPyV)和多瘤病毒KI (KIPyV) 14种常见及新发现呼吸道感染相关的病毒。结合详细的临床资料,做出临床流行病学分析。
3建立博卡病毒的病例对照研究：采用巢式PCR (Nest-pcr)方法检测急性呼吸道感染患儿和正常儿童呼吸道标本中的HBoV-1和HBoV-2基因,并对扩增产物测序及进行Blast基因序列比对。讨论其在呼吸道中的致病性。
结果
1.在收集的510份呼吸道标本中,共检出病毒感染阳性病例382例(检出率74.9%)。其中RSV阳性扩增产物160份,检出率为31.4%；IFVA阳性扩增产物53份,检出率为10.4%; IFVB阳性扩增产物25份,检出率为4.9%；PIV3阳性扩增产物83份,检出率为16.1%；HRV阳性扩增产物48份,检出率为9.4%；HMPV阳性扩增产物16份,检出率为0.4%；HCoV-NL63阳性扩增产物3份,检出率为0.6%；HCoV-HKU1阳性扩增产物2份,检出率为0.4%; WUPyV阳性扩增产物7份,检出率为1.4%,KIPyV阳性扩增产物15份,检出率为2.9%,未检出PIV-1/-2和HCoV-229E/OC43。314例男性患者中病毒感染216例(58.3%),女性196例感染166例(55.5%),男女感染率的之比为1.3：1；患儿年龄最小10天,最大14岁,中位年龄28个月。其中1岁以下患儿病毒感染208例,占总病毒感染人数的54.5%；1～3岁患儿病毒感染84例,占22%；3～5岁患儿病毒感染54例,占14.1%；大于5岁患儿病毒感染36例,占9.4%。随着年龄的增长,病毒阳性检出率逐渐下降。本地区儿童病毒性急性呼吸道感染的季节分布较明显,以冬春季节为主。
510份病例组呼吸道患儿标本检测到7例HBoV-2阳性扩增产物,检出率为1.4%。将阳性PCR产物进行序列测定及分析,发现其与GeneBank中已知标准株(Human bocavirus 2 strain HBoV2B-NI-213)序列的同源性在98%-100%之间,提示HBoV-2兰州株的核苷酸变异较小。患儿感染年龄从4个月到65个月,中位年龄8.5个月,除一例标本外,其余均在2岁以下儿童。全部为男性患儿。检出病例主要在冬春季节,12月检出最多。该病毒混合感染率高达57.1%,与RSV混合感染最多。临床诊断包括喘息性支气管肺炎、急性支气管肺炎、急性支气管炎,其中喘息性支气管肺炎最多见,为5例。HBOV-2在对照儿童中检出率为1.4%(4/290),HBOV-2阳性的患儿均≤6个月,标本分别在3月,5月,6月,8月中检出,男女比例为3：1。病例组中HBOV-1在病例组中检出率为14.3%(73/510),正常儿童中HBOV-1的检出率为3.1%(9/290)。
结论
1本研究中74.9%的患儿病毒检测阳性,结合本课题组2006-2008年的研究结果说明,病毒病原在兰州地区儿童呼吸道感染中仍占有重要地位。在2008-2009年的研究中发现儿童病毒性呼吸道感染病原以RSV、PIV-3、HRV及IFVA占主要地位,与2007-2008年相比IFVA及PIV-3两种呼吸道病毒在本地区的感染有所上升,跟2007-2008的呼吸道病毒感染谱略有差异,呼吸道病毒病原分布具有明显的季节分布特征,以冬春季节多见。
2 HBOV-2与疾病：P>0.005 (Fisher's Exact Test)无统计学意义。HBVO-2感染与呼吸道疾病无关。HBOV-1与疾病：p<0.005 (Pearson Chi-Square)有统计学意义,病例组中感染HBOV-1更高。HBOV-1可以引起呼吸道疾病。
Objective
In this study, in order to provide basic data and scientific evidence for the prevention and control of acute respiratory tract infection (ARTI), we investigated the common and potential viral etiological agent of ARTI among children in Lanzhou area. Meanwhile, we identified the pathogenesis of HBoV-2 in the respiratory tract infection.
Subjects and Methods
510 Nasopharyngea aspirates (NPAs) and throat swabs specimens were collected from children with acute respiratory infections and 290 specimes from healthy children from November 2008 to October 2009 in Lanzhou area of china.
RSV, Influenza A and B, Parainflurnzal-3, HRV, HMPV, HCoV-NL63, HCoV-HKU1, HCoV-229E, HCoV-NL63, WUPyV and KIPyV were detected by RT-PCR and nest-PCR methods, and amplicons were identified by sequencing. Clinical and epidemiological analysis on acute respiratory tract infections among children which cause by diverse viruses were performed.
The establishment of a case-control investigation:HBoV-1 and HBoV-2 were also tested by nested-PCR methods. The amplicons were identified by sequencing and the sequences were then used to search against those in the NCBI databases using Blast program. Meanwhile, The relatedness of HBoV-2 and newly identified Bocavirus to respiratory tract infection was also investigated in this study.
Results
1.382 samples were detected positive in 510 NPAs samples which were collected in 2008-2009 year and the total rate was 74.9%. Respectively, the rate of RSV was 31.4% (160/510), IFVA was 10.4%(53/510), IFVB was 4.9%(625/510), PIV3 was 16.1%(83/510), HRV was 9.4%(48/510), HMPV was 0.4%(16/510), HCoV-NL63 was 0.6%(3/510), HCoV-HKUl was 0.4%(2/510), WUPyV was 1.4%(7/510), KIPyV was 2.9%(15/510). PIV-1/-2 and HCoV-229E/OC43 were not detected. Among the 510 NPAs samples, the positive rate of samples collected from male patients was 58.3%(216/314) while that of samples from female cases was 55.5%(166/196). The ratio of male to female patients was 1.3:1. The median ages of the viruses infected patients was 28 months (range from 10 days to 14 years old).54.5%(208/510) children with viruses infection were under 1 year of age,18.1%(84/510) children were at age from 1 to 3 years,14.1%(54/510) children were at age from 3 to 5 years and 9.4%(36/510) children were older than 5 years old. The positive rate of virus detection decreased in accordance with the increasing of age. The detected rate had no significant difference among the age groups (P=0.989>0.05). There were remarkable seasonal distributions of viral respiratory tract infections in Lanzhou area, viral respiratory tract infections predominated in spring and winter.
2. The detection rate of HBoV-2 was 1.4%(7/510). The positive amplicons were sequenced and then analyzed by using Blast program. The results showed that the sequences shared 98%～100% homology with Human bocavirus 2 strain HBOV2B-NI-213 in the NCBI nucleotide database except single insertions and mutations. It indicated that there was few diversity in the HBoV-2 Lanzhou isolates. The median ages of the viruses infected children was 8.5 months (range from 4 months to 65 months). All infected children except one were under 2 years old and interestingly all of them were male. The detected cases of viral respiratory tract infections were mainly during spring and winter, especially in December (3 cases). The cases of HBoV-2 usually coinfected with other viruses, mostly with RSV. Clinical symptoms included asthmatic bronchial pneumonia, acute bronchial pneumonia and acute bronchitis, especially asthmatic bronchial pneumonia (5 cases). The detection rate of HBOV-2 among the control children was 1.4%(4/290). The infected children of HBOV-2 were=6 months old. The samples detected positive were in March, May, June and August and the ratio of male to female were 3:1. The detection rate of HBOV-1 among the infected children was 14.3%(73/510) while it was 3.2% of healthy children.
Conclusions
Viral infection is the main reason of ALRTI of children in Lanzhou area and 74.9% of infected children were detected positive. RSV, PIV-3, HRV and IFVA are major causes of respiratory tract infections among children in this area. But there was a slightly different spectrum of respiratory tract infection representing the increasing of IFVA and PIV-3 infections comparing with that 2007-2008 year, and obvious seasonal distribution, especially in winter and spring.
The relatedness of HBoV2 to respiratory tract infection:there was no significance in statistics (P>0.005, Fisher's Exact Test). It indicated that HBoV-2 has no relationship with respiratory tract infection. Whereas, The HBoV-1 is related to respiratory tract infection according to analysis in statistics (p<0.005, Pearson Chi-Square). The detection rate of infected children was higher than that of control cases. And HBoV-1 can cause respiratory tract infection.
了解兰州地区急性呼吸道感染(ARTIs)患儿中呼吸道病原谱的分布现状,为本地区儿童急性呼吸道感染的防治提供基础数据。明确新发现病毒人博卡2病毒在呼吸道中的致病性。
对象及方法
1收集兰州大学第一医院儿科2008年11月到2009年10月因急性呼吸道感染就诊患儿的鼻咽抽吸物(Nasopharyngea aspirates, NPAs)和门诊患儿的咽拭子(throat swabs)及正常体检儿童的呼吸道分泌物标本。
2分别采用聚合酶链反应(PCR)检测呼吸道合胞病毒(RSV)、流感病毒(IFVA、B型)、和副流感病毒(PIV 1-3型)、鼻病毒(HRV)、人偏肺病毒(HMPV)、冠状病毒-NL63(HCOV-NL63)、冠状病毒-HKU1(HCOV-HKU1)、冠状病毒-229E(HCOV-229E)、冠状病毒-OC43(HCOV-OC43)、多瘤病毒WU (WUPyV)和多瘤病毒KI (KIPyV) 14种常见及新发现呼吸道感染相关的病毒。结合详细的临床资料,做出临床流行病学分析。
3建立博卡病毒的病例对照研究：采用巢式PCR (Nest-pcr)方法检测急性呼吸道感染患儿和正常儿童呼吸道标本中的HBoV-1和HBoV-2基因,并对扩增产物测序及进行Blast基因序列比对。讨论其在呼吸道中的致病性。
结果
1.在收集的510份呼吸道标本中,共检出病毒感染阳性病例382例(检出率74.9%)。其中RSV阳性扩增产物160份,检出率为31.4%；IFVA阳性扩增产物53份,检出率为10.4%; IFVB阳性扩增产物25份,检出率为4.9%；PIV3阳性扩增产物83份,检出率为16.1%；HRV阳性扩增产物48份,检出率为9.4%；HMPV阳性扩增产物16份,检出率为0.4%；HCoV-NL63阳性扩增产物3份,检出率为0.6%；HCoV-HKU1阳性扩增产物2份,检出率为0.4%; WUPyV阳性扩增产物7份,检出率为1.4%,KIPyV阳性扩增产物15份,检出率为2.9%,未检出PIV-1/-2和HCoV-229E/OC43。314例男性患者中病毒感染216例(58.3%),女性196例感染166例(55.5%),男女感染率的之比为1.3：1；患儿年龄最小10天,最大14岁,中位年龄28个月。其中1岁以下患儿病毒感染208例,占总病毒感染人数的54.5%；1～3岁患儿病毒感染84例,占22%；3～5岁患儿病毒感染54例,占14.1%；大于5岁患儿病毒感染36例,占9.4%。随着年龄的增长,病毒阳性检出率逐渐下降。本地区儿童病毒性急性呼吸道感染的季节分布较明显,以冬春季节为主。
510份病例组呼吸道患儿标本检测到7例HBoV-2阳性扩增产物,检出率为1.4%。将阳性PCR产物进行序列测定及分析,发现其与GeneBank中已知标准株(Human bocavirus 2 strain HBoV2B-NI-213)序列的同源性在98%-100%之间,提示HBoV-2兰州株的核苷酸变异较小。患儿感染年龄从4个月到65个月,中位年龄8.5个月,除一例标本外,其余均在2岁以下儿童。全部为男性患儿。检出病例主要在冬春季节,12月检出最多。该病毒混合感染率高达57.1%,与RSV混合感染最多。临床诊断包括喘息性支气管肺炎、急性支气管肺炎、急性支气管炎,其中喘息性支气管肺炎最多见,为5例。HBOV-2在对照儿童中检出率为1.4%(4/290),HBOV-2阳性的患儿均≤6个月,标本分别在3月,5月,6月,8月中检出,男女比例为3：1。病例组中HBOV-1在病例组中检出率为14.3%(73/510),正常儿童中HBOV-1的检出率为3.1%(9/290)。
结论
1本研究中74.9%的患儿病毒检测阳性,结合本课题组2006-2008年的研究结果说明,病毒病原在兰州地区儿童呼吸道感染中仍占有重要地位。在2008-2009年的研究中发现儿童病毒性呼吸道感染病原以RSV、PIV-3、HRV及IFVA占主要地位,与2007-2008年相比IFVA及PIV-3两种呼吸道病毒在本地区的感染有所上升,跟2007-2008的呼吸道病毒感染谱略有差异,呼吸道病毒病原分布具有明显的季节分布特征,以冬春季节多见。
2 HBOV-2与疾病：P>0.005 (Fisher's Exact Test)无统计学意义。HBVO-2感染与呼吸道疾病无关。HBOV-1与疾病：p<0.005 (Pearson Chi-Square)有统计学意义,病例组中感染HBOV-1更高。HBOV-1可以引起呼吸道疾病。
Objective
In this study, in order to provide basic data and scientific evidence for the prevention and control of acute respiratory tract infection (ARTI), we investigated the common and potential viral etiological agent of ARTI among children in Lanzhou area. Meanwhile, we identified the pathogenesis of HBoV-2 in the respiratory tract infection.
Subjects and Methods
510 Nasopharyngea aspirates (NPAs) and throat swabs specimens were collected from children with acute respiratory infections and 290 specimes from healthy children from November 2008 to October 2009 in Lanzhou area of china.
RSV, Influenza A and B, Parainflurnzal-3, HRV, HMPV, HCoV-NL63, HCoV-HKU1, HCoV-229E, HCoV-NL63, WUPyV and KIPyV were detected by RT-PCR and nest-PCR methods, and amplicons were identified by sequencing. Clinical and epidemiological analysis on acute respiratory tract infections among children which cause by diverse viruses were performed.
The establishment of a case-control investigation:HBoV-1 and HBoV-2 were also tested by nested-PCR methods. The amplicons were identified by sequencing and the sequences were then used to search against those in the NCBI databases using Blast program. Meanwhile, The relatedness of HBoV-2 and newly identified Bocavirus to respiratory tract infection was also investigated in this study.
Results
1.382 samples were detected positive in 510 NPAs samples which were collected in 2008-2009 year and the total rate was 74.9%. Respectively, the rate of RSV was 31.4% (160/510), IFVA was 10.4%(53/510), IFVB was 4.9%(625/510), PIV3 was 16.1%(83/510), HRV was 9.4%(48/510), HMPV was 0.4%(16/510), HCoV-NL63 was 0.6%(3/510), HCoV-HKUl was 0.4%(2/510), WUPyV was 1.4%(7/510), KIPyV was 2.9%(15/510). PIV-1/-2 and HCoV-229E/OC43 were not detected. Among the 510 NPAs samples, the positive rate of samples collected from male patients was 58.3%(216/314) while that of samples from female cases was 55.5%(166/196). The ratio of male to female patients was 1.3:1. The median ages of the viruses infected patients was 28 months (range from 10 days to 14 years old).54.5%(208/510) children with viruses infection were under 1 year of age,18.1%(84/510) children were at age from 1 to 3 years,14.1%(54/510) children were at age from 3 to 5 years and 9.4%(36/510) children were older than 5 years old. The positive rate of virus detection decreased in accordance with the increasing of age. The detected rate had no significant difference among the age groups (P=0.989>0.05). There were remarkable seasonal distributions of viral respiratory tract infections in Lanzhou area, viral respiratory tract infections predominated in spring and winter.
2. The detection rate of HBoV-2 was 1.4%(7/510). The positive amplicons were sequenced and then analyzed by using Blast program. The results showed that the sequences shared 98%～100% homology with Human bocavirus 2 strain HBOV2B-NI-213 in the NCBI nucleotide database except single insertions and mutations. It indicated that there was few diversity in the HBoV-2 Lanzhou isolates. The median ages of the viruses infected children was 8.5 months (range from 4 months to 65 months). All infected children except one were under 2 years old and interestingly all of them were male. The detected cases of viral respiratory tract infections were mainly during spring and winter, especially in December (3 cases). The cases of HBoV-2 usually coinfected with other viruses, mostly with RSV. Clinical symptoms included asthmatic bronchial pneumonia, acute bronchial pneumonia and acute bronchitis, especially asthmatic bronchial pneumonia (5 cases). The detection rate of HBOV-2 among the control children was 1.4%(4/290). The infected children of HBOV-2 were=6 months old. The samples detected positive were in March, May, June and August and the ratio of male to female were 3:1. The detection rate of HBOV-1 among the infected children was 14.3%(73/510) while it was 3.2% of healthy children.
Conclusions
Viral infection is the main reason of ALRTI of children in Lanzhou area and 74.9% of infected children were detected positive. RSV, PIV-3, HRV and IFVA are major causes of respiratory tract infections among children in this area. But there was a slightly different spectrum of respiratory tract infection representing the increasing of IFVA and PIV-3 infections comparing with that 2007-2008 year, and obvious seasonal distribution, especially in winter and spring.
The relatedness of HBoV2 to respiratory tract infection:there was no significance in statistics (P>0.005, Fisher's Exact Test). It indicated that HBoV-2 has no relationship with respiratory tract infection. Whereas, The HBoV-1 is related to respiratory tract infection according to analysis in statistics (p<0.005, Pearson Chi-Square). The detection rate of infected children was higher than that of control cases. And HBoV-1 can cause respiratory tract infection.
引文
[1]. 侯云德,急性呼吸道感染的病原学防治.中国协和医科大学出版社,2005-06-07.
[2]. D'Angio, C.T., High altitude as an explanation for bronchiolitis-associated hospitalizations. Pediatr Infect Dis J,2000.19(5):p.492.
[3]. Lowther, S.A., et al., Bronchiolitis-associated hospitalizations among American Indian and Alaska Native children. Pediatr Infect Dis J,2000.19(1):p.11-7.
[4]. Shay, D.K., et al., Bronchiolitis-associated hospitalizations among US children,1980-1996. JAMA,1999.282(15):p.1440-6.
[5]. Stang, P., N. Brandenburg, and B. Carter, The economic burden of respiratory syncytial virus-associated bronchiolitis hospitalizations. Arch Pediatr Adolesc Med,2001.155(1):p. 95-6.
[6]. Medici, M.C., et al., Four year incidence of respiratory syncytial virus infection in infants and young children referred to emergency departments for lower respiratory tract diseases in Italy: the "Osservatorio VRS" Study (2000-2004). New Microbiol,2006.29(1):p.35-43.
[7]. Ventre, K. and A. Randolph, Ribavirin for respiratory syncytial virus infection of the lower respiratory tract in infants and young children. Cochrane Database Syst Rev,2004(4):p. CD000181.
[8]. van Woensel, J.B., W.M. van Aalderen, and J.L. Kimpen, Viral lower respiratory tract infection in infants and young children. BMJ,2003.327(7405):p.36-40.
[9]. van den Hoogen, B.G., et al., A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med,2001.7(6):p.719-24.
[10]. Bern, R.A., et al., Granzyme A- and B-cluster deficiency delays acute lung injury in pneumovirus-infected mice. J Immunol,2010.184(2):p.931-8.
[11]. Dyer, K.D., et al., Pneumoviruses infect eosinophils and elicit MyD88-dependent release of chemoattractant cytokines and interleukin-6. Blood,2009.114(13):p.2649-56.
[12]. Percopo, C.M., et al., Pulmonary eosinophils and their role in immunopathologic responses to formalin-inactivated pneumonia virus of mice. J Immunol,2009.183(1):p.604-12.
[13]. Tecu, C., et al., First detection of human metapneumovirus in children with respiratory infections in Romania. Roum Arch Microbiol Immunol,2007.66(1-2):p.37-40.
[14]. Broor, S. and P. Bharaj, Avian and human metapneumovirus. Ann N Y Acad Sci,2007.1102: p.66-85.
[15]. Kikuta, H., [Human metapneumovirus]. Uirusu,2006.56(2):p.173-81.
[16]. Farnsworth, J.E., et al., Development of a method for bacteria and virus recovery from heating, ventilation, and air conditioning (HVAC) filters. J Environ Monit,2006.8(10):p.1006-13.
[17]. van den Hoogen, B.G., et al., Antigenic and genetic variability of human metapneumoviruses. Emerg Infect Dis,2004.10(4):p.658-66.
[18]. Kapp, C., WHO lowers figures on SARS infections. Lancet,2003.362(9391):p.1207.
[19]. Lai, R.Q., et al., [Pathological and ultramicrostructural changes of tissues in a patient with severe acute respiratory syndrome]. Zhonghua Bing Li Xue Za Zhi,2003.32(3):p.205-8.
[20]. Lai, M.D., Y.M. Zhu, and X.M. Gu, [Severe acute respiratory syndrome]. Zhejiang Da Xue Xue Bao Yi Xue Ban,2003.32(3):p.167-70.
[21]. Kissoon, N., Severe acute respiratory syndrome:providing care while minimizing personal risks. Indian Pediatr,2003.40(7):p.645-51.
[22]. Ding, Y.Q., et al., [Study on etiology and pathology of severe acute respiratory syndrome]. Zhonghua Bing Li Xue Za Zhi,2003.32(3):p.195-200.
[23]. van der Hoek, L., et al., Identification of a new human coronavirus. Nat Med,2004.10(4):p. 368-73.
[24]. Kahn, J.S. and K. McIntosh, History and recent advances in coronavirus discovery. Pediatr Infect Dis J,2005.24(11 Suppl):p. S223-7, discussion S226.
[25]. Woo, P.C., et al., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J Virol,2005.79(2):p.884-95.
[26]. Respiratory syncytial virus activity--United States,2003-2004. MMWR Morb Mortal Wkly Rep,2004.53(49):p.1159-60.
[27]. Goldrick, B.A., Emerging infections:respiratory syncytial virus. Am J Nurs,2004.104(11):p. 54-6.
[28]. Valarcher, J.F. and G. Taylor, Bovine respiratory syncytial virus infection. Vet Res,2007. 38(2):p.153-80.
[29]. Rosenberg, H.F., et al., The pneumonia virus of mice infection model for severe respiratory syncytial virus infection:identifying novel targets for therapeutic intervention. Pharmacol Ther,2005.105(1):p.1-6.
[30]. Kho, N., et al., Respiratory syncytial virus infection and neurologic abnormalities: retrospective cohort study. J Child Neurol,2004.19(11):p.859-64.
[31]. Walls, T., et al., Polymerase chain reaction in the management of disseminated adenovirus infection after stem cell transplantation. Pediatr Infect Dis J,2004.23(12):p.1167-9.
[32]. McConnell, M.J. and M.J. Imperiale, Biology of adenovirus and its use as a vector for gene therapy. Hum Gene Ther,2004.15(11):p.1022-33.
[33]. Adenovirus. Am J Transplant,2004.4 Suppl 10:p.101-4.
[34]. Li, L., et al., Endostatin gene therapy for liver cancer by a recombinant adenovirus delivery. World J Gastroenterol,2004.10(13):p.1867-71.
[35]. Fleury, S., et al., Helper-dependent adenovirus vectors devoid of all viral genes cause less myocardial inflammation compared with first-generation adenovirus vectors. Basic Res Cardiol,2004.99(4):p.247-56.
[36]. Cuesta, R., Q. Xi, and R.J. Schneider, Structural basis for competitive inhibition of eIF4G-Mnkl interaction by the adenovirus 100-kilodalton protein. J Virol,2004.78(14):p. 7707-16.
[37]. Ostlund, M.R., et al., Respiratory virus infections in Stockholm during seven seasons:a retrospective study of laboratory diagnosis. Scand J Infect Dis,2004.36(6-7):p.460-5.
[38]. Monto, A.S., Occurrence of respiratory virus:time, place and person. Pediatr Infect Dis J, 2004.23(1 Suppl):p. S58-64.
[39]. Ngama, M.J., et al., Comparison of three methods of collecting nasal specimens for respiratory virus analysis. East Afr Med J,2004.81(6):p.313-7.
[40]. Dunn, J.J., et al., Sensitivity of respiratory virus culture when screening with R-mix fresh cells. J Clin Microbiol,2004.42(1):p.79-82.
[41]. Abadesso, C., et al., Use of palivizumab to control an outbreak of syncytial respiratory virus in a neonatal intensive care unit. J Hosp Infect,2004.58(1):p.38-41.
[42]. Riedel, F., et al., Role of sensory neuropeptides in PIV-3-infection-induced airway hyperresponsiveness in guinea pigs. Respiration,1997.64(3):p.211-9.
[43]. Karron, R.A., et al., A live human parainfluenza type 3 virus vaccine is attenuated and immunogenic in healthy infants and children. J Infect Dis,1995.172(6):p.1445-50.
[44]. Karron, R.A., et al., Rapid detection of parainfluenza virus type 3 RNA in respiratory specimens:use of reverse transcription-PCR-enzyme immunoassay. J Clin Microbiol,1994. 32(2):p.484-8.
[45]. Falsey, A.R., et al., Long-term care facilities:a cornucopia of viral pathogens. J Am Geriatr Soc,2008.56(7):p.1281-5.
[46]. Hagglund, S., et al., A six-year study on respiratory viral infections in a bull testing facility. Vet J,2007.173(3):p.585-93.
[47]. Ding, Y., et al., Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in SARS patients:implications for pathogenesis and virus transmission pathways. J Pathol,2004.203(2):p.622-30.
[48]. Wu, X.D., et al., The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells. Cell Res,2004.14(5):p.400-6.
[49]. Midulla, F., et al., Respiratory syncytial virus, human bocavirus and rhinovirus bronchiolitis in infants. Arch Dis Child,2010.95(1):p.35-41.
[50]. Bacha, U., et al., Development of broad-spectrum halomethyl ketone inhibitors against coronavirus main protease 3CL(pro). Chem Biol Drug Des,2008.72(1):p.34-49.
[51]. Gagneur, A., et al., Materno-fetal transmission of human coronaviruses:a prospective pilot study. Eur J Clin Microbiol Infect Dis,2008.27(9):p.863-6.
[52]. Rowley, A.H. and S.T. Shulman, New developments in the search for the etiologic agent of Kawasaki disease. Curr Opin Pediatr,2007.19(1):p.71-4.
[53]. Bastien, N., et al., Human coronavirus NL-63 infections in children:a 1-year study. J Clin Microbiol,2005.43(9):p.4567-73.
[54]. Woo, P.C., et al., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia. J Infect Dis,2005.192(11):p.1898-907.
[55]. Woo, P.C., et al., Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia. Arch Virol,2005.150(11):p.2299-311.
[56]. Woo, P.C., et al., In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease. Microbiol Immunol,2005. 49(10):p.899-908.
[57]. Esper, F., Z. Ou, and Y.T. Huang, Human coronaviruses are uncommon in patients with gastrointestinal illness. J Clin Virol,2010.
[58]. Leung, T.F., et al., Epidemiology and clinical presentations of human coronavirus NL63 infections in hong kong children. J Clin Microbiol,2009.47(11):p.3486-92.
[59]. Chan, C.M., et al., Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus. J Clin Virol,2009.45(1):p.54-60.
[60]. Vijgen, L., et al., A pancoronavirus RT-PCR assay for detection of all known coronaviruses. Methods Mol Biol,2008.454:p.3-12.
[61]. Chung, J.Y., et al., Detection of viruses identified recently in children with acute wheezing. J Med Virol,2007.79(8):p.1238-43.
[62]. Pyrc, K., B. Berkhout, and L. van der Hoek, Identification of new human coronaviruses. Expert Rev Anti Infect Their,2007.5(2):p.245-53.
[63]. Pyrc, K., B. Berkhout, and L. van der Hoek, Antiviral strategies against human coronaviruses. Infect Disord Drug Targets,2007.7(1):p.59-66.
[64]. Osborn, J.E., et al., Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments. J Virol, 1974.13(3):p.614-22.
[65]. Galateau-Salle, F., et al., Detection of SV40-like DNA sequences in pleural mesothelioma, bronchopulmonary carcinoma and other pulmonary diseases. Dev Biol Stand,1998.94:p. 147-52.
[66]. Dorries, K., New aspects in the pathogenesis of polyomavirus-induced disease. Adv Virus Res, 1997.48:p.205-61.
[67]. Vago, L., et al., JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol,1996.12(2):p.139-46.
[68]. Miyamura, T., K. Yoshiike, and K.K. Takemoto, High-titer SV40 replication in human fibroblast cell lines derived from patients with Wiskott-Aldrich syndrome. Virology,1983. 129(2):p.479-83.
[69]. Hogan, T.F., et al., Survey of human polyomavirus (JCV, BKV) infections in 139 patients with lung cancer, breast cancer, melanoma, or lymphoma. Prog Clin Biol Res,1983.105:p. 311-24.
[70]. Hogan, T.F., et al., Human polyomavirus infections with JC virus and BK virus in renal transplant patients. Ann Intern Med,1980.92(3):p.373-8.
[71]. Hogan, T.F., et al., Rapid detection and identification of JC virus and BK virus in human urine by using immunofluorescence microscopy. J Clin Microbiol,1980.11(2):p.178-83.
[72]. Shah, K.V., et al., Investigation of human urogenital tract tumors of papovavirus etiology: brief communication. J Natl Cancer Inst,1978.60(3):p.579-82.
[73]. Endo, S., et al., JC virus agnoprotein colocalizes with tubulin. J Neurovirol,2003.9 Suppl 1: p.10-4.
[74]. Knoll, A., et al., Low frequency of human polyomavirus BKV and JCV DNA in urothelial carcinomas of the renal pelvis and renal cell carcinomas. Oncol Rep,2003.10(2):p.487-91.
[75]. Li, R.M., et al., BK virus and SV40 co-infection in polyomavirus nephropathy. Transplantation, 2002.74(11):p.1497-504.
[76]. Zambrano, A., et al., Detection of human polyomaviruses and papillomaviruses in prostatic tissue reveals the prostate as a habitat for multiple viral infections. Prostate,2002.53(4):p. 263-76.
[77]. Safdar, A., et al., Fatal immune restoration disease in human immunodeficiency virus type 1-infected patients with progressive multifocal leukoencephalopathy:impact of antiretroviral therapy-associated immune reconstitution. Clin Infect Dis,2002.35(10):p.1250-7.
[78]. Kwak, E.J., et al., Pathogenesis and management of polyomavirus infection in transplant recipients. Clin Infect Dis,2002.35(9):p.1081-7.
[79]. Vilchez, R.A., et al., Association between simian virus 40 and non-Hodgkin lymphoma. Lancet,2002.359(9309):p.817-23.
[80]. Boldorini, R., et al., Molecular characterization and sequence analysis of polyomavirus strains isolated from needle biopsy specimens of kidney allograft recipients. Am J Clin Pathol, 2001.116(4):p.489-94.
[81]. Hurault de Ligny, B., [Viral infections in renal transplantation]. Presse Med,2001.30(24 Pt 2):p.16-8.
[82]. Baksh, F.K., et al., Molecular genotyping of BK and JC viruses in human polyomavirus-associated interstitial nephritis after renal transplantation. Am J Kidney Dis, 2001.38(2):p.354-65.
[83]. Tognon, M., et al., Investigation of the simian polyomavirus SV40 as a potential causative agent of human neurological disorders in AIDS patients. J Med Microbiol,2001.50(2):p. 165-72.
[84]. Hurault de Ligny, B., et al., Polyomavirus-induced acute tubulo-interstitial nephritis in renal allograft recipients. Transplant Proc,2000.32(8):p.2760-1.
[85]. Altschuler, E.L., Viral inoculation for prophylaxis of polyoma virus associated diseases. Med Hypotheses,2001.56(1):p.24-5.
[86]. Bendiksen, S., et al., VP1 DNA sequences of JC and BK viruses detected in urine of systemic lupus erythematosus patients reveal no differences from strains expressed in normal individuals. J Gen Virol,2000.81(Pt 11):p.2625-33.
[87]. Fedele, C.G., et al., Multiplex polymerase chain reaction for the simultaneous detection and typing of polyomavirus JC, BK and SV40 DNA in clinical samples. J Virol Methods,1999. 82(2):p.137-44.
[88]. Shah, K.V., Human polyomavirus BKV and renal disease. Nephrol Dial Transplant,2000. 15(6):p.754-5.
[89]. Caldarelli-Stefano, R., et al., JC virus in human glial-derived tumors. Hum Pathol,2000. 31(3):p.394-5.
[90]. Weggen, S., et al., Low frequency of SV40, JC and BK polyomavirus sequences in human medulloblastomas, meningiomas and ependymomas. Brain Pathol,2000.10(1):p.85-92.
[91]. Imperiale, M.J., The human polyomaviruses, BKV and JCV:molecular pathogenesis of acute disease and potential role in cancer. Virology,2000.267(1):p.1-7.
[92]. Cubukcu-Dimopulo, O., et al., BK virus infection in AIDS. Am J Surg Pathol,2000.24(1):p. 145-9.
[93]. Hamilton, R.S., M. Gravell, and E.O. Major, Comparison of antibody titers determined by hemagglutination inhibition and enzyme immunoassay for JC virus and BK virus. J Clin Microbiol,2000.38(1):p.105-9.
[94]. Bofill-Mas, S., S. Pina, and R. Girones, Documenting the epidemiologic patterns of polyomaviruses in human populations by studying their presence in urban sewage. Appl Environ Microbiol,2000.66(1):p.238-45.
[95]. Huang, H., et al., Identification in human brain tumors of DNA sequences specific for SV40 large T antigen. Brain Pathol,1999.9(1):p.33-42.
[96]. Dorries, K., Molecular biology and pathogenesis of human polyomavirus infections. Dev Biol Stand,1998.94:p.71-9.
[97]. Galateau-Salle, F., et al., SV40-like DNA sequences in pleural mesothelioma, bronchopulmonary carcinoma, and non-malignant pulmonary diseases. J Pathol,1998.184(3): p.252-7.
[98]. Gallia, G.L., J. Gordon, and K. Khalili, Tumor pathogenesis of human neurotropic JC virus in the CNS. J Neurovirol,1998.4(2):p.175-81.
[99]. Tsai, R.T., et al., Incidence of JC viruria is higher than that of BK viruria in Taiwan. J Med Virol,1997.52(3):p.253-7.
[100]. Lednicky, J.A. and J.S. Butel, A coupled PCR and restriction digest method for the detection and analysis of the SV40 regulatory region in infected-cell lysates and clinical samples. J Virol Methods,1997.64(1):p.1-9.
[101]. Allander, T., et al., Identification of a third human polyomavirus. J Virol,2007.81(8):p. 4130-6.
[102]. Gaynor, C.H., et al., FPIN's clinical inquiries. Group medical visits for the management of chronic pain. Am Fam Physician,2007.76(11):p.1704-5.
[103]. Gaynor, J.W., J. Bentley, and R. Cosstick, Synthesis of the 3'-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage. Nat Protoc,2007.2(12):p.3122-35.
[104]. Gaynor, J.W., J. Brazier, and R. Cosstick, Synthesis of 3'-S-phosphorothiolate oligonucleotides for their potential use in RNA interference. Nucleosides Nucleotides Nucleic Acids,2007.26(6-7):p.709-12.
[105]. Gaynor, M., et al.,An intelligent pre-hospital patient care system. Int J Electron Healthc,2007. 3(1):p.107-22.
[106]. Lam, W.Y., et al., Survey for the presence of BK, JC, KI, WU and Merkel cell polyomaviruses in human brain tissues. J Clin Virol,2010.
[107]. Yu, F., et al., Tubulointerstitial lesions of patients with lupus nephritis classified by the 2003 International Society of Nephrology and Renal Pathology Society system. Kidney Int,2010.
[108]. Boothpur, R. and D.C. Brennan, Human polyoma viruses and disease with emphasis on clinical BK and JC. J Clin Virol,2010.47(4):p.306-12.
[109]. Debiaggi, M., et al., Molecular epidemiology of KI and WU polyomaviruses in infants with acute respiratory disease and in adult hematopoietic stem cell transplant recipients. J Med Virol,2010.82(1):p.153-6.
[110]. Barzon, L., et al., WU and KI polyomaviruses in the brains of HIV-positive patients with and without progressive multifocal leukoencephalopathy. J Infect Dis,2009.200(11):p.1755-8.
[111]. Mueller, A., et al., Polyomaviruses KI and WU in children with respiratory tract infection. Arch Virol,2009.154(10):p.1605-8.
[112]. Bergallo, M., et al., Real time PCR TaqMan assays for detection of polyomaviruses KIV and WUV in clinical samples. J Virol Methods,2009.162(1-2):p.69-74.
[113]. Babakir-Mina, M., et al., Excretion of the novel polyomaviruses KI and WU in the stool of patients with hematological disorders. J Med Virol,2009.81(9):p.1668-73.
[114]. Babakir-Mina, M., et al., Identification of the novel KI and WU polyomaviruses in human tonsils. J Clin Virol,2009.46(1):p.75-9.
[115]. Bialasiewicz, S., et al., Detection of BK, JC, WU, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples. J Clin Virol,2009.45(3):p.249-54.
[116]. Dalianis, T., et al., KI, WU and Merkel cell polyomaviruses:a new era for human polyomavirus research. Semin Cancer Biol,2009.19(4):p.270-5.
[117]. van de Pol, A.C., et al., Human bocavirus and KI/WU polyomaviruses in pediatric intensive care patients. Emerg Infect Dis,2009.15(3):p.454-7.
[118]. Venter, M., A. Visser, and R. Lassauniere, Human polyomaviruses, WU and KI in HIV exposed children with acute lower respiratory tract infections in hospitals in South Africa. J Clin Virol,2009.44(3):p.230-4.
[119]. Mourez, T., et al., Polyomaviruses KI and WU in immunocompromised patients with respiratory disease. Emerg Infect Dis,2009.15(1):p.107-9.
[120]. Sharp, C.P., et al., Reactivation and mutation of newly discovered WU, KI, and Merkel cell carcinoma polyomaviruses in immunosuppressed individuals. J Infect Dis,2009.199(3):p. 398-404.
[121]. Lindau, C., et al., A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses. J Clin Virol,2009.44(1):p.24-6.
[122]. van der Zalm, M.M., et al., Prevalence and pathogenicity of WU and KI polyomaviruses in children, the Netherlands. Emerg Infect Dis,2008.14(11):p.1787-9.
[123]. Lin, F., et al., [Identification of KI polyomavirus in children with lower respiratory tract infections from Zhejiang region of China]. Bing Du Xue Bao,2008.24(4):p.317-9.
[124]. Payungporn, S., et al., Detection and discrimination of WU/KI polyomaviruses by real-time PCR with melting curve analysis. J Virol Methods,2008.153(1):p.70-3.
[125]. Gu, S.X., et al., Re:Chang KW, Chang KI, Wu CM. Enhanced capacity for spontaneous correction of lumbar curve in the treatment of major thoracic-compensatory C modifier lumbar curve pattern in idiopathic scoliosis. Spine 2008;32:3020-9. Spine (Phila Pa 1976), 2008.33(13):p.1501; author reply 1501-2.
[126]. Payungporn, S., et al., Prevalence and molecular characterization of WU/KI polyomaviruses isolated from pediatric patients with respiratory disease in Thailand. Virus Res,2008.135(2): p.230-6.
[127]. Allander, T., et al., Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A,2005.102(36):p.12891-6.
[128]. Kantola, K., et al., Serodiagnosis of human bocavirus infection. Clin Infect Dis,2008.46(4):p. 540-6.
[129]. Allander, T., Human bocavirus. J Clin Virol,2008.41(1):p.29-33.
[130]. Koskenvuo, M., et al., Human bocavirus in children with acute lymphoblastic leukemia. Eur J Pediatr,2008.167(9):p.1011-5.
[131]. Allander, T., et al., Human bocavirus and acute wheezing in children. Clin Infect Dis,2007. 44(7):p.904-10.
[132]. Sloots, T.P., et al., Evidence of human coronavirus HKU1 and human bocavirus in Australian children. J Clin Virol,2006.35(1):p.99-102.
[133]. Zhao, L.Q., et al., Human bocavirus infections are common in Beijing population indicated by sero-antibody prevalence analysis. Chin Med J (Engl),2009.122(11):p.1289-92.
[134]. Ou, S.Y., et al., [Viral pathogens of acute lower respiratory tract infection in hospitalized children from East Guangdong of China]. Zhongguo Dang Dai Er Ke Za Zhi,2009.11(3):p. 203-6.
[135]. Sun, Y., et al., Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. J Virol,2009.83(8):p.3956-67.
[136]. Lin, J.H., et al., Clinical and genetic analysis of Human Bocavirus in children with lower respiratory tract infection in Taiwan. J Clin Virol,2009.44(3):p.219-24.
[137]. Zhao, L.Q., et al., [Seroprevalence of antibody against human bocavirus in Beijing, China]. Zhonghua Er Ke Za Zhi,2008.46(2):p.111-4.
[138]. Zhang, L.L., et al., Human bocavirus in children suffering from acute lower respiratory tract infection in Beijing Children's Hospital. Chin Med J (Engl),2008.121(17):p.1607-10.
[139]. Lin, F., et al., [Characterization of the cytopathic effect in human bronchial epithelial cell after Human Bocavirus Infection (HBoV)]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi,2008.22(2):p.107-9.
[140]. Cheng, W.X., et al., Human bocavirus in children hospitalized for acute gastroenteritis:a case-control study. Clin Infect Dis,2008.47(2):p.161-7.
[141]. Yu, J.M., et al., Human bocavirus infection in children hospitalized with acute gastroenteritis in China. J Clin Virol,2008.42(3):p.280-5.
[142]. Zhao, S., et al., Analysis of synonymous codon usage in 11 human bocavirus isolates. Biosystems,2008.92(3):p.207-14.
[143]. Zheng, M.Q., et al., [Clinical prospective study on maternal-fetal transmission of human bocavirus]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi,2007.21(4):p.331-3.
[144]. Zeng, M. and Q.R. Zhu, [Recent progress in studies on human bocavirus]. Zhonghua Er Ke Za Zhi,2007.45(9):p.678-80.
[145]. Qu, X.W., et al., Phospholipase A2-like activity of human bocavirus VP1 unique region. Biochem Biophys Res Commun,2008.365(1):p.158-63.
[146]. Qu, X.W., et al., Human bocavirus infection, People's Republic of China. Emerg Infect Dis, 2007.13(1):p.165-8.
[147]. Lu, X., et al., Real-time PCR assays for detection of bocavirus in human specimens. J Clin Microbiol,2006.44(9):p.3231-5.
[148]. Smuts, H. and D. Hardie, Human bocavirus in hospitalized children, South Africa. Emerg Infect Dis,2006.12(9):p.1457-8.
[149]. Garcia, M.L., et al., Detection of human bocavirus in ill and healthy Spanish children:a 2-year study. Arch Dis Child,2009.
[150]. Fry, A.M., et al., Human bocavirus:a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis,2007.195(7):p.1038-45.
[151]. Yoshida, L.M., et al., Viral pathogens associated with acute respiratory infections in central Vietnamese children. Pediatr Infect Dis J,2010.29(1):p.75-7.
[152]. Simmonds, P., Steps towards serological diagnosis of human bocavirus infections. Clin Infect Dis,2008.46(4):p.547-9.
[153]. Endo, R., et al., Seroepidemiology of human bocavirus in Hokkaido prefecture, Japan. J Clin Microbiol,2007.45(10):p.3218-23.
[154]. Clement, N., et al., Prevalence of human bocavirus in human tonsils and adenoids. Emerg Infect Dis,2009.15(7):p.1149-50.
[155]. Enders, M., et al., No detection of human bocavirus in amniotic fluid samples from fetuses with hydrops or isolated effusions. J Clin Virol,2009.45(4):p.300-3.
[156]. Kapoor, A., et al., A newly identified bocavirus species in human stool. J Infect Dis,2009. 199(2):p.196-200.
[157]. Victoria, J.G., et al., Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis. J Virol,2009.83(9):p.4642-51.
[158]. Lehmann, C., et al., Kawasaki disease lacks association with human coronavirus NL63 and human bocavirus. Pediatr Infect Dis J,2009.28(6):p.553-4.
[159]. Lu, X., L.R. Gooding, and D.D. Erdman, Human bocavirus in tonsillar lymphocytes. Emerg Infect Dis,2008.14(8):p.1332-4.
[160]. Rezes, S., et al., Human bocavirus and rhino-enteroviruses in childhood otitis media with effusion. J Clin Virol,2009.46(3):p.234-7.
[161]. Tozer, S.J., et al., Detection of human bocavirus in respiratory, fecal, and blood samples by real-time PCR. J Med Virol,2009.81(3):p.488-93.
[162]. Hengst, M., et al., [Human Bocavirus-infection (HBoV):an important cause of severe viral obstructive bronchitis in children]. Klin Padiatr,2008.220(5):p.296-301.
[163]. Chow, B.D. and F.P. Esper, The human bocaviruses:a review and discussion of their role in infection. Clin Lab Med,2009.29(4):p.695-713.
[164]. Lindner, J. and S. Modrow, Human bocavirus--a novel parvovirus to infect humans. Intervirology,2008.51(2):p.116-22.
[165]. Gwaltney, J.M., Jr., Virology and immunology of the common cold. Rhinology,1985.23(4):p. 265-71.
[166]. 刘国荣.申昆玲,江载芳,北京地区呼吸道合胞病毒感染的临床特点.实用儿科临床杂志,2002,17(3)：177-179.
[167]. 谢健屏,常汝需,何翠娟等,1978-1996年广州地区呼吸道合胞病毒感染分析.中华儿科杂志,1997,35(8)：402-404.
[168].李军,朱启镕，上海复旦大学附属儿科医院急性下呼吸道感染患儿常见病毒的检测及临床研究.微生物与感染,2006,1(4)：217-224.
[169]. 张荣芳,金玉,耿新林,刘宗燕,原新慧,急性呼吸道感染患儿副流感病毒3型检测及临床研究 中国实用儿科杂志,2008 23 (12)P.901-904.
[170]. Fishaut, J.M. and S.T. Mokrohisky, Cervical lymphadenitis caused by Haemophilus influenzae type B. Am J Dis Child,1977.131(8):p.925-6.
[171]. Arthur, J.L., et al., A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog,2009.5(4):p. e1000391.
[172]. Chow, B.D., Z. Ou, and F.P. Esper, Newly recognized bocaviruses (HBoV, HBoV2) in children and adults with gastrointestinal illness in the United States. J Clin Virol,2010.47(2): p.143-7.
[173]. Han, T.H., et al., Detection of human bocavirus-2 in children with acute gastroenteritis in South Korea. Arch Virol,2009.154(12):p.1923-7.
[174]. Han, T.H., J.Y. Chung, and E.S. Hwang, Human bocavirus 2 in children, South Korea. Emerg Infect Dis,2009.15(10):p.1698-700.
[175]. Song, J.R., et al., Novel human bocavirus in children with acute respiratory tract infection. Emerg Infect Dis.16(2):p.324-7.
[176]. Amar, C.F., et al., Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples:re-examination of the English case-control Infectious Intestinal Disease Study (1993-1996). Eur J Clin Microbiol Infect Dis,2007.26(5):p.311-23.
[2]. D'Angio, C.T., High altitude as an explanation for bronchiolitis-associated hospitalizations. Pediatr Infect Dis J,2000.19(5):p.492.
[3]. Lowther, S.A., et al., Bronchiolitis-associated hospitalizations among American Indian and Alaska Native children. Pediatr Infect Dis J,2000.19(1):p.11-7.
[4]. Shay, D.K., et al., Bronchiolitis-associated hospitalizations among US children,1980-1996. JAMA,1999.282(15):p.1440-6.
[5]. Stang, P., N. Brandenburg, and B. Carter, The economic burden of respiratory syncytial virus-associated bronchiolitis hospitalizations. Arch Pediatr Adolesc Med,2001.155(1):p. 95-6.
[6]. Medici, M.C., et al., Four year incidence of respiratory syncytial virus infection in infants and young children referred to emergency departments for lower respiratory tract diseases in Italy: the "Osservatorio VRS" Study (2000-2004). New Microbiol,2006.29(1):p.35-43.
[7]. Ventre, K. and A. Randolph, Ribavirin for respiratory syncytial virus infection of the lower respiratory tract in infants and young children. Cochrane Database Syst Rev,2004(4):p. CD000181.
[8]. van Woensel, J.B., W.M. van Aalderen, and J.L. Kimpen, Viral lower respiratory tract infection in infants and young children. BMJ,2003.327(7405):p.36-40.
[9]. van den Hoogen, B.G., et al., A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med,2001.7(6):p.719-24.
[10]. Bern, R.A., et al., Granzyme A- and B-cluster deficiency delays acute lung injury in pneumovirus-infected mice. J Immunol,2010.184(2):p.931-8.
[11]. Dyer, K.D., et al., Pneumoviruses infect eosinophils and elicit MyD88-dependent release of chemoattractant cytokines and interleukin-6. Blood,2009.114(13):p.2649-56.
[12]. Percopo, C.M., et al., Pulmonary eosinophils and their role in immunopathologic responses to formalin-inactivated pneumonia virus of mice. J Immunol,2009.183(1):p.604-12.
[13]. Tecu, C., et al., First detection of human metapneumovirus in children with respiratory infections in Romania. Roum Arch Microbiol Immunol,2007.66(1-2):p.37-40.
[14]. Broor, S. and P. Bharaj, Avian and human metapneumovirus. Ann N Y Acad Sci,2007.1102: p.66-85.
[15]. Kikuta, H., [Human metapneumovirus]. Uirusu,2006.56(2):p.173-81.
[16]. Farnsworth, J.E., et al., Development of a method for bacteria and virus recovery from heating, ventilation, and air conditioning (HVAC) filters. J Environ Monit,2006.8(10):p.1006-13.
[17]. van den Hoogen, B.G., et al., Antigenic and genetic variability of human metapneumoviruses. Emerg Infect Dis,2004.10(4):p.658-66.
[18]. Kapp, C., WHO lowers figures on SARS infections. Lancet,2003.362(9391):p.1207.
[19]. Lai, R.Q., et al., [Pathological and ultramicrostructural changes of tissues in a patient with severe acute respiratory syndrome]. Zhonghua Bing Li Xue Za Zhi,2003.32(3):p.205-8.
[20]. Lai, M.D., Y.M. Zhu, and X.M. Gu, [Severe acute respiratory syndrome]. Zhejiang Da Xue Xue Bao Yi Xue Ban,2003.32(3):p.167-70.
[21]. Kissoon, N., Severe acute respiratory syndrome:providing care while minimizing personal risks. Indian Pediatr,2003.40(7):p.645-51.
[22]. Ding, Y.Q., et al., [Study on etiology and pathology of severe acute respiratory syndrome]. Zhonghua Bing Li Xue Za Zhi,2003.32(3):p.195-200.
[23]. van der Hoek, L., et al., Identification of a new human coronavirus. Nat Med,2004.10(4):p. 368-73.
[24]. Kahn, J.S. and K. McIntosh, History and recent advances in coronavirus discovery. Pediatr Infect Dis J,2005.24(11 Suppl):p. S223-7, discussion S226.
[25]. Woo, P.C., et al., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J Virol,2005.79(2):p.884-95.
[26]. Respiratory syncytial virus activity--United States,2003-2004. MMWR Morb Mortal Wkly Rep,2004.53(49):p.1159-60.
[27]. Goldrick, B.A., Emerging infections:respiratory syncytial virus. Am J Nurs,2004.104(11):p. 54-6.
[28]. Valarcher, J.F. and G. Taylor, Bovine respiratory syncytial virus infection. Vet Res,2007. 38(2):p.153-80.
[29]. Rosenberg, H.F., et al., The pneumonia virus of mice infection model for severe respiratory syncytial virus infection:identifying novel targets for therapeutic intervention. Pharmacol Ther,2005.105(1):p.1-6.
[30]. Kho, N., et al., Respiratory syncytial virus infection and neurologic abnormalities: retrospective cohort study. J Child Neurol,2004.19(11):p.859-64.
[31]. Walls, T., et al., Polymerase chain reaction in the management of disseminated adenovirus infection after stem cell transplantation. Pediatr Infect Dis J,2004.23(12):p.1167-9.
[32]. McConnell, M.J. and M.J. Imperiale, Biology of adenovirus and its use as a vector for gene therapy. Hum Gene Ther,2004.15(11):p.1022-33.
[33]. Adenovirus. Am J Transplant,2004.4 Suppl 10:p.101-4.
[34]. Li, L., et al., Endostatin gene therapy for liver cancer by a recombinant adenovirus delivery. World J Gastroenterol,2004.10(13):p.1867-71.
[35]. Fleury, S., et al., Helper-dependent adenovirus vectors devoid of all viral genes cause less myocardial inflammation compared with first-generation adenovirus vectors. Basic Res Cardiol,2004.99(4):p.247-56.
[36]. Cuesta, R., Q. Xi, and R.J. Schneider, Structural basis for competitive inhibition of eIF4G-Mnkl interaction by the adenovirus 100-kilodalton protein. J Virol,2004.78(14):p. 7707-16.
[37]. Ostlund, M.R., et al., Respiratory virus infections in Stockholm during seven seasons:a retrospective study of laboratory diagnosis. Scand J Infect Dis,2004.36(6-7):p.460-5.
[38]. Monto, A.S., Occurrence of respiratory virus:time, place and person. Pediatr Infect Dis J, 2004.23(1 Suppl):p. S58-64.
[39]. Ngama, M.J., et al., Comparison of three methods of collecting nasal specimens for respiratory virus analysis. East Afr Med J,2004.81(6):p.313-7.
[40]. Dunn, J.J., et al., Sensitivity of respiratory virus culture when screening with R-mix fresh cells. J Clin Microbiol,2004.42(1):p.79-82.
[41]. Abadesso, C., et al., Use of palivizumab to control an outbreak of syncytial respiratory virus in a neonatal intensive care unit. J Hosp Infect,2004.58(1):p.38-41.
[42]. Riedel, F., et al., Role of sensory neuropeptides in PIV-3-infection-induced airway hyperresponsiveness in guinea pigs. Respiration,1997.64(3):p.211-9.
[43]. Karron, R.A., et al., A live human parainfluenza type 3 virus vaccine is attenuated and immunogenic in healthy infants and children. J Infect Dis,1995.172(6):p.1445-50.
[44]. Karron, R.A., et al., Rapid detection of parainfluenza virus type 3 RNA in respiratory specimens:use of reverse transcription-PCR-enzyme immunoassay. J Clin Microbiol,1994. 32(2):p.484-8.
[45]. Falsey, A.R., et al., Long-term care facilities:a cornucopia of viral pathogens. J Am Geriatr Soc,2008.56(7):p.1281-5.
[46]. Hagglund, S., et al., A six-year study on respiratory viral infections in a bull testing facility. Vet J,2007.173(3):p.585-93.
[47]. Ding, Y., et al., Organ distribution of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in SARS patients:implications for pathogenesis and virus transmission pathways. J Pathol,2004.203(2):p.622-30.
[48]. Wu, X.D., et al., The spike protein of severe acute respiratory syndrome (SARS) is cleaved in virus infected Vero-E6 cells. Cell Res,2004.14(5):p.400-6.
[49]. Midulla, F., et al., Respiratory syncytial virus, human bocavirus and rhinovirus bronchiolitis in infants. Arch Dis Child,2010.95(1):p.35-41.
[50]. Bacha, U., et al., Development of broad-spectrum halomethyl ketone inhibitors against coronavirus main protease 3CL(pro). Chem Biol Drug Des,2008.72(1):p.34-49.
[51]. Gagneur, A., et al., Materno-fetal transmission of human coronaviruses:a prospective pilot study. Eur J Clin Microbiol Infect Dis,2008.27(9):p.863-6.
[52]. Rowley, A.H. and S.T. Shulman, New developments in the search for the etiologic agent of Kawasaki disease. Curr Opin Pediatr,2007.19(1):p.71-4.
[53]. Bastien, N., et al., Human coronavirus NL-63 infections in children:a 1-year study. J Clin Microbiol,2005.43(9):p.4567-73.
[54]. Woo, P.C., et al., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia. J Infect Dis,2005.192(11):p.1898-907.
[55]. Woo, P.C., et al., Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia. Arch Virol,2005.150(11):p.2299-311.
[56]. Woo, P.C., et al., In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease. Microbiol Immunol,2005. 49(10):p.899-908.
[57]. Esper, F., Z. Ou, and Y.T. Huang, Human coronaviruses are uncommon in patients with gastrointestinal illness. J Clin Virol,2010.
[58]. Leung, T.F., et al., Epidemiology and clinical presentations of human coronavirus NL63 infections in hong kong children. J Clin Microbiol,2009.47(11):p.3486-92.
[59]. Chan, C.M., et al., Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus. J Clin Virol,2009.45(1):p.54-60.
[60]. Vijgen, L., et al., A pancoronavirus RT-PCR assay for detection of all known coronaviruses. Methods Mol Biol,2008.454:p.3-12.
[61]. Chung, J.Y., et al., Detection of viruses identified recently in children with acute wheezing. J Med Virol,2007.79(8):p.1238-43.
[62]. Pyrc, K., B. Berkhout, and L. van der Hoek, Identification of new human coronaviruses. Expert Rev Anti Infect Their,2007.5(2):p.245-53.
[63]. Pyrc, K., B. Berkhout, and L. van der Hoek, Antiviral strategies against human coronaviruses. Infect Disord Drug Targets,2007.7(1):p.59-66.
[64]. Osborn, J.E., et al., Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments. J Virol, 1974.13(3):p.614-22.
[65]. Galateau-Salle, F., et al., Detection of SV40-like DNA sequences in pleural mesothelioma, bronchopulmonary carcinoma and other pulmonary diseases. Dev Biol Stand,1998.94:p. 147-52.
[66]. Dorries, K., New aspects in the pathogenesis of polyomavirus-induced disease. Adv Virus Res, 1997.48:p.205-61.
[67]. Vago, L., et al., JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol,1996.12(2):p.139-46.
[68]. Miyamura, T., K. Yoshiike, and K.K. Takemoto, High-titer SV40 replication in human fibroblast cell lines derived from patients with Wiskott-Aldrich syndrome. Virology,1983. 129(2):p.479-83.
[69]. Hogan, T.F., et al., Survey of human polyomavirus (JCV, BKV) infections in 139 patients with lung cancer, breast cancer, melanoma, or lymphoma. Prog Clin Biol Res,1983.105:p. 311-24.
[70]. Hogan, T.F., et al., Human polyomavirus infections with JC virus and BK virus in renal transplant patients. Ann Intern Med,1980.92(3):p.373-8.
[71]. Hogan, T.F., et al., Rapid detection and identification of JC virus and BK virus in human urine by using immunofluorescence microscopy. J Clin Microbiol,1980.11(2):p.178-83.
[72]. Shah, K.V., et al., Investigation of human urogenital tract tumors of papovavirus etiology: brief communication. J Natl Cancer Inst,1978.60(3):p.579-82.
[73]. Endo, S., et al., JC virus agnoprotein colocalizes with tubulin. J Neurovirol,2003.9 Suppl 1: p.10-4.
[74]. Knoll, A., et al., Low frequency of human polyomavirus BKV and JCV DNA in urothelial carcinomas of the renal pelvis and renal cell carcinomas. Oncol Rep,2003.10(2):p.487-91.
[75]. Li, R.M., et al., BK virus and SV40 co-infection in polyomavirus nephropathy. Transplantation, 2002.74(11):p.1497-504.
[76]. Zambrano, A., et al., Detection of human polyomaviruses and papillomaviruses in prostatic tissue reveals the prostate as a habitat for multiple viral infections. Prostate,2002.53(4):p. 263-76.
[77]. Safdar, A., et al., Fatal immune restoration disease in human immunodeficiency virus type 1-infected patients with progressive multifocal leukoencephalopathy:impact of antiretroviral therapy-associated immune reconstitution. Clin Infect Dis,2002.35(10):p.1250-7.
[78]. Kwak, E.J., et al., Pathogenesis and management of polyomavirus infection in transplant recipients. Clin Infect Dis,2002.35(9):p.1081-7.
[79]. Vilchez, R.A., et al., Association between simian virus 40 and non-Hodgkin lymphoma. Lancet,2002.359(9309):p.817-23.
[80]. Boldorini, R., et al., Molecular characterization and sequence analysis of polyomavirus strains isolated from needle biopsy specimens of kidney allograft recipients. Am J Clin Pathol, 2001.116(4):p.489-94.
[81]. Hurault de Ligny, B., [Viral infections in renal transplantation]. Presse Med,2001.30(24 Pt 2):p.16-8.
[82]. Baksh, F.K., et al., Molecular genotyping of BK and JC viruses in human polyomavirus-associated interstitial nephritis after renal transplantation. Am J Kidney Dis, 2001.38(2):p.354-65.
[83]. Tognon, M., et al., Investigation of the simian polyomavirus SV40 as a potential causative agent of human neurological disorders in AIDS patients. J Med Microbiol,2001.50(2):p. 165-72.
[84]. Hurault de Ligny, B., et al., Polyomavirus-induced acute tubulo-interstitial nephritis in renal allograft recipients. Transplant Proc,2000.32(8):p.2760-1.
[85]. Altschuler, E.L., Viral inoculation for prophylaxis of polyoma virus associated diseases. Med Hypotheses,2001.56(1):p.24-5.
[86]. Bendiksen, S., et al., VP1 DNA sequences of JC and BK viruses detected in urine of systemic lupus erythematosus patients reveal no differences from strains expressed in normal individuals. J Gen Virol,2000.81(Pt 11):p.2625-33.
[87]. Fedele, C.G., et al., Multiplex polymerase chain reaction for the simultaneous detection and typing of polyomavirus JC, BK and SV40 DNA in clinical samples. J Virol Methods,1999. 82(2):p.137-44.
[88]. Shah, K.V., Human polyomavirus BKV and renal disease. Nephrol Dial Transplant,2000. 15(6):p.754-5.
[89]. Caldarelli-Stefano, R., et al., JC virus in human glial-derived tumors. Hum Pathol,2000. 31(3):p.394-5.
[90]. Weggen, S., et al., Low frequency of SV40, JC and BK polyomavirus sequences in human medulloblastomas, meningiomas and ependymomas. Brain Pathol,2000.10(1):p.85-92.
[91]. Imperiale, M.J., The human polyomaviruses, BKV and JCV:molecular pathogenesis of acute disease and potential role in cancer. Virology,2000.267(1):p.1-7.
[92]. Cubukcu-Dimopulo, O., et al., BK virus infection in AIDS. Am J Surg Pathol,2000.24(1):p. 145-9.
[93]. Hamilton, R.S., M. Gravell, and E.O. Major, Comparison of antibody titers determined by hemagglutination inhibition and enzyme immunoassay for JC virus and BK virus. J Clin Microbiol,2000.38(1):p.105-9.
[94]. Bofill-Mas, S., S. Pina, and R. Girones, Documenting the epidemiologic patterns of polyomaviruses in human populations by studying their presence in urban sewage. Appl Environ Microbiol,2000.66(1):p.238-45.
[95]. Huang, H., et al., Identification in human brain tumors of DNA sequences specific for SV40 large T antigen. Brain Pathol,1999.9(1):p.33-42.
[96]. Dorries, K., Molecular biology and pathogenesis of human polyomavirus infections. Dev Biol Stand,1998.94:p.71-9.
[97]. Galateau-Salle, F., et al., SV40-like DNA sequences in pleural mesothelioma, bronchopulmonary carcinoma, and non-malignant pulmonary diseases. J Pathol,1998.184(3): p.252-7.
[98]. Gallia, G.L., J. Gordon, and K. Khalili, Tumor pathogenesis of human neurotropic JC virus in the CNS. J Neurovirol,1998.4(2):p.175-81.
[99]. Tsai, R.T., et al., Incidence of JC viruria is higher than that of BK viruria in Taiwan. J Med Virol,1997.52(3):p.253-7.
[100]. Lednicky, J.A. and J.S. Butel, A coupled PCR and restriction digest method for the detection and analysis of the SV40 regulatory region in infected-cell lysates and clinical samples. J Virol Methods,1997.64(1):p.1-9.
[101]. Allander, T., et al., Identification of a third human polyomavirus. J Virol,2007.81(8):p. 4130-6.
[102]. Gaynor, C.H., et al., FPIN's clinical inquiries. Group medical visits for the management of chronic pain. Am Fam Physician,2007.76(11):p.1704-5.
[103]. Gaynor, J.W., J. Bentley, and R. Cosstick, Synthesis of the 3'-thio-nucleosides and subsequent automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage. Nat Protoc,2007.2(12):p.3122-35.
[104]. Gaynor, J.W., J. Brazier, and R. Cosstick, Synthesis of 3'-S-phosphorothiolate oligonucleotides for their potential use in RNA interference. Nucleosides Nucleotides Nucleic Acids,2007.26(6-7):p.709-12.
[105]. Gaynor, M., et al.,An intelligent pre-hospital patient care system. Int J Electron Healthc,2007. 3(1):p.107-22.
[106]. Lam, W.Y., et al., Survey for the presence of BK, JC, KI, WU and Merkel cell polyomaviruses in human brain tissues. J Clin Virol,2010.
[107]. Yu, F., et al., Tubulointerstitial lesions of patients with lupus nephritis classified by the 2003 International Society of Nephrology and Renal Pathology Society system. Kidney Int,2010.
[108]. Boothpur, R. and D.C. Brennan, Human polyoma viruses and disease with emphasis on clinical BK and JC. J Clin Virol,2010.47(4):p.306-12.
[109]. Debiaggi, M., et al., Molecular epidemiology of KI and WU polyomaviruses in infants with acute respiratory disease and in adult hematopoietic stem cell transplant recipients. J Med Virol,2010.82(1):p.153-6.
[110]. Barzon, L., et al., WU and KI polyomaviruses in the brains of HIV-positive patients with and without progressive multifocal leukoencephalopathy. J Infect Dis,2009.200(11):p.1755-8.
[111]. Mueller, A., et al., Polyomaviruses KI and WU in children with respiratory tract infection. Arch Virol,2009.154(10):p.1605-8.
[112]. Bergallo, M., et al., Real time PCR TaqMan assays for detection of polyomaviruses KIV and WUV in clinical samples. J Virol Methods,2009.162(1-2):p.69-74.
[113]. Babakir-Mina, M., et al., Excretion of the novel polyomaviruses KI and WU in the stool of patients with hematological disorders. J Med Virol,2009.81(9):p.1668-73.
[114]. Babakir-Mina, M., et al., Identification of the novel KI and WU polyomaviruses in human tonsils. J Clin Virol,2009.46(1):p.75-9.
[115]. Bialasiewicz, S., et al., Detection of BK, JC, WU, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples. J Clin Virol,2009.45(3):p.249-54.
[116]. Dalianis, T., et al., KI, WU and Merkel cell polyomaviruses:a new era for human polyomavirus research. Semin Cancer Biol,2009.19(4):p.270-5.
[117]. van de Pol, A.C., et al., Human bocavirus and KI/WU polyomaviruses in pediatric intensive care patients. Emerg Infect Dis,2009.15(3):p.454-7.
[118]. Venter, M., A. Visser, and R. Lassauniere, Human polyomaviruses, WU and KI in HIV exposed children with acute lower respiratory tract infections in hospitals in South Africa. J Clin Virol,2009.44(3):p.230-4.
[119]. Mourez, T., et al., Polyomaviruses KI and WU in immunocompromised patients with respiratory disease. Emerg Infect Dis,2009.15(1):p.107-9.
[120]. Sharp, C.P., et al., Reactivation and mutation of newly discovered WU, KI, and Merkel cell carcinoma polyomaviruses in immunosuppressed individuals. J Infect Dis,2009.199(3):p. 398-404.
[121]. Lindau, C., et al., A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses. J Clin Virol,2009.44(1):p.24-6.
[122]. van der Zalm, M.M., et al., Prevalence and pathogenicity of WU and KI polyomaviruses in children, the Netherlands. Emerg Infect Dis,2008.14(11):p.1787-9.
[123]. Lin, F., et al., [Identification of KI polyomavirus in children with lower respiratory tract infections from Zhejiang region of China]. Bing Du Xue Bao,2008.24(4):p.317-9.
[124]. Payungporn, S., et al., Detection and discrimination of WU/KI polyomaviruses by real-time PCR with melting curve analysis. J Virol Methods,2008.153(1):p.70-3.
[125]. Gu, S.X., et al., Re:Chang KW, Chang KI, Wu CM. Enhanced capacity for spontaneous correction of lumbar curve in the treatment of major thoracic-compensatory C modifier lumbar curve pattern in idiopathic scoliosis. Spine 2008;32:3020-9. Spine (Phila Pa 1976), 2008.33(13):p.1501; author reply 1501-2.
[126]. Payungporn, S., et al., Prevalence and molecular characterization of WU/KI polyomaviruses isolated from pediatric patients with respiratory disease in Thailand. Virus Res,2008.135(2): p.230-6.
[127]. Allander, T., et al., Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A,2005.102(36):p.12891-6.
[128]. Kantola, K., et al., Serodiagnosis of human bocavirus infection. Clin Infect Dis,2008.46(4):p. 540-6.
[129]. Allander, T., Human bocavirus. J Clin Virol,2008.41(1):p.29-33.
[130]. Koskenvuo, M., et al., Human bocavirus in children with acute lymphoblastic leukemia. Eur J Pediatr,2008.167(9):p.1011-5.
[131]. Allander, T., et al., Human bocavirus and acute wheezing in children. Clin Infect Dis,2007. 44(7):p.904-10.
[132]. Sloots, T.P., et al., Evidence of human coronavirus HKU1 and human bocavirus in Australian children. J Clin Virol,2006.35(1):p.99-102.
[133]. Zhao, L.Q., et al., Human bocavirus infections are common in Beijing population indicated by sero-antibody prevalence analysis. Chin Med J (Engl),2009.122(11):p.1289-92.
[134]. Ou, S.Y., et al., [Viral pathogens of acute lower respiratory tract infection in hospitalized children from East Guangdong of China]. Zhongguo Dang Dai Er Ke Za Zhi,2009.11(3):p. 203-6.
[135]. Sun, Y., et al., Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. J Virol,2009.83(8):p.3956-67.
[136]. Lin, J.H., et al., Clinical and genetic analysis of Human Bocavirus in children with lower respiratory tract infection in Taiwan. J Clin Virol,2009.44(3):p.219-24.
[137]. Zhao, L.Q., et al., [Seroprevalence of antibody against human bocavirus in Beijing, China]. Zhonghua Er Ke Za Zhi,2008.46(2):p.111-4.
[138]. Zhang, L.L., et al., Human bocavirus in children suffering from acute lower respiratory tract infection in Beijing Children's Hospital. Chin Med J (Engl),2008.121(17):p.1607-10.
[139]. Lin, F., et al., [Characterization of the cytopathic effect in human bronchial epithelial cell after Human Bocavirus Infection (HBoV)]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi,2008.22(2):p.107-9.
[140]. Cheng, W.X., et al., Human bocavirus in children hospitalized for acute gastroenteritis:a case-control study. Clin Infect Dis,2008.47(2):p.161-7.
[141]. Yu, J.M., et al., Human bocavirus infection in children hospitalized with acute gastroenteritis in China. J Clin Virol,2008.42(3):p.280-5.
[142]. Zhao, S., et al., Analysis of synonymous codon usage in 11 human bocavirus isolates. Biosystems,2008.92(3):p.207-14.
[143]. Zheng, M.Q., et al., [Clinical prospective study on maternal-fetal transmission of human bocavirus]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi,2007.21(4):p.331-3.
[144]. Zeng, M. and Q.R. Zhu, [Recent progress in studies on human bocavirus]. Zhonghua Er Ke Za Zhi,2007.45(9):p.678-80.
[145]. Qu, X.W., et al., Phospholipase A2-like activity of human bocavirus VP1 unique region. Biochem Biophys Res Commun,2008.365(1):p.158-63.
[146]. Qu, X.W., et al., Human bocavirus infection, People's Republic of China. Emerg Infect Dis, 2007.13(1):p.165-8.
[147]. Lu, X., et al., Real-time PCR assays for detection of bocavirus in human specimens. J Clin Microbiol,2006.44(9):p.3231-5.
[148]. Smuts, H. and D. Hardie, Human bocavirus in hospitalized children, South Africa. Emerg Infect Dis,2006.12(9):p.1457-8.
[149]. Garcia, M.L., et al., Detection of human bocavirus in ill and healthy Spanish children:a 2-year study. Arch Dis Child,2009.
[150]. Fry, A.M., et al., Human bocavirus:a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis,2007.195(7):p.1038-45.
[151]. Yoshida, L.M., et al., Viral pathogens associated with acute respiratory infections in central Vietnamese children. Pediatr Infect Dis J,2010.29(1):p.75-7.
[152]. Simmonds, P., Steps towards serological diagnosis of human bocavirus infections. Clin Infect Dis,2008.46(4):p.547-9.
[153]. Endo, R., et al., Seroepidemiology of human bocavirus in Hokkaido prefecture, Japan. J Clin Microbiol,2007.45(10):p.3218-23.
[154]. Clement, N., et al., Prevalence of human bocavirus in human tonsils and adenoids. Emerg Infect Dis,2009.15(7):p.1149-50.
[155]. Enders, M., et al., No detection of human bocavirus in amniotic fluid samples from fetuses with hydrops or isolated effusions. J Clin Virol,2009.45(4):p.300-3.
[156]. Kapoor, A., et al., A newly identified bocavirus species in human stool. J Infect Dis,2009. 199(2):p.196-200.
[157]. Victoria, J.G., et al., Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis. J Virol,2009.83(9):p.4642-51.
[158]. Lehmann, C., et al., Kawasaki disease lacks association with human coronavirus NL63 and human bocavirus. Pediatr Infect Dis J,2009.28(6):p.553-4.
[159]. Lu, X., L.R. Gooding, and D.D. Erdman, Human bocavirus in tonsillar lymphocytes. Emerg Infect Dis,2008.14(8):p.1332-4.
[160]. Rezes, S., et al., Human bocavirus and rhino-enteroviruses in childhood otitis media with effusion. J Clin Virol,2009.46(3):p.234-7.
[161]. Tozer, S.J., et al., Detection of human bocavirus in respiratory, fecal, and blood samples by real-time PCR. J Med Virol,2009.81(3):p.488-93.
[162]. Hengst, M., et al., [Human Bocavirus-infection (HBoV):an important cause of severe viral obstructive bronchitis in children]. Klin Padiatr,2008.220(5):p.296-301.
[163]. Chow, B.D. and F.P. Esper, The human bocaviruses:a review and discussion of their role in infection. Clin Lab Med,2009.29(4):p.695-713.
[164]. Lindner, J. and S. Modrow, Human bocavirus--a novel parvovirus to infect humans. Intervirology,2008.51(2):p.116-22.
[165]. Gwaltney, J.M., Jr., Virology and immunology of the common cold. Rhinology,1985.23(4):p. 265-71.
[166]. 刘国荣.申昆玲,江载芳,北京地区呼吸道合胞病毒感染的临床特点.实用儿科临床杂志,2002,17(3)：177-179.
[167]. 谢健屏,常汝需,何翠娟等,1978-1996年广州地区呼吸道合胞病毒感染分析.中华儿科杂志,1997,35(8)：402-404.
[168].李军,朱启镕，上海复旦大学附属儿科医院急性下呼吸道感染患儿常见病毒的检测及临床研究.微生物与感染,2006,1(4)：217-224.
[169]. 张荣芳,金玉,耿新林,刘宗燕,原新慧,急性呼吸道感染患儿副流感病毒3型检测及临床研究 中国实用儿科杂志,2008 23 (12)P.901-904.
[170]. Fishaut, J.M. and S.T. Mokrohisky, Cervical lymphadenitis caused by Haemophilus influenzae type B. Am J Dis Child,1977.131(8):p.925-6.
[171]. Arthur, J.L., et al., A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog,2009.5(4):p. e1000391.
[172]. Chow, B.D., Z. Ou, and F.P. Esper, Newly recognized bocaviruses (HBoV, HBoV2) in children and adults with gastrointestinal illness in the United States. J Clin Virol,2010.47(2): p.143-7.
[173]. Han, T.H., et al., Detection of human bocavirus-2 in children with acute gastroenteritis in South Korea. Arch Virol,2009.154(12):p.1923-7.
[174]. Han, T.H., J.Y. Chung, and E.S. Hwang, Human bocavirus 2 in children, South Korea. Emerg Infect Dis,2009.15(10):p.1698-700.
[175]. Song, J.R., et al., Novel human bocavirus in children with acute respiratory tract infection. Emerg Infect Dis.16(2):p.324-7.
[176]. Amar, C.F., et al., Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples:re-examination of the English case-control Infectious Intestinal Disease Study (1993-1996). Eur J Clin Microbiol Infect Dis,2007.26(5):p.311-23.