高速逆流色谱法分离药用植物锁阳中多酚类化合物
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摘要
锁阳(Cynomorium songaricum Rupr.),为多年生全寄生草本植物,寄生于蒺藜科(Zygophyllaceae)白刺属(Nitraria.L.)植物根部,主要分布在内蒙古、宁夏、新疆、甘肃、青海等地。锁阳富含多酚类物质,多酚类化合物具有较强的抗氧化作用,以及明显的抑菌、抗癌、抗老化和抑制胆固醇上升等功效。本研究采用高速逆流色谱法分离纯化锁阳中的多酚类化合物,这将为锁阳多酚类化合物的药理学研究提供有利条件,也可为锁阳资源的进一步市场开发提供一定的技术支持。
本研究通过高速逆流色谱对锁阳乙酸乙酯萃取部分进行分离纯化,采用正己烷-乙酸乙酯-甲醇-水(1:3:1:3 v/v)作为溶剂体系分离得到化合物Ⅱ,纯度为94.5%;以正己烷-乙酸乙酯-甲醇-水(1:20:2:20 v/v)为溶剂体系分离得到化合物Ⅲ(18mg)、化合物Ⅳ(6.4mg)和化合物Ⅴ(11.2mg),其纯度分别为98.1%、97.4%、98.6%;本研究在以正己烷-乙酸乙酯-甲醇-水(1:20:2:20 v/v)为溶剂体系分离化合物Ⅲ、Ⅳ、Ⅴ时,将各相间的物质峰收集到一起并在相同色谱条件下进行第二次分离,大大提高了分离度,取得较好的分离纯化效果,得到化合物Ⅵ、Ⅶ,纯度分别为97.1%和91.1%。此外,本研究还通过硅胶柱层析法分离得到化合物Ⅰ,其纯度为98.5%。
Cynomorium songaricum Rupr.is a full-parasitic plant.It distributes in Inner Mongolia,Ningxia,Xinjiang,Gansu and Qinghai provinces and parasites in roots of Zygophyllaceae Nitraria.Polyphenols is one of the mainly components of C.songaricum which have strong effects on anti-oxidant,anti-bacterial,anti-cancer, anti-aging and inhibiting the effectiveness of cholesterol rise.This study separated and purified C.songaricum polyphenols using high-speed countercurrent chromatography to provide advantages for Pharmacology and offer technology supports for development of C.songaricum resources.
This study separated extraction of ethyl acetate of C.songaricum using HSCCC with the solvent system hexane-ethyl acetate-methanol-water(1:3:1:3 v / v) and got compoundsⅡ,a purity of 94.5%;but got compoundsⅢ(18 mg)Ⅳ(6.4 rag)andⅤ(11.2 mg) with the solvent system hexane-ethyl acetate-methanol-water (1:20:2:20 v / v),which purity is 98.1%、97.4%、98.6%respectively.Furthermore, the study collected interval peaks of spectrum of HSCCC with the solvent system hexane-ethyl acetate-methanol-water(1:20:2:20 v/v),separated the two parts with the same solvent system respectively and got compoundsⅥandⅦ,which purity is 97.1%and 91.1%.In addition,the study also separated compoundsⅠusing silica gel column,which purity is 98.5%.
本研究通过高速逆流色谱对锁阳乙酸乙酯萃取部分进行分离纯化,采用正己烷-乙酸乙酯-甲醇-水(1:3:1:3 v/v)作为溶剂体系分离得到化合物Ⅱ,纯度为94.5%;以正己烷-乙酸乙酯-甲醇-水(1:20:2:20 v/v)为溶剂体系分离得到化合物Ⅲ(18mg)、化合物Ⅳ(6.4mg)和化合物Ⅴ(11.2mg),其纯度分别为98.1%、97.4%、98.6%;本研究在以正己烷-乙酸乙酯-甲醇-水(1:20:2:20 v/v)为溶剂体系分离化合物Ⅲ、Ⅳ、Ⅴ时,将各相间的物质峰收集到一起并在相同色谱条件下进行第二次分离,大大提高了分离度,取得较好的分离纯化效果,得到化合物Ⅵ、Ⅶ,纯度分别为97.1%和91.1%。此外,本研究还通过硅胶柱层析法分离得到化合物Ⅰ,其纯度为98.5%。
Cynomorium songaricum Rupr.is a full-parasitic plant.It distributes in Inner Mongolia,Ningxia,Xinjiang,Gansu and Qinghai provinces and parasites in roots of Zygophyllaceae Nitraria.Polyphenols is one of the mainly components of C.songaricum which have strong effects on anti-oxidant,anti-bacterial,anti-cancer, anti-aging and inhibiting the effectiveness of cholesterol rise.This study separated and purified C.songaricum polyphenols using high-speed countercurrent chromatography to provide advantages for Pharmacology and offer technology supports for development of C.songaricum resources.
This study separated extraction of ethyl acetate of C.songaricum using HSCCC with the solvent system hexane-ethyl acetate-methanol-water(1:3:1:3 v / v) and got compoundsⅡ,a purity of 94.5%;but got compoundsⅢ(18 mg)Ⅳ(6.4 rag)andⅤ(11.2 mg) with the solvent system hexane-ethyl acetate-methanol-water (1:20:2:20 v / v),which purity is 98.1%、97.4%、98.6%respectively.Furthermore, the study collected interval peaks of spectrum of HSCCC with the solvent system hexane-ethyl acetate-methanol-water(1:20:2:20 v/v),separated the two parts with the same solvent system respectively and got compoundsⅥandⅦ,which purity is 97.1%and 91.1%.In addition,the study also separated compoundsⅠusing silica gel column,which purity is 98.5%.
引文
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