水稻品种对条纹叶枯病抗性遗传的研究
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摘要
本研究通过选取江苏省主栽粳稻品种,鉴定其对条纹叶枯的抗性,并通过SSR标记对抗条纹叶枯基因进行定位,研究结果如下:
1.选用江苏生产推广使用的粳稻品种镇稻88、淮优粳1号、扬粳鉴7号及IR24(为对照),进行抗病性和抗虫性鉴定,结果表明:淮优粳1号表现为中抗,具有非嗜性和抗生性两个抗虫特性:武育粳3号表现为感病,不具有非嗜性和抗生性两个抗虫特性;镇稻88和扬粳鉴7分别表现为抗病和中抗,具有中等非嗜性特性和抗生性特性;IR24表现为中抗,具有中等非嗜性特性和抗生性特性。
2.利用抗病品种镇稻88、淮优粳1号、扬粳鉴7与感病品种武育粳3号配制抗感杂交组合,通过室内接种鉴定,研究了对条纹叶枯的抗性遗传。遗传分析表明:镇稻88、淮优粳1号、扬粳鉴7对条纹叶枯的抗性均由1对显性核基因控制,不受细胞质遗传的影响。
3.选用镇稻88、淮优粳1号、扬粳鉴7等3个抗病品种为亲本,通过品种间进行杂交,统计F_1和F_2的发病率,来确定不同抗病品种之间的等位性。研究结果表明:镇稻88、淮优粳1号、扬粳鉴7这3个品种的抗病基因是等位的,或者是紧密连锁的。
4.利用抗病品种镇稻88与感病品种武育粳3号配制F_2群体,采用水稻条纹叶枯病单分蘗鉴定法与苗期接种鉴定的方法分别对F_2群体、F_(2:3)家系进行抗病性鉴定,并根据F_(2:3)家系的抗病性表现推测相应F_2单株的基因型。结果显示:群体对条纹叶枯病抗性属于质量性状遗传,并且由一对主基因控制。水稻条纹叶枯病单分蘗鉴定法鉴定的结果与苗期抗性鉴定的结果是一致的。
利用MAPMAKER/3.0软件对F_2作图群体进行分子标记和抗病基因之间连锁分析。在所选的21对SSR引物中,有4对引物的PCR产物在镇稻88与武育粳3号两个亲本之间存在多态,多态性指数分别为19.0%。找到了与抗条纹叶枯基因连锁的SSR标记RM229。SSR标记RM229与抗病基因对遗传距离为4.7cM,位于第11染色体的一侧。
利用RAPD标记OPO11,在亲本镇稻88、武育粳3号进行PCR扩增,用0.8%的琼脂糖进行检测,发现镇稻88中有一个特异性条带,片段大约为2500bp。用该引物对F_2分离群体的200个单株的DNA进行PCR扩增,统计结果,用MAPMAKER/EXP3.0软件计算遗传距离,结果表明RAPD标记OPO11与抗病基因的遗传距离为0cM,表现为共分离。推测此抗病基因与stvb-i是同一个位点。
5.利用抗病品种宁317与感病品种武育粳3号配制F_2群体,采用水稻条纹叶枯病单分蘗鉴定法与苗期接种鉴定的方法分别对F_2群体、F_(2:3)家系进行抗病性鉴定,并根据F_(2:3)家系的抗病性表现推测相应F_2单株的基因型。结果显示:群体对条纹叶枯病抗性属于质量性状遗传,并且由一对主基因控制。水稻条纹叶枯病单分蘗鉴定法鉴定的结果与苗期抗性鉴定的结果也也是一致的。
利用MAPMAKER/3.0软件对F_2作图群体进行分子标记和抗病基因之间连锁分析。在所选的21对SSR引物中,有6对引物的PCR产物在宁317与武育粳3号两个亲本之间存在多态,多态性指数为28.6%。找到了与抗条纹叶枯基因连锁的SSR标记RM287。SSR标记RM287与抗病基因对遗传距离为21.7cM,位于第11染色体的一侧。
In this study,resistance to rice stripe in major japonica rice cultivar of Jiangsu Province was identified.The resistant gene was located by SSR makers.The main results were summarized as follows:
1.The mechanism of resistance to RSV and vector,SBPH was studied and analyzed in four rice varieties by using seedling inoculation,non-preference test and antibiosis test.The results indicated that the mechanism of resistance to RSV and vector SBPH was different.Huaiyoujing No.1 was moderately resistant to RSV but resistant to vector SBPH.Wuyujing No.3 was susceptible to RSV and vector SBPH. Yangjingjian7 was moderately resistant to RSV and SBPH.Zhendao88 was resistant to RSV,but moderately resistant to SBPH.IR24 was moderately resistant to RSV and SBPH
2.Crosses were made between Zhendao88、Huaiyoujing No.1、Yangjingjian7 which were resistant to RSV and Wuyujing No.3 which was susceptible to RSV.The resistance to rice stripe virus was identified by the method of the seedling inoculation. Genetical analysis indicated that the resistance to rice stripe virus in Zhendao88、Huaiyoujing No.1、yangjingjian7 were controlled by a major gene,but were not influenced by cytoplasm.
3.Reciprocal crosses were made among the four resistance cultivar:Zhendao88、Huaiyoujing No.1、yangjingjian7 and to analyze the allele inheritance model by the method of the seedling inoculation.The results indicated that the resistant gene in three cultivar were allelic.
4.In this study,a resistance cultivar Zhendao88 was crossed to a susceptible cultivar Wuyujing No.3,A F_2 segregating population composed of 200 plants was constructed from the cross between Zhendao88 and Wuyujing No.3,and each F_2 plant was selfed to obtain F_(2:3) line RSV resistance was evaluated using 200 F_(2:3) lines by the method of the separation and the seedling inoculation,and the genotype of each F_2 plant was inferred from the phenotype of corresponding F_(2:3) line.Genetic analysis indicated that the genetic model belongs to qualitative character inheritance and the resistance to rice stripe is controlled by a major gene.
SSR assay and linkage analysis were carried out to detect the resistant gene.21 SSR primers located on 11 chromosome were screened.Amplified DNA fragments were separated with 8%denaturing PAGE(polyacrylamide gelelectrophoresis) and displayed by silver staining.Four polymorphic DNA fragment were found between Zhendao88 and Wuyujing No.3.The polymorphic index is 19.0%.The microsatellite locus RM229 was found to be linked to the resistance gene.The genetic distance between RM229 and the resistant gene is 4.7cM(centMorgan).
The parent was detected with Random Amplified Polymorphic DNA.Product from PCR reaction were resolved by electrophoresis in 0.8%agarose.The positive ampliation of about 2500bp fragment generated from OPO11 was observed in Zhendao88.200 F_2 population was detected with RAPD maker OPO11.The result indicated that OPO11 was complete linkage to resistant gene.It proved that the resistant gene was stvb-i.
5.In this study,a resistance cultivar Ning317 was crossed to a susceptible cultivar Wuyujing No.3,A F_2 segregating population composed of 200 plants was constructed from the cross between Ning317 and Wuyujing No.3,and each F_2 plant was selfed to obtain F_(2:3) line RSV resistance was evaluated using 200 F_(2:3) lines by the method of the separation and the seedling inoculation and the genotype of each F_2 plant was inferred from the phenotype of corresponding F_(2:3) line.Genetic analysis indicated that the genetic model belongs to qualitative character inheritance and the resistance to rice stripe is controlled by a major gene.SSR assay and linkage analysis were carried out to detect the resistant gene.21 SSR primers located on 11 chromosome were screened.Six polymorphic DNA fragment were found between Ning317 and Wuyujing No.3.The polymorphic index is 28.6%.The microsatellite locus RM287 was found to be linked to the resistance gene.The genetic distance between RM287 and the resistant gene is 21.7cM(centMorgan).
1.选用江苏生产推广使用的粳稻品种镇稻88、淮优粳1号、扬粳鉴7号及IR24(为对照),进行抗病性和抗虫性鉴定,结果表明:淮优粳1号表现为中抗,具有非嗜性和抗生性两个抗虫特性:武育粳3号表现为感病,不具有非嗜性和抗生性两个抗虫特性;镇稻88和扬粳鉴7分别表现为抗病和中抗,具有中等非嗜性特性和抗生性特性;IR24表现为中抗,具有中等非嗜性特性和抗生性特性。
2.利用抗病品种镇稻88、淮优粳1号、扬粳鉴7与感病品种武育粳3号配制抗感杂交组合,通过室内接种鉴定,研究了对条纹叶枯的抗性遗传。遗传分析表明:镇稻88、淮优粳1号、扬粳鉴7对条纹叶枯的抗性均由1对显性核基因控制,不受细胞质遗传的影响。
3.选用镇稻88、淮优粳1号、扬粳鉴7等3个抗病品种为亲本,通过品种间进行杂交,统计F_1和F_2的发病率,来确定不同抗病品种之间的等位性。研究结果表明:镇稻88、淮优粳1号、扬粳鉴7这3个品种的抗病基因是等位的,或者是紧密连锁的。
4.利用抗病品种镇稻88与感病品种武育粳3号配制F_2群体,采用水稻条纹叶枯病单分蘗鉴定法与苗期接种鉴定的方法分别对F_2群体、F_(2:3)家系进行抗病性鉴定,并根据F_(2:3)家系的抗病性表现推测相应F_2单株的基因型。结果显示:群体对条纹叶枯病抗性属于质量性状遗传,并且由一对主基因控制。水稻条纹叶枯病单分蘗鉴定法鉴定的结果与苗期抗性鉴定的结果是一致的。
利用MAPMAKER/3.0软件对F_2作图群体进行分子标记和抗病基因之间连锁分析。在所选的21对SSR引物中,有4对引物的PCR产物在镇稻88与武育粳3号两个亲本之间存在多态,多态性指数分别为19.0%。找到了与抗条纹叶枯基因连锁的SSR标记RM229。SSR标记RM229与抗病基因对遗传距离为4.7cM,位于第11染色体的一侧。
利用RAPD标记OPO11,在亲本镇稻88、武育粳3号进行PCR扩增,用0.8%的琼脂糖进行检测,发现镇稻88中有一个特异性条带,片段大约为2500bp。用该引物对F_2分离群体的200个单株的DNA进行PCR扩增,统计结果,用MAPMAKER/EXP3.0软件计算遗传距离,结果表明RAPD标记OPO11与抗病基因的遗传距离为0cM,表现为共分离。推测此抗病基因与stvb-i是同一个位点。
5.利用抗病品种宁317与感病品种武育粳3号配制F_2群体,采用水稻条纹叶枯病单分蘗鉴定法与苗期接种鉴定的方法分别对F_2群体、F_(2:3)家系进行抗病性鉴定,并根据F_(2:3)家系的抗病性表现推测相应F_2单株的基因型。结果显示:群体对条纹叶枯病抗性属于质量性状遗传,并且由一对主基因控制。水稻条纹叶枯病单分蘗鉴定法鉴定的结果与苗期抗性鉴定的结果也也是一致的。
利用MAPMAKER/3.0软件对F_2作图群体进行分子标记和抗病基因之间连锁分析。在所选的21对SSR引物中,有6对引物的PCR产物在宁317与武育粳3号两个亲本之间存在多态,多态性指数为28.6%。找到了与抗条纹叶枯基因连锁的SSR标记RM287。SSR标记RM287与抗病基因对遗传距离为21.7cM,位于第11染色体的一侧。
In this study,resistance to rice stripe in major japonica rice cultivar of Jiangsu Province was identified.The resistant gene was located by SSR makers.The main results were summarized as follows:
1.The mechanism of resistance to RSV and vector,SBPH was studied and analyzed in four rice varieties by using seedling inoculation,non-preference test and antibiosis test.The results indicated that the mechanism of resistance to RSV and vector SBPH was different.Huaiyoujing No.1 was moderately resistant to RSV but resistant to vector SBPH.Wuyujing No.3 was susceptible to RSV and vector SBPH. Yangjingjian7 was moderately resistant to RSV and SBPH.Zhendao88 was resistant to RSV,but moderately resistant to SBPH.IR24 was moderately resistant to RSV and SBPH
2.Crosses were made between Zhendao88、Huaiyoujing No.1、Yangjingjian7 which were resistant to RSV and Wuyujing No.3 which was susceptible to RSV.The resistance to rice stripe virus was identified by the method of the seedling inoculation. Genetical analysis indicated that the resistance to rice stripe virus in Zhendao88、Huaiyoujing No.1、yangjingjian7 were controlled by a major gene,but were not influenced by cytoplasm.
3.Reciprocal crosses were made among the four resistance cultivar:Zhendao88、Huaiyoujing No.1、yangjingjian7 and to analyze the allele inheritance model by the method of the seedling inoculation.The results indicated that the resistant gene in three cultivar were allelic.
4.In this study,a resistance cultivar Zhendao88 was crossed to a susceptible cultivar Wuyujing No.3,A F_2 segregating population composed of 200 plants was constructed from the cross between Zhendao88 and Wuyujing No.3,and each F_2 plant was selfed to obtain F_(2:3) line RSV resistance was evaluated using 200 F_(2:3) lines by the method of the separation and the seedling inoculation,and the genotype of each F_2 plant was inferred from the phenotype of corresponding F_(2:3) line.Genetic analysis indicated that the genetic model belongs to qualitative character inheritance and the resistance to rice stripe is controlled by a major gene.
SSR assay and linkage analysis were carried out to detect the resistant gene.21 SSR primers located on 11 chromosome were screened.Amplified DNA fragments were separated with 8%denaturing PAGE(polyacrylamide gelelectrophoresis) and displayed by silver staining.Four polymorphic DNA fragment were found between Zhendao88 and Wuyujing No.3.The polymorphic index is 19.0%.The microsatellite locus RM229 was found to be linked to the resistance gene.The genetic distance between RM229 and the resistant gene is 4.7cM(centMorgan).
The parent was detected with Random Amplified Polymorphic DNA.Product from PCR reaction were resolved by electrophoresis in 0.8%agarose.The positive ampliation of about 2500bp fragment generated from OPO11 was observed in Zhendao88.200 F_2 population was detected with RAPD maker OPO11.The result indicated that OPO11 was complete linkage to resistant gene.It proved that the resistant gene was stvb-i.
5.In this study,a resistance cultivar Ning317 was crossed to a susceptible cultivar Wuyujing No.3,A F_2 segregating population composed of 200 plants was constructed from the cross between Ning317 and Wuyujing No.3,and each F_2 plant was selfed to obtain F_(2:3) line RSV resistance was evaluated using 200 F_(2:3) lines by the method of the separation and the seedling inoculation and the genotype of each F_2 plant was inferred from the phenotype of corresponding F_(2:3) line.Genetic analysis indicated that the genetic model belongs to qualitative character inheritance and the resistance to rice stripe is controlled by a major gene.SSR assay and linkage analysis were carried out to detect the resistant gene.21 SSR primers located on 11 chromosome were screened.Six polymorphic DNA fragment were found between Ning317 and Wuyujing No.3.The polymorphic index is 28.6%.The microsatellite locus RM287 was found to be linked to the resistance gene.The genetic distance between RM287 and the resistant gene is 21.7cM(centMorgan).
引文
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