日粮不同能量水平对绵羊屠宰性能及不同组织中HSL基因表达的影响
|
摘要
选用健康无病,4月龄体重相近的F2代杂交母羊(道赛特♂×小尾寒羊♀)36只,随机分为A组(低能量组)、B组(中能量组)、C组(高能量组)3组,每组12只。B组为对照组,按照美国NRC(1985)肉羊饲养标准中的能量需要量设计的日粮饲喂,代谢能为10.32 MJ/d,A组和C组处理是在中等能量水平基础上减少和增加30%:即低能量水平(A组)代谢能为7.21 MJ/d,高能量水平(C组)代谢能为13.48MJ/d,各处理组蛋白质及其它营养指标基本保持一致。饲喂50天后从每个试验组中随机选取6只绵羊进行屠宰,测其宰前活重、胴体重、屠宰率、背膘厚,屠宰后取两侧背最长肌(第12肋骨),后腿股二头肌测肌内脂肪含量。宰后立即取背最长肌、股二头肌、心脏及皮下脂肪组织样品,用于提取总RNA,通过优化反应条件和体系,采用实时荧光定量PCR方法对激素敏感脂肪酶(HSL)基因在背最长肌、股二头肌、心脏及皮下脂肪组织中的表达量进行了检测。同时分析了三个能量水平日粮对绵羊HSLmRNA绝对表达量的影响。试验结果如下:
1低能量组试验羊的宰前活重、胴体重、屠宰率显著低于中能量和高能量组(P<0.01或P<0.05),中能量组与高能量组的屠宰性能差异不显著(P>0.05);低能量组试验羊的背膘厚极显著低于中能量、高能量组(P<0.01),中能量组和高能量组之间差异也极显著(P<0.01)。低能量组试验羊背最长肌及股二头肌肌内脂肪含量极显著低于中能量、高能量组(P<0.01),中能量组和高能量组之间差异也极显著(P<0.01)。
2日粮的能量水平影响绵羊皮下脂肪组织、背最长肌、股二头肌和心脏HSL基因的表达,随着日粮能量水平的升高,HSLmRNA表达量减少;在皮下脂肪中三组能量水平之间的HSLmRNA绝对表达量差异极显著(P<0.01),低能量组显著高于中、高能量组的表达;在背最长肌、股二头肌中的表达低能量组与中、高能量组比较差异极显著(P<0.01),中能量与高能量组之间差异显著(P<0.05);在心脏中的表达中能量组与低、高能量组之间均差异显著(P<0.05),低能量组极显著高于高能量组(P<0.01)。
3 HSL基因在绵羊脂肪组织、背最长肌、股二头肌和心脏的表达具有组织特异性,在脂肪组织表达量最高,心脏的表达量最低,脂肪组织表达量极显著高于背最长肌、股二头肌和心脏的表达量(P<0.01),另外在肌肉组织中背最长肌的表达量高于股二头肌。
Choose healthy disease-free, 4 months old F2 generation of hybrids with similar weight crossbred ewes (Dorset sheep♂×Small Tail Han Sheep♀) ,which were randomly divided into 3 groups of 12,including group A (low-energy group), group B (middle-energy group),group C (high-energy group).As the control group,Group B was raised according to the energy requirement of American NRC(1985) Meet Sheep Feeding Standard, the energy level of metabolic energy group was 10.33 MJ / d, group A and group C based on middle-energy group on the reduction and increase of 30%,separately: that is, metabolizable energy of low energy level (group A) was 7.21 MJ / d, metabolizable energy of high energy level (group C) was 13.49 MJ / d, indicators of protein and other nutrients remained the same. After 50 days feeding, selected six sheep from each experimental group randomly and slaughtered, slaughter live weight,carcass weight,dressing percentage and backfat thickness were measured before slaughter, slaughter on both sides of longissimus muscle (12 ribs) and hind legs biceps femoris were obtained for intramuscular fat content. Longissimus, biceps femoris, heart and the subcutaneous adipose tissue samples were taken in the shortest time for extraction of total RNA. By optimizing the reaction conditions, using real time quantitative PCR method detected hormone-sensitive lipase (HSL) gene in longissimus, biceps femoris, heart and subcutaneous adipose tissue expression. The three energy levels effects on sheep HSL mRNA expression was analyzed. The results are as follows:
1. Experimental sheep of the low-energy group before the slaughter live weight, carcass weight, dressing percentage of the low-energy group is significantly lower than that in the middle-energy group and the high-energy group (P<0.01 or P<0.05). The slaughter performance between the middle- energy group and the high-energy group was not significant difference (P>0.05); the sheep's back fat thickness, longissimus muscle and biceps femoris within the fat content of intramuscular fat content of the low-energy group was significantly lower than that in the middle-energy group and the high- energy group (P<0.01), the energy difference between the middle-energy group and the high-energy group were also significantly (P<0.01).The longissimus muscle shear force of high-energy group was significantly lower than the middle-energy group and the low energy group (P <0.01); The two head muscle shear force of the high-energy group was significantly lower than the low energy group (P <0.01).
2. The expression of HSL mRNA decreased with the levels of dietary energy increased in longissimus muscle, biceps femoris, heart and subcutaneous adipose tissue;the absolute expression of HSL mRNA during the three groups in abdominal subcutaneous fat was significant difference (P<0.01), the expression in the low-energy group was significantly higher than the middle-energy group and the high-energy group expression; compared with the high-energy group, the expression in longissimus, biceps femoris of the low-energy group was significant difference (P<0.01), the energy difference between the middle-energy group and the high-energy group was significantly (P<0.05); the expression in the heart of the middle-energy group was significantly difference with the low-energy group and the high-energy group(P <0.05), and the low-energy group was significantly higher than the high-energy group (P <0.01).
3. HSL gene in sheep adipose tissue, longissimus muscle, biceps femoris and heart tissue was expressed specificly, its expression in adipose tissue was highest and the lowest in the heart. The expression of in adipose tissue was significantly higher than in the longissimus muscle, biceps femoris, and heart (P<0.01).Besides, the expression of longissimus in muscle tissue was higher than in biceps femoris.
1低能量组试验羊的宰前活重、胴体重、屠宰率显著低于中能量和高能量组(P<0.01或P<0.05),中能量组与高能量组的屠宰性能差异不显著(P>0.05);低能量组试验羊的背膘厚极显著低于中能量、高能量组(P<0.01),中能量组和高能量组之间差异也极显著(P<0.01)。低能量组试验羊背最长肌及股二头肌肌内脂肪含量极显著低于中能量、高能量组(P<0.01),中能量组和高能量组之间差异也极显著(P<0.01)。
2日粮的能量水平影响绵羊皮下脂肪组织、背最长肌、股二头肌和心脏HSL基因的表达,随着日粮能量水平的升高,HSLmRNA表达量减少;在皮下脂肪中三组能量水平之间的HSLmRNA绝对表达量差异极显著(P<0.01),低能量组显著高于中、高能量组的表达;在背最长肌、股二头肌中的表达低能量组与中、高能量组比较差异极显著(P<0.01),中能量与高能量组之间差异显著(P<0.05);在心脏中的表达中能量组与低、高能量组之间均差异显著(P<0.05),低能量组极显著高于高能量组(P<0.01)。
3 HSL基因在绵羊脂肪组织、背最长肌、股二头肌和心脏的表达具有组织特异性,在脂肪组织表达量最高,心脏的表达量最低,脂肪组织表达量极显著高于背最长肌、股二头肌和心脏的表达量(P<0.01),另外在肌肉组织中背最长肌的表达量高于股二头肌。
Choose healthy disease-free, 4 months old F2 generation of hybrids with similar weight crossbred ewes (Dorset sheep♂×Small Tail Han Sheep♀) ,which were randomly divided into 3 groups of 12,including group A (low-energy group), group B (middle-energy group),group C (high-energy group).As the control group,Group B was raised according to the energy requirement of American NRC(1985) Meet Sheep Feeding Standard, the energy level of metabolic energy group was 10.33 MJ / d, group A and group C based on middle-energy group on the reduction and increase of 30%,separately: that is, metabolizable energy of low energy level (group A) was 7.21 MJ / d, metabolizable energy of high energy level (group C) was 13.49 MJ / d, indicators of protein and other nutrients remained the same. After 50 days feeding, selected six sheep from each experimental group randomly and slaughtered, slaughter live weight,carcass weight,dressing percentage and backfat thickness were measured before slaughter, slaughter on both sides of longissimus muscle (12 ribs) and hind legs biceps femoris were obtained for intramuscular fat content. Longissimus, biceps femoris, heart and the subcutaneous adipose tissue samples were taken in the shortest time for extraction of total RNA. By optimizing the reaction conditions, using real time quantitative PCR method detected hormone-sensitive lipase (HSL) gene in longissimus, biceps femoris, heart and subcutaneous adipose tissue expression. The three energy levels effects on sheep HSL mRNA expression was analyzed. The results are as follows:
1. Experimental sheep of the low-energy group before the slaughter live weight, carcass weight, dressing percentage of the low-energy group is significantly lower than that in the middle-energy group and the high-energy group (P<0.01 or P<0.05). The slaughter performance between the middle- energy group and the high-energy group was not significant difference (P>0.05); the sheep's back fat thickness, longissimus muscle and biceps femoris within the fat content of intramuscular fat content of the low-energy group was significantly lower than that in the middle-energy group and the high- energy group (P<0.01), the energy difference between the middle-energy group and the high-energy group were also significantly (P<0.01).The longissimus muscle shear force of high-energy group was significantly lower than the middle-energy group and the low energy group (P <0.01); The two head muscle shear force of the high-energy group was significantly lower than the low energy group (P <0.01).
2. The expression of HSL mRNA decreased with the levels of dietary energy increased in longissimus muscle, biceps femoris, heart and subcutaneous adipose tissue;the absolute expression of HSL mRNA during the three groups in abdominal subcutaneous fat was significant difference (P<0.01), the expression in the low-energy group was significantly higher than the middle-energy group and the high-energy group expression; compared with the high-energy group, the expression in longissimus, biceps femoris of the low-energy group was significant difference (P<0.01), the energy difference between the middle-energy group and the high-energy group was significantly (P<0.05); the expression in the heart of the middle-energy group was significantly difference with the low-energy group and the high-energy group(P <0.05), and the low-energy group was significantly higher than the high-energy group (P <0.01).
3. HSL gene in sheep adipose tissue, longissimus muscle, biceps femoris and heart tissue was expressed specificly, its expression in adipose tissue was highest and the lowest in the heart. The expression of in adipose tissue was significantly higher than in the longissimus muscle, biceps femoris, and heart (P<0.01).Besides, the expression of longissimus in muscle tissue was higher than in biceps femoris.
引文
[1]肖西山.中国肉羊业的发展现状与对策[J].中国草食动物,2001(01):33-35.
[2]郭健.世界肉羊业现状与甘肃肉羊产业化发展对策.中国草食动物,2004(2003-2004年全国养羊生产与学术研讨会议论文集),62-64.
[3]赵有璋.二十一世纪中国农业科技展望[M].山东科学技术出版社,1993,575-582.
[4]Klaus Hofmann,张孝若等.肉和肉制品的质量概念[J].肉类工业,1994(3):20-23.
[5]贾幼陵.中国畜牧业发展概况与前景,畜牧业及畜产品加工研究进展[M].中国农业科技出版社,1996
[6]孟乐成等.九十年代世界肉品生产概况[J].肉类工业,1994(4):43-46.
[7]戴瑞形等.肉类质量研究进展[J].肉类研究,2000,2,11-13.
[8]Ford A L, Pork.in Developments in Meat science.Vol.1ed.R.A.Lawrie、Applied Science Ltd, London, 1980,P219.
[9]Sllahidi,F&Rubin,L.J.(1986).CRC Crit,Rew in Food Science&nutrition.,24,14.
[10]Stepher, Andrews. The materials of New Zealand Boer breeding center (Unpublished Presented).
[11]Herselman et al. Heat energy for growing goats and sheep grazing different Pastures in the summer. American Society of Animal Science.1997,77(5):1258-1265.
[12]Rhee K S.et al.Composition and stability of goat meat patties as affected by breed type and feeding regimen[J].Institute of Food Technologists.1997,62(5):949-953.
[13]任守文.优质山羊肉生产技术研究[J].安徽农业科学,2001,29(1):118-120.
[14]刘国亚,毛杨毅,粱茂文等.陶赛特与山西当地绵羊杂交一代产肉性能效果[J].中国养羊,1995(2):33-34.
[15]陈维德,阿凡提,李俊年等.无角道赛特和萨福克羊在新疆的杂交利用研究[J].中国草食动物,1995,3:1-3.
[16]张若孝,邵长发,王新年等.萨福克、道赛特羊杂交中国美利奴羊杂一代的产肉性能及经济效益[J].中国畜牧杂志,1995,31(2):40.
[17]曹勇,陈滔,韩保生.道赛特羊与本地羊杂交后代生产性能分析[J].中国养羊,1997,(2):8-9.
[18]赵有璋主编.肉羊高效益生产技术[M].中国农业出版社,1998.
[19]周光宏等.肉品学[M].中国农业科技出版社,北京:1999.
[20]张克英,李学伟.维生素与矿物质对猪肉品质的影响[J].当代畜禽养殖业,2003,2 (1): 39-40.
[21]Ma Shahaglal.限制饲养期日粮蛋白水平对羔羊胴体和非胴体部分消化率和随后生长补偿的影响[J].草食家畜,1993,(4):29-35.
[22]冯涛.日粮蛋白质水平对舍饲羔羊育肥性能及肉品质影响的研究[D].西北农林科技大学:2005.
[23]赖长华.硬脂酸钙对舍饲肉羊生产性能和肉品质的影响[J].中国畜牧杂志,2003,38 (1): 29-30.
[24]周韶,李长忠.饲料中添加脂肪对南江羔羊增重的影响[J].黑龙江畜牧兽医,2003,4,13-14.
[25]武雅楠.亚麻籽对羔羊生产性能、血液生化指标和肉品质的影响[D].河北:河北农业大学.2007.
[26]郑王留.中国家畜品种及其生态学特征.1985:100-111.
[27]王维春.南江黄羊的一些种质特性[J].中国养羊,1997(4):1-2.
[28]陈韬等.云南山羊肉质研究[J].畜牧与兽医,1999,31(5):14-16.
[29]陈韬等.放牧条件下云岭山羊屠宰性能及肉质[J].云南农业大学学报,1994,14(l):58-62.
[30]陈韬,彭和禄.云南三个地方山羊品种产肉性能及肉质比较[J].中国养羊,1998,18(2):35-37.
[31]陈韬,严达伟.马关无角山羊肉质特性[J].甘肃畜牧兽医,1998,28(4):3-5.
[32]陈韬,陈文.龙陵黄山羊屠宰性能及肉质研究[J].云南农业大学学报,1999,11(3):162-167.
[33]王杰等.川西北高原山谷地区藏山羊屠宰性能研究[J].西南民族学院学报,自然版,1995,21(l):33-38.
[34]林嘉等.浙江三元杂种山羊肉质理化特性的研究[J].中国养羊,1993,13(3):31-34.
[35]张红平等.波尔山羊与南江黄羊杂交一代产肉性能和肌肉品质研究[J].四川农业大学学报2000,18(1):53-57.
[36]王梦霖.雒秋江年龄和性别对陶赛特×小尾寒羊F1代羔羊屠宰性能与肉品质的影响[J].中国畜牧兽医,2009,36(2):152-155.
[37]郑灿龙.羊肉的营养价值及其品质的影响因素[J].肉类研究,2003(1):47.
[38]鲍林,红霞.影响高寒地区放牧藏羊生长发育的几个重要因素[J].青海畜牧兽医杂志, 2003,33(3):45.
[39]王杰,王永,欧阳熙,等.藏山羊研究[J].中国畜牧杂志,1993,29(1):10-13.
[40]Saini A L, Khan B U, Singly K. Growth performance of goats under three systems of feeding[J]. Ind J Anim Sci, 1988, 58: 604-609.
[41]Fluharty F L. Energy source and ionophore supplementation effects on lamb growth, Carcess characteristics, Visceal organ mass, Diet digestibility, and nitrogen metabolism [J]. Anim. Sci. 1999,77,816-823.
[42]杨龙江,戴瑞彤,陈斌.猪肌肉组织的性能及其脂肪的影响.肉类研究,2000,2: 17-19.
[43]杨红菊,乔发东,马长伟,吉.甘得迈.肌内脂肪及其在加工过程中的水解变化[J].肉类研究,2004,2:37-39.
[44]曾勇庆,孙玉民.莱芜猪肌肉组织学特性与肉质关系的研究[J].畜牧兽医学报,1998,29(6):486-492.
[45]Ellis M. The effects of genetics and nutritional factors on pork quality [J].Asian-Aus J Anim Sci,1999(12):261-270.
[46]Blaise R, Gorber J, Rouet P, et al.Testis expression of hormone- sensitive lipase is conferred by a specific promoter that contains four regions binding testieular nuclear proteins [J].Biol Chem,1999,271(14):9327-9334.
[47]Ganon A J, et al. Primary structure of the site on bovine hormone-sensittve lipase phosphoyrlaetd by cyelic AMP-dependent protein kinase [J].FEBS Lett,1998,29,229 (1): 68-72.
[48]Holm C, Kirchgessner TG, Svenson K L, et al. Hormone-sensitive lipase: sequence, expression and chromosomnal localization to 19 cent-q13.3 [J].Science,1988,241:1503-1506.
[49]Nilsson N O, Stralfors P, Fredrikson G. Hormone-sensitive lipase: control of intracellulartri- (di-) acylglyeerol and cholesteryl ester hydrolysis [J].FEBS Letters,1980,111:125-130.
[50]Langin D, Laurell H, Holst L S, et al.[J].Proc Natl Acad Sci USA, 1993, 90:4897-4901.
[51]Gu F, Harbitz I, Chowdhary B P, BosnesM,Gustavsson I. Chromosomal localizations of the hormone-sensitive lipase and insulin receptor (INSR) genes in pig .Hereditas, 1992, 17: 231-236.
[52]刘源.猪DGAT1和Ob基因多态性及其背膘厚的关系[D]山东:山东农业大学硕士学位论文. 2006.
[53]Olsson H, Tralfors P, Belfrage P.Hormonal regulation of hormone–sensitive lipase activity and mRNA levels in isolated ratadipocytes[J].FEBS Letters,1986,209:175-180.
[54]Nilsson N O, Stralfors P, Fredrikson G. Hormone-sensitive lipase: control of intracellulartri- (di-)acylglyeerol and cholesteryl ester hydrolysis[J].FEBS Letters,1980,111:125-130.
[55]Stralfors P, Belfrage P. Phosphorylation of hormone-stimulated lipase by cyclic AMP- Dependent protein kinase[J].The Journal of Biochemical Chemistry, 183, 258 (24): 15146- 15152.
[56]邹晓庭,王友明,卢建军.二氢毗吮(diludin)抗蛋鸡脂肪肝的机理[J1.中国兽医学报,2002,22(6):620.
[57]Egan J, Greenberg AS, Min-Kun Chang, et al. Mechanism of hormone-stimulated lipolysis in adipocytes:translocation of hormone-stimulated lipase to lipid storage droplet[J] Proc. Natl. Acad. sci.USA,1992,89(3):8534-8541.
[58]Steppan C M, Bhat S, Bhat S, Brown E J, Banejee R R, Wright CM. The hormone resistin links obesity to diabetes [J].Nature,200l,409:307-126.
[59]Osuga J, Ishibashis Oka T, et al. Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy,but not in obesity .Proc Natl Acad Sci,2000,97:787-792.
[60]Haemmerle G, Zimmermann R, Hayn M, et al.Hormonesensitive lipase defieiency in mice causes diglyceride accumulation in adipose tissue,musele, and testis [J]. Biol Chem, 2002, 277:4806-815.
[61]Harada K J, Shen W J, et al. Resistance to high-fat diet-induced obesity and altered expression of adipose-specific genes in HSL-deficient mice. Am J Physiol Endoerinol Metab, 2003, 285: E1182~1195.
[62]Stich V. Adipose Tissue Lipolysis and Hormone-sensitive Lipase Expression during very-Low- Caloric Diet in Obese Female Identical Twins [J].Journal of Clinical Endocrinology and Metabolism,1997,82(3):739-744.
[63]Tanaka K, Ohtani S. Expression,activity,and localization of hormone-sensitive lipase in rat mammary gland during pregnancy and lactation [J].Japanese Journal of Zootechnical Science, 1986,57(9):747-757.
[64]Maria S, PeterAmer, Bo Ahren, et al. The Expression of Hormone- Sensitive Lipase in Clonal- Cells and Rat Islets Is Induced by Long-Term ExPosure to High Glueose[J],Oct 2001,50:2225.
[65]Takahashi K, Akiba Y. Effect of Dietary Saturated Fatty Acids on Hormone-Sensitive Lipolysis in Rat AdiPocyte [J].Anim Sci and Technol,1996,67(3):305-309.
[66]Berger J J, Barnard R J. Hormone-Sensitive Lipase Defieiency in Mouse Islets Abolishes Neutral Cholesterol Ester Hydrolase Activity but Leaves Lipolysis, Acylglycerides, Fat Oxidation,and Insulin Secretion Intact [J].J Applied Physiol,1999,87(1):227-232.
[67] Yong Qiao, Zhiguo et al. HuangDevelopmental Changes of the FAS and HSL mRNA Expression and Their Effects on the Content of Intramuscular Fat in Kazak and Xinjiang Sheep. Journal of Genetics and Genomics October 2007,34(10):909-917.
[68]夏成,王哲. INS、GLN和LP对体外培养牛脂肪细胞内HSL、LP的mRNA丰度的影响[J].畜牧兽医学报,2006,37(12),1312-1318.
[69]孔凡德,吴跃明,刘建新.激素敏感脂肪酶的研究进展[J].中国畜牧志,2002,38(6):38-40.
[70]Gavino, Daniel Ménard, Johannes H. Uptake and metabolism of structured triglyceride by Caco-2 cells: reversal of essential fatty acid deficiency [J]Am J Physiol Gastrointest Liver Physiol, Oct 1998; 275: 652.
[71]Sztalryd C, Kraemer F B. Regulation of hormone-sensitive lipase during fasting [J].Amer J Physiol,1994,266(2):179-185.
[72]杨国明,赵彦.日粮中能量对大河乌猪胴体品质的影响[J].饲料科学,2006,(5):3-6.
[73]刘东,李忠,张强.不同能量水平日粮对肉仔鸡生长及屠宰性能指标的影响研究[J].家畜生态学报,2005,26(5)32-35.
[74]闫祥林.营养水平对肉牛生产性能及牛肉品质的影响[M].南京农业大学,2003.12.
[75]邱怀.现代肉牛生产及产品加工[M].西安:陕西科学技术出版社,1995.
[76]刘太宇,耿繁军.中国肉牛业现状和发展对策[J].黄牛杂志,2003,23(2):62-64.
[77]刘丽,周光宏.饲养水平、年龄及体重对牛产肉性能影响的研究[J].黄牛杂志, 2001,27 (3): 10-14.
[78]张国梁,王重阳.营养水平对不同品种杂交肉育肥的影响[J].饲料研究, 2007,(2): 48-52.
[79]刘作华,杨飞云,孔路军,等.日粮能量水平对生长育肥猪肌内脂肪含量以及脂肪酸合成酶和激素敏感脂酶mRNA表达的影响[J].畜牧兽医学报,2007,38(9): 934-941.
[80]樊银珍,周俐兵,吴义师,等.肥育猪在不同能量和蛋白质(氨基酸)水平下生产性能和胴体品质的研究[J].饲料工业,2002,(10):14-17.
[81]Talley S M, Asplund J M, Hedrick H B, et al. Effect of feeding high-oleic-acid peanuts to growing-finishing swine on resulting carcass fatty acid profile and on carcass and meat quality characteristics. J Anim Sci,1976,42:1471-1476.
[82]Hodgson R R, Davis G W, Smith G C,Savell J W, Cross H R. Relationships between pork loin palatability traits and physical characteristics of cooked chops. Journal of Animal Science, 1991, 69: 4858-4865.
[83]Wang Y H, Byrne K A, Reverter A, Harper G S, Taniguchi M, McWilliam S M, Mannen H, Oyama K, Lehnert S A. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle. Mammalian Genome, 2005,16: 201-210.
[84]杨虹译.猪肉和禽肉质量的营养调控[M].国外畜牧学-猪与禽,1998,(3):47-50.
[85]Patrici A. Eating quality of pork in Denmark[J]. Pig Farming (supplement) ,1985,(10):56-57.
[86]Bee q Gebert S, Messikommer R. Effect of dietary energy supply and fat source on the fatty acid pattern of adipose and lean tissues and lipogenesis in the pig[J]. AnimSci, 2002, 80: 1564-1574.
[87]Talley SM, Asplund JM, Hedrick HB,et al. Effect of feeding high-oleic-acid peanuts to growing-finishing swine on resulting carcass fatty acid profile and on carcass and meat quality characteristics[J]. Anim Sci,1976,42:1471-1476.
[88]廖玉英,杨家晃,何仁春,等.日粮代谢能水平对鹅生长性能、养分利用率及肉质的影响[J].粮食与饲料工业,2006,(5):38-41.
[89]Holland P M, Abramson R D, Watson R, et a.l Detection of specific polymerase chain reaction productby utilizing the 5′to 3′exonuclease activity ofThermus aquaticusDNA pol- ymerase[J]. Proc Natl Acad Sci USA, 1991, 88: 7276-7280.
[90]Deepak S, Kottapalli K, Rakwal R, et al. Real-time PCR:revolutionizing detection and expression analysis of genes[J]. CurrGenomics, 2007, 8: 234-251.
[91]朱水芳.实时荧光聚合酶链反应(PCR)检测技术[M].北京:中国计量出版社,2003.1
[92]Fernández F, Gutiérrez J, Sorlozano A, et a.l Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6 [J]. Microbiol Res,2006, 161: 158-163.
[93]李杰之,常晓彤,林坚,等.从动物组织提取高纯度总RNA方法的改进及应用[J].生物技术通讯,2002,13(5):346-348.
[94]王桂玲,黄东阳,刘戈飞.一种从动物组织中提取高质量总RNA的方法[J].生命科学研究,2003,7(3):275-278.
[95]白纪刚,吕毅,王浩华,等.实验用猪胰腺和小肠组织总RNA提取及鉴定[J].陕西医学杂志,34(10):1182-1184.
[96]侯路珍,刘发央,谢小冬.萝卜抗菌肽基因AFP的克隆及其在大肠杆菌中的诱导表达[J].甘肃农业大学学报,2004,39(3):245-248.
[97]Payton M, Pinter K. A rapid novel method for the extraction of RNA from wild-type and genetically modified kanamycin resistant mycobacteria[J]. FEMS Microbiol Lett, 1999, 180(2):141-146.
[98]Ksenija Gasic, Alvaro Hernande, Schuyler S Korban. RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction[J]. Plant Molecular Biology Reporter,2004,22:437a-437g.
[99]Higuchi R, Fockler C, et al. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions [J]. Biotech-nology, 1993, 11(9): 1026-1030.
[100]Nittre C T, Herrmann M G, Moss A A, et al. Continuous fluorescence monitoring of rapid cycle DNA amplification [J].Biotechniques,1997,22(1): 130-138.
[101]Morrison T B, Weis T T, Writter C T. Quantification of low-copy transcriptsby continuous SYBR GreenⅠmonitoring during amplification [J].Biotechniques,1998,24 (4):954-962.
[102]Harada K, Shen W J, Patel S. Resistance to high-fat diet-induced obesity and altered expression of adi-pose-specific genes in HSL-deficient mice[J].Am JPhysiol Endocrinol MeTab, 2003, 285:1182.
[103]陈美珍,郭慧敏.复合减肥茶及其减肥机理的研究[J].食品科学, 2003,(10)
[104]Sztalryd C, Komaromy M C, Kraemer F B. Overexpression of hormone-sensitive lipase prevents triglyceride accumulation in adipocytes[J]. The Journal of Clinical Investigation, 1995, 95: 2652-2661. 105]Mary P, Thompsona, Sandra T, Coopers Bryan R, Parryb. Increased expression of the mRNA for hormone-sensitive lipase in adipose tissue of cancer patients. Biochimica et biophysics acts. Molecular basis of disease,1993,1180: 236-242.
[106]Langfort J, Ploug T, Ihlemann J, Saldo M, Holm, et al. ExPression of hormone-sensitive lipase and its regulation by adrenaline in skeletal lmusele[J]. Bioehem.1999, 340:459-465.
[107]Langfort J, Ploug T, Ihlemann J, Holm C, Galbo H. Stimulation of activity by contractions in rat skeletal musele [J].Biochem. 2000, 351:207-214.
[108]Watt M J, StellingwerffT, Heigenhauser, et al. Effect of plasma adrenaline on hormone- sensitive lipase at rest and during moderate exercise in human skeletal muscle.[J]. Physiol. 2003, 550: 325-332.
[109]Watt M J, Heigenhauser G J, F.and Spriet L L. Effeets of dynamic exereise intensity on the activation of hormone-sensitive lipase in human skeletal musele.J. Physiol. 2003, 547: 301-308. 110]Hansson O, Donsmark M, Ling C, Nevsten P, Danfelter M, Andersen JL Galbo H, Holm C.Transcriptome and proteome analysis of soleus muscle of hormone-sensitive lipase-null mice.Journal of lipid research, 2005 Dec; 46(12): 2614-23.
[111]Toepstorff C, Donsmark M, Thiele M, Vistisen B, Stewart G, Vissing K, Schjerling P, Hardie D G, Galbo H, Kiens B. Sex differences in hormone-sentitive lipase expression, activity and phosphorylation in skeletal muscle at rest and during exercise. Am J Physiol Endicrinol Metab,2006, 291(5): 1106-14.
[112]陈杰. FAS和HSLmRNA在猪背最长肌的表达及其与肌内脂肪含量的关系,农业生物技术学报2004,12,(4):422-426.
[113]乔永.湖羊羔羊不同部位肌肉肌内脂肪沉积相关荃因表达的发育性变化研究[D].南京农业大学,2004.
[2]郭健.世界肉羊业现状与甘肃肉羊产业化发展对策.中国草食动物,2004(2003-2004年全国养羊生产与学术研讨会议论文集),62-64.
[3]赵有璋.二十一世纪中国农业科技展望[M].山东科学技术出版社,1993,575-582.
[4]Klaus Hofmann,张孝若等.肉和肉制品的质量概念[J].肉类工业,1994(3):20-23.
[5]贾幼陵.中国畜牧业发展概况与前景,畜牧业及畜产品加工研究进展[M].中国农业科技出版社,1996
[6]孟乐成等.九十年代世界肉品生产概况[J].肉类工业,1994(4):43-46.
[7]戴瑞形等.肉类质量研究进展[J].肉类研究,2000,2,11-13.
[8]Ford A L, Pork.in Developments in Meat science.Vol.1ed.R.A.Lawrie、Applied Science Ltd, London, 1980,P219.
[9]Sllahidi,F&Rubin,L.J.(1986).CRC Crit,Rew in Food Science&nutrition.,24,14.
[10]Stepher, Andrews. The materials of New Zealand Boer breeding center (Unpublished Presented).
[11]Herselman et al. Heat energy for growing goats and sheep grazing different Pastures in the summer. American Society of Animal Science.1997,77(5):1258-1265.
[12]Rhee K S.et al.Composition and stability of goat meat patties as affected by breed type and feeding regimen[J].Institute of Food Technologists.1997,62(5):949-953.
[13]任守文.优质山羊肉生产技术研究[J].安徽农业科学,2001,29(1):118-120.
[14]刘国亚,毛杨毅,粱茂文等.陶赛特与山西当地绵羊杂交一代产肉性能效果[J].中国养羊,1995(2):33-34.
[15]陈维德,阿凡提,李俊年等.无角道赛特和萨福克羊在新疆的杂交利用研究[J].中国草食动物,1995,3:1-3.
[16]张若孝,邵长发,王新年等.萨福克、道赛特羊杂交中国美利奴羊杂一代的产肉性能及经济效益[J].中国畜牧杂志,1995,31(2):40.
[17]曹勇,陈滔,韩保生.道赛特羊与本地羊杂交后代生产性能分析[J].中国养羊,1997,(2):8-9.
[18]赵有璋主编.肉羊高效益生产技术[M].中国农业出版社,1998.
[19]周光宏等.肉品学[M].中国农业科技出版社,北京:1999.
[20]张克英,李学伟.维生素与矿物质对猪肉品质的影响[J].当代畜禽养殖业,2003,2 (1): 39-40.
[21]Ma Shahaglal.限制饲养期日粮蛋白水平对羔羊胴体和非胴体部分消化率和随后生长补偿的影响[J].草食家畜,1993,(4):29-35.
[22]冯涛.日粮蛋白质水平对舍饲羔羊育肥性能及肉品质影响的研究[D].西北农林科技大学:2005.
[23]赖长华.硬脂酸钙对舍饲肉羊生产性能和肉品质的影响[J].中国畜牧杂志,2003,38 (1): 29-30.
[24]周韶,李长忠.饲料中添加脂肪对南江羔羊增重的影响[J].黑龙江畜牧兽医,2003,4,13-14.
[25]武雅楠.亚麻籽对羔羊生产性能、血液生化指标和肉品质的影响[D].河北:河北农业大学.2007.
[26]郑王留.中国家畜品种及其生态学特征.1985:100-111.
[27]王维春.南江黄羊的一些种质特性[J].中国养羊,1997(4):1-2.
[28]陈韬等.云南山羊肉质研究[J].畜牧与兽医,1999,31(5):14-16.
[29]陈韬等.放牧条件下云岭山羊屠宰性能及肉质[J].云南农业大学学报,1994,14(l):58-62.
[30]陈韬,彭和禄.云南三个地方山羊品种产肉性能及肉质比较[J].中国养羊,1998,18(2):35-37.
[31]陈韬,严达伟.马关无角山羊肉质特性[J].甘肃畜牧兽医,1998,28(4):3-5.
[32]陈韬,陈文.龙陵黄山羊屠宰性能及肉质研究[J].云南农业大学学报,1999,11(3):162-167.
[33]王杰等.川西北高原山谷地区藏山羊屠宰性能研究[J].西南民族学院学报,自然版,1995,21(l):33-38.
[34]林嘉等.浙江三元杂种山羊肉质理化特性的研究[J].中国养羊,1993,13(3):31-34.
[35]张红平等.波尔山羊与南江黄羊杂交一代产肉性能和肌肉品质研究[J].四川农业大学学报2000,18(1):53-57.
[36]王梦霖.雒秋江年龄和性别对陶赛特×小尾寒羊F1代羔羊屠宰性能与肉品质的影响[J].中国畜牧兽医,2009,36(2):152-155.
[37]郑灿龙.羊肉的营养价值及其品质的影响因素[J].肉类研究,2003(1):47.
[38]鲍林,红霞.影响高寒地区放牧藏羊生长发育的几个重要因素[J].青海畜牧兽医杂志, 2003,33(3):45.
[39]王杰,王永,欧阳熙,等.藏山羊研究[J].中国畜牧杂志,1993,29(1):10-13.
[40]Saini A L, Khan B U, Singly K. Growth performance of goats under three systems of feeding[J]. Ind J Anim Sci, 1988, 58: 604-609.
[41]Fluharty F L. Energy source and ionophore supplementation effects on lamb growth, Carcess characteristics, Visceal organ mass, Diet digestibility, and nitrogen metabolism [J]. Anim. Sci. 1999,77,816-823.
[42]杨龙江,戴瑞彤,陈斌.猪肌肉组织的性能及其脂肪的影响.肉类研究,2000,2: 17-19.
[43]杨红菊,乔发东,马长伟,吉.甘得迈.肌内脂肪及其在加工过程中的水解变化[J].肉类研究,2004,2:37-39.
[44]曾勇庆,孙玉民.莱芜猪肌肉组织学特性与肉质关系的研究[J].畜牧兽医学报,1998,29(6):486-492.
[45]Ellis M. The effects of genetics and nutritional factors on pork quality [J].Asian-Aus J Anim Sci,1999(12):261-270.
[46]Blaise R, Gorber J, Rouet P, et al.Testis expression of hormone- sensitive lipase is conferred by a specific promoter that contains four regions binding testieular nuclear proteins [J].Biol Chem,1999,271(14):9327-9334.
[47]Ganon A J, et al. Primary structure of the site on bovine hormone-sensittve lipase phosphoyrlaetd by cyelic AMP-dependent protein kinase [J].FEBS Lett,1998,29,229 (1): 68-72.
[48]Holm C, Kirchgessner TG, Svenson K L, et al. Hormone-sensitive lipase: sequence, expression and chromosomnal localization to 19 cent-q13.3 [J].Science,1988,241:1503-1506.
[49]Nilsson N O, Stralfors P, Fredrikson G. Hormone-sensitive lipase: control of intracellulartri- (di-) acylglyeerol and cholesteryl ester hydrolysis [J].FEBS Letters,1980,111:125-130.
[50]Langin D, Laurell H, Holst L S, et al.[J].Proc Natl Acad Sci USA, 1993, 90:4897-4901.
[51]Gu F, Harbitz I, Chowdhary B P, BosnesM,Gustavsson I. Chromosomal localizations of the hormone-sensitive lipase and insulin receptor (INSR) genes in pig .Hereditas, 1992, 17: 231-236.
[52]刘源.猪DGAT1和Ob基因多态性及其背膘厚的关系[D]山东:山东农业大学硕士学位论文. 2006.
[53]Olsson H, Tralfors P, Belfrage P.Hormonal regulation of hormone–sensitive lipase activity and mRNA levels in isolated ratadipocytes[J].FEBS Letters,1986,209:175-180.
[54]Nilsson N O, Stralfors P, Fredrikson G. Hormone-sensitive lipase: control of intracellulartri- (di-)acylglyeerol and cholesteryl ester hydrolysis[J].FEBS Letters,1980,111:125-130.
[55]Stralfors P, Belfrage P. Phosphorylation of hormone-stimulated lipase by cyclic AMP- Dependent protein kinase[J].The Journal of Biochemical Chemistry, 183, 258 (24): 15146- 15152.
[56]邹晓庭,王友明,卢建军.二氢毗吮(diludin)抗蛋鸡脂肪肝的机理[J1.中国兽医学报,2002,22(6):620.
[57]Egan J, Greenberg AS, Min-Kun Chang, et al. Mechanism of hormone-stimulated lipolysis in adipocytes:translocation of hormone-stimulated lipase to lipid storage droplet[J] Proc. Natl. Acad. sci.USA,1992,89(3):8534-8541.
[58]Steppan C M, Bhat S, Bhat S, Brown E J, Banejee R R, Wright CM. The hormone resistin links obesity to diabetes [J].Nature,200l,409:307-126.
[59]Osuga J, Ishibashis Oka T, et al. Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy,but not in obesity .Proc Natl Acad Sci,2000,97:787-792.
[60]Haemmerle G, Zimmermann R, Hayn M, et al.Hormonesensitive lipase defieiency in mice causes diglyceride accumulation in adipose tissue,musele, and testis [J]. Biol Chem, 2002, 277:4806-815.
[61]Harada K J, Shen W J, et al. Resistance to high-fat diet-induced obesity and altered expression of adipose-specific genes in HSL-deficient mice. Am J Physiol Endoerinol Metab, 2003, 285: E1182~1195.
[62]Stich V. Adipose Tissue Lipolysis and Hormone-sensitive Lipase Expression during very-Low- Caloric Diet in Obese Female Identical Twins [J].Journal of Clinical Endocrinology and Metabolism,1997,82(3):739-744.
[63]Tanaka K, Ohtani S. Expression,activity,and localization of hormone-sensitive lipase in rat mammary gland during pregnancy and lactation [J].Japanese Journal of Zootechnical Science, 1986,57(9):747-757.
[64]Maria S, PeterAmer, Bo Ahren, et al. The Expression of Hormone- Sensitive Lipase in Clonal- Cells and Rat Islets Is Induced by Long-Term ExPosure to High Glueose[J],Oct 2001,50:2225.
[65]Takahashi K, Akiba Y. Effect of Dietary Saturated Fatty Acids on Hormone-Sensitive Lipolysis in Rat AdiPocyte [J].Anim Sci and Technol,1996,67(3):305-309.
[66]Berger J J, Barnard R J. Hormone-Sensitive Lipase Defieiency in Mouse Islets Abolishes Neutral Cholesterol Ester Hydrolase Activity but Leaves Lipolysis, Acylglycerides, Fat Oxidation,and Insulin Secretion Intact [J].J Applied Physiol,1999,87(1):227-232.
[67] Yong Qiao, Zhiguo et al. HuangDevelopmental Changes of the FAS and HSL mRNA Expression and Their Effects on the Content of Intramuscular Fat in Kazak and Xinjiang Sheep. Journal of Genetics and Genomics October 2007,34(10):909-917.
[68]夏成,王哲. INS、GLN和LP对体外培养牛脂肪细胞内HSL、LP的mRNA丰度的影响[J].畜牧兽医学报,2006,37(12),1312-1318.
[69]孔凡德,吴跃明,刘建新.激素敏感脂肪酶的研究进展[J].中国畜牧志,2002,38(6):38-40.
[70]Gavino, Daniel Ménard, Johannes H. Uptake and metabolism of structured triglyceride by Caco-2 cells: reversal of essential fatty acid deficiency [J]Am J Physiol Gastrointest Liver Physiol, Oct 1998; 275: 652.
[71]Sztalryd C, Kraemer F B. Regulation of hormone-sensitive lipase during fasting [J].Amer J Physiol,1994,266(2):179-185.
[72]杨国明,赵彦.日粮中能量对大河乌猪胴体品质的影响[J].饲料科学,2006,(5):3-6.
[73]刘东,李忠,张强.不同能量水平日粮对肉仔鸡生长及屠宰性能指标的影响研究[J].家畜生态学报,2005,26(5)32-35.
[74]闫祥林.营养水平对肉牛生产性能及牛肉品质的影响[M].南京农业大学,2003.12.
[75]邱怀.现代肉牛生产及产品加工[M].西安:陕西科学技术出版社,1995.
[76]刘太宇,耿繁军.中国肉牛业现状和发展对策[J].黄牛杂志,2003,23(2):62-64.
[77]刘丽,周光宏.饲养水平、年龄及体重对牛产肉性能影响的研究[J].黄牛杂志, 2001,27 (3): 10-14.
[78]张国梁,王重阳.营养水平对不同品种杂交肉育肥的影响[J].饲料研究, 2007,(2): 48-52.
[79]刘作华,杨飞云,孔路军,等.日粮能量水平对生长育肥猪肌内脂肪含量以及脂肪酸合成酶和激素敏感脂酶mRNA表达的影响[J].畜牧兽医学报,2007,38(9): 934-941.
[80]樊银珍,周俐兵,吴义师,等.肥育猪在不同能量和蛋白质(氨基酸)水平下生产性能和胴体品质的研究[J].饲料工业,2002,(10):14-17.
[81]Talley S M, Asplund J M, Hedrick H B, et al. Effect of feeding high-oleic-acid peanuts to growing-finishing swine on resulting carcass fatty acid profile and on carcass and meat quality characteristics. J Anim Sci,1976,42:1471-1476.
[82]Hodgson R R, Davis G W, Smith G C,Savell J W, Cross H R. Relationships between pork loin palatability traits and physical characteristics of cooked chops. Journal of Animal Science, 1991, 69: 4858-4865.
[83]Wang Y H, Byrne K A, Reverter A, Harper G S, Taniguchi M, McWilliam S M, Mannen H, Oyama K, Lehnert S A. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle. Mammalian Genome, 2005,16: 201-210.
[84]杨虹译.猪肉和禽肉质量的营养调控[M].国外畜牧学-猪与禽,1998,(3):47-50.
[85]Patrici A. Eating quality of pork in Denmark[J]. Pig Farming (supplement) ,1985,(10):56-57.
[86]Bee q Gebert S, Messikommer R. Effect of dietary energy supply and fat source on the fatty acid pattern of adipose and lean tissues and lipogenesis in the pig[J]. AnimSci, 2002, 80: 1564-1574.
[87]Talley SM, Asplund JM, Hedrick HB,et al. Effect of feeding high-oleic-acid peanuts to growing-finishing swine on resulting carcass fatty acid profile and on carcass and meat quality characteristics[J]. Anim Sci,1976,42:1471-1476.
[88]廖玉英,杨家晃,何仁春,等.日粮代谢能水平对鹅生长性能、养分利用率及肉质的影响[J].粮食与饲料工业,2006,(5):38-41.
[89]Holland P M, Abramson R D, Watson R, et a.l Detection of specific polymerase chain reaction productby utilizing the 5′to 3′exonuclease activity ofThermus aquaticusDNA pol- ymerase[J]. Proc Natl Acad Sci USA, 1991, 88: 7276-7280.
[90]Deepak S, Kottapalli K, Rakwal R, et al. Real-time PCR:revolutionizing detection and expression analysis of genes[J]. CurrGenomics, 2007, 8: 234-251.
[91]朱水芳.实时荧光聚合酶链反应(PCR)检测技术[M].北京:中国计量出版社,2003.1
[92]Fernández F, Gutiérrez J, Sorlozano A, et a.l Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6 [J]. Microbiol Res,2006, 161: 158-163.
[93]李杰之,常晓彤,林坚,等.从动物组织提取高纯度总RNA方法的改进及应用[J].生物技术通讯,2002,13(5):346-348.
[94]王桂玲,黄东阳,刘戈飞.一种从动物组织中提取高质量总RNA的方法[J].生命科学研究,2003,7(3):275-278.
[95]白纪刚,吕毅,王浩华,等.实验用猪胰腺和小肠组织总RNA提取及鉴定[J].陕西医学杂志,34(10):1182-1184.
[96]侯路珍,刘发央,谢小冬.萝卜抗菌肽基因AFP的克隆及其在大肠杆菌中的诱导表达[J].甘肃农业大学学报,2004,39(3):245-248.
[97]Payton M, Pinter K. A rapid novel method for the extraction of RNA from wild-type and genetically modified kanamycin resistant mycobacteria[J]. FEMS Microbiol Lett, 1999, 180(2):141-146.
[98]Ksenija Gasic, Alvaro Hernande, Schuyler S Korban. RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction[J]. Plant Molecular Biology Reporter,2004,22:437a-437g.
[99]Higuchi R, Fockler C, et al. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions [J]. Biotech-nology, 1993, 11(9): 1026-1030.
[100]Nittre C T, Herrmann M G, Moss A A, et al. Continuous fluorescence monitoring of rapid cycle DNA amplification [J].Biotechniques,1997,22(1): 130-138.
[101]Morrison T B, Weis T T, Writter C T. Quantification of low-copy transcriptsby continuous SYBR GreenⅠmonitoring during amplification [J].Biotechniques,1998,24 (4):954-962.
[102]Harada K, Shen W J, Patel S. Resistance to high-fat diet-induced obesity and altered expression of adi-pose-specific genes in HSL-deficient mice[J].Am JPhysiol Endocrinol MeTab, 2003, 285:1182.
[103]陈美珍,郭慧敏.复合减肥茶及其减肥机理的研究[J].食品科学, 2003,(10)
[104]Sztalryd C, Komaromy M C, Kraemer F B. Overexpression of hormone-sensitive lipase prevents triglyceride accumulation in adipocytes[J]. The Journal of Clinical Investigation, 1995, 95: 2652-2661. 105]Mary P, Thompsona, Sandra T, Coopers Bryan R, Parryb. Increased expression of the mRNA for hormone-sensitive lipase in adipose tissue of cancer patients. Biochimica et biophysics acts. Molecular basis of disease,1993,1180: 236-242.
[106]Langfort J, Ploug T, Ihlemann J, Saldo M, Holm, et al. ExPression of hormone-sensitive lipase and its regulation by adrenaline in skeletal lmusele[J]. Bioehem.1999, 340:459-465.
[107]Langfort J, Ploug T, Ihlemann J, Holm C, Galbo H. Stimulation of activity by contractions in rat skeletal musele [J].Biochem. 2000, 351:207-214.
[108]Watt M J, StellingwerffT, Heigenhauser, et al. Effect of plasma adrenaline on hormone- sensitive lipase at rest and during moderate exercise in human skeletal muscle.[J]. Physiol. 2003, 550: 325-332.
[109]Watt M J, Heigenhauser G J, F.and Spriet L L. Effeets of dynamic exereise intensity on the activation of hormone-sensitive lipase in human skeletal musele.J. Physiol. 2003, 547: 301-308. 110]Hansson O, Donsmark M, Ling C, Nevsten P, Danfelter M, Andersen JL Galbo H, Holm C.Transcriptome and proteome analysis of soleus muscle of hormone-sensitive lipase-null mice.Journal of lipid research, 2005 Dec; 46(12): 2614-23.
[111]Toepstorff C, Donsmark M, Thiele M, Vistisen B, Stewart G, Vissing K, Schjerling P, Hardie D G, Galbo H, Kiens B. Sex differences in hormone-sentitive lipase expression, activity and phosphorylation in skeletal muscle at rest and during exercise. Am J Physiol Endicrinol Metab,2006, 291(5): 1106-14.
[112]陈杰. FAS和HSLmRNA在猪背最长肌的表达及其与肌内脂肪含量的关系,农业生物技术学报2004,12,(4):422-426.
[113]乔永.湖羊羔羊不同部位肌肉肌内脂肪沉积相关荃因表达的发育性变化研究[D].南京农业大学,2004.