PAS-Na对锰中毒大鼠学习记忆功能障碍及海马结构GFAP表达的影响
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摘要
目的:探讨PAS-Na对染锰大鼠学习记忆障碍及海马结构胶质原纤维酸性蛋白(GFAP)阳性细胞表达的影响。
方法:雄性SD大鼠144只在1~12周时随机分为空白组36只、染锰组72只﹑PAS-Na预防组36只。染锰组腹腔注射(ip)氯化锰(MnCl2·4H2O 15mg/kg),空白组腹腔注射等容量生理盐水,每日1次,每周5天,连续12周。PAS-Na预防组在每周的1、3、5天染锰结束后再背部皮下注射(sc)PAS-Na 200mg/kg;在染锰12周结束后把染锰组再随机分为染锰对照组(染锰组),PAS-Na治疗组,PAS-Na治疗组给予背部皮下注射PAS-Na 200mg/kg,染锰组和空白组给予背部皮下注射等容量的生理盐水,每日1次,每周3天,共6周。采用Morris水迷宫检测大鼠学习记忆能力,免疫组织化学方法检测海马CA1区和齿状回GFAP阳性细胞数。
结果:染锰末周大鼠体重增加趋势明显减低,PAS-Na治疗使染锰大鼠体重增加,差异有统计学意义(P<0.05)。在第18周染锰组逃避潜伏期比空白组延长,但(P﹥0.05),PAS-Na治疗组在实验的1、2天游泳总路程比染锰组缩短(P<0.05)。在第6周、12周及18周染锰组GFAP阳性细胞数比空白组多(P <0.05～0.01),在12周PAS-Na预防组GFAP阳性细胞数较染锰组减少(P<0.05),但仍较空白组多。
结论:锰暴露使大鼠体重增加趋势减缓和学习记忆能力下降,海马胶质原纤维酸性蛋白(GFAP)表达增加,PAS-Na对锰致大鼠生长发育迟缓,学习记忆障碍和海马胶质细胞异常增生可能有干预作用。
Objective: To explore the effects of sodium para-aminosalicylic acid (PAS-Na) on learning memory and expression of glial fibrillary acidic protein (GFAP) positive cells in hippocampus of subacute manganese(Mn)-exposed rats.
Methods:144 male Sprague-Dawley rats were randomly divided into the control, Mn-exposed, PAS-Na prevention group. The Mn-exposed rats received daily intraperitoneally injections (i.p.) of manganese chloride (MnCl2·4H2O 15mg/kg), one time a day , 5 days/week for 12 weeks, The control group were injected with the same Capacity saline。PAS-Na prevention group in the same time exposed to manganese 1,3,5 days a week subcutaneous injection (sc) PAS-Na 200mg/kg, Manganese in the dye after the end of 12 weeks of Mn group was randomly divided into control group exposed to manganese (Mn in group), PAS-Na treatment group,The treatment group after 12 weeks of Mn given subcutaneous injection of PAS-Na 200mg/kg, Exposed to manganese group and control group were given subcutaneous injection of the same volume of saline, 1 day, 3 days per week for 6 weeks.The ability of learning memory was tested by Morris Water Maze (MWM) and the GFAP-positive cells in the hippocampal CA1 area and dentate gyrus was detected by immunohis to chemical technique.
Results:The weight gain of Mn-exposed group was evidently reduced in 12 weeks and still lasted 18 weeks after suspending Mn exposure (P<0.05). In the 18 week,The escape latency of Mn-exposed group were longer than those of the control group but(P﹥0.05), In the 1,2 days ,The total swimming distance of PAS-Na treatment group were shorter than those of the Mn-exposed group(P<0.05).In the 6 week、12 week and 18 week GFAP positive cells in the manganese-exposed group increased compared with that of control group(P <0.05～0.01),In the 12 week ,GFAP positive cells in the manganese-exposed group decreased compared with that of manganese-exposed group,But still more than the control group。
Conclusions : PAS-Na may have the intervention effect on growth retardation,learning and memory impairment, glial abnormal proliferation in hippocampus of rats induced by manganese.
方法:雄性SD大鼠144只在1~12周时随机分为空白组36只、染锰组72只﹑PAS-Na预防组36只。染锰组腹腔注射(ip)氯化锰(MnCl2·4H2O 15mg/kg),空白组腹腔注射等容量生理盐水,每日1次,每周5天,连续12周。PAS-Na预防组在每周的1、3、5天染锰结束后再背部皮下注射(sc)PAS-Na 200mg/kg;在染锰12周结束后把染锰组再随机分为染锰对照组(染锰组),PAS-Na治疗组,PAS-Na治疗组给予背部皮下注射PAS-Na 200mg/kg,染锰组和空白组给予背部皮下注射等容量的生理盐水,每日1次,每周3天,共6周。采用Morris水迷宫检测大鼠学习记忆能力,免疫组织化学方法检测海马CA1区和齿状回GFAP阳性细胞数。
结果:染锰末周大鼠体重增加趋势明显减低,PAS-Na治疗使染锰大鼠体重增加,差异有统计学意义(P<0.05)。在第18周染锰组逃避潜伏期比空白组延长,但(P﹥0.05),PAS-Na治疗组在实验的1、2天游泳总路程比染锰组缩短(P<0.05)。在第6周、12周及18周染锰组GFAP阳性细胞数比空白组多(P <0.05～0.01),在12周PAS-Na预防组GFAP阳性细胞数较染锰组减少(P<0.05),但仍较空白组多。
结论:锰暴露使大鼠体重增加趋势减缓和学习记忆能力下降,海马胶质原纤维酸性蛋白(GFAP)表达增加,PAS-Na对锰致大鼠生长发育迟缓,学习记忆障碍和海马胶质细胞异常增生可能有干预作用。
Objective: To explore the effects of sodium para-aminosalicylic acid (PAS-Na) on learning memory and expression of glial fibrillary acidic protein (GFAP) positive cells in hippocampus of subacute manganese(Mn)-exposed rats.
Methods:144 male Sprague-Dawley rats were randomly divided into the control, Mn-exposed, PAS-Na prevention group. The Mn-exposed rats received daily intraperitoneally injections (i.p.) of manganese chloride (MnCl2·4H2O 15mg/kg), one time a day , 5 days/week for 12 weeks, The control group were injected with the same Capacity saline。PAS-Na prevention group in the same time exposed to manganese 1,3,5 days a week subcutaneous injection (sc) PAS-Na 200mg/kg, Manganese in the dye after the end of 12 weeks of Mn group was randomly divided into control group exposed to manganese (Mn in group), PAS-Na treatment group,The treatment group after 12 weeks of Mn given subcutaneous injection of PAS-Na 200mg/kg, Exposed to manganese group and control group were given subcutaneous injection of the same volume of saline, 1 day, 3 days per week for 6 weeks.The ability of learning memory was tested by Morris Water Maze (MWM) and the GFAP-positive cells in the hippocampal CA1 area and dentate gyrus was detected by immunohis to chemical technique.
Results:The weight gain of Mn-exposed group was evidently reduced in 12 weeks and still lasted 18 weeks after suspending Mn exposure (P<0.05). In the 18 week,The escape latency of Mn-exposed group were longer than those of the control group but(P﹥0.05), In the 1,2 days ,The total swimming distance of PAS-Na treatment group were shorter than those of the Mn-exposed group(P<0.05).In the 6 week、12 week and 18 week GFAP positive cells in the manganese-exposed group increased compared with that of control group(P <0.05～0.01),In the 12 week ,GFAP positive cells in the manganese-exposed group decreased compared with that of manganese-exposed group,But still more than the control group。
Conclusions : PAS-Na may have the intervention effect on growth retardation,learning and memory impairment, glial abnormal proliferation in hippocampus of rats induced by manganese.
引文
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