蝙蝠葛酚性碱对实验性心肌缺血保护作用的研究
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摘要
心肌缺血是一种常见的心脏病理生理改变，许多因素都可导致心肌缺血，而发病率极高的冠心病更是导致心肌缺血的主要原因。本文从中西医两个角度对心肌缺血研究现状作了阐述。
本实验借助生物化学、病理学以及生理学研究手段，观察了蝙蝠葛酚性碱对结扎大鼠左冠状动脉前降支所造成的急性心肌缺血模型的影响，并通过Ⅱ导心电图改变、血清中超氧化物歧化酶(SOD)活性、血清中丙二醛(MDA)含量和血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)的活性，以及心肌组织形态学改变，探讨了蝙蝠葛酚性碱对大鼠心肌缺血损伤的影响及其作用机制。
实验结果表明，蝙蝠葛酚性碱给药组大鼠血清中SOD活性显著升高；血清中MDA含量显著降低；血清中CK、LDH活性显著降低，并可抑制ST段抬高幅度。与复方丹参片相比，蝙蝠葛酚性碱显示出更明显的抗心肌缺血作用。
Myocardial ischemia is a common of pathopysiology change of ischemic heart disease,many factors cause it,coronary heart disease is the most important factor.The thesis summarize present situation study about myocardial ischemia from modern medicine and traditional Chinese medicine.
The experiment observed the effect of phenolic alkaloids from menispermum dauricum(PAMD) on the acute myocardial ischemia in rats by the means of biochemistry > pathology -, and physiology .The model was established by occluding left anterior descending coronary artery(LAD). The change of II ?write electrocardiogram (ECG) ? Superoxide dismutase(SOD) activity> Malondial dehyde(MDA) content^ Creatine kinase(CK) activity -, Lactic dehydrogenase(LDH) acyivity in serum were determined the results indicated that after administration of PAMD.
The results showed that SOD activity in serum was significantly higher MDA content was lower ^ CK activity was lower in PAMD group than those of model group. PAMD can also inhibit ST條evel raising range .PAMD showed more significant protective effect on myocardial of ischemia compared to the tablet of compound salvia miltiorrhiza Bge of positive control group.
本实验借助生物化学、病理学以及生理学研究手段，观察了蝙蝠葛酚性碱对结扎大鼠左冠状动脉前降支所造成的急性心肌缺血模型的影响，并通过Ⅱ导心电图改变、血清中超氧化物歧化酶(SOD)活性、血清中丙二醛(MDA)含量和血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)的活性，以及心肌组织形态学改变，探讨了蝙蝠葛酚性碱对大鼠心肌缺血损伤的影响及其作用机制。
实验结果表明，蝙蝠葛酚性碱给药组大鼠血清中SOD活性显著升高；血清中MDA含量显著降低；血清中CK、LDH活性显著降低，并可抑制ST段抬高幅度。与复方丹参片相比，蝙蝠葛酚性碱显示出更明显的抗心肌缺血作用。
Myocardial ischemia is a common of pathopysiology change of ischemic heart disease,many factors cause it,coronary heart disease is the most important factor.The thesis summarize present situation study about myocardial ischemia from modern medicine and traditional Chinese medicine.
The experiment observed the effect of phenolic alkaloids from menispermum dauricum(PAMD) on the acute myocardial ischemia in rats by the means of biochemistry > pathology -, and physiology .The model was established by occluding left anterior descending coronary artery(LAD). The change of II ?write electrocardiogram (ECG) ? Superoxide dismutase(SOD) activity> Malondial dehyde(MDA) content^ Creatine kinase(CK) activity -, Lactic dehydrogenase(LDH) acyivity in serum were determined the results indicated that after administration of PAMD.
The results showed that SOD activity in serum was significantly higher MDA content was lower ^ CK activity was lower in PAMD group than those of model group. PAMD can also inhibit ST條evel raising range .PAMD showed more significant protective effect on myocardial of ischemia compared to the tablet of compound salvia miltiorrhiza Bge of positive control group.
引文
[1]陈灏珠 实用内科学 人民卫生出版社
[2]高血压与冠心病 全国心血管防治研究办公室编 科学出版社
[3]陈纪林 中华心血管病杂志 1997、25(1)70～71
[4]Reimer KA et al, Four brief Periiods of myocardial ischemic cause no cumulative ATP loss or necrosia Am J physio、1986, 251:H1306
[5]Murry CE, Jennings RB, Reimer KA, et al、preconditioning with ischemia: adelay of lethal cell injury ischemic myocardium circulation, 1986、74(5)1124
[6]Murry CE et al. Ischemic preconditioning slows energy metabolism and delays ultrastructural damage dauring a sustaind ischemic episode circ Res, 1990,66:913
[7]Marber MS et al. circulation 1993; 88: 1264～1272
[8]Baxter CF et al. circulation. 1995;92(suppl I)I--389
[9]Thornton JD et al. Intravenous pretreatment with A_1 sclective alenosine analogues protects the heart against infarction circulatin、1992,85:659
[10]Van wylen PGL Effect of ischmic preconditioning on interestial purime metabolite and loctate a ccumulation dauring myocardial ischemic circulatior 1994,89(5)2238
[11]Liu Y, Downey JM preconditioning against infarction in the rat heart does not involve pertussis toxin sensitive G, protein cardiovasc Res, 1993,27:68
[12]Klaus v, Kan Y, Keijo Pet al. mechanisms of ischemic proconditioning in rat myocardium、circulation 1995,91(5):2810
[13]陈莹莹等 心血管病学进展 1997、18(5)271～276
[14]Baner jeea, Locke winter, Rogers KB, et al, preconditioning against myocardial dysfunction after ischemia and reperfusion by an alpna 1-adrenergic mechanism cir Res, 1993,73(4)656
[15]Noda k, sasayui M, et al, Role of locally formed angiotensinⅡand
bredykinin in the reduction of myocardial infarct size in dogs,cardiovasc Res 1993,27 (2) :334
[16] Liu Y,Tauchida A,cohen Mv et al、 pretreatment with angiotensi II activates protein kinasec and limits myocardial infarction in isclated rabbit hearts,J Mol cell cardiol,1995,27 (3) 883
[17] Richard v,Blauc T,Kaeffer N et al、 myocardial and cornary end othelial protective effects of occetylchdine after myocardial ischemia and reperfusion in rats:role of nitric oxide Br、 J Pharmacol,1995,115(8) 1532
[18] 赵丽娟 中国乡村医生杂志 1994、 7, 16-19
[19] Southorn PA, mayo clin proc,1998,63:381
[20] Thompson JA,Hess ML、 the oxygen free radical system:a fundamental mechanism in the production of myocardial necrosis, prog cardiovasc Dis,1986;26(6) :449
[21] She AC、 Jennings RB,kinetics of calcium accumlation in acute myocardial ischemic injury[J]、 AMJ Rathol,1972,67:441-447
[22] Ikeda u,Arisaka H,Takeda K, et al、 protein kinase catetivation aggrevate hyposic myocardial injury by stimulating Na+/H+ exchange[J]、 Mol cell cardiol 1988,20:493-498
[23] Xu X, star RA, Tortorici G, et al、 Depletion of interacellular Ca2+store activantes nitricoxide synthase to generate cGMP and regulate Ca2+ influx [J].J Biol chem 1994,269:12645-2653
[24] Brauwald E, Kloner R、 the stunned myocardium:prolonged,postischemic ventricular dysfunction、 circulation,1982,6,1146
[25] 唐强 心血管病学进展 2002,21(1) 15-18
[26] Rahimthoola SH, the hibernating myocardium. Am Heart J 1989,17:211
[27] Boden WE,Brooks WW conrad CH, et al,Incomplete delaved functional recovery late after reperfusion following acute myocardial infaraction " myocardium" AM Heart J,1995,130:922
[28] Ferrari R,La canna G,Ginbbini R,et al,left ventricnlar dysfunction due to
stunning and hibernation in patients cadiovas Drugs Ther, 1994,8:371
[29]Sabia P J、poers ER、Ragosta M,et al,Am associated between collateral blood folow and myocardial viability in patients with recent myocardial infarction、N Engl J Med,1992,327:1825
[30]杨维益等 浙江中医杂志，1981；(9)：386
[31]姚真敏等 新中医，1986；(4)：13
[32]闫洪臣等 陕西新医药，1979；(6)：49
[33]宋崇顺等 辽宁中医杂志，1987；(5)：42
[34]翁维良等 中医杂志，1989；(8)：39
[35]李步尧等 中医研究，1995；8(2)：29～30
[36]钱元龙等 南京中医学院学报，1990；6(2)：26～29
[37]王发谓等 河南中医，1994；14(4)：227～228
[38]庞建国等 陕西中医，1991；12(10)：435～436
[39]高振华等 中医杂志，1995；36(8)：460～462
[40]陈泽等 辽宁中医杂志，1990；(1)：8～9
[41]王永炎主编．中医内科学．第六版，上海：上海科技出版社，1997；108
[42]方立琼等 上海医科大学学报 1992；19(2)124
[43]郑虎占等 中药现代研究与应用 北京：学苑出版社 1416
[44]朱接全等 中国药理学通报 1991；7(1)23
[45]夏敬生等 中国药理学报 1999；20：512
[46]朱接全等 中国药理学通报 1986；2(6)21
[47]李淑琴等 实用内科杂志 1988；8(1)22
[48]李贵荣等 中国药理学报 1984：5(1)20
[49]朱接全等 中国药理学报 1986；7(6)543
[50]宗贤刚等 中国药理通讯 1985；6(1)30
[51]李胜男等 中国药理学报 1990：13(6)535
[52]罗湘等 中国动脉粥样硬化杂志等 1995；3(2)177
[53]管菲等 中医药研究 2000；16(5)46～47
[54]朱接全等 中国药理学报 1986；7(6)543
[55]陈淑华等 中国药理学报 1982；3(3)178
[56]曾繁典等 武汉医学院学报 1984：14(3)205
[57]朱接全等 同济医科大学学报 1987：16(5)361
[58]周际安等 中国药理学通报 1994：10(1)33
[59]刘俊田等 西北药学杂志 1988：3(3)6
[60]岳天立等 中国药理学报 1989；10(3)279
[61]胡淑敏等 全国中药和天然药物学术讨论会论文摘要集 1989：41
[62]刘俊田等 西安医科大学学报 1991；12(1)33
[63]曾国钱等 药学学报 1993；28(7)499
[64]郑虎占等 中药现代化研究与应用 第二卷 1417—1423
[65]杜佐华等 同济医科大学学报 1986；15(3)212
[66]杜佐华等 中国药理通讯 1990；7(1)18
[67]王光祖等 中国药理学与毒理学杂志 1992：6(2)37
[68]杜耀辉等 中国药理学报 1993：14(2)173
[69]代宗顺等 药学通报 1983；18(5)22
[70]侯淑贤等 药学学报 1982：17(11)863
[71]代宗顺等 武汉医学院学报 1983；12(3)290
[72]代宗顺等 中国临床药理学杂志 1987；3(4)256
[73]王星光等 中药药理与临床 1989：5(6)：21—22
[74]李仪奎等 中药药理实验方法学 上海科技出版社 1991；122—124
[2]高血压与冠心病 全国心血管防治研究办公室编 科学出版社
[3]陈纪林 中华心血管病杂志 1997、25(1)70～71
[4]Reimer KA et al, Four brief Periiods of myocardial ischemic cause no cumulative ATP loss or necrosia Am J physio、1986, 251:H1306
[5]Murry CE, Jennings RB, Reimer KA, et al、preconditioning with ischemia: adelay of lethal cell injury ischemic myocardium circulation, 1986、74(5)1124
[6]Murry CE et al. Ischemic preconditioning slows energy metabolism and delays ultrastructural damage dauring a sustaind ischemic episode circ Res, 1990,66:913
[7]Marber MS et al. circulation 1993; 88: 1264～1272
[8]Baxter CF et al. circulation. 1995;92(suppl I)I--389
[9]Thornton JD et al. Intravenous pretreatment with A_1 sclective alenosine analogues protects the heart against infarction circulatin、1992,85:659
[10]Van wylen PGL Effect of ischmic preconditioning on interestial purime metabolite and loctate a ccumulation dauring myocardial ischemic circulatior 1994,89(5)2238
[11]Liu Y, Downey JM preconditioning against infarction in the rat heart does not involve pertussis toxin sensitive G, protein cardiovasc Res, 1993,27:68
[12]Klaus v, Kan Y, Keijo Pet al. mechanisms of ischemic proconditioning in rat myocardium、circulation 1995,91(5):2810
[13]陈莹莹等 心血管病学进展 1997、18(5)271～276
[14]Baner jeea, Locke winter, Rogers KB, et al, preconditioning against myocardial dysfunction after ischemia and reperfusion by an alpna 1-adrenergic mechanism cir Res, 1993,73(4)656
[15]Noda k, sasayui M, et al, Role of locally formed angiotensinⅡand
bredykinin in the reduction of myocardial infarct size in dogs,cardiovasc Res 1993,27 (2) :334
[16] Liu Y,Tauchida A,cohen Mv et al、 pretreatment with angiotensi II activates protein kinasec and limits myocardial infarction in isclated rabbit hearts,J Mol cell cardiol,1995,27 (3) 883
[17] Richard v,Blauc T,Kaeffer N et al、 myocardial and cornary end othelial protective effects of occetylchdine after myocardial ischemia and reperfusion in rats:role of nitric oxide Br、 J Pharmacol,1995,115(8) 1532
[18] 赵丽娟 中国乡村医生杂志 1994、 7, 16-19
[19] Southorn PA, mayo clin proc,1998,63:381
[20] Thompson JA,Hess ML、 the oxygen free radical system:a fundamental mechanism in the production of myocardial necrosis, prog cardiovasc Dis,1986;26(6) :449
[21] She AC、 Jennings RB,kinetics of calcium accumlation in acute myocardial ischemic injury[J]、 AMJ Rathol,1972,67:441-447
[22] Ikeda u,Arisaka H,Takeda K, et al、 protein kinase catetivation aggrevate hyposic myocardial injury by stimulating Na+/H+ exchange[J]、 Mol cell cardiol 1988,20:493-498
[23] Xu X, star RA, Tortorici G, et al、 Depletion of interacellular Ca2+store activantes nitricoxide synthase to generate cGMP and regulate Ca2+ influx [J].J Biol chem 1994,269:12645-2653
[24] Brauwald E, Kloner R、 the stunned myocardium:prolonged,postischemic ventricular dysfunction、 circulation,1982,6,1146
[25] 唐强 心血管病学进展 2002,21(1) 15-18
[26] Rahimthoola SH, the hibernating myocardium. Am Heart J 1989,17:211
[27] Boden WE,Brooks WW conrad CH, et al,Incomplete delaved functional recovery late after reperfusion following acute myocardial infaraction " myocardium" AM Heart J,1995,130:922
[28] Ferrari R,La canna G,Ginbbini R,et al,left ventricnlar dysfunction due to
stunning and hibernation in patients cadiovas Drugs Ther, 1994,8:371
[29]Sabia P J、poers ER、Ragosta M,et al,Am associated between collateral blood folow and myocardial viability in patients with recent myocardial infarction、N Engl J Med,1992,327:1825
[30]杨维益等 浙江中医杂志，1981；(9)：386
[31]姚真敏等 新中医，1986；(4)：13
[32]闫洪臣等 陕西新医药，1979；(6)：49
[33]宋崇顺等 辽宁中医杂志，1987；(5)：42
[34]翁维良等 中医杂志，1989；(8)：39
[35]李步尧等 中医研究，1995；8(2)：29～30
[36]钱元龙等 南京中医学院学报，1990；6(2)：26～29
[37]王发谓等 河南中医，1994；14(4)：227～228
[38]庞建国等 陕西中医，1991；12(10)：435～436
[39]高振华等 中医杂志，1995；36(8)：460～462
[40]陈泽等 辽宁中医杂志，1990；(1)：8～9
[41]王永炎主编．中医内科学．第六版，上海：上海科技出版社，1997；108
[42]方立琼等 上海医科大学学报 1992；19(2)124
[43]郑虎占等 中药现代研究与应用 北京：学苑出版社 1416
[44]朱接全等 中国药理学通报 1991；7(1)23
[45]夏敬生等 中国药理学报 1999；20：512
[46]朱接全等 中国药理学通报 1986；2(6)21
[47]李淑琴等 实用内科杂志 1988；8(1)22
[48]李贵荣等 中国药理学报 1984：5(1)20
[49]朱接全等 中国药理学报 1986；7(6)543
[50]宗贤刚等 中国药理通讯 1985；6(1)30
[51]李胜男等 中国药理学报 1990：13(6)535
[52]罗湘等 中国动脉粥样硬化杂志等 1995；3(2)177
[53]管菲等 中医药研究 2000；16(5)46～47
[54]朱接全等 中国药理学报 1986；7(6)543
[55]陈淑华等 中国药理学报 1982；3(3)178
[56]曾繁典等 武汉医学院学报 1984：14(3)205
[57]朱接全等 同济医科大学学报 1987：16(5)361
[58]周际安等 中国药理学通报 1994：10(1)33
[59]刘俊田等 西北药学杂志 1988：3(3)6
[60]岳天立等 中国药理学报 1989；10(3)279
[61]胡淑敏等 全国中药和天然药物学术讨论会论文摘要集 1989：41
[62]刘俊田等 西安医科大学学报 1991；12(1)33
[63]曾国钱等 药学学报 1993；28(7)499
[64]郑虎占等 中药现代化研究与应用 第二卷 1417—1423
[65]杜佐华等 同济医科大学学报 1986；15(3)212
[66]杜佐华等 中国药理通讯 1990；7(1)18
[67]王光祖等 中国药理学与毒理学杂志 1992：6(2)37
[68]杜耀辉等 中国药理学报 1993：14(2)173
[69]代宗顺等 药学通报 1983；18(5)22
[70]侯淑贤等 药学学报 1982：17(11)863
[71]代宗顺等 武汉医学院学报 1983；12(3)290
[72]代宗顺等 中国临床药理学杂志 1987；3(4)256
[73]王星光等 中药药理与临床 1989：5(6)：21—22
[74]李仪奎等 中药药理实验方法学 上海科技出版社 1991；122—124