BRG1在DNA损伤修复中的作用研究
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摘要
染色质高度紧密的折叠阻止了转录因子和辅因子与DNA的结合,因而通过染色质重塑以解除这样的抑制环境,对于转录活动的正常进行是至关重要的。目前,染色质重塑至少是通过两种机制来完成的,一是通过依赖于ATP的染色质改构复合物,另一是通过组蛋白修饰酶复合物。前者的典型代表是SWI/SNF复合物,人类以BRG1或BRM作为其ATP酶催化亚基,BRG1在基因的转录调控、复制、重组等方面起着重要的作用,但该亚基在DNA损伤修复中所起的作用还不是很清楚。
DNA分子是生命体的遗传物质基础,细胞内的DNA分子因物理、化学等多种因素的作用,有可能导致多种类型的损伤。当DNA受到紫外线照射时,主要产生环丁烷-嘧啶酮二聚体(CPD)和嘧啶6-4嘧啶酮光产物(6-4PP)。对于UV照射引起的DNA损伤主要是通过核酸切除修复途径进行修复的。
本文主要讨论了在UV照射引起的DNA损伤条件下,BRG1与DNA损伤修复的关系。首先建立UV照射引起的DNA损伤模型,即通过不同剂量的UV照射,并采用流式细胞术对细胞凋亡程度进行检测,用以摸索适合的UV剂量从而建立该模型。在该模型的基础上,通过在SW13细胞中瞬时转染BRG1表达质粒及空载pBJ5质粒(对照组),经过30J/m2的UV照射后,分别以0h、6h、24h为修复时间进行细胞损伤后修复,最后对细胞早期凋亡程度进行检测。经过统计分析,结果表明,转染了BRG1表达质粒的细胞的早期凋亡程度明显较对照组的程度低,尤其是经过24h的修复后,说明BRG1的参与促进了DNA的损伤修复。这一结果为BRG1在DNA损伤修复中所起作用的研究奠定了一定的实验基础,也为今后深入探索BRG1在该领域中具体的作用机制提供了线索,更有助于了全面深入地理解改构复合物在细胞内扮演的各种重要角色。
The highly condensed chromatin prevents the binding of transcription factors and cofactors to DNA. Therefore, it’s crucial to relief the repressive environment for transcription through chromatin remodeling activities. Recently, chromatin remodeling is carried out by at least two mechanisms: ATP-dependent chromatin remodeling complexes and histone modification complexes. The representive of the former one is the SWI/SNF comlex, the ATPase subunit of which is BRG1 or BRM in human. BRG1 plays an important role in the regulation of gene transcription, replication, recombination and etal. However, it`s still unclear that the function BRG1 carries out in DNA damage and repair.
DNA is the basis of genetic material, which may give rise to some kinds of damage in the existence of physical and chemical factors. CPD and 6-4PP are the main photoproducts after UV irradiation. For this kind of damage, nucleutide excision repair (NER) is the major repair pathway.
In this article, we mainly discussed the relationship between the BRG1 and DNA damage and repair. Firstly, we set up a UV-induced DNA damage and repair model through distinct dose of UV irradiation and tests to the cellular apoptosis by flow cytometry in order to find suitable UV dose. On the basis of this model, BRG1 expression plasmid and vector plasmid pBJ5 were transfected separately into SW13, which would repair themselves during the distinct repair time of 0h, 6h and 24h after 30J/m2 UV irradiation, then tests were carried out to justify the content of primary cellular apoptosis. The results showed that the percentage of primary cellular apoptosis of SW13 with transfection of BRG1 expression plasmid was lower obviously than that with transfection of vector plasmid pBJ5, especially after the repair time of 24h. All of these above indicated that BRG1 took part in and promoted DNA damage and repair. The results made foundation for the research of the role of BRG1 on DNA damage and repair, also provided clues for further exploring the specific mechanism of BRG1 on this field, and help us to understand generally about varies important roles the chromatin-remodeling complexes play in cells.
DNA分子是生命体的遗传物质基础,细胞内的DNA分子因物理、化学等多种因素的作用,有可能导致多种类型的损伤。当DNA受到紫外线照射时,主要产生环丁烷-嘧啶酮二聚体(CPD)和嘧啶6-4嘧啶酮光产物(6-4PP)。对于UV照射引起的DNA损伤主要是通过核酸切除修复途径进行修复的。
本文主要讨论了在UV照射引起的DNA损伤条件下,BRG1与DNA损伤修复的关系。首先建立UV照射引起的DNA损伤模型,即通过不同剂量的UV照射,并采用流式细胞术对细胞凋亡程度进行检测,用以摸索适合的UV剂量从而建立该模型。在该模型的基础上,通过在SW13细胞中瞬时转染BRG1表达质粒及空载pBJ5质粒(对照组),经过30J/m2的UV照射后,分别以0h、6h、24h为修复时间进行细胞损伤后修复,最后对细胞早期凋亡程度进行检测。经过统计分析,结果表明,转染了BRG1表达质粒的细胞的早期凋亡程度明显较对照组的程度低,尤其是经过24h的修复后,说明BRG1的参与促进了DNA的损伤修复。这一结果为BRG1在DNA损伤修复中所起作用的研究奠定了一定的实验基础,也为今后深入探索BRG1在该领域中具体的作用机制提供了线索,更有助于了全面深入地理解改构复合物在细胞内扮演的各种重要角色。
The highly condensed chromatin prevents the binding of transcription factors and cofactors to DNA. Therefore, it’s crucial to relief the repressive environment for transcription through chromatin remodeling activities. Recently, chromatin remodeling is carried out by at least two mechanisms: ATP-dependent chromatin remodeling complexes and histone modification complexes. The representive of the former one is the SWI/SNF comlex, the ATPase subunit of which is BRG1 or BRM in human. BRG1 plays an important role in the regulation of gene transcription, replication, recombination and etal. However, it`s still unclear that the function BRG1 carries out in DNA damage and repair.
DNA is the basis of genetic material, which may give rise to some kinds of damage in the existence of physical and chemical factors. CPD and 6-4PP are the main photoproducts after UV irradiation. For this kind of damage, nucleutide excision repair (NER) is the major repair pathway.
In this article, we mainly discussed the relationship between the BRG1 and DNA damage and repair. Firstly, we set up a UV-induced DNA damage and repair model through distinct dose of UV irradiation and tests to the cellular apoptosis by flow cytometry in order to find suitable UV dose. On the basis of this model, BRG1 expression plasmid and vector plasmid pBJ5 were transfected separately into SW13, which would repair themselves during the distinct repair time of 0h, 6h and 24h after 30J/m2 UV irradiation, then tests were carried out to justify the content of primary cellular apoptosis. The results showed that the percentage of primary cellular apoptosis of SW13 with transfection of BRG1 expression plasmid was lower obviously than that with transfection of vector plasmid pBJ5, especially after the repair time of 24h. All of these above indicated that BRG1 took part in and promoted DNA damage and repair. The results made foundation for the research of the role of BRG1 on DNA damage and repair, also provided clues for further exploring the specific mechanism of BRG1 on this field, and help us to understand generally about varies important roles the chromatin-remodeling complexes play in cells.
引文
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