纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维（nHAC/PLLA/CF）对骨缺损的治疗动物实验
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摘要
目的:探讨纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维(nHAC/PLLA/CF)复合材料和纳米羟基磷灰石/聚-L-乳酸(nHAC/PLLA)两种支架的成骨性能;比较纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料和纳米羟基磷灰石/聚-L-乳酸两种支架在成骨性能和生物降解方面的差异,揭示影响材料成骨性能和生物降解的相关因素。
方法:健康新西兰白兔6只,由吉林大学实验动物中心提供,6-8周龄,体重1.5-2.0Kg,雌雄不限。随机分为2组,全部6只右侧桡骨植入纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料。左侧桡骨:空白对照组为2只,纳米羟基磷灰石/聚-L-乳酸组为4只。术后分别于4,8周将A,B组动物通过注射过量速眠新的方式处死,每次每组各处死3只,取材范围包括材料及其周围1cm正常骨组织。
结果:
1.X线检查纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组和纳米羟基磷灰石/聚-L-乳酸组在4周时均显示骨断端内形成高密度骨组织阴影,提示有钙化的骨组织。纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组骨连结比纳米羟基磷灰石/聚-L-乳酸组明显,而对照组则行成骨不连接。8周时纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组达到骨愈合,纳米羟基磷灰石/聚-L-乳酸组基本达到骨愈合,而对照组仍旧为骨不连。
2.组织学检查纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组和纳米羟基磷灰石/聚-L-乳酸组在4周时,大体标本均见骨折端已行成骨性连接,其中纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组更明显一些,而对照组则行成骨不连。8周时纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维糖复合材料组的骨化结节中的软基质已基本完成骨化过程,骨细胞的体积较小。骨化结节周围有一层致密的纤维组织覆盖。纳米羟基磷灰石/聚-L-乳酸材料组的骨化结节中的软基质骨化过程未完成,仅见少量骨细胞。而对照组则行成骨不连。
结论:纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料具有良好的修复骨缺损能力,同时也拥有较好的生物学效应,可以在于治疗骨缺损中替代自体骨。
Objective: to investigate osteogenetic ability and biological response of three kinds of biomaterial---- nHAC/PLLA/CF; comparing difference of osteogenetic ability and biodegradation between three different biomaterial thereby can we reveal some factors in related to osteogenesis ability and biodegradation of three biomaterial acting as bone replacement.
Method: 6 New Zealand rabbits were offer by the animal laboratory of Jinlin Universtity .They were 6-8 weeks old that weigh 1.5-2.0Kg.There were no difference between male or female.Then they were divided into two groups by chance. The right radial bone were embed the material of nHAC/PLLA/CF . The left radial bone :there were two blanks for comparison and four for nHAC/PLLA/CF. Specimens harvested in4w and 8w postoperation were further assessed on the two groups. Specimens harvested on 3 rabbits every time. Sampling should include the material and the normal bone around 1cm.
Result : 1. Use X line to check Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid chitin fiber compound material set and the Na rice Qian radicle apatite/gather-L-lactic acid set at all show that the bone’s break to carry for 4 weeks inside formed a high definition bone organization shadow. There was a hint to have the bone organization of calcify. The Na rice Qian radicle apatite / the original gum /gather-L-lactic acid chitin fiber compound material set bone link compare the Na rice Qian radicle apatite/gather-the L-lactic acid set is obvious, while the matched control then go ossification which is not conjunction. In the 8 wees, the Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid/chitin fiber compound the material set attain a bone to heal, the Na rice Qian radicle apatite/gather-the L-lactic acid set Be basic to attain a bone to heal, while the matched control is still a bone not connecting .
2.The histology checks the Na rice Qian radicle apatite/the original gum /gather-L-lactic acid/chitin fiber compound material set and the Na rice Qian radicle apatite/gather-L-lactic acid set at 4 weeks. The ossification conjunction of the specimen can be seen, and among them the Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid/chitin fiber compound the material set is more obviously, while the matched group’s ossification conjunction was not obvious.8 weeks later,the Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid/chitin fiber sugar compound material set of bone's turning to knot the soft radicle quality in the stanza a basic completion bone have already turned process, the physical volume of bone cell is smaller. The bone turns knot stanza to around have the fine fiber organization the overlay. The Na rice Qian radicle apatite/|gather-the bone of the L-lactic acid material set turn to knot the soft radicle quality bone in the stanza to turn process not yet finished and only see a little amount bone cell. But the matched groups are not connected.
Conclusion: the Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid/chitin fiber compound the material has a good repair bone defection and damage ability, also own better biology effect in the meantime. They can lie in curing in the bone defection and damage and act for from the body bone.
方法:健康新西兰白兔6只,由吉林大学实验动物中心提供,6-8周龄,体重1.5-2.0Kg,雌雄不限。随机分为2组,全部6只右侧桡骨植入纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料。左侧桡骨:空白对照组为2只,纳米羟基磷灰石/聚-L-乳酸组为4只。术后分别于4,8周将A,B组动物通过注射过量速眠新的方式处死,每次每组各处死3只,取材范围包括材料及其周围1cm正常骨组织。
结果:
1.X线检查纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组和纳米羟基磷灰石/聚-L-乳酸组在4周时均显示骨断端内形成高密度骨组织阴影,提示有钙化的骨组织。纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组骨连结比纳米羟基磷灰石/聚-L-乳酸组明显,而对照组则行成骨不连接。8周时纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组达到骨愈合,纳米羟基磷灰石/聚-L-乳酸组基本达到骨愈合,而对照组仍旧为骨不连。
2.组织学检查纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组和纳米羟基磷灰石/聚-L-乳酸组在4周时,大体标本均见骨折端已行成骨性连接,其中纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料组更明显一些,而对照组则行成骨不连。8周时纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维糖复合材料组的骨化结节中的软基质已基本完成骨化过程,骨细胞的体积较小。骨化结节周围有一层致密的纤维组织覆盖。纳米羟基磷灰石/聚-L-乳酸材料组的骨化结节中的软基质骨化过程未完成,仅见少量骨细胞。而对照组则行成骨不连。
结论:纳米羟基磷灰石/胶原/聚-L-乳酸/甲壳素纤维复合材料具有良好的修复骨缺损能力,同时也拥有较好的生物学效应,可以在于治疗骨缺损中替代自体骨。
Objective: to investigate osteogenetic ability and biological response of three kinds of biomaterial---- nHAC/PLLA/CF; comparing difference of osteogenetic ability and biodegradation between three different biomaterial thereby can we reveal some factors in related to osteogenesis ability and biodegradation of three biomaterial acting as bone replacement.
Method: 6 New Zealand rabbits were offer by the animal laboratory of Jinlin Universtity .They were 6-8 weeks old that weigh 1.5-2.0Kg.There were no difference between male or female.Then they were divided into two groups by chance. The right radial bone were embed the material of nHAC/PLLA/CF . The left radial bone :there were two blanks for comparison and four for nHAC/PLLA/CF. Specimens harvested in4w and 8w postoperation were further assessed on the two groups. Specimens harvested on 3 rabbits every time. Sampling should include the material and the normal bone around 1cm.
Result : 1. Use X line to check Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid chitin fiber compound material set and the Na rice Qian radicle apatite/gather-L-lactic acid set at all show that the bone’s break to carry for 4 weeks inside formed a high definition bone organization shadow. There was a hint to have the bone organization of calcify. The Na rice Qian radicle apatite / the original gum /gather-L-lactic acid chitin fiber compound material set bone link compare the Na rice Qian radicle apatite/gather-the L-lactic acid set is obvious, while the matched control then go ossification which is not conjunction. In the 8 wees, the Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid/chitin fiber compound the material set attain a bone to heal, the Na rice Qian radicle apatite/gather-the L-lactic acid set Be basic to attain a bone to heal, while the matched control is still a bone not connecting .
2.The histology checks the Na rice Qian radicle apatite/the original gum /gather-L-lactic acid/chitin fiber compound material set and the Na rice Qian radicle apatite/gather-L-lactic acid set at 4 weeks. The ossification conjunction of the specimen can be seen, and among them the Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid/chitin fiber compound the material set is more obviously, while the matched group’s ossification conjunction was not obvious.8 weeks later,the Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid/chitin fiber sugar compound material set of bone's turning to knot the soft radicle quality in the stanza a basic completion bone have already turned process, the physical volume of bone cell is smaller. The bone turns knot stanza to around have the fine fiber organization the overlay. The Na rice Qian radicle apatite/|gather-the bone of the L-lactic acid material set turn to knot the soft radicle quality bone in the stanza to turn process not yet finished and only see a little amount bone cell. But the matched groups are not connected.
Conclusion: the Na rice Qian radicle apatite/ the original gum /gather-L-lactic acid/chitin fiber compound the material has a good repair bone defection and damage ability, also own better biology effect in the meantime. They can lie in curing in the bone defection and damage and act for from the body bone.
引文
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[2]Arrington ED Smith WJ , Chambers HG, et al. Complications of iliac crest bone graft harvesting .Clin Orthop 1996;329: 300-9.
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[7]Palmer SH,Gibbons CL,Athanasou NA. The pathology of bone allograft .J Bone Joint Surg Brl999:81:333-5.
[8]Friedlaender GE,Strong DM,Tomford W,Manikin HJ .Long term follow-up of patients with osteochondral allograft .A correlation between immunologic responses and clinical outcome .Orthop Clin North Am 1999; 30:583- 8.
[9]Herman P,Finlayson D. Ordering allograft by weight :suggestions forimpaction grafting .J Arthroplasty 2000; 15:368-71.
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