JAK2/STAT3通路对小鼠骨骼肌发育、能量代谢及肌间脂肪相关基因表达的影响
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摘要
JAK2激酶/信号转导和转录激活因子3(JAK2/STAT3)信号通路不仅具有心肌保护作用,在细胞生长、分化及凋亡过程中也发挥重要作用,还可调节动物体脂肪的代谢。但有关该途径在骨骼肌上的研究少有报道。本研究以健康昆明小白鼠和小白鼠骨骼肌细胞为研究对象,分别用AG490和IL-11处理。称取小鼠体重、测量小鼠体温及检测氧化酶(SOD、CAT和GSH-PX)活性和MDA含量,利用Real-time PCR技术检测JAK2、STAT3、MyoD、Myf5、LXRα、UCP3、PPARγ、FAS和HSL基因表达,分别在个体和细胞水平上,分析该通路对小鼠骨骼肌发育、能量代谢相关基因表达及氧化酶活性的影响。
1用JAK2特异性抑制剂AG490处理小鼠,处理组小鼠体重显著低于对照组(P<0.05);小鼠体温维持在正常浮动范围内;JAK2和STAT3基因表达显著下降(P<0.05);骨骼肌发育相关基因MyoD和Myf5表达极显著下降(P<0.01);能量代谢相关基因LXRα和UCP3表达极显著下降(P<0.01)。表明AG490有效地抑制了骨骼肌组织中JAK2/STAT3信号通路,可通过降低骨骼肌发育和能量代谢相关基因的表达而调节小鼠骨骼肌发育和能量代谢。
2用IL-11处理小鼠,处理组小鼠体重显著高于对照组(P<0.05);小鼠体温维持在正常浮动范围内;JAK2和STAT3表达显著升高(P<0.05)。骨骼肌发育相关基因MyoD和Myf5表达显著上升(P<0.05);能量代谢相关基因LXRα表达无变化(P>0.05),UCP3表达显著上升(P<0.05)。证明IL-11可以激活JAK2/STAT3信号通路,可通过提高骨骼肌发育和能量代谢相关基因的表达而调节骨骼肌发育和能量代谢。
3分别用IL-11和AG490处理小鼠,IL-11处理组小鼠脂肪代谢相关基因PPARγ表达显著高于对照组(P<0.05),FAS和HSL表达无变化(P>0.05);CAT、GST-PX和SOD酶活性显著增强(P<0.05),MDA含量显著下降(P<0.05)。AG490处理组,PPARγ、FAS和HSL表达显著下降(P<0.05);CAT、GST-PX和SOD酶活性显著下降(P<0.05),但MDA含量却显著增加。证明JAK2/STAT3信号通路可能参与调节肌间脂肪的沉积,也对机体组织的酶活产生影响,起到保护机体组织的作用。
4 15μg/mL AG490和10ng/mL IL-11分别作用于骨骼肌细胞。AG490处理组JAK2和STAT3表达显著下降(P<0.05);骨骼肌发育相关基因MyoD和Myf5表达显著下降(P<0.05);能量代谢相关基因LXRа和UCP3表达显著下降(P<0.05)。IL-11处理组JAK2和STAT3表达显著上升(P<0.05);骨骼肌发育相关基因MyoD和Myf5表达显著上升(P<0.05);能量代谢相关基因LXRа表达无变化(P<0.05),UCP3表达显著上升(P<0.05)。从细胞水平上证明JAK2/STAT3信号通路对骨骼肌发育和能量代谢相关基因表达产生影响。
Janus Kinase2/Signal transducers and activators of transcription3 (JAK2/STAT3) signal pathway is a recent research focus, which has myocardial protect function and plays an important role in cell growth, differentiation and apoptosis. In addition, the pathway could regulate metabolism of animal fat. But there are less research on this pathway in skeletal muscles. In this study, Kun Ming mice and mouse skeletal muscle were taken as study object, which were treated with AG490 and IL-11. Body weight change, body temperature and oxidative enzymes (SOD、CAT、MDA and GSH-PX) activity were measured, and JAK2、STAT3、MyoD、Myf5、LXRα、UCP3、PPARγ、FAS and HSL gene expression were detected by using Real-time PCR, to analysis the effect of JAK2/STAT3 signaling pathway in mouse skeletal muscle development, energy metabolism and oxidative enzyme activity.
1 To study the effects of JAK2/STAT3 signal pathway on the expression of muscle development and energy metabolic related genes by inhibiting this pathway using AG490. The tentative male Kunming mice were treated with intraperitoneal injection of AG490 for two weeks. After execution, the leg muscle was surgically removed and total RNA was extracted. Real-time quantitative PCR was used to examine the expression of the key factors in JAK2/STAT3 signal pathway (JAK2、STAT3), the growth related genes of skeletal muscle(MyoD、Myf5), and energy metabolic related gene(sLXRα、UCP3). Compared with control, in the treatment group, the body weight of mice were significantly decreased (P<0.05); the body temperature was stable; the expression of JAK2 and STAT3 were significantly decreased(P<0.05); the expression level of LXRα、UCP3、MyoD and Myf5 were decreased extremely significant (P<0.01). JAK2/STAT3 signal pathway might regulate the growth of skeletal muscle and energy metabolism by decreasing the expression levels of the growth of skeletal muscle development and energy metabolic related genes.
2 To study the effects of JAK2/STAT3 signal pathway on the expression of muscle development and energy metabolic related genes by activating this pathway using IL-11. The tentative male Kunming mice were treated with intraperitoneal injection of IL-11 for two weeks. After execution, the leg muscle was surgically removed and total RNA was extracted. Real-time quantitative PCR was used to examine the expression of the key factors in JAK2/STAT3 signal pathway, JAK2 and STAT3, the growth related genes MyoD and Myf5, and energy metabolic related genes LXRαand UCP3. Compared with control group, the mice weight of muscle and body were significantly increased in the treatment groups (P<0.05); body temperature was stable in the reasonable rang; the expression of STAT3 and JAK2 were significantly increased (P<0.05); the expression level of MyoD和Myf5 were significantly increased(P<0.05); the expression of LXRαwas slightly up-regulated (P>0.05)and UCP3 expression increased (P<0.05). The results indicated that JAK2/STAT3 signal pathway might regulate the growth of skeletal muscle and energy metabolism by increasing the expression levels of the skeletal muscle development and energy metabolic related genes.
3 Mice were respectively treated with IL-11 and AG490. The results showed that in IL-11 treatment group, the expression of genes PPARγincreased significantly (P<0.05), FAS and HSL expression were unchanged (P>0.05); CAT、GST-PX and SOD activity decreased significantly (P<0.05), MDA content was significantly increased (P<0.05). In AG490 treatment group, PPARγ、FAS and HSL expression decreased significantly (P<0.05); CAT、GST-PX and SOD activity significantly decreased (P<0.05), but the MDA content significantly increased. The results showed that JAK2/STAT3 signaling pathway may be involved in regulating muscle fat deposition, Also affect the activity of tissues of the body play an important role in protect the organization.
4 Skeletal muscle cells were respetively treated with 15μg/mL AG490 and 10 ng/mL IL-11, In AG490 treatment group, JAK2 and STAT3 expression significantly decreased (P<0.05); MyoD and Myf5 expression significantly decreased (P<0.05); LXRаand UCP3 expression significantly decreased (P<0.05). In IL-11 treatment group, JAK2 and STAT3 expression significantly increased (P<0.05); MyoD and Myf5 expression increased significantly (P<0.05); LXRаgene expression did not change significantly(P>0.05), UCP3 expression significantly increased (P<0.05). The results certificated that JAK2/STAT3 pathway relates to skeletal muscle development and energy metabolism at the cell level.
1用JAK2特异性抑制剂AG490处理小鼠,处理组小鼠体重显著低于对照组(P<0.05);小鼠体温维持在正常浮动范围内;JAK2和STAT3基因表达显著下降(P<0.05);骨骼肌发育相关基因MyoD和Myf5表达极显著下降(P<0.01);能量代谢相关基因LXRα和UCP3表达极显著下降(P<0.01)。表明AG490有效地抑制了骨骼肌组织中JAK2/STAT3信号通路,可通过降低骨骼肌发育和能量代谢相关基因的表达而调节小鼠骨骼肌发育和能量代谢。
2用IL-11处理小鼠,处理组小鼠体重显著高于对照组(P<0.05);小鼠体温维持在正常浮动范围内;JAK2和STAT3表达显著升高(P<0.05)。骨骼肌发育相关基因MyoD和Myf5表达显著上升(P<0.05);能量代谢相关基因LXRα表达无变化(P>0.05),UCP3表达显著上升(P<0.05)。证明IL-11可以激活JAK2/STAT3信号通路,可通过提高骨骼肌发育和能量代谢相关基因的表达而调节骨骼肌发育和能量代谢。
3分别用IL-11和AG490处理小鼠,IL-11处理组小鼠脂肪代谢相关基因PPARγ表达显著高于对照组(P<0.05),FAS和HSL表达无变化(P>0.05);CAT、GST-PX和SOD酶活性显著增强(P<0.05),MDA含量显著下降(P<0.05)。AG490处理组,PPARγ、FAS和HSL表达显著下降(P<0.05);CAT、GST-PX和SOD酶活性显著下降(P<0.05),但MDA含量却显著增加。证明JAK2/STAT3信号通路可能参与调节肌间脂肪的沉积,也对机体组织的酶活产生影响,起到保护机体组织的作用。
4 15μg/mL AG490和10ng/mL IL-11分别作用于骨骼肌细胞。AG490处理组JAK2和STAT3表达显著下降(P<0.05);骨骼肌发育相关基因MyoD和Myf5表达显著下降(P<0.05);能量代谢相关基因LXRа和UCP3表达显著下降(P<0.05)。IL-11处理组JAK2和STAT3表达显著上升(P<0.05);骨骼肌发育相关基因MyoD和Myf5表达显著上升(P<0.05);能量代谢相关基因LXRа表达无变化(P<0.05),UCP3表达显著上升(P<0.05)。从细胞水平上证明JAK2/STAT3信号通路对骨骼肌发育和能量代谢相关基因表达产生影响。
Janus Kinase2/Signal transducers and activators of transcription3 (JAK2/STAT3) signal pathway is a recent research focus, which has myocardial protect function and plays an important role in cell growth, differentiation and apoptosis. In addition, the pathway could regulate metabolism of animal fat. But there are less research on this pathway in skeletal muscles. In this study, Kun Ming mice and mouse skeletal muscle were taken as study object, which were treated with AG490 and IL-11. Body weight change, body temperature and oxidative enzymes (SOD、CAT、MDA and GSH-PX) activity were measured, and JAK2、STAT3、MyoD、Myf5、LXRα、UCP3、PPARγ、FAS and HSL gene expression were detected by using Real-time PCR, to analysis the effect of JAK2/STAT3 signaling pathway in mouse skeletal muscle development, energy metabolism and oxidative enzyme activity.
1 To study the effects of JAK2/STAT3 signal pathway on the expression of muscle development and energy metabolic related genes by inhibiting this pathway using AG490. The tentative male Kunming mice were treated with intraperitoneal injection of AG490 for two weeks. After execution, the leg muscle was surgically removed and total RNA was extracted. Real-time quantitative PCR was used to examine the expression of the key factors in JAK2/STAT3 signal pathway (JAK2、STAT3), the growth related genes of skeletal muscle(MyoD、Myf5), and energy metabolic related gene(sLXRα、UCP3). Compared with control, in the treatment group, the body weight of mice were significantly decreased (P<0.05); the body temperature was stable; the expression of JAK2 and STAT3 were significantly decreased(P<0.05); the expression level of LXRα、UCP3、MyoD and Myf5 were decreased extremely significant (P<0.01). JAK2/STAT3 signal pathway might regulate the growth of skeletal muscle and energy metabolism by decreasing the expression levels of the growth of skeletal muscle development and energy metabolic related genes.
2 To study the effects of JAK2/STAT3 signal pathway on the expression of muscle development and energy metabolic related genes by activating this pathway using IL-11. The tentative male Kunming mice were treated with intraperitoneal injection of IL-11 for two weeks. After execution, the leg muscle was surgically removed and total RNA was extracted. Real-time quantitative PCR was used to examine the expression of the key factors in JAK2/STAT3 signal pathway, JAK2 and STAT3, the growth related genes MyoD and Myf5, and energy metabolic related genes LXRαand UCP3. Compared with control group, the mice weight of muscle and body were significantly increased in the treatment groups (P<0.05); body temperature was stable in the reasonable rang; the expression of STAT3 and JAK2 were significantly increased (P<0.05); the expression level of MyoD和Myf5 were significantly increased(P<0.05); the expression of LXRαwas slightly up-regulated (P>0.05)and UCP3 expression increased (P<0.05). The results indicated that JAK2/STAT3 signal pathway might regulate the growth of skeletal muscle and energy metabolism by increasing the expression levels of the skeletal muscle development and energy metabolic related genes.
3 Mice were respectively treated with IL-11 and AG490. The results showed that in IL-11 treatment group, the expression of genes PPARγincreased significantly (P<0.05), FAS and HSL expression were unchanged (P>0.05); CAT、GST-PX and SOD activity decreased significantly (P<0.05), MDA content was significantly increased (P<0.05). In AG490 treatment group, PPARγ、FAS and HSL expression decreased significantly (P<0.05); CAT、GST-PX and SOD activity significantly decreased (P<0.05), but the MDA content significantly increased. The results showed that JAK2/STAT3 signaling pathway may be involved in regulating muscle fat deposition, Also affect the activity of tissues of the body play an important role in protect the organization.
4 Skeletal muscle cells were respetively treated with 15μg/mL AG490 and 10 ng/mL IL-11, In AG490 treatment group, JAK2 and STAT3 expression significantly decreased (P<0.05); MyoD and Myf5 expression significantly decreased (P<0.05); LXRаand UCP3 expression significantly decreased (P<0.05). In IL-11 treatment group, JAK2 and STAT3 expression significantly increased (P<0.05); MyoD and Myf5 expression increased significantly (P<0.05); LXRаgene expression did not change significantly(P>0.05), UCP3 expression significantly increased (P<0.05). The results certificated that JAK2/STAT3 pathway relates to skeletal muscle development and energy metabolism at the cell level.
引文
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