摘要
骨骼肌是动物体内最丰富的组织,占肉用动物体重的45%~60%,其生长发育是肉用家畜最重要的性状之一。不同猪种由于来源、长期所处的地理环境以及所接受的饲养方式和选育性状重点的不同,从而形成了各自的种质特性,使其在肌肉相关性状方面存在较大的差异。两个具有不同性状的亲本杂交产生的杂种在肌肉生长、肌肉品质等方面也往往超过其双亲,产生杂种优势的现象。随着人们对基因结构和功能的研究深入,人们已经认识到这些差异及杂种优势现象应是基因差异表达的结果。所以本研究以猪背最长肌为材料,利用抑制消减杂交技术构建了不同猪种(长白与梅山)以及猪杂交亲代与子代(大白与长大)背最长肌间的正反向消减cDNA文库,进行了差异表达基因的筛选、克隆和鉴定,取得了如下结果:
1.以管家基因G3PDH作为消减指标,对每个消减文库的消减效率进行了检测,结果表明4个消减文库的消减效率都达2~5倍以上,有的甚至高达2~(10)倍,这同时也表明了某些特异于两种肌肉组织的差异表达基因的富集效率也达2~5倍以上,说明所构建的消减cDNA文库质量很好。
2.从以梅山猪为Tester、长白猪为Driver和以杂交母本大白猪为Tester、杂种F1长大猪为Driver的消减cDNA文库中我们分别随机挑取了800多个克隆。利用PCR技术进行鉴定,分别获得了759和773个有效阳性克隆,插入片段长度主要分布于150~750bp之间。
3.利用正向消减cDNA、反向未消减cDNA、反向消减cDNA作探针对两消减cDNA文库中的阳性克隆进行了高通量筛选。在以梅山猪为Tester、长白猪为Driver的消减cDNA文库中,我们选取了27个差异表达克隆进行分析,发现共代表14个ESTs,4个在猪中为已知基因,10个在猪中为未知基因,其中7个与人等其他物种的基因有高同源性,3个未找到明显的同源性。在以杂交母本大白猪为Tester、杂种F1长大猪为Driver的消减cDNA文库中,我们选取了24个差异克隆进行分析,发现共代表12个ESTs,5个在猪中为已知基因,7个在猪中为未知基因,但分别与人等其他物种的基因有高的同源性。并利用含内标化的半定量RT-PCR技术进行了验证,结果表明大部分为差异表达。
4.将电脑克隆和SMART技术相结合,成功克隆了7个差异表达全长基因:(1)CCNG1,GenBank登录号为AY974246,cDNA全长2359bp,ORF为888bp;(2)EEF1A,cDNA全长1777bp,ORF为1389bp;(3) ETFB-like,cDNA全长898bp,ORF为765bp;(4) HUMMLC2B,GenBank登录号为AY754870,cDNA全长681bp,ORF为510 bp;(5) PGK1,GenBank登录号为AY677198,cDNA全长1789bp,ORF为1254bp;(6) TXNIP,cDNA全长1880 bp,ORF为1176 bp;(7) CKM,
Skeletal muscle comprises as much as 45%~60% of an animal's body mass. Hence there is much interest in understanding the development, physiology and metabolism of this tissue. Different pig breeds due to the difference of origin, local environment, feeding and selected traits have different intrinsic features, and there is a large difference between their muscle traits. When two breeds that have different traits are crossed, F1 hybrids tend to demonstrate hybrid vigor and have superior muscle traits. Further understanding on gene structure and function has indicated that the trait difference and heterosis are in fact the external exhibition of gene expression and regulation. Thus, we constructed forward and reverse subtracted cDNA libraries between Longissimus muscles from Meishan and Landrace pigs, Fl hybrids Landrace X Yorkshire and their female parents Yorkshire by suppression subtractive hybridization (SSH) technique in order to clone and identify the differentially expressed genes responsible for the trait difference and heterosis. The findings are as follows:1. A housekeeping gene, G3PDH, was used to estimate the subtraction efficiency. In each subtracted cDNA library, G3PDH was subtracted very efficiently at more than 25 folds, and some even near 2~(10) folds, indicating that some differentially expressed genes were also enriched at the same folds and the subtracted cDNA libraries were very successful.2. More than 800 clones were randomly selected from each subtracted cDNA library. By PCR analyses, 759 and 773 positive clones were isolated from the subtracted cDNA libraries, by Meishan as Tester, Landrace as Driver and Yorkshire as Tester, Landrace×Yorkshire as Driver, respectively. Most of all plasmids in the clones contain 150~750 bp inserts.3. By forward subtractive cDNA, reversal unsubtractive cDNA and reversal subtractive cDNA as probes, high-throughput screening was carried out. In the subtracted cDNA
library by Meishan as Tester and Landrace as Driver, 27 differential expressed clones were obtained. Further analyses showed they represent 14 porcine ESTs. Of 4 are known in pig, 7 are unknown in pig but have a high similarity with human and other species genes, and 3 have no significant similarity with known genes. In the subtracted cDNA library by Yorkshire as Tester and LandracexYorshire as Driver, 24 differential expressed clones were obtained. Further analyses showed they represent 12 porcine ESTs. Of 5 are known in pig, 7 are unknown in pig but have a high similarity with human and other species genes. In addition, most of these differential expressed ESTs were subjected to further identification by semi-quantitative RT-PCR analysis with an inner control4. Using in silico cloning and SMART technology, we obtained 7 full-length cDNA: (1) CCNGl, GenBank accession number AY974246, full-length cDNA 2359 bp, ORF 888bp; (2) EEF1A, full-length cDNA 1777 bp, ORF 1389 bp; (3) ETFB-like, full-length cDNA 898 bp, ORF 765bp; (4) HUMMLC2B, GenBank accession number AY754870, full-length cDNA 681bp, ORF 510 bp; (5) PGK1, GenBank accession number AY677198, full-length cDNA 1789 bp, ORF 1254 bp; (6) TXNIP, full-length cDNA 1880 bp, ORF 1176 bp; (7) CKM, GenBank accession number AY754869, full-length cDNA 1572 bp, ORF 1146 bp. CCNGl, EEF1A and ETFB-like demonstrate high-level expression in Meishan pigs. HUMMLC2B, PGK1, TXNIP and CKM demonstrate down-regulated expression in Fl hybrid LandracexYorshire pigs.5. Using CLUSTAL W and some related softwares, we analyzed the gene structure, protein structure, conserved motifs and phylogenetic relation of genes CCNGl, EEFl A, ETFB-like, HUMMLC2B, PGK1, TXNIP and CKM. In addition, the corresponding phylogenetic trees were constructed.6. Using semi-quantitative RT-PCR with an inner control, we carried out the temporal and spatial expression analyses in different pig breeds (Yorkshire; MeishanxYorkshire; YorkshirexMeishan; LandracexYorkshire; Landrace; Meishan; Duroc; Tongcheng), different development stages (embryo; neonate; two months old; four months old; six months old) and different tissues (heart; liver; spleen; lung; kidney; pancreas; small intestine; stomach; uterus; ovary; testis; adipose tissue; biceps femoris; semispinalls capitis; longissimus dorsi).7. Using PCR-Walking, we obtained 3 genes' full genomic sequences containing all introns and 2 genes' partial genomic sequences, and analyzed their polymorphisms in different pig breeds. (1) CCNGl, 7491 bp, containing 6 introns and 7 exons. In whole genomic sequence, we identified 23 SNPs. Of 4 are transversion mutations, 17 are
transition mutations, 1 is insertion mutation and 1 is deletion mutation. In addition, there is an "AATGAATCTGTGTACTGAATC" insertion in intron 2 of Yangxin pig's CCNG1. (2) HUMMLC2B, 2936 bp, GenBank accession number AY870651, containing 6 introns and 7 exons. In exon 4 to exon 7 (containing partial exon 4 and exon 7), we identified 13 SNPs. Of 4 are transversion mutations, 9 are transition mutations. In addition, there is a GA—TC mutation in intron 4. (3) TXNIP, 3331 bp, containing 7 introns and 8 exons. In whole genomic sequence, we identified 11 SNPs. Of 3 are transversion mutations, 8 are transition mutations. (4) PGK1, 1105 bp (containing partial exon 9 and exon 11, complete intron 9, 10 and exon 10), we identified 8 SNPs. Of 1 is transversion mutations, 7 are transition mutations. (5) KIAA1717, 2032 bp (3' UTR), GenBank accession number AY900164, we identified 3 SNPs. all are transition mutations. The locations of splice donor/acceptor sites in all introns follow the "GT/AG" rule.8. Using PCR-RFLP, we detected 4 SNPs in different pig populations and observed associations with traits in YorkshirexMeishan F2 population. The results showed: MS60-2-iVco I -RFLP is significant association with intramuscular fat (p<0.01) and water moisture (p<0.01). MS60-345-l-IspE I -RFLP is significant association with buttock backfat thickness (p<0.01), shoulder backfat thickness (p<0.05), 6-7th thorax backfat thickness (p<0.05), average backfat thickness (p<0.05) and water moisture (p<0.05). DB245-2-Msp I -RFLP is significant association with loin eye width (p<0.05), loin eye area (p<0.05), longissimus doris pH (p<0.05), biceps femoris pH (p<0.01), drip loss rate (p<0.01), water holding capacity (p<0.01), longissimus doris color value (p<0.01), biceps femoris color value (p<0.05) and water moisture (p<0.01). DB37-Msp I -RFLP is significant association with fat meat percentage (ps. fat meat (p<0.05), thorax-waist backfat thickness (p<0.05), buttock backfat thickness (p<0.05), average backfat thickness (p<0.05), loin eye height (p<0.05), loin eye area (p<0.05), carcass length to 1st spondyle (p<0.01), carcass length to Is' rib (p<0.05), longissimus doris marbling (p<0.05), biceps femoris marbling (p<0.01), intramuscular fat (p<0.01), and water moisture (p<0.01).
引文
1.方美英,赵兴波,李宁,吴常信.不同品种猪解耦联蛋白基因3′调控区的遗传变异.科学通报,2002,47(13):1010-1012
2.王楚端,陈清明.民猪、北京黑猪及长白猪背最长肌还原糖含量测定.养猪,1996a,1:28
3.王楚端,陈清明.长白猪、北京黑猪及民猪肌肉组织学特性研究.中国畜牧杂志,1996b,32(4):33-34
4.王楚端,陈清明.长白猪、北京黑猪及民猪脂肪酸及氨基酸组成。中国畜牧杂志,1996c,32(6):19-20
5.王继英,王怀中,张印.猪肌纤维特性的研究进展.养猪,2004,3:46-48
6.左波.猪3、4和7号染色体OTL定位及六个候选基因的分离、鉴定.[博士学位论文].武汉:华中农业大学图书馆,2004
7.关学敏,郭传甲.利用分子技术改良猪肉品质.畜牧与饲料科学,2004,3:40-42
8.刘全胜,刘建华,金由辛,王德宝.酵母tRNA结合蛋白(43 kD蛋白)羧端cDNA的克隆及其序列测定.中国生物化学与分子生物学报.2002,18(3):361-366
9.刘红林,姜志华,方美英.中国地方猪种生长激素基因HhaI酶切片段多态特征研究.江苏农业学报,1998,14(2):85-89
10.刘红林,陈杰,徐银学,王林云.猪肉品质及其影响因素.畜牧与兽医,2000,32(6):38-40
11.刘桂兰.猪脂肪沉积相关候选基因MC3R,MC4R和IGF2的遗传效应研究.[硕士学位论文].武汉:华中农业大学图书馆,2002
12.孙有平,夏祖灼,甘杰.太湖猪银染核仁组成区的研究.南京农业大学学报,1993,16(1):72-77
13.孙金梅,路兴中,杨公社,唐文花.不同经济类型猪种脂肪细胞的发育及其脂肪合成能力的比较研究.畜牧兽医学报,2000,31(2):131-136
14.孙博兴,侯万文,许志锁,谢忠忱,田家良.选择指数结合应激敏感基因型选择方法初探.养猪,2000,1:31-32
15.朱明显.中、小型民猪和长白猪肉质特性的研究.辽宁畜牧兽医,1989,1:1-4
16.朱正茂,赵书红,陈浩,余梅,刘榜,樊斌,朱猛进,李奎.基于cDNA宏阵列的系统聚类分析猪发育阶段的基因表达谱.武汉大学学报(理学版),2004,50(4):482-486
17.杜立新,柳淑芳,闫艳春,姜运良.猪FSHβ亚基基因结构区Alu序列插入突变的研究.遗传学报,2002,29(11):977-982
18.李凤娥,熊远著,邓昌彦.猪品种间ESR基因PCR-RFLP的初步研究.华中农业大学学报,2000,19(1):37-39
19.李国治,连林生,鲁绍雄.猪肉质性状的评定及其主基因的研究进展.云南畜牧兽医,2004,1:8-10
20.吴桢方.HSL和LPL作为猪脂肪沉积性状候选基因的研究.[博士学位论文].武汉:华中农业大学图书馆,1998
21.吴德,杨凤,周安国,陈代文.含不同比例梅山猪血缘杂交肉猪肉质及肌纤维组织学特性研究.养猪,2003,1:24-27
22.肖璐,李宁,陈永福.猪雌激素受体(ESS)位点RFLP的分析.遗传,1997,19:32-34
23.杨公社,高整团,刘艳芬,路兴中,刘孝惇.八眉猪肌肉品质研究.中国农业科学,1994,27(5):63-68
24.杨仕柳,曾昭顺,吴晓林,彭英林,左剑波.湘黄猪Atrn基因的PCR-RFLP及其与生长和胴体性状间的关系.畜牧兽医学报,2004,35(3):241-245
25.张芸,徐小波,张建生,顾建平.太湖猪与外种猪杂交的后代杂种优势分析.畜禽业,2000, 2:27-28
26.陈克飞,黄路生,李宁,张勤,罗明,吴常信.猪雌激素受体(ESR)基因对产仔数性状的影响.遗传学报,2000,27(10):853-857
27.季海峰.猪不同组织中NAPDH-生成酶活性规律的探讨.山东农业大学学报,1994,25(1):27-31
28.姜运良,李宁,习欠云.猪雌激素受体基因(ESR)点突变的PCR-SSCP检测.遗传,2000,22(4):214-216
29.姜运良,李宁,吴常信,杜立新.不同品种猪肌肉生长抑制素基因单核苷酸多态性分析.遗传学报,2001,28(9):840-845
30.姜运良,李宁,杜立新,吴常信.猪肌肉生长抑制素基因5′调控区T→A突变与生长性状的关系分析.遗传学报,2002,29(5):413-416
31.周俊玲,彭中镇,李奎,赵书红,龚炎长,熊统安,刘平华,陈永久,张亚平,罗明,黄路生.二花脸猪和杜洛克猪的RAPD分析.畜牧兽医学报,1999,29(5):385-391
32.周鹏.引物间同源性和裂口对PCR扩增的影响.生命科学研究,2000,4(1):35-40
33.周杰,赵茹茜,韦习会,夏东,胥清富,陈杰.二花脸和大白猪的脂肪组织中GH-R、IGF-1和IGF-1R基因表达的发育性变化.遗传学报,2003a,30(7):657-662
34.周杰,赵茹茜,韦习会,夏东,胥清富,陈杰.二花脸和大白猪脂肪lepan和下丘脑中Ob-Rb基因表达的发育性变化.中国科学(C辑),2003b,33(6):525-530
35.胡边,孙金海,赵志辉.三个家猪品种和rob易位群染色体分带多态性的比较.遗传,1999,21(5):27-30
36.柳万生,路中兴,王永强.中国地方猪种染色体特性.第四次全国畜禽遗传标记研讨会论文集,广州,1993,73-82
37.柳小春,陈斌,施启顺,彭新社,吴民定.杜洛克、大白、长白猪的生长和肉用性状杂交效果研究.中国畜牧杂志,1999,35(5):14-15
38.晏学明,郭源梅,丁能水,任军,黄路生.不同品种猪α1-岩藻糖转移酶基因遗传变异初析.中国畜牧杂志,2004,40:8-10
39.俞沛初.香猪的特性及PIT1基因多态性的研究.山西农业大学博土研究生毕业论文,2003
40.俞沛初,郭传甲,乔中东,方心葵,龚振明.香猪垂体特异性转录因子基因及其相关基因表达水平的半定量分析.养猪,2004,3:21-23
41.郑梁,金岩,肖明振,轩昆.牙齿形状调控相关基因Wnt6的克隆.临床口腔医学杂志,2004,20(4):212-214
42.高勤学,李俊,刘红林,王林云,徐银学.二花脸猪与大约克猪生长期肌内脂肪合成与水解基因表达特征的比较研究.遗传学报,2004,31(11):1218-1225
43.涂荣剑,邓昌彦,熊远著.猪UCP3基因部分编码区序列分析及其单核苷酸多态与胴体、肉质性状的遗传效应.遗传学报,2004,31(8):807-812
44.徐银学,夏祖灼,甘杰,姜志华.二花脸大约克纯种猪染色体C-带的定量比较.南京农业大学学报,1994,17(2):70-74
45.梁自文,罗向东,杨宗诚.低丰度差异转录基因的克隆和高通量筛选.国外医学遗传学分册,2001,24(3):172-174
46.曾勇庆,樊新忠,王慧,王存芳,张民.杜洛克和汉普夏猪及其正反交F1杂种猪肉质特性的研究.中国畜牧杂志,2001,37(1):20-21
47.熊远著.猪的生化及分子遗传实验导论.北京,中国农业出版社,1999a,39-51
48.熊远著,邓昌彦.种猪测定原理及方法.北京,中国农业出版社,1999b,57-118
49.戴如娟,李宁,吴常信.猪肥胖基因(ob)部分序列的克隆与多态性分析.遗传,1997,19:29-31
50. Acton J C, Ziegler G R, Burge DL Jr. Functionality of muscle constituents in the processing of comminuted meat products. Crit Rev Food Sci Nutr, 1983,18(2): 99-121
51. Adams M D, Kelley J M, Gocayne J D, Dubnick M, Polymeropoulos M H, Xiao H, Merril C R, Wu A, Olde B, Moreno R F. Complementary DNA sequencing: expressed sequence tags and human genome project. Science, 1991, 252(5013): 1651-1656
52. Altschul S F, Madden T L, Schaffer A A, Zhang J, Zhang Z, Miller W, Lipman D J. Gapped BLAST and PSI-BLAST: a new generation of protein database searchprograms. Nucleic Acids Res, 1997,25: 3389-3302
53. Amarger V, Nguyen M, Van Laere A S, Braunschweig M, Nezer C, Georges M, Andersson L. Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs. Mamm Genome, 2002, 13(7): 388-98
54. Andersson L, Haley C S, Ellegren H, Knott S A, Johansson M, Andersson K, Andersson-Eklund L, Edfors-Lilja I, Fredholm M, Hansson 1, et al. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science, 1994,263(5154): 1771-1774
55. Andersson-Eklund L, Marklund L, Lundstrom K, Haley C S, Andersson K, Hansson I, Moller M, Andersson L. Mapping quantitative trait loci for carcass and meat quality traits in a wild boar x Large White intercross. J Anim Sci, 1998,76:694-700
56. Andoh T, Chock P B, Chiueh C C. The roles of thioredoxin in protection against oxidative stress-induced apoptosis in SH-SY5Y cells. J Biol Chem, 2002,277(12): 9655-9660
57. Anton B Y I, Fesus L, and Zsolani A. Simultaneous idendification of two MspI polymorphisms of the porcine myogenin gene in Hungarian breeds. J Anim Breed Genet, 2002,119: 280-283
58. Arnold H H and Winter B. Muscle differentistion: more complexity to the network of myogenic regulation. Curr Opin Genet Dev, 1998,8(5): 539
59. Axford R F E, Bishop S C, Nicholas F W, Owen J B. Breeding for disease resistance in farm animals. 2th edition. CABI Publishing, New York, 2000
60. Bai Q, McGillivray C, da Costa N, Dornan S, Evans G, Stear M J, Chang K C. Development of a porcine skeletal muscle cDNA microarray: analysis of differential transcript expression in phenotypically distinct muscles. BMC Genomics, 2003,4(1): 8
61. Bandman E, Zdanis D. An immunological method to assess protein degradation in postmortem muscle. Meat Sci, 1988,22:1-19
62. Banfi S, Guffanti A, Borsani G How to get the best of dbEST. Trends Genet, 1998,14(2): 80-81
63. Berberich T, Sugawara K, Harada M, Kusano T. Molecular cloning, characterization and expression of an elongation factor 1 alpha gene in maize. Plant Mol Biol, 1995,29(3): 611-615
64. Bertani G R, Gladney C D, Johnson R K, Pomp D. Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR: Ⅱ. Anterior pituitary. J Anim Sci, 2004, 82(1): 32-40.
65. Bessman S P, Carpenter C L. The creatine-creatine phosphate energy shuttle. Annu Rev Biochem, 1985,54: 831-862
66. Blomberg le A, Long E L, Sonstegard T S, Van Tassell C P, Dobrinsky J R, Zuelke K A. Serial analysis of gene expression during elongation of the peri-implantation porcine trophectoderm (conceptus). Physiol Genomics, 2005,20(2):188-94.
67. Breathnach R, Benoist C, O'Hare K, Gannon F, Chambon P. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc Natl Acad Sci USA, 1978,10:4853-4857
68. Bumstead N, Millard B M, Barrow P A, Cook J K A. Genetic Basis disease resistance in chickens. In: Owen J B & Axford R F E (Eds) Breeding for Disease Resistance in Farm Animals. CAB International, Wallingford, 1991,10-23
69. Buonomo F C, Lauterio T J, Baile C A, Campion D R. Determination of insulin-like growth factor 1(1GF1) and IGF binding protein levels in swine. Dottiest Anim Endocrinol, 1987, 4(1): 23-31
70. Chehab F F, Lim M E, Lu R. Correction of the sterility defect in homozygous obese female mice by treatmant with the human recombinant leptin. Nature Genetics, 1996,12: 18-20
71. Chen K F, Beck J, Huang L S, Knorr C, Brenig B. Assignment of the phosphoglycerate kinase 1 ( PGK1) gene to porcine chromosome Xql2-ql3 by fluorescence in situ hybridization and hybrid panel analyses. Anim Genet, 2004, 35(2): 143-145
72. Chen Y, Cai J, Murphy T J, Jones D P. Overexpressed human mitochondrial thioredoxin confers resistance to oxidant-induced apoptosis in human osteosarcoma cells. J Biol Chem, 2002, 277(36): 33242-33248
73. Christian L. Clarifying the impact of the stress gene. Natl Hog Farmer, 1995, 40(6): 44-46
74. Clarke F M, Orozco C, Perkins A V, Cock I, Tonissen K F, Robins A J, Wells J R. Identification of molecules involved in the 'early pregnacy factor' phenomenon. J Reprod Fert, 1991, 93: 525-539
75. Clore A M, Dannenhoffer J M, Larkins B A. EF-1a is associated with a cytoskeletal network surrounding protein bodies in maize endosperm cells. Pant Cell, 1996, 8: 2003-2014
76. Clutter A C, Spicer L J, Woltmann M D, Grimes R W, Hammond J M, Buchanan D S. Plasma growth hormone, insulin-like growth factor I, and insulin-like growth factor binding proteins in pigs with divergent genetic merit for postweaning average daily gain. J Anim Sci, 1995, 73(6): 1776-1783
77. Clutter A C, Sasaki S, Pomp D. Polymerase chain reaction-based polymorphisms in the porcine cholecystokinin (CCK) gene and assignment to chromosome 13. Anim Genet, 1996, 27(5): 369-370
78. Clutter A C, Sasaki S, Pomp D. Rapid communication: the cholecystokinin type-A receptor (CCKAR) gene maps to porcine chromosome 8. J Anim Sci, 1998,76(7): 1983-1984
79. De Vries A G, Timm H H, Wilson E R, Evans G, Keller V, Plastow G S. An effective DNA-marker test for the 'acid meat' problem. EAAP Proc, 1997,3:340
80. Diatchenko L, Lau Y F, Campbell A P, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov E D, Siebert P D.. Suppression subtractive hybridization: a method for generating differentially regulated or tissue specific cDNA probes and libraries. Proc Natl Acad Sci USA, 1996,93: 6025-6030
81. Drogemuller C, Thieven U, Harlizius B. An AvaI and a MspAlI polymorphism at the porcine oestrogen receptor (ESR) gene. Anim Genet, 1997, 28(1): 59
82. Dunn M A, Morris A, Jack P L, Hughes M A. A low-temperature-responsive translation elongation factor 1 alpha from barley (Hordeum vulgare L.). Plant Mol Biol, 1993, 23(1):221-225
83. Edfors-Lilja I, Gustafsson U, Duval-Iflah Y, Ellergren H, Johansson M, Juneja R K, Marklund L, Andersson L. The porcine intestinal receptor for Escherichia coli K88ab, K88ac: regional localization on chromosome 13 and influence of IgG response to the K88 antigen. Anim Genet, 1995, 26(4): 237-242
84. Ellegren H, Fredholm M, Edfors-Lilja I, Wintero A K, Andersson L. Conserved synteny between pig chromosome 8 and human chromosome 4 but rearranged and distorted linkage maps. Genomics, 1993,17(3): 599-603
85. Emnett R S, Grindflek E, Rothschild M F, Moeller S J, Meeker D L, Irvin K M. The effects of porcine peroxisome proliferator-activated receptor gamma (PPARG) in Berkshire, Duroc, Hampshire and Landrace breeds. Plant Anim Genome Ⅷ Conf, CA, 2000, P396
86. Endo Y, Fujita T, Tamura K, Tsuruga H, Nojima H. Structure and chromosomal assignment of the human cyclin G gene. Genomics, 1996, 38(1): 92-95
87. Enerback S, Jadobsson A, Simpson E M, Guerra C. Mice lacking mitochondria] uncoupling protein are colding-sensitive but not obese. Nature, 1997, 287: 90-94
88. Ernst C W, Mendez E A, Robic A, Rothschild M F. Myogenin (MYOG) physically maps to porcine chromosome 9q2.1-q2.6. J Anim Sci, 1998a, 76:328
89. Ernst C W, Robic A, Yerle M, Wang L, Rothschild M F. Mapping of calpastatin and three microsatellites to porcine chromosome 2q2.1-q2.4. Anim Genet, 1998b, 29(3): 212-215
90. Estrade M, Vignon X, Monin G Effect of the RN- Gene on Ultrastructure and Protein Fraction in Pig Muscle. Meat Sci, 1993, 35: 313-319
91. Feddern E, Wegner J, Ender K, Kalm E. Untersuchungen von Muskelstrukturmerkmalen bei Hampshire-Reinzuchttieren und verschieden Kreuzungskombinationen. Arch Tierz, 1995, 38: 43-56.
92. Fields C, Adams M D, White O, Venter J C. How many genes in the human genome? Nat Genet, 1994,7(3): 345-6
93. Fujii H, Yoshida A. Molecular abnormality of phosphoglycerate kinase-Uppsala associated with chronic nonspherocytic hemolytic anemia.Proc Natl Acad Sci U S A, 1980,77(9): 5461-5465
94. Fujii J, Otsu K, Zorzato F, de Leon S, Khanna V K, Weiler J E, O'Brien P J, MacLennan D H. Identification of a mutation in porcine ryanodine receptor associated with malignant hypenherinia. Science, 1991,253:448-451
95. Geffrotin C, Popescu P, Cribiu E, Boscher J, Renard C, Chardon P, Vaiman M. Assignment of MHC in swine to chromosome 7 by in situ hybridisation and serological typing. Ann Genet, 1984, 27,213-219
96. Gerbens F, Rettenberger D, Lenstra J A, Veerkamp J H, te Pas MF. Characterization, chromosomal localization, and genetic variation of porcine heart fatty acid-binding protein gene. Mamm Genome, 1997,8: 328-332
97. Gerbens F, Jansen A, van Erp A J, Harders F, Meuwissen T H, Rettenberger G, Veerkamp J H, te Pas M F. The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs. Mamm Genome, 1998,9(12): 1022-1026
98. Gibbons R A, Sellwood R, Burrows M, Hunter P A. Inheritance of resistance to neonatal E.coli diarrhea in the pig: Examination of the genetic system. Theor Appl Genet, 1977,51:65-70
99. Gladney C D, Bertani G R, Johnson R K, Pomp D. Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR and human microarrays: I. Ovarian follicles. J Anim Sci, 2004, 82(1):17-31
100. Glotzer M, Murray A W, Kirschner M W. Cyclin is degraded by the ubiquitin pathway. Nature, 1991,349:132-138
101. Goll D E, Bray R W, Hoelstra W G. Age-associated changes in bovine muscle connective tissue. III. Rate of solubilization at 100C. J Food Sci, 1964,29:622
102. Goodman S I, Binard R J, Woontner M R, Frerman F E. Glutaric acidemia type Ⅱ: gene structure and mutations of the electron transfer flavoprotein:ubiquinone oxidoreductase (ETF:QO) gene. Mol Genet Metab, 2002, 77(1-2): 86-90
103. Grange P, Vedrine B, Mouricout M. Adhesion of K88ab fimbriated E. coli in piglet small intestines in relation with iron transport molecules. Adv Exp Med Biol, 1997,412: 357-361
104. Green S, Walter P, Kumar V, Krust A, Bornert J M, Argos P, Chambon P. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature, 1986, 320:134-139
105. Green M L, Blaeser L L, Simmen F A, Simmen R C. Molecular cloning of spermidine/spermine N1-acetyltransferase from the periimplantation porcine uterus by messenger ribonucleic acid differential display: temporal and conceptus-modulated gene expression. Endocrinology, 1996, 137(12): 5447-5455
106. Grindflek E, Sundvold H, Lien S, Rothschild M F. Rapid communication: physical and genetic mapping of the Peroxisome Proliferator Activated Receptor gamma (PPAR-gamma) gene to porcine chromosome 13. J Anim Sci, 2000, 78(5): 1391-1392
107. Gu F, Harbitz 1, Chowdhary B P, Bosnes M, Gustavsson I. Chromosomal localization of the hormone sensitive lipase (LIPE) and insulin receptor (INSR) genes in pigs. Hereditas, 1992a, 117(3): 231-236
108. Gu F, Harbitz I, Chowdhary B P, Davies W, Gustavsson I. Mapping of the porcine lipoprotein lipase (LPL) gene to chromosome 14ql2-14 in situ hybridization. Cyto Cell Genet, 1992b, 59: 63-64
109. Habben J E, Moro G L, Hunter B G, Hamaker B R, Larkins B A. Elongation factor 1 alpha concentration is highly correlated with the lysine content of maize endosperm. Proc Natl Acad Sci U S A, 1995,92(19): 8640-8644
110. Harbitz I, Kristensen T, Kran S, Davies W. Isolation and sequencing of porcine lipoprotein lipasecDNA and its use in multiallelic restriction fragment length polymorphism detection. Anim Genet, 1992,23:517-522
111. Hartwell L. Defects in a cell cycle checkpoint may be responsible for the genomic instability of cancer cells, Cell, 1992,71:543-546
112. Hasan L, Neuenschwander S, Stoll P, Stranzinger G, Vogeli P. A complete exon is missing in the L-gulono-gamma-lactone oxidase gene (GULO) causing vitamin C deficiency in the pig: a DNA based test for the diagnosis of the deficient allele. 27th international conference on animal genetics. Animal genomics: syn-thesis of past, present and future directions. Minnesota, USA, 2000
113. Holmgren A. Thioredoxin and glutaredoxin systems. J Biol Chem, 1989,264:13963-13966
114. Horne M C, Goolsby G L, Donaldson K L, Tran D, Neubauer M, Wahl A F. Cyclin G1 and cyclin G2 comprise a new family of cyclins with contrasting tissue-specific and cell cycle-regulated expression. J Biol Chem, 1996, 271(11): 6050-6061
115. Hubank M, Schatz D G: Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucleic Acids Res, 1994, 22: 5640-5648
116. Huszar D, Lynch C A, Fairchild-Huntress V, Dunmore J H, Fang Q, Berkemeier L R, Gu W, Kesterson R A, Boston B A, Cone R D, Smith F J, Campfield L A, Burn P, Lee F. Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell, 1997,88(1): 131-141
117. Imberechts H, De Greve H, Schlicker C, Bouchet H, Pohl P, Charlier G, Bertschinger H, Wild P, Vandekerckhove J, Van Damme J. Characterization of F107 fimbriae of Escherichia coli 107/86, which causes edema disease in pigs, and nucleotide sequence of the F107 major fimbrial subunit gene, fedA. Infect Immun, 1992,60(5): 1963-1971
118. Jackson I J. The mahogany mouse mutation further links between pigmentation, obesity and the
immune system. Trends Genet, 1999,15:429-431
119. Jacquier-Sarlin M R, Polla B S. Dual regulation of heat-shock transcription factor (HSF) activation and DNA-binding activity by H_2O_2: role of thioredoxin. Biochem J. 1996, 318 (Pt 1): 187-193
120. Janss L L G J, Van Arendonk J A M, Brascamp E W. Bayesian statistical analysis for presence of single genes affecting meat quality traits in a crossed pig population. Genetics, 1997, 145: 395-408
121. Janzen M A, Kuhlers D L, Jungst S B, Louis C F. ARPP-16 mRNA is up-regulated in the longissimus muscle of pigs possessing an elevated growth rate.J Anim Sci, 2000,78:1475-1484
122. Jeon J T, Carlbory O, Tornsten A, Giuffra E, Amarger V, Chardon P, Andersson E L, Andersson K, Hansson I, LundstrOm K, Andersson L. A paterally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the 1GF2 locus. Nature Genetics, 1999, 21: 157-158
123. Jiang Zhi-Hua, John P G Genetic polymorphisms in the leptin gene and their association with fatness in four pig breeds. Mamm Cenome, 1999,10:191-193
124. Jonsson J J, Weissman S M. From mutation mapping to phenotype cloning. Proc Natl Acad Sci USA, 1995,92(1): 83-85
125. Junn E, Han S H, 1m J Y, Yang Y, Cho E W, Um H D, Kim D K, Lee K W, Han P L, Rhee S G, Choi 1. Vitamin D3 up-regulated protein 1 mediates oxidative stress via suppressing the thioredoxin function. J Immunol, 2000,164(12): 6287-6295
126. Kamata H, Hirata H. Redox regulation of cellular signalling. Cell Signal, 1999,11(1): 1-14
127. Kastan M B, Onyekwere 0, Sidransky D, Vogelstein B, Craig R W. Participation of p53 protein in the cellular response to DNA damage. Cancer Res, 1991,51:6304-6311
128. Kenzelmann M, Muhlemann K. Substantially enhanced cloning efficiency of SAGE (Serial Analysis of Gene Expression) by adding a heating step to the original protocol. Nucleic Acids Res, 1999,27: 917-918
129. Kim K S, Larsen N, Rothschild M F. Rapid communication: Linkage and physical mapping of the porcine melanocortin-4 receptor (MC4R) gene. J Anim. Sci., 2000a, 78:791-792
130. Kim K S, Larsen N, Short T, Plastow G, Rothschild M F. A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mamm Genome, 2000b, 11(2):131-135
131. Kimura S H, Kataoka T R, Endo Y, Nojima H. Genomic structure and chromosomal localization of mouse cyclin G1 gene. Genomics, 1997,46(3): 483-486
132. Kirkpatrick B W and Huff B M. Detection of insertion polymorphisms in 5' flank and second intron of the porcine growth hormone gene. Anim Genet, 1991, 22(2): 192-193
133. Kirkpatrick B W. Identification of a conserved microsatellite site in the porcine and bovine insulin-like growth factor-I gene 5~1 flank. Anim Genet, 1992,23(6): 543-548
134. Klukowska J, Urbaniak B, Switonski M. High frequency of M307~a mutation at FUT1 locus, causing resistance to oedema disease, in an autochtonous polish pig breed, the zlotnicka spotted. J Anim Breeding Genet, 1999,116(6): 519-524
135. Knoll A, Putnova L, Dvorak J, Rohrer G A, Cepica S. Linkage mapping of an Ava I PCR-RFLP within the porcine uncoupling protein 3 (UCP3) gene. Anim Genet, 2000,31(2): 156-157
136. Kozak M. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev, 1983,47:1-45
137. Kratzsch A, Stricker C, Gmur C, Rieder S, Jorg H, Ossent P, Burgi E, Zimmermann W,
Stranzinger G, Vogeli P. The heredity disease 'congenital progressive ataxia and spastic paresis in pigs' maps to chromosome 3.27th international conference on animal genetics. Animal genomics: synthesis of past, pre-sent and future directions. Minnesota, USA, 2000
138. Kumar S, Tamura K, Jakobsen I B, Nei M. MEGA2: Molecular Evolutionary Genetics Analysis software, Arizona State University, Tempe, Arizona, USA. 2001
139. Lay A J, Jiang X M, Kisker O, Flynn E, Underwood A, Condron R, Hogg P J. Phosphoglycerate kinase acts in turnout angiogenesis as a disulphide reductase. Nature, 2000, 408:869-873
140. Le Roy, Naveau P J, Elsen J M, Sellier P. Evidence for a new major gene influencing meat quality in pigs. Genet Res, 1990, 55:33-40
141. Lee J Y, Hirono I, Aoki T. Cloning and analysis of expression of Mx cDNA in Japanese flounder, Paralichthys olivaceus. Dev Comp Immunol, 2000, 24(4): 407-415
142. Legradi G, Emerson C H, Ahima R S, Flier J S, Lechan R M. Leptin prevents fasting-induced suppression of prothyrotropin-releasing hormone messenger fibonucleic acid in neurons of the hypothalamic paraventricular nucleus. Endocrinology, 19997, 138:2569-2576
143. Lewin B. Gene V. Oxford, UK: Oxford University Press, 1994
144. Li M D, Matteri R L, Macdonald G J, Wise T H, Ford J J. Overexpression of beta-subunit of thyroid-stimulating hormone in Meishan swine identified by differential display. J Anim Sci, 1996, 74(9): 2104-2111
145. Liang P, Pardee A B. Differential display of eukarytic messenger RNA by means of the polymerase chain reaction. Science, 1992, 257:967-971
146. Linville R C, Pomp D, Johnson R K, Rothschild M F. Candidate gene analysis for loci affecting litter size and ovulation rate in swine. J Anim Sci, 2001, 79(1): 60-67.
147. Lisitsyn N, Lisitsyn N, Wigler M: Cloning the differences between two complex genomes. Science, 1993, 259:946-951
148. Liu B H. Statistical Genomics: Linkage, Mapping, and QTL Analysis. CRC Press, LLC, 1998: 404-409
149. Lonergan S M, Huff-Lonergan E, Rowe L J, Kuhlers D L, Jungst S B. Selection for lean growth efficiency in Duroc pigs influences pork quality. J Anim Sci, 2001, 79(8): 2075-2085
150. Long M, Rosenberg C, Gilbert W. Intron phase correlation and the evolution of the intron/exon structures of genes. Proc Natl Acad Sci USA, 1995, 92:12495-12499
151. Lubahn D B, Moyer J S, Golding T S, Couse J F, Korach K S, Smithies O. Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene. Proc Natl Acad Sci USA, 1993, 90(23): 11162-11166
152. Lunney J K, Murrell K D. Immunogenetic analysis of Trichinella spiralis infections in swine. Vet Parasitol, 1988, 29(2-3): 179-193
153. Maak S, Jaesert S, Neumann K, von Lengerken G. Characterization of the porcine CDKN3 gene as a potential candidate for congenital splay leg in piglets. Genet Sel Evol, 2003, 35:S157-165
154. Madden K B, Murrell K D, Lunney J K. Trichinella spiralis: major histocompatibility complex-associated elimination of encysted muscle larvae in swine. Exp Parasitol, 1990, 70(4): 443-451
155. Makino Y, Yoshikawa N, Okamoto K, Hirota K, Yodoi J, Makino I, Tanaka H. Direct association with thioredoxin allows redox regulation of glucocorticoid receptor function. J Biol Chem, 1999, 274(5): 3182-3188
156. Mallard B A, Wilkie B N, Kennedy B W. Influence of major histocompatibility genes on serum hemolytic complement activity in miniature swine. Am J Vet Res, 1989, 50(3): 359-363
157. Mariani P, Lundstrom K, Gustafsson U, Enfalt A C, Juneja R K, Andersson L. A major locus(RN) affecting muscle glycogen content is located on pig chromosome 15. Mamm Genome, 1996, 7: 52-54
158. Matsuda M, Masutani H, Nakamura H, Miyajima S, Yamauchi A, Yonehara S, Uchida A, Irimajiri K, Horiuchi A, Yodoi J. Protective activity of adult T cell leukemia-derived factor (ADF) against tumor necrosis factor-dependent cytotoxicity on U937 cells. J Immunol, 1991, 147(11):3837-3841
159. Matthews J R, Wakasugi N, Virelizier J L, Yodoi J, Hay R T. Thioredoxin regulates the DNA binding activity of NF-kappa B by reduction of a disulphide bond involving cysteine 62. Nucleic Acids Res, 1992, 20(15): 3821-3830
160. Meijerink E, Fries R, Vogeli P, Masabanda J, Wigger G, Stricker C, Neuenschwander S, Bertschinger H U, Stranzinger G. Two alpha(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia coli F18 receptor (ECF18R) loci. Mamm Genome, 1997, 8(10): 736-741
161. Mellink C, Lahbib-Mansais Y, Yerle M, Gellin J. PCR amplification and physical localization of the genes for pig FSHB and LHB. Cytogenet Cell Genet, 1995, 70:224-227
162. Mendez E A, Ernst C W, Rothschild M F. Rapid communication: A Novel DNA polymorphism of the porcine myogenin(MYOG) gene. J Anim Sci, 1997, 75:1984
163. Messer L, Wang L, Yelich J, Pomp D, Geisert R, Rothschild M F. Linkage mapping of the retinoic acid receptor-gamma gene to porcine chromosome 5. Anita Genet, 1996a, 27(3): 175-177
164. Messer L, Wang L, Yelich J, Pomp D, Geisert R, Rothschild M F. Linkage mapping of the retinol-binding protein 4 (RBP4) gene to porcine chromosome 14. Mamm Genome, 1996b, 7(5): 396
165. Messer L, Wang L, Legault C, Rothschild M F. Mapping and investigation of candidate genes for litter size in French Large White pigs. Anim. Genet, 1996c, 27(Suppl. 2): 114
166. Mikawa S, Akita T, Hisamatsu N, Inage Y, Ito Y, Kobayashi E, Kusumoto H, Matsumoto T, Mikami H, Minezawa M, Miyake M, Shimanuki S, Sugiyama C, Uchida Y, Wada Y, Yanai S, Yasue H. A linkage map of 243 DNA markers in an intercross of Gottingen miniature and Meishan pigs. Anim Genet, 1999, 30(6): 407-417. Erratum in:Anim Genet, 2000, 31(1): 80-81.
167. Milan D, Le Roy P, Woloszyn N, Caritez J, Elsen J, Sellier P, Gellin J. The RN locus for meat quality maps to pig chromosome 15. Genet Sel Evo, 1995, 27:195-199
168. Milan D, Jeon J T, Looft C, Amarger V, Robic A, Thelander M, Rogel-Gaillard C, Paul S, lannuccelli N, Rask L, Ronne H, Lundstrom K, Reinsch N, Gellin J, Kalm E, Roy PL, Chardon P, Andersson L. A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle. Science, 1998, 288:1248-1251
169. Minchenko A, Leshchinsky I, Opentanova I, Sang N, Srinivas V, Armstead V, Caro J. Hypoxiainducible factor-1-mediated expression of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) gene. Its possible role in the Warburg effect. J Biol Chem, 2002, 277: 6183-6187
170. Mitsui A, Hirakawa T, Yodoi J. Reactive oxygen-reducing and protein-refolding activities of adult T cell leukemia-derived factor/human thioredoxin. Biochem Biophys Res Commun, 1992, 186(3): 1220-1226
171. Montgomery G W, Crawford A M, Penty J M, Dodds K G, Ede A J, Henry H M, Pierson C A, Lord E A, Galloway S M, Schmack A E, Sise J A, Swarbrick P A, Hanrahan V, Buchanan F C, Hill D F. The ovinc Booroola fecundity gone (FecB) is linked to markers from a region of human chromosome 4q. Nature Genet, 1993, 4:410-414
172. Muller E G. A redox-dependent function of thioredoxin is necessary to sustain a rapid rate of DNA synthesis in yeast. Arch Biochera Biophys, 1995, 318(2): 356-361
173. Nakamura H, Matsuda M, Furuke K, Kitaoka Y, Iwata S, Toda K, Inamoto T, Yamaoka Y, Ozawa K, Yodoi J. Adult T cell leukemia-derived factor/human thioredoxin protects endothelial F-2 cell injury caused by activated neutrophils or hydrogen peroxide. Immunol Lett, 1994, 42(1-2): 75-80
174. Nakamura H, Nakamura K, Yodoi J. Redox regulation of cellular activation. Annu Rev Immunol, 1997, 15:351-369
175. Narayanan R A, Rink A, Beattie C W, Hu W S. Differential gene expression analysis during porcine hepatocyte spheroid formation. Mamm Genome, 2002, 13(9): 515-523
176. Neuenschwander S, Rettenberger G, Meijerink E, Jorg H, Stranzinger G. Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids. Anim Genet, 1996, 27(4): 275-278
177. Nezer C, Moreau L, Brouwers B, Coppieters W, Detilleux J, Haunset R, Karim L, Kvasza A, Leroy P, Georges M. An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs. Nature Genetics, 1999, 21:155-156
178. Nielsen H, Engelbrecht J, Brunak S, yon Heijne G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering, 1997, 10:1-6
179. Nielson V H and Larsen N J. Restriction fragment length polymorphisms at the growth hormone gene in pigs. Anim Genet, 1991, 22(3): 291-294
180. Nielsen V H, Bendixen C, Ambjerg J, Sorensen C M, Jensen H E, Shukri N M, Thomsen B. Abnormal growth plate function in pigs carrying a dominant mutation in type Ⅹ collagen. Mamm Genome, 2000, 11(12): 1087-1092
181. Nishiyama A, Masutani H, Nakamura H, Nishinaka Y, Yodoi J. Redox regulation by thioredoxin and thioredoxin-binding proteins. IUBMB Life, 2001, 52(1-2): 29-33
182. Okamoto K, Beach D. Cyclin G is a transcriptional target of the p53 tumor suppressor protein. EMBOJ, 1994, 13:4816-4822
183. Okamoto K, Kamibayashi C, Serrano M, Prives C, Mumby MC, Beach D. p53-dependent association between cyclin G and the B' subunit of protein phosphatase 2A. Mol Cell Biol, 1996, 16(11): 6593-6602
184. Peelman L J. Genetic investigation of the resistance mechanisms of the pig against diarrhea caused by E. coli. Verh K Acad Geneeskd Belg, 1999, 61(4): 489-515
185. Pfander D, Cramer T, Schipani E, Johnson R S. HIF-1alpha controls extracellular matrix synthesis by epiphyseal chondrocytes. J Cell Sci, 2003, 116(Pt 9): 1819-1826
186. Powell J. Enhanced concatemer cloning-a modification to the SAGE (Serial Analysis of Gene Expression) technique. Nucleic Acids Res, 1998, 26:3445-3446
187. Powis G, Kirkpatrick D L, Angulo M, Baker A. Thioredoxin redox control of cell growth and death and the effects of inhibitors. Chem Biol Interact, 1998, 111-112:23-34
188. Previato L, Parrott C L, Santamarina-Fojo S, Brewer H B Jr. Transcriptional regulation of the human lipoprotein lipase gene in 3T3-L1 adipocytes. J Bio chem, 1991, 266(28): 18958-18963
189. Przytulski T, Porzeczkowska D. Studies on genetic resistance to leptospirosis in pigs. Br Vet J, 1980, 136:25-32
190. Reddy A S N, Day I S, Narasimhulu S B, Safadi F, Reddy V S, Golovkin M, Hamly M J. Isolation and characterization of a novel calmodulin-binding protein from potato. J Biol Chem, 2002, 6: 4206-4214
191. Reiner G, Heinricy L, Muller E, Geldermann H, Dzapo V. Indications of associations of the porcine FOS proto-oncogene with skeletal muscle fibre traits. Anim Genet, 2002, 33(1): 49-55
192. Rettenberger G, Bruch J, Fries R, Archibald A L, Hameister H. Assignment of 19 porcine type Ⅰ loci by somatic cell hybrid analysis detects new regions of conserved synteny between human and pig. Mamm Genome, 1996, 7(4): 275-279
193. Roberts D L, Frerman F E, Kim J J. Three-dimensional structure of human electron transfer flavoprotein to 2.1-A resolution. Proc Natl Acad Sci U S A, 1996, 93(25): 14355-14360
194. Rodriguez C, Martinez-Gonzalez J, Sanchez-Gomez S, Badimon L. LDL downregulates CYP51 in porcine vascular endothelial cells and in the arterial wall through a sterol regulatory element binding protein-2-dependent mechanism. Circ Res, 2001, 88(3):268-274
195. Rohrer G A. Phosphoglycerate kinase 1 (PGK1) maps to Ⅹq1.2 in the porcine genome. J Anim Sci, 1999, 77(2): 497-498
196. Ross J W, Ashworth M D, Hurst A G, Malayer J R, Geisert R D. Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization. Reprod Biol Endocrinol, 2003, 1(1): 23
197. Rothschild M F, Chen H L, Christian L L, Lie W R, Venier L, Cooper M, Briggs C, Warner C M. Breed and swine lymphocyte antigen haplotype differences in agglutination titers following vaccination with B. bronchiseptica. J Anim Sci, 1984, 59(3): 643-649
198. Rothschild M F, Jacobson C, Vaske D A, Tuggle C K, Short T, Sasaki S, Eckardt G R, McLaren D G. A major gene for litter size in swine. Proc 5th WCGALP, 1994, 21:225-228
199. Rothschild M F, Jacobscon C, Vaske D, Tuggle C, Wang L, Short T, Eckardt G, Sasaki S, Vincent A, McLaren D, Southwood O, Steen H V D, Mileham A, Plastow G. The estrogen receptor locus is associated with a major gene influencing litter size in pigs. Proc Natl Acad Sci USA, 1996, 93: 201-205
200. Rothschild M F. Identification of quantitative trait loci and interesting candidate genes in the pig : progress and prospect. 6th WCGAL, 1998, 26:403-409
201. Rothschild M F. Journal Papre No. J-18175 of the Iowa Agriculture and Home Economics Experiment Station. Ames, Project 3215, 1999
202. Rothschild M F, Messer L, Day A, Wales R, Short T, Southwood O, Plastow G. Investigation of the retinal-binding protein 1(RBP4) gene as a candidate gene for increased litter size in pigs. Mamm Genom, 2000, 11:75-77
203. Russo V, Fontanesi L, Davoli R, Nanni Costa L, Cagnazzo M, Buttazzoni L, Virgili R, Yerle M. Investigation of candidate genes for meat quality in dry-cured ham production: the porcine cathepsin B(CTSB) and cystatin B(CSTB)genes. Anim Genet, 2001, 33:123-131
204. Sambtook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, 1989.
205. Schallreuter K U, Wood J M. Thioredoxin reductase-its role in epidermal redox status. J Photochem Photobiol B, 2001, 64:179-184
206. Schenk H, Klein M, Erdbrugger W, Droge W, Schulze-Osthoff K. Distinct effects of thioredoxin and antioxidants on the activation of transcription factors NF-kappa B and AP-1. Proc Natl Acad Sci U S A, 1994, 91(5): 1672-1676
207. Scherer G, Telford J, Baldari C, Pirotta V. Isolation of cloned genes differentially expressed at early and late stages of Drosophila embryonic development. Dev Biol, 1981, 86:438-447
208. Schulze P C, De Keulenaer G W, Yoshioka J, Kassik K A, Lee R T. Vitamin D3-upregulated protein-1 (VDUP-1) regulates redox-dependent vascular smooth muscle cell proliferation through interaction with thioredoxin. Circ Res, 2002, 91(8): 689-695
209. Sellwood R, Gibbons R A, Jones G W, Rutter J M. Adhesion of enteropathogenic Escherichia coli to pig intestinal brush borders: the existence of two pig phenotypes. J Med Microbiol, 1975, 8(3): 405-411
210. Shaw G, Kamen R. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell, 1986, 46:659-667
211. Shiina N, Gotoh Y, Kubomura N, Iwamatsu A, Nishida E. Microtubule severing by elongation factor 1 alpha. Science, 1994, 266(5183): 282-285
212. Short T H, Rothschild M F, Southwood O I, McLaren D G, de Vries A, van tier Steen H, Eckardt G R, Tuggle C K, Helm J, Vaske D A, Mileham A J, Plastow G S. Effect of the estrogen receptor locus on reproduction and production traits in four commercial pig lines. J Anim Sci, 1997, 75(12): 3138-3142
213. Smith T P, Rohrer G A, Alexander L J, Troyer D L, Kirby-Dobbels K R, Janzen M A, Comwell D L, Louis C F, Schook L B, Beattie C W. Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that the SLA spans the centromere. Genome Res, 1995, 5(3): 259-271
214. Sonstegard T S, Rohrer G A, Smith T P. Myostatin maps to porcine chromosome 15 by linkage and physical analyses. Anim Genet, 1998, 29(1): 19-22
215. Soren H. Investigation of polymorphisms at gene in the growth hormone axis in cattle and pigs. Proc 4th World Congr Genet Appl Livest Prod, 1990, ⅩⅢ: 151-154
216. Sorthwood O I, Short T H, Plastow G S. Genetic markers for litter size in commercial lines of pig. Proc 6th WCGALP, 1998, 26:453-456
217. Soumillion A, Erkens J H F, Lenstra J A, Rettenberger G, Te Pas M F W. Genetic variation in the porcine myogenin gene locus. Mammalian Genome, 1997, 8:564-568
218. Stanko R T, Ferguson T L, Newman C W, Newman R K. Reduction of carcass fat in swine with dietary addition of dihydroxyacetone and pyruvate. J Anim Sci, 1989, 67(5):1272-1278
219. Stockdale F E, Nikovits W Jr, Christ B. Molecular and cellular biology of avian somite development. Dev Dyn, 2000, 219(3): 304-321
220. Sun H S, Wang L, Rothschild M F, Tuggle C K. Mapping of the natural resistance-associated macrophage protein 1 (1NRAMP1) gene to pig chromosome 15. Anim Genet, 1998, 29(2):138-140
221. Sweeney H L, Bowman B F, Stull J T. Myosin light chain phosphorylation in vertebrate striated muscle: regulation and function. Am J Physiol, 1993, 264:C1085-C1095
222. Tamura K, Kanaoka Y, Jinno S, Nagata A, Ogiso Y, Shimizu K, Hayakawa T, Nojima H, Okayama H. Cyclin G: A new mammalian cyclin with a potential tyrosine phosphorylation site. Oncogene, 1993, 8:2113-2118
223. Tanaka T, Nakamura H, Nishiyama A, Hosoi F, Masutani H, Wada H, Yodoi J. Redox regulation by thioredoxin superfamily; protection against oxidative stress and aging. Free Radic Res, 2001, 33(6): 851-855
224. Tanaka T, Hosoi F, Yamaguchi-Iwai Y, Nakamura H, Masutani H, Ueda S, Nishiyama A, Takeda S, Wada H, Spyrou G, Yodoi J. Thioredoxin-2 (TRX-2) is an essential gene regulating mitochondria-dependent apoptosis. EMBOJ, 2002, 21(7): 1695-1703
225. Thompson J D, Higgins D G, Gibson T J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res, 1994,22:4673-80
226. Tissot R G, Beattie C W, Amoss M S Jr. The swine leucocyte antigen (SLA) complex and Sinclair swine cutaneous malignant melanoma. Anim Genet, 1989,20(1): 51-57
227. Tonin P, Shanske S, Miranda A F, Brownell A K, Wyse J P, Tsujino S, DiMauro S. Phosphoglycerate kinase deficiency: biochemical and molecular genetic studies in a new myopathic variant (PGK Alberta). Neurology, 1993,43(2): 387-391
228. Toogood H S, van Thiel A, Basran J, Sutcliffe M J, Scrutton N S, Leys D. Extensive domain motion and electron transfer in the human electron transferring flavoprotein.medium chain Acyl-CoA dehydrogenase complex. J Biol Chem, 2004,279(31): 32904-32912
229. Towler E M, Wilson L K, Zhou Y C, Ma T S, Fisher R J. A complete system for identifying inhibitors of creatine kinase B.Anal Biochem, 2000,279(1): 96-99
230. Trybus K M.The role of myosin light chains. J Muscle Res Cell Motil, 1994,15:587-594
231. Ueno M, Masutani H, Arai RJ, Yamauchi A, Hirota K, Sakai T, Inamoto T, Yamaoka Y, Yodoi J, Nikaido T. Thioredoxin-dependent redox regulation of p53-mediated p21 activation. J Biol Chem, 1999,274(50): 35809-35815
232. Vale R D, Milligan R A.The way things move: looking under the hood of molecular motor proteins.Science, 2000,288:88-95
233. Van der Steen H, Southwood O, Vries A, Short T, McLaren D, Wei M, Plastow G Evidence of a new genetic marker for litter size in Meishan synthetic pigs. In: Proceedings of International Conference on Animal Biotechnology, 1997, Beijing, China: 17-20
234. Van Laere A S, Nguyen M, Braunschweig M, Nezer C, Collette C, Moreau L, Archibald A L, Haley C S, Buys N, Tally M, Andersson G, Georges M, Andersson L. A regulatory mutation in IGF2 causes a major QTL effect on miscle growth in the pig. Nature, 2003,425(6960): 832-836
235. Varley M A, and Cole D J A. Studies in sow reproduction: 5. The effect of lactation length of the sow on the subsequent embryonic development. Anim. Prod, 1976,22:79-85
236. Velculescu V E, Zhang L, Vogelstein B, Kinzlet K W. Serial analysis of gene expression. Science, 1995,270:484-487
237. Vincent A L, Wang L, Tuggle C K, Robic A, Rothschild M F. Prolactin receptor maps to pig chromosome 16. Mamm Genome, 1997,8(10): 793-794
238. Vincent A L, Short T H, Eckardt G R, Mclaren D G, Southwood O I, Plastow G S, Tuggle C K, Rothschild M F. The prolactin receptor gene is associated with increased litter size in pigs. Proc 6~(th) WCGALP, 1998,27:15-18
239. Vogeli P, Bertschinger H U, Stamm M, Stricker C, Hagger C, Fries R, Rapacz J, Stranzinger G Genes specifying receptors for F18 fimbriated Escherichia coli, causing oedema disease and postweaning diarrhoea in pigs, map to chromosome 6. Anim Genet, 1996,27(5): 321-328
240. Vogeli P, Meijerink E, Fries R, Neuenschwander S, Vorlander N, Stranzinger G, Bertschinger H U. A molecular test for the detection of E. coli F18 receptors: a breakthrough in the struggle against edema disease and post-weaning diarrhea in swine. Schweiz Arch Tierheilkd, 1997,11:479-484
241. von Stein O D, Thies W G, Hofmann M. A high throughput screening for rarely transcribed differentially expressed genes. Nucleic Acids Res, 1997,25:2598-2602
242. Wallimann T, Wyss M, Brdiczka D, Nicolay K, Eppenberger H M. Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy
demands: the 'phosphocreatine circuit' for cellular energy homeostasis. Biochem J, 1992, 281: 21-40
243. Wang H, Cao R, Xia L, Erdjument-Bromage H, Borchers C, Tempst P, Zhang Y. Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase. Mol Cell, 2001, 8: 1207-1217
244. Wang S M and Rowley J D. A strategy for genome-wide gene analysis: Integrated procedure for gene identification. Proc. Natl Acad. Sci. USA, 1998,95:11909-11914
245. Wang Y, De Keulenaer G W, Lee R T. Vitamin D(3)-up-regulated protein-1 is a stress-responsive gene that regulates cardiomyocyte viability through interaction with thioredoxin. J Biol Chem, 2002,277(29): 26496-26500
246. Webb A J. Future challenges in pig genetics. Pig News Inf, 1996, 17(1): 11-16
247. Werner P, Neuenschw ander S, Stranzinger G Characterization of the porcine uncoupling proteins 2 and 3 (UCP2 & UCP3) and their localization to chromosome 9 by somatic cell hybrids. Anim Genet, 1999, 30(3): 221-224
248. Whitworth K M, Agca C, Kim J G, Patel R V, Springer G K, Bivens N, Forrester L J, Mathialagan N, Green J A, Prather R S. Transcriptional Profiling of Pig Embryogenesis by Using a 15K Member Unigene Set Specific for Pig Reproductive Tissues and Embryos. Biol Reprod, 2005, [Epub ahead of print]
249. Wilson J R, Jing C, Walker P A, Martin S R, Howell S A, Blackburn G M, Gamblin S J, Xiao B. Crystal structure and functional analysis of the histone methyltransferase SET7/9. Cell, 2002, 111: 105-115
250. Wilson M E, Sonstegard T S, Smith T P, Fahrenkrug S C, Ford S P. Differential gene expression during elongation in the preimplantation pig embryo. Genesis, 2000, 26(1): 9-14
251. Wintero A K, Fredholm M, Andersson L. Assignment of the gene for insulin-like growth factor-l(IGF-l) to chromosome 5 by linkage mapping. Anim Genet, 1994,25(1):37-39
252. Yerle M. Location of the porcine hormone gene to chromosome 12p~(1.2)-p~(1.5). Anim Genet, 1993, 24(2): 129-131
253. Yokomise H, Fukuse T, Hirata T, Ohkubo K, Go T, Muro K, Yagi K, Inui K, Hitomi S, Mitsui A, et al. Effect of recombinant human adult T cell leukemia-derived factor on rat lung reperfusion injury. Respiration, 1994, 61(2): 99-104
254. Zhao S H, Nettleton D, Liu W, Fitzsimmons C, Ernst C W, Raney N E, Tuggle C K. Complementary DNA macroarray analyses of differential gene expression in porcine fetal and postnatal muscle. J Anim Sci, 2003,81(9):2179-2188
255. Zhao Yao-feng, Li Ning, WU Chang-xin. FSH subunit gene is associated with a major gene controlling litter size in commercial pig breeds. Science in China (Series C), 1998,41(6): 664-668
256. Zhu J K, Damsz B, Kononowicz A K, Bressan R A, Hasegawa P M. A higher plant extracellular vitronectin-like adhesion protein is related to the translational elongation factor-1 alpha. Plant Cell, 1994,6(3): 393-404