针刺对阿霉素致大鼠心肌损伤和骨髓抑制的保护作用
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摘要
目的:观察针刺对阿霉素(adriamycin ADM)所致大鼠心肌损伤和骨髓抑制的保护作用。
方法: 45只SD大鼠随机分为4组:空白对照组10只,ADM模型组15只,针刺预防组10只,针刺治疗组10只。空白对照组大鼠腹腔注射生理盐水模型对照组,针刺预防组,针刺治疗组大鼠隔天腹腔注射阿霉素1.5mg·kg-1,共6次,11天。针刺预防组在造模期间同时给予针刺治疗直到实验结束;针刺治疗组在造模结束后给予针刺7天。第19天测定大鼠心电图,计算死亡率,心脏系数;检测心肌酶:乳酸脱氢酶(LDH)、谷草转氨酶(AST)、肌酸激酶(CK )的活性,检测肌酸激酶同工酶( CK-MB)值;观测心肌病理的改变。计算白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白测定(Hb)、血小板计数(PLT),骨髓有核细胞计数(BMNC)等指标。
结果:
1对心肌损伤的影响:
1.1与ADM模型组比较,针刺预防组和针刺治疗组均可显著改善ADM所致的心功能损伤,减少死亡率,减少CK、CK-MB、LDH、AST的释放(P<0.01或P<0.05)。
1.2光镜下观察,ADM组心肌纤维排列紊乱、肿胀、部分断裂,甚至出现片状坏死,周围有炎性细胞浸润,红细胞漏出明显。针刺预防组,针刺治疗组的损伤明显减轻。
2对骨髓抑制的影响:与ADM模型组比较,针刺预防组大鼠外周血中WBC,PLT及BMNC增高(P<0.01或P<0.05);针刺治疗组的PLT,BMNC水平明显升高具有显著性差异(P<0.05)。其他检测指标与ADM模型组相比有上升趋势,但无统计学意义。
结论:
1针刺对ADM大鼠的心肌功能与结构均有明显的保护作用。针刺能减少大鼠死亡率,减少心肌酶水平,减轻心肌病理改变。
2针刺可改善外周血象,促进骨髓细胞增殖,对经ADM损伤后大鼠的骨髓造血系统有较好的保护作用。
Objective: To observe the protective effect of electroacupuncture (EA) on myocardium injury and bone marrow suppression induced by adriamycin (ADM) in rats.
Methods: 45 SD rats were equally divided into 4 groups:normal control group (NC) with 10 rats, ADM model group (ADM) with 15 rats, electroacupuncture prevention group (EAP) with 10 rats, electroacupuncture treatment group (EAT) with 10 rats. The normal control group was given normal saline by intraperitoneal injection, while other three groups were given ADM at a single dose of 1.5mg·kg-1 by intraperitoneal injection every other day, six times for eleven days.On the nineteenth day, the indexes, such as electrocardiogram (ECG), death rate, heart coefficient and the myocardial enzyme :lactate dehydrogenase (LDH), aspartate aminotransferase (AST), creatine kinase (CK) and creatine kinase isoenzyme MB (CK-MB) were detected. The pathological lesion of myocardium was observed. White blood cell count (WBC), red blood cell count (RBC), hemoglobin measurement (Hb), platelet count (PLT), bone marrow nuclear cell count (BMNC) were detected in rats.
Results:
1 The effects on the myocardium injury:
1.1 Electroacupuncture prevention group and electroacupuncture treatment group could recover cardiac function, reduced the death rate and reduced the release of CK, CK-MB, LDH, AST (P<0.01 or P<0.05) when compared with the ADM model group.
1.2 Myocardial fibers were in disorder condition under optical microscope in ADM model group, in which cellular edema, break or necrosis, infiltration of inflammatory cells, or obvious leakage of red blood cells could be observed. The above injuries could be significantly alleviated in electroacupuncture prevention group and electroacupuncture treatment group.
2 The effects on bone marrow suppression:
The electroacupuncture prevention group could increased significantly the contents of WBC, PLT and BMNC (P<0.01 or P<0.05); electroacupuncture treatment group could increased significantly the contents of PLT, BMNC (P<0.05) when compared with the ADM model group.
Conclusion:
1 EA can protect the heart function and myocardial cellular structure against ADM. EA can reduce the death rate, reduce the release of myocardial enzyme and relieve the pathological lesion of myocardium.
2 EA has a better protective effect on bone marrow suppression induced by ADM in rats, which can improve the peripheral blood, and promote the proliferation of bone marrow cells.
方法: 45只SD大鼠随机分为4组:空白对照组10只,ADM模型组15只,针刺预防组10只,针刺治疗组10只。空白对照组大鼠腹腔注射生理盐水模型对照组,针刺预防组,针刺治疗组大鼠隔天腹腔注射阿霉素1.5mg·kg-1,共6次,11天。针刺预防组在造模期间同时给予针刺治疗直到实验结束;针刺治疗组在造模结束后给予针刺7天。第19天测定大鼠心电图,计算死亡率,心脏系数;检测心肌酶:乳酸脱氢酶(LDH)、谷草转氨酶(AST)、肌酸激酶(CK )的活性,检测肌酸激酶同工酶( CK-MB)值;观测心肌病理的改变。计算白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白测定(Hb)、血小板计数(PLT),骨髓有核细胞计数(BMNC)等指标。
结果:
1对心肌损伤的影响:
1.1与ADM模型组比较,针刺预防组和针刺治疗组均可显著改善ADM所致的心功能损伤,减少死亡率,减少CK、CK-MB、LDH、AST的释放(P<0.01或P<0.05)。
1.2光镜下观察,ADM组心肌纤维排列紊乱、肿胀、部分断裂,甚至出现片状坏死,周围有炎性细胞浸润,红细胞漏出明显。针刺预防组,针刺治疗组的损伤明显减轻。
2对骨髓抑制的影响:与ADM模型组比较,针刺预防组大鼠外周血中WBC,PLT及BMNC增高(P<0.01或P<0.05);针刺治疗组的PLT,BMNC水平明显升高具有显著性差异(P<0.05)。其他检测指标与ADM模型组相比有上升趋势,但无统计学意义。
结论:
1针刺对ADM大鼠的心肌功能与结构均有明显的保护作用。针刺能减少大鼠死亡率,减少心肌酶水平,减轻心肌病理改变。
2针刺可改善外周血象,促进骨髓细胞增殖,对经ADM损伤后大鼠的骨髓造血系统有较好的保护作用。
Objective: To observe the protective effect of electroacupuncture (EA) on myocardium injury and bone marrow suppression induced by adriamycin (ADM) in rats.
Methods: 45 SD rats were equally divided into 4 groups:normal control group (NC) with 10 rats, ADM model group (ADM) with 15 rats, electroacupuncture prevention group (EAP) with 10 rats, electroacupuncture treatment group (EAT) with 10 rats. The normal control group was given normal saline by intraperitoneal injection, while other three groups were given ADM at a single dose of 1.5mg·kg-1 by intraperitoneal injection every other day, six times for eleven days.On the nineteenth day, the indexes, such as electrocardiogram (ECG), death rate, heart coefficient and the myocardial enzyme :lactate dehydrogenase (LDH), aspartate aminotransferase (AST), creatine kinase (CK) and creatine kinase isoenzyme MB (CK-MB) were detected. The pathological lesion of myocardium was observed. White blood cell count (WBC), red blood cell count (RBC), hemoglobin measurement (Hb), platelet count (PLT), bone marrow nuclear cell count (BMNC) were detected in rats.
Results:
1 The effects on the myocardium injury:
1.1 Electroacupuncture prevention group and electroacupuncture treatment group could recover cardiac function, reduced the death rate and reduced the release of CK, CK-MB, LDH, AST (P<0.01 or P<0.05) when compared with the ADM model group.
1.2 Myocardial fibers were in disorder condition under optical microscope in ADM model group, in which cellular edema, break or necrosis, infiltration of inflammatory cells, or obvious leakage of red blood cells could be observed. The above injuries could be significantly alleviated in electroacupuncture prevention group and electroacupuncture treatment group.
2 The effects on bone marrow suppression:
The electroacupuncture prevention group could increased significantly the contents of WBC, PLT and BMNC (P<0.01 or P<0.05); electroacupuncture treatment group could increased significantly the contents of PLT, BMNC (P<0.05) when compared with the ADM model group.
Conclusion:
1 EA can protect the heart function and myocardial cellular structure against ADM. EA can reduce the death rate, reduce the release of myocardial enzyme and relieve the pathological lesion of myocardium.
2 EA has a better protective effect on bone marrow suppression induced by ADM in rats, which can improve the peripheral blood, and promote the proliferation of bone marrow cells.
引文
[1]Thomas L,Bellmont S,Christen MO,et al.Cardiovascular and survival effects of sympatho-inhibitors in adriamycin-induced cardiomyopathy in rats[J] .Fundam Clin Pharmacol,2004,18(6): 649.
[2]徐萌,张积仁,许少珍.阿霉素心脏毒性发生的机制极其防治[J] .第一军医大学学报,2001,21(7):532-534.
[3]Tokarska-Schlattner M, Wallimann T, Schlattner U. Al-terations in myocardial energy metabolism induced by the anti-cancer drug doxorubicin[J]. Comptes Rends Biologics 2006,329(9): 657-668.
[4]Foreshow JH. Doxorubicin induced cardiac toxicity[J].N Eng J Med,1991,324(12): 843-845.
[5]Moroak S, Miura T. Free radicals mediate cardiac toxicity induced by adriamycin[J].Yakugaku Zasshi,2003,123(10): 855-866.
[6]Wallace KB. Doxorubicin induced cardiac itochon drionopathy[J]. Pharmacology Texaco, 2003, 93(3): 105-115.
[7]华兴邦.大鼠穴位图谱的研制[J].实验动物与动物实验.1991,(1): 1-3.
[8]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003, 327-328.
[9]Rona G,ChappelL CI,Balazs T,et al.An infarct-like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat.AMA Arch Pathol,1959,67(4):443-455.
[10]李莹.玉郎伞多糖对环磷酰胺所致骨髓抑制的保护作用研究. [学位论文],南宁,广西医科大学,2009.
[11]施献猷.医学动物实验方法[M].北京:人民卫生出版社1980.228,269,279.
[12]徐淑云主编.临床药理学[M].第3版,人民卫生出版社,2006: 447-458.
[13]杨宝峰主编.药理学[M].第7版,人民卫生出版社,2008;457-469.
[14]Rohrdanz E,Obertrifter B,Ohler S,et al. Influence of Adriamycin and paraquat on antioxidant enzyme expression in primary rat hepatocytes [J].Arch Toxicol,2000,74(4-5):231.
[15]Mukherjee S , Banerjee SK , Maulik M . Protection against acuteadriamycin-inducedcardiotoxicity by garlic : role of endogenous antioxidants and inhibitionof TNF - alpha expression[J] .BMPharmacol, 2003,3(1):16.
[16]王震虹,王祥瑞.针刺对缺血心肌保护作用机制研究[J].医学报告,2003,11:28-29.
[17]Minotti G,Menna P,Salvatorelli E,et a1.Anthracyclines:molecular advances and pharmacologic developments in antitumor activity and cardiotoxicity [J] .Pharmacol Rev,2004, 56: 185.
[18]李世红,王绍军.阿霉素心脏毒性发病机制性进展[J].临床心血管病杂志,2005,21(4):249-250.
[19] Deres P,Halmosi R,Toth A,et a1.Prevention of doxorubicin -in duced acute cardiotoxicity by an experimental antioxidant compound[J].J Cardiovasc Phannacol,2005,45(13);36.
[20]黄志煜,范方,刘鲁迎,等.阿霉素心脏毒性研究进展[J] .中国误诊学杂志,2003,3(4):521-522.
[21]陈玲.蒽环类化疗药物心脏毒性的监护[J].国外医学内科分册, 2000,27(12):525-528.
[22]Goormaghtigh E,Huart P,P m e t M,et a1.Structure of the adr iamycin-cardiolipin complex:role in mitochondrial toxicity[J].Biophys Chem,1990,35:247.
[23]Calderone A,Dechamplain J,Rouleau JL.Adriamycin induced changes tothe myocardial beta-adrenergic system in the rabbit [J].J Med Cell Cardiol,1994,23:333.
[24]薛茂强,高音,李霄凌,高喜仁.温针对异丙肾上腺素所致的大鼠急性心肌损伤保护的组化观察[J].齐齐哈尔医学院学报,1998, 19(1): 1-3.
[25]谷万里,史载祥.中药保护心肌缺血损伤的非循环机制研究进展[J].中西医结合心脑血管病杂志,2005, 3(5): 422-424.
[26]姜桂美,于华荣,王艳丽.针灸防治恶性肿瘤放化疗所致骨髓抑制的研究进展[J].江苏中医,2000,21(6):45-46.
[27]崔瑾,申定珠,熊芳丽.针刺、艾灸膈俞穴对低白细胞模型大鼠白细胞及骨髓造血功能的调节作用[J].上海针灸杂志,2005,6,24(6):41-43.
[28]郁美娟,杨金洪,陈桂萍,等.针刺治疗放化疗所致造血功能损害的临床观察[J].中国针灸, 1994, 14(5): 4-6.
[29]李晔,于耀才,戴铁成.针刺治疗恶性肿瘤放化疗副反应的临床研究[J].中国针灸,1997,(6):327-328.
[1]Macrides TA,Naylor LM,Kalafatis N,etal. Hepatoprotective effects of the shark bile salt 5 betascymnolon acetaminoph-induced liver damage in mice[J]. Fundam Appl Toxicol,1996,33:31-37.
[2]Sarieh TC , Zhou T , Adams SP . A model of isoniazid induced hepatotoxicity in rabbits[J]. Pharmacol Toxicol Methods,1995,34(2):109-116.
[3]创斌,淤泽溥,林青,等.去脂软肝丸对四环素致小鼠脂肪肝模型肝脂的影响[J].云南中医学院学报,2002,25(1):8-10.
[4]林京玉,丁日高.抗菌药和药物性肝损伤[J].国外医学·药学分册,2006 33(3):195-197.
[5]孙成春,郝俊文,尹秋霞,等.汉防己甲素对环孢素A致小鼠肝肾损伤的作用[J].中国中药杂志,1998,23(6):373-374.
[6]李钦民,韩真.茶多酚对雷公藤内酯醇致小鼠肝损害的保护用[J].世界华人消化杂志,2006,14(9):908-911.
[7]唐云安,刘玉清,王国钦.肝损伤动物模型研究进展[J].卫生毒理学杂志,2002,16﹝4﹞:236-238.
[8]Berger ML,Bhatt H ,Combes B ,et al. CC14-induced toxicity in isolated hepatocytes[J]. Hepatology,1986,6:36-45.
[9]Decker K,Keppler D.Galactosamine induced liver injury[J]. Prog Liver Dis,1972,4:183-199.
[10]Bruck R,Shirin H ,Aeed H et al.Prevention of hepaticcirrhosis in rats by hydroxyl radical scavengers[J]. Hepatol,2001,35(3):457-464.
[11]王宪波,刘平,陆雄,等.二甲基亚硝胺大鼠肝纤维化形成中门脉压力的动态变化[J].世界华人消化杂志,2002,10(4) :401-405
[12] Ishigami M,Nishimura H ,Naiki Y ,et a1.The roles of intrahepatic valphal4(+)NK1.1(+)T cells for liver injury induced by salmonella infection in mice[J].Hepatology,1999,29(6):1799-1808.
[13]Jarvelainen HA,Fang C,Ingelman SM ,et a1.Effect of chronic coadministration of endotoxin and , ethanol on rat liver pathology and proinflammatory and antiinflammato-ry eytokines[J].Hepatology,1999,29(5):1503-1510.
[14]赵健雄,吴红梅,白德成,等.大鼠镍染毒致肝细胞部分离子变化及中药的治疗作用[J].中国职业医学,2004,31(6):31-33.
[15]孙应彪,朱玉真.镍铬钴混合物对小鼠肝脏毒作用机制的研究[J].中国公共卫生,2003,19(2):161-162.
[16]刘起展,董国宾,崔瑞平,等.刺梨汁饮料对锰中毒脂质过氧化损害的拮抗作用[J].卫生毒理学杂志,1999,13(1):42-44.
[17]于淑兰,邓一夫,王悦,等.锰、铅联合染毒对小鼠肝内部分生化指标的影响[J].卫生毒理学杂志,2000,14(4):228-229.
[18]陈永康,针灸足三里HbaAg携带者免疫功能影响的研究[J].中国针灸,1993,13(2):33-35.
[19]姚俊青,诚杰,笃一,针灸对大白鼠实验性肝损伤影响[J].上海针灸杂志,1995,14(5):234.
[20]周冬松,张利.电针内关、足三里为主治疗呃逆42例[J].中国针灸,2002,22(7):470.
[21]杨金洪.针刺防治环磷酰胺所致大鼠肝肾功能及脏器损害的作用[J].中国针灸,l99B.13(4):32-33.
[22]朱兆洪,丁柱,汤希盂,等.针刺“足三里”对脾虚小鼠脑组织中NO和NOS影响的实验研究[J].中国针灸,2000,20(5):309-311.
[23]马振亚.针刺对正常动物IL-2.IFN.NKC免疫调节网的影响[J].西安医科大学学报,1991,12(2):ll6-1l9.
[24]孙世晓,曾艳,张韵娴.针刺“曲泉”穴对兔胆汁分泌影响观察[J].针灸临床杂志,2001,l7(1):49.
[25]张茹华,周建华,王翰如,等.电针阳陵泉穴对兔胆囊压力变化的影响[J].山东中医学院学报,l995,19(3):197-198.
[2]徐萌,张积仁,许少珍.阿霉素心脏毒性发生的机制极其防治[J] .第一军医大学学报,2001,21(7):532-534.
[3]Tokarska-Schlattner M, Wallimann T, Schlattner U. Al-terations in myocardial energy metabolism induced by the anti-cancer drug doxorubicin[J]. Comptes Rends Biologics 2006,329(9): 657-668.
[4]Foreshow JH. Doxorubicin induced cardiac toxicity[J].N Eng J Med,1991,324(12): 843-845.
[5]Moroak S, Miura T. Free radicals mediate cardiac toxicity induced by adriamycin[J].Yakugaku Zasshi,2003,123(10): 855-866.
[6]Wallace KB. Doxorubicin induced cardiac itochon drionopathy[J]. Pharmacology Texaco, 2003, 93(3): 105-115.
[7]华兴邦.大鼠穴位图谱的研制[J].实验动物与动物实验.1991,(1): 1-3.
[8]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003, 327-328.
[9]Rona G,ChappelL CI,Balazs T,et al.An infarct-like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat.AMA Arch Pathol,1959,67(4):443-455.
[10]李莹.玉郎伞多糖对环磷酰胺所致骨髓抑制的保护作用研究. [学位论文],南宁,广西医科大学,2009.
[11]施献猷.医学动物实验方法[M].北京:人民卫生出版社1980.228,269,279.
[12]徐淑云主编.临床药理学[M].第3版,人民卫生出版社,2006: 447-458.
[13]杨宝峰主编.药理学[M].第7版,人民卫生出版社,2008;457-469.
[14]Rohrdanz E,Obertrifter B,Ohler S,et al. Influence of Adriamycin and paraquat on antioxidant enzyme expression in primary rat hepatocytes [J].Arch Toxicol,2000,74(4-5):231.
[15]Mukherjee S , Banerjee SK , Maulik M . Protection against acuteadriamycin-inducedcardiotoxicity by garlic : role of endogenous antioxidants and inhibitionof TNF - alpha expression[J] .BMPharmacol, 2003,3(1):16.
[16]王震虹,王祥瑞.针刺对缺血心肌保护作用机制研究[J].医学报告,2003,11:28-29.
[17]Minotti G,Menna P,Salvatorelli E,et a1.Anthracyclines:molecular advances and pharmacologic developments in antitumor activity and cardiotoxicity [J] .Pharmacol Rev,2004, 56: 185.
[18]李世红,王绍军.阿霉素心脏毒性发病机制性进展[J].临床心血管病杂志,2005,21(4):249-250.
[19] Deres P,Halmosi R,Toth A,et a1.Prevention of doxorubicin -in duced acute cardiotoxicity by an experimental antioxidant compound[J].J Cardiovasc Phannacol,2005,45(13);36.
[20]黄志煜,范方,刘鲁迎,等.阿霉素心脏毒性研究进展[J] .中国误诊学杂志,2003,3(4):521-522.
[21]陈玲.蒽环类化疗药物心脏毒性的监护[J].国外医学内科分册, 2000,27(12):525-528.
[22]Goormaghtigh E,Huart P,P m e t M,et a1.Structure of the adr iamycin-cardiolipin complex:role in mitochondrial toxicity[J].Biophys Chem,1990,35:247.
[23]Calderone A,Dechamplain J,Rouleau JL.Adriamycin induced changes tothe myocardial beta-adrenergic system in the rabbit [J].J Med Cell Cardiol,1994,23:333.
[24]薛茂强,高音,李霄凌,高喜仁.温针对异丙肾上腺素所致的大鼠急性心肌损伤保护的组化观察[J].齐齐哈尔医学院学报,1998, 19(1): 1-3.
[25]谷万里,史载祥.中药保护心肌缺血损伤的非循环机制研究进展[J].中西医结合心脑血管病杂志,2005, 3(5): 422-424.
[26]姜桂美,于华荣,王艳丽.针灸防治恶性肿瘤放化疗所致骨髓抑制的研究进展[J].江苏中医,2000,21(6):45-46.
[27]崔瑾,申定珠,熊芳丽.针刺、艾灸膈俞穴对低白细胞模型大鼠白细胞及骨髓造血功能的调节作用[J].上海针灸杂志,2005,6,24(6):41-43.
[28]郁美娟,杨金洪,陈桂萍,等.针刺治疗放化疗所致造血功能损害的临床观察[J].中国针灸, 1994, 14(5): 4-6.
[29]李晔,于耀才,戴铁成.针刺治疗恶性肿瘤放化疗副反应的临床研究[J].中国针灸,1997,(6):327-328.
[1]Macrides TA,Naylor LM,Kalafatis N,etal. Hepatoprotective effects of the shark bile salt 5 betascymnolon acetaminoph-induced liver damage in mice[J]. Fundam Appl Toxicol,1996,33:31-37.
[2]Sarieh TC , Zhou T , Adams SP . A model of isoniazid induced hepatotoxicity in rabbits[J]. Pharmacol Toxicol Methods,1995,34(2):109-116.
[3]创斌,淤泽溥,林青,等.去脂软肝丸对四环素致小鼠脂肪肝模型肝脂的影响[J].云南中医学院学报,2002,25(1):8-10.
[4]林京玉,丁日高.抗菌药和药物性肝损伤[J].国外医学·药学分册,2006 33(3):195-197.
[5]孙成春,郝俊文,尹秋霞,等.汉防己甲素对环孢素A致小鼠肝肾损伤的作用[J].中国中药杂志,1998,23(6):373-374.
[6]李钦民,韩真.茶多酚对雷公藤内酯醇致小鼠肝损害的保护用[J].世界华人消化杂志,2006,14(9):908-911.
[7]唐云安,刘玉清,王国钦.肝损伤动物模型研究进展[J].卫生毒理学杂志,2002,16﹝4﹞:236-238.
[8]Berger ML,Bhatt H ,Combes B ,et al. CC14-induced toxicity in isolated hepatocytes[J]. Hepatology,1986,6:36-45.
[9]Decker K,Keppler D.Galactosamine induced liver injury[J]. Prog Liver Dis,1972,4:183-199.
[10]Bruck R,Shirin H ,Aeed H et al.Prevention of hepaticcirrhosis in rats by hydroxyl radical scavengers[J]. Hepatol,2001,35(3):457-464.
[11]王宪波,刘平,陆雄,等.二甲基亚硝胺大鼠肝纤维化形成中门脉压力的动态变化[J].世界华人消化杂志,2002,10(4) :401-405
[12] Ishigami M,Nishimura H ,Naiki Y ,et a1.The roles of intrahepatic valphal4(+)NK1.1(+)T cells for liver injury induced by salmonella infection in mice[J].Hepatology,1999,29(6):1799-1808.
[13]Jarvelainen HA,Fang C,Ingelman SM ,et a1.Effect of chronic coadministration of endotoxin and , ethanol on rat liver pathology and proinflammatory and antiinflammato-ry eytokines[J].Hepatology,1999,29(5):1503-1510.
[14]赵健雄,吴红梅,白德成,等.大鼠镍染毒致肝细胞部分离子变化及中药的治疗作用[J].中国职业医学,2004,31(6):31-33.
[15]孙应彪,朱玉真.镍铬钴混合物对小鼠肝脏毒作用机制的研究[J].中国公共卫生,2003,19(2):161-162.
[16]刘起展,董国宾,崔瑞平,等.刺梨汁饮料对锰中毒脂质过氧化损害的拮抗作用[J].卫生毒理学杂志,1999,13(1):42-44.
[17]于淑兰,邓一夫,王悦,等.锰、铅联合染毒对小鼠肝内部分生化指标的影响[J].卫生毒理学杂志,2000,14(4):228-229.
[18]陈永康,针灸足三里HbaAg携带者免疫功能影响的研究[J].中国针灸,1993,13(2):33-35.
[19]姚俊青,诚杰,笃一,针灸对大白鼠实验性肝损伤影响[J].上海针灸杂志,1995,14(5):234.
[20]周冬松,张利.电针内关、足三里为主治疗呃逆42例[J].中国针灸,2002,22(7):470.
[21]杨金洪.针刺防治环磷酰胺所致大鼠肝肾功能及脏器损害的作用[J].中国针灸,l99B.13(4):32-33.
[22]朱兆洪,丁柱,汤希盂,等.针刺“足三里”对脾虚小鼠脑组织中NO和NOS影响的实验研究[J].中国针灸,2000,20(5):309-311.
[23]马振亚.针刺对正常动物IL-2.IFN.NKC免疫调节网的影响[J].西安医科大学学报,1991,12(2):ll6-1l9.
[24]孙世晓,曾艳,张韵娴.针刺“曲泉”穴对兔胆汁分泌影响观察[J].针灸临床杂志,2001,l7(1):49.
[25]张茹华,周建华,王翰如,等.电针阳陵泉穴对兔胆囊压力变化的影响[J].山东中医学院学报,l995,19(3):197-198.