铜胺氧化酶催化产生的过氧化氢参与乙烯诱导蚕豆气孔关闭
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摘要
气孔是植物叶片与外界进行气体交换的主要通道,气孔运动受各种环境因素和内源因子包括植物激素乙烯和重要信号分子过氧化氢(H2O2)的调节。研究发现铜胺氧化酶(CuAO)催化产生的H2O2参与脱落酸(ABA)诱导的气孔关闭,乙烯诱导气孔关闭依赖于NADPH(还原型烟酰胺腺嘌呤二核苷酸磷酸)氧化酶催化产生的H2O2。然而时至今日,CuAO催化产生的H2O2是否参与乙烯诱导的气孔关闭还未见报道。本文以蚕豆为材料,借助药理学实验、激光扫描共聚焦显微镜技术和CuAO酶活性测定研究了CuAO及其催化产物H2O2在乙烯诱导气孔关闭中的作用。所得主要结果如下：
1.乙烯释放剂乙烯利、乙烯合成前体1-氨基环丙烷-1-羧酸(ACC)和乙烯均显著诱导气孔关闭。
2.CuAO专一性抑制剂氨基胍(Aminoguanidine. AG)、2-溴乙胺(2-Bromoethylamine. BEA)均显著抑制乙烯利、ACC和乙烯诱导气孔关闭。
3.CuAO的四种催化产物中仅H2O2能够显著逆转AG、BEA阻止乙烯利、ACC、乙烯诱导气孔关闭的效应。
4. CuAO催化底物腐胺(putrescine. Put)不影响乙烯利、ACC和乙烯诱导气孔关闭。
5.乙烯利、ACC和乙烯显著提高质外体CuAO活性,AG、BEA显著抑制乙烯利、ACC和乙烯提高质外体CuAO活性的效应。
6.乙烯利、ACC和乙烯显著增加保卫细胞H2O2水平,AG、BEA显著抑制乙烯利、ACC和乙烯增加保卫细胞H2O2水平的效应。
上述结果表明,乙烯通过提高质外体CuAO活性和H2O2水平诱导气孔关闭。
Stomatal is the main channel in plant leaves for gas exchange with the outside world, stomatal movement is regulated by multiple environment factors and internal cues including phytohormone ethylene and important signal molecule hydrogen peroxide (H2O2). Previous study has indicated that copper amine oxidase (CuAO) in Vicia faba guard cells is an essential enzymatic source for H2O2 production in ABA-induced stomatal closure via the degradation of putrescine, and ethylene-induced stomatal closure has also been shown to be dependent on H2O2 generated by the nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase. However, up to data, it is unclear whether H2O2 generated by CuAO participates in ethylene-induced stomatal closure. In the present study, using the abaxial epidermis of Vicia faba as materials, by means of the epidermal strip bioassay. laser scanning confocal microscopy (LSCM) and determination of CuAO activity, the role of H2O2 generated by CuAO in ethylene-induced stomatal closure was investigated. The main conclusions are as follows:
1. Ethylene release agent ethephon. ethylene precursor 1-aminocyclopropane-l-carboxylic acid (ACC) and ethylene induced stomatal closure significantly.
2. Aminoguanidine (Aminoguanidine. AG) and 2-bromine ethylamine (2-Bromoethylamine. BEA), two specific inhibitor of CuAO. obviously inhibited ethephon-, ACC-and ethylene-induced stomatal closure.
3. Among four catalytic products of CuAO. only H2O2 significantly reversed the inhibitory effects of AG and BEA on ethephon-, ACC-and ethylene-induced stomatal closure.
4. Putrescine (Put), a substrate of CuAO, had no significant effect on ethephon-, ACC-and ethylene-induced stomatal aperture.
5. Ethephon, ACC and ethylene induced increase in apoplastic CuAO activity, and the effects of ethephon, ACC and ethylene on CuAO activity were significantly prevented.
6. Ethephon. ACC and ethylene induced H2O2 production in guard cells, and the effects of ethephon. ACC and ethylene on H2O2 production in guard cells were significantly suppressed.
In summary, our results demonstrate that ethylene-induce stomatal closure is associated with CuAO-dependent H2O2 production.
1.乙烯释放剂乙烯利、乙烯合成前体1-氨基环丙烷-1-羧酸(ACC)和乙烯均显著诱导气孔关闭。
2.CuAO专一性抑制剂氨基胍(Aminoguanidine. AG)、2-溴乙胺(2-Bromoethylamine. BEA)均显著抑制乙烯利、ACC和乙烯诱导气孔关闭。
3.CuAO的四种催化产物中仅H2O2能够显著逆转AG、BEA阻止乙烯利、ACC、乙烯诱导气孔关闭的效应。
4. CuAO催化底物腐胺(putrescine. Put)不影响乙烯利、ACC和乙烯诱导气孔关闭。
5.乙烯利、ACC和乙烯显著提高质外体CuAO活性,AG、BEA显著抑制乙烯利、ACC和乙烯提高质外体CuAO活性的效应。
6.乙烯利、ACC和乙烯显著增加保卫细胞H2O2水平,AG、BEA显著抑制乙烯利、ACC和乙烯增加保卫细胞H2O2水平的效应。
上述结果表明,乙烯通过提高质外体CuAO活性和H2O2水平诱导气孔关闭。
Stomatal is the main channel in plant leaves for gas exchange with the outside world, stomatal movement is regulated by multiple environment factors and internal cues including phytohormone ethylene and important signal molecule hydrogen peroxide (H2O2). Previous study has indicated that copper amine oxidase (CuAO) in Vicia faba guard cells is an essential enzymatic source for H2O2 production in ABA-induced stomatal closure via the degradation of putrescine, and ethylene-induced stomatal closure has also been shown to be dependent on H2O2 generated by the nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase. However, up to data, it is unclear whether H2O2 generated by CuAO participates in ethylene-induced stomatal closure. In the present study, using the abaxial epidermis of Vicia faba as materials, by means of the epidermal strip bioassay. laser scanning confocal microscopy (LSCM) and determination of CuAO activity, the role of H2O2 generated by CuAO in ethylene-induced stomatal closure was investigated. The main conclusions are as follows:
1. Ethylene release agent ethephon. ethylene precursor 1-aminocyclopropane-l-carboxylic acid (ACC) and ethylene induced stomatal closure significantly.
2. Aminoguanidine (Aminoguanidine. AG) and 2-bromine ethylamine (2-Bromoethylamine. BEA), two specific inhibitor of CuAO. obviously inhibited ethephon-, ACC-and ethylene-induced stomatal closure.
3. Among four catalytic products of CuAO. only H2O2 significantly reversed the inhibitory effects of AG and BEA on ethephon-, ACC-and ethylene-induced stomatal closure.
4. Putrescine (Put), a substrate of CuAO, had no significant effect on ethephon-, ACC-and ethylene-induced stomatal aperture.
5. Ethephon, ACC and ethylene induced increase in apoplastic CuAO activity, and the effects of ethephon, ACC and ethylene on CuAO activity were significantly prevented.
6. Ethephon. ACC and ethylene induced H2O2 production in guard cells, and the effects of ethephon. ACC and ethylene on H2O2 production in guard cells were significantly suppressed.
In summary, our results demonstrate that ethylene-induce stomatal closure is associated with CuAO-dependent H2O2 production.
引文
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