不同锰源和锰水平对肉仔鸡组织锰含量及心肌细胞凋亡的影响
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摘要
本研究初步探讨了玉米-豆粕型基础饲粮中添加不同形态锰源及锰水平对肉仔鸡生长性能、组织锰含量、心肌细胞中自由基(MDA和NO)含量、自由基代谢相关酶(MnSOD和NOS)的活性及心肌细胞凋亡的影响。试验选取320只商品代1日龄AA肉仔鸡,其饲粮中分别添加中等络合强度复合氨基酸锰和无机硫酸锰2种锰源,每种锰源设0 mg/kg、100 mg/kg、200mg/kg3个锰添加水平,按照2×3两因子进行试验设计,两种锰源共用一个不加锰的对照组,共组成5个处理组。试验期42天。研究结果表明:
1.锰源和锰水平及两者的互作对1～21日和22～42日龄肉仔鸡的ADG和ADFI及1～22日龄F/G均无显著影响(P>0.10),但22～42日龄肉仔鸡添加100mg/kg锰组的F/G比值显著低于对照组和200mg/kg锰组(P<0.10)。
2.21日龄肉仔鸡跖骨灰、胰脏和心肌锰含量及42日龄肉仔鸡脾脏、肝脏、胰脏和心肌锰含量均随饲粮添加锰水平升高而明显升高(P <0.10),但不同锰源间无显著差异(P>0.10)。锰源与锰水平互作显著影响21日龄肉仔鸡肾脏和脾脏锰含量(P <0.10),当添加锰水平为200 mg/kg时,添加有机锰组明显高于添加硫酸锰组(P <0.10)。42日龄肉仔鸡添加锰组肾脏和跖骨灰锰含量均显著高于对照组(P<0.10),添加中等络合强度复合氨基酸锰组肾脏和跖骨灰锰含量明显高于添加无机硫酸锰组(P <0.10)。
3. 21和42日龄肉仔鸡心肌细胞线粒体中MnSOD mRNA水平、MnSOD蛋白表达水平和MnSOD活性试验组均显著高于对照组(P<0.10),但锰源及锰源与锰水平互作对其无显著影响(P>0.10)。
4.21日龄肉仔鸡心肌细胞NOS活性未受锰源、锰水平及其互作的影响(p>0.10 ),但锰水平显著影响42日龄肉仔鸡心肌细胞NOS活性(P<0.10),即加锰组NOS活性明显低于对照组。
5.锰源及锰源与锰水平互作对21和42日龄肉仔鸡心肌细胞中MDA和NO含量均无显著影响(P>0.10),但饲粮添加锰组其心肌细胞MDA和NO含量均低于对照组(P<0.10)。
6.21和42日龄肉仔鸡心肌细胞凋亡率均随饲粮锰添加量的增加而明显降低(P<0.10),但锰源及锰源和锰水平互作对其均无显著影响(P>0.10)。
The thesis studied the effects of Mn sources and levels on growth performance, tissue Mn content, free radicals (MDA and NO) content,MnSOD and NOS activity of heart-cells and heart-cells apoptosis in broilers based incorn-soybean foundational ration. A total of 320 one-day-old broilers , added Mn-AAB and MnSO4·H2O two Mn sources to feed, each source located 0 mg/kg,100 mg/kg and 200 mg/kg 3 Mn levels, according to 2×3 factorial arrangement. 2 Mn sources shared one control group fed corn-soybean meal basal diet.The experiment was composed of five groups. randomly allotted to one of five groups for 42 days. The results showed:
1.The Mn source and Mn level or their interaction did not affect on ADG, ADFI and F/G of 1～21 days and ADG, ADFI of 22～42 days in broilers(P>0.10). But, the groups of fed diet added with 100mg/kg Mn had lower than the control group and 200mg/kg groups in22～42 days broilers(P <0.10).
2.The Mn concent of metatarsal ash, pancreatic, and heart in 21 days briolers and the Mn concent of spleen, liver, pancreatic and heart in 42 days briolers were increased with the dietary Mn level increased(P <0.10), but there were no differences between the two Mn sources(P>0.10). The interaction between Mn sources and Mn levels significantly affected (P <0.10) the Mn concent of kidney and spleen in 21 days briolers, in which the Mn concent of spleen and kidney were higher (P<0.10) when supplemented with 200 mg/kg Mn from Mn-AAB than those from MnSO4·H2O. The Mn concent of metatarsal ash and kidney were higher when briolers fed diet supplemented Mn from Mn-AAB than MnSO4·H2O in 42 days briolers.
3.Heart MnSOD mRNA levels, MnSOD protein and MnSOD activity were influenced by dietary Mn levels (P <0.10) in 21 and 42 days broilers, but they no significantly affected by Mn sources (p <0.10).
4. Heart NOS activity of 21 days briolers were not influenced(P>0.10) by Mn sources,Mn levels and their interaction. But Mn levels significantly affected heart NOS activity (p <0.10) of 42 days broilers.
5.The Mn sources and the interaction between Mn sources and levels had no significant effects on heart MDA and NOcontents in 21 and 42 days briolers (P>0.10). whereas that of contents in experimental groups were lower than those in the control group (P <0.10).
6.The heart-cells apoptosis rates was significantly decreased as the dietary Mn levels increased in 21 and 42 days briolers (P<0.10), but, No effects of Mn sources and the interaction between Mn sources and Mn levels (p>0.37).
1.锰源和锰水平及两者的互作对1～21日和22～42日龄肉仔鸡的ADG和ADFI及1～22日龄F/G均无显著影响(P>0.10),但22～42日龄肉仔鸡添加100mg/kg锰组的F/G比值显著低于对照组和200mg/kg锰组(P<0.10)。
2.21日龄肉仔鸡跖骨灰、胰脏和心肌锰含量及42日龄肉仔鸡脾脏、肝脏、胰脏和心肌锰含量均随饲粮添加锰水平升高而明显升高(P <0.10),但不同锰源间无显著差异(P>0.10)。锰源与锰水平互作显著影响21日龄肉仔鸡肾脏和脾脏锰含量(P <0.10),当添加锰水平为200 mg/kg时,添加有机锰组明显高于添加硫酸锰组(P <0.10)。42日龄肉仔鸡添加锰组肾脏和跖骨灰锰含量均显著高于对照组(P<0.10),添加中等络合强度复合氨基酸锰组肾脏和跖骨灰锰含量明显高于添加无机硫酸锰组(P <0.10)。
3. 21和42日龄肉仔鸡心肌细胞线粒体中MnSOD mRNA水平、MnSOD蛋白表达水平和MnSOD活性试验组均显著高于对照组(P<0.10),但锰源及锰源与锰水平互作对其无显著影响(P>0.10)。
4.21日龄肉仔鸡心肌细胞NOS活性未受锰源、锰水平及其互作的影响(p>0.10 ),但锰水平显著影响42日龄肉仔鸡心肌细胞NOS活性(P<0.10),即加锰组NOS活性明显低于对照组。
5.锰源及锰源与锰水平互作对21和42日龄肉仔鸡心肌细胞中MDA和NO含量均无显著影响(P>0.10),但饲粮添加锰组其心肌细胞MDA和NO含量均低于对照组(P<0.10)。
6.21和42日龄肉仔鸡心肌细胞凋亡率均随饲粮锰添加量的增加而明显降低(P<0.10),但锰源及锰源和锰水平互作对其均无显著影响(P>0.10)。
The thesis studied the effects of Mn sources and levels on growth performance, tissue Mn content, free radicals (MDA and NO) content,MnSOD and NOS activity of heart-cells and heart-cells apoptosis in broilers based incorn-soybean foundational ration. A total of 320 one-day-old broilers , added Mn-AAB and MnSO4·H2O two Mn sources to feed, each source located 0 mg/kg,100 mg/kg and 200 mg/kg 3 Mn levels, according to 2×3 factorial arrangement. 2 Mn sources shared one control group fed corn-soybean meal basal diet.The experiment was composed of five groups. randomly allotted to one of five groups for 42 days. The results showed:
1.The Mn source and Mn level or their interaction did not affect on ADG, ADFI and F/G of 1～21 days and ADG, ADFI of 22～42 days in broilers(P>0.10). But, the groups of fed diet added with 100mg/kg Mn had lower than the control group and 200mg/kg groups in22～42 days broilers(P <0.10).
2.The Mn concent of metatarsal ash, pancreatic, and heart in 21 days briolers and the Mn concent of spleen, liver, pancreatic and heart in 42 days briolers were increased with the dietary Mn level increased(P <0.10), but there were no differences between the two Mn sources(P>0.10). The interaction between Mn sources and Mn levels significantly affected (P <0.10) the Mn concent of kidney and spleen in 21 days briolers, in which the Mn concent of spleen and kidney were higher (P<0.10) when supplemented with 200 mg/kg Mn from Mn-AAB than those from MnSO4·H2O. The Mn concent of metatarsal ash and kidney were higher when briolers fed diet supplemented Mn from Mn-AAB than MnSO4·H2O in 42 days briolers.
3.Heart MnSOD mRNA levels, MnSOD protein and MnSOD activity were influenced by dietary Mn levels (P <0.10) in 21 and 42 days broilers, but they no significantly affected by Mn sources (p <0.10).
4. Heart NOS activity of 21 days briolers were not influenced(P>0.10) by Mn sources,Mn levels and their interaction. But Mn levels significantly affected heart NOS activity (p <0.10) of 42 days broilers.
5.The Mn sources and the interaction between Mn sources and levels had no significant effects on heart MDA and NOcontents in 21 and 42 days briolers (P>0.10). whereas that of contents in experimental groups were lower than those in the control group (P <0.10).
6.The heart-cells apoptosis rates was significantly decreased as the dietary Mn levels increased in 21 and 42 days briolers (P<0.10), but, No effects of Mn sources and the interaction between Mn sources and Mn levels (p>0.37).
引文
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86臧莹安,丁发源,王小龙,等.PHS对快大型黄羽肉仔鸡线粒体NO代谢和Na+一K+一ATP酶活力的影响[J].中国兽医学报,2008,28(3):306—309.
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