2型糖尿病周围神经病变与血清TAOC、MDA、SOD的相关性研究
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摘要
目的:探讨2型糖尿病患者(T2DM)血清总抗氧化能力(TAOC)、血清丙二醛(MDA)、血清超氧化物歧化酶(SOD)水平与糖尿病周围神经病变(DPN)的相关性。方法:测定对照组52例及122例2型糖尿病患者血清总抗氧化能力(TAOC)及丙二醛(MDA),超氧化物歧化酶(SOD)水平;同时记录2型糖尿病患者的血压、身高、体重、血肌酐(Scr)、血尿酸(UA)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、甘油三酯(TG)、总胆固醇(TC)、纤维蛋白原(FIB)、空腹血糖(FPG)、糖化血红蛋白(HbA1c)、计算体重指数(BMI)。采用肌电图测定神经传导速度(NCV),神经电生理正常者为糖尿病非周围神经病变组(非DPN组)33例,神经电生理异常者为糖尿病周围神经病变组(DPN组)89例。采用SPSS11.5统计软件进行数据处理,所有计量资料用(均数±标准差)表示,进行t检验;计数资料进行χ2检验。以有无DPN为应变量进行多因素Logistic回归分析。两变量之间采用spearman秩相关分析。结果:对照组较2型糖尿病患者TAOC、SOD水平高,MDA水平低,有统计学差异(P<0.05),非DPN组与DPN组比较神经传导速度有统计学差异(P<0.05),非DPN组与DPN组比较体重指数(BMI)、收缩压(SBP)、舒张压(DBP)、空腹血糖(FPG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、血肌酐(Scr)、血尿酸(UA),糖化血红蛋白(HbAIc )、超氧化物歧化酶(SOD)无统计学差异(P>0.05),而年龄、病程、FIB、TAOC、MDA在两组间有统计学差异(P<0.05),通过多因素Logistic回归分析:MDA、年龄、病程进入回归方程(P<0.05)。单因素分析中MDA与TAOC、SOD呈负相关,与病程、年龄及FIB呈正相关。结论:本组资料显示,随着年龄的增长、病程的延长、患者的MDA水平的增高与糖尿病周围神经病变的发生可能存在相关性。MDA可能参与了糖尿病周围神经病变的发生,氧化与抗氧化之间的失衡,可能是造成糖尿病周围神经病变发生的原因之一。此外,还应注意血液的高凝状态。
Objective:To investigate of the correlation of Serum TAOC、MDA、SOD with T2DMdiabetic peripheral neuropathy。Method:serum level of the total antioxidant capacity (TAOC)and Malondialdehyde(MDA)and superoxide dismutase(SOD)were measured in 122 type 2 diabetes and in control group 52 subjects。meanwhile,Their blood pressure,body height, body weight,,serum creatinine(Scr), blood uric acid(UA), highdensity lipoprotein(HDL) ,lowdensity lipoprotein(LDL) ,triglyceride(TG),total cholestero(lTC), serum fibrinogen(FIB), Fasting blood glucos(eFPG)、glycosylated hemoglobin(HbAIc) were recorded; body mass index(BMI) were calculated。To use electromyogram to measure nerve conduction velocity( NCV )neuroelectricity electrophysiology normal is non- diabetic peripheral neuropathy(non-DPN)34 subjects;neuroelectricity electrophysiology abnormal is diabetic peripheral neuropathy(DPN)89 subjects。Statistics software SPSS11.5 was used for data processing. All the measurement data was expressed as (mean±standard deviation) and was analyzed by t-test; the numerical data was analyzed by Chi-square test. Logistic regression. was analyzed by means of with or without nodiabetic peripheral neuropathy as dependent variabelity.between two variables use to spearman rank correlation analysis. Results:control group serum level of the total antioxidant capacity (TAOC)and serum level of superoxide dismutase(SOD)is higher than diabetes group,serum level of Malondialdehyde(MDA)is lower than diabetes group。It has statistical significance between two groups(P<0.05)。nerve conduction velocity had statistical significance between two groups(P<0.05)。Body mass index,systolic blood pressure(SBP),diastolic blood pressure(DBP)、Fasting blood glucose、triglyceride、total cholesterol、highdensity lipoprotein、lowdensity lipoprotein、serum creatinine、blood uric acid、glycosylated hemoglobin、superoxide dismutase had no statistical significance between two groups(P>0.05)。as well as,age、duration of diabetes、TAOC、MDA、FIB had statistical significance between two groups(P<0.05),Analysis of Logistic regression showed that age、duration of diabetes and MDA level were the positive correlation factor of diabetic peripheral neuropathy(P<0.05)。MDA leval was correlated negative with TAOC、SOD;duration of diabetes、age、FIB was positive correlated with MDA。Conclusions:MDA level, age and duration of diabetescorrelated positively with diabetic peripheral neuropathy; MDA may participate in the development of type 2 diabetic peripheral neuropathy.Banlance disorder between with Oxidation and antioxygen may be one of the reasons diabetic peripheral neuropathy。Otherwise,we should be pay attention to hematic hypercoagulabale state.
Objective:To investigate of the correlation of Serum TAOC、MDA、SOD with T2DMdiabetic peripheral neuropathy。Method:serum level of the total antioxidant capacity (TAOC)and Malondialdehyde(MDA)and superoxide dismutase(SOD)were measured in 122 type 2 diabetes and in control group 52 subjects。meanwhile,Their blood pressure,body height, body weight,,serum creatinine(Scr), blood uric acid(UA), highdensity lipoprotein(HDL) ,lowdensity lipoprotein(LDL) ,triglyceride(TG),total cholestero(lTC), serum fibrinogen(FIB), Fasting blood glucos(eFPG)、glycosylated hemoglobin(HbAIc) were recorded; body mass index(BMI) were calculated。To use electromyogram to measure nerve conduction velocity( NCV )neuroelectricity electrophysiology normal is non- diabetic peripheral neuropathy(non-DPN)34 subjects;neuroelectricity electrophysiology abnormal is diabetic peripheral neuropathy(DPN)89 subjects。Statistics software SPSS11.5 was used for data processing. All the measurement data was expressed as (mean±standard deviation) and was analyzed by t-test; the numerical data was analyzed by Chi-square test. Logistic regression. was analyzed by means of with or without nodiabetic peripheral neuropathy as dependent variabelity.between two variables use to spearman rank correlation analysis. Results:control group serum level of the total antioxidant capacity (TAOC)and serum level of superoxide dismutase(SOD)is higher than diabetes group,serum level of Malondialdehyde(MDA)is lower than diabetes group。It has statistical significance between two groups(P<0.05)。nerve conduction velocity had statistical significance between two groups(P<0.05)。Body mass index,systolic blood pressure(SBP),diastolic blood pressure(DBP)、Fasting blood glucose、triglyceride、total cholesterol、highdensity lipoprotein、lowdensity lipoprotein、serum creatinine、blood uric acid、glycosylated hemoglobin、superoxide dismutase had no statistical significance between two groups(P>0.05)。as well as,age、duration of diabetes、TAOC、MDA、FIB had statistical significance between two groups(P<0.05),Analysis of Logistic regression showed that age、duration of diabetes and MDA level were the positive correlation factor of diabetic peripheral neuropathy(P<0.05)。MDA leval was correlated negative with TAOC、SOD;duration of diabetes、age、FIB was positive correlated with MDA。Conclusions:MDA level, age and duration of diabetescorrelated positively with diabetic peripheral neuropathy; MDA may participate in the development of type 2 diabetic peripheral neuropathy.Banlance disorder between with Oxidation and antioxygen may be one of the reasons diabetic peripheral neuropathy。Otherwise,we should be pay attention to hematic hypercoagulabale state.
引文
[1] Zimmet P. , Alberti K. G. , Shaw J. , et al. Global and societal implications of the diabetes epidemic. Nature, 2001, 414:782-787.
[2] Vinik AI, Vinik E. Prevention of the complications of dia betes. Am J Manag Care 2003;9(3Suppl):S63-80;quizS 81-84
[3]张春芳,钟历勇,张在强. 2型糖尿病严重周围神经病变1例[J].中国疼痛医学杂志, 2006, 12(4):201~202
[4] BRIL V, WERB MR, GREENE DA, et al, Single fiber Electromyo grapy in Diaetic Peripheral Polyneuropthy[J]. MusecleNerve(S0148-639X), 1996, 19(1):2-9
[5] Brownlee M. The pathobiology of diabetic complications:a unifying mechanism. Diabetes 2005;54:1615-1625.
[6] Feldman EL. Oxidative stress and diabetic neuropathy:a new understanding of an old problem[J]. JClinInvest, 2003, 111(4):431 -433.
[7] van DAMPS. Oxidative stress and diabetic neuropathy: pathophys-Iological mechanisms and treatment perspectives[J]. Diabetes Metab ResRev, 2002, 18(3):176-184.
[8] Nair N, Bedwal S, Prasad S, et al. Short term zinc deficiency in diet induces increased oxidative stress in testes and epididymis of rats[J]. Indian J exp Biol, 2005, 43(9):786 -794.
[9] Lal MA, KomerA, MatsuoY, etal. Combined antioxidant and COMT inhibitor treatment reverses renal abnorinalities in diabetic rats[J]. Diabetes,Aug 2000;49:1381-1389
[10]Kasuga M. Insulin resistance and pancreatic B cell failure. J CliInvest, 2006; 116:1756-1760
[11]裴凌鹏,金宗濂.葛根黄酮改善小鼠抗氧化功能的研究[J].营养学报, 2005, 26(6):505-506
[12]张文清,海春旭,龚书明.锌对大鼠肝微粒体脂质过氧化模型抗氧化作用的实验研究[J].解放军预防医学杂志, 1998, 16(4):250- 254.
[13]Grawel S, Wardas M, Niedworok E, et al. Malondialdehyde(MDA)as a lipid peroxidation marker[J]. Wiad Lek, 2004, 57(9-10):453- 455.
[14]Ceriello A, Bortolotti N, Falleti E, Taboga C, Tonutti L, Crescentini A, Motz E, Lizzio S, Russo A, Bartoli E 1997 Total radical-trapping antioxidant parameter in NIDDM patients. Diabetes Care 20:194–197
[15]Han XY, Xu ZR, Wang YZ, et al?Effect of cadm ium on Lipid peroxdation and activites of antioxdant enzymes in growing pigs?biolTiace Elem Res 2006;110 30):251-263
[16]Blaszczak R, Kujawski K, Kedziora Kornatowska K, etal The total antioxidant capacity and low-molecular antioxidant concentration in plasma of type 2 diabetes patients with different stage of metabolic compensation and concomitant diabetic nephropathy[J]. PolMerkuriuszLek, 2005, 18(103):29 -32.
[17]Bailey MA, Sanat KS A novel anti-oxidant and anti-cancer strategy:a peptoid anti-inflammatory drug conjugate with SOD mimic activity〔J〕. Biochem Biophys Res Commun, 2004;317(4):1155- 8.
[18]Hartnett ME , Stratton RD, Browne RW, et al. Serum markers of ox idative stress and severitv of diabetic retinopathy. Diabetes Care2000, 23(2):234-40.
[19]栾松,崔丽英,汤晓芙,等.糖尿病周围神经病运动神经传导速度分布.中华神经科杂志, 2006, 39(7):436 -439.
[20]吕文山,董砚虎,钱荣立.糖尿病的诊断与分型[J].中国糖尿病杂志, 2000, 8(1): 60-61
[21]苏雅茹,杨莉芹,张云云,等.血糖控制与糖尿病多发性神经病变患者临床表现的观察[J].中国糖尿病杂志, 2003, 11(6):442 -443.
[22].奉水东,凌宏艳?高果糖饲料诱导SD大鼠氧化应激及对其脂质代谢的影响?中华慢性病预防与控制?2005?13(1):7-8
[23]刘志民,孙亮亮氧化应激在糖尿病发病机制中作用的认识?内科理论与实践2007年第2卷第3期?153-155
[24]陈瑗,周玫.自由基医学基础与病理生理[M].北京:人民卫生出版社, 2002. 24-25.
[25]Nagley P, Zhang C, Lim ML, et al. Mitochondrial DNA deletions parallel age-linked decline in rat sensory nerve function[J]. Neurobiol Aging, 2001, 22(4):635-43.
[26]Fridlyand LE, Philipson LH. Does the glucose dependent insulin secretion mechanism itself cause oxidative stress in pancreaticβ3/Ccells?[J]. Diabetes, 2004, 53(8):1942 -1948.
[27]Kadenbach B. Intrinsic and extrinsic uncoupling of oxidative phosphory-lation[J]. Biochim. Biophys Acta, 2003, 1604(2):77-94.
[28]Guzik TJ, Mussa S, Gastaldi D, et al. Mechanisms of increased vascular superoxide production in human diabetes mellitus:role of NAD(P)H oxidase and endothelial nitric oxide synthase[J]. Circulation, 2002, 105(14):1656-1662.
[29]Ergul A, Schultz Johansen J, Stromhaug C, et al. Vascular dysfunction of venous bypass conduits is mediated by reactive oxygen species in diabetes:Role of endothelin-1 [J]. J Pharmacol Exp Ther, 2004, 313:70-77.
[30]Green K, Brand MD, Murphy MP. Prevention of mitochondrial oxidative damage as a therapeutic strategy in diabetes[J]. Diabetes, 2004, 53(Suppl1):S110-118.
[31]MARITIM A C, SANDERS R A, WATKINS J B 3rd. Diabetes, oxidative stress, and antioxidants:a review[J]. J Biochem Mol Toxicol, 2003, 17(1):24-38.
[32]王忠山,邹冬辉,赵慧,等.短期糖尿病雄性大鼠前列腺自由基和抗氧化水平变化研究[J].中国病理生理杂志2003, 19(6):803-806.
[33]屈智慧,王旭渤,李永勇,等.糖尿病大鼠肾脏氧化应激的实验研究[J].中国实验诊断学, 2004, 8(2):193-195.
[34]Du Y, Miller CM, Kern TS. [J]. Free Radic BiolMed, 2003, 35(11):1491-1499
[35]Haskins K, Bradley B, Powers K, et al. Oxidative stress in type 1 diabetes[J]. Ann NY Acad Sci, 2003, 1005(11)43-54.
[36]Kowluru RA, Abbas SN, Odenbach S. [J]. J Diabetes Complications, 2004, 18(5):282-288
[37]Stentz FB, Umpierrez GE, Cuervo R, et al. Proinflamma tory cytokines, markers of cardiovascular risks, oxidative stress, and lipid peroxidation in patients with hyperglyce miccrises[J]. Diabetes, 2004, 53(8):2079-2086.
[38]Marra G, Cotroneo P, Pitocco D. [J]. Diabetes Care, 2002, 25(2):370-375
[39]Ceriello A , Mercuri F, Quagliaro L, et al. Detection of nitrotyrosine in the diabetic plasma: evidence of oxidative stress. Diabetologia, 2001, 44:834-838
[40]Nishikawa T, Sasahara T, Kiritoshi S, et al. Evaluation of urinary8- hydroxydeoxy-guanosine as a novel biomarker of macrovascular complications in type 2 diabetes[J]. DiabetesCare, 2003, 26(5):1507-1512.
[41]Nishikawa T, Sasahara T, Kiritoshi S, et al. [J]. Diabetes Care, 2003, 26:1507-1512
[42]Wu LL , Chiou CC , Chang PY, et, al. Urinary 8-OHDG:a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetic. Clin Chim Acta, 2004, 339:1-9
[43]Shin CS, Moon BS, Park KS, et al. Serum 8-hydroxy-guanine levels are Increased in diabetic patients [J]. Diabetes Care, 2001, 24(4):733-737.
[44]Nobecourt E J acquerminet S, Hansel B, et al. Defective antioxidative activity of small dense HDL3 particles in type 2 diabetes: relationship to elevated oxidative stress and hyperglycaemia. Diabetologia, 2005, 48:529-538
[45]Stentz FB, Umpierrez GE, Cuervo R, et al. [J]. Diabetes, 2004, 53(8):2079-2086.
[46]Ziegler MD, Sohr CG, Nourooz-Zzdeh J. Oxidative stress and antioxidant defence in relation to the severity of diabetic polyneuropathy and cardiovascular autonomic neuropathy . Diabetes Care, 2004, 27:2178-2183.
[47]于翰柳洁糖尿病周围神经病变的研究进展山西医药杂志2007年9月第36卷第9期731-734
[48]Mohanty P, Hamouda W, Garg R, et al. Glucose challenge stimulates reactive oxygen species(ROS)generation by leucocytes. J Clin Endocrinol Metab 2000;85(8)∶2970-2973.
[49]Mentink CJ, Kilhovd BK, Rondas- Colbers GJ, etal. Time course of specific AGEs during optimized glycaemic control in type 2 diabetes. Neth J Med 2006;64(1)∶10-16.
[50]Tsukahara H, Sekine K, Uchiyama M, et al. Formation of advanced glycosylation end products and oxidative stress in young patients with type 1 diabetes. Pediatr Res 2003;54 (3)∶419-424.
[51]Usui T, Shizuuchi S, Watanabe H, et al. Cytotoxicity and oxidative stress induced by the glyceraldehyde-relatedmaillard reaction products for HL-60cells. Biosci Biotechnol Biochem 2004;68(2)∶333-340.
[52]Wauter MP, Chappey O, Corda S, et al. Activation of NADPH oxidase by AGE links oxidant stress to altered gene expression via RAGE. Am Jphysiol Endocrinol Metab2001; 280(5)∶E685-E694.
[53]Cellek S, Qu W, Schmidt AM, et al. Synergistic action of advanced glycation end products and endogenous nitric oxide leads to neuronal apoptosis in vitro:a new insight into selective nitrergic neuropathy in diabetes. Diabetologia2004;47 (2)∶331-339
[54]Kubo E, Urakami T, Fatma N, et al. Polyol pathway dependent osmotic and oxidative stresses in aldose reductase-mediated apoptosis in human lens epithelial cells:role of AOP2, Biochem Biophys Res Commun2004;314(4)∶1050-1056.
[55]Oyama T, Miyasita Y, Watanabe H, et al. The role of polyolpathway in high glucose -induced endothelial cell damages. Diabe Res Clin Pract 2006;73(3)∶227-234.
[56]Takeshi Suzuki, Hiromi Sekido, Noriaki Kato, et al. Neurotrophin- 3 induced production of nerve growth factor is suppressed in Schwann cells exposed to high glucose:involvement of the polyolpathway. Neurochemistry2004;91(6)∶1430-1438.
[57]Song ZT, Fu DT, Chan YS, et al. Transgenic mice overex pressing aldose reductase inSchwann cells show more severe nerve conduction velocity deficit and oxidative stress under hyperglycemic stress. Mol Cell Neurosci 2003;23(4)∶638-647.
[58]Cosentino F, Eto M, De Paolis P, et al. High glucose causes Upregulation of cyclooxygenase -2 and alters prostanoid profile in human end othelialcells:role of protein kinase Candreactive oxygen species. Circulation2003;107(7)∶1017-1023.
[59]Toshiyo Sonta, Toyoshi Inoguchi, Hirotaka Tsubouchi, et al. Evalution of oxidative stress in diabetic animals by invivo electron spin resonance measurement-role of protein kinase C. Diab Res Clin Pract 2004;66(Suppl1)∶S109-S113.
[60]Pricci F, Leto G, Amadio L, et al. Oxidative stress in diabetes induced end othelial dysfunction in volvement of nitricoxide and protein kinase C. Free Radic Biol Med 2003;35(6)∶683-694.
[61]Koh DW, Dawson TM, Dawson VL, Mediation of cell death by poly(ADP ribose)polymerase -1. PharmacolRes2005;52(1)∶5-14.
[62]Obrosova IG, Mabley JG, Zsengeller Z, et al. Role for ni trosative stress in diabetic neuropathy:evidence from studies with a peroxynitrite decomposition catalyst. FASEB J 2005;19(3)∶401-403.
[63]Obrosova IG, Drel VR, Pacher P, et al. Oxidative -nitrosative stress and poly(ADP -Ribose)plymerase(PARP)activation in experiment al diabetic neuropathy:the relation is revisited. Diabetes2005;54(12)∶3435-3441.
[64]Fukunaga M, Miyata S, Liu BF, et al. Methylglyoxal induces apoptosis through activation of p38 MAPK in rat Schwanncells. Biochem Biophy Res Commun2004; 320(3)∶689-695.
[65]Vincent AM, McLean LL, Backus C, et al. Short-termhy perglyce mia produces oxidative damage and apoptosis in neurons. FASEBJ2005;19(6)∶638-640.
[66]朱禧星.现代糖尿病学[M].第1版.上海:上海医科大学出版社, 2000. 318 -319.
[67]L lewelyn JG. . The diabetic neuropathies: types, diagnosis and management[J]. Jneurol Neurosurg Psychiatry, 2003, 74∶5-7.
[68]Gill GV, Huddle KR, MonkoeG. Longterm(20years)outcome and mortality of type1 diabeteic patients in Soweto, SouthAfrica. Diabet Med, 2005, 22:1642 -1646.
[69]王桂侠,李广仁,刘立,等.糖尿病周围神经病变患者神经病变及临床研究[J].中风与神经疾病杂志, 2000, 17(5):277
[70]李素梅,叶山东,杨静,等. 2型糖尿病周围神经传导速度与病程及尿清蛋白的关系---附66例报告[J].新医学, 1999, 30(6):327 328.
[71]史轶蔡.协和内分泌和代谢学[M]. 2版北京:科学出版社2000: 1339 - 40.
[72]王薇彬.等2型糖尿病患者氧化应激状况及影响因素分析[J]中国糖尿病杂志2007年第15卷第8期, 487-489
[73]史玉泉.实用神经病学[M].第2版.上海:上海科技出版社, 1994. 986-989.
[1] Feldman EL. Oxidative stress and diabetic neuropathy :a new under-Standing of an old problem [J]. JClinInvest, 2003, 111(4):431-433.
[2]陈瑗,周玫.自由基医学基础与病理生理[M].第1版.北京:人民卫生出版社, 2002.
[3] Wu LL , Chiou CC, ChangPY, etal. Urinary8-OHdG:amarkerofoxida-Tive stress to DNA and a risk factor for cancer, atherosclerosis and dia-betics. ClinChimActa, 2004, 339:1-9.
[4] Sharma A, Kharb S , Chugh SN, et al. Evaluation of oxidative stress before and after control of glycemia and after vitaminE supplementation in diabetic patients. Metabolism, 2000, 49:160-162.
[5] Bonnefont RD , Bastard JP, Jaudon MC , et al . Consequences of the diabetic status on the oxidant/antioxidant balance . Diabetes Metab, 2000, 26:1632176.
[6] Du Y, Miller CM, Kern TS. [J]. Free Radic BiolMed, 2003, 35(11):1491-1499
[7] Hoeldtke RD , Bryner KD, McNeill DR, et al. [J]. Diabetes Care, 2002, 25:1439-1443。
[8] Kanauchi M, Nishioka H, Hashimoto T. [J] . Nephron, 2002, 91;327-329
[9] Nishikawa T, Sasahara T, Kiritoshi S, et al. [J]. Diabetes Care, 2003, 26:1507-1512
[10]Kowluru RA, Abbas SN, Odenbach S. [J]. J Diabetes Complications, 2004, 18(5):282-288
[11]Shin CS, Moon BS, Park KS, et al. Serum 8-hydroxy-guanine levels are Increased in diabetic patients [J]. Diabetes Care, 2001, 24(4):733-737.
[12]Nobecourt E J acquerminet S, Hansel B, et al. Defective antioxidative activity of small dense HDL3 particles in type 2 diabetes: relationship to elevated oxidative stress and hyperglycaemia. Diabetologia, 2005, 48:529-538
[13]Schmeichel AM, Schmelzer JD , Low PA. Oxidative injury and apoptosis of doesal root ganglion neurons in chronic experimental diabetic neuropathy , Diabetes, 2003, 52:165-171.
[14]Coppey LJ, Gellet JS, Davidson EP, et al . Effect of treating treptozotocin induced diabetic rats with sorbinil, myo-inositol oraminoguanidine on endoneurial blood flow, motor nerve conduction velocity and vascular function of epineurial arterioles of the sciatic nerve. Int J Exp Diabetes Res, 2002, 3:21-36.
[15]Wang Y, Schmeichel AM, Lida H, et al . Ischemia-reperfusion injury causes oxidativestress and apoptosis of Schwann cell in acute and chronic experimental diabetic neuropathy. Antioxid Redox Signal, 2005, 7;1513-1520.
[16]Feldman EL. Oxidative stress and diabetic neuropathy :a new understanding of an old problem . J Clin Invest, 2003, 111:434-433.
[17]Wautier MP, Chappey O, Corda S, et al. Activation of NAPDH oxidase by AGE Links oxidant stress to altered gene expression via RAGE. Am J Physiol , 2001, 280:685-694.
[18]Sekido h, Suzuki T, Jomori T, et al . Reduced cell replication and induction of apoptosis by advanced glycation end products in rat Schwarm cells, Biochem Biophys Res Commun, 2004, 320:241-248.
[19]Cellek S, Qu W , Schmidt AM , et al , Synergistic action of advanced glycation end products and endogenous nitric oxide leads to neuronal apoptpsis in votro: a new insight into selective nitrergic neuropathy in diabetes. Diabetologia, 2004. 47:331-339.
[20]Song Z, Fu DT, Chan YS, et al Transgenic mice over expressing aldose reductase in Schwann cells show more severe nerve conduction velocity deficit and oxidative stress under hyperglycemic stress, Mol Cell Neurosci, 2003, 23:638-647.
[21]Babior BM, NADPH oxidase Curr Opin Immunol . 2004. 16:42-47
[22]Oh-Hashi KMaruyama W, Isobe KP eroxynitrite induces GADD34, 45. and 153 VLA p38 MAPK in human neuroblastoma SH-SY5Y cells, Free Radic Biol Med, 2001, 30;213-221.
[23]Rosen P . Nawroth PP, King G, et al . The role of oxidative stress in the onset and progression of diabetes and its complications:a summary of a Congress Series sponsored by UNESCO-MCBN, the American Diabetes Association and the German Diabetes Society . Diabetes Metab Res Rev , 2001. 17:189-212.
[24]Aragno M. Mastrocola R, Bringnardello E , et al Dehydroepiandrosterone modulates nuclear factor-Kappa B activation in hippocampus of diabetic rats, Endocrinology 2002. 143:3250-3258
[25]Young IS, Woodside JV. Antioxidants in health and disease[J]. J ClinPathol, 2001, 54(3):176-186.
[26]Coppey LJ, Gellett JS , Davidson EP, et al. Effect of antioxidant treatment of streptozotocin-induced diabetic rats on endoneurial blood flow , motor nerve conduation velocity. and vascular reactivity of epineurial arterioles of the sciatic nerve . Diabetes. 2001. 50:1927-1937.
[27]Maritim AC, Sanders RA, Watkins JB 3rd. Effects of a-lipoic acid on biomarkers of oxidative stress in streptozotocin-induced diabetic rats. JNutr Biochem, 2003, 14:288-294
[28]Haak E, Usadel KH, Kusterer K, et al . Effects of a-lipoic acid on microcirculation in patients with peripheral diabetic neuropathy . Exp Clin Endocrinol Diabetes, 2000, 108:168-174.
[29]Stevens MJ, ObrosovaI, Cao X , et al . Effects of DL-alpha-lipic acid on Peripheral nerve conduction , blood flow , energy metabolism and oxidative Stress in experimental diabetic neuropathy. Diabetes, 2000, 49:1006-1015.
[30]AndroneL, Gavan NA, Veresiu IA, et al. In vivo effect of lipoic acid on Lipid peroxidation in patients with diabetic neuropathy. In Vivo, 2000, 14:327-330.
[31]El Midaoui A, de Champlain J. Prevention of hypertension , insulin resistance , and oxidativestress by alpha-lipoic-acid. Hypertention, 2002, 39:3032307.
[32]Coppey LJ , Gellett JS, Davidson EP, et al. Preventing superoxide for maTion in epineuria alarterioles of the sciatic nerve from diabetic rat srestores endothelium- dependent vasodilation. Free Radic Res, 2003, 37:33240.
[33]Stevens MJ, ObrosovaI , Cao X, et al. Effects of DL-alpha-lipic acid on peripheral nerve conduction, blood flow, energy metablism, and oxidative stress in experimental diabetic neuropathy. Diabetes, 2000, 49:1006-1015.
[34]Ametov AS , Barinov A, Dyck PJ, et al. The sensory symptoms of diabetic Polyneuropathy are improved with a-lipoic acid: The SYDNEY Trial. Diabetes Care, 2003, 26:770-776.
[35]Hahm JR, Kim BJ, Kim KW. Clinical experience with thioct acid(thioctic acid)in the treatment of distal symmetric polyneuropathy in Korean diabetic patient . J Diabetes Complications, 2004, 18:79285.
[36]Ziegler D , Nowak H, Kempler P, et al. Treatment of symptomatic diabetic Polyneuropathy with the antioxidant a-lipoic acid: a meta-analysis. DiabetMed, 2004, 21:1142121.
[37]Yorek MA, Coppey LJ, Gellett JS , et al. Effect of fidarestat and alpha-Lipoic acid on diabetes-induced epineurial arteriole vascular dysfunction. Exp Diabesity Res, 2004, 5:123-135.
[38]BAYDAS G, CANATAN H, TURKOGLU A. Comparative analys is of the protective effects of melatonin and vitaminE on streptozo-ci n-induced diabetes mellitus[J]. JPinealRes, 2002, 32(4):225-230.
[39]AKSOYN, VURALH, SABUNCUT, et al. Effect so fmelatonin on oxidative- antioxidative status of tissues in streptozotocin-induced diabetic rats[J]. CellBiochemFunct, 2003, 21(2):121-125.
[40]ANWAR M M, MEKIM. A. Oxidative stress in streptozotocin-induced diabetic rats: effects of garlic oil and melatonin[J]. CompBiochemPhysiol, 2003, 135(4):539-547.
[41]Aksoy N, Vural H, Sabuncu T, et al . Effects of melatonin on oxidative-antioxidative status of tissues in streptozotocin-induced diabetic rats . Cell Biochem Funct, 2003, 21:121-125.
[42]Wada R, Nishizawa Y, Yagihashi N, et al. Effects of OPB-9195, anti-glyCation agent, on experimental diabetic neuropathy. EurJClinInvest, 2001, 31:5132520.
[43]Chen AS, Taguchi T , Sugiura M , et al. Pyridoxal-aminoguanidine adduct is more effective than aminoguanidine in preventing neuropathy and cata-ract in diabetic rats. Horm Metab Res, 2004, 36:1832187.
[44]Cruz-Aguado R, Turner LF, Diaz CM, et al. Nerve growth factor and striatal glutathione metabolism in a rat model of Huntingtoncs’s disease [J]Restor Neurol Neurosci, 2000, 17(4):2172221.
[45]Huang TJ, Sayers NM , Verkhratsky A, et al. Neurotrophin-3 prevents mitochondrial dysfunction in sensory neurons of strep-tozotocin-diabetic rats [J]. ExpNeurol, 2005, 194(1):279-283.
[46]Kato N , Nemoto K , Nakanishi K, et al . Nonviral gene transfer of human hepatocyte growth factor improves streptozotocin-in-duced diabetic neuropathy in rats[ J]. Diabetes, 2005, 54(3):846-854.
[47]Li F ,Obrosova IG, Abatan O, et al .Taurine replacement attenu-ates hyperalgesia and abnormal calcium signaling in sensory neu-rons of STZ-D rats[J].Am J Physiol Endocrinol Metab,2005,288(1):29-36.
[48]程赣萍,王桥根,应卫婵.依达拉奉治疗糖尿病周围神经病变临床观察[J].中国药物与临床, 2006, 6(1):74-75.
[49]Young IS, Wood side JV. Antioxidants in health and disease [J]. J Clin Pathol, 2001, 54(3):1762186.
[2] Vinik AI, Vinik E. Prevention of the complications of dia betes. Am J Manag Care 2003;9(3Suppl):S63-80;quizS 81-84
[3]张春芳,钟历勇,张在强. 2型糖尿病严重周围神经病变1例[J].中国疼痛医学杂志, 2006, 12(4):201~202
[4] BRIL V, WERB MR, GREENE DA, et al, Single fiber Electromyo grapy in Diaetic Peripheral Polyneuropthy[J]. MusecleNerve(S0148-639X), 1996, 19(1):2-9
[5] Brownlee M. The pathobiology of diabetic complications:a unifying mechanism. Diabetes 2005;54:1615-1625.
[6] Feldman EL. Oxidative stress and diabetic neuropathy:a new understanding of an old problem[J]. JClinInvest, 2003, 111(4):431 -433.
[7] van DAMPS. Oxidative stress and diabetic neuropathy: pathophys-Iological mechanisms and treatment perspectives[J]. Diabetes Metab ResRev, 2002, 18(3):176-184.
[8] Nair N, Bedwal S, Prasad S, et al. Short term zinc deficiency in diet induces increased oxidative stress in testes and epididymis of rats[J]. Indian J exp Biol, 2005, 43(9):786 -794.
[9] Lal MA, KomerA, MatsuoY, etal. Combined antioxidant and COMT inhibitor treatment reverses renal abnorinalities in diabetic rats[J]. Diabetes,Aug 2000;49:1381-1389
[10]Kasuga M. Insulin resistance and pancreatic B cell failure. J CliInvest, 2006; 116:1756-1760
[11]裴凌鹏,金宗濂.葛根黄酮改善小鼠抗氧化功能的研究[J].营养学报, 2005, 26(6):505-506
[12]张文清,海春旭,龚书明.锌对大鼠肝微粒体脂质过氧化模型抗氧化作用的实验研究[J].解放军预防医学杂志, 1998, 16(4):250- 254.
[13]Grawel S, Wardas M, Niedworok E, et al. Malondialdehyde(MDA)as a lipid peroxidation marker[J]. Wiad Lek, 2004, 57(9-10):453- 455.
[14]Ceriello A, Bortolotti N, Falleti E, Taboga C, Tonutti L, Crescentini A, Motz E, Lizzio S, Russo A, Bartoli E 1997 Total radical-trapping antioxidant parameter in NIDDM patients. Diabetes Care 20:194–197
[15]Han XY, Xu ZR, Wang YZ, et al?Effect of cadm ium on Lipid peroxdation and activites of antioxdant enzymes in growing pigs?biolTiace Elem Res 2006;110 30):251-263
[16]Blaszczak R, Kujawski K, Kedziora Kornatowska K, etal The total antioxidant capacity and low-molecular antioxidant concentration in plasma of type 2 diabetes patients with different stage of metabolic compensation and concomitant diabetic nephropathy[J]. PolMerkuriuszLek, 2005, 18(103):29 -32.
[17]Bailey MA, Sanat KS A novel anti-oxidant and anti-cancer strategy:a peptoid anti-inflammatory drug conjugate with SOD mimic activity〔J〕. Biochem Biophys Res Commun, 2004;317(4):1155- 8.
[18]Hartnett ME , Stratton RD, Browne RW, et al. Serum markers of ox idative stress and severitv of diabetic retinopathy. Diabetes Care2000, 23(2):234-40.
[19]栾松,崔丽英,汤晓芙,等.糖尿病周围神经病运动神经传导速度分布.中华神经科杂志, 2006, 39(7):436 -439.
[20]吕文山,董砚虎,钱荣立.糖尿病的诊断与分型[J].中国糖尿病杂志, 2000, 8(1): 60-61
[21]苏雅茹,杨莉芹,张云云,等.血糖控制与糖尿病多发性神经病变患者临床表现的观察[J].中国糖尿病杂志, 2003, 11(6):442 -443.
[22].奉水东,凌宏艳?高果糖饲料诱导SD大鼠氧化应激及对其脂质代谢的影响?中华慢性病预防与控制?2005?13(1):7-8
[23]刘志民,孙亮亮氧化应激在糖尿病发病机制中作用的认识?内科理论与实践2007年第2卷第3期?153-155
[24]陈瑗,周玫.自由基医学基础与病理生理[M].北京:人民卫生出版社, 2002. 24-25.
[25]Nagley P, Zhang C, Lim ML, et al. Mitochondrial DNA deletions parallel age-linked decline in rat sensory nerve function[J]. Neurobiol Aging, 2001, 22(4):635-43.
[26]Fridlyand LE, Philipson LH. Does the glucose dependent insulin secretion mechanism itself cause oxidative stress in pancreaticβ3/Ccells?[J]. Diabetes, 2004, 53(8):1942 -1948.
[27]Kadenbach B. Intrinsic and extrinsic uncoupling of oxidative phosphory-lation[J]. Biochim. Biophys Acta, 2003, 1604(2):77-94.
[28]Guzik TJ, Mussa S, Gastaldi D, et al. Mechanisms of increased vascular superoxide production in human diabetes mellitus:role of NAD(P)H oxidase and endothelial nitric oxide synthase[J]. Circulation, 2002, 105(14):1656-1662.
[29]Ergul A, Schultz Johansen J, Stromhaug C, et al. Vascular dysfunction of venous bypass conduits is mediated by reactive oxygen species in diabetes:Role of endothelin-1 [J]. J Pharmacol Exp Ther, 2004, 313:70-77.
[30]Green K, Brand MD, Murphy MP. Prevention of mitochondrial oxidative damage as a therapeutic strategy in diabetes[J]. Diabetes, 2004, 53(Suppl1):S110-118.
[31]MARITIM A C, SANDERS R A, WATKINS J B 3rd. Diabetes, oxidative stress, and antioxidants:a review[J]. J Biochem Mol Toxicol, 2003, 17(1):24-38.
[32]王忠山,邹冬辉,赵慧,等.短期糖尿病雄性大鼠前列腺自由基和抗氧化水平变化研究[J].中国病理生理杂志2003, 19(6):803-806.
[33]屈智慧,王旭渤,李永勇,等.糖尿病大鼠肾脏氧化应激的实验研究[J].中国实验诊断学, 2004, 8(2):193-195.
[34]Du Y, Miller CM, Kern TS. [J]. Free Radic BiolMed, 2003, 35(11):1491-1499
[35]Haskins K, Bradley B, Powers K, et al. Oxidative stress in type 1 diabetes[J]. Ann NY Acad Sci, 2003, 1005(11)43-54.
[36]Kowluru RA, Abbas SN, Odenbach S. [J]. J Diabetes Complications, 2004, 18(5):282-288
[37]Stentz FB, Umpierrez GE, Cuervo R, et al. Proinflamma tory cytokines, markers of cardiovascular risks, oxidative stress, and lipid peroxidation in patients with hyperglyce miccrises[J]. Diabetes, 2004, 53(8):2079-2086.
[38]Marra G, Cotroneo P, Pitocco D. [J]. Diabetes Care, 2002, 25(2):370-375
[39]Ceriello A , Mercuri F, Quagliaro L, et al. Detection of nitrotyrosine in the diabetic plasma: evidence of oxidative stress. Diabetologia, 2001, 44:834-838
[40]Nishikawa T, Sasahara T, Kiritoshi S, et al. Evaluation of urinary8- hydroxydeoxy-guanosine as a novel biomarker of macrovascular complications in type 2 diabetes[J]. DiabetesCare, 2003, 26(5):1507-1512.
[41]Nishikawa T, Sasahara T, Kiritoshi S, et al. [J]. Diabetes Care, 2003, 26:1507-1512
[42]Wu LL , Chiou CC , Chang PY, et, al. Urinary 8-OHDG:a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetic. Clin Chim Acta, 2004, 339:1-9
[43]Shin CS, Moon BS, Park KS, et al. Serum 8-hydroxy-guanine levels are Increased in diabetic patients [J]. Diabetes Care, 2001, 24(4):733-737.
[44]Nobecourt E J acquerminet S, Hansel B, et al. Defective antioxidative activity of small dense HDL3 particles in type 2 diabetes: relationship to elevated oxidative stress and hyperglycaemia. Diabetologia, 2005, 48:529-538
[45]Stentz FB, Umpierrez GE, Cuervo R, et al. [J]. Diabetes, 2004, 53(8):2079-2086.
[46]Ziegler MD, Sohr CG, Nourooz-Zzdeh J. Oxidative stress and antioxidant defence in relation to the severity of diabetic polyneuropathy and cardiovascular autonomic neuropathy . Diabetes Care, 2004, 27:2178-2183.
[47]于翰柳洁糖尿病周围神经病变的研究进展山西医药杂志2007年9月第36卷第9期731-734
[48]Mohanty P, Hamouda W, Garg R, et al. Glucose challenge stimulates reactive oxygen species(ROS)generation by leucocytes. J Clin Endocrinol Metab 2000;85(8)∶2970-2973.
[49]Mentink CJ, Kilhovd BK, Rondas- Colbers GJ, etal. Time course of specific AGEs during optimized glycaemic control in type 2 diabetes. Neth J Med 2006;64(1)∶10-16.
[50]Tsukahara H, Sekine K, Uchiyama M, et al. Formation of advanced glycosylation end products and oxidative stress in young patients with type 1 diabetes. Pediatr Res 2003;54 (3)∶419-424.
[51]Usui T, Shizuuchi S, Watanabe H, et al. Cytotoxicity and oxidative stress induced by the glyceraldehyde-relatedmaillard reaction products for HL-60cells. Biosci Biotechnol Biochem 2004;68(2)∶333-340.
[52]Wauter MP, Chappey O, Corda S, et al. Activation of NADPH oxidase by AGE links oxidant stress to altered gene expression via RAGE. Am Jphysiol Endocrinol Metab2001; 280(5)∶E685-E694.
[53]Cellek S, Qu W, Schmidt AM, et al. Synergistic action of advanced glycation end products and endogenous nitric oxide leads to neuronal apoptosis in vitro:a new insight into selective nitrergic neuropathy in diabetes. Diabetologia2004;47 (2)∶331-339
[54]Kubo E, Urakami T, Fatma N, et al. Polyol pathway dependent osmotic and oxidative stresses in aldose reductase-mediated apoptosis in human lens epithelial cells:role of AOP2, Biochem Biophys Res Commun2004;314(4)∶1050-1056.
[55]Oyama T, Miyasita Y, Watanabe H, et al. The role of polyolpathway in high glucose -induced endothelial cell damages. Diabe Res Clin Pract 2006;73(3)∶227-234.
[56]Takeshi Suzuki, Hiromi Sekido, Noriaki Kato, et al. Neurotrophin- 3 induced production of nerve growth factor is suppressed in Schwann cells exposed to high glucose:involvement of the polyolpathway. Neurochemistry2004;91(6)∶1430-1438.
[57]Song ZT, Fu DT, Chan YS, et al. Transgenic mice overex pressing aldose reductase inSchwann cells show more severe nerve conduction velocity deficit and oxidative stress under hyperglycemic stress. Mol Cell Neurosci 2003;23(4)∶638-647.
[58]Cosentino F, Eto M, De Paolis P, et al. High glucose causes Upregulation of cyclooxygenase -2 and alters prostanoid profile in human end othelialcells:role of protein kinase Candreactive oxygen species. Circulation2003;107(7)∶1017-1023.
[59]Toshiyo Sonta, Toyoshi Inoguchi, Hirotaka Tsubouchi, et al. Evalution of oxidative stress in diabetic animals by invivo electron spin resonance measurement-role of protein kinase C. Diab Res Clin Pract 2004;66(Suppl1)∶S109-S113.
[60]Pricci F, Leto G, Amadio L, et al. Oxidative stress in diabetes induced end othelial dysfunction in volvement of nitricoxide and protein kinase C. Free Radic Biol Med 2003;35(6)∶683-694.
[61]Koh DW, Dawson TM, Dawson VL, Mediation of cell death by poly(ADP ribose)polymerase -1. PharmacolRes2005;52(1)∶5-14.
[62]Obrosova IG, Mabley JG, Zsengeller Z, et al. Role for ni trosative stress in diabetic neuropathy:evidence from studies with a peroxynitrite decomposition catalyst. FASEB J 2005;19(3)∶401-403.
[63]Obrosova IG, Drel VR, Pacher P, et al. Oxidative -nitrosative stress and poly(ADP -Ribose)plymerase(PARP)activation in experiment al diabetic neuropathy:the relation is revisited. Diabetes2005;54(12)∶3435-3441.
[64]Fukunaga M, Miyata S, Liu BF, et al. Methylglyoxal induces apoptosis through activation of p38 MAPK in rat Schwanncells. Biochem Biophy Res Commun2004; 320(3)∶689-695.
[65]Vincent AM, McLean LL, Backus C, et al. Short-termhy perglyce mia produces oxidative damage and apoptosis in neurons. FASEBJ2005;19(6)∶638-640.
[66]朱禧星.现代糖尿病学[M].第1版.上海:上海医科大学出版社, 2000. 318 -319.
[67]L lewelyn JG. . The diabetic neuropathies: types, diagnosis and management[J]. Jneurol Neurosurg Psychiatry, 2003, 74∶5-7.
[68]Gill GV, Huddle KR, MonkoeG. Longterm(20years)outcome and mortality of type1 diabeteic patients in Soweto, SouthAfrica. Diabet Med, 2005, 22:1642 -1646.
[69]王桂侠,李广仁,刘立,等.糖尿病周围神经病变患者神经病变及临床研究[J].中风与神经疾病杂志, 2000, 17(5):277
[70]李素梅,叶山东,杨静,等. 2型糖尿病周围神经传导速度与病程及尿清蛋白的关系---附66例报告[J].新医学, 1999, 30(6):327 328.
[71]史轶蔡.协和内分泌和代谢学[M]. 2版北京:科学出版社2000: 1339 - 40.
[72]王薇彬.等2型糖尿病患者氧化应激状况及影响因素分析[J]中国糖尿病杂志2007年第15卷第8期, 487-489
[73]史玉泉.实用神经病学[M].第2版.上海:上海科技出版社, 1994. 986-989.
[1] Feldman EL. Oxidative stress and diabetic neuropathy :a new under-Standing of an old problem [J]. JClinInvest, 2003, 111(4):431-433.
[2]陈瑗,周玫.自由基医学基础与病理生理[M].第1版.北京:人民卫生出版社, 2002.
[3] Wu LL , Chiou CC, ChangPY, etal. Urinary8-OHdG:amarkerofoxida-Tive stress to DNA and a risk factor for cancer, atherosclerosis and dia-betics. ClinChimActa, 2004, 339:1-9.
[4] Sharma A, Kharb S , Chugh SN, et al. Evaluation of oxidative stress before and after control of glycemia and after vitaminE supplementation in diabetic patients. Metabolism, 2000, 49:160-162.
[5] Bonnefont RD , Bastard JP, Jaudon MC , et al . Consequences of the diabetic status on the oxidant/antioxidant balance . Diabetes Metab, 2000, 26:1632176.
[6] Du Y, Miller CM, Kern TS. [J]. Free Radic BiolMed, 2003, 35(11):1491-1499
[7] Hoeldtke RD , Bryner KD, McNeill DR, et al. [J]. Diabetes Care, 2002, 25:1439-1443。
[8] Kanauchi M, Nishioka H, Hashimoto T. [J] . Nephron, 2002, 91;327-329
[9] Nishikawa T, Sasahara T, Kiritoshi S, et al. [J]. Diabetes Care, 2003, 26:1507-1512
[10]Kowluru RA, Abbas SN, Odenbach S. [J]. J Diabetes Complications, 2004, 18(5):282-288
[11]Shin CS, Moon BS, Park KS, et al. Serum 8-hydroxy-guanine levels are Increased in diabetic patients [J]. Diabetes Care, 2001, 24(4):733-737.
[12]Nobecourt E J acquerminet S, Hansel B, et al. Defective antioxidative activity of small dense HDL3 particles in type 2 diabetes: relationship to elevated oxidative stress and hyperglycaemia. Diabetologia, 2005, 48:529-538
[13]Schmeichel AM, Schmelzer JD , Low PA. Oxidative injury and apoptosis of doesal root ganglion neurons in chronic experimental diabetic neuropathy , Diabetes, 2003, 52:165-171.
[14]Coppey LJ, Gellet JS, Davidson EP, et al . Effect of treating treptozotocin induced diabetic rats with sorbinil, myo-inositol oraminoguanidine on endoneurial blood flow, motor nerve conduction velocity and vascular function of epineurial arterioles of the sciatic nerve. Int J Exp Diabetes Res, 2002, 3:21-36.
[15]Wang Y, Schmeichel AM, Lida H, et al . Ischemia-reperfusion injury causes oxidativestress and apoptosis of Schwann cell in acute and chronic experimental diabetic neuropathy. Antioxid Redox Signal, 2005, 7;1513-1520.
[16]Feldman EL. Oxidative stress and diabetic neuropathy :a new understanding of an old problem . J Clin Invest, 2003, 111:434-433.
[17]Wautier MP, Chappey O, Corda S, et al. Activation of NAPDH oxidase by AGE Links oxidant stress to altered gene expression via RAGE. Am J Physiol , 2001, 280:685-694.
[18]Sekido h, Suzuki T, Jomori T, et al . Reduced cell replication and induction of apoptosis by advanced glycation end products in rat Schwarm cells, Biochem Biophys Res Commun, 2004, 320:241-248.
[19]Cellek S, Qu W , Schmidt AM , et al , Synergistic action of advanced glycation end products and endogenous nitric oxide leads to neuronal apoptpsis in votro: a new insight into selective nitrergic neuropathy in diabetes. Diabetologia, 2004. 47:331-339.
[20]Song Z, Fu DT, Chan YS, et al Transgenic mice over expressing aldose reductase in Schwann cells show more severe nerve conduction velocity deficit and oxidative stress under hyperglycemic stress, Mol Cell Neurosci, 2003, 23:638-647.
[21]Babior BM, NADPH oxidase Curr Opin Immunol . 2004. 16:42-47
[22]Oh-Hashi KMaruyama W, Isobe KP eroxynitrite induces GADD34, 45. and 153 VLA p38 MAPK in human neuroblastoma SH-SY5Y cells, Free Radic Biol Med, 2001, 30;213-221.
[23]Rosen P . Nawroth PP, King G, et al . The role of oxidative stress in the onset and progression of diabetes and its complications:a summary of a Congress Series sponsored by UNESCO-MCBN, the American Diabetes Association and the German Diabetes Society . Diabetes Metab Res Rev , 2001. 17:189-212.
[24]Aragno M. Mastrocola R, Bringnardello E , et al Dehydroepiandrosterone modulates nuclear factor-Kappa B activation in hippocampus of diabetic rats, Endocrinology 2002. 143:3250-3258
[25]Young IS, Woodside JV. Antioxidants in health and disease[J]. J ClinPathol, 2001, 54(3):176-186.
[26]Coppey LJ, Gellett JS , Davidson EP, et al. Effect of antioxidant treatment of streptozotocin-induced diabetic rats on endoneurial blood flow , motor nerve conduation velocity. and vascular reactivity of epineurial arterioles of the sciatic nerve . Diabetes. 2001. 50:1927-1937.
[27]Maritim AC, Sanders RA, Watkins JB 3rd. Effects of a-lipoic acid on biomarkers of oxidative stress in streptozotocin-induced diabetic rats. JNutr Biochem, 2003, 14:288-294
[28]Haak E, Usadel KH, Kusterer K, et al . Effects of a-lipoic acid on microcirculation in patients with peripheral diabetic neuropathy . Exp Clin Endocrinol Diabetes, 2000, 108:168-174.
[29]Stevens MJ, ObrosovaI, Cao X , et al . Effects of DL-alpha-lipic acid on Peripheral nerve conduction , blood flow , energy metabolism and oxidative Stress in experimental diabetic neuropathy. Diabetes, 2000, 49:1006-1015.
[30]AndroneL, Gavan NA, Veresiu IA, et al. In vivo effect of lipoic acid on Lipid peroxidation in patients with diabetic neuropathy. In Vivo, 2000, 14:327-330.
[31]El Midaoui A, de Champlain J. Prevention of hypertension , insulin resistance , and oxidativestress by alpha-lipoic-acid. Hypertention, 2002, 39:3032307.
[32]Coppey LJ , Gellett JS, Davidson EP, et al. Preventing superoxide for maTion in epineuria alarterioles of the sciatic nerve from diabetic rat srestores endothelium- dependent vasodilation. Free Radic Res, 2003, 37:33240.
[33]Stevens MJ, ObrosovaI , Cao X, et al. Effects of DL-alpha-lipic acid on peripheral nerve conduction, blood flow, energy metablism, and oxidative stress in experimental diabetic neuropathy. Diabetes, 2000, 49:1006-1015.
[34]Ametov AS , Barinov A, Dyck PJ, et al. The sensory symptoms of diabetic Polyneuropathy are improved with a-lipoic acid: The SYDNEY Trial. Diabetes Care, 2003, 26:770-776.
[35]Hahm JR, Kim BJ, Kim KW. Clinical experience with thioct acid(thioctic acid)in the treatment of distal symmetric polyneuropathy in Korean diabetic patient . J Diabetes Complications, 2004, 18:79285.
[36]Ziegler D , Nowak H, Kempler P, et al. Treatment of symptomatic diabetic Polyneuropathy with the antioxidant a-lipoic acid: a meta-analysis. DiabetMed, 2004, 21:1142121.
[37]Yorek MA, Coppey LJ, Gellett JS , et al. Effect of fidarestat and alpha-Lipoic acid on diabetes-induced epineurial arteriole vascular dysfunction. Exp Diabesity Res, 2004, 5:123-135.
[38]BAYDAS G, CANATAN H, TURKOGLU A. Comparative analys is of the protective effects of melatonin and vitaminE on streptozo-ci n-induced diabetes mellitus[J]. JPinealRes, 2002, 32(4):225-230.
[39]AKSOYN, VURALH, SABUNCUT, et al. Effect so fmelatonin on oxidative- antioxidative status of tissues in streptozotocin-induced diabetic rats[J]. CellBiochemFunct, 2003, 21(2):121-125.
[40]ANWAR M M, MEKIM. A. Oxidative stress in streptozotocin-induced diabetic rats: effects of garlic oil and melatonin[J]. CompBiochemPhysiol, 2003, 135(4):539-547.
[41]Aksoy N, Vural H, Sabuncu T, et al . Effects of melatonin on oxidative-antioxidative status of tissues in streptozotocin-induced diabetic rats . Cell Biochem Funct, 2003, 21:121-125.
[42]Wada R, Nishizawa Y, Yagihashi N, et al. Effects of OPB-9195, anti-glyCation agent, on experimental diabetic neuropathy. EurJClinInvest, 2001, 31:5132520.
[43]Chen AS, Taguchi T , Sugiura M , et al. Pyridoxal-aminoguanidine adduct is more effective than aminoguanidine in preventing neuropathy and cata-ract in diabetic rats. Horm Metab Res, 2004, 36:1832187.
[44]Cruz-Aguado R, Turner LF, Diaz CM, et al. Nerve growth factor and striatal glutathione metabolism in a rat model of Huntingtoncs’s disease [J]Restor Neurol Neurosci, 2000, 17(4):2172221.
[45]Huang TJ, Sayers NM , Verkhratsky A, et al. Neurotrophin-3 prevents mitochondrial dysfunction in sensory neurons of strep-tozotocin-diabetic rats [J]. ExpNeurol, 2005, 194(1):279-283.
[46]Kato N , Nemoto K , Nakanishi K, et al . Nonviral gene transfer of human hepatocyte growth factor improves streptozotocin-in-duced diabetic neuropathy in rats[ J]. Diabetes, 2005, 54(3):846-854.
[47]Li F ,Obrosova IG, Abatan O, et al .Taurine replacement attenu-ates hyperalgesia and abnormal calcium signaling in sensory neu-rons of STZ-D rats[J].Am J Physiol Endocrinol Metab,2005,288(1):29-36.
[48]程赣萍,王桥根,应卫婵.依达拉奉治疗糖尿病周围神经病变临床观察[J].中国药物与临床, 2006, 6(1):74-75.
[49]Young IS, Wood side JV. Antioxidants in health and disease [J]. J Clin Pathol, 2001, 54(3):1762186.