两种赤潮藻在有机磷农药作用下的毒物刺激效应
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摘要
为了进一步认识有机磷农药污染对海洋赤潮藻的毒物刺激效应规律,深入了解有机磷农药残留是否构成引起海洋赤潮发生的因素,实验设置了0、0.001、0.01、0.1、1和10 mg·L-16个质量浓度梯度,研究了草甘膦异丙胺盐和敌敌畏对典型赤潮藻球形棕囊藻(Phaeocystis globosa)和赤潮异弯藻(Heterosigma akashiwo)生长速率、色素含量、可溶性蛋白含量、SOD和CAT活性以及营养吸收的影响。结果表明：(1)低浓度的草甘膦异丙胺盐和敌敌畏可以促进球形棕囊藻和赤潮异弯藻的生长,高浓度时表现出不同程度的抑制效应。两种农药对两种微藻产生毒物刺激效应的作用浓度存在差异。赤潮异弯藻对于两种有机磷农药均较为敏感,而球形棕囊藻对于两种有机磷农药的耐受性较强。(2)两种农药对海洋微藻的叶绿素a含量有显著影响,叶绿素a含量的变化趋势与藻细胞生长趋势一致,表现出在低浓度有机磷农药处理(<1 mg·L-1)下为促进效应,高浓度有机磷农药处理下藻体叶绿素a含量下降。(3)球形棕囊藻和赤潮异弯藻的抗氧化酶活性显著受两种有机磷农药浓度的影响,两种藻的SOD和CAT活性在草甘膦异丙胺盐作用下均呈现出低浓度促进和高浓度抑制的现象,并且SOD活性随着农药浓度增大逐渐降低,球形棕囊藻的CAT活性在0.001mg·L-1浓度处理下最高,赤潮异弯藻的CAT活性在0.1 mg·L-1浓度处理下最高；在敌敌畏作用下,两种微藻的SOD和CAT活性在24 h呈现出低浓度促进高浓度抑制的现象,但藻液培养到了48 h以后较高浓度(≥1 mg·L-1)敌敌畏处理组的抗氧化酶活性仍然超过对照组。(4)在低浓度有机磷农药草甘膦异丙胺盐和敌敌畏处理下,球形棕囊藻和赤潮异弯藻培养液中的无机磷和无机氮浓度均出现了显著降低,而在高浓度草甘膦异丙胺盐处理组,两种赤潮藻对培养液中磷酸盐和硝酸盐的消耗量均有显著减少。
Setting up 6 different mass concentrations 0,0.001,0.01,0.1,1 and 10 mg·L-1 of Glyphosate-isopropylammonium and DDVP, the growth rate, content of pigment, content of protein, activities of two antioxidant enzymes (SOD, CAT) and contents of inorganic P and N of red-tide species Phaeocystis globosa and Heterosigma akashiwo were measured in order to reveal the hormesis effect of organophosphorus pesticide in marine microalgae, and understand the laws of homesis induced by organophosphorus pesticides. The results showed:(1) Glyphosate-isopropylammonium and DDVP in low concentrations stimulated the growth of Phaeocystis globosa and Heterosigma akashiwo, while the growth of marine microalgae were restrained to different degrees under high concentrations of organophosphorus pesticides. There were significant concentrations and timing differences in the hormesis induced by organophosphorus pesticides in microalgae. Heterosigma akashiwo is more sensitive to two kinds of organophosphorus pesticides, and Phaeocystis globosa was tolerance stronger to organophosphorus pesticides. (2) Organophosphorus pesticides had significant impact on the content of chlorophyll-a. The variation trend of chlorophyll-a was consistent with the growth of microalgae, which showed inductive effect in low concentrations (<1 mg·L-1) while reduced the content of chlorophyll-a in high concentrations. (3) Antioxidant enzyme of Phaeocystis globosa and Heterosigma akashiwo were significantly affected by organophosphorus pesticides. Organophosphorus pesticides in low concentrations stimulated the SOD and CAT activities, while in high concentrations restrained the activities. Meanwhile, SOD activity decreased with the increasing concentration of pesticides. The highest CAT activity of Phaeocystis globosa was in the concentration of 0.001 mg·L-1 while Heterosigma akashiwo was in 0.1 mg·L-1. Under the treatments of DDVP, SOD and CAT activities of two kinds of microalgae showed hormesis effect in 24 hours, to 48 hours, the enzyme activities recovered at higher concentrations (≥1 mg·L-1). Antioxidant enzyme of Phaeocystis globosa and Heterosigma akashiwo were tolerance stronger to DDVP, and SOD was more sensitive to contamination. (4) Low concentrations of Glyphosate-isopropylammonium and DDVP accelerated the absorption of inorganic P and N. Meanwhile, there were higher inorganic P and N contents in the culture medium under higher concentrations of Glyphosate-isopropylammonium.
Setting up 6 different mass concentrations 0,0.001,0.01,0.1,1 and 10 mg·L-1 of Glyphosate-isopropylammonium and DDVP, the growth rate, content of pigment, content of protein, activities of two antioxidant enzymes (SOD, CAT) and contents of inorganic P and N of red-tide species Phaeocystis globosa and Heterosigma akashiwo were measured in order to reveal the hormesis effect of organophosphorus pesticide in marine microalgae, and understand the laws of homesis induced by organophosphorus pesticides. The results showed:(1) Glyphosate-isopropylammonium and DDVP in low concentrations stimulated the growth of Phaeocystis globosa and Heterosigma akashiwo, while the growth of marine microalgae were restrained to different degrees under high concentrations of organophosphorus pesticides. There were significant concentrations and timing differences in the hormesis induced by organophosphorus pesticides in microalgae. Heterosigma akashiwo is more sensitive to two kinds of organophosphorus pesticides, and Phaeocystis globosa was tolerance stronger to organophosphorus pesticides. (2) Organophosphorus pesticides had significant impact on the content of chlorophyll-a. The variation trend of chlorophyll-a was consistent with the growth of microalgae, which showed inductive effect in low concentrations (<1 mg·L-1) while reduced the content of chlorophyll-a in high concentrations. (3) Antioxidant enzyme of Phaeocystis globosa and Heterosigma akashiwo were significantly affected by organophosphorus pesticides. Organophosphorus pesticides in low concentrations stimulated the SOD and CAT activities, while in high concentrations restrained the activities. Meanwhile, SOD activity decreased with the increasing concentration of pesticides. The highest CAT activity of Phaeocystis globosa was in the concentration of 0.001 mg·L-1 while Heterosigma akashiwo was in 0.1 mg·L-1. Under the treatments of DDVP, SOD and CAT activities of two kinds of microalgae showed hormesis effect in 24 hours, to 48 hours, the enzyme activities recovered at higher concentrations (≥1 mg·L-1). Antioxidant enzyme of Phaeocystis globosa and Heterosigma akashiwo were tolerance stronger to DDVP, and SOD was more sensitive to contamination. (4) Low concentrations of Glyphosate-isopropylammonium and DDVP accelerated the absorption of inorganic P and N. Meanwhile, there were higher inorganic P and N contents in the culture medium under higher concentrations of Glyphosate-isopropylammonium.
引文
[1]陈景莲.农药对环境和人体危害的探讨[J].干旱区资源与环境,1999,10：132-134.
[2]陈峰,姜悦.微藻生物技术[M].北京：中国轻工业出版社,1999：35.
[3]邓朝晖,袁峻峰,陈德辉,左本荣.三种除草剂对斜生栅藻生长的影响[J].上海师范大学学报：自然科学版,2000,29(1)：92-95.
[4]戴宇飞,郑玉新.毒理学中心法则的重新审视-毒物兴奋性剂量-反应关系及其对毒理学发展的影响[J].国外医学卫生学分册,2003,30(4)：246-249.
[5]杜清平,黄彩娜,贾晓珊.1,2,4-三氯苯对3中海洋微藻的毒性效应[J].生态环境,2007,16(2)：352-357.
[6]冯朝,唐学玺,曲良,董栋,王其翔.UV-B辐射增强和蒽对3种赤潮微藻生长的相互作用[J].海洋环境科学,2008,27(1)：51-53.
[7]郭雪雁,马义兵,李波.陆地生态系统中低剂量毒物刺激作用及拟合模型研究进展[J].生态学报,2009,29(8)：4408-4419.
[8]高玉荣.杀虫剂单甲脒对绿藻的毒性研究[J].环境科学学报,1995,15(2)：92-98.
[9]贺红武.有机磷农药的现状与发展趋势[J].世界农药,2008,20(6)：29-33,6.
[10]黄健,唐学玺,宫相忠,李永祺.低浓度毒物对海洋微藻生长刺激效应的初步研究[J].应用生态学报,2002,13(11)：1516-1518.
[11]黄周英,王重刚,左正宏,赵扬,陈奕欣.三丁基锡对文蛤鳃的抗氧化酶活性及脂质过氧化的影响[J].环境科学学报,2005,25(10)：1408-1413.
[12]胡先文,张新萍,岳霞丽,叶发兵,董元彦.甲磺隆对水华鱼腥藻的抑制效应[J].农药,2003,42(12)：39-40.
[13]胡先文,陈加荣,董元彦.3磺酰脲除草剂对水华鱼腥藻的生长效应[J].农药,2005,44(7)：326-330.
[14]韩博平,韩志国,付翔.藻类光合作用机理与模型[M].北京：科学出版社,2003：178.
[15]陆荣柱,金复生,陈自强.化学兴奋效应及其毒理学意义[J].工业卫生与职业病,2001,27(3)：180-183.
[16]吕庆,郑荣梁.干旱及活性氧引起小麦膜脂过氧化及脱酯化[J].中国科学：C辑,1996,26(1)：26-30.
[17]李卓娜，孟范平，李顺顺，姚瑞华．2，2′,4,4′一四溴联苯醚(BDE-47)对4种海洋微藻的急性毒性[J].生态毒理学报,2009,4(3)：435-439.
[18]李永玉,洪华生,王新红,洪丽玉,叶翠杏.厦门海域有机磷农药污染现状与来源分析[J].环境科学学报,2005,25(8)：1071-1077.
[19]李建宏,袁俊,倪霞,翁永萍.酚对极大螺旋藻生长的影响[J].水生生物学报,2001,25(3)：294-296.
[20]李虹莉,孙宗光,官正宇.水中重点有机磷农药的毛细管气色谱测定[J].化学分析计量,2001,10(1)：15-17.
[21]李慧敏,李玉华,武佃卫,杨忠山,吴玉梅,等.Cu2+对栅藻和鱼腥藻增殖的影响[J].安全与环境学报,2007,7(3)：13-16.
[22]刘维屏.农药环境化学[M],北京：化学工业出版社,2006：40-41.
[23]刘英秀,胡怡秀.毒理学默认量效关系模型的重新审视[J].国外医学卫生学分册,2008,35(5)：247-263.
[24]刘晓娟,李爱芬,段舜山.UV-B辐射对绿色巴夫藻生长及抗氧化酶的影响[J].海洋科学,2007,31(4)：48-52.
[25]刘赞,翁建华.低浓度有机磷萃取剂P204促进普通小球藻生长的研究[J].环境化学,1998,17(2)：120-126.
[26]林玉锁,龚瑞忠,朱忠林.农药与生态环境保护[M].北京：化学工业出版社,2000：64.
[27]马剑敏,蔡林林,靳同霞,李效宇.溴化1-己基-3-甲基咪唑对斜生栅藻抗氧化酶和脂膜过氧化的影响[J].武汉植物学研究,2008,26(5)：509-513.
[28]聂湘平,蓝崇钰,林里,黄铭洪.多氯联苯对蛋白核小球藻和斜生栅藻生长影响的研究[J].中山大学学报：自然科学版,2002,41(1)：68-71.
[29]欧晓明,雷满香,黄明智,王跃龙,王晓光,等.新型除草剂HNPC-C9908对小球藻生长的影响研究[J].农药学学报,2003,5(3)：16-23.
[30]沈宏,周培疆.环境有机污染物对藻类生长作用的研究进展[J].水生生物学报,2002,26(5)：529-535.
[31]沈国兴,严国安,余新,刘永定,宋立荣,等.萘及其衍生物对普通小球藻的毒性效应[J].水生生物学报,1999,23(5)：460-468.
[32]沈国兴,严国安,彭金良,严雪.农药对藻类的生态毒学研究Ⅱ[J].环境科学进展,1999,7(6)：131-140.
[33]田继远,唐学玺,于娟,王晨,刘文明.蒽胁迫对2种海洋微藻的毒性效应[J].青岛海洋大学学报,2002,32(6)：919-925.
[34]唐学玺,李永祺,黄健.对硫磷对扁藻和杜氏盐藻脂膜的过氧化与脱脂化伤害[J].海洋与湖沼,1999,30(3)：295-299.
[35]唐学玺,张培玉.蒽对黑鮶超氧化物歧化酶活性的影响[J].水产学报,2000,24(3)：217-220.
[36]魏子昌.湖北省水资源开发利用现状及保护问题浅论[J].水资源保护,1988,4：96-101.
[37]王丽平,郑丙辉,孟伟.重金属Cu对两种海洋微藻的毒性效应[J].海洋环境科学,2007,26(1)：6-9.
[38]王悠,唐学玺,李永祺,刘泳.低浓度蒽对两种海洋微藻生长的兴奋效应[J].应用生态学报,2002,13(3)：343-346.
[39]王建华,刘鸿先,徐同.超氧化物歧化酶(SOD)在植物逆境和衰老中的作用[J].植物生理学通讯,1989,11(1)：1-7.
[40]王艳华,袁峻峰,丁峰元,张锦平,汤琳,等.苯乙烯对4种藻类的毒性效应[J].上海师范大学学报：自然科学版,2004,33(1)：97-101.
[41]吴颖慧,蔡磊明,王捷,王颖.除草剂莠去津对7种藻类的生长抑制[J].农药,2007,46(1)：48-51.
[42]吴银宝,廖新俤,汪植三,陈杖榴,王耀满.恩诺沙星对雨生红球藻的生长抑制毒性测定[J].华南农业大学学报,2005,26(4)：99-101.
[43]吴晓霞,吴进才,金银根,董波,王荣生.除草剂对水生植物的生理生态效应[J].生态学报,2004,24(9)：2037-2042.
[44]徐炜.高效液相色谱法测定环境水体中8种农药残留量[J].环境与健康杂志,2000,17(6)：362-363.
[45]谢荣,唐学玺,李永祺,王悠.丙溴磷影响海洋微藻生长机理的初步研究[J].环境科学学报,2000,20(4)：473-477.
[46]夏世钧,孙金秀,白喜耕,等.农药毒理学[M].北京：化学工业出版社,2008.
[47]尹明泉.农药对地下水污染评价方法的探讨[J].山东地质情报,1992,4：19-23.
[48]岳文杰,王朝晖.氯氰菊酯对锥状丝氏藻的毒性效应[J].生态科学,2009,28(2)：123-127.
[49]于娟,唐学玺,杨震.蒽对二种海洋微藻抗氧化系统的影响[J].应用与环境生物学报,2001,7(4)：340-343.
[50]庄铁诚,张良亨,叶德新.厦门地区化肥、农药使用现状与环境污染[J].福建环境,1995,12(3)：25-26.
[51]张辉,刘广民,姜桂兰,唐杰.农药在土壤环境中迁移转化规律研究的现状与展望[J].世界地质,2000,19(2)：199-204,208.
[52]张一宾,孙晶.国内外有机磷农药的概况及对我国有机磷农药发展的看法[J].农药,1999,38(7)：1-3.
[53]张志良,瞿伟菁.植物生理学实验指导[M].北京：高等教育出版社,2003：67-69.
[54]张蕾,王修林,韩秀荣,祝陈坚,石晓勇,等.石油烃污染物对海洋浮游植物生长的影响—实验与模型[J].青岛海洋大学学报,2002,32(5)：804-810.
[55]Arinaga S, Nobuya K. Correlation of eosinophilia with clinical response in patients with advanced carcinoma treated with low-dose recombinant interleukin-2 and mitomycin C [J]. Cancer Immunology Immunotherapy,1992,35:246-50.
[56]Calabrese E J. Toxicological awakenings:the rebirth of hormesis as a central pillar of toxicology [J]. Toxicol Appl Pharmacol,2005,204:1281.
[57]Calabrese E J. Cancer biology and hormesis:human tumor cell lines commonly display hormetic (biphasic) dose responses [J]. Critical reviews in toxicology,2005,35(6): 463-582.
[58]Calabrese E J. Hormetic dose-response relationships in immunology:occurrence, quantitative features of the dose response, mechanistic foundations, and clinical implications [J]. Critical reviews in toxicology,2005,35(2-3):89-295.
[59]Calabrese E J. Hormesis:from marginalization to mainstream:A case for hormesis as the default dose-response model in risk assessment [J]. Toxicology and Applied Pharmacology,2004,197(2):125-136.
[60]Calabrese E J. Overcompensation stimulation:a mechanism for hormetic effects [J]. Critical Reviews in Toxicology,2001,31:425-470.
[61]Calabrese E J, Baldwin L A. The hormetic dose-response model is more common than the threshold model in toxicology [J]. Toxicological science,2003,71:246-250.
[62]Calabrese E J, Baldwin L A. Peptides and hormesis [J]. Critical reviews in toxicology, 2003,33(3-4):355-405.
[63]Calabrese E J, Baldwin L A. Inorganics and hormesis [J]. Critical reviews in toxicology, 2003,33(3-4):215-304
[64]Calabrese E J, Baldwin L A. Chemotherapeutics and hormesis [J]. Critical reviews in toxicology,2003,33(3-4):305-353.
[65]Calabrese E J, Baldwin L A. Hormesis:a genealizable and unifying hypothesis [J]. Critical reviews in toxicology,2001,31(4-5):353-424.
[66]Calabrese E J, Baldwin L A. The frequency of U-shaped dose responses in the toxicological literature [J]. Toxicol Sci,2001,62:330-338.
[67]Calabrese E J, Baldwin L A. A general classification of U-shaped dose response relationships in toxicology and their mechanistic foundations [J]. Human and Experimental Toxicology,1998,17(7):353-364.
[68]Nina Cedergreen, Jens C. Streibig, Per Kudsk, Solvejg K. Mathiassen, Stephen O. Duke The occurrence of hormesis in plants and algae [J]. Dose-Response,2007,5:150-162.
[69]Banerjee B D, Seth V, Bhattarya A. Biochemical effects of some pesticides on lipid peroxidation and free radical scavengers [J]. Toxicology Letters,1999,107:33-48.
[70]Doggett S M, Rhodes R G. Effects of a diazinon formulation on unialgal growth rates and phytoplankton diversity [J]. Bull Environ Contam Toxicol,1991,47:36-42.
[71]Francesco Pomati, Andrew G. Netting, Davide Calamari, Brett A. Neilan. Effects of erythromycin, tetracycline and ibuprofen on the growth of Synechocystis sp. and Lemna minor [J]. Aquatic Toxicology,2004,67:387-396.
[72]Faust M, Altenburger R, Backhaus T, Blank H. Joint algal toxicity of 16 dissimilarly acting chemicals is predictable by the concept of independent action [J]. Aquatic Toxicology,2003,63:43-63.
[73]Hidalgo E, Dominguez C. Growth-altering effects of sodium hypochlorite in cultured human dermal fibroblasts [J]. Life Sciences,2002,67(11):1331-1344.
[74]Ikushima T, Aritomi H, Morisita J. Radioadaptive response:Efficient repair of radiation-induced DNA damage in adapted cells [J]. Mutatatiom research,1996,358: 193-198.
[75]Kaiser J. Hormesis:Sipping from a poisoned chalice [J]. Science,2003,302:376-379.
[76]Lorenzen C J. Determination of chlorophyll and pheo-pigments:spectrophotometric equations [J]. Limnology Oceanography,1967,12:343-346.
[77]Perona E. Alteration of dinitrogen fixing and metabolism in cyanobacterium Anabaena PCC7119 by phosphamidon [J]. Environ. And Botany,1991,31(4):479-488.
[78]Qian H F, Chen W, Li J, Wang J, Zhou Z, Liu W, et al. The effect of exogenous nitic oxide on alleviating herbicide damage in Chlorella vulgaris [J]. Aquatic Toxicology,2009, 92:250-257.
[79]R. Prado, R. Garcia, C. Rioboo, C. Herrero, J. Abalde, A. Cid. Comparison of the sensitivity of different toxicity test endpoints in a microalga exposed to the herbicide paraquat [J]. Environment International.2009:240-247.
[80]Stohs S T, Bagchi D. Oxidative mechanisms in the toxicity of metals [J]. Free Radical Biol Med,1995,18:321-326
[81]Motoharu SAKAUE, Seiichiroh OHSAKO, Ryuta ISHIMURA, Shuichi KUROSAWA, Masamichi KUROHMARU, et al. Bisphenol A affects spermatogenesis in the adult rat even at low doses [J]. Journal of Occupational Health,2001,43:185-190.
[82]Sergey M, Chemyak, Cliford P. Evidence of currently-used pesticides in air, ice, fog, seawater and surface microlayer in Bering and Chukchi Seas[J]. Marine Pollution Bulletin,1996,32(5):410-419.
[83]Schulz H. Uber Hefegifte [J]. Pflugers Archiv European Journal of Physiology,1888, 42(1):517-541.
[84]Sielken R, Stevenson D. Some implications for quantitative risk assessment if hormesis exists [J]. Human and Experimental Toxicology,1998,17:259-262.
[85]Southman C M, Ehrlich J. Effects of extracts of western red-cedar heartwood on certain wood-decaying fungi in culture [J]. Phytopathology,1943,33:517-524.
[86]Elizabeth T. Snow, Peter Sykora, Troy R. Durham, Catherine B. Klein. Arsenic, mode of action at biologically plausible low doses:What are the implications for low dose cancer risk [J]. Toxicology and Applied Pharmacology,2005,207(2S):557-564.
[87]Stebbing A R D. Growth hormesis:a by-product of control [J]. Health Physics,1987, 52(5):543-547. Stebbing A R D. Tolerance and hormesis-increased resistance to copper in hydroids linked to hormesis [J]. Marine Environmental Research,2002,54:805-809.
[88]Thayer K A, Melnick R, Burns K, Davis D, Huff J. Fundamental flaws of hormesis for public health decisions [J]. Environmental Health Perspectives,2005,113:1271-1276.
[89]Tian S Z, Liu Z, Weng J H, Zhang Y Y. Growth of Chlorella vulgaris in cultures with low concentration dimethoate as source of phosphorus [J]. Chemosphere,1997,35(11): 2713-2718.
[90]Tang X X, Li Y Q. Inhibition of antioxidants on monocrotophos damage to Platymonas sp. [J]. Environmental science,2000,21(1):87-89.
[91]Tripathi B N, Mehta S K, Amar A, Gaur J P. Oxidative stress in Scenedesmus sp. during short-and long-term exposure to Cu2+and Zn2+[J]. Chemosphere,2006,62:538-544.
[92]US EPA. Pesticides In Ground Water Database:A compilation of monitoring studies, 1971-1991 (Report EPA 734-12-92-001) [R]. Washington D C:US Environmental Protection Agency,1992.
[93]US EPA. National Survey of Pesticides in drinking water wells:Phase Ⅰ report (Report EPA 570/9-90-015) [R]. Washington D C:Environmental Protection Agency, Office of Pesticides and Toxic Substances,1990.
[94]Verbeke P, Clark B F C, Rattan S I S. Reduced levels of oxidized and glycoxidized proteins in human fibroblasts exposed to repeated mild heat shock during serial passaging in vitro [J]. Free Radical Biology and Medicine,2001,31(12):1593-1602.
[95]Zhou P K, Sui J L, Yang S H. Hormetic effect of low dose radiation on cellular DNA repair [J]. Chinese Journal of Radiological Medicine and Protection,1997,17(3): 155-158.
[96]Wauchope R D. The pesticide content of surface water draining from agricultural fields: A review [J]. Environ Qual,1978,7(4):459-472.
[97]Wong P K. Effects of 2,4-D, glyphosate and paraquat on growth, photosynthesis and chlorophyll-a synthesis of Scenedesmus quadricauda Berb 614 [J]. Chemosphere,2000, 41:177-182.
[98]Zhang Z, Dai M, Hong H. Dissolved insecticides and polychlorinated biphenyls in the Pearl River Estuary and South China Sea [J]. Environmental Monitoring,2002,4: 922-928.
[99]Zhou P J, Zheng Z H, Yu Z K, Qiu Y, Yan G, et al. Studies on the relationship between the growth of Chlorella vulgaris and phosphorus fractions in water of Lake Donghu, wuhan[J]. Acta Hydrobiologica Sinica,2001,25(6):571-576.
[2]陈峰,姜悦.微藻生物技术[M].北京：中国轻工业出版社,1999：35.
[3]邓朝晖,袁峻峰,陈德辉,左本荣.三种除草剂对斜生栅藻生长的影响[J].上海师范大学学报：自然科学版,2000,29(1)：92-95.
[4]戴宇飞,郑玉新.毒理学中心法则的重新审视-毒物兴奋性剂量-反应关系及其对毒理学发展的影响[J].国外医学卫生学分册,2003,30(4)：246-249.
[5]杜清平,黄彩娜,贾晓珊.1,2,4-三氯苯对3中海洋微藻的毒性效应[J].生态环境,2007,16(2)：352-357.
[6]冯朝,唐学玺,曲良,董栋,王其翔.UV-B辐射增强和蒽对3种赤潮微藻生长的相互作用[J].海洋环境科学,2008,27(1)：51-53.
[7]郭雪雁,马义兵,李波.陆地生态系统中低剂量毒物刺激作用及拟合模型研究进展[J].生态学报,2009,29(8)：4408-4419.
[8]高玉荣.杀虫剂单甲脒对绿藻的毒性研究[J].环境科学学报,1995,15(2)：92-98.
[9]贺红武.有机磷农药的现状与发展趋势[J].世界农药,2008,20(6)：29-33,6.
[10]黄健,唐学玺,宫相忠,李永祺.低浓度毒物对海洋微藻生长刺激效应的初步研究[J].应用生态学报,2002,13(11)：1516-1518.
[11]黄周英,王重刚,左正宏,赵扬,陈奕欣.三丁基锡对文蛤鳃的抗氧化酶活性及脂质过氧化的影响[J].环境科学学报,2005,25(10)：1408-1413.
[12]胡先文,张新萍,岳霞丽,叶发兵,董元彦.甲磺隆对水华鱼腥藻的抑制效应[J].农药,2003,42(12)：39-40.
[13]胡先文,陈加荣,董元彦.3磺酰脲除草剂对水华鱼腥藻的生长效应[J].农药,2005,44(7)：326-330.
[14]韩博平,韩志国,付翔.藻类光合作用机理与模型[M].北京：科学出版社,2003：178.
[15]陆荣柱,金复生,陈自强.化学兴奋效应及其毒理学意义[J].工业卫生与职业病,2001,27(3)：180-183.
[16]吕庆,郑荣梁.干旱及活性氧引起小麦膜脂过氧化及脱酯化[J].中国科学：C辑,1996,26(1)：26-30.
[17]李卓娜，孟范平，李顺顺，姚瑞华．2，2′,4,4′一四溴联苯醚(BDE-47)对4种海洋微藻的急性毒性[J].生态毒理学报,2009,4(3)：435-439.
[18]李永玉,洪华生,王新红,洪丽玉,叶翠杏.厦门海域有机磷农药污染现状与来源分析[J].环境科学学报,2005,25(8)：1071-1077.
[19]李建宏,袁俊,倪霞,翁永萍.酚对极大螺旋藻生长的影响[J].水生生物学报,2001,25(3)：294-296.
[20]李虹莉,孙宗光,官正宇.水中重点有机磷农药的毛细管气色谱测定[J].化学分析计量,2001,10(1)：15-17.
[21]李慧敏,李玉华,武佃卫,杨忠山,吴玉梅,等.Cu2+对栅藻和鱼腥藻增殖的影响[J].安全与环境学报,2007,7(3)：13-16.
[22]刘维屏.农药环境化学[M],北京：化学工业出版社,2006：40-41.
[23]刘英秀,胡怡秀.毒理学默认量效关系模型的重新审视[J].国外医学卫生学分册,2008,35(5)：247-263.
[24]刘晓娟,李爱芬,段舜山.UV-B辐射对绿色巴夫藻生长及抗氧化酶的影响[J].海洋科学,2007,31(4)：48-52.
[25]刘赞,翁建华.低浓度有机磷萃取剂P204促进普通小球藻生长的研究[J].环境化学,1998,17(2)：120-126.
[26]林玉锁,龚瑞忠,朱忠林.农药与生态环境保护[M].北京：化学工业出版社,2000：64.
[27]马剑敏,蔡林林,靳同霞,李效宇.溴化1-己基-3-甲基咪唑对斜生栅藻抗氧化酶和脂膜过氧化的影响[J].武汉植物学研究,2008,26(5)：509-513.
[28]聂湘平,蓝崇钰,林里,黄铭洪.多氯联苯对蛋白核小球藻和斜生栅藻生长影响的研究[J].中山大学学报：自然科学版,2002,41(1)：68-71.
[29]欧晓明,雷满香,黄明智,王跃龙,王晓光,等.新型除草剂HNPC-C9908对小球藻生长的影响研究[J].农药学学报,2003,5(3)：16-23.
[30]沈宏,周培疆.环境有机污染物对藻类生长作用的研究进展[J].水生生物学报,2002,26(5)：529-535.
[31]沈国兴,严国安,余新,刘永定,宋立荣,等.萘及其衍生物对普通小球藻的毒性效应[J].水生生物学报,1999,23(5)：460-468.
[32]沈国兴,严国安,彭金良,严雪.农药对藻类的生态毒学研究Ⅱ[J].环境科学进展,1999,7(6)：131-140.
[33]田继远,唐学玺,于娟,王晨,刘文明.蒽胁迫对2种海洋微藻的毒性效应[J].青岛海洋大学学报,2002,32(6)：919-925.
[34]唐学玺,李永祺,黄健.对硫磷对扁藻和杜氏盐藻脂膜的过氧化与脱脂化伤害[J].海洋与湖沼,1999,30(3)：295-299.
[35]唐学玺,张培玉.蒽对黑鮶超氧化物歧化酶活性的影响[J].水产学报,2000,24(3)：217-220.
[36]魏子昌.湖北省水资源开发利用现状及保护问题浅论[J].水资源保护,1988,4：96-101.
[37]王丽平,郑丙辉,孟伟.重金属Cu对两种海洋微藻的毒性效应[J].海洋环境科学,2007,26(1)：6-9.
[38]王悠,唐学玺,李永祺,刘泳.低浓度蒽对两种海洋微藻生长的兴奋效应[J].应用生态学报,2002,13(3)：343-346.
[39]王建华,刘鸿先,徐同.超氧化物歧化酶(SOD)在植物逆境和衰老中的作用[J].植物生理学通讯,1989,11(1)：1-7.
[40]王艳华,袁峻峰,丁峰元,张锦平,汤琳,等.苯乙烯对4种藻类的毒性效应[J].上海师范大学学报：自然科学版,2004,33(1)：97-101.
[41]吴颖慧,蔡磊明,王捷,王颖.除草剂莠去津对7种藻类的生长抑制[J].农药,2007,46(1)：48-51.
[42]吴银宝,廖新俤,汪植三,陈杖榴,王耀满.恩诺沙星对雨生红球藻的生长抑制毒性测定[J].华南农业大学学报,2005,26(4)：99-101.
[43]吴晓霞,吴进才,金银根,董波,王荣生.除草剂对水生植物的生理生态效应[J].生态学报,2004,24(9)：2037-2042.
[44]徐炜.高效液相色谱法测定环境水体中8种农药残留量[J].环境与健康杂志,2000,17(6)：362-363.
[45]谢荣,唐学玺,李永祺,王悠.丙溴磷影响海洋微藻生长机理的初步研究[J].环境科学学报,2000,20(4)：473-477.
[46]夏世钧,孙金秀,白喜耕,等.农药毒理学[M].北京：化学工业出版社,2008.
[47]尹明泉.农药对地下水污染评价方法的探讨[J].山东地质情报,1992,4：19-23.
[48]岳文杰,王朝晖.氯氰菊酯对锥状丝氏藻的毒性效应[J].生态科学,2009,28(2)：123-127.
[49]于娟,唐学玺,杨震.蒽对二种海洋微藻抗氧化系统的影响[J].应用与环境生物学报,2001,7(4)：340-343.
[50]庄铁诚,张良亨,叶德新.厦门地区化肥、农药使用现状与环境污染[J].福建环境,1995,12(3)：25-26.
[51]张辉,刘广民,姜桂兰,唐杰.农药在土壤环境中迁移转化规律研究的现状与展望[J].世界地质,2000,19(2)：199-204,208.
[52]张一宾,孙晶.国内外有机磷农药的概况及对我国有机磷农药发展的看法[J].农药,1999,38(7)：1-3.
[53]张志良,瞿伟菁.植物生理学实验指导[M].北京：高等教育出版社,2003：67-69.
[54]张蕾,王修林,韩秀荣,祝陈坚,石晓勇,等.石油烃污染物对海洋浮游植物生长的影响—实验与模型[J].青岛海洋大学学报,2002,32(5)：804-810.
[55]Arinaga S, Nobuya K. Correlation of eosinophilia with clinical response in patients with advanced carcinoma treated with low-dose recombinant interleukin-2 and mitomycin C [J]. Cancer Immunology Immunotherapy,1992,35:246-50.
[56]Calabrese E J. Toxicological awakenings:the rebirth of hormesis as a central pillar of toxicology [J]. Toxicol Appl Pharmacol,2005,204:1281.
[57]Calabrese E J. Cancer biology and hormesis:human tumor cell lines commonly display hormetic (biphasic) dose responses [J]. Critical reviews in toxicology,2005,35(6): 463-582.
[58]Calabrese E J. Hormetic dose-response relationships in immunology:occurrence, quantitative features of the dose response, mechanistic foundations, and clinical implications [J]. Critical reviews in toxicology,2005,35(2-3):89-295.
[59]Calabrese E J. Hormesis:from marginalization to mainstream:A case for hormesis as the default dose-response model in risk assessment [J]. Toxicology and Applied Pharmacology,2004,197(2):125-136.
[60]Calabrese E J. Overcompensation stimulation:a mechanism for hormetic effects [J]. Critical Reviews in Toxicology,2001,31:425-470.
[61]Calabrese E J, Baldwin L A. The hormetic dose-response model is more common than the threshold model in toxicology [J]. Toxicological science,2003,71:246-250.
[62]Calabrese E J, Baldwin L A. Peptides and hormesis [J]. Critical reviews in toxicology, 2003,33(3-4):355-405.
[63]Calabrese E J, Baldwin L A. Inorganics and hormesis [J]. Critical reviews in toxicology, 2003,33(3-4):215-304
[64]Calabrese E J, Baldwin L A. Chemotherapeutics and hormesis [J]. Critical reviews in toxicology,2003,33(3-4):305-353.
[65]Calabrese E J, Baldwin L A. Hormesis:a genealizable and unifying hypothesis [J]. Critical reviews in toxicology,2001,31(4-5):353-424.
[66]Calabrese E J, Baldwin L A. The frequency of U-shaped dose responses in the toxicological literature [J]. Toxicol Sci,2001,62:330-338.
[67]Calabrese E J, Baldwin L A. A general classification of U-shaped dose response relationships in toxicology and their mechanistic foundations [J]. Human and Experimental Toxicology,1998,17(7):353-364.
[68]Nina Cedergreen, Jens C. Streibig, Per Kudsk, Solvejg K. Mathiassen, Stephen O. Duke The occurrence of hormesis in plants and algae [J]. Dose-Response,2007,5:150-162.
[69]Banerjee B D, Seth V, Bhattarya A. Biochemical effects of some pesticides on lipid peroxidation and free radical scavengers [J]. Toxicology Letters,1999,107:33-48.
[70]Doggett S M, Rhodes R G. Effects of a diazinon formulation on unialgal growth rates and phytoplankton diversity [J]. Bull Environ Contam Toxicol,1991,47:36-42.
[71]Francesco Pomati, Andrew G. Netting, Davide Calamari, Brett A. Neilan. Effects of erythromycin, tetracycline and ibuprofen on the growth of Synechocystis sp. and Lemna minor [J]. Aquatic Toxicology,2004,67:387-396.
[72]Faust M, Altenburger R, Backhaus T, Blank H. Joint algal toxicity of 16 dissimilarly acting chemicals is predictable by the concept of independent action [J]. Aquatic Toxicology,2003,63:43-63.
[73]Hidalgo E, Dominguez C. Growth-altering effects of sodium hypochlorite in cultured human dermal fibroblasts [J]. Life Sciences,2002,67(11):1331-1344.
[74]Ikushima T, Aritomi H, Morisita J. Radioadaptive response:Efficient repair of radiation-induced DNA damage in adapted cells [J]. Mutatatiom research,1996,358: 193-198.
[75]Kaiser J. Hormesis:Sipping from a poisoned chalice [J]. Science,2003,302:376-379.
[76]Lorenzen C J. Determination of chlorophyll and pheo-pigments:spectrophotometric equations [J]. Limnology Oceanography,1967,12:343-346.
[77]Perona E. Alteration of dinitrogen fixing and metabolism in cyanobacterium Anabaena PCC7119 by phosphamidon [J]. Environ. And Botany,1991,31(4):479-488.
[78]Qian H F, Chen W, Li J, Wang J, Zhou Z, Liu W, et al. The effect of exogenous nitic oxide on alleviating herbicide damage in Chlorella vulgaris [J]. Aquatic Toxicology,2009, 92:250-257.
[79]R. Prado, R. Garcia, C. Rioboo, C. Herrero, J. Abalde, A. Cid. Comparison of the sensitivity of different toxicity test endpoints in a microalga exposed to the herbicide paraquat [J]. Environment International.2009:240-247.
[80]Stohs S T, Bagchi D. Oxidative mechanisms in the toxicity of metals [J]. Free Radical Biol Med,1995,18:321-326
[81]Motoharu SAKAUE, Seiichiroh OHSAKO, Ryuta ISHIMURA, Shuichi KUROSAWA, Masamichi KUROHMARU, et al. Bisphenol A affects spermatogenesis in the adult rat even at low doses [J]. Journal of Occupational Health,2001,43:185-190.
[82]Sergey M, Chemyak, Cliford P. Evidence of currently-used pesticides in air, ice, fog, seawater and surface microlayer in Bering and Chukchi Seas[J]. Marine Pollution Bulletin,1996,32(5):410-419.
[83]Schulz H. Uber Hefegifte [J]. Pflugers Archiv European Journal of Physiology,1888, 42(1):517-541.
[84]Sielken R, Stevenson D. Some implications for quantitative risk assessment if hormesis exists [J]. Human and Experimental Toxicology,1998,17:259-262.
[85]Southman C M, Ehrlich J. Effects of extracts of western red-cedar heartwood on certain wood-decaying fungi in culture [J]. Phytopathology,1943,33:517-524.
[86]Elizabeth T. Snow, Peter Sykora, Troy R. Durham, Catherine B. Klein. Arsenic, mode of action at biologically plausible low doses:What are the implications for low dose cancer risk [J]. Toxicology and Applied Pharmacology,2005,207(2S):557-564.
[87]Stebbing A R D. Growth hormesis:a by-product of control [J]. Health Physics,1987, 52(5):543-547. Stebbing A R D. Tolerance and hormesis-increased resistance to copper in hydroids linked to hormesis [J]. Marine Environmental Research,2002,54:805-809.
[88]Thayer K A, Melnick R, Burns K, Davis D, Huff J. Fundamental flaws of hormesis for public health decisions [J]. Environmental Health Perspectives,2005,113:1271-1276.
[89]Tian S Z, Liu Z, Weng J H, Zhang Y Y. Growth of Chlorella vulgaris in cultures with low concentration dimethoate as source of phosphorus [J]. Chemosphere,1997,35(11): 2713-2718.
[90]Tang X X, Li Y Q. Inhibition of antioxidants on monocrotophos damage to Platymonas sp. [J]. Environmental science,2000,21(1):87-89.
[91]Tripathi B N, Mehta S K, Amar A, Gaur J P. Oxidative stress in Scenedesmus sp. during short-and long-term exposure to Cu2+and Zn2+[J]. Chemosphere,2006,62:538-544.
[92]US EPA. Pesticides In Ground Water Database:A compilation of monitoring studies, 1971-1991 (Report EPA 734-12-92-001) [R]. Washington D C:US Environmental Protection Agency,1992.
[93]US EPA. National Survey of Pesticides in drinking water wells:Phase Ⅰ report (Report EPA 570/9-90-015) [R]. Washington D C:Environmental Protection Agency, Office of Pesticides and Toxic Substances,1990.
[94]Verbeke P, Clark B F C, Rattan S I S. Reduced levels of oxidized and glycoxidized proteins in human fibroblasts exposed to repeated mild heat shock during serial passaging in vitro [J]. Free Radical Biology and Medicine,2001,31(12):1593-1602.
[95]Zhou P K, Sui J L, Yang S H. Hormetic effect of low dose radiation on cellular DNA repair [J]. Chinese Journal of Radiological Medicine and Protection,1997,17(3): 155-158.
[96]Wauchope R D. The pesticide content of surface water draining from agricultural fields: A review [J]. Environ Qual,1978,7(4):459-472.
[97]Wong P K. Effects of 2,4-D, glyphosate and paraquat on growth, photosynthesis and chlorophyll-a synthesis of Scenedesmus quadricauda Berb 614 [J]. Chemosphere,2000, 41:177-182.
[98]Zhang Z, Dai M, Hong H. Dissolved insecticides and polychlorinated biphenyls in the Pearl River Estuary and South China Sea [J]. Environmental Monitoring,2002,4: 922-928.
[99]Zhou P J, Zheng Z H, Yu Z K, Qiu Y, Yan G, et al. Studies on the relationship between the growth of Chlorella vulgaris and phosphorus fractions in water of Lake Donghu, wuhan[J]. Acta Hydrobiologica Sinica,2001,25(6):571-576.