Paxillin、VEGF在胃癌组织中的表达及意义
摘要
目的：探讨Paxillin、VEGF在胃癌组织中的表达及其与胃癌临床病理特征之间的关系。
方法：采用免疫组织化学法检测Paxillin和VEGF在55例胃癌组织和20例正常胃切缘组织中的表达。
结果：(1)Paxillin蛋白在胃癌组织中阳性表达率为67.27%(37／55),在正常胃切缘组织中阳性表达率20.00%(4／20),两组间差异具有显著性(P<0.01)。(2)VEGF在胃癌组织中的阳性表达率为72.72%(40／55),在正常胃切缘组织中的阳性表达率为35.00%(7／20),两组间差异具有显著性(P<0.01)。(3)Paxillin阳性表达在不同肿瘤直径间、不同组织分化程度、不同胃癌浸润深度、不同TNM分期及是否有淋巴结转移间,差异均有统计学意义(P<0.05),而在不同性别、年龄间差异无统计学意义(均P>0.05)。(4)VEGF阳性表达在不同组织分化程度、不同胃癌浸润深度、不同TNM分期及是否有淋巴结转移间,差异均有统计学意义(P<0.05),而在不同性别、年龄及肿瘤直径间差异无统计学意义(均P>0.05)。(5)VEGF和Paxillin蛋白的表达存在正相关关系,相关系数r=0.460(P<0.01)。
结论：(1)Paxillin、VEGF在胃癌组织中呈高表达状态,并且两者的表达均与组织分化程度、胃癌浸润深度、TNM分期、淋巴结转移密切相关,提示它们参与了胃癌的发生发展和转移。(2)有区域淋巴结转移病例的Paxillin、VEGF阳性表达率显著高于无淋巴结转移的病例,提示二者表达的增高可促进胃癌淋巴结的转移,这对于胃癌手术的根治范围和术后化疗有一定的指导意义；(3)Paxillin与VEGF在胃癌组织中的表达呈正相关,提示二者协同参与了胃癌的发生发展和转移。
Objective:To investigate the expression of paxillin, VEGF and their clinical significance in gastric cancer.
Methods:Expression of paxillin and VEGF in 50 cases of gastric cancer and 20 normal gastric tissues was detected by immunohistochemistry staining.x2 and Fisher exact probability test were used to analyze the data, there was a statistical significance when P<0.05.
Results: The positive expression rate of paxillin in gastric cancer was 67.27%(37/55), significantly higher than that in the normal gastric tissues(20.00%, P< 0.05).The positive expression rate of VEGF in gastric cancer was 72.72%(40/55), significantly higher than that in the normal gastric tissues (35.00%, P<0.05). The expression of paxillin in gastric cancer was significantly correlated with the tumor size, Borrmann's type, the depth of invasion, TNM stages, and lymph node metastasis (P< 0.05).The expression of VEGF in gastric cancer was significantly correlated with Borrmann's type, the depth of invasion, TNM stages, lymph node metastasis, (P<0.05).There was a positive correlation between the expression of paxillin and VEGF, proved by the correlation test(r=0.460, P<0.01).
Conclusion:paxillin and VEGF in gastric cancer tissues showed high expression, indicating that they may promote the development of gastric cancer. They were the development and metastasis of gastric cancer. In a regional lymph node metastasis cases, paxillin and VEGF expression was significantly increased, indicating that they may promote the lymph node metastasis. Meanwhile, It could guide the scope of radical surgery for gastric cancer and postoperative chemotherapy. Paxillin and VEGF expression in gastric cancer tissues was positively correlated, suggesting that synergy between the two involved in the occurrence of gastric cancer development and metastasis.
方法：采用免疫组织化学法检测Paxillin和VEGF在55例胃癌组织和20例正常胃切缘组织中的表达。
结果：(1)Paxillin蛋白在胃癌组织中阳性表达率为67.27%(37／55),在正常胃切缘组织中阳性表达率20.00%(4／20),两组间差异具有显著性(P<0.01)。(2)VEGF在胃癌组织中的阳性表达率为72.72%(40／55),在正常胃切缘组织中的阳性表达率为35.00%(7／20),两组间差异具有显著性(P<0.01)。(3)Paxillin阳性表达在不同肿瘤直径间、不同组织分化程度、不同胃癌浸润深度、不同TNM分期及是否有淋巴结转移间,差异均有统计学意义(P<0.05),而在不同性别、年龄间差异无统计学意义(均P>0.05)。(4)VEGF阳性表达在不同组织分化程度、不同胃癌浸润深度、不同TNM分期及是否有淋巴结转移间,差异均有统计学意义(P<0.05),而在不同性别、年龄及肿瘤直径间差异无统计学意义(均P>0.05)。(5)VEGF和Paxillin蛋白的表达存在正相关关系,相关系数r=0.460(P<0.01)。
结论：(1)Paxillin、VEGF在胃癌组织中呈高表达状态,并且两者的表达均与组织分化程度、胃癌浸润深度、TNM分期、淋巴结转移密切相关,提示它们参与了胃癌的发生发展和转移。(2)有区域淋巴结转移病例的Paxillin、VEGF阳性表达率显著高于无淋巴结转移的病例,提示二者表达的增高可促进胃癌淋巴结的转移,这对于胃癌手术的根治范围和术后化疗有一定的指导意义；(3)Paxillin与VEGF在胃癌组织中的表达呈正相关,提示二者协同参与了胃癌的发生发展和转移。
Objective:To investigate the expression of paxillin, VEGF and their clinical significance in gastric cancer.
Methods:Expression of paxillin and VEGF in 50 cases of gastric cancer and 20 normal gastric tissues was detected by immunohistochemistry staining.x2 and Fisher exact probability test were used to analyze the data, there was a statistical significance when P<0.05.
Results: The positive expression rate of paxillin in gastric cancer was 67.27%(37/55), significantly higher than that in the normal gastric tissues(20.00%, P< 0.05).The positive expression rate of VEGF in gastric cancer was 72.72%(40/55), significantly higher than that in the normal gastric tissues (35.00%, P<0.05). The expression of paxillin in gastric cancer was significantly correlated with the tumor size, Borrmann's type, the depth of invasion, TNM stages, and lymph node metastasis (P< 0.05).The expression of VEGF in gastric cancer was significantly correlated with Borrmann's type, the depth of invasion, TNM stages, lymph node metastasis, (P<0.05).There was a positive correlation between the expression of paxillin and VEGF, proved by the correlation test(r=0.460, P<0.01).
Conclusion:paxillin and VEGF in gastric cancer tissues showed high expression, indicating that they may promote the development of gastric cancer. They were the development and metastasis of gastric cancer. In a regional lymph node metastasis cases, paxillin and VEGF expression was significantly increased, indicating that they may promote the lymph node metastasis. Meanwhile, It could guide the scope of radical surgery for gastric cancer and postoperative chemotherapy. Paxillin and VEGF expression in gastric cancer tissues was positively correlated, suggesting that synergy between the two involved in the occurrence of gastric cancer development and metastasis.
引文
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[2]Chen HY, Shen CH, Tsai YT, et al. Brk activates racl and promotescell migration and invasion by paxillin phosphorylating [J]. MolcellBiol,2004,24(24):10558-10572.
[3]Chen JQ, Zhan WH, He YL, et al. Expression of heparanasegene,CD44v6, MMP-7and nm23 protein and their relationship with the invasion and metastasis of gastric carcinoma[J]. World Gastroenterol,2004,15(10):776-782.
[4]王雷,杜媛鲲,张雷.Paxillin和CD44v6在食管癌中的表达及其意义.天津医[J].2007.35(10)：742-744.
[5]赖坤兰,邹继彬.Paxillin和Survivin在结肠癌中的表达及其意义.实用医技杂志[J].2008,15(11)：1404-1406.
[6]胡美漪.胃癌组织中VEGF和CD34的表达及微血管密度的临床病理意义[J].中国厂矿医学,2005,13(2)：97-99.
[7]Tse, V., et al., The temporal-spatial expression of VEGF, angiopoietins-1 and 2, and Tie-2 during tumor angiogenesis and their functional correlation with tumor neovascular architecture. Neurol Res,2003,25(7):p.729-738..
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[9]Kamiya K, Konno H, Tanaka T, et al.Antitumor effect on human gastric cancer and induction of apoptosis by vascular endothelial growth factor neutralizing antibody[J]. Jpn J Cancer Res,1999,90 (7):794-800.
[10]Buchler P,Reber HA,Buchler MW,etal. VEGF-RⅡ Influences the prognosis of pancreatic cancer[J]. Ann surg,2002,236(6):738-749.
[11]Wu, Y.M., et al., Morphological changes and molecular expressions of hepatocellular carcinoma cells in three-dimensional culture model. Exp Mol Pathol,2009.87(2):133-140.
[12]左万里,李海刚.肺癌桩蛋白与血管内皮生长因子的表达及其临床意义[J].中国实用内科杂志,2003,23(2)：76-77.
[13]Lu, C.X., et al., Sulfated polymannuroguluronate, a novel anti-AIDS drug candidate, inhibits HIV-1 Tat-induced angiogenesis in Kaposi's sarcoma cells[J]. Biochem Pharmacol, 2007,74(9):1330-1339.
[14]van Zyp, J.V, et al., Extracellular pressure stimulates tumor cell adhesion in vitro by paxillin activation [J]. Cancer Biol Ther,2006.5(9):p.1169-1178.
[15]翟建文,王巧梅,王延臣,等.埃兹蛋白的表达在食管鳞癌中的诊断价值[J].中国实 验诊断学,2008,12(8)：1014-1017.
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