“畜禽用多种微量元素注射液”在山羊体内的药效学及临床试验研究
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摘要
“畜禽用多种微量元素注射液”为多种微量元素根据平衡补给原理制成的针剂。为进一步给临床用药提供依据,本实验旨对“畜禽用多种微量元素注射液”进行药效学研究和临床试验研究,以观察其对山羊血液学指标、免疫功能的影响以及对贫血羔羊的临床治疗效果。
1.“畜禽用多种微量元素注射液”对羔羊血液学及生理生化指标影响20只2月龄羔羊,随机分为对照组和试验组,每组10只,给药前静脉采抗凝血作为空白对照。试验组与对照组每周肌肉注射畜禽用多种微量元素注射液与生理盐水0.8 mL,连续8周。前腔静脉采血,测定血液中的红细胞、白细胞、淋巴细胞的数量和血红蛋白的含量以及血清中过氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)含量。结果表明,山羊注射多种微量元素注射液后,可显著使其红细胞、白细胞、淋巴细胞的数量及血红蛋白的含量升高(P<0.05);可使山羊血清中SOD的活性显著升高(P<0.05),使MDA的含量显著降低(P<0.05),使GSH-Px的活性显著升高(P<0.05)。
2.“畜禽用多种微量元素注射液”对羔羊免疫功能及增重的影响通过E玫瑰花环试验和淋巴细胞转化增殖试验研究了“畜禽用多种微量元素注射液”对羔羊免疫功能的影响。动物分组与处理同前。给药后每隔15 d静脉采血一次,抗凝处理,分离外周血淋巴细胞,进行E-玫瑰花环试验和淋巴细胞转化增殖试验。试验前和试验后1个月、2个月分别称量体重一次,进行增重试验。试验结果表明,使用多种微量元素注射液的实验组的E-玫瑰花环形成率和淋巴细胞的转化率显著高于对照组(P<0.05);同时对羔羊有显著的促生长作用(P<0.01)。
3.“畜禽用多种微量元素注射液”对小鼠免疫功能的影响将60只昆明小鼠(体重为20±2 g,雌雄各半)随机分为3组,每组20只。再将每组的20只小鼠分成试验组和对照组,每组10只,试验组每天腹腔注射0.04 mL“多种微量元素注射液”,对照组注射0.04 mL生理盐水,连续注射7 d。三组分别进行酸性α-醋酸萘脂酶法试验,溶血空斑试验和血清溶血素抗体(HC50)测定。结果显示,试验组小鼠ANAE阳性细胞百分比为51.80%±3.645%,极显著高于对照组的39.75%±4.283%(P<0.01);试验组小鼠溶血空斑形成数量为23.1±2.1318,极显著高于对照组的17.4±2.0111(P<0.01);试验组小鼠血清溶血素水平为262.2900±10.4826,显著高于对照组的231.2710±39.6115(P<0.05)。结果表明,“畜禽用多种微量元素注射液”能显著提高小鼠的免疫功能。
4.“畜禽用多种微量元素注射液”对羔羊贫血的治疗试验将临床诊断为贫血的40只羔羊,分为“畜禽用多种微量元素注射液”治疗组和药物对照治疗组,“畜禽用多种微量元素注射液”治疗组分为高、中(推荐剂量、)低三个试验组,每组试验动物10只,分别按每次1.0、0.75、0.5mL/kg进行治疗,一周两次,连续注射2次为一个疗程。以临床症状和血液学指标的改变为依据,判定“畜禽用多种微量元素注射液”对羔羊贫血的治疗效果。结果显示,经“畜禽用多种微量元素注射液”的治疗,贫血羔羊的临床症状消失或明显改善,血液学指标也有一定的改善。
“Injection of Trace Elements for Livestock and poultry”is the injection of a variety of trace elements which is made according to balancing supply principle. Pharmacodynamics and clinical trials of“Injection of Trace Elements for Livestock and poultry”in lambs were studied in thispaper to further provide the basis for clinical application and observe its effect on goat blood parameters and immune function.
1. Effects of Injection of Trace Elements for Livestock and poultry”on Physiological and biochemical indices of blood in lamb In the experiment, 20 goats(2-month-old) were randomly divided into the control group and the experimental group, 10 in each group, and then anticoagulant was taken from anterior vena cava before administration as a blank control. Injection of Trace Elements for Livestock and poultry”and 0.9% Natrii Chloridun Injection were respectively intramuscular injectioned to the experimental group and the control group 0.8mL every week. The experiment was lasted eight weeks, and every 15 days anticoagulant was taken from anterior vena cava. The result showed that the number of red blood cells, white blood cells and lymphocytes and hemoglobin content could be increased significantly(P <0.05), SOD activity was significantly higher(P <0.05), the content of MDA decreased significantly (P <0.05), and the activity of GSH-Px was significantly increased (P <0.05) with Injection of Trace Elements for Livestock and poultry”.
2. The Effect of“Injection of Trace Elements for Livestock and poultry”on the Immune Function to goat and the experiment of gaining weigt The experiment is to study the effect of“Injection of Trace Elements for Livestock and poultry”on the immune function of lambs through the E-roses Ring tests and lymphocyte proliferation tests. The animals of treatment were same as anterior experiment. The peripheral blood lymphocytes were separated from anticoagulation, then the E-roses Ring tests and lymphocyte proliferation tests were carried on. Lamb weight was measured before and after test, and the test was lasted three months.The result indicated that the E-roses ring forming rate and the lymphocyte transformation rate in the experiment group which infected“Trace Elements Injection”are significantly higher than those of control group(P <0.05). It has been demonstrated that“Injection of Trace Elements for Livestock and poultry”play a significant role in promoting growth of lamb(P <0.01).
3. The Effect of“Injection of Trace Elements for Livestock and poultry”on the Immune Function to mice 60 Mice were randomly divided into three groups, and there are 20 mice in every group, meanwhile 20 mice were averagely divided into control group and test group. Mice in test group were injected intraperitoneally with 0.04mL“Trace Elements Injection”, and mice in control group with the same volume saline water once a day, continuously seven days. The ANAE test, hemolytic plaque test and content of serum hemolysin HC50 were carried out. The percentage of positive ANAE cell(51.80%±3.645%) in test group is extremely obviously higher than that of control group(39.75%±4.283%)(P<0.01). Hemolytic plaque quantity(23.1±2.1318) in test group is extremely obviously higher than that of control group(17.4±2.0111)(P<0.01).Serum hemolysin(262.2900±10.4826) in test group is obviously higher than that of control group(231.2710±39.6115)(P<0.05). These results suggested that“Trace Elements Injection”can obviously elevate immunologic funtion of mice.
4. Influence of the“Injection of Trace Elements for Livestock and poultry”on anaemia The 40 lambs diagnosed as anemia were divided into experimental group(30 lambs) injected with“Injection of Trace Elements for Livestock and poultry”and control group(10 lambs). The experimental group was further divided into three groups (10 lambs in each group), of which each were treated according to 1.0, 0.75, 0.5 mL / kg (high, medium ( recommended dose), low) respectively twice a week, and a week as a treatment course. In the basis of clinical symptoms and changes in blood indices, the treatmental effection of the drug on lamb anaemia was evaluated. The results showed that through the treatment of“Injection of Trace Elements in Domestic Animal and Poultry”, the clinical symptoms of anemia lambs disappeared or markedly improved, blood parameters also had improved, which suggested the drug play a significant role in treateent anemia of lambs.
1.“畜禽用多种微量元素注射液”对羔羊血液学及生理生化指标影响20只2月龄羔羊,随机分为对照组和试验组,每组10只,给药前静脉采抗凝血作为空白对照。试验组与对照组每周肌肉注射畜禽用多种微量元素注射液与生理盐水0.8 mL,连续8周。前腔静脉采血,测定血液中的红细胞、白细胞、淋巴细胞的数量和血红蛋白的含量以及血清中过氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)含量。结果表明,山羊注射多种微量元素注射液后,可显著使其红细胞、白细胞、淋巴细胞的数量及血红蛋白的含量升高(P<0.05);可使山羊血清中SOD的活性显著升高(P<0.05),使MDA的含量显著降低(P<0.05),使GSH-Px的活性显著升高(P<0.05)。
2.“畜禽用多种微量元素注射液”对羔羊免疫功能及增重的影响通过E玫瑰花环试验和淋巴细胞转化增殖试验研究了“畜禽用多种微量元素注射液”对羔羊免疫功能的影响。动物分组与处理同前。给药后每隔15 d静脉采血一次,抗凝处理,分离外周血淋巴细胞,进行E-玫瑰花环试验和淋巴细胞转化增殖试验。试验前和试验后1个月、2个月分别称量体重一次,进行增重试验。试验结果表明,使用多种微量元素注射液的实验组的E-玫瑰花环形成率和淋巴细胞的转化率显著高于对照组(P<0.05);同时对羔羊有显著的促生长作用(P<0.01)。
3.“畜禽用多种微量元素注射液”对小鼠免疫功能的影响将60只昆明小鼠(体重为20±2 g,雌雄各半)随机分为3组,每组20只。再将每组的20只小鼠分成试验组和对照组,每组10只,试验组每天腹腔注射0.04 mL“多种微量元素注射液”,对照组注射0.04 mL生理盐水,连续注射7 d。三组分别进行酸性α-醋酸萘脂酶法试验,溶血空斑试验和血清溶血素抗体(HC50)测定。结果显示,试验组小鼠ANAE阳性细胞百分比为51.80%±3.645%,极显著高于对照组的39.75%±4.283%(P<0.01);试验组小鼠溶血空斑形成数量为23.1±2.1318,极显著高于对照组的17.4±2.0111(P<0.01);试验组小鼠血清溶血素水平为262.2900±10.4826,显著高于对照组的231.2710±39.6115(P<0.05)。结果表明,“畜禽用多种微量元素注射液”能显著提高小鼠的免疫功能。
4.“畜禽用多种微量元素注射液”对羔羊贫血的治疗试验将临床诊断为贫血的40只羔羊,分为“畜禽用多种微量元素注射液”治疗组和药物对照治疗组,“畜禽用多种微量元素注射液”治疗组分为高、中(推荐剂量、)低三个试验组,每组试验动物10只,分别按每次1.0、0.75、0.5mL/kg进行治疗,一周两次,连续注射2次为一个疗程。以临床症状和血液学指标的改变为依据,判定“畜禽用多种微量元素注射液”对羔羊贫血的治疗效果。结果显示,经“畜禽用多种微量元素注射液”的治疗,贫血羔羊的临床症状消失或明显改善,血液学指标也有一定的改善。
“Injection of Trace Elements for Livestock and poultry”is the injection of a variety of trace elements which is made according to balancing supply principle. Pharmacodynamics and clinical trials of“Injection of Trace Elements for Livestock and poultry”in lambs were studied in thispaper to further provide the basis for clinical application and observe its effect on goat blood parameters and immune function.
1. Effects of Injection of Trace Elements for Livestock and poultry”on Physiological and biochemical indices of blood in lamb In the experiment, 20 goats(2-month-old) were randomly divided into the control group and the experimental group, 10 in each group, and then anticoagulant was taken from anterior vena cava before administration as a blank control. Injection of Trace Elements for Livestock and poultry”and 0.9% Natrii Chloridun Injection were respectively intramuscular injectioned to the experimental group and the control group 0.8mL every week. The experiment was lasted eight weeks, and every 15 days anticoagulant was taken from anterior vena cava. The result showed that the number of red blood cells, white blood cells and lymphocytes and hemoglobin content could be increased significantly(P <0.05), SOD activity was significantly higher(P <0.05), the content of MDA decreased significantly (P <0.05), and the activity of GSH-Px was significantly increased (P <0.05) with Injection of Trace Elements for Livestock and poultry”.
2. The Effect of“Injection of Trace Elements for Livestock and poultry”on the Immune Function to goat and the experiment of gaining weigt The experiment is to study the effect of“Injection of Trace Elements for Livestock and poultry”on the immune function of lambs through the E-roses Ring tests and lymphocyte proliferation tests. The animals of treatment were same as anterior experiment. The peripheral blood lymphocytes were separated from anticoagulation, then the E-roses Ring tests and lymphocyte proliferation tests were carried on. Lamb weight was measured before and after test, and the test was lasted three months.The result indicated that the E-roses ring forming rate and the lymphocyte transformation rate in the experiment group which infected“Trace Elements Injection”are significantly higher than those of control group(P <0.05). It has been demonstrated that“Injection of Trace Elements for Livestock and poultry”play a significant role in promoting growth of lamb(P <0.01).
3. The Effect of“Injection of Trace Elements for Livestock and poultry”on the Immune Function to mice 60 Mice were randomly divided into three groups, and there are 20 mice in every group, meanwhile 20 mice were averagely divided into control group and test group. Mice in test group were injected intraperitoneally with 0.04mL“Trace Elements Injection”, and mice in control group with the same volume saline water once a day, continuously seven days. The ANAE test, hemolytic plaque test and content of serum hemolysin HC50 were carried out. The percentage of positive ANAE cell(51.80%±3.645%) in test group is extremely obviously higher than that of control group(39.75%±4.283%)(P<0.01). Hemolytic plaque quantity(23.1±2.1318) in test group is extremely obviously higher than that of control group(17.4±2.0111)(P<0.01).Serum hemolysin(262.2900±10.4826) in test group is obviously higher than that of control group(231.2710±39.6115)(P<0.05). These results suggested that“Trace Elements Injection”can obviously elevate immunologic funtion of mice.
4. Influence of the“Injection of Trace Elements for Livestock and poultry”on anaemia The 40 lambs diagnosed as anemia were divided into experimental group(30 lambs) injected with“Injection of Trace Elements for Livestock and poultry”and control group(10 lambs). The experimental group was further divided into three groups (10 lambs in each group), of which each were treated according to 1.0, 0.75, 0.5 mL / kg (high, medium ( recommended dose), low) respectively twice a week, and a week as a treatment course. In the basis of clinical symptoms and changes in blood indices, the treatmental effection of the drug on lamb anaemia was evaluated. The results showed that through the treatment of“Injection of Trace Elements in Domestic Animal and Poultry”, the clinical symptoms of anemia lambs disappeared or markedly improved, blood parameters also had improved, which suggested the drug play a significant role in treateent anemia of lambs.
引文
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